EP3638692A1 - Method of manufacturing bispecific antibodies, bispecific antibodies and therapeutic use of such antibodies - Google Patents

Method of manufacturing bispecific antibodies, bispecific antibodies and therapeutic use of such antibodies

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Publication number
EP3638692A1
EP3638692A1 EP18742595.4A EP18742595A EP3638692A1 EP 3638692 A1 EP3638692 A1 EP 3638692A1 EP 18742595 A EP18742595 A EP 18742595A EP 3638692 A1 EP3638692 A1 EP 3638692A1
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EP
European Patent Office
Prior art keywords
amino acid
seq
acid sequence
sequence seq
immunoglobulin
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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EP18742595.4A
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German (de)
English (en)
French (fr)
Inventor
Michael Otto BARDROFF
Tina BUCH
Christian Graf
Daniel Heitmann
Thomas Jostock
Hans-Peter Knopf
Rolf Koehler
Jiri Kovarik
Stephen John OLIVER
Dhavalkumar Patel
Maximilian Woisetschlaeger
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Novartis AG
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Novartis AG
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Publication of EP3638692A1 publication Critical patent/EP3638692A1/en
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C07K16/468—Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/14—Vasoprotectives; Antihaemorrhoidals; Drugs for varicose therapy; Capillary stabilisers
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/244—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/244—Interleukins [IL]
    • C07K16/245—IL-1
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2863—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for growth factors, growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2887—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against CD20
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/51—Complete heavy chain or Fd fragment, i.e. VH + CH1
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/515—Complete light chain, i.e. VL + CL
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C07K2317/526—CH3 domain
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C07K2317/53—Hinge
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Definitions

  • the invention relates to bivalent bispecific monoclonal antibodies (bbmAb) or variants thereof, and methods of manufacturing such antibodies by co-expressing so called knob-into-hole modified FC-mutated derivatives of two different monoclonal antibodies in mammalian cell lines.
  • Bispecific antibodies i.e. antibodies binding to two distinct epitopes
  • One approach for generating bispecific antibodies is the so called knobs-into-holes (KiH) approach described e.g. by Merchant et al., Nat. Biotechnol., 16:677-681 (1998), where a first heavy chain IgG is modified to display a hole like structure by introducing point mutations like Y349C, T366S, L368A, Y407V ; and where a second heavy chain IgG is modified to display a knob like structure, by introducing point mutations S354C, T366W ((Merchant et al., Nat.
  • bbmAb bivalent bispecific antibody
  • the desired bbmAb When expressing two KiH modified mAbs in the same host cell line, the desired bbmAb statistically makes up only 25% of the expressed protein, but 75% are so called product related impurities (Klein, Ch. et al., 2012).
  • bivalent bispecific antibodies there is a need to provide an improved method for manufacturing of bivalent bispecific antibodies. Particularly, there is a need for a method for manufacturing of bivalent bispecific monoclonal antibodies (bbmAb), ensuring a sufficient overall yield, purity and product quality to proceed with clinical development and commercial manufacturing, at a reasonable cost.
  • bbmAb bivalent bispecific monoclonal antibodies
  • the present invention provides inter alia a method for the generation of bbmAbs with one or more of the following advantages: it enables the use of a large antibody repertoire to identify binders as no shared light or heavy chains are required, it does not require any extensive protein engineering, beside the mutation driving the H-chain dimerization, and therefore limits the risk for anti-drug antibodies, it is cost effective as expression is done in a common cell line, therefore the bbmAb can be produced in one cell culture process without the need for a specific in vitro shuffling and it produces high quality material suitable for human use as product related impurities can be efficiently removed.
  • the present invention is useful for identifying antibodies of kappa and lambda type, where the light chains do not show a strong promiscuous binding towards the heavy chain of the counterpart. This makes the antibodies suitable for use in methods of the invention.
  • An advantage of the method may be that antibody combinations where both light chains exchange the original heavy chain binding partner, which results in a product related impurity of the H1 L2-H2L1 type, can be deselected. This is
  • embodiments of the invention enable manufacturing of bbmAb by the use of a CHO co-expression at a yield and quality suitable for clinical development and commercialization of biologies.
  • a bispecific antibody suitable for co-expression in a common host cell comprising a) a first part which is an immunoglobulin with a variable light chain of lambda wild type (VL1) and a variable heavy chain of wild type (VH1), that binds specifically to a first target, and a first constant heavy chain (CH1) with a hetero-dimerization modification, and b) a second part which is an immunoglobulin with a variable light chain of kappa wild type (L2) and a variable heavy chain of wild type (H2), that binds specifically to a second target, different from the first target, and a second constant heavy chain (CH2) with a hetero-dimerization modification which is complementary to the hetero-dimerization modification of the first constant heavy chain, wherein the first part and the second part, when co-expressed in a common host cell, form a bispecific antibody.
  • VL1 variable light chain of lambda wild type
  • VH1 variable heavy chain of wild type
  • CH1 first constant
  • the bispecific antibody suitable for co- expression in a common host cell results, after purifying the bispecific antibody by removing mismatched fragments from the correctly matched bispecific antibody, in a bispecific antibody which is at least 60% (mass), 70% (mass), 80% (mass), 85% (mass) pure, such as at least 90% (mass) pure, 95% (mass), 96% (mass), 97% (mass), 98% (mass), or 99% (mass) pure.
  • the first and second constant heavy chain of the bispecific antibody may be human IgA, IgD, IgE, IgG, or IgM, preferably IgD, IgE or IgG.
  • the first and second constant heavy chains are human lgG1 , lgG2, lgG3, or lgG4, most preferably IgGl
  • the first variable light chain is of lamba type
  • the second variable light chain is of kappa type.
  • the first variable light chain is of lambal type
  • the second variable light chain is of kappa 6 type.
  • the first and second constant heavy chain may be lgG1 , wherein the first constant heavy chain has point mutations generating a knob structure and the second constant heavy has point mutations generating a hole structure, or the first constant heavy chain has point mutations generating a hole structure and the second constant heavy has point mutations generating a knob structure.
  • the first and second constant heavy chains can additionally have mutations resulting in a disulfide bridge.
  • the bispecific antibody comprises a first immunoglobulin VH1 domain, a first immunoglobulin VL1 domain, a second immunoglobulin VH2 domain and a second immunoglobulin VL2 domain, wherein the first immunoglobulin VH1 domain comprises (e.g.
  • the second immunoglobulin VH2 domain comprises (e.g. in sequence):
  • the bispecific antibody comprises a first immunoglobulin VH1 domain, a first immunoglobulin VL1 domain, a second immunoglobulin VH2 domain and a second immunoglobulin VL2 domain, wherein: the first immunoglobulin VH1 domain comprises the amino acid sequence SEQ ID NO: 85, the first immunoglobulin VL1 domain comprises the amino acid sequence SEQ ID NO: 101 , the second immunoglobulin VH1 domain comprises the amino acid sequence SEQ ID NO: 85, the first immunoglobulin VL1 domain comprises the amino acid sequence SEQ ID NO: 101 , the second immunoglobulin VH1 domain comprises the amino acid sequence SEQ ID NO: 85, the first immunoglobulin VL1 domain comprises the amino acid sequence SEQ ID NO: 101 , the second immunoglobulin VH1 domain comprises the amino acid sequence SEQ ID NO: 85, the first immunoglobulin VL1 domain comprises the amino acid sequence SEQ ID NO: 101 , the second immunoglobulin VH1 domain comprises the amino acid sequence SEQ ID
  • the bispecific antibody comprises a first immunoglobulin heavy chain, a first immunoglobulin light chain, a second immunoglobulin heavy chain and a second immunoglobulin light chain, wherein: the first immunoglobulin heavy chain comprises the amino acid sequence SEQ ID NO: 87, the first immunoglobulin light chain comprises the amino acid sequence SEQ ID NO: 103, the second immunoglobulin heavy chain comprises the amino acid sequence SEQ ID NO: 55, the second immunoglobulin light chain comprises the amino acid sequence SEQ ID NO: 71.
  • a method for selecting a bispecific antibody according to the first aspect comprising; a first step of selecting the first part, and the second part; a second step of co-expressing the first part and the second part in a common host cell, resulting in a bispecific antibody comprising the first part and the second part; a third step of purifying the bispecific antibody by removing mismatched fragments from the correctly matched bispecific antibody.
  • the third step of purification results in a bispecific antibody which is at least 60% (mass), 70% (mass), 80% (mass), 85% (mass) pure, such as at least 90% (mass) pure, 95% (mass), 96% (mass), 97% (mass), 98% (mass), or 99% (mass) pure.
  • a method for manufacturing a bispecific antibody according to the first aspect by co-expression in a common host cell comprising; a first step of generating at least one vector encoding the first part and the second part; a second step of introducing the at least one vector into the common host cell; a third step of selecting cells specifically expressing the bispecific antibody; a forth step of culturing the selected cells under conditions wherein the cells express the bispecific antibody; and a fifth step of purifying the bispecific antibody which is at least 60% (mass), 70% (mass), 80% (mass), 85% (mass) pure, such as at least 90% (mass) pure, 95% (mass), 96% (mass), 97% (mass), 98% (mass), or 99% (mass) pure.
  • the first step comprises generating a first vector encoding the first part and a second vector encoding the second part.
  • an expression system comprising at least one vector comprising a polynucleotide encoding the first part or the second part of the bispecific antibody according to the first aspect, and a selectable marker.
  • the expression system comprises a polynucleotide encoding a first selectable marker (sm I); and a polynucleotide encoding a second selectable marker (sm II), which differs from the first selectable marker (sm I).
  • the first selectable marker (sm I) is a folate transporter or a polynucleotide encoding a mutated folate receptor, wherein the mutated folate receptor has a decreased folate binding affinity compared to the wildtype folate receptor and the second selectable marker (sm II) is DHFR.
  • the first selectable marker (sm I) is Hygromycine and the second selectable marker (sm II) is Neo/G418.
  • the expression system comprises two expression vectors wherein: a first vector comprising polynucleotide encoding at least a first selectable marker (sm I) and at least polynucleotides encoding the first part; and a second vector comprising polynucleotide encoding at least a second selectable marker (sm II) and at least polynucleotides encoding the second part.
  • a first vector comprising polynucleotide encoding at least a first selectable marker (sm I) and at least polynucleotides encoding the first part
  • a second vector comprising polynucleotide encoding at least a second selectable marker (sm II) and at least polynucleotides encoding the second part.
  • the expression system may comprise a stop codon downstream of the polynucleotides encoding the heavy chain and a polynucleotide encoding an
  • immunoglobulin membrane anchor located downstream of the stop codon.
  • a method for selecting a common host cell for use in a method according to previous aspects comprising a first step of providing a plurality of host cells, comprising the expression system according to previous aspects; and culturing said plurality of host cells under conditions selective for the selectable marker, thereby obtaining a host cell expressing the product of interest.
  • the selective culture medium is selected from the group comprising a medium comprising a limiting concentration of folate; and/or comprising a folic acid in a concentration of 500nM or less; and/or comprising a folic acid in a concentration selected from: 1000 nM - 100 pM; 100 nM - 1 nM; 15nM - 1 nM; 10nM - 1 nM; and 10 nM - 2.5 nM; and/or comprising a DHFR inhibitor; and/or comprising an antifolate; and/or comprising an antifolate in a concentration of 500 nM or less; and/or comprising MTX in a concentration selected from: 500 nM - 3 nM; 100 nM - 10 nM; 50nM - 10nM; and 50 nM; and/or comprising a concentration of antifolate up to 20-fold of the folate concentration; and/or comprising a concentration of antif
  • the host cell comprises the expression system wherein at least a portion of the first or second part is expressed as a fusion polypeptide comprising the immunoglobulin transmembrane anchor or fragment thereof, wherein said fusion polypeptide is being displayed on the surface of said host cell, further comprising a step of: contacting the plurality of host cells with a detection compound binding the fusion polypeptide; selecting at least one host cell based upon the presence or amount of the detection compound bound to the cell surface.
  • the detection compound comprises the first or second target, or derivatives thereof, and a detection label.
  • the fifth step of purifying the bispecific antibody comprises affinity chromatography and/or ion exchange chromatography.
  • the chromatography comprises a first step of capturing; a second step of polishing; and optionally a third step of polishing.
  • the first step of capturing is performed with a principle selected the group consisting of Fc-binding affinity chromatography, such as Protein A or Protein G, lambda light chain specific affinity chromatography, well known in the art, and readily available commercially, for example LambdaFabSelectTM, kappa light chain specific affinity chromatography, well known in the art, and readily available
  • Fc-binding affinity chromatography such as Protein A or Protein G
  • lambda light chain specific affinity chromatography well known in the art, and readily available commercially, for example LambdaFabSelectTM, kappa light chain specific affinity chromatography, well known in the art, and readily available
  • KappaSelectTM anti-idiotypic affinity chromatography, such as the first part or the second part
  • a target based affinity chromatography such as affinity chromatography using the first target or second target
  • ion exchange chromatography well known in the art, and readily available commercially, for example CaptoTM adhere, or FractogelTM EMD SO3, and hydrophobic interaction chromatography.
  • the second step of polishing is performed with a principle selected the group consisting of Fc-binding affinity chromatography, such as Protein A or Protein G, lambda light chain specific affinity chromatography, such as
  • LambdaFabSelectTM kappa light chain specific affinity chromatography, such as
  • KappaSelectTM anti-idiotypic affinity chromatography, such as the first part or the second part, a target based affinity chromatography, such as affinity chromatography using the first target or second target, ion exchange chromatography, such as CaptoTM adhere, or FractogelTM EMD SO 3 , hydrophobic interaction chromatography, and virus inactivation.
  • the third step of polishing is performed with a principle selected the group consisting of Fc-binding affinity chromatography, such as Protein A or Protein G, lambda light chain specific affinity chromatography, such as
  • LambdaFabSelectTM kappa light chain specific affinity chromatography, such as
  • KappaSelectTM anti-idiotypic affinity chromatography, such as the first part or the second part, a target based affinity chromatography, such as affinity chromatography using the first target or second target, ion exchange chromatography, such as CaptoTM adhere, or FractogelTM EMD SO3, hydrophobic interaction chromatography, and virus inactivation.
  • the method comprises a first step of Protein A capturing, such as MabSelectTM SuReTM, a second step of lambda light chain affinity chromatography, such as LambdaFabSelectTM, and a third step of kappa light chain affinity
  • MabSelectTM SuReTM a second step of kappa light chain affinity chromatography such as KappaSelectTM, and a third step of lambda light chain affinity chromatography, such as LambdaFabSelectTM; or a first step of kappa light chain affinity chromatography such as KappaSelectTMand a second step of lambda light chain affinity chromatography, such as LambdaFabSelectTM; or a first step of lambda light chain affinity chromatography, such as LambdaFabSelectTM and a second step of kappa light chain affinity
  • the cell line is selected from the group consisting of a CHO cell, a non-producing hybridoma, such as Sp 2/0 or NSO, a human derived cell line, such as HEK or PER.C6, a baby hamster kidney (BHK) derivative, a yeast or filamentous fungi, a prokaryotic bacteria, such as E. coli or Pseudomonas fluorescence, a plant derivative, an algae and a ciliate.
  • a CHO cell a non-producing hybridoma, such as Sp 2/0 or NSO
  • a human derived cell line such as HEK or PER.C6
  • BHK baby hamster kidney
  • yeast or filamentous fungi such as yeast or filamentous fungi
  • a prokaryotic bacteria such as E. coli or Pseudomonas fluorescence
  • a plant derivative such as E. coli or Pseudomonas fluorescence
  • an algae and a ciliate
  • a pharmaceutical composition comprising the antibody according to the first aspect and a pharmaceutically acceptable carrier.
  • an antibody according to a first aspect, or the pharmaceutical composition according to the sixth aspect, for use as a medicament is provided.
  • an antibody according to a first aspect, or the pharmaceutical composition according to the sixth aspect, for use as in the treatment of an inflammasome related disease is provided.
  • an antibody according to a first aspect, or the pharmaceutical composition according to the sixth aspect, for use as in the treatment of an inflammasome related disease is provided, wherein the inflammasome related disease is selected from the group consisting of sickle cell disease, vasculopathy, ischemia-reperfusion injury, cardiovascular disease, peripheral artery disease, atherosclerosis, vascular dysfunction, skeletal muscle ischemia, pulmonary sarcoidosis, fibrosis, malaria, hemodialysis-dependent, chronic kidney disease and Crohn's disease.
  • a method of treating an inflammasome related disorder comprising administering to a subject afflicted with a inflammasome related disorder an effective amount of an antibody according to the first aspect or a
  • the inflammasome related disorder may be sickle cell disease, vasculopathy, ischemia-reperfusion injury, cardiovascular disease, peripheral artery disease, atherosclerosis, vascular dysfunction, skeletal muscle ischemia, pulmonary sarcoidosis, fibrosis, malaria, hemodialysis-dependent, chronic kidney disease or Crohn's disease.
  • Figure 1 is a schematic overview of the vector setup according to an
  • Figure 2A - 2E shows chromatograms according to an embodiment.
  • Figure 2A is a RP-UV chromatogram of a de-glycosylated intact bbmAb according to an embodiment.
  • Figure 2B is a de-convoluted mass spectrum of the intact de-glycosylated bbmAbl according to an embodiment.
  • Figure 2C is a RP-UV chromatogram showing papain digested fragments of a bbmAb according to an embodiment.
  • Figure 2D is a RP-UV chromatogram showing IdeS digested fragments of a bbmAb according to an
  • Figure 2E is a RP-UV chromatogram showing de-glycosylated and DTT reduced fragments of a bbmAb according to an embodiment.
  • Figure 3A - 3D shows RP-UV chromatograms according to an embodiment.
  • Figure 3A is a chromatogram showing the expression purity profile of a bbmAb according to an embodiment after cultivation.
  • Figure 3B is a chromatogram of a bbmAb according to an embodiment after capturing by LambdaFabSelectTM.
  • Figure 3C is a chromatogram of a bbmAb according to an embodiment after capturing with MabSelectTM SuReTM.
  • Figure 3D is a chromatogram of a bbmAb according to an embodiment after capturing with LambdaFabSelectTM, polish by FractogelTM EMD S0 3 and ultrafiltration.
  • Figure 4A - 4M is a schematic overview of different options for bispecific mismatching.
  • Figure 4A is a schematic representation of a mAbl knob (lambda) monomer, where number 1 represents the variable heavy domain, number 2 represents the first constant heavy domain, number 3 represents the second constant heavy domain and number 4 represents the third constant heavy domain.
  • Number 5 represents the variable light domain and number 6 represents the variable heavy domain.
  • Figure 4B is a schematic representation of a mAbl knob (lambda) homodimer.
  • Figure 4C is a schematic representation of a mAb2 hole (kappa) monomer, where number 7 represents the variable heavy domain, number 8 represents the first constant heavy domain, number 9 represents the second constant heavy domain, and number 10 represents the third constant heavy domain.
  • Number 11 represents the variable light domain and number 12 represents the constant heavy domain.
  • Figure 4D is a schematic representation of a mAbl knob (lambda) monomer, where number 1 represents the variable heavy domain, number 2
  • Figure 4E is a schematic
  • Figure 4F is a schematic representation of a mAbl knob homodimer with two CH/LC mispairings.
  • Figure 4G is a schematic representation of a mAbl knob homodimer with one CH/LC mispairing.
  • Figure 4H is a schematic representation of a mAb2 hole homodimer with one CH/LC mispairing.
  • Figure 4I is a schematic representation of a mAb2 hole homodimer with two CH/LC mispairings.
