EP1832336B1 - Dispositif de mélange de contenus de récipients de laboratoire - Google Patents

Dispositif de mélange de contenus de récipients de laboratoire Download PDF

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Publication number
EP1832336B1
EP1832336B1 EP07004433A EP07004433A EP1832336B1 EP 1832336 B1 EP1832336 B1 EP 1832336B1 EP 07004433 A EP07004433 A EP 07004433A EP 07004433 A EP07004433 A EP 07004433A EP 1832336 B1 EP1832336 B1 EP 1832336B1
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EP
European Patent Office
Prior art keywords
adapter
mixing
holder
accomodating
previous
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
EP07004433A
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German (de)
English (en)
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EP1832336A1 (fr
Inventor
Peter Schmidt
Manfred Ebers
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eppendorf SE
Original Assignee
Eppendorf SE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE102006011371A external-priority patent/DE102006011371A1/de
Application filed by Eppendorf SE filed Critical Eppendorf SE
Publication of EP1832336A1 publication Critical patent/EP1832336A1/fr
Application granted granted Critical
Publication of EP1832336B1 publication Critical patent/EP1832336B1/fr
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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/20—Mixing the contents of independent containers, e.g. test tubes
    • B01F31/201—Holders therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/20—Mixing the contents of independent containers, e.g. test tubes
    • B01F31/22—Mixing the contents of independent containers, e.g. test tubes with supporting means moving in a horizontal plane, e.g. describing an orbital path for moving the containers about an axis which intersects the receptacle axis at an angle
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
    • B01F2101/23—Mixing of laboratory samples e.g. in preparation of analysing or testing properties of materials
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
    • B01F23/40—Mixing liquids with liquids; Emulsifying
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/44—Mixers with shaking, oscillating, or vibrating mechanisms with stirrers performing an oscillatory, vibratory or shaking movement
    • B01F31/445—Mixers with shaking, oscillating, or vibrating mechanisms with stirrers performing an oscillatory, vibratory or shaking movement performing an oscillatory movement about an axis

