EP2633915B1 - Pipette avec système de calibration - Google Patents

Pipette avec système de calibration Download PDF

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Publication number
EP2633915B1
EP2633915B1 EP13000207.4A EP13000207A EP2633915B1 EP 2633915 B1 EP2633915 B1 EP 2633915B1 EP 13000207 A EP13000207 A EP 13000207A EP 2633915 B1 EP2633915 B1 EP 2633915B1
Authority
EP
European Patent Office
Prior art keywords
displaceable
pipette
housing
screw element
lift
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
EP13000207.4A
Other languages
German (de)
English (en)
Other versions
EP2633915A3 (fr
EP2633915A2 (fr
Inventor
Jens Wilmer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eppendorf SE
Original Assignee
Eppendorf SE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eppendorf SE filed Critical Eppendorf SE
Priority to PL13000207T priority Critical patent/PL2633915T3/pl
Publication of EP2633915A2 publication Critical patent/EP2633915A2/fr
Publication of EP2633915A3 publication Critical patent/EP2633915A3/fr
Application granted granted Critical
Publication of EP2633915B1 publication Critical patent/EP2633915B1/fr
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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/0224—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type having mechanical means to set stroke length, e.g. movable stops
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • B01L3/0279—Interchangeable or disposable dispensing tips co-operating with positive ejection means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/141—Preventing contamination, tampering
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/148—Specific details about calibrations

