DE69133012T2 - Verfahren und Zusammensetzung zur Steigerung der Akkumulation von Squalen und spezifischen Sterolen in Hefe - Google Patents

Verfahren und Zusammensetzung zur Steigerung der Akkumulation von Squalen und spezifischen Sterolen in Hefe

Info

Publication number
DE69133012T2
DE69133012T2 DE69133012T DE69133012T DE69133012T2 DE 69133012 T2 DE69133012 T2 DE 69133012T2 DE 69133012 T DE69133012 T DE 69133012T DE 69133012 T DE69133012 T DE 69133012T DE 69133012 T2 DE69133012 T2 DE 69133012T2
Authority
DE
Germany
Prior art keywords
squalene
yeast
accumulation
increasing
composition
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69133012T
Other languages
English (en)
Other versions
DE69133012D1 (de
Inventor
Court A Saunders
Fred Richard Wolf
Indrani Mukharji
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BP Corp North America Inc
Original Assignee
BP Corp North America Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BP Corp North America Inc filed Critical BP Corp North America Inc
Application granted granted Critical
Publication of DE69133012D1 publication Critical patent/DE69133012D1/de
Publication of DE69133012T2 publication Critical patent/DE69133012T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C12N1/185—Saccharomyces isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/85—Saccharomyces
    • C12R2001/865—Saccharomyces cerevisiae

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
DE69133012T 1990-11-15 1991-11-14 Verfahren und Zusammensetzung zur Steigerung der Akkumulation von Squalen und spezifischen Sterolen in Hefe Expired - Lifetime DE69133012T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US61338090A 1990-11-15 1990-11-15
US07/783,861 US5460949A (en) 1990-11-15 1991-10-28 Method and composition for increasing the accumulation of squalene and specific sterols in yeast

Publications (2)

Publication Number Publication Date
DE69133012D1 DE69133012D1 (de) 2002-06-27
DE69133012T2 true DE69133012T2 (de) 2003-01-02

Family

ID=27087007

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69133012T Expired - Lifetime DE69133012T2 (de) 1990-11-15 1991-11-14 Verfahren und Zusammensetzung zur Steigerung der Akkumulation von Squalen und spezifischen Sterolen in Hefe

Country Status (6)

Country Link
US (1) US5460949A (de)
EP (1) EP0486290B1 (de)
JP (1) JP3283551B2 (de)
DE (1) DE69133012T2 (de)
DK (1) DK0486290T3 (de)
ES (1) ES2176177T3 (de)

Families Citing this family (55)

