EP1832336B1 - Device for mixing the contents of laboratory vessels - Google Patents

Device for mixing the contents of laboratory vessels Download PDF

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Publication number
EP1832336B1
EP1832336B1 EP07004433A EP07004433A EP1832336B1 EP 1832336 B1 EP1832336 B1 EP 1832336B1 EP 07004433 A EP07004433 A EP 07004433A EP 07004433 A EP07004433 A EP 07004433A EP 1832336 B1 EP1832336 B1 EP 1832336B1
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EP
European Patent Office
Prior art keywords
adapter
mixing
holder
accomodating
previous
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EP07004433A
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German (de)
French (fr)
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EP1832336A1 (en
Inventor
Peter Schmidt
Manfred Ebers
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Eppendorf SE
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Eppendorf SE
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Priority claimed from DE102006011371A external-priority patent/DE102006011371A1/en
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/20—Mixing the contents of independent containers, e.g. test tubes
    • B01F31/201—Holders therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/20—Mixing the contents of independent containers, e.g. test tubes
    • B01F31/22—Mixing the contents of independent containers, e.g. test tubes with supporting means moving in a horizontal plane, e.g. describing an orbital path for moving the containers about an axis which intersects the receptacle axis at an angle
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
    • B01F2101/23—Mixing of laboratory samples e.g. in preparation of analysing or testing properties of materials
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
    • B01F23/40—Mixing liquids with liquids; Emulsifying
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/44—Mixers with shaking, oscillating, or vibrating mechanisms with stirrers performing an oscillatory, vibratory or shaking movement
    • B01F31/445—Mixers with shaking, oscillating, or vibrating mechanisms with stirrers performing an oscillatory, vibratory or shaking movement performing an oscillatory movement about an axis