Definitions

  • the invention relates to a pipette for exchangeable pipette tips.
  • Pipettes are used especially in the laboratory for dosing liquids.
  • a pipette tip with an upper opening is clamped on a seat of the pipette.
  • the seat is usually a conical or cylindrical projection relative to a housing of the pipette, on which a pipette tip can be clamped with the upper opening.
  • Bubble pipettes include an air displacement device communicating with the pipette tip through a hole in the seat.
  • the displacement device By means of the displacement device, an air cushion is displaced, so that liquid is sucked into the pipette tip and ejected therefrom.
  • the displacement device has a displacement chamber with a displaceable boundary.
  • the displacement device is usually a cylinder with a piston displaceable therein.
  • pipette tips are released from the seat after use and replaced with a fresh pipette tip. As a result, contamination can be avoided at subsequent dosages.
  • pipette tips have a discharge device for ejecting the pipette tips, which allow ejection by button operation without touching the pipette tips.
  • Single-use pipette tips are available inexpensively from plastic.
  • the displaceable boundary is coupled to a drive device which serves to displace the piston in the cylinder.
  • the drive device has a lifting rod, which with a stop element between an upper and a lower stop is displaceable. At the beginning of the suction of air into the displacement chamber, the stop element is located at the lower stop. At the beginning of the displacement of air from the cylinder, the stop element is located at the top stop.
  • the absorbed or dispensed amount of liquid depends on the stroke of the movable limit and thus from the stroke of the lifting rod.
  • the stroke volume of the displaceable boundary does not correspond exactly to the amount of liquid absorbed and dispensed. Since the air column lengthens slightly under the weight of the liquid, the stroke volume exceeds the liquid volume.
  • the deviation of stroke volume and liquid volume depends in particular on the density and viscosity of the liquid, the temperature, the air pressure and wetting effects. It is known, for example from the WO 03/033151 or US-A-3,827,305 To calibrate pipettes by adjusting the position of an upper stopper body to a specific dosing volume.
  • the position of the top stop is variable.
  • Known pipettes have an upper stop body in the form of a threaded spindle which is adjustable in a fixedly arranged in the housing spindle nut.
  • To adjust the threaded spindle adjusting devices are available, which are coupled with display devices for displaying the set metering volume in the form of a counter.
  • the DE 43 35 863 C1 describes a pipette in which the display devices for calibration can be decoupled from the setting devices.
  • factory calibration is carried out by the pipette manufacturer under standard conditions. This double-distilled water is pipetted at a temperature of 20 to 25 ° C and an air pressure of 1013 mbar. For pipetting under different conditions, the user must change the factory calibration. Changing the factory calibration and later retrieving the factory calibration is expensive.
  • the EP 1 743 701 B1 describes a pipette in which the lower stopper body is held in a holder. Between the lower stopper body and the holder, an overstroke spring is arranged. In addition, adjusting means for adjusting the position of the holder with respect to the cylinder is provided. A display device displays the respective position of the holder.
  • This pipette allows the user to easily calibrate, for example, when metering a liquid that has a different density or viscosity than double-distilled water, or when working at a different air pressure or temperature.
  • the user calibration is carried out exclusively by adjusting the lower stop body. The adjustment made in the factory calibration of the upper stop body and the display devices is not changed.
  • the display device displays the respective position of the lower stop body. The position taken by the lower stop body during factory calibration can be easily retrieved at any time.
  • the holder is screwed coaxially to the lifting rod in a fixedly arranged in the housing carrier.
  • a coaxially arranged to the lifting rod indicator ring with a bevel gear at the top is rotatably mounted in the carrier. From the indicator ring are upwards parallel to the lifting rod driving pins before, in the axial direction in holes in the bottom of the engage cup-shaped holder.
  • On the carrier a pinion is rotatably mounted, which meshes with the bevel gear and has a hexagon socket in one end face.
  • a tool is insertable through a hole in the housing in the tool engagement to rotate the pinion. In this case, the display ring is rotated, which rotates about the driving pins with the holder.
  • the calibration accuracy is impaired due to manufacturing tolerances and wear of the many components.
  • the production costs are high due to the many components.
  • the lifting rod is displaceable with the stop element between the upper stop body and the lower stop body.
  • the stroke of the lifting rod and the movable limit is limited and determines the dosing.
  • the lower stop body is over a Supports overstroke spring, so that overcoming the spring force of the overstroke spring an overstroke to blow out residual liquid from a pipette tip is possible.
  • the lower stop body is adjustable.
  • the displaceable screw element is displaceable on the fixed screw, which displaces the lower stop body in the axial direction of the lifting rod during the displacement. As a result, the stroke of the lifting rod and the movable limit is changed.
