EP0722136A2 - Agitateur thermostatisé - Google Patents

Agitateur thermostatisé Download PDF

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Publication number
EP0722136A2
EP0722136A2 EP96100107A EP96100107A EP0722136A2 EP 0722136 A2 EP0722136 A2 EP 0722136A2 EP 96100107 A EP96100107 A EP 96100107A EP 96100107 A EP96100107 A EP 96100107A EP 0722136 A2 EP0722136 A2 EP 0722136A2
Authority
EP
European Patent Office
Prior art keywords
metal block
shaker
block thermostat
sample
thermostat
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP96100107A
Other languages
German (de)
English (en)
Other versions
EP0722136A3 (fr
Inventor
Joachim Schulz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19512835A external-priority patent/DE19512835C2/de
Application filed by Individual filed Critical Individual
Publication of EP0722136A2 publication Critical patent/EP0722136A2/fr
Publication of EP0722136A3 publication Critical patent/EP0722136A3/en
Withdrawn legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/20—Mixing the contents of independent containers, e.g. test tubes
    • B01F31/27—Mixing the contents of independent containers, e.g. test tubes the vibrations being caused by electromagnets
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/20—Mixing the contents of independent containers, e.g. test tubes
    • B01F31/275—Mixing the contents of independent containers, e.g. test tubes with means for transporting test tubes to and from the stirring device