Definitions

  • the present invention relates to a device for mixing of laboratory vessel contents with a receiving adapter with a holder for receiving laboratory vessels in removable blocks and a drive, by which the receiving adapter can be set in a mixing movement, which in a substantially horizontal, plane circular translational oscillating runs.
  • exchange blocks devices for receiving vessels that can also be used for temperature control
  • Such exchangeable blocks and also the reaction vessels can be standardized.
  • 0.2 ml, 0.5 ml, 1.5 ml and 2.0 mm reaction tubes - as well as suitable replacement blocks.
  • cryo vessels for falcon vessels (1.5 ml and 50 ml), for glass vessels and beakers, for microtiter plates (MTP), for deep well plates (DWP), for slides and for PCR Plates with 96 wells.
  • MTP microtiter plates
  • DWP deep well plates
  • PCR Plates for slides and for PCR Plates with 96 wells.
  • This list is not exhaustive, but it does indicate the wide variety of laboratory vessels for which the mixers should be suitable.
  • foot prints - namely the base structure of exchange blocks.
  • An example of this is the ANSI SBS-1, SBS-2, SBS-3 or SBS-4 (as of 2004).
  • a laboratory shaker with different adapters, for example for glass containers, test tubes or microtiter plates is from the EP 1393797 known.
  • Such mixers are driven at a rotational frequency of 200 rpm to 1,500 rpm.
  • the frequency is usually adjustable.
  • the present invention has for its object to provide a mixer of the type described above, which has a wider range of applications.
  • a mixing device for laboratory vessel contents is provided with a receiving adapter and a drive.
  • the receiving adapter has a holder which is suitable for receiving vessels. This should preferably mean that the vessels can be introduced into the holder of the receiving adapter in such a way that they do not free themselves of themselves during undisturbed operation during the mixing movement into which the receiving adapter can be displaced by means of the drive.
  • the holder of the receiving adapter preferably satisfies certain standards.
  • the drive of the mixing device according to the invention is able to set the recording adapter in a mixing movement, which is substantially circularly translationally oscillating in a plane.
  • a mixing movement according to the invention can be described in that two (imaginary) points of the receiving adapter perform a circular motion with substantially the same angular position, the same angular velocity and the same radius.
  • the mixing movement is in a horizontal plane - so that a billet taken therein mixed with its vertical reaction vessels.
  • the mixing device is characterized in that the receiving adapter has an externally accessible vortex structure, a vortex adapter.
  • a vortex adapter This is suitable to take a container mounted from the outside held, in particular a test tube positively in the mixing movement.
  • the vortex adapter has a paragraph structure. This is on a (possibly even imaginary) circumference arranged with at least one paragraph, which points to the center of the circumference.
  • the vortex adapter may have a trough whose edge represents the "circumference” according to the invention with the "center” according to the invention in the center of the trough. The trough edge thus forms here so to speak a single circumferential shoulder on the circumference.
  • the vortex adapter may also have at least three radial flanks sloping towards the center, which are preferably arranged uniformly on the circumference. So then three such edges are preferably arranged at 120 ° to each other, four such flanks below 90 ° and so on.
  • the vortex adapter can also have an elastic surface which can be impressed into a depression by a vessel held thereon with a certain contact force.
  • the vortex adapter may also have a slightly elastic surface and / or a surface adhering to smooth surfaces.
  • a surface according to the invention preferably adheres to glass, because test tubes or mixing flasks are commonly made of this material - or of a similar plastic, for which the surface may preferably also be suitable.
  • this elastic surface of elastomer, rubber, rubber, neoprene or the like - also possibly each coated.
  • the device according to the invention can advantageously be used both as a mixer and as a vortexer without two devices having to be provided, and in particular even without having to exchange any adapter on the device.
  • the drive of the mixing device is preferably adjusted so that a frequency of the mixing movement also results in the range of more than 2,000 rpm or even more than 3,000 rpm.
  • the radius of the mixing device is preferably less than 3 cm or even less than 2 cm - to name, for example, suitable mixing motion parameters for vortexing.
  • mixing programs can also be run by a memory-programmed controller of the mixer according to the invention, which are particularly suitable in a first situation for mixing an inserted alternating block with reaction vessels - and immediately thereafter in a next situation for vortexing a test tube.
  • DNA chains can be brought into an undesirable state by excessive energy supply, which can adversely affect further processing.
  • Other possible applications of a controllable energy supply by an optimizable combination of speed and stroke result, for example, for adaptive comminution of substances by introduced media such as spherical particles, which ensure optimal power transmission, for example, on soft substances.
  • a generic device for mixing is characterized in that the holder has at least one Federeinposition that holds a recorded exchange block, frictional and frictional.
  • the holder is preferably not actually form-fitting and in particular has no additional catch and no additional latch as a mechanical form-fitting elements.
  • This is the holder of the invention particularly for machines - but not least for daily manual insertion, where despite sometimes occasionally decreasing concentration in positioning an exchange block with the vessel contents to be mixed ultimately must be safely and accurately positioned in the mixing device.
  • Laboratory machines are known to allow, for example, rapid and reproducible pipetting and dispensing operations. In this case, otherwise manually performed feed processes are performed, for example, by a motor-driven arm with appropriate dosing.
  • the arm can be movable in all three spatial axes. For a precise dosage, a particularly accurate positioning of the plates and vessels is very advantageous. This also applies to additional devices that are to be used together with such machines - that is, for example, for mixers of the type according to the invention - which can be equipped automatically by such a laboratory machine with vessels and exchange blocks.
  • the figure shows a three-dimensional view of a mixing device according to the invention.
  • a mixing device 2 can be seen with an upper-side, frame-shaped receiving adapter 4, which has a holder 6, 8 for receiving change blocks.
  • the receiving adapter 4 holds the support structures 6 and 8 in the shape of a frame around a substantially rectangular inner space 10 , which corresponds to the "foot print" of an exchange block (not shown).
  • This rectangular area 10 is covered by a rectangular mat 10 , which has in its center a trough 12 with a wall-like edge elevation.
  • the mat 10 is removable for cleaning and is injection molded from EPDM or silicone rubber.
  • the receiving adapter 4 is set together with the trough 12 in its in a horizontal plane circular translationally oscillating mixing movement - and so puts a liquid in the Test tube for mixing in a vortex.
  • the drive (not shown) of the mixing device 2 stops automatically.
  • This automatic starting and stopping of the vortexing which depends on the contact pressure, is possible according to the invention by means of a sensor which reacts to the contact pressure and turns on and off the drive of the mixer 2 .
  • This switching can also be controlled with a time delay in order, for example, not to take account of an accidental decrease in contact pressure-and also not to switch on the vortexer immediately, if it is touched accidentally, for example.
  • a support structures 6, 8 carries the frame-shaped receiving adapter 4 on the one hand three upside-down U-shaped spring clips 8, two of which are arranged on the receiving adapter frame 4 center- symmetrically along a longitudinal inner edge and one on a transverse inner edge in that from each of the spring clips 8 a resilient leg points into the interior of the frame 4 .
  • the receiving adapter 4 carries in each case opposite to its other two inner edges of three elastic abutment 6 each in the form of an elastomeric cylinder.
  • bracket 6 causes the Federeinposition 8 on a recorded change block (not shown) has a lateral holding force, which pushes it with its lower edge elastic in the elastic abutment 6 .
  • the Federeinpositionen 8 are not actually form-fitting, because they have, for example, no latching recess, in which, for example, a lower edge of a bill of exchange could engage positively. But by pushing in the lower edge of an inserted change block in the elastic abutment additionally creates a kind of positive connection.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Accessories For Mixers (AREA)
  • Mixers With Rotating Receptacles And Mixers With Vibration Mechanisms (AREA)
  • Devices For Use In Laboratory Experiments (AREA)

Description

Die vorliegende Erfindung betrifft eine Vorrichtung zum Mischen von Laborgefäß-Inhalten mit einem Aufnahmeadapter mit einer Halterung zum Aufnehmen von Laborgefäßen in Wechselblöcken und einem Antrieb, durch den sich der Aufnahmeadapter in eine Mischbewegung versetzen lässt, die im wesentlichen in einer, horizontalen, Ebene kreisförmig translatorisch oszillierend verläuft.The present invention relates to a device for mixing of laboratory vessel contents with a receiving adapter with a holder for receiving laboratory vessels in removable blocks and a drive, by which the receiving adapter can be set in a mixing movement, which in a substantially horizontal, plane circular translational oscillating runs.