  • Figure 4J is a schematic representation of a mAb2 hole homodimer with one CH/LC mispairing.
  • Figure 4K is a schematic representation of bbmAbl with one kappa (CH/LC) mispairing.
  • Figure 4L is a schematic representation of bbmAbl with one lambda (CH/LC) mispairing.
  • Figure 4M is a schematic representation of bbmAbl with two CH/LC mispairings.
  • Figure 5 shows titration curves of ECL-based affinity determination according to an Example.
  • Figure 6A - 6B shows two graphs according to an Example.
  • Figure 7A - 7B shows two graphs according to an Example.
  • Figure 8A - 8B shows mRNA expression levels according to an Example.
  • Figure 9A -9B shows mRNA expression levels according to an Example.
  • Figure 10A - 10B shows two graphs according to an Example.
  • Figure 1 1 is a graph showing statistical correlations according to an Example.
  • the present disclosure is inter alia based on the unexpected finding that certain antibodies with a light chain of lambda ( ⁇ ) type are possible to co-express with certain antibodies with a light chain of kappa ( ⁇ ) type, to form the desired bbmAb.
  • the CDRs of each light chain and/or heavy chain could significantly influence which light chain of lambda ( ⁇ ) type are possible to co-express with certain antibodies with a light chain of kappa ( ⁇ ) type to successfully obtain the bbmab formed.
  • the antibodies with a light chain of lambda ( ⁇ ) type that are possible to co- express with certain antibodies with a light chain of kappa ( ⁇ ) type, to form the desired bbmAb, in the following also called monospecific binders, can be generated either by use of technologies offering the opportunity to obtain both types of antibodies of either kappa or lambda type antibodies, such as Phage display libraries, e.g. HuUCAL GOLD® or HuCAL PLATINUM® (MorphoSys), or by use of transgenic mice, where the relevant human immunoglobulin sequences have been introduced into the genome of an animal by genetic engineering, e.g.
  • OmniAb antibodies (OMT), KymouseTM (Kymab), Trianni MouseTM (Trianni) or AlivaMab Mouse (Ablexis) (reference)can generate kappa or lambda type antibodies.
  • the methods to generate such monospecific binders are well known in the expert field, and are widely applied to generate diverse sets of either kappa or lambda type monospecific binders towards the relevant target of interest.
  • the individual monospecific binders are characterized with respect to relevant biological parameters such as affinity or potency, and also screened for e.g. physicochemical characteristics relevant to judge the so called developability characteristics which are also very well known in the field (e.g. Lorenz et al., American Pharmaceutical Review, August 2014).
  • Monospecific binders showing the best characteristics are finally co- expressed in e.g. CHO cells as described in more detail below. Only combinations are tested by co-expression where a kappa type antibody binding to the first target is combined with a lambda type antibody binding to the second target, and vice versa.
  • the resulting co-expression product and the relevant product related impurities are characterized in detail with the intend to select a combination which results in the best profile, especially the ones which show only a low amount of promiscuous binding of one light chain (e.g. L1 , ligh chain 1 , e.g. lambda), towards the wrong heavy chain (e.g. H2, heavy chain 2).
  • An advantage of the method may be that antibody combinations where both light chains exchange the original heavy chain binding partner, which results in a product related impurity of H 1 L2-H2L1 type, can be deselected. This is advantageous, because such product related impurities are not easy to deplete using state of the art purification processes.
  • the procedure how to co-express the individual antibodies and how to analyze the co-expression product is outlined in more detail below.
  • Specific antibodies were used as examples, primarily mAb2 binding to I L- 1 ⁇ , with light chain VK6 and the mAbl binding to I L-18, with light chain VA1.
  • a KiH modification of the Fc-portion of the two antibodies according to Ridgway et al. , (1996) was used. Other antibodies were also tested.
  • bbmAbl is expressed with a single, common cell line ensuring a sufficient overall yield, purity and product quality required for biologic or diagnostics to proceed with clinical development and commercialization.
  • I L-18 is synonym to I L-18 polypeptide, lnterleukin-18 polypeptide, I FN- gamma inducing factor or Interferon-gamma-inducing-factor or INF- ⁇ inducing factor.
  • I L-18 refers to human IL-18, unless another species is indicated. I L-18 is well known to a person skilled in the art, and for example obtainable from MBL® International Corporation under product reference #B001 -5.
  • IL- 18 encompasses both pro-I L-18 (precursor of mature I L-18 prior protease cleavage) and mature I L-18 (post protease cleavage) interchangeably unless it is specified that the pro- or mature form is meant.
  • I L-1 ⁇ or " I L- 1 b" is synonym to I L-1 ⁇ polypeptide and lnterleukin- ⁇ ⁇ polypeptide.
  • IL- ⁇ refers to human I L-1 ⁇ unless another species is indicated.
  • I L-1 ⁇ is well known to a person skilled in the art, and for example obtainable from Sino Biological under product reference #10139-HNAE-5.
  • antibody refers to an intact immunoglobulin or a functional fragment thereof.
  • Naturally occurring antibodies typically comprise a tetramer which is usually composed of at least two heavy (H) chains and at least two light (L) chains.
  • Each heavy chain is comprised of a heavy chain variable region (abbreviated herein as VH) and a heavy chain constant region, usually comprised of three domains (CH1 , CH2 ad CH3).
  • Heavy chains can be of any isotype, including IgG (lgG1 , lgG2, lgG3 and lgG4 subtypes), IgA (lgA1 and lgA2 subtypes), IgM and IgE.
  • Each light chain is comprised of a light chain variable region (abbreviated herein as VL) and a light chain constant region (CL).
  • Light chain includes kappa ( ⁇ ) chains and lambda ( ⁇ ) chains.
  • the heavy and light chain variable region is typically responsible for antigen recognition, whilst the heavy and light chain constant region may mediate the binding of the immunoglobulin to host tissues or factors, including various cells of the immune system (e.g. effector cells) and the first component (Clq) of the classical complement system.
  • the VH and VL regions can be further subdivided into regions of hypervariability, termed complementarity determining regions (CDR), interspersed with regions that are more conserved, termed framework regions (FR).
  • CDR complementarity determining regions
  • Each VH and VL is composed of three CDRs and four FRs arranged from amino-terminus to carboxy-terminus in the following order: FR1 , CDR1 , FR2, CDR2, FR3, CDR3, FR4.
  • the variable regions of the heavy and light chains contain a binding domain that interacts with an antigen.
  • antigen-binding portion of an antibody refers to full length or one or more fragments of an antibody that retain the ability to specifically bind to IL-18 or I L- 1 ⁇ antigen. It has been shown that the antigen- binding function of an antibody can be performed by fragments of a full-length antibody.
  • binding fragments encompassed within the term "antigen-binding portion" of an antibody include a Fab fragment, a monovalent fragment consisting of the VL, VH, CL and CH1 domains; a F(ab)2 fragment, a bivalent fragment comprising two Fab fragments linked by a disulfide bridge at the hinge region; a Fd fragment consisting of the VH and CH1 domains; a Fv fragment consisting of the VL and VH domains of a single arm of an antibody; a dAb fragment (Ward et al., (1989) Nature; 341 :544-546), which consists of a VH domain; and an isolated complementarity determining region (CDR).
  • Fab fragment a monovalent fragment consisting of the VL, VH, CL and CH1 domains
  • F(ab)2 fragment a bivalent fragment comprising two Fab fragments linked by a disulfide bridge at the hinge region
  • a Fd fragment consisting of the VH and CH1
  • the two domains of the Fv fragment, VL and VH are coded for by separate genes, they can be joined, using recombinant methods, by a flexible linker that enables them to be made as a single protein chain in which the VL and VH regions pair to form monovalent molecules (known as single chain Fv (scFv); see e.g. Bird et al., (1988) Science 242:423-426; and Huston et al., (1988) Proc Natl Acad Sc;. 85:5879-5883).
  • single chain Fv single chain Fv
  • Such single chain antibodies are also intended to be encompassed within the term "antigen-binding portion" of an antibody.
  • isolated means throughout this specification, that the immunoglobulin, antibody or polynucleotide, as the case may be, exists in a physical milieu distinct from that in which it may occur in nature.
  • CDR complementarity determining regions
  • VH heavy chain variable domain
  • HCDR2 heavy chain variable domain
  • HCDR3 CDR amino acid residues in the light chain variable domain
  • LCDR1 CDR amino acid residues in the light chain variable domain
  • LCDR3 Under Chothia the CDR amino acids in the VH are numbered 26-32 (HCDR1), 52-56 (HCDR2), and 95-102 (HCDR3); and the amino acid residues in VL are numbered 26-32 (LCDR1), 50-52 (LCDR2), and 91-96 (LCDR3).
  • the CDRs consist of amino acid residues 26-35 (HCDR1), 50- 65 (HCDR2), and 95-102 (HCDR3) in human VH and amino acid residues 24-34
  • LCDR1 LCDR1
  • 50-56 LCDR2
  • LCDR3 LCDR3
  • the CDR amino acid residues in the VH are numbered approximately 26-35 (CDR1), 51-57 (CDR2) and 93-102 (CDR3)
  • the CDR amino acid residues in the VL are numbered approximately 27-32 (CDR1), 50-52 (CDR2), and 89-97 (CDR3) (numbering according to "Kabat").
  • CDR3 number of the CDR regions of an antibody can be determined using the program IMGT/DomainGap Align.
  • H- CDR1 , H-CDR2 and H-CDR3 CDR regions in the heavy chain are typically referred to as H- CDR1 , H-CDR2 and H-CDR3 and in the light chain as L-CDR1 , LCDR2 and L-CDR3. They are numbered sequentially in the direction from the amino terminus to the carboxy terminus.
  • monoclonal antibody or “monoclonal antibody composition” as used herein refer to a preparation of antibody molecules of single molecular composition.
  • a monoclonal antibody composition displays a single binding specificity and affinity for a particular epitope.
  • human antibody is intended to include antibodies having variable regions in which both the framework and CDR regions are derived from sequences of human origin. Furthermore, if the antibody contains a constant region, the constant region also is derived from such human sequences, e.g. human germline sequences, or mutated versions of human germline sequences or antibody containing consensus framework sequences derived from human framework sequences analysis, for example, as described in Knappik, et al., (2000) J Mol Biol; 296:57-86).
  • human antibodies of the invention may include amino acid residues not encoded by human sequences (e.g. mutations introduced by random or site-specific mutagenesis in vitro or by somatic mutation in vivo).
  • human antibody as used herein, is not intended to include antibodies in which CDR sequences derived from the germline of another mammalian species, such as a mouse, have been grafted onto human framework sequences.
  • human monoclonal antibody refers to antibodies displaying a single binding specificity which have variable regions in which both the framework and CDR regions are derived from human sequences.
  • recombinant human antibody includes all human antibodies that are prepared, expressed, created or isolated by recombinant means, such as antibodies isolated from an animal (e.g. a mouse) that is transgenic or transchromosomal for human immunoglobulin genes or a hybridoma prepared therefrom, antibodies isolated from a host cell transformed to express the human antibody, e.g. from a transfectoma, antibodies isolated from a recombinant, combinatorial human antibody library, and antibodies prepared, expressed, created or isolated by any other means that involve splicing of all or a portion of a human immunoglobulin gene.
  • an animal e.g. a mouse
  • transgenic or transchromosomal for human immunoglobulin genes or a hybridoma prepared therefrom antibodies isolated from a host cell transformed to express the human antibody, e.g. from a transfectoma
  • antibodies isolated from a recombinant e.g. from a recombinant, combinatorial human antibody library
  • Such recombinant human antibodies have variable regions in which the framework and CDR regions are derived from human germline immunoglobulin sequences.
  • such recombinant human antibodies can be subjected to in vitro mutagenesis (or, when an animal transgenic for human Ig sequences is used, in vivo somatic mutagenesis) and thus the amino acid sequences of the VH and VL regions of the recombinant antibodies are sequences that, while derived from and related to human germline VH and VL sequences, may not naturally exist within the human antibody germline repertoire in vivo.
  • an antibody recognizing an antigen and “an antibody specific for an antigen” are used interchangeably herein with the term “an antibody which binds specifically to an antigen”.
  • a binding molecule that "specifically binds to IL-18” is intended to refer to a binding molecule that binds to human IL-18 with a K D of a 100nM or less, 10nM or less, 1 nM or less.
  • a binding molecule that "specifically binds to I L- 1 ⁇ " is intended to refer to a binding molecule that binds to human I L-1 ⁇ with a K D of a 100nM or less, 10nM or less, 1 nM or less.
  • a binding molecule that "cross-reacts with an antigen other than IL-18 is intended to refer to a binding molecule that binds that antigen with a KD of a 100nM or less, 10nM or less, 1 nM or less.
  • a binding molecule that "cross-reacts with an antigen other than IL- 1 ⁇ is intended to refer to a binding molecule that binds that antigen with a KD of a 100nM or less, 10nM or less, 1 nM or less.
  • a binding molecule that "does not cross-react with a particular antigen" is intended to refer to a binding molecule that exhibits essentially undetectable binding against these proteins in standard binding assays.
  • an IL-18 antagonist would be a binding molecule inhibiting the signalling activity in the presence of IL-18 in a human cell assay such as IL-18 dependent Interferon-gamma (IFN- ⁇ ) production assay in human blood cells.
  • IFN- ⁇ Interferon-gamma
  • bivalent bispecific antibody or bivalent bispecific antibodies refer to antibodies binding to two different targets, such as IL-18 and I L- 1 .
  • the bispecific antibodies are "hetero-dimers", which means that one part comes from first antibody, specific for a first target, and another part comes from a second antibody, specific for a second target.
  • a “hetero-dimerization modification” is a modification to one or both parts of the antibodies forming the hetero-dimeric bispecific antibody, intended to facilitate such formation.
  • hetero-dimerization modifications of the Fc domains of two lgG1 parts of antibodies intended to form a bispecific is a "knob" with a bulky amino acid (aa) side chain (S354C, T366W) in the first heavy chain and a "hole” with small aa side chains (Y349C, T366S, L368A, Y407V) were introduced in the second heavy chain as well as an additional disulfide bridge in the CH3 region connecting both heavy chains (Merchant et al., Nat. Biotechnol., 16:677-681 (1998), page 678, table 1).
  • aa side chain S354C, T366W
  • Y349C, T366S, L368A, Y407V small aa side chains
  • an antibody with "no agonistic activity” is intended to refer to a binding molecule that does not significantly increase target dependent signalling activity in the absence and/or presence of the target in a cell-based assay, such as in case of IL- 18, does not significantly increase IL-18 dependent signalling activity in the absence and/or presence of IL-18 human blood cells IFN- ⁇ production assay.
  • a cell-based assay such as in case of IL- 18, does not significantly increase IL-18 dependent signalling activity in the absence and/or presence of IL-18 human blood cells IFN- ⁇ production assay.
  • K asS oc or "Kg”, as used herein, is intended to refer to the association rate of a particular binding molecule -antigen interaction
  • K d i S or "K d ,” as used herein, is intended to refer to the dissociation rate of a particular binding molecule -antigen interaction
  • KD is intended to refer to the dissociation constant, which is obtained from the ratio of Kd to Ka (i.e. Kd/K a ) and is expressed as a molar concentration (M).
  • KD values for antibodies can be determined using methods well established in the art. A method for determining the KD of an antibody is by using surface plasmon resonance, such as a Biacore® system.
  • affinity refers to the strength of interaction between binding molecule and antigen at single antigenic sites.
  • high affinity for an antibody refers to an antibody having a KD of 1 nM or less for a target antigen.
  • the term “subject” includes any human or non-human animal.
  • non-human animal includes all vertebrates, e.g. mammals and non- mammals, such as non-human primates, sheep, dogs, cats, horses, cows, chickens, amphibians, reptiles, etc.
  • the term, "optimized nucleotide sequence” means that the nucleotide sequence has been altered to encode an amino acid sequence using codons that are preferred in the production cell or organism, generally a eukaryotic cell, for example, a cell of Pichia pastoris, a Chinese Hamster Ovary cell (CHO) or a human cell.
  • the optimized nucleotide sequence is engineered to retain completely the amino acid sequence originally encoded by the starting nucleotide sequence, which is also known as the "parental" sequence.
  • the optimized sequences herein have been engineered to have codons that are preferred in CHO mammalian cells; however optimized expression of these sequences in other eukaryotic cells is also envisioned herein.
  • identity refers to the similarity between at least two different sequences. This identity can be expressed as a percent identity and determined by standard alignment algorithms, for example, the Basic Local Alignment Tool (BLAST) (Altshul et al., (1990) J Mol Biol; 215:403-410); the algorithm of Needleman et al., (1970) J Mol Biol; 48:444-453 or the algorithm of Meyers et al., (1988) Comput Appl Biosci; 4: 11-17).
  • BLAST Basic Local Alignment Tool
  • a set of parameters may be the Blosum 62 scoring matrix with a gap penalty of 12, a gap extend penalty of 4, and a frameshift gap penalty of 5.
  • the percent identity between two amino acid or nucleotide sequences can also be determined using the algorithm of E. Meyers and W. Miller, (1989) CABIOS; 4(1): 1-17) which has been incorporated into the ALIGN program (version 2.0), using a PAM 120 weight residue table, a gap length penalty of 12 and a gap penalty of 4. The percent identity is usually calculated by comparing sequences of similar length.
  • immune response refers to the action of, for example, lymphocytes, antigen presenting cells, phagocytic cells, granulocytes, and soluble macromolecules produced by the above cells or the liver (including antibodies, cytokines, and
  • a “signal transduction pathway” or “signaling activity” refers to a biochemical causal relationship generally initiated by a protein-protein interaction such as binding of a growth factor to a receptor, resulting in transmission of a signal from one portion of a cell to another portion of a cell.
  • the transmission involves specific phosphorylation of one or more tyrosine, serine, or threonine residues on one or more proteins in the series of reactions causing signal transduction.
  • Penultimate processes typically include nuclear events, resulting in a change in gene expression.
  • neutralises and grammatical variations thereof means throughout this specification, that the biological activity of the target is reduced either totally or partially in the presence of the binding protein or antibody, as the case may be.
  • nucleic acid or “polynucleotide” refers to deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) and polymers thereof in either single- or double-stranded form. Unless specifically limited, the term encompasses nucleic acids containing known analogues of natural nucleotides that have similar binding properties as the reference nucleic acid and are metabolized in a manner similar to naturally occurring nucleotides. Unless otherwise indicated, a particular nucleic acid sequence also implicitly
  • degenerate codon substitutions may be achieved by generating sequences in which the third position of one or more selected (or all) codons is substituted with mixed-base and/or deoxyinosine residues (Batzer et al., Nucleic Acid Res. 19:5081 (1991); Ohtsuka et al., J. Biol. Chem. 260:2605-2608 (1985); and Rossolini et al., Mol. Cell. Probes 8:91-98 (1994))
  • nucleotide in the "polynucleotide” or “nucleic acid” may comprise
  • modifications including base modifications such as bromouridine and inosine derivatives, ribose modification such as phosphorothioate, phosphorodithioate, phosphoroselenoate, phosphorodiselenoate, phosphoroanilothioate, phosphoraniladate and phosphoroamidate.
  • vector means any molecule or entity (e.g. nucleic acid, plasmid, bacteriophage or virus) that is suitable for transformation or transfection of a host cell and contains nucleic acid sequences that direct and/or control (in conjunction with the host cell) expression of one or more heterologous coding regions operatively linked thereto.