Definitions

  • the invention relates to a metal block thermostat combination with a shaker for use in laboratories for biochemical, biotechnological and medical research as well as for diagnosis and also for quality assurance of biotechnological productions, e.g. in the manufacture of pharmaceutical products.
  • the invention is used in the analysis of very small quantities, often in solution of volumes in the microliter range, such as in the implementation of enzymatically catalyzed synthesis and cleavage reactions of high molecular weight substances, e.g. DNA and proteins that require high temperature precision and gentle mixing of the reaction components, whereby damage to the sensitive enzyme molecules by shear forces must also be avoided.
  • high molecular weight substances e.g. DNA and proteins that require high temperature precision and gentle mixing of the reaction components, whereby damage to the sensitive enzyme molecules by shear forces must also be avoided.
  • the invention can also be used to carry out lengthy solution processes and reactions of salts and high-molecular organic substances and to carry out extractions in the microliter range, where slow, continuous mixing of solutions with different phase formation is important in order to achieve an increase in the yield.
  • the molecular forces in the sample vessel can be overcome from a certain limit, but the stronger, periodically successive movement impulses prevent defined, reproducible control of the undesired shear effect in the sample.
  • the problem of the invention is to create a shaker with a heated metal block for temperature control and for holding sample vessels, which enables gentle, gentle and effective mixing of sensitive high-molecular biochemical substances and, in particular, the creation of harmful shear forces on the molecules of the sample vessels Avoids substances.
  • a shaker metal block thermostat is designed as an electromechanically drivable linear shaker, the stroke and frequency and intensity of which are infinitely adjustable, as well as in time intervals, in single shock pulses and in the intensity of the single shock pulses by means of a known per se
  • the program circuit can be controlled manually or automatically and the sample container holder on or above the metal block thermostat with the sample containers is mechanically guided independently of the shaking mobility of the metal block thermostat and can be raised manually or by means of a program control known per se and locked in any stroke position.
  • the lifting height and also the temporal lifting sequence and the duration of the locking can be regulated.
  • the horizontal, linear single shock pulses can also be exerted on the metal block thermostats which are at a standstill and the sample vessel carrier is raised.
  • the bores for the sample vessels in the metal block thermostat of the linear shaker can be chamfered, rounded or bordered with an elastic plastic on their upper edge.
  • the metal block thermostat assumes a defined central position with respect to the raised sample vessel carrier, which can be elastically locked by at least one resilient element.
  • the electromechanical drive of the linear shaker can be a tapping mechanism, in particular for generating individual pulses that are infinitely adjustable in their intensity or their impact energy, the tapping mechanism of which hits the linearly movable and linearly sprung metal block thermostats or the sample vessel carrier with the lifting mechanism extended, in the same direction.
  • the advantages of the solution according to the invention are that gentle, gentle reaction impulses can be transferred to the sensitive high-molecular biochemical substances in the sample vessels by means of individual impulses which can be precisely defined in their impact energy.
  • the thermal shaker according to the invention thus makes it possible to mechanize or automate a manual flicking of each sample vessel with the fingers and also to make the impact energy reproducible at any time in a precisely defined manner. It is also advantageous that the mechanization of this flipping process means that the sample vessels are only raised for a very short time and only a very short distance, and not even completely out of the holes in the metal block thermostat with the sample vessel carrier.
  • the thermal shaker according to the invention is of compact design and is equipped with a control panel.
  • the compact design makes it easy to change the location of the device, e.g. into a laminar box for operation under sterile conditions.
  • the invention is explained in more detail below using an exemplary embodiment.
  • the metal block thermostat with sample vessel support and sample vessels of a thermal shaker is shown schematically with FIG. 1.
  • FIG. 2 shows in the drawing the metal block thermostat of a thermal shaker with a raised sample vessel carrier and sample vessels.
  • the metal block thermostat 3 is arranged shake-proof on the electromechanically driven guide plate 4 of the thermal shaker. In the metal block thermostat 3 there are bores conforming to the sample vessels 1, here for conical Eppendorf tups.
  • the metal block thermostat 3 assumes a certain central position fixed by the resilient elements 8, in which the holes in the metal block thermostat 3 are aligned with the openings for the sample vessels 1 in the sample vessel carrier 2.
  • the sample vessel carrier 2 Independently of the linearly horizontally movable guide plate 4 with the metal block thermostat 3 located thereon, the sample vessel carrier 2, which can be moved up and down by means of the sample vessel carrier lifting mechanism 7, is arranged above it.
  • the lifting mechanism 7 is designed such that the sample vessel carrier 2 rests on the metal block thermostat 3 in the fully lowered working state and is positively connected to it, the lifting mechanism 7 being uncoupled from the sample vessel carrier.
  • the lifting movements of the lifting mechanism 7 take place when the shaking mechanism 6 is at a standstill.
  • All movement sequences can be preselected by means of program switching and can be controlled automatically.
  • sample vessels 1 in the form of conical Eppendorf tubes are first introduced into the sample vessel carrier 2.
  • the sample vessel carrier 2 can be located outside the thermal shaker or can already be placed in the sample vessel carrier lifting mechanism 7 on or above the metal block thermostat 3.
  • the operating parameters such as temperature control, stroke, frequency and time intervals for the shaking process can now be selected by means of a program connection, and the shaking process can then be carried out.
  • the program sequence can also be selected such that the sample vessel carrier 2 with the sample vessels 1 is not completely lifted out of the metal block thermostat 3 and remains in such a position when the shaking mechanism 6 is in the middle position by means of the sample vessel lifting mechanism 7 / or is locked so that an annular gap 5 is formed between them and the bore in the metal block thermostat as a result of the conically shaped sample vessels 1.
  • a defined single shock pulse is then triggered on the metal block thermostat 3 directly or on the guide plate 4 via the shaking stroke mechanism 6 against the resilient element 8 so that the upper edge of the bore in the metal block thermostat 3 briefly touches or carries along the conical wall of the sample vessel 1 and thus realized a snap-on effect.

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  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Electromagnetism (AREA)
  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
EP96100107A 1995-01-12 1996-01-05 Agitateur thermostatisé Withdrawn EP0722136A2 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE19500654 1995-01-12
DE19500654 1995-01-12
DE19512835A DE19512835C2 (de) 1995-01-12 1995-04-06 Thermoschüttler
DE19512635 1995-04-06

Publications (2)

Publication Number Publication Date
EP0722136A2 true EP0722136A2 (fr) 1996-07-17
EP0722136A3 EP0722136A3 (fr) 1996-08-28

Family

ID=26011541

Family Applications (1)

Application Number Title Priority Date Filing Date
EP96100107A Withdrawn EP0722136A2 (fr) 1995-01-12 1996-01-05 Agitateur thermostatisé

Country Status (1)

