EP1799349A1 - Dispositif destine a pipetter - Google Patents

Dispositif destine a pipetter

Info

Publication number
EP1799349A1
EP1799349A1 EP05797609A EP05797609A EP1799349A1 EP 1799349 A1 EP1799349 A1 EP 1799349A1 EP 05797609 A EP05797609 A EP 05797609A EP 05797609 A EP05797609 A EP 05797609A EP 1799349 A1 EP1799349 A1 EP 1799349A1
Authority
EP
European Patent Office
Prior art keywords
pipette
coupling
opening
discharge opening
main part
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP05797609A
Other languages
German (de)
English (en)
Inventor
Robert Hecht
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Beckman Coulter Inc
Original Assignee
Olympus Life and Material Science Europa GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Olympus Life and Material Science Europa GmbH filed Critical Olympus Life and Material Science Europa GmbH
Publication of EP1799349A1 publication Critical patent/EP1799349A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/26—Constructional details, e.g. recesses, hinges flexible
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance

Definitions

  • the invention relates to a device according to the preamble of claim 1.
  • FIG. 1 Another example is applicators for Impfifen on Nährbö ⁇ the. These are used to detect germs present in the seed, such as bacteria or fungi, by propagation by incubation.
  • the seed solution must be applied to the surface of a nutrient medium, usually in a Petri dish, in a defined application thickness.
  • the discharge opening of the pipette used for application must be very narrow.
  • the US Pat. No. 5,294,325 A shows in FIG. 1 a pipetting device in which a pipette in the form of a syringe needle can be attached to a pressure-generating device in the form of a syringe.
  • the liquid to be dispensed is taken up in the syringe in this construction.
  • the pipette absorbs the entire liquid to be dispensed, so that the liquid does not come into contact with the pressure-generating device. Therefore, the pipette may be formed as a disposable while the non-contaminated pressure generating means is reused.
  • the object of the present invention is to design a generic device with a higher operating speed.
  • an end piece of the pipette with the discharge opening can be coupled to a coupling opening of a main part of the pipette.
  • the pipette When coupled, the pipette can be used in a conventional manner for applying liquid.
  • the main part of the pipette through the much larger coupling opening can be filled very quickly by the usual suction. This makes it possible to achieve a considerably greater speed of operation, especially in mass examinations.
  • the coupling can be designed in a largely arbitrary manner, for example as a screw coupling or the like.
  • the features of claim 2 are provided.
  • Conical plug couplings form a simple and precise coupling which is largely customary in this technical field.
  • the end of the pipette may be rigid in a known manner.
  • it is elongated and flexurally flexible.
  • it can be applied touching the surface of a nutrient medium, its flexibility compensating for damage and changes in distance.
  • This is particularly advantageous in devices in which the pipette relative to the surface of a nutrient medium is relatively moved for line-wise application. This results in a higher application precision and a lower risk of damage to the surface of the culture medium.
  • a flexible end piece may e.g. in tapered tapered manner for pipettes, however, is preferably formed aus ⁇ according to claim 4.
  • the use of a piece of tubing as an end piece allows a particularly simple construction in which the elasticity properties and the size of the discharge opening can be precisely selected by selecting the piece of tubing.
  • FIG. 1 is a plan view of a device according to the invention with Nährbo ⁇ the and pipette.
  • Figure 2 is a section along line 2-2 in Fig. 1.
  • FIG. 3 shows a side view of a rack with pipette main parts before their removal
  • Fig. 6 is a side view of a completely held pipette.
  • Figs. 1 and 2 show a device according to the invention for applying a Impfses on the surface 2 of a nutrient medium 1, which is arranged on the bottom 3 a of a Petri dish 3, which is formed from its bottom 3a and a peripheral edge 3b.
  • a Petri dish 3 still includes a lid which serves for the germ-proof closure during the subsequent incubation.