  • entity e.g. nucleic acid, plasmid, bacteriophage or virus
  • co-expression means that different polypeptides are expressed together in a single host cell, common for all the polypeptides.
  • Co-expression of a bispecific antibody means that the different parts forming the functional bispecific antibody are expressed in a single, common, host cell. Co-expression may be achieved by incorporating several expression vectors in the expression host cell, such as one for each of the halves of a bispecific antibody, or by incorporating one expression vector encoding all parts of the bispecific antibody.
  • mismatching means that different parts of an intended protein complex, such as a bispecific antibody, do not complex bind as intended, which means that the protein complex does not look or behave as intended. Examples of mismatching in the context of a bispecific antibody are shown in Figure 4.
  • a “conservative variant” of a sequence encoding a binding molecule, an antibody or a fragment thereof refers to a sequence comprising conservative amino acid modifications.
  • “Conservative amino acid modifications” are intended to refer to amino acid modifications that do not significantly affect or alter the binding characteristics of the antibody containing the amino acid sequence. Such conservative modifications include amino acid substitutions, additions and deletions. Conservative amino acid substitutions are ones in which the amino acid residue is replaced with an amino acid residue having a similar side chain. Families of amino acid residues having similar side chains have been defined in the art. These families include amino acids with basic side chains (e.g. lysine, arginine, histidine), acidic side chains (e.g.
  • aspartic acid glutamic acid
  • uncharged polar side chains e.g. glycine, asparagine, glutamine, serine, threonine, tyrosine, cysteine, tryptophan
  • nonpolar side chains e.g. alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine
  • beta-branched side chains e.g. threonine, valine, isoleucine
  • aromatic side chains e.g. tyrosine, phenylalanine, tryptophan, histidine
  • Modifications can be introduced into a binding protein of the invention by standard techniques known in the art, such as site-directed mutagenesis and PCR-mediated mutagenesis.
  • Conservative amino acid substitution can also encompass non-naturally occurring amino acid residues which are typically incorporated by chemical peptide synthesis rather than by synthesis in biological systems.
  • Non-naturally occurring amino acids include, but are not limited to, peptidomimetic, reversed or inverted forms of amino acid moieties.
  • epitope is the part of an antigen that is recognized by the immune system , such as an antibody or a fragment thereof.
  • the term “epitope” is used interchangeably for both conformational epitopes and linear epitopes.
  • a conformational epitope is composed of discontinuous sections of the antigen's amino acid sequence, whilst a linear epitope is formed by a continuous sequence of amino acids from the antigen.
  • a human antibody or a fragment thereof comprises heavy or light chain variable regions or full length heavy or light chains that are "the product of” or “derived from” a particular germline sequence if the variable regions or full length chains of the antibody are obtained from a system that uses human germline immunoglobulin genes.
  • Such systems include immunizing a transgenic mouse carrying human immunoglobulin genes with the antigen of interest or screening a human immunoglobulin gene library displayed on phage with the antigen of interest.
  • a human antibody or fragment thereof that is "the product of or "derived from” a human germline immunoglobulin sequence can be identified as such by comparing the amino acid sequence of the human antibody to the amino acid sequences of human germline immunoglobulins and selecting the human germline immunoglobulin sequence that is closest in sequence (i.e., greatest % identity) to the sequence of the human antibody.
  • a human antibody that is "the product of” or “derived from” a particular human germline immunoglobulin sequence may contain amino acid differences as compared to the germline sequence, due to, for example, naturally occurring somatic mutations or intentional introduction of site-directed mutation.
  • a selected human antibody typically is at least 90% identical in amino acids sequence to an amino acid sequence encoded by a human germline immunoglobulin gene and contains amino acid residues that identify the human antibody as being human when compared to the germline immunoglobulin amino acid sequences of other species (e.g. murine germline sequences).
  • a human antibody may be at least 60%, 70%, 80%, 90%, or at least 95%, or even at least 96%, 97%, 98%, or 99% identical in amino acid sequence to the amino acid sequence encoded by the germline immunoglobulin gene.
  • a human antibody derived from a particular human germline sequence will display no more than 10 amino acid differences from the amino acid sequence encoded by the human germline immunoglobulin gene.
  • the human antibody may display no more than 5, or even no more than 4, 3, 2, or 1 amino acid difference from the amino acid sequence encoded by the germline immunoglobulin gene.
  • Human antibodies may be produced by a number of methods known to those of skill in the art. Human antibodies can be made by the hybridoma method using human myeloma or mouse-human heteromyeloma cells lines (Kozbor, J Immunol; (1984) 133:3001 ; Brön, Monoclonal Isolated Antibody Production Techniques and
  • mice Several strains of transgenic mice are now available wherein their mouse immunoglobulin loci has been replaced with human immunoglobulin gene segments (Tomizuka K, (2000) Proc Natl Acad Sci , 97:722-727; Fishwild DM (1996) Nature Biotechnol 14:845-851 ; Mendez MJ, (1997) Nature Genetics 15: 146-156). Upon antigen challenge such mice are capable of producing a repertoire of human antibodies from which antibodies of interest can be selected.
  • Phage display technology can be used to produce human antibodies and fragments thereof, (McCafferty; (1990) Nature, 348:552-553 and Griffiths AD et al (1994) EMBO 13:3245-3260).
  • isolated antibody variable domain genes are cloned in frame into either a major or minor coat of protein gene of a filamentous bacteriophage such as M13 or fd and displayed (usually with the aid of a helper phage) as function isolated antibody fragments on the surface of the phage particle. Selections based on the function properties of the isolated antibody result in selection of the gene encoding the isolated antibody exhibiting these properties.
  • the phage display technique can be used to select antigen specific antibodies from libraries made from human B cells taken from individuals afflicted with a disease or disorder or alternatively from unimmunized human donors (Marks; J Mol Bio (1991) 222:581-591 ,). Where an intact human isolated antibody is desired comprising an Fc domain it is necessary redone the phage displayed derived fragment into a mammalian expression vectors comprising the desired constant regions and establishing stable expressing cell lines.
  • the technique of affinity maturation may be used to provide binding affinity wherein the affinity of the primary human isolated antibody is improved by sequentially replacing the H and L chain variable regions with naturally occurring variants and selecting on the basis of improved binding affinities. Variants of this technique such as 'epitope imprinting' are now also available (WO 93/06213; Waterhouse; Nucl Acids Res (1993) 21 :2265-2266).
  • purified bispecific antibody when used in the context of purified bispecific antibody relates to purity and identity of different bispecific antibody combinations and constructs after co- expression in selected cells under conditions wherein the cells express the bispecific antibody and after protein-A purification using an intact UPLC-MS mass screening approach.
  • Pure or purity refers to the relative quantify of the formed hetero-and homodimer bbmAbs. Using the method of the invention correctly formed heterodimeric bbmAbl and bbmAb2 could be observed with a relative purity of over 85% based on intact mass signal intensity.
  • I L-18 antibodies or antigen-binding fragments thereof used in the disclosed methods are human antibodies.
  • mAbl the amino acid sequences of the hypervariable regions of a specific IL-18 antibody, called mAbl , based on the Kabat definition and the Chothia definition, as well as the V L and V H domains and full heavy and light chains are provided in Table 1 , below.
  • the IL-18 antibody or antigen-binding fragment thereof comprises at least one immunoglobulin heavy chain variable domain (VH) comprising hypervariable regions CDR1 , CDR2 and CDR3, said CDR1 having the amino acid sequence SEQ ID NO: 1 , said CDR2 having the amino acid sequence SEQ ID NO:2, and said CDR3 having the amino acid sequence SEQ ID NO:3.
  • VH immunoglobulin heavy chain variable domain
  • the IL- 18 antibody or antigen-binding fragment thereof comprises at least one immunoglobulin heavy chain variable domain (V H ) comprising hypervariable regions CDR1 , CDR2 and CDR3, said CDR1 having the amino acid sequence SEQ ID NO:4, said CDR2 having the amino acid sequence SEQ ID NO:5, and said CDR3 having the amino acid sequence SEQ ID NO:6.
  • V H immunoglobulin heavy chain variable domain
  • the IL-18 antibody or antigen-binding fragment thereof comprises at least one immunoglobulin light chain variable domain (VL) comprising hypervariable regions CDR1 , CDR2 and CDR3, said CDR1 having the amino acid sequence SEQ ID NO: 1 1 , said CDR2 having the amino acid sequence SEQ ID NO: 12 and said CDR3 having the amino acid sequence SEQ ID NO:13.
  • VL immunoglobulin light chain variable domain
  • the IL-18 antibody or antigen-binding fragment thereof comprises at least one immunoglobulin light chain variable domain (VL) comprising hypervariable regions CDR1 , CDR2 and CDR3, said CDR1 having the amino acid sequence SEQ ID NO: 1 1 , said CDR2 having the amino acid sequence SEQ ID NO: 12 and said CDR3 having the amino acid sequence SEQ ID NO:13.
  • VL immunoglobulin light chain variable domain
  • the IL-18 antibody or antigen-binding fragment thereof comprises at least one immunoglobulin V H domain and at least one immunoglobulin V L domain, wherein: a) the immunoglobulin V H domain comprises (e.g.
  • the immunoglobulin VL domain comprises (e.g.
  • the IL-18 antibody or antigen-binding fragment thereof comprises: a) an immunoglobulin heavy chain variable domain (VH) comprising the amino acid sequence set forth as SEQ ID NO:7; b) an immunoglobulin light chain variable domain (VL) comprising the amino acid sequence set forth as SEQ ID NO: 17; c) an immunoglobulin V H domain comprising the amino acid sequence set forth as SEQ ID NO:7 and an immunoglobulin V L domain comprising the amino acid sequence set forth as SEQ ID NO:17; d) an immunoglobulin V H domain comprising the hypervariable regions set forth as SEQ ID NO: 1 , SEQ ID NO:2, and SEQ ID NO:3; e) an immunoglobulin heavy chain variable domain (VH) comprising the amino acid sequence set forth as SEQ ID NO:7; b) an immunoglobulin light chain variable domain (VL) comprising the amino acid sequence set forth as SEQ ID NO: 17; c) an immunoglobulin V H domain comprising the amino acid sequence
  • immunoglobulin V L domain comprising the hypervariable regions set forth as SEQ ID NO: 11 , SEQ ID NO: 12 and SEQ ID NO: 13; f) an immunoglobulin V H domain comprising the hypervariable regions set forth as SEQ ID NO:4, SEQ ID NO:5 and SEQ ID NO:6; g) an immunoglobulin VL domain comprising the hypervariable regions set forth as SEQ ID NO: 14, SEQ ID NO: 15 and SEQ ID NO: 16; h) an immunoglobulin VH domain comprising the hypervariable regions set forth as SEQ ID NO: 1 , SEQ ID NO:2, and SEQ ID NO:3 and an immunoglobulin VL domain comprising the hypervariable regions set forth as SEQ ID NO:1 1 , SEQ ID NO:12 and SEQ ID NO: 13; i) an immunoglobulin V H domain comprising the hypervariable regions set forth as SEQ ID NO:4, SEQ ID NO:5, and SEQ ID NO:6 and an immunoglobulin VL domain
  • the IL-18 antibody or antigen-binding fragment thereof comprises the three CDRs of SEQ ID NO:7. In other embodiments, the IL-18 antibody or antigen-binding fragment thereof comprises the three CDRs of SEQ ID NO: 17. In other embodiments, the IL-18 antibody or antigen-binding fragment thereof comprises the three CDRs of SEQ ID NO:7 and the three CDRs of SEQ ID NO: 17. In some embodiments, the IL-18 antibody or antigen-binding fragment thereof comprises the three CDRs of SEQ ID NO:9. In other embodiments, IL-18 antibody or antigen- binding fragment thereof comprises the three CDRs of SEQ ID NO: 19. In other embodiments, the IL-18 antibody or antigen-binding fragment thereof comprises the three CDRs of SEQ ID NO:9 and the three CDRs of SEQ ID NO: 19.
  • the IL-18 antibody or antigen-binding fragment thereof is selected from a human IL-18 antibody that comprises at least: a) an
  • immunoglobulin heavy chain or fragment thereof which comprises a variable domain comprising, in sequence, the hypervariable regions CDR1 , CDR2 and CDR3 and the constant part or fragment thereof of a human heavy chain; said CDR1 having the amino acid sequence SEQ ID NO: 1 , said CDR2 having the amino acid sequence SEQ ID NO:2, and said CDR3 having the amino acid sequence SEQ ID NO:3; and b) an
  • immunoglobulin light chain or fragment thereof which comprises a variable domain comprising, in sequence, the hypervariable regions CDR1 , CDR2, and CDR3 and the constant part or fragment thereof of a human light chain, said CDR1 having the amino acid sequence SEQ ID NO:1 1 , said CDR2 having the amino acid sequence SEQ ID NO: 12, and said CDR3 having the amino acid sequence SEQ ID NO:13.
  • the IL-18 antibody or antigen-binding fragment thereof is selected from a human IL-18 antibody that comprises at least: a) an
  • immunoglobulin heavy chain or fragment thereof which comprises a variable domain comprising, in sequence, the hypervariable regions CDR1 , CDR2 and CDR3 and the constant part or fragment thereof of a human heavy chain; said CDR1 having the amino acid sequence SEQ ID NO:4, said CDR2 having the amino acid sequence SEQ ID NO:5 and said CDR3 having the amino acid sequence SEQ ID NO:6; and b) an
  • immunoglobulin light chain or fragment thereof which comprises a variable domain comprising, in sequence, the hypervariable regions CDR1 , CDR2, and CDR3 and the constant part or fragment thereof of a human light chain, said CDR1 having the amino acid sequence SEQ ID NO:14, said CDR2 having the amino acid sequence SEQ ID NO: 15, and said CDR3 having the amino acid sequence SEQ ID NO:16.
  • the IL-18 antibody or antigen-binding fragment thereof is selected from a single chain antibody or antigen-binding fragment thereof that comprises an antigen-binding site comprising: a) a first domain comprising, in sequence, the hypervariable regions CDR1 , CDR2 and CDR3, said CDR1 having the amino acid sequence SEQ ID NO: 1 , said CDR2 having the amino acid sequence SEQ ID NO:2, and said CDR3 having the amino acid sequence SEQ ID NO:3; and b) a second domain comprising, in sequence, the hypervariable regions CDR1 , CDR2 and CDR3, said CDR1 having the amino acid sequence SEQ ID NO: 11 , said CDR2 having the amino acid sequence SEQ ID NO: 12, and said CDR3 having the amino acid sequence SEQ ID NO: 13; and c) a peptide linker which is bound either to the N-terminal extremity of the first domain and to the C-terminal extremity of the second domain or to the C-terminal extremity of the
  • the IL-18 antibody or antigen-binding fragment thereof is selected from a single chain antibody or antigen-binding fragment thereof that comprises an antigen-binding site comprising: a) a first domain comprising, in sequence, the hypervariable regions CDR1 , CDR2 and CDR3, said CDR1 having the amino acid sequence SEQ ID NO:4, said CDR2 having the amino acid sequence SEQ ID NO:5, and said CDR3 having the amino acid sequence SEQ ID NO:6; and b) a second domain comprising, in sequence, the hypervariable regions CDR1 , CDR2 and CDR3, said CDR1 having the amino acid sequence SEQ ID NO: 14, said CDR2 having the amino acid sequence SEQ ID NO: 15, and said CDR3 having the amino acid sequence SEQ ID NO: 16; and c) a peptide linker which is bound either to the N-terminal extremity of the first domain and to the C-terminal extremity of the second domain or to the C-termin
  • V H or V L domain of an IL-18 antibody or antigen-binding fragment thereof used in the disclosed methods may have V H and/or V L domains that are substantially identical to the V H or V L domains set forth in SEQ ID NO:7 and 17.
  • a human IL-18 antibody disclosed herein may comprise a heavy chain that is substantially identical to that set forth as SEQ ID NO:9 and/or a light chain that is substantially identical to that set forth as SEQ ID NO: 19.
  • a human IL-18 antibody disclosed herein may comprise a heavy chain that comprises SEQ ID NO:9 and a light chain that comprises SEQ ID NO: 19.
  • a human IL-18 antibody disclosed herein may comprise: a) one heavy chain, comprising a variable domain having an amino acid sequence substantially identical to that shown in SEQ ID NO:7 and the constant part of a human heavy chain; and b) one light chain, comprising a variable domain having an amino acid sequence substantially identical to that shown in SEQ ID NO: 17 and the constant part of a human light chain.
  • IL-18 antagonists e.g. antibodies
  • preferred IL-18 antagonists for use in the disclosed methods, kits and regimens are those set forth in US Patent No: 9,376,489, which is incorporated by reference herein in its entirety. 3.
  • I L- 1 ⁇ antibodies or antigen-binding fragments thereof used in the disclosed methods are human antibodies.
  • amino acid sequences of the hypervariable regions of a specific I L- 1 ⁇ antibody are provided in Table 2, below.
  • the I L-1 ⁇ antibody or antigen-binding fragment thereof comprises at least one immunoglobulin heavy chain variable domain (V H ) comprising hypervariable regions CDR1 , CDR2 and CDR3, said CDR1 having the amino acid sequence SEQ ID NO:21 , said CDR2 having the amino acid sequence SEQ ID NO:22, and said CDR3 having the amino acid sequence SEQ ID NO:23.
  • V H immunoglobulin heavy chain variable domain
  • the I L-1 ⁇ antibody or antigen-binding fragment thereof comprises at least one immunoglobulin heavy chain variable domain (V H ) comprising hypervariable regions CDR1 , CDR2 and CDR3, said CDR1 having the amino acid sequence SEQ ID NO:21 , said CDR2 having the amino acid sequence SEQ ID NO:22, and said CDR3 having the amino acid sequence SEQ ID NO:23.
  • the I L-1 ⁇ antibody or antigen-binding fragment thereof comprises at least one
  • immunoglobulin heavy chain variable domain comprising hypervariable regions CDR1 , CDR2 and CDR3, said CDR1 having the amino acid sequence SEQ ID NO:24, said CDR2 having the amino acid sequence SEQ ID NO:25, and said CDR3 having the amino acid sequence SEQ ID NO:26.
  • the I L-1 ⁇ antibody or antigen-binding fragment thereof comprises at least one immunoglobulin light chain variable domain (VL) comprising hypervariable regions CDR1 , CDR2 and CDR3, said CDR1 having the amino acid sequence SEQ ID NO:31 , said CDR2 having the amino acid sequence SEQ ID NO:32 and said CDR3 having the amino acid sequence SEQ ID NO:33.
  • VL immunoglobulin light chain variable domain
  • the I L-1 ⁇ antibody or antigen-binding fragment thereof comprises at least one immunoglobulin light chain variable domain (VL) comprising hypervariable regions CDR1 , CDR2 and CDR3, said CDR1 having the amino acid sequence SEQ ID NO:31 , said CDR2 having the amino acid sequence SEQ ID NO:32 and said CDR3 having the amino acid sequence SEQ ID NO:33.
  • VL immunoglobulin light chain variable domain
  • VL immunoglobulin light chain variable domain
  • the I L-1 ⁇ antibody or antigen-binding fragment thereof comprises at least one immunoglobulin V H domain and at least one immunoglobulin V L domain
  • the immunoglobulin V H domain comprises (e.g. in sequence): i) hypervariable regions CDR1 , CDR2 and CDR3, said CDR1 having the amino acid sequence SEQ ID NO:21 , said CDR2 having the amino acid sequence SEQ ID NO:22, and said CDR3 having the amino acid sequence SEQ ID NO:23; or ii) hypervariable regions CDR1 , CDR2 and CDR3, said CDR1 having the amino acid sequence SEQ ID NO:24, said CDR2 having the amino acid sequence SEQ ID NO:25, and said CDR3 having the amino acid sequence SEQ ID NO:26; and b) the immunoglobulin VL domain comprises (e.g.