Country Link
EP (1) EP0722136A2 (fr)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10243209A1 (de) * 2002-03-22 2003-10-02 Endress & Hauser Wetzer Gmbh Vorrichtung zur Kühlung eines Probennehmers
WO2004053446A1 (fr) * 2002-12-06 2004-06-24 Thermogenic Imaging Systemes de microcalorimetres grande capacite et procedes
US7276351B2 (en) 2003-09-10 2007-10-02 Seahorse Bioscience Method and device for measuring multiple physiological properties of cells
US8202702B2 (en) 2008-10-14 2012-06-19 Seahorse Bioscience Method and device for measuring extracellular acidification and oxygen consumption rate with higher precision
US8658349B2 (en) 2006-07-13 2014-02-25 Seahorse Bioscience Cell analysis apparatus and method
US10118177B2 (en) 2014-06-02 2018-11-06 Seahorse Bioscience Single column microplate system and carrier for analysis of biological samples
CN109621799A (zh) * 2018-12-18 2019-04-16 安徽国泰众信检测技术有限公司 一种用于旋涡振荡器的辅助装置

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104471052B (zh) 2012-11-13 2017-06-13 海马生物科学公司 用于基于控制介质流动的三维组织测量的装置和方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2504269A1 (de) * 1973-11-14 1976-01-22 Osmo Antero Suovaniemi Verfahren und vorrichtung zum genauen pipettieren kleiner fluessigkeitsmengen
US3944188A (en) * 1974-05-20 1976-03-16 Buchler Instruments Div. Of Searle Analytic Inc. Concentrating vortex shaker
US5112134A (en) * 1984-03-01 1992-05-12 Molecular Devices Corporation Single source multi-site photometric measurement system
US5229074A (en) * 1988-07-25 1993-07-20 Precision Systems, Inc. Automatic multiple-sample multiple-reagent chemical analyzer

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2504269A1 (de) * 1973-11-14 1976-01-22 Osmo Antero Suovaniemi Verfahren und vorrichtung zum genauen pipettieren kleiner fluessigkeitsmengen
US3944188A (en) * 1974-05-20 1976-03-16 Buchler Instruments Div. Of Searle Analytic Inc. Concentrating vortex shaker
US5112134A (en) * 1984-03-01 1992-05-12 Molecular Devices Corporation Single source multi-site photometric measurement system
US5229074A (en) * 1988-07-25 1993-07-20 Precision Systems, Inc. Automatic multiple-sample multiple-reagent chemical analyzer

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10243209A1 (de) * 2002-03-22 2003-10-02 Endress & Hauser Wetzer Gmbh Vorrichtung zur Kühlung eines Probennehmers
WO2004053446A1 (fr) * 2002-12-06 2004-06-24 Thermogenic Imaging Systemes de microcalorimetres grande capacite et procedes
US7276351B2 (en) 2003-09-10 2007-10-02 Seahorse Bioscience Method and device for measuring multiple physiological properties of cells
US7638321B2 (en) 2003-09-10 2009-12-29 Seahorse Bioscience, Inc. Method and device for measuring multiple physiological properties of cells
US7851201B2 (en) 2003-09-10 2010-12-14 Seahorse Bioscience, Inc. Method and device for measuring multiple physiological properties of cells
US8697431B2 (en) 2003-09-10 2014-04-15 Seahorse Bioscience, Inc. Method and device for measuring multiple physiological properties of cells
US8658349B2 (en) 2006-07-13 2014-02-25 Seahorse Bioscience Cell analysis apparatus and method
US10359418B2 (en) 2006-07-13 2019-07-23 Seahorse Bioscience Cell analysis apparatus and method
US8202702B2 (en) 2008-10-14 2012-06-19 Seahorse Bioscience Method and device for measuring extracellular acidification and oxygen consumption rate with higher precision
US10118177B2 (en) 2014-06-02 2018-11-06 Seahorse Bioscience Single column microplate system and carrier for analysis of biological samples
CN109621799A (zh) * 2018-12-18 2019-04-16 安徽国泰众信检测技术有限公司 一种用于旋涡振荡器的辅助装置

Also Published As

Publication number Publication date
EP0722136A3 (fr) 1996-08-28

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