Mischvorrichtungen, in denen Gefäßinhalte vermengt werden, sind hinlänglich bekannt. Insbesondere für Labore gibt es Mischer, die auch kleine Flüssigkeitsmengen dadurch mischen können, dass kleine Behälter in geeigneten Haltern, so genannten "Wechselblöcken" (Vorrichtungen zur Aufnahme von Gefäßen, die auch zur Temperierung verwendet werden können) auch in sehr großen Gruppen zwei-, drei- oder sogar vierstelliger Anzahl zusammengefasst sind. Solche Wechselblöcke und auch die Reaktionsgefäße können genormt sein. So gibt es zum Beispiel Reaktionsgefäße mit 0,2 ml, 0,5 ml, 1,5 ml und 2,0 mm Inhalt - sowie jeweils geeignete Wechselblöcke standarisiert dazu. Ferner gibt es zum Beispiel Wechselblöcke für Cryo-Gefäße, für Falcon-Gefäße (1,5 ml und 50 ml), für Glasgefäße und Bechergläser, für Microtiterplatten (MTP), für Deep Well Platten (DWP), für Slides und für PCR-Platten mit 96 wells. Diese Aufzählung ist nicht abschließend, deutet aber an, in welcher großen Vielfalt Laborgefäße existieren, für die die Mischer geeignet sein sollten. Zu diesem Zweck gibt es Standards und Normen der so genannten "Foot Prints" - nämlich der Sockelstruktur von Wechselblöcken. Beispielhaft sei hier die ANSI SBS-1, SBS-2, SBS-3 oder SBS-4 (Stand 2004) genannt. Ein Laborschüttler mit unterschiedlichen Adaptern beispielsweise für Glasgefäße, Reagenzgläser oder Microtiterplatten ist aus der EP 1393797 bekannt.Mixing devices in which container contents are mixed are well known. Especially for laboratories, there are mixers that can mix even small amounts of liquid by small containers in suitable holders, so-called "exchange blocks" (devices for receiving vessels that can also be used for temperature control) even in very large groups two-, three or even four-digit number are summarized. Such exchangeable blocks and also the reaction vessels can be standardized. There are, for example, 0.2 ml, 0.5 ml, 1.5 ml and 2.0 mm reaction tubes - as well as suitable replacement blocks. There are also interchangeable blocks for cryo vessels, for falcon vessels (1.5 ml and 50 ml), for glass vessels and beakers, for microtiter plates (MTP), for deep well plates (DWP), for slides and for PCR Plates with 96 wells. This list is not exhaustive, but it does indicate the wide variety of laboratory vessels for which the mixers should be suitable. For this purpose, there are standards and norms of so-called "foot prints" - namely the base structure of exchange blocks. An example of this is the ANSI SBS-1, SBS-2, SBS-3 or SBS-4 (as of 2004). A laboratory shaker with different adapters, for example for glass containers, test tubes or microtiter plates is from the EP 1393797 known.

Weil diese Wechselblöcke prinzipiell so aufgebaut sind, dass die Einzelgefäße von oben dort hinein gesteckt werden, hat sich für die bekannten Mischer eine kreisförmig translatorisch oszillierende Mischbewegung etabliert, die im wesentlichen in einer horizontalen Ebene abläuft. Zu diesem Zweck ist bei den bekannten Mischern in aller Regel ein elektromotorischer Umwuchtantrieb dafür zuständig, einen "Tisch" in diese kreisförmige Bewegung zu versetzen. Letzterer ist dazu in bekanntlich unterschiedlicher Weise gelagert: Bekannt ist zum Beispiel eine Lagerung in Linear-Wälzlagern (so genannten Kugelbüchsen) in den beiden horizontalen Richtungen, bekannt ist aber auch eine Filmscharnierlagerung. Alternativ gibt es auch elektromagnetische Lagerung oder Lagerung mit Piezo-Elementen, die jeweils auch gleich als Antrieb dienen können.Because these exchange blocks are basically constructed so that the individual vessels are inserted from above into it, a circular translatory oscillating mixing movement has been established for the known mixer, which runs essentially in a horizontal plane. For this purpose, in the known mixers usually an electric motor balancing drive is responsible to put a "table" in this circular movement. The latter is stored in known different ways: For example, a storage in linear bearings (so-called ball bushings) is known in the two horizontal directions, but is known also a film hinge storage. Alternatively, there is also electromagnetic storage or storage with piezo elements, which can also serve as the same drive.

Üblicherweise werden solche Mischer mit einer Drehfrequenz von 200 U/min bis 1.500 U/min angetrieben. Die Frequenz ist in aller Regel einstellbar.Typically, such mixers are driven at a rotational frequency of 200 rpm to 1,500 rpm. The frequency is usually adjustable.

Zwar sind zum Mischen der oben genannten mannigfaltigen Reaktionsgefäße in Wechselblöcken die bekannten Mischer durchaus geeignet, sich sehr vielseitig - zum Mischen sehr verschiedener Reaktionsgefäße - einsetzen zu lassen. Wegen der erforderlichen Befestigungsmimik zum Halten der Wechselblöcke aber ist ein Mischen anderer Gefäße abseits dieses Geometriestandards nur mit anderen, jeweils dafür geeigneten und eingerichteten Mischvorrichtungen möglich. Deshalb gibt es in den meisten Laboren neben einem Mischer der beschriebenen Art auch andere Geräte. So ist weit verbreitet zum Beispiel ein so genannter "Vortexer"- der ein darauf von Hand gehaltenes Gefäß an seinem Boden ergreift und in eine Drehbewegung mitnimmt, so dass sich in der Flüssigkeit in dem Gefäß ein Strudel bildet und sich diese Flüssigkeit durchmischt. Vortexer des Standes der Technik sind beispielsweise aus dem patent US 4555183 bekannt. Auch wird in der WO 2005/094977 ein Vortexer mit einem optischen Sensor beispielsweise einer Lichtschranke, offenbart, der mit dem Mischen eines Laborgefäßes beginnt sobald der Sensor ein Signal abgibt.Although the known mixers are quite suitable for mixing the above-mentioned manifold reaction vessels in exchangeable blocks, very versatile - for mixing very different reaction vessels - to use. Because of the required attachment mimic for holding the exchange blocks but a mixing of other vessels is outside of this geometry standards possible only with other, suitable and equipped mixing devices. Therefore, in most laboratories in addition to a mixer of the type described also other devices. For example, a so-called "vortexer" is widely used - which grips a vessel held thereon by hand at its bottom and takes it into a rotary motion, so that a vortex forms in the liquid in the vessel and this liquid mixes. For example, vortexers of the prior art are known from the patent US 4555183 known. Also, in the WO 2005/094977 a vortexer with an optical sensor, for example a light barrier, which begins with the mixing of a laboratory vessel as soon as the sensor emits a signal.