  • the IL- ⁇ antibody or antigen-binding fragment thereof comprises: a) an immunoglobulin heavy chain variable domain (V H ) comprising the amino acid sequence set forth as SEQ ID NO:27; b) an immunoglobulin light chain variable domain (V L ) comprising the amino acid sequence set forth as SEQ ID NO:37; c) an immunoglobulin V H domain comprising the amino acid sequence set forth as SEQ ID NO:27 and an immunoglobulin V L domain comprising the amino acid sequence set forth as SEQ ID NO:37; d) an immunoglobulin V H domain comprising the hypervariable regions set forth as SEQ ID NO:21 , SEQ ID NO:22, and SEQ ID NO:23; e) an immunoglobulin V H domain comprising the hypervariable regions set forth as SEQ ID NO:21 , SEQ ID NO:22, and SEQ ID NO:23; e) an immunoglobulin V H domain comprising the hypervariable regions set forth as SEQ ID NO:21 , SEQ ID NO
  • immunoglobulin VL domain comprising the hypervariable regions set forth as SEQ ID NO:31 , SEQ ID NO:32 and SEQ ID NO:33; f) an immunoglobulin V H domain comprising the hypervariable regions set forth as SEQ ID NO:24, SEQ ID NO:25 and SEQ ID NO:26; g) an immunoglobulin VL domain comprising the hypervariable regions set forth as SEQ ID NO:34, SEQ ID NO:35 and SEQ ID NO:36; h) an immunoglobulin V H domain comprising the hypervariable regions set forth as SEQ ID NO:21 , SEQ ID NO:22, and SEQ ID NO:23 and an immunoglobulin VL domain comprising the hypervariable regions set forth as SEQ ID NO:31 , SEQ ID NO:32 and SEQ ID NO:33; i) an immunoglobulin V H domain comprising the hypervariable regions set forth as SEQ ID NO:24, SEQ ID NO:25, and SEQ ID NO:26 and an immunoglobul
  • the I L-1 ⁇ antibody or antigen-binding fragment thereof (e.g. mAb2) comprises the three CDRs of SEQ ID NO:37. In other embodiments, the I L-1 ⁇ antibody or antigen-binding fragment thereof comprises the three CDRs of SEQ ID NO:27. In other embodiments, the I L- 1 ⁇ antibody or antigen-binding fragment thereof comprises the three CDRs of SEQ ID NO:37 and the three CDRs of SEQ ID NO:27. In some embodiments, the I L-1 ⁇ antibody or antigen-binding fragment thereof comprises the three CDRs of SEQ ID NO:39. In other embodiments, I L-1 ⁇ antibody or antigen- binding fragment thereof comprises the three CDRs of SEQ ID NO:29. In other embodiments, the I L-1 ⁇ antibody or antigen-binding fragment thereof comprises the three CDRs of SEQ ID NO:39 and the three CDRs of SEQ ID NO:29.
  • the I L-1 ⁇ antibody or antigen-binding fragment thereof is selected from a human I L-1 ⁇ antibody that comprises at least: a) an
  • immunoglobulin heavy chain or fragment thereof which comprises a variable domain comprising, in sequence, the hypervariable regions CDR1 , CDR2 and CDR3 and the constant part or fragment thereof of a human heavy chain; said CDR1 having the amino acid sequence SEQ ID NO:21 , said CDR2 having the amino acid sequence SEQ ID NO:22, and said CDR3 having the amino acid sequence SEQ ID NO:23; and b) an immunoglobulin light chain or fragment thereof which comprises a variable domain comprising, in sequence, the hypervariable regions CDR1 , CDR2, and CDR3 and the constant part or fragment thereof of a human light chain, said CDR1 having the amino acid sequence SEQ ID NO:31 , said CDR2 having the amino acid sequence SEQ ID NO:32, and said CDR3 having the amino acid sequence SEQ ID NO:33.
  • the I L-1 ⁇ antibody or antigen-binding fragment thereof is selected from a human I L-1 ⁇ antibody that comprises at least: a) an immunoglobulin heavy chain or fragment thereof which comprises a variable domain comprising, in sequence, the hypervariable regions CDR1 , CDR2 and CDR3 and the constant part or fragment thereof of a human heavy chain; said CDR1 having the amino acid sequence SEQ ID NO:24, said CDR2 having the amino acid sequence SEQ ID NO:25 and said CDR3 having the amino acid sequence SEQ ID NO:26; and b) an immunoglobulin light chain or fragment thereof which comprises a variable domain comprising, in sequence, the hypervariable regions CDR1 , CDR2, and CDR3 and the constant part or fragment thereof of a human light chain, said CDR1 having the amino acid sequence SEQ ID NO:34, said CDR2 having the amino acid sequence SEQ ID NO:35, and said CDR3 having the amino acid sequence SEQ ID NO:36.
  • a human I L-1 ⁇ antibody that
  • the I L-1 ⁇ antibody or antigen-binding fragment thereof is selected from a single chain antibody or antigen-binding fragment thereof that comprises an antigen-binding site comprising: a) a first domain comprising, in sequence, the hypervariable regions CDR1 , CDR2 and CDR3, said CDR1 having the amino acid sequence SEQ ID NO:21 , said CDR2 having the amino acid sequence SEQ ID NO:22, and said CDR3 having the amino acid sequence SEQ ID NO:23; and b) a second domain comprising, in sequence, the hypervariable regions CDR1 , CDR2 and CDR3, said CDR1 having the amino acid sequence SEQ ID NO:31 , said CDR2 having the amino acid sequence SEQ ID NO:32, and said CDR3 having the amino acid sequence SEQ ID NO:33; and c) a peptide linker which is bound either to the N-terminal extremity of the first domain and to the C-terminal extremity of the second domain or to the C-terminal
  • the I L-1 ⁇ antibody or antigen-binding fragment thereof is selected from a single chain antibody or antigen-binding fragment thereof that comprises an antigen-binding site comprising: a) a first domain comprising, in sequence, the hypervariable regions CDR1 , CDR2 and CDR3, said CDR1 having the amino acid sequence SEQ ID NO:24, said CDR2 having the amino acid sequence SEQ ID NO:25, and said CDR3 having the amino acid sequence SEQ ID NO:26; and b) a second domain comprising, in sequence, the hypervariable regions CDR1 , CDR2 and CDR3, said CDR1 having the amino acid sequence SEQ ID NO:34, said CDR2 having the amino acid sequence SEQ ID NO:35, and said CDR3 having the amino acid sequence SEQ ID NO:36; and c) a peptide linker which is bound either to the N-terminal extremity of the first domain and to the C-terminal extremity of the second domain
  • the V H or V L domain of an I L- 1 ⁇ antibody or antigen-binding fragment thereof used in the disclosed methods may have VH and/or VL domains that are substantially identical to the V H or V L domains set forth in SEQ ID NO:27 and 37.
  • a human IL-1 ⁇ antibody disclosed herein may comprise a heavy chain that is substantially identical to that set forth as SEQ ID NO:29 and/or a light chain that is substantially identical to that set forth as SEQ ID NO:39.
  • a human I L-1 ⁇ antibody disclosed herein may comprise a heavy chain that comprises SEQ ID NO:29 and a light chain that comprises SEQ ID NO:39.
  • a human I L- 1 ⁇ antibody disclosed herein may comprise: a) one heavy chain, comprising a variable domain having an amino acid sequence substantially identical to that shown in SEQ ID NO:27 and the constant part of a human heavy chain; and b) one light chain, comprising a variable domain having an amino acid sequence substantially identical to that shown in SEQ ID NO:37 and the constant part of a human light chain.
  • I L-1 ⁇ antagonists e.g. antibodies
  • preferred I L-1 ⁇ antagonists for use in the disclosed methods, kits and regimens are those set forth in US Patent Nos: 7,446, 175 or 7,993,878 or 8,273,350, which are incorporated by reference herein in their entirety.
  • antibodies of the invention may be engineered to include modifications within the Fc region, typically to alter one or more functional properties of the antibody, such as serum half-life, complement fixation, Fc receptor binding, and/or antigen-dependent cellular cytotoxicity.
  • an antibody of the invention may be chemically modified (e.g. one or more chemical moieties can be attached to the antibody) or be modified to alter its glycosylation, again to alter one or more functional properties of the antibody.
  • the hinge region of CH1 is modified such that the number of cysteine residues in the hinge region is altered, e.g. increased or decreased.
  • This approach is described further in U.S. Patent No. 5,677,425 by Bodmer et al.
  • the number of cysteine residues in the hinge region of CH1 is altered to, for example, facilitate assembly of the light and heavy chains or to increase or decrease the stability of the antibody.
  • the Fc hinge region of an antibody is mutated to decrease the biological half-life of the antibody. More specifically, one or more amino acid mutations are introduced into the CH2-CH3 domain interface region of the Fc-hinge fragment such that the antibody has impaired Staphylococcal protein A (SpA) binding relative to native Fc-hinge domain SpA binding.
  • SpA Staphylococcal protein A
  • the antibody is modified to increase its biological half-life.
  • Various approaches are possible. For example, one or more of the following mutations can be introduced: T252L, T254S, T256F, as described in U.S. Patent No. 6,277,375 to Ward.
  • the antibody can be altered within the CH1 or CL region to contain a salvage receptor binding epitope taken from two loops of a CH2 domain of an Fc region of an IgG, as described in U.S. Patent Nos. 5,869,046 and 6, 121 ,022 by Presta et al.
  • the Fc region is altered by replacing at least one amino acid residue with a different amino acid residue to alter the effector functions of the antibody.
  • one or more amino acids can be replaced with a different amino acid residue such that the antibody has an altered affinity for an effector ligand but retains the antigen-binding ability of the parent antibody.
  • the effector ligand to which affinity is altered can be, for example, an Fc receptor or the C1 component of
  • one or more amino acids selected from amino acid residues can be replaced with a different amino acid residue such that the antibody has altered C1 q binding and/or reduced or abolished complement dependent cytotoxicity (CDC).
  • CDC complement dependent cytotoxicity
  • one or more amino acid residues are altered to thereby alter the ability of the antibody to fix complement. This approach is described further in PCT Publication WO 94/29351 by Bodmer et al.
  • the Fc region is modified to increase the ability of the antibody to mediate antibody dependent cellular cytotoxicity (ADCC) and/or to increase the affinity of the antibody for an Fey receptor by modifying one or more amino acids.
  • ADCC antibody dependent cellular cytotoxicity
  • the Fc domain of lgG1 isotype is used.
  • a mutant variant of lgG1 Fc fragment is used, e.g. a silent lgG1 Fc which reduces or eliminates the ability of the fusion polypeptide to mediate antibody dependent cellular cytotoxicity (ADCC) and/or to bind to an Fey receptor.
  • ADCC antibody dependent cellular cytotoxicity
  • An example of an lgG1 isotype silent mutant wherein Leucine residue is replaced by Alanine residue at amino acid positions 234 and 235 as described by Hezareh et al, J. Virol (2001); 75(24):12161- 8.
  • the Fc domain is a mutant preventing glycosylation at position 297 of Fc domain.
  • the Fc domain contains an amino acid substitution of asparagine residue at position 297.
  • Example of such amino acid substitution is the replacement of N297 by a glycine or an alanine.
  • Silenced effector functions can be obtained by mutation in the Fc region of the antibodies and have been described in the art: LALA and N297A (Strohl, W., 2009, Curr. Opin. Biotechnol. vol. 20(6):685-691); and D265A (Baudino et al., 2008, J. Immunol. 181 :6664-69; Strohl, W., supra); and DAPA (D265A and P329A) (Shields RL, J Biol Chem. 2001 ;276(9):6591-604; U.S. Patent Publication US2015/0320880).
  • Examples of silent Fc lgG1 antibodies comprise the so-called LALA mutant comprising L234A and L235A mutation in the lgG1 Fc amino acid sequence.
  • Another example of a silent lgG1 antibody comprises the D265A mutation.
  • Another example of a silent lgG1 antibody is the so-called DAPA mutant, comprising D265A and P329A mutations to the lgG1 Fc amino acid sequence.
  • Another silent lgG1 antibody comprises the N297A mutation, which results in aglycosylated/non-glycosylated antibodies. Additional Fc mutations for providing silenced effector function are described in PCT publication no.
  • WO2014/145806 (e.g., in Figure 7 of WO2014/145806), herein incorporated by reference in its entirety.
  • WO2014/145806 of a silent lgG1 antibody comprises a E233P, L234V, L235A, and S267K mutation, and a deletion of G236 (G236del).
  • Another example from WO2014/145806 of a silent lgG1 antibody comprises a E233P, L234V, and L235A mutation, and a deletion of G236 (G236del).
  • Another example from WO2014/145806 of a silent lgG1 antibody comprises a E233P, L234V, and L235A mutation, and a deletion of G236 (G236del).
  • WO2014/145806 of a silent lgG1 antibody comprises a S267K mutation.
  • the glycosylation of an antibody is modified.
  • an aglycosylated antibody can be made (i.e., the antibody lacks glycosylation).
  • Glycosylation can be altered to, for example, increase the affinity of the antibody for the antigen.
  • carbohydrate modifications can be accomplished by; for example, altering one or more sites of glycosylation within the antibody sequence.
  • one or more amino acid substitutions can be made that result in elimination of one or more variable region framework glycosylation sites to thereby eliminate glycosylation at that site.
  • Such aglycosylation may increase the affinity of the antibody for antigen.
  • an antibody can be made that has an altered type of glycosylation, such as a hypofucosylated antibody having reduced amounts of fucosyl residues or an antibody having increased bisecting GlcNac structures.
  • altered glycosylation patterns have been demonstrated to increase the ADCC ability of antibodies.
  • carbohydrate modifications can be accomplished by, for example, expressing the antibody in a host cell with altered glycosylation machinery. Cells with altered glycosylation machinery have been described in the art and can be used as host cells in which to express recombinant antibodies of the invention to thereby produce an antibody with altered glycosylation.
  • EP 1 , 176, 195 by Hang et al.
  • the antibodies of the invention are produced by recombinant expression in a cell line which exhibit hypofucosylation pattern, for example, a
  • the antibodies of the invention can be produced in a yeast or a filamentous fungi engineered for mammalian-like glycosylation pattern, and capable of producing antibodies lacking fucose as glycosylation pattern (see for example EP1297172B1).
  • An antibody can be pegylated to, for example, increase the biological (e.g. serum) half-life of the antibody.
  • the antibody, or fragment thereof typically is reacted with polyethylene glycol (PEG), such as a reactive ester or aldehyde derivative of PEG, under conditions in which one or more PEG groups become attached to the antibody or antibody fragment.
  • PEG polyethylene glycol
  • the pegylation can be carried out by an acylation reaction or an alkylation reaction with a reactive PEG molecule (or an analogous reactive water-soluble polymer).
  • polyethylene glycol is intended to encompass any of the forms of PEG that have been used to derivatize other proteins, such as mono (C1-C10) alkoxy- or aryloxy-polyethylene glycol or polyethylene glycol-maleimide.
  • the antibody to be pegylated is an aglycosylated antibody. Methods for pegylating proteins are known in the art and can be applied to the antibodies of the invention. See for example, EP 0 154 316 by
  • Another modification of the antibodies that is contemplated by the invention is a conjugate or a protein fusion of at least the antigen-binding region of the antibody of the invention to serum protein, such as human serum albumin or a fragment thereof to increase half-life of the resulting molecule.
  • serum protein such as human serum albumin or a fragment thereof to increase half-life of the resulting molecule.
  • Another modification of the antibodies that is contemplated by the invention is one or more modifications to increase formation of a heterodimeric bispecific antibody.
  • a variety of approaches available in the art can be used in for enhancing dimerization of the two heavy chain domains of bispecific antibodies, e.g., bbmAbs, as disclosed in, for example, EP 1870459A1 ; U.S. Pat. No. 5,582,996; U.S. Pat. No. 5,731 , 168; U.S. Pat.
  • Multispecific molecules e.g., multispecific antibody or antibody-like molecules, of the present invention may comprise one or more, e.g., a plurality, of mutations to one or more of the constant domains, e.g., to the CH3 domains.
  • the multispecific molecule of the present invention comprises two polypeptides that each comprise a heavy chain constant domain of an antibody, e.g., a CH2 or CH3 domain.
  • the two heavy chain constant domains, e.g., the CH2 or CH3 domains of the multispecific molecule comprise one or more mutations that allow for a heterodimeric association between the two chains.
  • the one or more mutations are disposed on the CH2 domain of the two heavy chains of the multispecific, e.g., bispecific, antibody or antibody-like molecule. In one aspect, the one or more mutations are disposed on the CH3 domains of at least two polypeptides of the multispecific molecule.
  • the one or more mutations to a first polypeptide of the multispecific molecule comprising a heavy chain constant domain creates a "knob" and the one or more mutations to a second polypeptide of the multispecific molecule comprising a heavy chain constant domain creates a "hole,” such that heterodimerization of the polypeptide of the multispecific molecule comprising a heavy chain constant domain causes the "knob" to interface (e.g., interact, e.g., a CH2 domain of a first polypeptide interacting with a CH2 domain of a second polypeptide, or a CH3 domain of a first polypeptide interacting with a CH3 domain of a second polypeptide) with the "hole.”
  • a "knob" refers to at least one amino acid side chain which projects from the interface of a first polypeptide of the multispecific molecule comprising a heavy chain constant domain and is therefore positionable in a compensatory "hole” in the interface with a second poly
  • the knob may exist in the original interface or may be introduced synthetically (e.g. by altering nucleic acid encoding the interface).
  • the preferred import residues for the formation of a knob are generally naturally occurring amino acid residues and are preferably selected from arginine (R), phenylalanine (F), tyrosine (Y) and tryptophan (W). Most preferred are tryptophan and tyrosine.
  • the original residue for the formation of the protuberance has a small side chain volume, such as alanine, asparagine, aspartic acid, glycine, serine, threonine or valine.
  • a "hole” refers to at least one amino acid side chain which is recessed from the interface of a second polypeptide of the multispecific molecule comprising a heavy chain constant domain and therefore accommodates a corresponding knob on the adjacent interfacing surface of a first polypeptide of the multispecific molecule comprising a heavy chain constant domain.
  • the hole may exist in the original interface or may be introduced synthetically (e.g. by altering nucleic acid encoding the interface).
  • the preferred import residues for the formation of a hole are usually naturally occurring amino acid residues and are preferably selected from alanine (A), serine (S), threonine (T) and valine (V). Most preferred are serine, alanine or threonine.
  • the original residue for the formation of the hole has a large side chain volume, such as tyrosine, arginine, phenylalanine or tryptophan.
  • a first CH3 domain is mutated at residue 366, 405 or 407 according to the EU numbering scheme of Edelman et al., PNAS, 1969 May, 63(1):78-85to create either a "knob” or a hole” (as described above), and the second CH3 domain that heterodimerizes with the first CH3 domain is mutated at: residue 407 if residue 366 is mutated in the first CH3 domain, residue 349 if residue 405 is mutated in the first CH3 domain, or residue 366 if residue 407 is mutated in the first CH3 domain, according to the EU numbering scheme of Edelman et al., PNAS, 1969 May, 63(1):78- 85, to create a "hole” or "knob” complementary to the "knob” or "hole” of the first CH3 domain.
