KR920703814A - 공유결합 안지오제닌/리보핵산가수분해효소 하이브라이드 - Google Patents
공유결합 안지오제닌/리보핵산가수분해효소 하이브라이드Info
- Publication number
- KR920703814A KR920703814A KR1019920701960A KR920701960A KR920703814A KR 920703814 A KR920703814 A KR 920703814A KR 1019920701960 A KR1019920701960 A KR 1019920701960A KR 920701960 A KR920701960 A KR 920701960A KR 920703814 A KR920703814 A KR 920703814A
- Authority
- KR
- South Korea
- Prior art keywords
- protein
- angiogenin
- amino acids
- host cell
- ribonuclease
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/515—Angiogenesic factors; Angiogenin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Public Health (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Vascular Medicine (AREA)
- Veterinary Medicine (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Cardiology (AREA)
- Heart & Thoracic Surgery (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Elevator Control (AREA)
Abstract
내용 없음
Description
공유결합 안지오제닌/리보핵산가수분해효소 하이브라이드
[도면의 간단한 설명]
제 1a 및 1b도(실시예 1에서 설명된)는 안지오제닌 고유분절을 결정할 목적으로 인간 안지오제닌의 잔기수를 소의 안지오제닌(제 1a도) 및 리보핵산가수분해효소 A(제 1b 도)의 잔기수에 비교함으로서 창출된 아미노산 배열의 % 동일성을 보여주는 그래프이다.
제 1c도는 제 1a 및 1b 도사이의 % 동일성의 상위성을 보여주는 그래프이다.
제 2a도는 리보핵산가수분해효소 A, 인간 안지오제닌, 및 ARH-Ⅲ의 N-말단 아미노산배열들을 보여준다. 39가지 포유류종들로부터 얻어진 췌장 리보핵산가수분해효소내에 보전되고 있는 리보핵산가수분해 효소 A내 잔기들은 별표로 나타내었다. 안지오제닌내의 사각형으로 둘러쳐진 잔기들은 인간, 소, 돼지, 및 토끼 단백질들에 보전되는 것이다.
제 2b도는 실시예 2에 선명된 바와같은 ARH-Ⅲ 코딩 배열의 축조시에 단편 B로서 사용되는 ARH-Ⅲ의 4-23 잔기들을 암호화하는 합성적 이본쇄올리고 뉴클레오타이드의 DNA 배열을 보여준다.
제 3도는 실시예 2에 설명된 바와같은 ARH-Ⅲ 코딩 배열의 축조에 관한 선도(線圖)이다.
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
Claims (15)
- 안지오제닌 잔기들 8-22번 지역(또는 8-22번에 해당하는 지역)내의 아미노산들을 다른 아미노산들로 대체시켜 만든 증대된 맥관형성촉진 활성을 지니는 돌연변이 안지오제닌 단백질.
- 제1항에 있어서, 안지오제닌 잔기를 8-22번 지역(또는 8-22번에 해당하는 지역)을 리보핵산가수분해효소 내 아미노산들로 이루어진 지역으로 대체시켜 만든 돌연변이 안지오제닌 단백질.
- 제2항에 있어서, 리보핵산가수분해효소내 아미노산들이 해당지역이 리보핵산가수분해효소 잔기들 7-21번 아미노산을(또는 7-21번에 해당하는 아미노산들)을 포함하는 돌연변이 안지오제닌 단백질.
- 제1항의 단백질을 암호화하고 있는 DNA 배열을 포함하는 정제분리된 DNA 배열.
- 제2항의 단백질을 암호화하고 있는 DNA 배열을 포함하는 정제분리된 DNA 배열.
- 제3항의 단백질을 암호화하고 있는 DNA 배열을 포함하는 정제분리된 DNA 배열.
- 형질 전환된 숙주세포내에서 제1항의 단백질을 발현시킬 목적으로 분리한 벡터.
- 형질 전환된 숙주 세포내에서 제2항의 단백질을 발현시킬 목적으로 분리한 벡터.
- 형질 전환된 숙주 세포내에서 제3항의 단백질을 발현시킬 목적으로 분리한 벡터.
- 제7항의 벡터로 형질 전환된 숙주세포.
- 제8항의 벡터로 형질 전환된 숙주세포.
- 제9항의 벡터로 형질 전환된 숙주세포.
- 약학적으로 용인되는 담체내에 제1항 단백질의 유효 맥관형성촉진량을 포함하는 제약조성물.
- 약학적으로 용인되는 담체내에 제2항 단백질의 유효 맥관형성촉진량을 포함하는 제약조성물.