Ergänzend wird nach auf die WO 2007/012437 A1 (Stand der Technik nach Art. 54 (3) EPÜ) verwiesen.In addition, after on the WO 2007/012437 A1 (State of the art according to Art. 54 (3) EPC).

Der vorliegenden Erfindung liegt die Aufgabe zugrunde, einen Mischer der eingangs beschriebenen Art zu schaffen, der einen größeren Anwendungsbereich aufweist.The present invention has for its object to provide a mixer of the type described above, which has a wider range of applications.

Diese Aufgabe wird von einer Vorrichtung zum Mischen mit den Merkmalen des Anspruchs 1 gelöst. Bevorzugte Ausgestaltungen sind in den Unteransprüchen angegeben.This object is achieved by a device for mixing with the features of claim 1. Preferred embodiments are specified in the subclaims.

Erfindungsgemäß ist eine Mischvorrichtung für Laborgefäß-Inhalte mit einem Aufnahme-Adapter und einem Antrieb versehen. Der Aufnahme-Adapter weist eine Halterung auf, die geeignet ist, Gefäße aufzunehmen. Dies soll vorzugsweise heißen, dass die Gefäße sich in die Halterung des Aufnahme-Adapters so einbringen lassen, dass sie sich während der Mischbewegung, in die der Aufnahme-Adapter sich mittels des Antriebs versetzen lässt, in ungestörtem Betrieb nicht von allein befreien. Insbesondere für Laborgefäße in Wechselblöcken genügt die Halterung des Aufnahme-Adapters vorzugsweise bestimmten Standards.According to the invention, a mixing device for laboratory vessel contents is provided with a receiving adapter and a drive. The receiving adapter has a holder which is suitable for receiving vessels. This should preferably mean that the vessels can be introduced into the holder of the receiving adapter in such a way that they do not free themselves of themselves during undisturbed operation during the mixing movement into which the receiving adapter can be displaced by means of the drive. Especially for laboratory vessels in interchangeable blocks, the holder of the receiving adapter preferably satisfies certain standards.

Der Antrieb der erfindungsgemäßen Mischvorrichtung ist in der Lage, den Aufnahme-adapter in eine Mischbewegung zu versetzen, die im wesentlichen kreisförmig translatorisch oszillierend in einer Ebene verläuft. Mit anderen Worten kann eine solche erfindungsgemäße Mischbewegung dadurch beschrieben werden, dass zwei (gedachte) Punkte des Aufnahme-Adapters eine Kreisbewegung mit im wesentlichen gleicher Winkelposition, gleicher Winkelgeschwindigkeit und gleichem Radius ausführen. Vorzugsweise verläuft die Mischbewegung in einer horizontalen Ebene - so dass ein darin aufgenommener Wechselblock mit seinen senkrecht stehenden Reaktionsgefäßen gemischt wird.The drive of the mixing device according to the invention is able to set the recording adapter in a mixing movement, which is substantially circularly translationally oscillating in a plane. In other words, such a mixing movement according to the invention can be described in that two (imaginary) points of the receiving adapter perform a circular motion with substantially the same angular position, the same angular velocity and the same radius. Preferably, the mixing movement is in a horizontal plane - so that a billet taken therein mixed with its vertical reaction vessels.

Die erfindungsgemäße Mischvorrichtung ist dadurch gekennzeichnet, dass der Aufnahme-Adapter eine von außen zugängliche Vortex-Struktur, einen Vortex-Adapter, aufweist. Dieser ist geeignet, ein von außen aufgesetzt gehaltenes Gefäß, insbesondere ein Reagenzglas formschlüssig in die Mischbewegung mitzunehmen. Dazu weist der Vortex-Adapter eine Absatzstruktur auf. Diese ist auf einem (möglicherweise auch nur gedachten) Umfang angeordnet mit mindestens einem Absatz, der zum Zentrum des Umfanges weist. So kann der Vortex-Adapter zum Beispiel eine Mulde aufweisen, deren Rand den erfindungsgemäßen "Umfang" darstellt mit dem erfindungsgemäßen "Zentrum" in der Mitte der Mulde. Der Muldenrand bildet hier also sozusagen einen einzigen auf dem Umfang umlaufenden Absatz.The mixing device according to the invention is characterized in that the receiving adapter has an externally accessible vortex structure, a vortex adapter. This is suitable to take a container mounted from the outside held, in particular a test tube positively in the mixing movement. To do this, the vortex adapter has a paragraph structure. This is on a (possibly even imaginary) circumference arranged with at least one paragraph, which points to the center of the circumference. For example, the vortex adapter may have a trough whose edge represents the "circumference" according to the invention with the "center" according to the invention in the center of the trough. The trough edge thus forms here so to speak a single circumferential shoulder on the circumference.