  • a first CH3 domain is mutated at residue 366 according to the EU numbering scheme of Edelman et al., PNAS, 1969 May, 63(1):78- 85to create either a "knob” or a hole” (as described above), and the second CH3 domain that heterodimerizes with the first CH3 domain is mutated at residues 366, 368 and/or 407, according to the EU numbering scheme of Edelman et al., PNAS, 1969 May, 63(1):78-85, to create a "hole” or "knob” complementary to the "knob” or "hole” of the first CH3 domain.
  • the mutation to the first CH3 domain introduces a tyrosine (Y) residue at position 366. In an embodiment, the mutation to the first CH3 is T366Y. In one embodiment, the mutation to the first CH3 domain introduces a tryptophan (W) residue at position 366. In an embodiment, the mutation to the first CH3 is T366W.
  • the mutation to the second CH3 domain that heterodimerizes with the first CH3 domain mutated at position 366 comprises a mutation at position 366, a mutation at position 368 and a mutation at position 407, according to the EU numbering scheme of Edelman et al., PNAS, 1969 May, 63(1):78-85
  • the mutation at position 366 introduces a serine (S) residue
  • the mutation at position 368 introduces an alanine (A)
  • the mutation at position 407 introduces a valine (V).
  • the mutations comprise T366S, L368A and Y407V.
  • the first CH3 domain of the multispecific molecule comprises the mutation T366Y, and the second CH3 domain that
  • the heterodimerizes with the first CH3 domain comprises the mutations T366S, L368A and Y407V, or vice versa.
  • the first CH3 domain of the multispecific molecule comprises the mutation T366W, and the second CH3 domain that
  • heterodimerizes with the first CH3 domain comprises the mutations T366S, L368A and Y407V, or vice versa.
  • a KIH variant comprises a first constant chain comprising a L368D and a K370S mutation, paired with a second constant chain comprising a S364K and E357Q mutation.
  • the CH3 domains may be additionally mutated to introduce a pair of cysteine residues. Without being bound by theory, it is believed that the introduction of a pair of cysteine residues capable of forming a disulfide bond provide stability to the heterodimerized multispecific molecule.
  • the first CH3 domain comprises a cysteine at position 354, according to the EU numbering scheme of Edelman et al., PNAS, 1969 May, 63(1):78-85
  • the second CH3 domain that heterodimerizes with the first CH3 domain comprises a cysteine at position 349, according to the EU numbering scheme of Edelman et al.,
  • the first CH3 domain of the multispecific molecule comprises a cysteine at position 354 (e.g., comprises the mutation S354C) and a tyrosine (Y) at position 366 (e.g., comprises the mutation T366Y), and the second CH3 domain that heterodimerizes with the first CH3 domain comprises a cysteine at position 349 (e.g., comprises the mutation Y349C), a serine at position 366 (e.g., comprises the mutation T366S), an alanine at position 368 (e.g., comprises the mutation L368A), and a valine at position 407 (e.g., comprises the mutation Y407V).
  • a cysteine at position 354 e.g., comprises the mutation S354C
  • Y tyrosine
  • T366Y tyrosine
  • the second CH3 domain that heterodimerizes with the first CH3 domain comprises a cysteine at position 349 (e.
  • the first CH3 domain of the multispecific molecule comprises a cysteine at position 354 (e.g., comprises the mutation S354C) and a tryptophan (W) at position 366 (e.g., comprises the mutation T366W), and the second CH3 domain that heterodimerizes with the first CH3 domain comprises a cysteine at position 349 (e.g., comprises the mutation Y349C), a serine at position 366 (e.g., comprises the mutation T366S), an alanine at position 368 (e.g., comprises the mutation L368A), and a valine at position 407 (e.g., comprises the mutation Y407V).
  • a cysteine at position 354 e.g., comprises the mutation S354C
  • W tryptophan
  • T366W tryptophan
  • the second CH3 domain that heterodimerizes with the first CH3 domain comprises a cysteine at position 349 (e.g., comprises
  • heterodimerization of the polypeptide chains (e.g., of the half antibodies) of the multispecific molecule is increased by introducing one or more mutations in a CH3 domain which is derived from the lgG1 antibody class.
  • the mutations comprise a K409R mutation to one CH3 domain paired with F405L mutation in the second CH3 domain, according to the EU numbering scheme of Edelman et al., PNAS, 1969 May, 63(1):78-85. Additional mutations may also, or alternatively, be at positions 366, 368, 370, 399, 405, 407, and 409 according to the EU numbering scheme of Edelman et al., PNAS, 1969 May, 63(1):78-85.
  • heterodimerization of polypeptides comprising such mutations is achieved under reducing conditions, e.g., 10-100 mM 2-MEA (e.g., 25, 50, or 100 mM 2-MEA) for 1-10, e.g., 1.5-5, e.g., 5, hours at 25-37C, e.g., 25C or 37C.
  • reducing conditions e.g., 10-100 mM 2-MEA (e.g., 25, 50, or 100 mM 2-MEA) for 1-10, e.g., 1.5-5, e.g., 5, hours at 25-37C, e.g., 25C or 37C.
  • the amino acid replacements described herein are introduced into the CH3 domains using techniques which are well known in the art. Normally the DNA encoding the heavy chain(s) is genetically engineered using the techniques described in
  • Oligonucleotide-mediated mutagenesis is a preferred method for preparing substitution variants of the DNA encoding the two hybrid heavy chains. This technique is well known in the art as described by Adelman et al., (1983) DNA, 2:183.
  • the IgG heterodimerization strategy is described in, for example,
  • the CH3 domains may be additionally mutated to introduce a pair of cysteine residues. Without being bound by theory, it is believed that the introduction of a pair of cysteine residues capable of forming a disulfide bond provide stability to the heterodimerized multispecific molecule.
  • the first CH3 domain comprises a cysteine at position 354, according to the EU numbering scheme of Edelman et al., PNAS, 1969 May, 63(1):78-85
  • the second CH3 domain that heterodimerizes with the first CH3 domain comprises a cysteine at position 349, according to the EU numbering scheme of Edelman et al., PNAS, 1969 May, 63(1):78-85.
  • heterodimerization of the polypeptide chains (e.g., of the half antibodies) of the multispecific molecule is increased by introducing mutations based on the "polar-bridging" rational, which is to make residues at the binding interface of the two polypeptide chains to interact with residues of similar (or complimentary) physical property in the heterodimer configuration, while with residues of different physical property in the homodimer configuration.
  • these mutations are designed so that, in the heterodimer formation, polar residues interact with polar residues, while hydrophobic residues interact with hydrophobic residues.
  • residues are mutated so that polar residues interact with hydrophobic residues.
  • the above mutations are generated at one or more positions of residues 364, 368, 399, 405, 409, and 411 of CH3 domain, amino acid numbering according to the EU numbering scheme of Edelman et al., PNAS, 1969 May, 63(1):78-85.
  • Ser364Leu, Thr366Val, Leu368Gln, Asp399Lys, Phe405Ser, Lys409Phe and Thr41 1 Lys are introduced into one of the two CH3 domains.
  • Ser364Leu original residue of serine at position 364 is replaced by leucine
  • Thr366Val original residue of threonine at position 366 is replaced by valine
  • Leu368Gln original residue of leucine at position 368 is replaced by glutamine
  • Asp399Lys original residue aspartic acid at position 399 is replaced by lysine
  • Phe405Ser original residue phenylalanine at position 405 is replaced by serine
  • Lys409Phe original residue lysine at position 409 is replaced by
  • Thr41 1 Lys original residue of threonine at position 41 1 is replaced by lysine.
  • the other CH3 can be introduced with one or more mutations selected from a group consisting of: Tyr407Phe, Lys409Gln and Thr41 1Asp (Tyr407Phe: original residue tyrosine at position 407 is replaced by phenyalanine; Lys409Glu: original residue lysine at position 409 is replaced by glutamic acid; Thr41 1Asp: original residue of threonine at position 411 is replaced by aspartic acid).
  • Tyr407Phe original residue tyrosine at position 407 is replaced by phenyalanine
  • Lys409Glu original residue lysine at position 409 is replaced by glutamic acid
  • Thr41 1Asp original residue of threonine at position 411 is replaced by aspartic acid
  • one CH3 domain has one or more mutations selected from a group consisting of: Ser364Leu, Thr366Val, Leu368Gln, Asp399Lys, Phe405Ser, Lys409Phe and Thr411 Lys, while the other CH3 domain has one or more mutations selected from a group consisting of: Tyr407Phe, Lys409Gln and Thr41 1Asp.
  • the original residue of threonine at position 366 of one CH3 domain is replaced by valine, while the original residue of tyrosine at position 407 of the other CH3 domain is replaced by phenylalanine.
  • the original residue of serine at position 364 of one CH3 domain is replaced by leucine, while the original residue of leucine at position 368 of the same CH3 domain is replaced by glutamine.
  • the original residue of phenylalanine at position 405 of one CH3 domain is replaced by serine and the original residue of lysine at position 409 of this CH3 domain is replaced by phenylalanine, while the original residue of lysine at position 409 of the other CH3 domain is replaced by glutamine.
  • the original residue of aspartic acid at position 399 of one CH3 domain is replaced by lysine
  • the original residue of threonine at position 41 1 of the same CH3 domain is replaced by lysine
  • the original residue of threonine at position 411 of the other CH3 domain is replaced by aspartic acid.
  • amino acid replacements described herein are introduced into the CH3 domains using techniques which are well known in the art. Normally the DNA encoding the heavy chain(s) is genetically engineered using the techniques described in Mutagenesis: a Practical Approach. Oligonucleotide-mediated mutagenesis is a preferred method for preparing substitution variants of the DNA encoding the two hybrid heavy chains. This technique is well known in the art as described by Adelman et al., (1983) DNA, 2:183.
  • the polar bridge strategy is described in, for example, WO2006/106905,
  • polar bridge variants are described in, for example, PCT publication no. WO2014/145806 (for example, Figure 6 of WO2014/145806), PCT publication no. WO2014/110601 , and PCT publication no. WO 2016/086186, WO 2016/086189, WO 2016/086196 and WO 2016/182751 the contents of which are incorporated herein in their entireties.
  • An example of a polar bridge variant comprises a constant chain comprising a N208D, Q295E, N384D, Q418E and N421 D mutation.
  • the CH3 domains may be additionally mutated to introduce a pair of cysteine residues. Wthout being bound by theory, it is believed that the introduction of a pair of cysteine residues capable of forming a disulfide bond provide stability to the heterodimerized multispecific molecule.
  • the first CH3 domain comprises a cysteine at position 354, according to the EU numbering scheme of Edelman et al., PNAS, 1969 May, 63(1):78-85
  • the second CH3 domain that heterodimerizes with the first CH3 domain comprises a cysteine at position 349, according to the EU numbering scheme of Edelman et al., PNAS, 1969 May, 63(1):78-85.
  • bispecific antibody e.g., bbmAb
  • the resulting bbmAb, bbmAbl is a bispecific lgG1 , with LALA silencing mutations, simultaneously binding to two distinct targets, I L-1 ⁇ and IL-18.
  • the antibody combines two distinct antigen binding arms (Fab fragments), whereas the Fab directed against I L- 1 ⁇ is based on mAb2 and contains a kappa light chain (Vk6).
  • the Fab directed against IL-18 is based on mAbl and is composed of a lambda light chain (VA1).
  • a "knob” with a bulky amino acid (aa) side chain (S354C and T366W) in the mAbl heavy chain and a "hole” with small aa side chains (Y349C, T366S, L368A, Y407V) were introduced in the mAb2 heavy chain.
  • amino acid sequences of the hypervariable regions of bbmAbl based on the Kabat definition and the Chothia definition, as well as the VL and VH domains and full heavy and light chains are provided in Table 3, below.
  • the IL-18/I L-1 ⁇ bispecific antibody comprises a first immunoglobulin heavy chain variable domain (Vm) comprising hypervariable regions CDR1 , CDR2 and CDR3, said CDR1 having the amino acid sequence SEQ ID NO:76, said CDR2 having the amino acid sequence SEQ ID NO:77, and said CDR3 having the amino acid sequence SEQ ID NO:78.
  • Vm immunoglobulin heavy chain variable domain
  • I L- 18/I L- 1 ⁇ bispecific antibody comprises a first immunoglobulin heavy chain variable domain (Vm) comprising hypervariable regions CDR1 , CDR2 and CDR3, said CDR1 having the amino acid sequence SEQ ID NO:79, said CDR2 having the amino acid sequence SEQ ID NO:80, and said CDR3 having the amino acid sequence SEQ ID NO:81.
  • Vm immunoglobulin heavy chain variable domain
  • IL- 18/I L- 1 ⁇ bispecific antibody comprises a first immunoglobulin heavy chain variable domain (Vm) comprising hypervariable regions CDR1 , CDR2 and CDR3, said CDR1 having the amino acid sequence SEQ ID NO:82, said CDR2 having the amino acid sequence SEQ ID NO:83, and said CDR3 having the amino acid sequence SEQ ID NO:84.
  • Vm immunoglobulin heavy chain variable domain
  • the IL-18/I L-1 ⁇ bispecific antibody comprises a second immunoglobulin heavy chain variable domain (V H 2) comprising hypervariable regions CDR1 , CDR2 and CDR3, said CDR1 having the amino acid sequence SEQ ID NO:44, said CDR2 having the amino acid sequence SEQ ID NO:45, and said CDR3 having the amino acid sequence SEQ ID NO:46.
  • V H 2 immunoglobulin heavy chain variable domain
  • the I L- 18/I L- 1 ⁇ bispecific antibody comprises a second immunoglobulin heavy chain variable domain (VH2) comprising hypervariable regions CDR1 , CDR2 and CDR3, said CDR1 having the amino acid sequence SEQ ID NO:47, said CDR2 having the amino acid sequence SEQ ID NO:48, and said CDR3 having the amino acid sequence SEQ ID NO:49.
  • VH2 immunoglobulin heavy chain variable domain
  • the IL-18/IL-1 ⁇ bispecific antibody comprises a second immunoglobulin heavy chain variable domain (VH2) comprising hypervariable regions CDR1 , CDR2 and CDR3, said CDR1 having the amino acid sequence SEQ ID NO:50, said CDR2 having the amino acid sequence SEQ ID NO:51 , and said CDR3 having the amino acid sequence SEQ ID NO:52.
  • VH2 immunoglobulin heavy chain variable domain
  • the IL-18/I L-1 ⁇ bispecific antibody comprises a first immunoglobulin light chain variable domain (V L i) comprising hypervariable regions CDR1 , CDR2 and CDR3, said CDR1 having the amino acid sequence SEQ ID NO:92, said CDR2 having the amino acid sequence SEQ ID NO:93 and said CDR3 having the amino acid sequence SEQ ID NO:94.
  • V L i immunoglobulin light chain variable domain
  • the IL-18/I L-1 ⁇ bispecific antibody comprises a first immunoglobulin light chain variable domain (Vu) comprising hypervariable regions CDR1 , CDR2 and CDR3, said CDR1 having the amino acid sequence SEQ ID NO:95, said CDR2 having the amino acid sequence SEQ ID NO:96 and said CDR3 having the amino acid sequence SEQ ID NO:97.
  • Vu immunoglobulin light chain variable domain
  • the IL-18/IL-1 ⁇ bispecific antibody comprises a first immunoglobulin light chain variable domain (Vu) comprising hypervariable regions CDR1 , CDR2 and CDR3, said CDR1 having the amino acid sequence SEQ ID NO:98, said CDR2 having the amino acid sequence SEQ ID NO:99 and said CDR3 having the amino acid sequence SEQ ID NO: 100.
  • Vu immunoglobulin light chain variable domain
  • the IL-18/I L-1 ⁇ bispecific antibody comprises a second immunoglobulin light chain variable domain (Vi_2) comprising hypervariable regions CDR1 , CDR2 and CDR3, said CDR1 having the amino acid sequence SEQ ID NO:60, said CDR2 having the amino acid sequence SEQ ID NO:61 and said CDR3 having the amino acid sequence SEQ ID NO:62.
  • Vi_2 immunoglobulin light chain variable domain
  • the IL-18/I L-1 ⁇ bispecific antibody comprises a second immunoglobulin light chain variable domain (V L 2) comprising hypervariable regions CDR1 , CDR2 and CDR3, said CDR1 having the amino acid sequence SEQ ID NO:63, said CDR2 having the amino acid sequence SEQ ID NO:64 and said CDR3 having the amino acid sequence SEQ ID NO:65.
  • V L 2 immunoglobulin light chain variable domain
  • the IL-18/IL-1 ⁇ bispecific antibody comprises a second immunoglobulin light chain variable domain (Vi_2) comprising hypervariable regions CDR1 , CDR2 and CDR3, said CDR1 having the amino acid sequence SEQ ID NO:66, said CDR2 having the amino acid sequence SEQ ID NO:67 and said CDR3 having the amino acid sequence SEQ ID NO:68.
