ES2094972T3 - Procedimiento para obtencion de fosfatasa alcalina eucariotica recombinante, biologicamente activa. - Google Patents

Procedimiento para obtencion de fosfatasa alcalina eucariotica recombinante, biologicamente activa.

Info

Publication number
ES2094972T3
ES2094972T3 ES93112289T ES93112289T ES2094972T3 ES 2094972 T3 ES2094972 T3 ES 2094972T3 ES 93112289 T ES93112289 T ES 93112289T ES 93112289 T ES93112289 T ES 93112289T ES 2094972 T3 ES2094972 T3 ES 2094972T3
Authority
ES
Spain
Prior art keywords
alkaline phosphatase
biologically active
procedure
obtaining recombinant
recombinant alkaline
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES93112289T
Other languages
English (en)
Inventor
Uwe Dr Michaelis
Rainer Prof Rudolph
Michael Dr Jarsch
Erhard Dr Kopetzki
Helmut Dr Burtscher
Gunther Dr Schumacher
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Application granted granted Critical
Publication of ES2094972T3 publication Critical patent/ES2094972T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)

Abstract

EN UN PROCEDIMIENTO PARA OBTENER FOSFATASA ALCALINA EUROCAROTICA, ACTIVA BIOLOGICAMENTE Y RECOMBINANTE, SE EXPRESA UNA SECUENCIA DNA CODIFICANDO UNA FOSFATASA ALCALINA EUROCARIOTICA EN UNA CELULA ACTIVA PROCARIOTICA Y EL PRODUCTO DE EXPRESION SE OBTIENE EN FORMA ACTIVA A TRAVES DE DISGREGACION DE CELULAS, SOLUBILIZACION EN BAJAS CONDICIONES DE DESNATURALIZACION Y A CONTINUACION RENATURALIZACION. LA ETAPA DE RENATURALIZACION SE REALIZA EN PRESENCIA DE UNOS O VARIOS MEDIOS DE ESTABILIZACION.
ES93112289T 1992-07-31 1993-07-30 Procedimiento para obtencion de fosfatasa alcalina eucariotica recombinante, biologicamente activa. Expired - Lifetime ES2094972T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE4225427A DE4225427A1 (de) 1992-07-31 1992-07-31 Verfahren zur Gewinnung rekombinanter, biologisch aktiver, eukaryontischer alkalischer Phosphatase

Publications (1)

Publication Number Publication Date
ES2094972T3 true ES2094972T3 (es) 1997-02-01

Family

ID=6464618

Family Applications (1)

Application Number Title Priority Date Filing Date
ES93112289T Expired - Lifetime ES2094972T3 (es) 1992-07-31 1993-07-30 Procedimiento para obtencion de fosfatasa alcalina eucariotica recombinante, biologicamente activa.

Country Status (9)

Country Link
US (1) US5434067A (es)
EP (1) EP0586872B1 (es)
JP (1) JPH0824572B2 (es)
AT (1) ATE143668T1 (es)
DE (2) DE4225427A1 (es)
DK (1) DK0586872T3 (es)
ES (1) ES2094972T3 (es)
FI (1) FI933413A (es)
GR (1) GR3021233T3 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10511840A (ja) 1994-04-29 1998-11-17 クリエイティブ バイオモレキュールズ，インコーポレイテッド 形態形成タンパク質特異細胞表面レセプターおよびその使用
US5872141A (en) * 1997-02-18 1999-02-16 Umbreit; Jay N. Method of inhibiting cholesterol transport
WO2000040610A1 (fr) * 1999-01-07 2000-07-13 Takeda Chemical Industries, Ltd. Procede de production de proteines activees
DE10105912A1 (de) * 2001-02-09 2002-08-14 Roche Diagnostics Gmbh Rekombinante Proteinase K
CA2433479A1 (en) * 2002-07-22 2004-01-22 F. Hoffmann-La Roche Ag Conjugate of a tissue non-specific alkaline phosphatase and dextran, process for its production and use thereof
EP1405907A3 (en) * 2002-07-22 2004-10-13 Roche Diagnostics GmbH Conjugate of a tissue non-specific alkaline phosphatase and dextran, process for its production and use thereof
EP1745068A4 (en) * 2004-03-30 2009-11-04 Sudershan Biotech Ltd RECOMBINANT CALM CHYMOSIN AND PROCESS FOR PRODUCING THE SAME

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU1082811A1 (ru) * 1982-03-04 1984-03-30 Астраханский государственный медицинский институт им.А.В.Луначарского Способ выделени термостабильной плацентарной щелочной фосфатазы
GB8328918D0 (en) * 1983-10-28 1983-11-30 Unilever Plc Alkaline phosphatase
DD298424A5 (de) * 1989-11-22 1992-02-20 Universitaet Leipzig,De Verfahren zur gewinnung von alkalischer phosphatase aus kaelberdarm

Also Published As

Publication number Publication date
FI933413A0 (fi) 1993-07-30
EP0586872A1 (de) 1994-03-16
ATE143668T1 (de) 1996-10-15
DE4225427A1 (de) 1994-02-03
JPH06153938A (ja) 1994-06-03
US5434067A (en) 1995-07-18
JPH0824572B2 (ja) 1996-03-13
DE59304038D1 (de) 1996-11-07
EP0586872B1 (de) 1996-10-02
DK0586872T3 (es) 1997-03-17
FI933413A (fi) 1994-02-01
GR3021233T3 (en) 1997-01-31

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