Alternativ oder kumulativ kann der Vortex-Adapter auch mindestens drei radiale zu dem Zentrum abfallende Flanken aufweisen, die vorzugsweise gleichmäßig auf dem Umfang angeordnet sind. So sind dann drei solcher Flanken vorzugsweise unter 120° zueinander angeordnet, vier solcher Flanken unter 90° und so weiter.Alternatively or cumulatively, the vortex adapter may also have at least three radial flanks sloping towards the center, which are preferably arranged uniformly on the circumference. So then three such edges are preferably arranged at 120 ° to each other, four such flanks below 90 ° and so on.

Alternativ oder kumulativ kann der Vortex-Adapter auch eine elastische Oberfläche aufweisen, die sich durch ein darauf mit einer gewissen Anpresskraft gehaltenes Gefäß zu einer Mulde eindrücken lässt.Alternatively or cumulatively, the vortex adapter can also have an elastic surface which can be impressed into a depression by a vessel held thereon with a certain contact force.

Diese Möglichkeiten der Beschaffenheit der erfindungsgemäßen Vortex-Struktur unterstützen erfindungsgemäß die sichere Mitnahme eines auf die Struktur gehaltenen Gefäßes in die Mischbewegung.These possibilities of the nature of the vortex structure according to the invention support the safe entrainment of a vessel held on the structure into the mixing movement.

Zur weiteren Unterstützung dieses Effekts kann der Vortex-Adapter eine auch nur leicht elastische Oberfläche und/oder eine an glatten Oberflächen haftende Oberfläche aufweisen. Insbesondere an Glas haftet eine solche erfindungsgemäß mögliche Oberfläche vorzugsweise, denn Reagenzgläser oder Mischkolben bestehen verbreitet aus diesem Material - oder aus einem ähnlich beschaffenen Kunststoff, für den die Oberfläche vorzugsweise auch geeignet sein kann. Bevorzugt besteht diese elastische Oberfläche aus Elastomer, Gummi, Kautschuk, Neopren oder dergleichen - auch jeweils möglicherweise beschichtet.To further support this effect, the vortex adapter may also have a slightly elastic surface and / or a surface adhering to smooth surfaces. In particular, such a surface according to the invention preferably adheres to glass, because test tubes or mixing flasks are commonly made of this material - or of a similar plastic, for which the surface may preferably also be suitable. Preferably, this elastic surface of elastomer, rubber, rubber, neoprene or the like - also possibly each coated.

Insgesamt lässt sich die erfindungsgemäße Vorrichtung vorteilhafterweise sowohl als Mischer als auch als Vortexer nutzen, ohne dass zwei Geräte bereitgestellt werden müssten, und insbesondere sogar, ohne an der Vorrichtung irgendeinen Adapter tauschen zu müssen.Overall, the device according to the invention can advantageously be used both as a mixer and as a vortexer without two devices having to be provided, and in particular even without having to exchange any adapter on the device.

Erfindungsgemäß ist der Antrieb der Mischvorrichtung vorzugsweise so einzustellen, dass sich eine Frequenz der Mischbewegung auch im Bereich von über 2.000 U/min oder sogar über 3.000 U/min ergibt. Der Radius der Mischvorrichtung beträgt vorzugsweise weniger als 3 cm oder sogar weniger als 2 cm - um beispielhaft geeignete Mischbewegungsparameter zum Vortexen zu nennen. Besonders bevorzugt ist es aber, die Mischbewegungsfrequenz, und sogar auch auch den Radius der Mischbewegung veränderlich einstellbar zu gestalten. So lassen sich zum Beispiel auch durch eine speicherprogrammierte Steuerung des erfindungsgemäßen Mischers Mischprogramme fahren, die in einer ersten Situation zum Mischen eines eingesetzten Wechselblocks mit Reaktionsgefäßen - und gleich darauf in einer nächsten Situation zum Vortexen eines Reagenzglases - besonders geeignet sind.According to the invention, the drive of the mixing device is preferably adjusted so that a frequency of the mixing movement also results in the range of more than 2,000 rpm or even more than 3,000 rpm. The radius of the mixing device is preferably less than 3 cm or even less than 2 cm - to name, for example, suitable mixing motion parameters for vortexing. However, it is particularly preferable to make the mixing movement frequency, and even the radius of the mixing movement variable, adjustable. Thus, for example, mixing programs can also be run by a memory-programmed controller of the mixer according to the invention, which are particularly suitable in a first situation for mixing an inserted alternating block with reaction vessels - and immediately thereafter in a next situation for vortexing a test tube.