  • Vi_2 immunoglobulin light chain variable domain
  • the I L-18/IL-1 ⁇ bispecific antibody comprises a first immunoglobulin Vm domain and a first immunoglobulin Vu domain, wherein: a) the first immunoglobulin Vm domain comprises (e.g. in sequence): i) hypervariable regions CDR1 , CDR2 and CDR3, said CDR1 having the amino acid sequence SEQ ID NO:76, said CDR2 having the amino acid sequence SEQ ID NO:77, and said CDR3 having the amino acid sequence SEQ ID NO:78; or ii) hypervariable regions CDR1 , CDR2 and CDR3, said CDR1 having the amino acid sequence SEQ ID NO:79, said CDR2 having the amino acid sequence SEQ ID NO:80, and said CDR3 having the amino acid sequence SEQ ID N0:81 ; or iii) hypervariable regions CDR1 , CDR2 and CDR3, said CDR1 having the amino acid sequence SEQ ID NO:82, said CDR2 having the amino acid sequence SEQ ID NO:
  • the I L-18/IL-1 ⁇ bispecific antibody comprises a second immunoglobulin VH2 domain and a second immunoglobulin Vi_2 domain, wherein: a) the second immunoglobulin VH2 domain comprises (e.g. in sequence): i) hypervariable regions CDR1 , CDR2 and CDR3, said CDR1 having the amino acid sequence SEQ ID NO:44, said CDR2 having the amino acid sequence SEQ ID NO:45, and said CDR3 having the amino acid sequence SEQ ID NO:46; or ii) hypervariable regions CDR1 , CDR2 and CDR3, said CDR1 having the amino acid sequence SEQ ID NO:47, said CDR2 having the amino acid sequence SEQ ID NO:48, and said CDR3 having the amino acid sequence SEQ ID NO:49; or iii) hypervariable regions CDR1 , CDR2 and CDR3, said CDR1 having the amino acid sequence SEQ ID NO:50, said CDR2 having the amino acid sequence SEQ ID NO:50
  • the IL-18/1 L-1 ⁇ bispecific antibody comprises: a) a first immunoglobulin heavy chain variable domain (V H i) comprising the amino acid sequence set forth as SEQ ID NO:85; b) a first immunoglobulin light chain variable domain (V L i) comprising the amino acid sequence set forth as SEQ ID NO: 101 ; c) a first immunoglobulin heavy chain variable domain (V H i) comprising the amino acid sequence set forth as SEQ ID NO:85; b) a first immunoglobulin light chain variable domain (V L i) comprising the amino acid sequence set forth as SEQ ID NO: 101 ; c) a first immunoglobulin heavy chain variable domain (V H i) comprising the amino acid sequence set forth as SEQ ID NO:85; b) a first immunoglobulin light chain variable domain (V L i) comprising the amino acid sequence set forth as SEQ ID NO: 101 ; c) a first immunoglobulin heavy chain variable domain (V H i) compris
  • immunoglobulin V H i domain comprising the amino acid sequence set forth as SEQ ID NO:85 and a first immunoglobulin V L i domain comprising the amino acid sequence set forth as SEQ ID NO: 101 ; d) a first immunoglobulin Vm domain comprising the hypervariable regions set forth as SEQ ID NO:76, SEQ ID NO:77, and SEQ ID NO:78; e) a first immunoglobulin Vu domain comprising the hypervariable regions set forth as SEQ ID NO:92, SEQ ID NO:93 and SEQ ID NO:94; f) a first immunoglobulin Vm domain comprising the hypervariable regions set forth as SEQ ID NO:79, SEQ ID NO:80 and SEQ ID NO:81 ; g) a first immunoglobulin Vu domain comprising the hypervariable regions set forth as SEQ ID NO:95, SEQ ID NO:96 and SEQ ID NO:97; h) a first immunoglobulin Vm domain comprising the hypervariable regions set forth
  • the IL-18/I L-1 ⁇ bispecific antibody comprises: a) a second immunoglobulin heavy chain variable domain (V H 2) comprising the amino acid sequence set forth as SEQ ID NO:53; b) a second immunoglobulin light chain variable domain (Vi_2) comprising the amino acid sequence set forth as SEQ ID NO:69; c) a second immunoglobulin heavy chain variable domain (V H 2) comprising the amino acid sequence set forth as SEQ ID NO:53; b) a second immunoglobulin light chain variable domain (Vi_2) comprising the amino acid sequence set forth as SEQ ID NO:69; c) a second immunoglobulin heavy chain variable domain (V H 2) comprising the amino acid sequence set forth as SEQ ID NO:53; b) a second immunoglobulin light chain variable domain (Vi_2) comprising the amino acid sequence set forth as SEQ ID NO:69; c) a second immunoglobulin heavy chain variable domain (V H 2) comprising the amino acid sequence set forth as SEQ ID NO:
  • immunoglobulin VH2 domain comprising the amino acid sequence set forth as SEQ ID NO:53 and a second immunoglobulin Vi_2 domain comprising the amino acid sequence set forth as SEQ ID NO:69; d) a second immunoglobulin VH2 domain comprising the hypervariable regions set forth as SEQ ID NO:44, SEQ ID NO:45, and SEQ ID NO:46; e) a second immunoglobulin Vi_2 domain comprising the hypervariable regions set forth as SEQ ID NO:60, SEQ ID NO:61 and SEQ ID NO:62; f) a second immunoglobulin V H2 domain comprising the hypervariable regions set forth as SEQ ID NO:47, SEQ ID NO:48 and SEQ ID NO:49; g) a second immunoglobulin Vi_2 domain comprising the
  • the I L- 18/I L- 1 ⁇ bispecific antibody comprises a the three CDRs of SEQ ID NO:53.
  • the IL-18/IL-1 ⁇ bispecific antibody comprises the three CDRs of SEQ ID NO:69.
  • the IL-18/IL-1 ⁇ bispecific antibody comprises the three CDRs of SEQ ID NO:53 and the three CDRs of SEQ ID NO:69.
  • the IL-18/IL-1 ⁇ bispecific antibody comprises the three CDRs of SEQ ID NO:85.
  • the IL-18/IL-1 ⁇ bispecific antibody comprises the three CDRs of SEQ ID NO: 101.
  • the ⁇ _-18/ ⁇ _-1 ⁇ bispecific antibody comprises the three CDRs of SEQ ID NO:85 and the three CDRs of SEQ ID NO: 101.
  • the I L- 18/I L- 1 ⁇ bispecific antibody comprises a the three CDRs of SEQ ID NO:85. In other embodiments, the IL-18/IL-1 ⁇ bispecific antibody comprises the three CDRs of SEQ ID NO: 101. In other embodiments, the I L- 18/I L- 1 ⁇ bispecific antibody comprises the three CDRs of SEQ ID NO:85 and the three CDRs of SEQ ID NO: 101. In some embodiments, the I L-18/IL-1 ⁇ bispecific antibody comprises the three CDRs of SEQ ID NO:53. In other embodiments, the ⁇ _-18/ ⁇ _-1 ⁇ bispecific antibody comprises the three CDRs of SEQ ID NO:69.
  • the IL- 18/I L-1 ⁇ bispecific antibody comprises the three CDRs of SEQ ID NO:53 and the three CDRs of SEQ ID NO:69.
  • the L- 18/I L- 1 ⁇ bispecific antibody comprises the three CDRs of SEQ ID NO:85, the three CDRs of SEQ ID NO: 101 , the three CDRs of SEQ ID NO:53 and the three CDRs of SEQ ID NO:69.
  • the first part of the IL-18/IL-1 ⁇ bispecific antibody is selected from a human I L-18 antibody that comprises at least: a) an immunoglobulin heavy chain or fragment thereof which comprises a variable domain comprising, in sequence, the hypervariable regions CDR1 , CDR2 and CDR3 and the constant part or fragment thereof of a human heavy chain; said CDR1 having the amino acid sequence SEQ ID NO:76, said CDR2 having the amino acid sequence SEQ ID NO:77, and said CDR3 having the amino acid sequence SEQ ID NO:78; and b) an immunoglobulin light chain or fragment thereof which comprises a variable domain comprising, in sequence, the hypervariable regions CDR1 , CDR2, and CDR3 and the constant part or fragment thereof of a human light chain, said CDR1 having the amino acid sequence SEQ ID NO:92, said CDR2 having the amino acid sequence SEQ ID NO:93, and said CDR3 having the amino acid sequence SEQ ID NO:94.
  • the second part of the I L-18/IL-1 ⁇ bispecific antibody is selected from a human I L- 1 ⁇ antibody that comprises at least: a) an immunoglobulin heavy chain or fragment thereof which comprises a variable domain comprising, in sequence, the hypervariable regions CDR1 , CDR2 and CDR3 and the constant part or fragment thereof of a human heavy chain; said CDR1 having the amino acid sequence SEQ ID NO:44, said CDR2 having the amino acid sequence SEQ ID NO:45, and said CDR3 having the amino acid sequence SEQ ID NO:46; and b) an immunoglobulin light chain or fragment thereof which comprises a variable domain comprising, in sequence, the hypervariable regions CDR1 , CDR2, and CDR3 and the constant part or fragment thereof of a human light chain, said CDR1 having the amino acid sequence SEQ ID NO:60, said CDR2 having the amino acid sequence SEQ ID NO:61 , and said CDR3 having the amino acid sequence SEQ ID NO:62.
  • the first part of the IL-18/IL-1 ⁇ bispecific antibody is selected from a human IL-18 antibody that comprises at least: a) an immunoglobulin heavy chain or fragment thereof which comprises a variable domain comprising, in sequence, the hypervariable regions CDR1 , CDR2 and CDR3 and the constant part or fragment thereof of a human heavy chain; said CDR1 having the amino acid sequence SEQ ID NO:76, said CDR2 having the amino acid sequence SEQ ID NO:77 and said CDR3 having the amino acid sequence SEQ ID NO:78; and b) an immunoglobulin light chain or fragment thereof which comprises a variable domain comprising, in sequence, the hypervariable regions CDR1 , CDR2, and CDR3 and the constant part or fragment thereof of a human light chain, said CDR1 having the amino acid sequence SEQ ID NO:92, said CDR2 having the amino acid sequence SEQ ID NO:93, and said CDR3 having the amino acid sequence SEQ ID NO:94.
  • the second part of the I L- 18/I L- 1 ⁇ bispecific antibody is selected from a human I L- 1 ⁇ antibody that comprises at least: a) an immunoglobulin heavy chain or fragment thereof which comprises a variable domain comprising, in sequence, the hypervariable regions CDR1 , CDR2 and CDR3 and the constant part or fragment thereof of a human heavy chain; said CDR1 having the amino acid sequence SEQ ID NO:44, said CDR2 having the amino acid sequence SEQ ID NO:45 and said CDR3 having the amino acid sequence SEQ ID NO:46; and b) an immunoglobulin light chain or fragment thereof which comprises a variable domain comprising, in sequence, the hypervariable regions CDR1 , CDR2, and CDR3 and the constant part or fragment thereof of a human light chain, said CDR1 having the amino acid sequence SEQ ID NO:60, said CDR2 having the amino acid sequence SEQ ID NO:61 , and said CDR3 having the amino acid sequence SEQ ID NO:62.
  • the first Vm or VLI domain of an I L-18/I L-1 ⁇ bispecific antibody used in the disclosed methods may have a first Vm and/or first V L i domains that are substantially identical to the V H or V L domains set forth in SEQ ID NO:85 and 101.
  • An IL-18/IL-1 ⁇ bispecific antibody disclosed herein may comprise a first heavy chain that is substantially identical to that set forth as SEQ ID NO:87 and/or a first light chain that is substantially identical to that set forth as SEQ ID NO: 103.
  • An ⁇ _-18/ ⁇ _-1 ⁇ bispecific antibody disclosed herein may comprise a first heavy chain that comprises SEQ ID NO:87 and a first light chain that comprises SEQ ID NO: 103.
  • An IL-18/I L-1 ⁇ bispecific antibody disclosed herein may comprise: a) a first heavy chain, comprising a variable domain having an amino acid sequence substantially identical to that shown in SEQ ID NO:85 and the constant part of a human heavy chain having a hetero-dimerization modification; and b) a first light chain, comprising a variable domain having an amino acid sequence substantially identical to that shown in SEQ ID NO: 101 and the constant part of a human light chain.
  • the constant part of the human heavy chain may be IgGl In one
  • the lgG1 is a human lgG1 without effector mutations.
  • the human heavy chain lgG1 comprising a silencing mutation N297A, D265A or a combination of L234A and L235A.
  • the human heavy chain lgG1 comprises the silencing mutation which is a combination of L234A and L235A, according to SEQ ID NO:87.
  • the second V H 2 or V L 2 domain of an I L-18/I L-1 ⁇ bispecific antibody used in the disclosed methods may have a second V H 2 and/or first V L 2 domains that are substantially identical to the V H or V L domains set forth in SEQ ID NO:53 and 69.
  • An I L- 18/I L- 1 ⁇ bispecific antibody disclosed herein may comprise a second heavy chain that is substantially identical to that set forth as SEQ ID NO:55 and/or a second light chain that is substantially identical to that set forth as SEQ ID NO:71.
  • An IL-18/IL-1 ⁇ bispecific antibody disclosed herein may comprise a second heavy chain that comprises SEQ ID NO:53 and a second light chain that comprises SEQ ID NO:69.
  • An IL-ie/IL- ⁇ ⁇ bispecific antibody disclosed herein may comprise: a) a second heavy chain, comprising a variable domain having an amino acid sequence substantially identical to that shown in SEQ ID NO:53 and the constant part of a human heavy chain having a hetero-dimerization modification, which is complementary to the hetero-dimerization of the first heavy chain; and b) a second light chain, comprising a variable domain having an amino acid sequence substantially identical to that shown in SEQ ID NO:69 and the constant part of a human light chain.
  • the constant part of the human heavy chain may be IgGl
  • the lgG1 is a human lgG1 without effector mutations.
  • the human heavy chain lgG1 comprising a silencing mutation N297A, D265A or a combination of L234A and L235A.
  • the human heavy chain lgG1 comprises the silencing mutation which is a combination of L234A and L235A, according to SEQ ID NO:55.
  • IL-18 antagonists for use as the first part of a bispecific antibody in the disclosed methods, kits and regimens are those set forth in US Patent No: 9,376,489, which is incorporated by reference herein in its entirety.
  • I L-1 ⁇ antagonists e.g. antibodies
  • preferred I L-1 ⁇ antagonists for use as the second part of a bispecific in the disclosed methods, kits and regimens are those set forth in US Patent Nos: 7,446, 175 or 7,993,878 or 8,273,350, which are incorporated by reference herein in their entirety.
  • Vector A was designed for the antibody portion mAbl (anti-IL18 lgG1).
  • the constant region of the heavy chain 1 was modified by two point mutations, T to W as seen in position 366 of SEQ ID NO: 87, and S to C as seen in position 354 of SEQ ID NO: 87, for generating a knob structure and enabling Cys-bridging.
  • the constant region of the heavy chain 1 was modified by with point mutations, L to A, as seen in position 234 of SEQ ID NO: 87 and L to A as seen in position 235 of SEQ ID NO: 87 (so called LALA), for partial silencing of FC effector functions.
  • the antibody has a variable light region, which is of the lambda 1 type, VA1.
  • Vector B was designed for the antibody portion mAb2 (anti I L-1 ⁇ lgG1).
  • the constant region of heavy chain 2 was modified by four point mutations T to S as seen in position 366 of SEQ ID NO: 55, L to A as seen in position 368 of SEQ ID NO: 55, Y to V as seen in position 407 of SEQ ID NO: 55, and Y to C as seen in position 349 of SEQ ID NO: 55, for generating a hole structure and enabling an additional Cys-bridging.
  • the hole structure interacts with the knob structure, to facilitate generation of a bispecific antibody.
  • the constant region of heavy chain 2 was modified with the two LALA mutations, L to A as seen in position 234 of SEQ ID NO: 55, L to A as seen in position 235 of SEQ ID NO: 55, for silencing of FC effector functions.
  • the antibody has a variable light region, which is of the kappa 6 type, VK6.
  • Vector A and B carry a combination of DHFR and neomycin selection markers as well as FOLR and hygromycin selection markers, respectively.
  • Folic acid is a vitamin essential for purine and methionine synthesis and needs to be taken up by mammalian cells from the culture medium.
  • the "folic acid receptor" (FoIR) present on the expression plasmid A is a mutant FoIR with altered affinity for folates, facilitating the transport of folic acid from the medium into mammalian cells. Given that high affinity folic acid receptors are only weakly expressed in cultured CHO cells, cells expressing recombinant FoIR have a clear growth advantage under low folate conditions (50 nM).
  • the FoIR selection marker was encoded at vector B
  • DHFR dihydrofolate reductase
  • MTX is a chemical analogue of folic acid. It is competing for free binding sites on DHFR and thereby blocking the enzyme. Cells overexpressing exogenous DHFR can deal with elevated MTX concentrations, giving the cells a clear selective advantage growing in medium supplemented with MTX.
  • MTX is well known to a person skilled in the art and disclosed e.g. in the patent document WO2010/097239A1 , incorporated here by reference in its entirety.
  • Expression vectors are well known to a person skilled in the art, and disclosed e.g. in the patent document WO2009/080720A1 , incorporated here by reference in its entirety.
  • a parental CHO cell line was used as host cell line for the production of the bbmAbl expressing cell line.
  • the host cell line was derived from the CHO-K1 cell line, well known to a person skilled in the art, in a way described e.g. in the patent applications WO2015092737 and WO2015092735, both incorporated by reference in their entirety.
  • a single vial from the CHO line was used to prepare the bbmAbl recombinant cell line.
  • the CHO line was prepared in chemically defined medium.
  • the cells were grown in chemically defined cultivation medium.
  • Swal linearized plasmid DNA expression vector A & B encoding for the bbmAb, was added per transfection.
  • the transfection reaction was performed in chemically defined cultivation medium.
  • Transfections were performed by electroporation using an AMAXA Gene Pulser, according to the manufactures instructions.
  • the parental CHO cells used for transfection were in exponential growth phase with cell viabilities higher than 95%. In total, three transfections were performed with 5x10 6 cells per transfection.
  • a selection procedure was carried out using the selection marker encoded by the individual expression vector A and B, as described above.
  • Both proteins are participating in the same molecular pathway; the FoIR is transporting folic acid as well as the folate analogue MTX into the cell, the DHFR is converting it into vital precursors for purine and methionine synthesis. Combining them as selective principle, a particular strong selective regime can be taken to enrich for recombinant cells expressing both recombinant protein.
  • Protein A HPLC methodology was used to determine complete all kind of product and related impurities carrying a Fc part, whereas a reversed phase chromatography (RPC) was use to obtain a fingerprint with respect to the distribution of the individual fractions - individual peaks have been assigned by MS methodology.
  • RPC reversed phase chromatography
  • CHO cell pools producing bbmAbl have been used for a FACS cloning procedure to obtain individualized clonal cell lines as starting material for all further evaluations.
  • Cell selection using FACS analysis is described e.g. in the patent application US201 10281751 , incorporated here by reference in its entirety.
  • Fluorescent-activated cell sorting To enable the FACS sorting the cells were incubated with a FITC-labeled anti lgG1 Fab for 30 min and washed twice in PBS before being used in FACS assisted single cell sorting, a process well known to a person skilled in the art.
  • FACS cell sorting was performed with a FACS Aria (Becton Dickinson), equipped with an
  • ACDU Automatic Cell Deposition Unit
  • the Purity Mask was set to the maximum, so only drops free of particles or further cells were sorted.
  • the Phase Mask was set to half the maximum, so only particles centered within the sorted drop were deflected. Drop trajectory and count accuracy were optimized at the expense of yield to increase the probability that each droplet contained not more than 1 single cell.
  • the clones were handled by a robotic system for the first weeks and handled manually later on, step wise expanded from 96-well, 24-well towards shaker, and finally bioreactor cultivations, well known to a person skilled in the art, to assess performance (productivity and quality of bbmAb expression).
  • MTX Methotrexate
  • Isolated bbmAb from pools and clones were carefully evaluated by different analytical methodologies to judge product characteristics and quality parameters to ensure the selection of the best suited production clone.
  • Upstream processing bbmAb material was produced either in shake flasks or wave fed batch cultures. Frozen stock of pools or clones such as PSLs were thawed and expanded over the required period of time in chemically defined medium to obtain the required number of cells to seed the production culture, typical with a seed cell density of 4.0 x 10 5 cells/ml. The individual culture was cultivated over a period of 13-14 day. During the cultivation in- process controls were performed to monitor the concentration of the bbmAb and the quality profile in the supernatant. At the end of the cultivation process cells were separated from the culture supernatant either by centrifugation (e.g. shaker) or deep filtration followed by sterile filtration before further DSP processing. (b) Downstream processing
  • bbmAbl and bbmAbl variants carrying Fc-part were captured from cell free supernatant by a first affinity liquid chromatography (ALC) step on MabSelectTM SuReTM.
  • AAC affinity liquid chromatography
  • bbmAbl variants containing kappa light chains only (mAb2 hole, Figure 4C and 4D) and HCP were removed by a first polish on LambdaFabSelectTM and bbmAbl variants containing lambda light chains only (mAbl knob, Figure 4A and 4B) and HCP were removed by a second polish step on KappaSelectTM.
  • Chromatography was performed at room temperature using 4 minutes residence time (RT) throughout the method. All columns were equilibrated with 4 column volumes (CV) 20 mM Na 2 HP0 4 /NaH 2 P04, pH 7.0 before loading. To deplete unspecific bound impurities from product, such as host cell proteins (HCP), media components and DNA the chromatography column was washed with 4 CV 250 mM Arginine-HCI, 1 M NaCI, 88 mM NaOH, pH 9.0 and 3 CV equilibration buffer after loading cell-free bbmAbl supernatant from shakeflask onto the ALC column.
  • CV column volumes
  • HCP host cell proteins
  • bbmAbl and potential bbmAbl variants were eluted from the chromatographic column by using 50 mM acetic acid, pH 3.0 respectively 50 mM acetic acid/HCI, pH 2.0 for elution from KappaSelectTM and LambdaFabSelectTM.