* Cited by examiner, † Cited by third party
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US5741898A (en) * 1994-09-22 1998-04-21 Hanley; Kathleen Marie DNA sequence encoding nicotiana squalene synthetase
US20020086380A1 (en) * 1996-03-29 2002-07-04 Francis X. Cunningham Jr Genes encoding epsilon lycopene cyclase and method for producing bicyclic carotene
DE19744212B4 (de) * 1997-09-30 2006-01-19 Schering Ag Verfahren zur Herstellung von Ergosterol und dessen Zwischenprodukten mittels rekombinanter Hefen
EP0955363A3 (de) * 1998-05-06 2004-01-28 F. Hoffmann-La Roche Ag DNS Sequenzen kodierend für Enzyme für die Herstellung von Isoprenoiden
TWI250210B (en) 1998-05-06 2006-03-01 Dsm Ip Assets Bv An isolated DNA sequence coding for an enzyme involved in the mevalonate pathway or the pathway from isopentenyl pyrophosphate to farnesyl pyrophosphate
US6410755B1 (en) 1998-07-06 2002-06-25 Dcv, Inc. Method of vitamin production
EP2305825B1 (de) * 1998-07-06 2015-01-14 DCV Inc. doing business as Bio-Technical Resourses Verfahren zur Vitaminherstellung
WO2000001650A1 (en) * 1998-07-06 2000-01-13 Dcv, Inc. Doing Business As Bio-Technical Resources Method of vitamin production
JP2002519417A (ja) 1998-07-06 2002-07-02 イーストマン ケミカル カンパニー ビタミンｅの製造方法
US6399796B2 (en) 2000-03-17 2002-06-04 Roche Vitamins Inc. Activation of a Diels-Alder reaction of a sterol 5,7-diene
US7009045B2 (en) * 2000-07-14 2006-03-07 Archer-Daniels-Midland Company Transformation systems for flavinogenic yeast
JP3838033B2 (ja) * 2000-12-28 2006-10-25 トヨタ自動車株式会社 プレニルアルコールの製造方法
WO2002053747A1 (en) * 2000-12-28 2002-07-11 Toyota Jidosha Kabushiki Kaisha Process for producing prenyl alcohol
JP3894119B2 (ja) * 2000-12-28 2007-03-14 トヨタ自動車株式会社 プレニルアルコールの製造方法
US6822142B2 (en) * 2001-01-05 2004-11-23 Monsanto Company Transgenic plants containing altered levels of steroid compounds
DE10203352A1 (de) 2002-01-29 2003-07-31 Basf Ag Verfahren zur Herstellung von 7-Dehydrocholesterol und/oder dessen biosynthetischen Zwischen- und/oder Folgeprodukten in transgenen Organismen
DE10312314A1 (de) * 2003-03-19 2004-09-30 Basf Ag Verfahren zur Herstellung von Ergosta-5,7-dienol und/oder dessen biosynthetischen Zwischen- und/oder Folgeprodukten in transgenen Organismen
FR2869914B1 (fr) * 2004-05-06 2012-11-09 Aventis Pharma Sa Souches de levure produisant du cholesterol et leurs applications
MXPA06013502A (es) 2004-05-21 2007-03-01 Univ California Metodo para mejorar la produccion de compuestos isoprenoides.
EP1775304A4 (de) * 2004-07-13 2010-04-28 Mitsubishi Chem Corp Verfahren zur herstellung von steroiden
CA2574593C (en) 2004-07-27 2016-07-05 The Regents Of The University Of California Genetically modified host cells and use of same for producing isoprenoid compounds
EP2371967B1 (de) 2005-03-18 2015-06-03 DSM IP Assets B.V. Produktion von Carotenoiden in Ölhaltigen Hefen und Pilzen
BRPI0614990A2 (pt) * 2005-08-19 2011-04-26 Univ California células hospedeiras geneticamente modificadas e uso das mesmas para produção de compostos isoprenóides
JP2009511020A (ja) * 2005-10-07 2009-03-19 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 改変チトクロームｐ４５０酵素をコードする核酸およびその使用法
JP2007210888A (ja) 2006-01-12 2007-08-23 Mitsubishi Chemicals Corp ステロイド化合物の製造方法
US8114645B2 (en) * 2006-05-19 2012-02-14 The Regents Of The University Of California Methods for increasing isoprenoid and isoprenoid precursor production by modulating fatty acid levels
DK2024504T4 (da) 2006-05-26 2023-02-27 Amyris Inc Fremstilling af isoprenoider