Durch die Variation der Drehzahl und des Hubes ergeben sich für verschiedene unterschiedliche Mischaufgaben besonders vorteilhafte Mischeigenschaften, die sich durch Variation nur eines Mischparameters nicht erreichen ließen. So ist zum Beispiel in typischen Anwendungen für das Lösungsverhalten von Feststoffen im pharmazeutischen Bereich ein jeweils bestimmtes Drehzahl-/Hubverhältnis optimal, nämlich beispielsweise beim Auflösen von Tabletten unter hoher Energiezufuhr. Oder es werden Produkte aus der Zentrifugation oder der Vakuumaufkonzentration im Labor in Form von Pellets weiter verarbeitet. Hierbei spielt eine schnelle und vollständige Resuspendierung - unter vorteilhafter Einstellbarkeit von Frequenz und Hub - eine große Rolle.Andererseits muss beim so genannten Soft-Vortexen auf eine besonders schonende Behandlung des eingebrachten Materials Rücksicht genommen werden - wiederum unter vorteilhafter Einstellbarkeit von Frequenz und Hub. So können zum Beispiel bestimmte DNA-Ketten durch zu hohe Energiezufuhr in einen unerwünschten Zustand gebracht werden, welcher die weitere Verarbeitung negativ beeinflussen kann. Andere Anwendungsmöglichkeiten einer steuerbaren Energiezufuhr durch eine optimierbare Kombination von Drehzahl und Hub ergeben sich zum Beispiel für adaptives Zerkleinern von Substanzen durch eingebrachte Medien wie zum Beispiel kugelförmige Partikel, welche eine optimale Kraftübertragung beispielsweise auch auf weiche Substanzen-gewährleisten.Due to the variation of the rotational speed and the stroke, mixing properties which are particularly advantageous for various different mixing tasks are obtained that could not be achieved by variation of only one mixing parameter. For example, in typical applications for the dissolution behavior of solids in the pharmaceutical sector, a particular speed / stroke ratio is optimal, namely, for example, in the dissolution of tablets with a high energy input. Or, products from centrifugation or vacuum concentration in the laboratory are further processed in the form of pellets. Here, a rapid and complete resuspension - with an advantageous adjustability of frequency and stroke - plays a major role. On the other hand, the so-called soft vortexing requires a particularly gentle Treatment of the material introduced consideration - again with an advantageous adjustability of frequency and stroke. Thus, for example, certain DNA chains can be brought into an undesirable state by excessive energy supply, which can adversely affect further processing. Other possible applications of a controllable energy supply by an optimizable combination of speed and stroke result, for example, for adaptive comminution of substances by introduced media such as spherical particles, which ensure optimal power transmission, for example, on soft substances.

Unter einem weiteren Aspekt der vorliegenden Erfindung ist eine gattungsgemäße Vorrichtung zum Mischen dadurch gekennzeichnet, dass die Halterung mindestens eine Federeinspannung aufweist, die einen aufgenommenen Wechselblock, kraft- und reibschlüssig hält. Dabei ist die Halterung vorzugsweise nicht eigentlich formschlüssig und weist insbesondere keine zusätzliche Raste und keinen zusätzlichen Riegel als mechanische Formschluss-Elemente auf. Dies eignet die erfindungsgemäße Halterung besonders für Automaten - aber nicht zuletzt auch für das tägliche manuelle Einführen, wo trotz vielleicht gelegentlich nachlassender Konzentration beim Positionieren ein Wechselblock mit den zu mischenden Gefäß-Inhalten letztlich sicher und genau positioniert in die Mischvorrichtung eingesetzt sein muss. Laborautomaten ermöglichen bekannterweise zum Beispiel zügige und reproduzierbare Pipettier- und Dispensiervorgänge. Dabei werden sonst manuell durchgeführte Beschickungsabläufe zum Beispiel durch einen motorisch angetriebenen Arm mit entsprechenden Dosierwerkzeugen durchgeführt. Der Arm kann in alle drei Raumachsen beweglich sein. Für eine genaue Dosierung ist eine besonders genaue Positionierung der Platten und Gefäße sehr vorteilhaft. Dies gilt auch für Zusatzgeräte, die mit solchen Automaten zusammen eingesetzt werden sollen - also zum Beispiel auch für Mischer der erfindungsgemäßen Art - der sich von einem derartigen Laborautomaten selbsttätig mit Gefäßen und Wechselblöcken bestücken lässt.In a further aspect of the present invention, a generic device for mixing is characterized in that the holder has at least one Federeinspannung that holds a recorded exchange block, frictional and frictional. In this case, the holder is preferably not actually form-fitting and in particular has no additional catch and no additional latch as a mechanical form-fitting elements. This is the holder of the invention particularly for machines - but not least for daily manual insertion, where despite sometimes occasionally decreasing concentration in positioning an exchange block with the vessel contents to be mixed ultimately must be safely and accurately positioned in the mixing device. Laboratory machines are known to allow, for example, rapid and reproducible pipetting and dispensing operations. In this case, otherwise manually performed feed processes are performed, for example, by a motor-driven arm with appropriate dosing. The arm can be movable in all three spatial axes. For a precise dosage, a particularly accurate positioning of the plates and vessels is very advantageous. This also applies to additional devices that are to be used together with such machines - that is, for example, for mixers of the type according to the invention - which can be equipped automatically by such a laboratory machine with vessels and exchange blocks.

Weitere Vorteile und Merkmale der erfindungsgemäßen Vorrichtung werden mit Bezug auf die beigefügte Figur, die ein Ausführungsbeispiel der erfindungsgemäßen Mischvorrichtung darstellt, beschrieben.Further advantages and features of the device according to the invention will be described with reference to the attached figure, which shows an embodiment of the mixing device according to the invention.