Definitions

  • the present invention relates to a device for mixing of laboratory vessel contents with a receiving adapter with a holder for receiving laboratory vessels in removable blocks and a drive, by which the receiving adapter can be set in a mixing movement, which in a substantially horizontal, plane circular translational oscillating runs.
  • exchange blocks devices for receiving vessels that can also be used for temperature control
  • Such exchangeable blocks and also the reaction vessels can be standardized.
  • 0.2 ml, 0.5 ml, 1.5 ml and 2.0 mm reaction tubes - as well as suitable replacement blocks.
  • cryo vessels for falcon vessels (1.5 ml and 50 ml), for glass vessels and beakers, for microtiter plates (MTP), for deep well plates (DWP), for slides and for PCR Plates with 96 wells.
  • MTP microtiter plates
  • DWP deep well plates
  • PCR Plates for slides and for PCR Plates with 96 wells.
  • This list is not exhaustive, but it does indicate the wide variety of laboratory vessels for which the mixers should be suitable.
  • foot prints - namely the base structure of exchange blocks.
  • An example of this is the ANSI SBS-1, SBS-2, SBS-3 or SBS-4 (as of 2004).
  • a laboratory shaker with different adapters, for example for glass containers, test tubes or microtiter plates is from the EP 1393797 known.
  • Such mixers are driven at a rotational frequency of 200 rpm to 1,500 rpm.
  • the frequency is usually adjustable.
  • the present invention has for its object to provide a mixer of the type described above, which has a wider range of applications.
  • a mixing device for laboratory vessel contents is provided with a receiving adapter and a drive.
  • the receiving adapter has a holder which is suitable for receiving vessels. This should preferably mean that the vessels can be introduced into the holder of the receiving adapter in such a way that they do not free themselves of themselves during undisturbed operation during the mixing movement into which the receiving adapter can be displaced by means of the drive.
  • the holder of the receiving adapter preferably satisfies certain standards.
  • the drive of the mixing device according to the invention is able to set the recording adapter in a mixing movement, which is substantially circularly translationally oscillating in a plane.
  • a mixing movement according to the invention can be described in that two (imaginary) points of the receiving adapter perform a circular motion with substantially the same angular position, the same angular velocity and the same radius.
  • the mixing movement is in a horizontal plane - so that a billet taken therein mixed with its vertical reaction vessels.
  • the mixing device is characterized in that the receiving adapter has an externally accessible vortex structure, a vortex adapter.
  • a vortex adapter This is suitable to take a container mounted from the outside held, in particular a test tube positively in the mixing movement.
  • the vortex adapter has a paragraph structure. This is on a (possibly even imaginary) circumference arranged with at least one paragraph, which points to the center of the circumference.
  • the vortex adapter may have a trough whose edge represents the "circumference” according to the invention with the "center” according to the invention in the center of the trough. The trough edge thus forms here so to speak a single circumferential shoulder on the circumference.
  • the vortex adapter may also have at least three radial flanks sloping towards the center, which are preferably arranged uniformly on the circumference. So then three such edges are preferably arranged at 120 ° to each other, four such flanks below 90 ° and so on.
  • the vortex adapter can also have an elastic surface which can be impressed into a depression by a vessel held thereon with a certain contact force.
  • the vortex adapter may also have a slightly elastic surface and / or a surface adhering to smooth surfaces.
  • a surface according to the invention preferably adheres to glass, because test tubes or mixing flasks are commonly made of this material - or of a similar plastic, for which the surface may preferably also be suitable.
  • this elastic surface of elastomer, rubber, rubber, neoprene or the like - also possibly each coated.
  • the device according to the invention can advantageously be used both as a mixer and as a vortexer without two devices having to be provided, and in particular even without having to exchange any adapter on the device.
  • the drive of the mixing device is preferably adjusted so that a frequency of the mixing movement also results in the range of more than 2,000 rpm or even more than 3,000 rpm.
  • the radius of the mixing device is preferably less than 3 cm or even less than 2 cm - to name, for example, suitable mixing motion parameters for vortexing.
  • mixing programs can also be run by a memory-programmed controller of the mixer according to the invention, which are particularly suitable in a first situation for mixing an inserted alternating block with reaction vessels - and immediately thereafter in a next situation for vortexing a test tube.
  • DNA chains can be brought into an undesirable state by excessive energy supply, which can adversely affect further processing.
  • Other possible applications of a controllable energy supply by an optimizable combination of speed and stroke result, for example, for adaptive comminution of substances by introduced media such as spherical particles, which ensure optimal power transmission, for example, on soft substances.
  • a generic device for mixing is characterized in that the holder has at least one Federeinposition that holds a recorded exchange block, frictional and frictional.
  • the holder is preferably not actually form-fitting and in particular has no additional catch and no additional latch as a mechanical form-fitting elements.
  • This is the holder of the invention particularly for machines - but not least for daily manual insertion, where despite sometimes occasionally decreasing concentration in positioning an exchange block with the vessel contents to be mixed ultimately must be safely and accurately positioned in the mixing device.
  • Laboratory machines are known to allow, for example, rapid and reproducible pipetting and dispensing operations. In this case, otherwise manually performed feed processes are performed, for example, by a motor-driven arm with appropriate dosing.
  • the arm can be movable in all three spatial axes. For a precise dosage, a particularly accurate positioning of the plates and vessels is very advantageous. This also applies to additional devices that are to be used together with such machines - that is, for example, for mixers of the type according to the invention - which can be equipped automatically by such a laboratory machine with vessels and exchange blocks.
  • the figure shows a three-dimensional view of a mixing device according to the invention.
  • a mixing device 2 can be seen with an upper-side, frame-shaped receiving adapter 4, which has a holder 6, 8 for receiving change blocks.
  • the receiving adapter 4 holds the support structures 6 and 8 in the shape of a frame around a substantially rectangular inner space 10 , which corresponds to the "foot print" of an exchange block (not shown).
  • This rectangular area 10 is covered by a rectangular mat 10 , which has in its center a trough 12 with a wall-like edge elevation.
  • the mat 10 is removable for cleaning and is injection molded from EPDM or silicone rubber.
  • the receiving adapter 4 is set together with the trough 12 in its in a horizontal plane circular translationally oscillating mixing movement - and so puts a liquid in the Test tube for mixing in a vortex.
  • the drive (not shown) of the mixing device 2 stops automatically.
  • This automatic starting and stopping of the vortexing which depends on the contact pressure, is possible according to the invention by means of a sensor which reacts to the contact pressure and turns on and off the drive of the mixer 2 .
  • This switching can also be controlled with a time delay in order, for example, not to take account of an accidental decrease in contact pressure-and also not to switch on the vortexer immediately, if it is touched accidentally, for example.
  • a support structures 6, 8 carries the frame-shaped receiving adapter 4 on the one hand three upside-down U-shaped spring clips 8, two of which are arranged on the receiving adapter frame 4 center- symmetrically along a longitudinal inner edge and one on a transverse inner edge in that from each of the spring clips 8 a resilient leg points into the interior of the frame 4 .
  • the receiving adapter 4 carries in each case opposite to its other two inner edges of three elastic abutment 6 each in the form of an elastomeric cylinder.
  • bracket 6 causes the Federeinposition 8 on a recorded change block (not shown) has a lateral holding force, which pushes it with its lower edge elastic in the elastic abutment 6 .
  • the Federeinpositionen 8 are not actually form-fitting, because they have, for example, no latching recess, in which, for example, a lower edge of a bill of exchange could engage positively. But by pushing in the lower edge of an inserted change block in the elastic abutment additionally creates a kind of positive connection.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Accessories For Mixers (AREA)
  • Mixers With Rotating Receptacles And Mixers With Vibration Mechanisms (AREA)
  • Devices For Use In Laboratory Experiments (AREA)