  • the displaceable screw is a concentric with the lifting rod arranged threaded ring with a thread on the circumference, the top having the teeth and is seated on the bottom of the lower stop body.
  • the screw ring can be accommodated to save space in the housing and can sit evenly with its lower edge on the lower stop body.
  • the overstroke spring bearing is arranged in the lifting body and the overstroke spring arranged in the lifting body between the lower stopper body and overstroke spring bearing.
  • over-stroke spring bearing and over-travel spring are accommodated to save space.
  • the lifting body additionally saves space as a receptacle and support for the overstroke spring bearing and the overstroke spring.
  • the upper stop body is fixedly arranged in the housing.
  • a pipette with adjustable metering volume of the upper stop body is arranged displaceably in the housing in the axial direction of the lifting rod.
  • the upper stopper body is a threaded spindle, which is screwed into a fixedly connected to the housing spindle nut and having an upper passageway through which the lifting rod is passed and / or is the lower Stopper body circular disk-shaped and has a lower passageway through which the lifting rod is extending therethrough.
  • the lifting body is fixedly connected at the top with the spindle nut. This allows a space-saving accommodation of the means for adjusting the dosing volume.
  • the lifting body serves as a receptacle and support for the mother, spindle and lifting rod, which is inserted into the upper through hole and is displaceable with the stop element between the upper and lower stop body.
  • the bearing of the drive gear is fixedly connected to the lifting body above the external thread.
  • the lifting body also serves as a carrier of the drive gear.
  • the lifting body When mounting the pipette, the lifting body can be equipped separately with the various components.
  • the populated lifting body is an assembly that is completely mountable in the housing.
  • the lifting body is connected by a snap connection with the housing.
  • the lifting body is firmly connected to the housing in a simple manner.
  • the snap connection for disassembly of the lifting body can be selectively resolved.
  • the drive gear and the displaceable screw element are bevel gears or crown wheels.
  • the screw ring on the outer circumference in the circumferential direction spaced apart markings and the housing has a window for reading a arranged under the window mark.
  • the markings serve to indicate to the user the respective setting of the displaceable screw element and thus of the lower stop body.
  • a special mark is present, indicating to the user that the lower stop body has assumed the position in which the factory calibration has been carried out. On both sides of this central mark there may be further markings indicating the deviation of the position of the lower stop body from the factory calibration in both directions.
  • the markings are arranged on the circumference of the screw ring on a helical line whose pitch corresponds to the pitch of the thread of the displaceable screw element. This ensures that the various markings are always visible at the same point in the window during rotation of the displaceable screw despite the associated axial change in position of the movable screw.
  • the drive gear has a tool engagement for rotating the drive gear and the housing has a hole through which the tool attack is accessible by means of a tool introduced from outside into the hole.
  • the user can make the user calibration specifically by applying a tool to the tool attack and turning the tool in different directions.
  • the tool attack is for example a slot, Phillips, Torx, hexagon socket or other polygon socket.
  • means are provided for limiting the twisting of the displaceable screw element, which prevent the highest tooth of the toothing from being released from the drive gearwheel.
  • the highest tooth of the axial toothing is the tooth which is arranged on the highest position of the helix along which the toothing of the displaceable screw element runs. The limitation prevents the toothing from being released from the drive gear.
  • the terms “up” and “down”, “above” and “below” and “horizontally” and “vertically” refer to an orientation of the pipette in which the housing is vertically aligned with the seat. In this orientation, a pipette tip mounted on the seat can be directed to an underlying vessel to aspirate or dispense liquid.
  • the pipette 1 has a rod-shaped, molded as a handle housing with an upper housing part 2 and a lower housing part 3.
  • the upper housing part 2 with all the parts included forms a drive unit and the lower housing part 3 with all parts contained a displacement unit.
  • an actuator 4 in the form of a cylindrical push button out.
  • the actuating element 4 is mounted axially displaceably and rotatably in the upper housing part 2.
  • the spindle nut 9 is fixedly connected to a lifting body 10 which is fixed in the upper housing part 2.
  • the lifting body 10 is substantially cylindrical and is a support for the spindle nut 9, the threaded spindle 8 screwed therein and the lifting rod 6 guided therein.
  • these and other components are preassembled on the lifting body 10 and the lifting body 10 equipped with the components is mounted in the upper housing part 2, so that it is firmly held in the upper housing part 2.
  • the lifting body 10 is locked to the upper housing part 2.