  • the Petri dish 3 stands with its base 3a on a turntable 4 of a drive system, not shown in the drawing, with which the Petri dish 3 and the nutrient medium 1 can be driven about a vertical axis 5 in the direction of the arrow 6.
  • Impfates 7 is to be applied to the surface 2 of the culture medium 1, which is kept ready in a pipette 8.
  • the pipette 8 is pressurized by its one end via a connection hose 9, for example with air, and brings at its other end through a narrow discharge opening 10, the Impfates 7 in a line 11, which lays on the surface 2 of the culture medium 1.
  • the pipette 8 is held by a holding arm 12 which is moved and adjusted by a drive direction, not shown, that he holds the pipette 8 in a defined height above the surface 2 of the culture medium 1 and during the rotation of the culture medium 1, the discharge opening 10 in Direction of the arrow 13 moves radially outward.
  • the line 11 of seed solution applied to the surface 2 of the nutrient medium 1 therefore forms, as shown in FIG. 1, a spiral line.
  • connection hose 9 is connected to a pressure generating device, not shown, which ensures the pressurization of the pipette for ejecting the injection solution 7.
  • the output can be variably controlled, e.g. so that the line 11 is formed from one end to the other end with ever smaller order to dilute the applied concentration of germs.
  • the pipette 8 spiral over the stationary culture medium 1 moves was ⁇ , or it can by appropriate drive training other forms for the line 11, eg several parallel straight lines can be chosen.
  • the pipette 8 has a holder 14, on which the holding arm 12 and the An ⁇ closing hose 9 attack.
  • This holder 14 can also be used as a pumping device for Be pressurized the pipette 8 may be formed, in which case would be provided instead of the An ⁇ circuit hose 9 electrical control lines for controlling the Pumpvorgan ⁇ ges.
  • the pipette 8 shown in FIGS. 1 and 2 consists essentially of a main part 15, which can accommodate with its larger internal volume, the entire amount applied to 7 Impfels.
  • the main part 15 is couplable to the holder 14 via a conventional type cone plug coupling.
  • the main part 15 is connected to an end piece 16 via a cone plug coupling, which is likewise designed in the usual way, and consists of a coupling piece 17 and a hose 18.
  • the tail can be coupled to the main part 15, where there the main part 15 has a coupling opening 19 which is substantially larger than the very narrow discharge opening 10 at the end of the end piece 16th
  • the end piece 16 of the pipette 8 may also be formed in the usual conical pointed shape, but should be flexurally flexible, which could be achieved, for example, by suitable material thickness or material selection.
  • the tail may also be formed integrally with the main part 15.
  • the pipette 8 can be brought into the position shown in FIG. 2, with the holding arm 12, as shown in FIG , In doing so, the hose piece 18 in the sheet resilient the surface 2 of the culture medium 1, so that the end portion of the tube piece 18 is parallel to the surface of the culture medium 1.
  • the end piece 16 from the main part 15 of the pipette 8 is removable, in the illustrated embodiment with a cone clutch, in place of other types of coupling are possible.
  • a rack 20 shown in FIG. 3 can be used, in which a plurality of empty main components 15 are set up.
  • the holder 14 of the pipette 18 can be brought to the holding arm 12 in position on the respective next main part 15 on the rack 20 and verkup ⁇ pelt after lowering this.
  • the holder 14 with coupled, still empty main body 15, as shown in FIG. 4 can be brought and lowered via a sample vessel 21 with the next injection solution 7 to be processed until the coupling opening 19 of the main part 15 dips.
  • the main part 15 is filled.
  • the filled main part 15 is brought and coupled by corresponding movement of the holding arm 12 via a rack 22 with end pieces 16 stored therein.
  • the holding arm 12 After lifting the holding arm 12 is the completely assembled and filled pipette 8 in the position shown in FIG. 6 and can now be brought by appropriate height and angle control on a Petri dish 3 with nutrient medium 1 in the working position shown in FIG. 1 and 2.
  • the pipette 8 is used in a device described in FIGS. 1 and 2 for applying a seed solution to the surface 2 of a nutrient medium 1.
  • the pipette 8 shown in Figure 6 can also be used for other purposes.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Clinical Laboratory Science (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