WO2008130372A2 (en) 2006-09-28 2008-10-30 Microbia, Inc. Production of sterols in oleaginous yeast and fungi
US8691555B2 (en) 2006-09-28 2014-04-08 Dsm Ip Assests B.V. Production of carotenoids in oleaginous yeast and fungi
WO2009006429A1 (en) * 2007-06-29 2009-01-08 Regents Of The University Of California Host cells and methods for producing 3-methyl-2-buten-1-ol, 3-methyl-3-buten-1-ol, and 3-methyl-butan-1-ol
WO2009006386A2 (en) 2007-06-29 2009-01-08 The Regents Of The University Of California Host cells and methods for producing isoprenyl alkanoates
EP3260549A1 (de) * 2008-05-23 2017-12-27 Nucelis LLC Herstellung von squalen mittels hefe
AU2009286426B2 (en) 2008-08-28 2013-08-22 Novartis Ag Production of squalene from hyper-producing yeasts
DE102009022772A1 (de) 2009-05-21 2010-11-25 Organobalance Gmbh Mikroorganismus zur Expression eines humanen Membranproteins
JP4821886B2 (ja) * 2009-06-04 2011-11-24 トヨタ自動車株式会社 組換え酵母、当該組換え酵母を用いた分岐アルコールの製造方法
EP2449116A4 (de) * 2009-07-01 2013-12-11 Univ California Auszug aus extrazellulären terpenoiden aus mikroalgenkolonien
EP2292741A1 (de) 2009-08-26 2011-03-09 OrganoBalance GmbH Genetisch modifizierte Organismen zur Herstellung von Lipiden
JP5978132B2 (ja) 2009-11-23 2016-08-24 ニューセリス インコーポレイテッド 酵母を用いるスクアレン生成のための方法および組成物
WO2011067144A1 (en) 2009-12-03 2011-06-09 Dsm Ip Assets B.V. Production of non-yeast sterols by yeast
BR112012030836B8 (pt) 2010-06-02 2024-02-06 Evolva Nutrition Inc Hospedeiro recombinante que compreende genes recombinantes para produção de esteviol ou glicosídeo de esteviol, método para produzir esteviol, glicosídeo de esteviol ou composição de glicosídeo de esteviol e método para sintetizar esteviol ou glicosídeo de esteviol
PL2681313T3 (pl) 2011-02-28 2016-11-30 Komórka drożdżowa do wytwarzania terpenów i jej zastosowania
IN2014CN01129A (de) 2011-08-08 2015-04-10 Evolva Sa
WO2013022989A2 (en) 2011-08-08 2013-02-14 Evolva Sa Recombinant production of steviol glycosides
MY190346A (en) 2013-02-06 2022-04-15 Evolva Sa Methods for improved production of rebaudioside d and rebaudioside m
BR112015019160A2 (pt) 2013-02-11 2017-08-22 Dalgaard Mikkelsen Michael Produção de glicosídeos de esteviol em hospedeiros recombinantes
EP2971049B1 (de) * 2013-03-13 2017-03-01 Sanofi-Aventis Deutschland GmbH Herstellung von squalen und/oder sterol aus zellsuspensionen von fermentierter hefe
WO2015156369A1 (ja) * 2014-04-09 2015-10-15 株式会社Adeka 変異酵素及び前記変異酵素を用いたテルペノイドの製造方法
SG10201901007PA (en) 2014-08-11 2019-03-28 Evolva Sa Production of steviol glycosides in recombinant hosts
WO2016038095A2 (en) 2014-09-09 2016-03-17 Evolva Sa Production of steviol glycosides in recombinant hosts
CN108337892B (zh) 2015-01-30 2022-06-24 埃沃尔瓦公司 在重组宿主中生产甜菊醇糖苷
EP3862426A3 (de) 2015-03-16 2021-11-17 DSM IP Assets B.V. Udp-glycosyltransferasen
US10837041B2 (en) 2015-08-07 2020-11-17 Evolva Sa Production of steviol glycosides in recombinant hosts
CN109195457A (zh) 2016-04-13 2019-01-11 埃沃尔瓦公司 在重组宿主中产生甜菊醇糖苷
US10815514B2 (en) 2016-05-16 2020-10-27 Evolva Sa Production of steviol glycosides in recombinant hosts
CN110100006A (zh) 2016-11-07 2019-08-06 埃沃尔瓦公司 重组宿主中甜菊糖苷的生产

Also Published As

Publication number Publication date
US5460949A (en) 1995-10-24
JP3283551B2 (ja) 2002-05-20
EP0486290A2 (de) 1992-05-20
JPH05192184A (ja) 1993-08-03
EP0486290A3 (en) 1993-03-17
EP0486290B1 (de) 2002-05-22
DE69133012D1 (de) 2002-06-27
ES2176177T3 (es) 2002-12-01
DK0486290T3 (da) 2002-09-16

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