- 약학적으로 용인되는 담체내에 제3항 단백질의 유효 맥관형성촉진량을 포함하는 제약조성물.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US48134290A | 1990-02-15 | 1990-02-15 | |
US07/481,342 | 1990-02-15 | ||
PCT/US1991/000113 WO1991012322A1 (en) | 1990-02-15 | 1991-01-07 | COVALENT ANGIOGENIN/RNase HYBRIDS |
Publications (1)
Publication Number | Publication Date |
---|---|
KR920703814A true KR920703814A (ko) | 1992-12-18 |
Family
ID=23911589
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019920701960A KR920703814A (ko) | 1990-02-15 | 1991-01-07 | 공유결합 안지오제닌/리보핵산가수분해효소 하이브라이드 |
Country Status (13)
Country | Link |
---|---|
US (1) | US5286487A (ko) |
EP (1) | EP0515394A1 (ko) |
JP (1) | JPH05504060A (ko) |
KR (1) | KR920703814A (ko) |
AU (1) | AU645055B2 (ko) |
CA (1) | CA2073434A1 (ko) |
CS (1) | CS29191A3 (ko) |
IE (1) | IE910465A1 (ko) |
IL (1) | IL96519A0 (ko) |
NZ (1) | NZ236375A (ko) |
PT (1) | PT96574A (ko) |
WO (1) | WO1991012322A1 (ko) |
ZA (1) | ZA909633B (ko) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5910481A (en) * | 1995-11-13 | 1999-06-08 | Immuno Ag | Hybrid proteins with modified activity |
US5852001A (en) * | 1996-12-23 | 1998-12-22 | The Endowment For Research In Human Biology | Method and compounds for inhibition of ribonucleases |
WO1999006569A1 (en) * | 1997-07-30 | 1999-02-11 | Stichting Instituut Voor Dierhouderij En Diergezondheid | Cytotoxic polypeptide (fragment) derived from ribonuclease |
US8470315B2 (en) | 2004-04-13 | 2013-06-25 | Quintessence Biosciences, Inc. | Non-natural ribonuclease conjugates as cytotoxic agents |
JP2009541333A (ja) * | 2006-06-23 | 2009-11-26 | クインテセンス バイオサイエンシーズ インコーポレーティッド | 修飾リボヌクレアーゼ |
EP2049151A4 (en) | 2006-07-17 | 2010-03-24 | Quintessence Biosciences Inc | METHOD AND COMPOSITIONS FOR THE TREATMENT OF CANCER |
US7601689B2 (en) | 2007-04-12 | 2009-10-13 | Naidu Lp | Angiogenin complexes (ANGex) and uses thereof |
US8828698B2 (en) * | 2007-04-12 | 2014-09-09 | Naidu Lp | Immobilized angiogenin mixtures and uses thereof |
WO2009048909A1 (en) * | 2007-10-08 | 2009-04-16 | Quintessence Biosciences, Inc. | Compositions and methods for ribonuclease-based therapies |
CN102227443B (zh) | 2008-10-01 | 2014-05-14 | 昆特森斯生物科学公司 | 治疗性核糖核酸酶 |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4229531A (en) * | 1978-12-29 | 1980-10-21 | Monsanto Company | Production of tumor angiogenesis factor by cell culture |
US4503038A (en) * | 1983-02-25 | 1985-03-05 | The Regents Of The University Of California | Extracellular nonmitogenic angiogenesis factor and method of isolation thereof from wound fluid |
US4727137A (en) * | 1985-04-17 | 1988-02-23 | President And Fellows Of Harvard College | Purified protein having angiogenic activity and methods of preparation |
US4721672A (en) * | 1985-08-28 | 1988-01-26 | President And Fellows Of Harvard College | CDNA and gene for human angiogenin (angiogenesis factor) and methods of expression |
DE3716722A1 (de) * | 1987-05-19 | 1988-12-01 | Hoechst Ag | Gentechnologisches verfahren zur herstellung von angiogeninen |
US4900673A (en) * | 1988-03-28 | 1990-02-13 | President And Fellows Of Harvard College | Mutant human angiogenin (angiogenesis factor with superior angiogenin activity) genes therefor and methods of expression |
-
1990
- 1990-11-30 ZA ZA909633A patent/ZA909633B/xx unknown
- 1990-11-30 IL IL96519A patent/IL96519A0/xx unknown
- 1990-12-06 NZ NZ236375A patent/NZ236375A/en unknown
-
1991
- 1991-01-07 WO PCT/US1991/000113 patent/WO1991012322A1/en not_active Application Discontinuation
- 1991-01-07 CA CA002073434A patent/CA2073434A1/en not_active Abandoned
- 1991-01-07 EP EP91902577A patent/EP0515394A1/en not_active Withdrawn
- 1991-01-07 JP JP3502993A patent/JPH05504060A/ja active Pending
- 1991-01-07 AU AU71595/91A patent/AU645055B2/en not_active Expired - Fee Related
- 1991-01-07 KR KR1019920701960A patent/KR920703814A/ko not_active Application Discontinuation
- 1991-01-25 PT PT96574A patent/PT96574A/pt not_active Application Discontinuation
- 1991-02-07 CS CS91291A patent/CS29191A3/cs unknown
- 1991-02-12 IE IE046591A patent/IE910465A1/en unknown
-
1992
- 1992-09-29 US US07/953,555 patent/US5286487A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
NZ236375A (en) | 1992-06-25 |
WO1991012322A1 (en) | 1991-08-22 |
IE910465A1 (en) | 1991-08-28 |
CA2073434A1 (en) | 1991-08-16 |
JPH05504060A (ja) | 1993-07-01 |
US5286487A (en) | 1994-02-15 |
PT96574A (pt) | 1991-10-15 |
AU645055B2 (en) | 1994-01-06 |
IL96519A0 (en) | 1991-08-16 |
AU7159591A (en) | 1991-09-03 |
CS29191A3 (en) | 1992-06-17 |
EP0515394A1 (en) | 1992-12-02 |
ZA909633B (en) | 1991-10-30 |
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