  • the upper housing part 2 can be equipped inside corresponding to the lifting body 10.
  • the threaded spindle 8 has a rotatably connected thereto Spindelmitcret 11 above.
  • the Spindelmitstream 11 has on the circumference a hexagon 12 with a central hole 13.
  • the hexagon 12 engages in a Innsensechskant 14 of the actuating element 4 a.
  • the actuator 4 is provided at the bottom with two diametrically opposed, radial projections 15, 16 which project outwardly.
  • radial projections 15, 16 are present.
  • the radial projections 15, 16 engage in axially extending grooves 17, 18 on the inside of a hollow cylindrical transmission part 19, which is rotatably mounted in the upper housing part 2.
  • the transmission member 19 has at the top of the outer circumference a sprocket 20 which meshes with a gear of a counter gear 21 which drives a plurality of juxtaposed on a horizontal axis counter wheels 22 of a counter 23.
  • the counter 23 is attached to the upper housing part.
  • the Zählwerks impart 22 each have digits from 0 to 9. Das - based on Fig. 1 - The last counter gear 22 is driven by the counter gear 21. The next one arranged counting wheels 22 are further rotated in each case by one digit when the behind arranged Zählwerksrad passes from 9 to 0.
  • a beaded collar 25 is arranged as a stop element on the lifting rod 6. The displacement of the lifting rod 6 upwards is limited by contact of the collar 25 at the lower end face 26 of the threaded spindle 8, which forms an upper stop body for the collar 25.
  • the lower stop member 27 is guided on the projections 29 in axially extending guide slots 30 of the lifting body 10. This is also in Fig. 3 shown. Upwards he is 30 until the projection of the projections 29 at the upper end of the guide slots 30 displaced.
  • a lifting spring 31 designed as a helical spring is arranged in the lifting body 10.
  • the overstroke spring bears against the lower side of the lower stop body 27.
  • Bottom supports the overstroke spring 31 is arranged on an upper part of the housing 2 and thus fixedly connected over-stroke spring bearing 32.
  • a drive element 34 in the form of a coaxial with the lifting rod 6 aligned sleeve is present in the lifting body 10.
  • the drive member 34 has an upper sleeve portion 35 and a lower sleeve portion 36, wherein the upper sleeve portion 35 has larger inner and outer diameter than the lower sleeve portion 36.
  • the lower sleeve portion 36 has a frusto-conical tip 37 at the bottom.
  • a radially outwardly projecting, further circumferential collar 38 is present at the upper edge of the upper sleeve portion 35.
  • the outer diameter of the upper sleeve portion 35 is smaller than the inner diameter of the through hole 33 of the overstroke spring bearing 32, so that the lower and the upper sleeve portion 35, 36 are insertable into the through hole 33.
  • the outer diameter of the further collar 38 exceeds the inner diameter of the through-hole 33, so that the drive element 34 can not completely pass through the through-hole 33.
  • the over-stroke spring bearing 32 forms an end stop and the further collar 38 an end stop element, which limit the displacement of the drive element 34 down.
  • a hollow cylindrical armature 39 is arranged from a ferromagnetic material above. Below this, a hollow cylindrical magnet 40 is arranged in the upper sleeve section 35. Below this is a pot 41 which receives the magnet 40.
  • the armature 39 has an interference fit in the upper sleeve portion 35.
  • the lifting rod 6 extends slidably through the central hole of the armature 39 therethrough. Below the armature, the lifting rod 6 has a needle-shaped portion 42 of reduced diameter.
  • the magnet 40 and the pot 41 are seated on the needle-shaped portion 42.
  • the magnet 40 and pot 41 are fixed on the needle-shaped portion 42, e.g. by having a press fit there.
  • the magnet 40 is supported at the top on a shoulder of the lifting rod from which the needle-shaped portion 42 extends.
  • the hollow cylindrical portion 47 of the lower housing part 3 is provided at the top of the outer circumference with other means for releasable connection 54, which are not described in more detail, which are adapted to the means for releasably connecting 46 of the upper housing part 2 to releasably connect the lower housing part 3 with the upper housing part 2.
  • Suitable means for releasably connecting 46, 54 of the upper housing part 2 and lower housing part 3 are in the DE 10 2004 003 434 B4 described. In this regard, reference is made to the DE 10 2004 003 434 B4 and US2005 / 155438 A1 , the content of which is hereby incorporated into this application.
  • the lower hollow cone section 49 has at the top a projection 55 projecting beyond the upper hollow cone section 48 in the lower housing part 3.
  • the lower hollow cone portion 49 has a connecting channel 56 which connects the upper end face of the extension 55 with the lower end face of the projection 50.
  • an arrangement of a cylinder 57 is arranged with a displaceable piston 58 therein.
  • the cylinder 57 is inserted with a lower portion in the connecting channel 56 and fixed therein by press-fitting or gluing.
  • the cylinder 57 is sealed off from the connecting channel 56 by means of an O-ring 59.
  • the lower housing part 3 has at the top a pot-shaped closure cap 65 with a cylindrical or conical jacket.
  • the bottom of the cap 65 is disposed above the piston plate 63 and has a central upper housing opening 67, through which the piston plate 63 is accessible from above.
  • the cap 65 has outwardly projecting protrusions 68 which are snapped into corresponding recesses 69 of the hollow cylindrical portion 47 of the housing base 3.