Die Figur zeigt eine räumliche Ansicht einer erfindungsgemäßen Mischvorrichtung.The figure shows a three-dimensional view of a mixing device according to the invention.

In der Figur ist eine Mischvorrichtung 2 erkennbar mit einem oberseitigen, rahmenförmigen Aufnahme-Adapter 4, der eine Halterung 6, 8 zum Aufnehmen von Wechselblöcken aufweist.In the figure, a mixing device 2 can be seen with an upper-side, frame-shaped receiving adapter 4, which has a holder 6, 8 for receiving change blocks.

Der Aufnahme-Adapter 4 hält die Halterungsstrukturen 6 und 8 rahmenförmig um einen im wesentlichen rechteckigen Innenraum 10 herum, welcher dem "Foot Print" eines Wechselblocks (nicht dargestellt) entspricht. Dieser rechteckige Bereich 10 ist von einer rechteckigen Matte 10 überdeckt, welche in ihrer Mitte eine Mulde 12 mit einer wallartigen Randerhöhung aufweist. Die Matte 10 ist zum Reinigen herausnehmbar und ist aus EPDM oder Silikongummi spritzgegossen.The receiving adapter 4 holds the support structures 6 and 8 in the shape of a frame around a substantially rectangular inner space 10 , which corresponds to the "foot print" of an exchange block (not shown). This rectangular area 10 is covered by a rectangular mat 10 , which has in its center a trough 12 with a wall-like edge elevation. The mat 10 is removable for cleaning and is injection molded from EPDM or silicone rubber.

Wird nun zum Vortexen ein Reagenzglas (nicht dargestellt) in die Mulde 12 gehalten, und mit sanften Anpressdruck angedrückt, setzt sich der Aufnahme-Adapter 4 mitsamt der Mulde 12 in seine in horizontaler Ebene kreisförmig translatorisch oszillierende Mischbewegung - und versetzt so eine Flüssigkeit in dem Reagenzglas zum Mischen in einen Strudel. Sobald von der bedienenden Person der Anpressdruck nachgelassen wird, stoppt der Antrieb (nicht dargestellt) der Mischvorrichtung 2 automatisch. Dieses selbsttätige vom Anpressdruck abhängige Beginnen und Enden des Vortexens ist erfindungsgemäß durch einen Sensor möglich, der auf den Anpressdruck reagiert und den Antrieb des Mischers 2 ein- und ausschaltet. Dieses Schalten kann auch zeitverzögert gesteuert sein, um zum Beispiel ein versehentliches Nachlassen des Anpressdruckes nicht zu berücksichtigen - und den Vortexer auch nicht gleich anzuschalten, wenn er zum Beispiel nur versehentlich berührt wird.If now a vortex a test tube (not shown) held in the trough 12 , and pressed with gentle contact pressure, the receiving adapter 4 is set together with the trough 12 in its in a horizontal plane circular translationally oscillating mixing movement - and so puts a liquid in the Test tube for mixing in a vortex. As soon as the operating force of the contact pressure is released, the drive (not shown) of the mixing device 2 stops automatically. This automatic starting and stopping of the vortexing, which depends on the contact pressure, is possible according to the invention by means of a sensor which reacts to the contact pressure and turns on and off the drive of the mixer 2 . This switching can also be controlled with a time delay in order, for example, not to take account of an accidental decrease in contact pressure-and also not to switch on the vortexer immediately, if it is touched accidentally, for example.

Als Halterungsstrukturen 6, 8 trägt der rahmenförmige Aufnahme-Adapter 4 einerseits drei auf den Kopf gestellt U-förmige Federspangen 8, von denen zwei am Aufnahme-Adapter-Rahmen 4 mittensymmetrisch entlang einer Längs-Innenflanke und eine an einer Quer-Innenflanke so angeordnet sind, dass von jeder der Federspangen 8 ein federnder Schenkel in den Innenraum des Rahmes 4 weist. Andererseits trägt der Aufnahme-Adapter 4 jeweils gegenüberliegend an seinen anderen beiden Innenflanken drei elastische Gegenlager 6 jeweils in Gestalt eines Elastomer-Zylinders.As a support structures 6, 8 carries the frame-shaped receiving adapter 4 on the one hand three upside-down U-shaped spring clips 8, two of which are arranged on the receiving adapter frame 4 center- symmetrically along a longitudinal inner edge and one on a transverse inner edge in that from each of the spring clips 8 a resilient leg points into the interior of the frame 4 . On the other hand, the receiving adapter 4 carries in each case opposite to its other two inner edges of three elastic abutment 6 each in the form of an elastomeric cylinder.

Wenn nun ein Wechselblock (nicht dargestellt) von oben in die Halterung 6, 8 des Aufnahmeadapters 4 eingeführt wird, leitet die Form der Innenflanken der Federspangen 8 und der elastischen Gegenlager 6 den Wechselblock passiv in die Halterung bis gegen einen Anschlag 14 hinein. Dort ist ein eingeführter Wechselblock dann im wesentlichen nur kraft- und reibschlüssig und insbesondere nicht auch formschlüssig, also vorzugsweise ohne zusätzliche Raste oder zusätzlichen Riegel gehalten.Now, if a change block (not shown) is inserted from above into the holder 6, 8 of the receiving adapter 4 , the shape of the inner edges of the spring clips 8 and the elastic abutment 6 passes the exchange block passively into the holder up against a stop 14 . There is an imported bill of exchange then essentially only force and frictional and in particular not also form-fitting, so preferably without additional catch or additional latch held.