  • Product peak collection started and ended at 0.5 AU/cm or 0.25 AU/cm (280 nm).
  • the pH of the bbmAbl eluates were adjusted to ⁇ pH 5.0 with 0.1 or 1 M Tris prior to storage at 2-8°C respectively for analytical assessment.
  • LambdaFabSelectTM In order to inactivate potentially present enveloped viruses a low pH treatment of the ALC eluate was performed followed by two chromatographic polish steps on Capto adhere and FractogelTM EMD SC to remove product related impurities, DNA and HCP. Potentially present viruses were subsequently removed by nanofiltration prior to a final concentration and buffer exchange step using tangential-flow filtration. a) Affinity Liquid Chromatography (ALC) on LambdaFabSelectTM
  • ALC was performed at 18-28°C using 3.6-4.4 minutes residence time (RT) throughout the method.
  • the ALC column was equilibrated with 4-6 CV 20 mM Na2HP04/NaH2P04, pH 7.0.
  • clarified cell-free bbmAbl supernatant from waves or bioreactors was loaded onto the LambdaFabSelectTM column with a loading density of 7- 23 g/L.
  • a column wash with 4-6 CV 250 mM Arginine-HCI, 1 M NaCI, 88 mM NaOH, pH 9.0 and a second wash with 3-5 CV equilibration buffer was performed prior to product elution with 4-6 CV 50 mM acetic acid.
  • the product peak was collected from 0.5-2.0 AU/cm (280 nm) ascending and 0.5-2.0 AU/cm (280 nm) descending.
  • the product peak was collected from 0.5-2.0 AU/cm (280 nm) ascend
  • LambdaFabSelectTM column was cleaned using 3-5 CV 120 mM phosphoric acid, 167 mM acetic acid, pH 1.5 prior to re-equilibration with 3-5 CV 20 mM Na 2 HP0 4 /NaH 2 P04, pH 7.0 and storage in 4-6 CV 20 % ethanol.
  • MAC was performed in flow-through mode at 18-28°C using 4-6 minutes residence time throughout the method.
  • the MAC column was equilibrated with 7-9 CV 20 mM Tris/Tris-HCI, pH 7.5.
  • the low pH treated ALC eluate was loaded onto the CaptoTM adhere column using a loading density of 175-350 g/L.
  • Product peak collection started thereby at 0.5-2.0 AU/cm (280nm) ascending.
  • the MAC column was washed with 5-7 CV equilibration buffer and product peak collection ended at 0.5- 2.0 AU/cm (280nm) descending.
  • CaptoTM adhere column was subsequently stripped with 6-8 CV 100 mM acetic acid, followed by a cleaning-in place step with 3-5 CV 0.5 M NaOH and storage in 3-5 CV 0.1 M NaOH. d) Cation Exchange Chromatography on FractogelTM EMD SO3.
  • a tangential-flow filtration step on MilliporeTM PelliconTM 3 RC 30 kDa membranes was performed at 18-28°C.
  • the nanofiltered bbmAbl protein solution was concentrated with a maximum loading density of 1000g/m 2 to 60-80 g/L using a feed flow pressure of 0.5 to 1.2 bar and a
  • transmembrane pressure TMP
  • bbmAbl transmembrane pressure
  • bbmAbl was diafiltered against 7- 9 diafiltration volumes 20 mM histidine/histidine-HCI, pH 6.0 at a feed flow pressure of 0.8 to 1.8 bar and a TMP of 0.4-0.9 bar.
  • a second concentration step to 134 ⁇ 10 g/L at a feed flow pressure of 1.4-3.0 bar and a TMP of 0.7 to 1.5 bar was performed
  • the ultrafiltered bbmAbl protein solution was finally formulated to a concentration of 100 ⁇ 10 g/L and 0.04 % (w/v) Polysorbat 20.
  • the final drug substance (DS) is filtered through a 0.2 ⁇ filter is stored frozen at ⁇ -60°C.
  • MassPREP Micro Desalting column 2.1x 5 mm (Waters) was used at 80°C column temperature. A linear gradient was applied at 0.3 ml/min with mobile phase A: 0.1 % formic acid in water, mobile phase B: 0.1 % FA in acetonitrile: 0-2 min 5% B , 2-12 min 5- 90% B, followed by several wash steps at 0.5 ml/min.
  • Intact de-glycosylated bbmAb Purified bbmAbl antibody was diluted in 20 mM Tris-HCI pH 7.5 to 1 mg/ml and was de-glycosylated for 4 h at 37°C using 2 ⁇ PNGaseF enzyme (New England Biolabs). The digestion was stopped by adding trifluoroacetic acid (TFA) to 2%.
  • TFA trifluoroacetic acid
  • bbmAbl was diluted to 5 mg/ml in 20 ⁇ 0.1 M Tris-HCI pH 7.5. 2 ⁇ of PNGase F was added and incubated for 4 h at 37 °C, then 80 ⁇ of denaturation buffer (50 mM Tris-HCI pH 8.0, 6 M guanidine hydrochloride) and 1 ⁇ of 1 M DTT was added to the mixture. After incubation for 1 hour at 37°C, the sample was acidified with 1 ⁇ TFA.
  • denaturation buffer 50 mM Tris-HCI pH 8.0, 6 M guanidine hydrochloride
  • bbmAbl Papain digestion of bbmAb into Fab and Fc.
  • bbmAbl was mixed with digestion buffer (20 mM succinic acid, 35.1 mM NaOH, pH 6.0, 1 mM Cys-HCI, 1 mM EDTA) to 5 mg/ml, then papain protease (Roche, Germany) was added to final concentration of 5 ⁇ (protease/ protein ratio 1 : 1000) and incubated for 2 hours at
  • TFA was added to quench the digestion.
  • Peptide digests were analyzed by RP-LC-MS on H-Class UPLC (Waters) coupled to a Synapt G2 Q-TOF mass spectrometer (Waters) using an CSH130 C18 2.1 mm x 150, 1.7 ⁇ (Waters, Milford).
  • Mobile phase A 0.1 % TFA in water
  • mobile phase B 0.09 % TFA in acetonitrile.
  • Peptides were eluted from the column with a following gradient: 0-5 min 0%B, 5-10 min 0-2%B, 10-40 min 2-20%B, 40-120 min 20-40%B, 120- 135 min 40-70% at 40°C column temperature.
  • UV chromatograms were recorded at 214 nm and MS data acquisition was performed in positive ES(+) resolution mode as an MS E experiment using low energy (4 eV) and high energy fragmentation (30-55 V).
  • Lock mass was Leucin Enkephalin (Waters).
  • Data processing and evaluation was performed by MassLynx 4.2 or UNIFI 1.6 software (Waters). MaxEntl algorithm was used for deconvolution of protein mass spectra. Theoretical mass calculations were performed with GPMAW 9.2 software (Lighthouse data).
  • bbmAbl sample were subjected to TSK gel G3000SWXL (Tosoh #808541 , 5 ⁇ ,
  • sample buffer 0.1 sodium phosphate/ 1.0 % SDS, pH 6.6
  • iodoacetamide solution 0.1 sodium phosphate/ 1.0 % SDS, pH 6.6
  • the protein was mixed with 0.1 M Tris/ 1 % SDS sample buffer, pH 8.0 and reduced with 5 % (v/v) mercaptoethanol. Both samples underwent a heat denaturation step at 70°C for 10 minutes.
  • Electropherograms were processed and integrated using ChromeleonTM 6.8 software.
  • Designation I is lambda chain, k is kappa chain.
  • mAb3 is an antibody of type VH3, Vk1.
  • mAb4 is an antibody of type VH3, Vk1.
  • mAb5 is a graft version of mAbl (VH1 , VI1).
  • mAb6 is a graft version of mAb2 (VH1_46, Vk3).
  • mAb7 is an antibody of type VH3, Vk1.
  • mAb8 is an antibody of type VH1_2, Vk2.
  • mAb9 is an antibody of type VH5, Vk6.
  • mAb10 is an antibody of type VH1_46, Vk6.
  • mAb11 is an antibody of type VH3, Vk3.
  • mAb12 is an antibody of type VH3, Vk2.
  • bbmAbl was characterized in more detail to evaluate the all formed product variants and impurities after the different purification steps by LC-MS using several sample preparation methods, and with other separation techniques.
  • the mass of the intact 2-step (lambda/CEC) purified product was determined after de-glycosylation with PNGaseF enzyme and subsequent injection into the RP-LC-MS setup.
  • the de- convoluted mass spectrum of intact bbmAbl confirmed the correct formation of the knob- in-hole heterodimer after co-expression and lambda-select purification. No major impurities like homodimers or partial antibodies could be detected after lambda-select purification. Identity of the four different antibody chains could be confirmed after reduction and de-glycosylation of the sample.
  • the purified sample was digested by papain protease to analyze the individual Fab and Fc fragments.
  • the measured masses of the fragments confirmed again the correct formation of the different Fab arms (Knob-lambda, Hole-kappa), as well as the correct formation of the knob-in- hole Fc fragment.
  • a mispaired Fab fragment (Fab4, Knob-kappa) could be observed in levels ⁇ 1 %.
  • An alternative approach the generate Fab fragments with a limited LysC digestion was tested which allows faster sample prep during pool and clone selection.
  • LysC peptide mapping with LC-MS could confirm identity of the molecule with an overall sequence coverage of the peptides of 99%.
  • Figure 3A is a chromatogram showing the expression profile after cultivation
  • Figure 3B is a chromatogram after capturing by LambdaFabSelectTM
  • Figure 3C is a chromatogram after capturing with MabSelectTM SuReTM
  • Figure 3D is a chromatogram after capturing with LambdaFabSelectTM, polish by FractogelTM EMD SO 3 and ultrafiltration.
  • the final 2-step purified (lambda/CEC) bispecific bbmAbl was further analyzed by methods listed in Table 6. Overall the material shows high purity with low level of aggregates or degradation products as detected by several separation methods such as size exclusion chromatography (SEC), CE-SDS and capillary zone electrophoresis (CZE). Table 6. Purity analytics of purified bbmAbl
  • Table 4 shows a summary of analytical results obtained.
  • Binding activity of bbmAbl was tested in a variety of different cell assays.
  • MSD read buffer T with surfactant MSD #R92TC-1)
  • PBS Phosphate-buffered saline
  • TBS Tris-buffered saline
  • Tween-20 Tween-20
  • MTP Polypropylene microtiter plate
  • Recombinant human IL-18 (BTP 25829) purchased from MBL Int. Corp. (#B001-5) Recombinant marmoset I L-1 ⁇ (Novartis)
  • Recombinant human IL-12 (#573008) was purchased from Biolegend KG-1 cell line (ATCC #CCL-246)
  • HEK-BlueTM IL-18/IL-1 ⁇ cells (#hkb-il18) were purchased from InvivoGen
  • PBMC peripheral blood mononuclear cells
  • IL-6 ELISA Human (BioLegend, #430503); Marmoset (U-CyTech biosciences, CT974-5) IFNy ELISA: Human (BD555142) and marmoset (U-CyTech biosciences #CT340A) QUANTI-BlueTM assays (#rep-qb1) for the detection of SEAP was purchased from InvivoGen
  • Cell medium RPMI 1640 (Invitrogen #31870) supplemented with 10% Foetal Bovine Serum (Invitrogen #10108-157), 1 % L-Glutamine (Invitrogen #25030-03), 1 % penicillin/ streptomycin (Invitrogen #15140-148), 10 ⁇ 2-Mercaptoethanol (Gibco #31350-010), 5mM Hepes (Gibco #15630-080)
  • Ficoll-PacqueTM Plus (GE Healthcare Life Sciences #17-1440-02) PBS 1X, without Calcium & Magnesium (Gibco #14190094)
  • electrochemiluminescence signal without antigen, B ma x) The plate was sealed and incubated overnight (o/n, at least 16 h) at room temperature (RT) on a shaker.
  • IL-18 readout A streptavidin coated 384-well MSD array MTP was coated with 30 ⁇ /well biotinylated hulL-18 (0.1 ⁇ , PBS) and incubated for 1 h at RT on a shaker.
  • IL-1 ⁇ readout A standard 384-well MSD array MTP was coated with 30 ⁇ /well of hulL-1 (3 ⁇ / ⁇ , PBS) diluted in PBS as capture agent and incubated overnight at 4°C.
  • the plate was blocked with 50 ⁇ /well blocking buffer (PBS containing 5 % BSA) for 1 hour (h), at room temperature (RT). After washing (TBST, TBS containing 0.05 % Tween 20), a volume of 30 ⁇ /well of the equilibrated antigen- antibody mix was transferred from the polypropylene MTP to the coated MSD plate and incubated for 20 min at RT. After an additional wash step, 30 ⁇ sulfo tag-labeled anti-lgG detection antibody (0.5 ⁇ g/ml) diluted in sample buffer were added to each well and incubated for 30 min at RT on a shaker. The MSD plate was washed and 35 ⁇ / ⁇ MSD read buffer were added and incubated for 5 min at RT. Electrochemiluminescence (ECL) signals were generated and measured by the MSD Sector Imager 6000.
  • the SET assay was performed a described above, except for Assay A: The equilibration process (antibody/antigen mix) was performed in presence of an excess of one target (500 pM of either IL18 or I L- 1 ⁇ ) while assessing the KD of the other target. Assay B: The equilibration process (antibody/antigen mix) was performed with both targets in serial dilutions in one mix simultaneously (constant concentration of antibody 10 pM, highest antigen cone, see above). The same mix was then analyzed for its free antibody concentration on IL18 and I L-1 ⁇ coated plates as described above.
  • the SET Data were exported to Xlfit, an MS Excel add-in software. Average ECL- signals were calculated from duplicate measurements within each assay. Data were baseline adjusted by subtracting the lowest value from all data points and plotted against the corresponding antigen concentration to generate titration curves. KD values were determined by fitting the plot with the following:
  • KG-1 cells were grown in RPMI 1640 supplemented with 10% fetal bovine serum, 1 % L-Glutamine and 1 % penicillin/streptomycin at a density of 2x10 5 to 1x10 6 viable cells/mL.
  • HEK-BlueTM IL-18/IL-1 ⁇ cells were grown in Growth Medium (DMEM, 4.5 g/l glucose, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 mg/ml streptomycin, 100 mg/ml
  • HygroGoldTM and 100 g/ml of ZeocinTM are commercially available commercially.
  • PBMC peripheral blood mononuclear cells
  • the heparinized whole- blood samples were diluted with equal volumes of PBS, and 25 ml of the diluted blood was added to a LeucoSep tube.
  • the cell separation tubes were centrifuged for 15 min at 800 ⁇ g without break at room
  • the cell suspension layer was collected, and the cells were washed twice in PBS (for 10 min at 640 and 470 ⁇ g, respectively, for the two successive washes) and re-suspended in culture medium before counting.
  • IL-1 ⁇ induced IL-6 production assay in fibroblasts was conducted essentially as described (Gram 2000) with only minor modifications. Briefly, fibroblasts were seeded at a density of 5x103 cells per well (in 100 ⁇ ) in a 96 well flat bottom tissue culture plate. The following day, cells were starved for 5h in starving medium before addition of the recombinant IL- ⁇ /compound solution mix (I L- ⁇ concentration indicated in the table). The IL- ⁇ /compound solution mix was prepared beforehand by incubating recombinant IL-1 ⁇ with a concentration range of compound for 30min at 37°C.
  • the cell supernatants were collected after o/n incubation at 37°C and the amount of released IL-6 determined by ELISA.
  • the I L-1 ⁇ induced IL-6 production assay in PBMC was performed according to the following. PBMC were seeded at 3x10 5 cells per well (in 10 ⁇ ) in a 96 well tissue culture plate and incubated with a recombinant IL- ⁇ /compound solution mix for 24h at 37°C ( I L- 1 ⁇ concentration indicated in the table). The IL- ⁇ /compound solution mix was prepared beforehand by incubating recombinant IL-1 ⁇ with a concentration range of compound for 30min at 37°C. The cell supernatants were collected after 24h of stimulation and the amount of released IL-6 determined by ELISA. (f) IL-18 neutralization assays
  • the assay was conducted essentially according to the following. KG-1 cells (starved for 1 h in PBS + 1 % FCS beforehand) or PBMC at a density of 3 x10 5 per well were seeded into round bottom 96-well cell culture plates and incubated with a solution mix of recombinant I L-18/IL-12 together with a concentration range of compounds (IL- 18/IL-12 concentrations indicated in the table). After an incubation of 24h at 37°C, supernatants were collected and the amount of released IFNv determined by ELISA. For the assays with marmoset blood 85 ⁇ of blood per well were used.
  • the assay was conducted essentially as described in the manufacturer's handling procedures. Briefly, the HEK-BlueTM cells were seeded at a density of 4 x10 4 per well into 96-well cell culture plates and incubated with a solution mix of recombinant I L-1 ⁇ and IL- 18 (to produce a 1 :1 SEAP signal) together with a concentration range of compounds. After an incubation of 24h at 37°C, supernatants were collected and the amount of released SEAP determined by using the QUANTI-BlueTM method according to the manufacturer's instructions.
  • Binding affinities of bbmAbl to human and marmoset recombinant I L-1 ⁇ and IL- 18 proteins were measured by solution equilibrium titration (SET) titration ( Figure 5) and the KD values generated were compared to those of mAb2 for I L-1 ⁇ and mAbl for I L-18 binding.
  • Figure 5 shows titration curves of ECL-based affinity determination in solution, constant concentration of antibody: for IL-18 readout 10 pM, for I L-1 ⁇ readout 1 pM; antigen dilutions: highest cone: hulL-18, 5 nM; marlL-18, 10 nM; hulL-1 ⁇ , 0.5 nM; marlL- 1 ⁇ , 0.5 nM.
  • Solid lines represent a fit of the data using the model described above.
  • bbmAbI Comparing binding affinities in the individual target binding assay, bbmAbI showed a similar mean KD compared to mAbl for human and marmoset IL-18 (Table 7). For human I L-1 ⁇ binding the mean KD value was slightly higher for bbmAbI (2.6 pM) compared to mAb2 (0.6 pM) but still in the same low pM range. Subsequent
  • the neutralizing activity of bbmAbI on IL-1 ⁇ was assessed by the inhibition of recombinant IL- ⁇ -induced IL-6 production in human dermal fibroblasts (IL-1 ⁇ used at 6pM) and in human PBMC (I L-1 ⁇ used at 60pM).
  • the neutralizing activity of bbmAbI on IL-18 was measured by the inhibition of recombinant IL-18-induced IFN- ⁇ production in KG-1 cells and human PBMC (both cells activated with 3nM recombinant human IL-18 together with 1 ng/ml of recombinant human IL-12).
  • the inhibitory potency of bbmAbI on I L-1 ⁇ and IL-18 was always compared to that of either mAb2 or mAbl , respectively.
  • the mean IC50 values of bbmAbI were in sub-nM or single digit nM ranges and up to 2-to 4-fold higher in direct comparison mAb2 (for IL-1 ⁇ ) and mAbl (for IL-18), respectively (Table 9 and Table 10).
  • the monovalent format of bbmAbI as compared to the bivalent format of mAb2/mAb1 but also potentially the KiH mutations may be reasons for this slight difference in potency of bbmAbI .
  • bbmAbI was able to neutralize simultaneously the bioactivity of both I L-1 ⁇ and IL-18 as demonstrated with the HEK BlueTM reporter cells producing SEAP in response to a 1+1 stimulation with recombinant I L- 1 ⁇ and IL-18 (Table 11).
  • a similar inhibition of SEAP in this assay system was only achievable by the combination of mAb2 and mAbl but not by the use of the individual antibodies.