  • the bottom of the cap 65 limits the displacement of the piston 58 upwards.
  • a trained as a spiral spring piston spring 70 which is supported at the bottom of the extension 55 and the top of the underside of the piston plate 63, biases the piston 58 against the underside of the cap 65 before.
  • the pipette 1 has a discharge device 71.
  • the discharge device 71 comprises a discharge slide 72, which is arranged on the housing lower part 3.
  • the discharge slide 72 has a contour adapted to the contours of the hollow cylindrical section 47, the upper hollow taper section 49 and the lower hollow taper section 49.
  • Below it has an annular discharge end 73.
  • the discharge end 73 is in the position of the discharge slide 72 of Fig. 1 pushed as far as possible up to the lower housing part 3, so that the conical projection 50 is free for attaching a pipette tip 51.
  • the webs 79 form a lower Abschfederlager 80.
  • a designed as a helical spring ejection spring 81 is under bias between the upper Abschfederlager 77 and the lower Abschfederlager 80th arranged and pushes the ejector 71 upwards, so that the upper Abschfederlager 77 abuts the overstroke spring bearing 32.
  • the lifting body 10 has a lower hollow cylindrical portion 82 and an upper hollow cylindrical portion 83, wherein the upper hollow cylindrical portion 83 has a smaller inner and outer diameter and is shorter than the lower hollow cylindrical portion 82.
  • the upper edge of the lower hollow cylindrical portion 82 is connected to the bottom edge of the upper hollow cylindrical portion 83 connected by an annular disc-shaped shoulder 84.
  • the lift body 10 has a plurality of axially extending slots 89 in the lower hollow cylindrical portion 82.
  • the slots 89 begin at a distance from the upper edge of the lower hollow cylindrical portion 82 and extend to the lower edge of the lower hollow cylindrical portion 82.
  • four slots 89 are present which are uniformly distributed over the circumference of the lower hollow cylindrical portion 82.
  • the over-stroke spring bearing 32 has the shape of a disc with an upwardly projecting edge.
  • the over-stroke spring bearing 32 is engaged with its outer edge in recesses 90 on the inside of the struts 85.
  • the recesses 90 extend for manufacturing reasons from the inside to the outside of the struts 85th
  • the lower hollow cylindrical portion 82 has an outer thread 91 at the outer periphery from the upper edge.
  • the slots 89 extend into a lower portion of the outer thread 91.
  • a circular cylindrical threaded ring 92 with an internal thread 93 is screwed onto the external thread 91.
  • the threaded ring 92 has at the top of an inwardly projecting, further annular disc-shaped shoulder 94, which is arranged above the shoulder 84.
  • On the upper edge or on the further paragraph 94 is a toothing 95 with parallel to the screw axis of the internal thread 93 facing teeth available.
  • the toothing 95 extends along a helix 96 about the helical axis of the internal thread 93, which has the same pitch as the external thread 91 and the internal thread 93.
  • the toothing 95 is a bevel gear.
  • the stopper body 27 is pressed by the overstroke spring 31 with the projections 29 against the lower edge of the threaded ring 92.
  • markings 104 are arranged in the form of Arabic numerals. Starting from a central arranged digit 0 numbers 1 2 3 ... are arranged in ascending order in the various circumferential directions. The marks 104 are evenly distributed over the circumference.
  • the markings 104 are arranged on a further helical line 105 which has the same pitch as the external thread 91 and the internal thread 93 (cf. Fig. 9 ).
  • the screw ring 92 is arranged in the upper housing part 2 below a window 106, so that in each case a marking 104 is visible from the outside. Furthermore, the drive gear 100 is arranged below a hole 107 in the housing upper part 2, so that a tool in the form of a hexagonal wrench can be inserted into the tool attachment 103 from the outside.
  • the pipette 1 can be used as follows:
  • Remains remaining in the pipette tip 51 can be blown out by pressing the actuating element 4 further down under increased force.
  • the lower stopper body 27 is displaced against the action of the overstroke spring 31 in the guide slots 30 down and on the decoupling device 44, the drive element 34 further displaced downwards, which pushes the piston 58 even deeper into the cylinder 57.
  • a further overpressure is generated which pushes out residual liquid adhering to the inner wall of the pipette tip 51.
  • the pipette 1 has a factory calibration, i. it is calibrated by the manufacturer of the pipette 1. For this purpose, the settings of the counter 23 and the threaded spindle 8 are matched. In this tuning, the screw ring 92 is set so that the numeral 0 is visible through the window 106.
  • the user can perform a user calibration by turning the drive gear 100 in one direction or the other by means of a polygonal key.
  • the threaded ring 92 is axially displaced on the external thread 91.
  • An axially directed projection 108 adjacent to the uppermost tooth of the axially directed gear 95 prevents the drive gear 100 from disengaging the gear 95 ( Fig. 9 ).
  • the screw ring 9 tables can be made available to the user, depending on the ambient conditions (pressure, temperature) and the properties of the liquid to be pipetted (density, viscosity, surface tension) specify the to be set in the window 106 markers 104.
  • the user himself can determine suitable settings by means of measurements and adjust them during user calibration.