L'invention concerne un dispositif destiné à pipetter et comprenant une pipette (8) pouvant être enfermée dans un dispositif d'alimentation en pression (9). Cette pipette présente un orifice de prélèvement (10) et est destinée à recevoir dans son volume interne la totalité du liquide à prélever (7). L'invention est caractérisée en ce que l'orifice de prélèvement (10) se trouve à une extrémité (16) de la pipette (8), laquelle extrémité peut être couplée à un orifice de couplage (19) d'un élément principal (15) de la pipette (8), cet élément principal recevant la totalité du liquide à prélever (7). L'orifice de couplage (19) est plus grand que l'orifice de prélèvement (10).
EP05797609A 2004-10-16 2005-10-04 Dispositif destine a pipetter Withdrawn EP1799349A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102004050466A DE102004050466A1 (de) 2004-10-16 2004-10-16 Vorrichtung zum Pipettieren
PCT/EP2005/010666 WO2006040040A1 (fr) 2004-10-16 2005-10-04 Dispositif destine a pipetter

Publications (1)

Publication Number Publication Date
EP1799349A1 true EP1799349A1 (fr) 2007-06-27

Family

ID=35511962

Family Applications (1)

Application Number Title Priority Date Filing Date
EP05797609A Withdrawn EP1799349A1 (fr) 2004-10-16 2005-10-04 Dispositif destine a pipetter

Country Status (6)

Country Link
US (2) US20080089811A1 (fr)
EP (1) EP1799349A1 (fr)
JP (1) JP2008517255A (fr)
CN (1) CN101076406B (fr)
DE (1) DE102004050466A1 (fr)
WO (1) WO2006040040A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2946057B1 (fr) * 2009-06-02 2014-02-14 Interlab Dispositif d'approvisionnement d'un automate d'ensemencement et procede utilisant un tel automate.
CN103990503B (zh) * 2014-05-16 2015-09-09 临沂大学 一次性移液枪涂布枪头及其使用方法
CN111175086A (zh) * 2020-02-19 2020-05-19 苏州微湃医疗科技有限公司 一种用于微量液体计量和移液的滴瓶制作方法

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US529325A (en) * 1894-11-13 S peters co
US2634028A (en) 1947-12-31 1953-04-07 Bell Telephone Labor Inc Mercury dispenser
US2834028A (en) * 1957-04-09 1958-05-13 Glenn D Stanley Toilet stand for children
DK109159A (fr) 1964-03-20
DE1291142B (de) * 1964-04-25 1969-03-20 Eppendorf Geraetebau Netheler Vorrichtung zum exakten Abmessen und UEbertragen kleiner Fluessigkeitsmengen
DE7200535U (de) * 1972-01-07 1972-04-06 Bajorat H Messvorrichtung zur ermittlung der foerdermenge einer vergaser-beschleunigerpumpe
DE7518809U (de) * 1975-06-12 1978-09-14 Eppendorf Geraetebau Netheler & Hinz Gmbh, 2000 Hamburg Pipettenspitze
JPS5865950A (ja) * 1981-10-14 1983-04-19 Nippon Denso Co Ltd 内燃機関の制御方法
DD207154A1 (de) * 1982-05-04 1984-02-22 Joachim Volke Pipettenspitze fuer mikrolitervolumina und verfahren zu ihrer herstellung
US4596780A (en) * 1983-03-16 1986-06-24 Chemetrics, Inc. Process for sampling and diluting
US4596563A (en) * 1983-06-09 1986-06-24 Cordis Corporation Thin-walled multi-layered catheter having a fuseless tip
US4721660A (en) * 1986-04-18 1988-01-26 Rca Corporation Electrically regeneratable fuel cell
US4707337A (en) * 1986-08-11 1987-11-17 Multi-Technology, Inc. Medical micro pipette tips for difficult to reach places and related methods
US4721680A (en) * 1986-08-11 1988-01-26 Multi-Technology, Inc. Methods of using micro pipette tips
US5214968A (en) * 1992-01-06 1993-06-01 Drummond Scientific Company Pipet filling and discharge device
US5294325A (en) * 1992-07-09 1994-03-15 World Precision Instruments, Inc. Miniaturized fluid conveying device and methods of use thereof
DE4335863C1 (de) * 1993-10-21 1995-02-02 Eppendorf Geraetebau Netheler Kolbenhubpipette
DE4420900A1 (de) * 1994-06-15 1995-12-21 Boehringer Mannheim Gmbh Verfahren zur mehrmaligen Entnahme von Flüssigkeiten
US5811306A (en) * 1995-09-04 1998-09-22 Fuji Photo Film Co., Ltd. Liquid spotting method
US20010044609A1 (en) * 1998-11-06 2001-11-22 Daniel C. Bock Universal outlet for filter units
JP2000304754A (ja) * 1999-02-12 2000-11-02 Ortho Clinical Diagnostics Inc 液体を混合するための方法及び装置
USD438954S1 (en) * 1999-04-12 2001-03-13 Ernst Orsing Sucking nozzle for medical and dental use
US7452508B2 (en) * 2000-02-22 2008-11-18 Jacobs Merrit N Aspirating and mixing of liquids within a probe tip
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US7241420B2 (en) * 2002-08-05 2007-07-10 Palo Alto Research Center Incorporated Capillary-channel probes for liquid pickup, transportation and dispense using stressy metal

Non-Patent Citations (1)

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Title
See references of WO2006040040A1 *

Also Published As

Publication number Publication date
CN101076406B (zh) 2011-04-20
US20100096405A1 (en) 2010-04-22
WO2006040040A8 (fr) 2007-07-05
CN101076406A (zh) 2007-11-21
JP2008517255A (ja) 2008-05-22
DE102004050466A1 (de) 2006-04-20
US20080089811A1 (en) 2008-04-17
WO2006040040A1 (fr) 2006-04-20

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