Claims (11)

  1. Dispositif de mélange de contenus de récipients de laboratoire comprenant
    - un adaptateur de réception (4) avec une fixation (6, 8) pour recevoir des récipients de laboratoire dans des thermoblocs interchangeables, et
    - un entraînement qui permet de déplacer l'adaptateur de réception (4) dans un mouvement de mélange qui suit, essentiellement dans un plan horizontal, un mouvement de translation circulaire et oscillant,
    caractérisé en ce que
    l'adaptateur de réception (4) présente une structure accessible de l'extérieur avec
    une structure de décrochement sur une circonférence avec au moins un décrochement qui est orienté vers le centre de la circonférence, à des fins d'entraînement par ajustement géométrique d'un récipient de laboratoire retenu posé de l'extérieur dans le mouvement de mélange.
  2. Dispositif selon la revendication précédente, caractérisé en ce que la structure accessible de l'extérieur présente
    une cavité (12) et/ou
    au moins trois flancs radiaux inclinés vers le centre et/ou
    une surface pouvant être enfoncée élastiquement vers une cavité (12).
  3. Dispositif selon une des revendications précédentes, caractérisé en ce qu'au moins la structure de décrochement de la structure accessible de l'extérieur présente une surface élastique et/ou une surface adhérant sur des surfaces lisses.
  4. Dispositif selon une des revendications précédentes, caractérisé en ce que la structure accessible de l'extérieur peut être retirée de l'adaptateur de réception (4), en particulier une partie de la surface supérieure d'une natte interchangeable (10) sur l'adaptateur de réception (4).
  5. Dispositif selon une des revendications précédentes, caractérisé en ce que l'adaptateur de réception (4) peut être déplacé par l'entraînement dans un mouvement de mélange à une fréquence de plus de 2000 tr/min. et/ou à un rayon de moins de 3 cm.
  6. Dispositif selon une des revendications précédentes, caractérisé en ce que l'adaptateur de réception (4) peut être déplacé par l'entraînement dans un mouvement de mélange dont la fréquence et/ou le rayon sont ajustables.
  7. Dispositif selon une des revendications précédentes comprenant
    un adaptateur de réception (4) avec une fixation (6, 8) destinée à recevoir des récipients, en particulier des récipients de laboratoire dans des thermoblocs interchangeables, et
    un entraînement qui permet de déplacer l'adaptateur de réception (4) dans un mouvement de mélange qui suit, essentiellement dans un plan horizontal, un mouvement de translation circulaire et oscillant,
    caractérisé en ce que
    la fixation (6, 8) présente au moins un serrage à ressort (8) qui maintient par complémentarité de force et de frottement un thermobloc interchangeable contenu.
  8. Dispositif selon la revendication précédente, caractérisé en ce que la fixation (6, 8) n'est pas crabotée.
  9. Dispositif selon une des revendications 7 ou 8, caractérisé en ce que le serrage à ressort (8) exerce une résistance latérale sur un thermobloc interchangeable contenu.
  10. Dispositif selon une des revendications 7 à 9, caractérisé par un guidage qui introduit un bloc interchangeable devant être reçu, verticalement par le haut dans la fixation (6, 8) jusque contre une butée.
  11. Dispositif selon une des revendications 7 à 10, caractérisé par au moins un palier-support élastique (6), en particulier en forme de tuyau ou de cylindre élastique, dans lequel le serrage à ressort (8) enfonce un bloc interchangeable contenu.
EP07004433A 2006-03-09 2007-03-05 Dispositif de mélange de contenus de récipients de laboratoire Active EP1832336B1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE102006011371A DE102006011371A1 (de) 2006-03-09 2006-03-09 Vorrichtung zum Mischen insbesondere von Laborgefäß-Inhalten

Publications (2)

Publication Number Publication Date
EP1832336A1 EP1832336A1 (fr) 2007-09-12
EP1832336B1 true EP1832336B1 (fr) 2012-08-01

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Family Applications (1)

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EP07004433A Active EP1832336B1 (fr) 2006-03-09 2007-03-05 Dispositif de mélange de contenus de récipients de laboratoire

Country Status (5)

Country Link
US (1) US8550696B2 (fr)
EP (1) EP1832336B1 (fr)
JP (1) JP5114076B2 (fr)
CN (1) CN101053794A (fr)
DE (1) DE102006062714B4 (fr)

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CN108786611A (zh) * 2018-06-26 2018-11-13 广东腾湃医疗股份有限公司 一种减震效果优异的漩涡振荡器

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US20070212265A1 (en) 2007-09-13
DE102006062714B4 (de) 2013-02-21
US8550696B2 (en) 2013-10-08
CN101053794A (zh) 2007-10-17
DE102006062714A1 (de) 2007-10-04

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