  • bbmAbl was shown to be fully cross -reactive to marmoset I L- 1 ⁇ and marmoset IL-18 in functional assays using marmoset responder cells.
  • bbmAbI a KiH format I L-1 ⁇ / ⁇ L-18 bi-specific mAb retains the high affinity binding as well as the cytokine neutralizing potency to the two individual targets I L- 1 ⁇ and IL-18 when compared to the original mAbs, mAb2 and mAbl , in a variety of different cell assays.
  • the dual I L-1 ⁇ and IL-18 neutralizing properties of bbmAbI were not only demonstrated for the human cytokines/cells but also for the corresponding marmoset cytokines/cells, facilitating appropriate toxicology studies.
  • IC50 values that were generated in some of the cellular assays for I L-1 ⁇ and IL-18 neutralization may be the consequence of the monovalent binding of bbmAbI as opposed to bi-valent binding of mAb2 and mAbl , respectively. Nevertheless, the dual cytokine neutralization by bbmAbI may result in additive or synergistic inhibitory activities in vivo that may not be adequately represented in our in vitro cellular systems.
  • Example 3 Effects of combined I L-1 ⁇ and IL-18 stimulation and blockade in PBMC Inflammasome activation-dependent cleavage of the effector cytokines I L-1 ⁇ and
  • IL-18 leads to the induction of secondary pro-inflammatory mediators and promotes immune cell recruitment/activation not only systemically but also at the site of
  • bbmAbI is a human/marmoset I L-1 ⁇ / ⁇ L-18 reactive bi-specific mAb with no rodent cross-reactivity and thus cannot be tested in mouse models.
  • LPS/IL-12 to mimic inflammasome-dependent pathway activation in vitro for the stimulation of human PBMC to reveal additive or synergistic inhibitory effects of combined I L-1 ⁇ / ⁇ L-18 neutralization by bbmAbI and performed a non-biased gene expression analysis using microarrays.
  • a complementary activity we also compared the gene expression profiles of PBMCs from different donors stimulated with either the combination of recombinant I L- 1 ⁇ and recombinant IL-18 or the single cytokines alone.
  • Recombinant human I L-18 was purchased from MBL (#B001-5)
  • I FNY ELISA MAX Standard Set, BioLegend, #430103 or BD OptEIA human I FNY ELISA Set, BD #555142
  • Chip priming station Agilent #5065- 4401
  • the tube was filled with 50mL of sterile PBS and centrifuged once at 1200rpm during 5min at RT. This centrifugation was repeated 2 times. The supernatant was gently discarded and cells re-suspended in 50mL of PBS with 2% FCS and 2mM EDTA. The cell suspension was filtered using a 70 ⁇ cell strainer and cells counted using trypan blue staining (500 ⁇ of trypan blue + 200 ⁇ of cells + 300 ⁇ _ of PBS).
  • LPS/IL-12 stimulation of PBMC Cytokine production in supernatants was prepared according to the following. 250 ⁇ 00 cells/well in 100ul final volume were distributed in 96-well round bottom plates. LPS was used at concentrations between 0.3ug/ml and 3000ug/ml together with recombinant IL-12 at 10ng/ml. Supernatants were harvested after 24h at 37°C and 10% C0 2 .
  • RNA extraction from cell pellets was performed according to the following. 3x10 6 cells/well in 1000ul final volume were distributed in flat bottom 24-well plates. LPS was used at 3ug/ml together with recombinant IL-12 at 10ng/ml. Cells were harvested after 24h at 37°C and 10% C0 2 .
  • PBMC peripheral blood mononuclear cells
  • recombinant cytokines 7x10 6 PBMC per well of a 12- well plate were used in 1.5ml final of complete RPMI medium.
  • Recombinant cytokines were added at the following final concentrations: 10ng/ml of recombinant I L- 1 , 3nM of recombinant IL-18, 1 ng/ml of recombinant IL-12. Both, supernatants as well as cells were collected after 4h and 24h at 37°C and 10% C0 2 .
  • RNA isolation, quantity and quality assessments Cells were pelleted and the pellet lysed in 350 ⁇ of Qiagen RTL buffer with 2% ⁇ -mercaptoethanol and frozen at - 20°C or -80°C until all samples of the study have been collected.
  • the RNA isolation was performed using the Qiagen standard protocol. Briefly, 350 ⁇ of 70% Ethanol was added in all samples prior to the transfer to the RNeasy spin column and centrifuged for 15s at 8000g. After discarding the flow-through, 350 ⁇ of buffer RW1 was added and the column centrifuged for 15s at 8000g to wash the spin column membrane.
  • DNase I incubation mix solution was prepared according to the manufacturer's instructions and added to the RNeasy spin column and incubated for 15min at RT. After washes with 350 ⁇ and 500 ⁇ of buffer RW1 , the RNeasy spin column was placed in new 2 ml collection tube and centrifuged at full speed for 1 min. RNA was finally collected by adding 35 ⁇ RNase-free water directly to the spin column membrane and a centrifugation for 1 min at 8000g to elute the RNA. The amount of RNA was measured using
  • Nanodrop ND-1000 and the RNA was stored at -20°C. RIN measurements were performed for the RNA quality assessment according to manufacturer's instructions. Briefly 1 ⁇ of RNA or ladder were pipetted into an Agilent RNA 6000 Nano chip and measured by using the Agilent 2100 Bioanalyser.
  • RNA/DNA free water was transferred into a 384-well reaction plate and then mixed with 1 ⁇ of 20X TaqMan® Gene Expression Assay target FAM gene and 10 ⁇ of 2x TaqMan® Gene Expression Master Mix and 10 ⁇ RNA/DNA free water.
  • the plate was loaded onto the Applied Biosystems ViiATM 7 Real-Time PCR System and the following instrument settings were used:
  • Microarrays was performed according to the following. Samples were processed by CiToxLAB France on Affymetrix HG_U 133_Plus2 microarrays. They were RMA normalized and analyzed in GeneSpring 1 1.5.1 (Agilent Technologies, Santa Clara, CA). Pathway analysis was done using Ingenuity Pathway Analysis (I PA) and Nextbio
  • DEG Differentially expressed genes
  • the respective signatures were used to calculate p-values with a Fisher's exact test which represent the statistical significance of observing an overlap between the signature and the 'disease gene list' (lesional vs non-lesional) of public datasets. To do so, the lists were uploaded into lllumina Base Space Correlation Engine (former Nextbio) and compared using the Meta-Analysis feature and keyword search for diseases.
  • bbmAb 1 is highly efficacious in inhibiting LPS/1 L- 12 induced IFNy production in whole blood
  • IL-12 Exposure of human whole blood to LP S supplemented with 10ng/ml IL-12 results in an IFNy response that is largely but not exclusively dependent on the "native" IL -18 produced by the blood cells.
  • IFNy is additively inhibited by bbmAb 1 (i.e. combined IL- 1 ⁇ / ⁇ -18 inhibition) compared to single IL- ⁇ or IL-18 inhibition in LPS/1 L-12 activated human PBMC
  • IL-26 is another pro-inflammatory cytokine additively inhibited by by bbmAbl in LPS/IL-12 stimulated PBMC
  • Figure 10 shows the inhibition of LPS (0.3ug/ml) /I L-12 induced IL-26 gene expression (by qPCR) ( Figure
  • I L-1 ⁇ /IL-l 8 differentially up- regulated genes (DEG) in PBMC (x-axis) compared to 5 different sarcoidosis tissue 'diseased vs healthy' DEG.
  • P- values represent the statistical significance of observing an overlap between the signature and the 'disease gene list'.
  • Black bar is skin from cutaneous sarcoidosis lesion vs skin from healthy patients.
  • Light grey bar is skin from cutaneous sarcoidosis lesion vs non-lesioned skin.
  • White bar is lacrimal glands from sarcoidosis patients vs normal.
  • Dark grey bar is anterior orbit tissues from sarcoidosis patients vs normal.
  • Striped bar is lung samples with progressive fibrotic, pulmonary sarcoidosis vs nodular self-limiting pulmonary sarcoidosis.
  • LPS and recombinant IL-12 was used to mimic pathogen associated molecular pattern (PAMP)-dependent NLRP3 inflammasome activation within the first 24h of in vitro culture. It was demonstrated that combined inhibition of I L-1 ⁇ and IL-18, by using bbmAbl , acts additively to decrease/inhibit IFNv production in PBMC stimulated with LPS/IL-12.
  • IL-12 was previously described to act synergistically with IL-18 to induce IFNv production in T, B, NK cells, macrophages and dendritic cells (as reviewed by Nakanishi, 2001) but now an additional stimulatory effect of I L- 1 ⁇ on IFNv production could be demonstrated under the experimental conditions used.
  • IL-26 a member of the IL-20 cytokine subfamily (I L-19, IL-20, IL-22, IL-24, and IL-26), which is conserved in most vertebrate species but absent in most rodent strains (including mice and rats) (Donnelly 2010).
  • IL-20R1 and IL-10R2 chains IL-20R1 and IL-10R2 chains.
  • IL-26 receptors are primarily expressed on non-hematopoietic cell types, particularly epithelial cells. Increased levels of IL-26 were reported in serum and particularly in the synovial fluid of RA patients (Corvaisier 2012) where it could act as factor to promote Th17 cell growth and differentiation.
  • Combined targeting of I L-1 ⁇ and IL-18 may represent a more effective treatment strategy than single cytokine blockade in inflammasome-driven inflammatory conditions where both innate and adaptive immunity components are involved.
  • Simultaneous neutralization of I L-1 ⁇ and IL-18 targets both innate and adaptive immunity components, including neutrophils, Th1/Tc1 and NK cells, adhesion molecules on immune and endothelial cells and pro-inflammatory cytokines (e.g. IL-6, IFNv and I L-17).
  • the overall clinical strategy for a bispecific which targets both I L-1 ⁇ and IL-18 simultaneously, may represent a more effective means of treatment than currently available options.
  • preclinical and translational research was utilized to demonstrate active involvement of both I L-1 ⁇ and IL-18 downstream pathways in the underlying pathophysiology of candidate diseases.
  • chronic pulmonary sarcoidosis is an inflammasome-driven disease with both innate and adaptive immunity involvement.
  • initial findings indicate important roles for both I L-1 ⁇ and IL-18 effector cytokines in this disease.
  • sarcoidosis represents an ideal opportunity to demonstrate the dual specificities of bbmAbl in a disease with established, chronic tissue inflammation.
  • Efficacy of bbmAbl in sarcoidosis may lead to development in other interstitial lung diseases such as hypersensitivity (occupational) lung diseases due to silica or beryllium.
  • Other candidate diseases are granulomatous inflammation involving other organ tissues, such as Crohn's disease.
  • vascular inflammation with tissue injury and endothelial dysfunction also represents a potential target for bbmAbl .
  • Dysfunctional endothelial cells can respond to effective anti-inflammatory treatment, resulting in improved vascular flow even in the presence of fixed intravascular defects.
  • Recent literature evidence has identified sickle cell disease (SCD) to have a strong inflammasome-driven component via high rates of constitutive intravascular hemolysis.
  • SCD sickle cell disease
  • danger signals uric acid, heme/Fe3+, other intracellular components
  • bbmAbl treatment could attenuate chronic background inflammation and prevent acute crises with associated end-organ damage, prevent acute sickle cell crises and associated end organ damage, along with improving patients' general quality of life by reducing associated chronic pain and fatigue.
  • Demonstration of bbmAbl therapeutic efficacy in SCD patients may lead to development in other chronic or acute on chronic inflammatory conditions involving high rates of hemolysis, such as malaria and hemodialysis- dependent, chronic kidney disease.
  • Further indications that might benefit from both I L-1 ⁇ and IL-18 modulation are indications associated with ischemia-reperfusion tissue injury, such as cardiovascular diseases or improved healing of wounds of all types, but in particular the most severe soft tissue injury of burn.
  • a method of treating an inflammasome related disorder comprising administering to a subject, afflicted with an inflammasome related disorder, an effective amount of a bbmAb disclosed herein, such as bbmAbl
  • a bbmAb disclosed herein such as bbmAbl
  • Potential inflammasome related disorders are cryopyrin-associated autoinflammatory syndrome (CAPS), familial Mediterranean fever (FMF), systemic juvenile idiopathic arthritis (SJIA), lupus nephritis, diabetic nephropathy, acute kidney injury, renal hypertension, IgA nephropathy, glomerulonephritis (GN), frontotemporal dementia (FTD), Alzheimer's disease (AD), epilepsy, stroke, Parkinson's disease (PD), depression, sarcoidosis, such as pulmonary sarcoidosis, pancreatitis, idiopathic pulmonary fibrosis (IPF), non-alcoholic steatohepatitis (NAS
  • a method of treating sickle cell disease, vasculopathy, ischemia-reperfusion injury, cardiovascular disease, peripheral artery disease, atherosclerosis, vascular dysfunction, skeletal muscle ischemia, pulmonary sarcoidosis, fibrosis, malaria, hemodialysis-dependent, chronic kidney disease or Crohn's disease in a subject is provided, by administering an effective amount of a bbmAb disclosed herein, such as bbmAbl , to the subject.
  • compositions comprising the bbmAb antibodies, such as bbmAbl , formulated together with a pharmaceutically acceptable carrier.
  • the compositions can additionally contain one or more other therapeutic agents that are suitable for treating a medical condition.
  • Pharmaceutically acceptable carriers enhance or stabilize the composition, or can be used to facilitate preparation of the composition.
  • Pharmaceutically acceptable carriers include solvents, dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents, and the like that are physiologically compatible.
  • a pharmaceutical composition described herein can be administered by a variety of methods known in the art.
  • the route and/or mode of administration vary depending upon the desired results. It is preferred that administration be intravitreal, intravenous, intramuscular, intraperitoneal, or subcutaneous, or administered proximal to the site of the target.
  • the pharmaceutically acceptable carrier should be suitable for intravitreal, intravenous, intramuscular, subcutaneous, parenteral, spinal or epidermal administration (e.g., by injection or infusion).
  • the active compound i.e. bbmAb
  • the composition should be sterile and fluid. Proper fluidity can be maintained, for example, by use of coating such as lecithin, by maintenance of required particle size in the case of dispersion and by use of surfactants. In many cases, it is preferable to include isotonic agents, for example, sugars, polyalcohols such as mannitol or sorbitol, and sodium chloride in the composition. Long-term absorption of the injectable compositions can be brought about by including in the composition an agent which delays absorption, for example, aluminum monostearate or gelatin.
  • compositions described herein can be prepared in accordance with methods well known and routinely practiced in the art. See, e.g., Remington: The Science and Practice of Pharmacy, Mack Publishing Co., 20th ed., 2000; and Sustained and Controlled Release Drug Delivery Systems, J.R. Robinson, ed., Marcel Dekker, Inc., New York, 1978. Pharmaceutical compositions are preferably manufactured under GMP conditions. Typically, a therapeutically effective dose or efficacious dose of the bbmAb is employed in the pharmaceutical compositions described herein. The bbmAbs are formulated into pharmaceutically acceptable dosage forms by conventional methods known to those of skill in the art. Dosage regimens are adjusted to provide the optimum desired response (e.g., a therapeutic response).
  • Dosage unit form refers to physically discrete units suited as unitary dosages for the subjects to be treated; each unit contains a predetermined quantity of active compound calculated to produce the desired therapeutic effect in association with the required pharmaceutical carrier.
  • Actual dosage levels of the active ingredients in the pharmaceutical compositions described herein can be varied so as to obtain an amount of the active ingredient which is effective to achieve the desired therapeutic response for a particular patient, composition, and mode of administration, without being toxic to the patient.
  • the selected dosage level depends upon a variety of pharmacokinetic factors including the activity of the particular compositions described herein employed, or the ester, salt or amide thereof, the route of administration, the time of administration, the rate of excretion of the particular compound being employed, the duration of the treatment, other drugs, compounds and/or materials used in combination with the particular compositions employed, the age, sex, weight, condition, general health and prior medical history of the patient being treated, and like factors.
  • a physician or veterinarian can start doses of the antibodies described herein employed in the pharmaceutical composition at levels lower than that required to achieve the desired therapeutic effect and gradually increase the dosage until the desired effect is achieved.
  • effective doses of the compositions described herein, for the treatment of a wasting disorders described herein vary depending upon many different factors, including means of administration, target site, physiological state of the patient, whether the patient is human or an animal, other medications administered, and whether treatment is prophylactic or therapeutic. Treatment dosages need to be titrated to optimize safety and efficacy.
  • the dosage ranges from about 0.0001 to 100 mg/kg, and more usually 0.01 to 15 mg/kg, of the host body weight.
  • the dosage may range from 0.1 mg/eye to 5 mg/eye.
  • An exemplary treatment regime entails systemic administration once per every two weeks or once a month or once every 3 to 6 months.
  • An exemplary treatment regime entails systemic administration once per every two weeks or once a month or once every 3 to 6 months, or as needed (PRN).
  • Biotherapeutics such as bbmAbl
  • Biological therapeutics are usually administered on multiple occasions. Intervals between single dosages can be weekly, monthly or yearly.
  • Intervals can also be irregular as indicated by measuring blood levels of bbmAbl in the patient.
  • dosing intervals can be determined by a physician and administered monthly or as necessary to be efficacious.
  • dosage is adjusted to achieve a plasma antibody concentration of 1-1000 ⁇ g/ml and in some methods 25-500 ⁇ g/ml.
  • antibody can be administered as a sustained release formulation, in which case less frequent administration is required. Dosage and frequency vary depending on the half-life of the antibody in the patient. In general, human antibodies show longer half-life than that of chimeric antibodies and nonhuman antibodies. The dosage and frequency of administration can vary depending on whether the treatment is prophylactic or therapeutic.
  • a relatively low dosage is administered at relatively infrequent intervals over a long period of time. Some patients continue to receive treatment for the rest of their lives.
  • a relatively high dosage at relatively short intervals is sometimes required until progression of the disease is reduced or terminated, and preferably until the patient shows partial or complete amelioration of symptoms of disease. Thereafter, the patient can be administered a prophylactic regime.
  • GTFKSYAISWVRQAPGQGLEWMGNIIP M TG QTYYAQ KF QG R VT I TA D ESTSTA Y MELSSLRSEDTAVYYCARAAYHPLVFD NWGQGTLVTVSS
  • GTFKSYAISWVRQAPGQGLEWMGNIIP M TG QTYYAQ KF QG R VT I TA D ESTSTA Y MELSSLRSEDTAVYYCARAAYHPLVFD
  • SEQ ID NO: 18 DNA VL GATATCGTCCTGACTCAGCCCCCTAG
  • SEQ ID NO: 28 DNA VH CAGGTGCAGCTGGTGGAGAGCGGCG
  • SEQ ID NO: 30 DNA Heavy CAGGTGCAGCTGGTGGAGAGCGGCGGCGGCG

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AU2018284303A1 (en) 2019-11-07
AR112419A1 (es) 2019-10-30
UY37758A (es) 2019-01-31
EA201992670A1 (ru) 2020-07-08
PE20200384A1 (es) 2020-02-24
MA49394A (fr) 2020-04-22
JOP20190283B1 (ar) 2023-09-17
CR20190558A (es) 2020-01-28
JOP20190283A1 (ar) 2019-12-05
JP7106234B2 (ja) 2022-07-26
US20190002589A1 (en) 2019-01-03
BR112019025904A2 (pt) 2020-06-30
CU20190100A7 (es) 2020-11-30
KR20200005746A (ko) 2020-01-16

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