Claims (15)

  1. Pipette avec
    - un logement en forme de tige (2, 3),
    - un siège (50) pour le maintien amovible d'une pointe de pipette (51) à l'extrémité inférieure du logement (2, 3),
    - un dispositif de refoulement comprenant une chambre de refoulement (57) avec une limite pouvant être déplacée (58),
    - un canal de connexion (56) connectant la chambre de refoulement (57) à une ouverture dans le siège,
    - un dispositif d'entraînement accouplé à la limite pouvant être déplacée (58), présentant une tige de piston (6) pouvant être déplacée axialement pour déplacer la limite pouvant être déplacée (58) de la chambre de refoulement (57),
    - un corps de butée supérieur, un corps de butée inférieur (27) et un élément de butée (25) à la périphérie de la tige de piston (6) pour limiter la course de la tige de piston (6),
    - un ressort de dépassement de course (31) par le biais duquel le corps de butée inférieur (27) est appuyé sur un palier de ressort de dépassement de course (32) à l'encontre d'un déplacement vers le bas,
    - un élément de vis fixe (10) connecté de manière fixe au logement (2, 3) et un élément de vis (92) en engagement avec celui-ci, pouvant être déplacé dans le logement, qui est accouplé au corps de butée inférieur (27) pour déplacer celui-ci dans la direction axiale de la tige de piston (6) lors d'un déplacement de l'élément de vis pouvant être déplacé (92),
    - caractérisée par
    - un engrènement (95) s'étendant le long d'une hélice (96) avec la même pente que le filetage (93) de l'élément de vis pouvant être déplacé (92) avec des dents orientées en direction de l'axe de vis de l'élément de vis pouvant être déplacé (92) sur le bord supérieur de l'élément de vis pouvant être déplacé (92),
    - une roue dentée d'entraînement (100) logée à rotation sur un palier (97) connecté de manière fixe au logement (2, 3) qui s'engrène avec l'engrènement (95) de l'élément de vis pouvant être déplacé (92) et
    - des moyens (103) pour faire tourner la roue dentée d'entraînement (100).
  2. Pipette selon la revendication 1, dans laquelle l'élément de vis pouvant être déplacé (92) est un anneau fileté disposé de manière concentrique à la tige de piston avec un filetage (93) à la périphérie qui présente l'engrènement (95) en haut et repose en bas sur le corps de butée inférieur (27).
  3. Pipette selon la revendication 2, dans laquelle l'élément de vis fixe (10) est un corps de course cylindrique creux qui présente à la périphérie externe un filetage externe (91) avec lequel un filetage interne (93) s'engage à la périphérie interne de l'anneau fileté (92), le corps de course (10) présente plusieurs fentes (89) étendues axialement, le corps de butée inférieur (27) est disposé dans le corps de course (10) et le corps de butée inférieur (27) présente plusieurs saillies (29) dépassant vers l'extérieur, lesquelles pénètrent dans les fentes (89) et ont des sections dépassant du corps de course (10) sur lesquelles repose l'anneau fileté (92).
  4. Pipette selon la revendication 3, dans laquelle le palier de ressort de dépassement de course (32) est disposé dans le corps de course (10) et le ressort de dépassement de course (31) est disposé dans le corps de course (10) entre le corps de butée inférieur (27) et le palier de ressort de dépassement de course (32).
  5. Pipette selon une des revendications 1 à 4, dans laquelle le corps de butée supérieur (8) est une broche filetée qui est vissée dans un écrou de broche (9) connecté de manière fixe au logement (2, 3) et qui présente un canal de passage supérieur (7) à travers lequel la tige de piston (6) est passée et/ou le corps de butée inférieur (27) est en forme de disque et a un canal de passage inférieur (28) à travers lequel la tige de piston (6) est étendue.
  6. Pipette selon la revendication 5, dans laquelle le corps de course (10) est connecté en haut de manière fixe à l'écrou de broche (9).
  7. Pipette selon une des revendications 3 à 6, dans laquelle le palier (97) de la roue dentée d'entraînement (100) est connecté de manière fixe au corps de course (10) au-dessus du filetage externe (91).
  8. Pipette selon une des revendications 1 à 7, dans laquelle le corps de course (10) est connecté au logement par le biais d'une connexion à encliquetage.
  9. Pipette selon une des revendications 1 à 8, dans laquelle la roue dentée d'entraînement (100) et l'élément de vis pouvant être déplacé (92) sont des roues coniques ou roues de chant.
  10. Pipette selon une des revendications 1 à 9, qui présente un dispositif d'affichage (104, 106) pour afficher la position du corps de butée inférieur (27).
  11. Pipette selon la revendication 10, dans laquelle l'anneau fileté (92) présente à la périphérie externe des repères (104) écartés les uns des autres dans la direction périphérique et le logement présente une fenêtre (106) pour lire un repère (104) disposé sous la fenêtre (106).
  12. Pipette selon la revendication 11, dans laquelle les repères (104) sont disposés à la périphérie de l'anneau fileté (92) sur une autre hélice (105) dont la pente correspond à la pente du filetage (93) de l'élément de vis pouvant être déplacé (92).
  13. Pipette selon une des revendications 1 à 12, dans laquelle la roue dentée d'entraînement (100) présente une entaille d'outil (103) pour faire tourner la roue dentée d'entraînement et le logement présente un orifice (107) à travers lequel l'entaille d'outil (103) est accessible au moyen d'un outil introduit de l'extérieur dans l'orifice (107).
  14. Pipette selon une des revendications 1 à 13, qui présente des moyens pour limiter (108) la rotation de l'élément de vis pouvant être déplacé (92) de sorte que la dent la plus haut de l'engrènement (95) ne se dégage pas de la roue dentée d'entraînement (100).
  15. Pipette selon une des revendications 1 à 14, dans laquelle l'engrènement (95) de l'élément de vis pouvant être déplacé (92) est limité à côté de la dent la plus haute par une saillie axiale (108) qui bloque la roue dentée d'entraînement (100) à l'extrémité de l'engrènement axial (95).
EP13000207.4A 2012-02-29 2013-01-15 Pipette avec système de calibration Active EP2633915B1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL13000207T PL2633915T3 (pl) 2012-02-29 2013-01-15 Pipeta z systemem kalibracji