Dies eignet die erfindungsgemäße Halterung besonders für Automaten - aber nicht zuletzt auch für das tägliche manuelle Einführen, wo trotz vielleicht gelegentlich nachlassender Konzentration beim Positionieren ein Wechselblock mit den zu mischenden Gefäß-Inhalten letztlich sicher und genau positioniert in die Mischvorrichtung eingesetzt sein muss.This is the holder of the invention particularly for machines - but not least for daily manual insertion, where despite sometimes occasionally decreasing concentration in positioning an exchange block with the vessel contents to be mixed ultimately must be safely and accurately positioned in the mixing device.

Bei der abgebildeten Halterung 6, 8 bewirkt die Federeinspannung 8 auf einen aufgenommen Wechselblock (nicht dargestellt) eine seitliche Haltekraft, die diesen mit seinem unteren Rand elastisch in die elastischen Gegenlager 6 hineindrückt. So sind zwar die Federeinspannungen 8 nicht eigentlich formschlüssig, denn sie weisen zum Beispiel keine Rastvertiefung auf, in die zum Beispiel ein unterer Rand eines Wechselblocks formschlüssig eingreifen könnte. Aber durch das Hineindrücken des unteren Randes eines eingesetzten Wechselblocks in das elastische Gegenlager entsteht zusätzlich eine gewissermaßen formschlüssige Verbindung.In the illustrated bracket 6, 8 causes the Federeinspannung 8 on a recorded change block (not shown) has a lateral holding force, which pushes it with its lower edge elastic in the elastic abutment 6 . Thus, although the Federeinspannungen 8 are not actually form-fitting, because they have, for example, no latching recess, in which, for example, a lower edge of a bill of exchange could engage positively. But by pushing in the lower edge of an inserted change block in the elastic abutment additionally creates a kind of positive connection.

Claims (11)

  1. Device for mixing the contents of laboratory vessels with
    - an accommodating adapter (4) with a holder (6, 8) for receiving laboratory vessels in exchangeable block modules and
    - a drive configured to put the accomodating adapter (4) into a mixing motion, that oscillates in a circular and translatory manner mainly in a horizontal plane,
    characterized in that
    - the accomodating adapter (4) has a structure accessible from the outside with
    - a shoulder structure on a periphery with at least one shoulder, which points to the center of the periphery, for a laboratory vessel to be held and placed from the outside such that it picks up the the mixing motion in a form-fitting manner.
  2. The device according to the aforementioned claim, characterized in that the structure accessible from the outside has
    - a cavity (12) and/or
    - at least three radial flanks dropping off radially to the center and/or
    - a surface elastically pressable into the cavity (12).
  3. The device according to one of the previous claims, characterized in that at least the shoulder structure of the structure accessible from the outside has an elastic surface and/or a surface sticking to smooth surfaces.
  4. The device according to one of the previous claims, characterized in that the structure accessible from the outside is removable from the accomodating adapter (4), in particular a part of the upper surface of a replaceable mat on the r accomodating adapter (4).
  5. The device according to one of the previous claims, characterized in that the the drive can put the accomodating adapter (4) into a mixing motion with a frequency of more than 2,000 rpm and/or with a radius of less than 3 cm.
  6. The device according to one of the previous claims, characterized in that the drive can put the accomodating adapter (4) into a mixing motion, whereby the frequency and/or the radius of mixing motion is adjustable.
  7. The device according to one of the previous claims with
    - an accommodating adapter (4) with a holder (6, 8) for receiving vessels, in particular laboratory vessels in exchangeable block modules and
    - a drive configured to put the accommodating adapter (4) into a mixing motion, that oscillates in a circular and translatory manner mainly in a horizontal plane,
    characterized in that
    - the holder (6, 8) has at least one spring clamp (8), which holds a received exchangeable block module in a force-fitting and frictionally engaged manner.
  8. The device according to the previous claims, characterized in that the holder (6, 8) is not form-fitting.
  9. The device according to one of claims 7 or 8, characterized in that the spring clamp (8) exerts a lateral holding force on the received exchangeable block module.
  10. The device according to one of claims 7 through 9, characterized by a guide configured to introduce the exchangeable block module to be received in the holder (6, 8) in a vertical manner from above until reaching a stop.
  11. The device according to one of claims 7 through 10, characterized by at least one elastic counter bearing (6), in particular one in the design ofan elastic tube or cylinder, into which the spring clamp (8)pushes received exchangeable block module.
EP07004433A 2006-03-09 2007-03-05 Device for mixing the contents of laboratory vessels Active EP1832336B1 (en)

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DE102006011371A DE102006011371A1 (en) 2006-03-09 2006-03-09 Device for mixing a laboratory container contents, comprises a reception adapter with a support for the reception of the containers in alternation block, and a drive, through which the reception adapter is moved into a mixing movement

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EP1832336A1 EP1832336A1 (en) 2007-09-12
EP1832336B1 true EP1832336B1 (en) 2012-08-01

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EP1832336A1 (en) 2007-09-12
JP2007237173A (en) 2007-09-20
US20070212265A1 (en) 2007-09-13
DE102006062714B4 (en) 2013-02-21
US8550696B2 (en) 2013-10-08
CN101053794A (en) 2007-10-17
DE102006062714A1 (en) 2007-10-04

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