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE201210003846 DE102012003846B4 (de) 2012-02-29 2012-02-29 Pipette

Publications (3)

Publication Number Publication Date
EP2633915A2 EP2633915A2 (fr) 2013-09-04
EP2633915A3 EP2633915A3 (fr) 2014-07-30
EP2633915B1 true EP2633915B1 (fr) 2016-09-21

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ID=47561322

Family Applications (1)

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EP13000207.4A Active EP2633915B1 (fr) 2012-02-29 2013-01-15 Pipette avec système de calibration

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EP (1) EP2633915B1 (fr)
CN (1) CN103285952B (fr)
DE (1) DE102012003846B4 (fr)
PL (1) PL2633915T3 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3915682A1 (fr) 2020-05-25 2021-12-01 Eppendorf AG Pipette à volume de dosage réglable
EP4134166A1 (fr) 2021-08-09 2023-02-15 Eppendorf SE Pipette à utiliser avec une pointe de pipette
DE102022128810B3 (de) 2022-10-31 2024-02-01 Eppendorf Se Kolbenstange, Kolben-Zylinder-System sowie Flüssigkeitsdosiervorrichtung mit einem solchen Kolben-Zylinder-System
EP4331725A1 (fr) 2022-08-30 2024-03-06 Eppendorf SE Tige de piston et procédé de fabrication d'une telle tige de piston

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DE102014114462A1 (de) * 2013-10-15 2015-04-16 Alfred Von Schuckmann Zählwerk und Handgerät mit Zählwerk
DE102014109345A1 (de) * 2014-07-04 2016-01-07 Eppendorf Ag Pipette zum Betätigen einer Spritze
DE102016121810A1 (de) * 2016-11-14 2018-05-17 Ika-Werke Gmbh & Co. Kg Handdosiervorrichtung
US10751712B2 (en) 2017-09-19 2020-08-25 Mettler-Toledo Rainan, LLC Pipette quickset volume adjustment mechanism
US10744498B2 (en) 2017-09-19 2020-08-18 Mettler-Toledo Rainin, LLC Pipette quickset volume adjustment mechanism
CN111107938B (zh) * 2017-09-19 2022-10-11 梅特勒-托利多瑞宁有限责任公司 用于移液器的快速设定式体积调节机构
EP3560596A1 (fr) * 2018-04-23 2019-10-30 Eppendorf AG Pipette à utiliser avec une pointe de pipette et une famille de pipettes comprenant plusieurs pipettes avec des volumes nominaux différents
CN111408418A (zh) * 2019-01-07 2020-07-14 苏州赛尼特格尔实验室科技有限公司 一种电子移液器及其精度控制方法
PL3680016T3 (pl) * 2019-01-08 2022-04-04 Eppendorf Ag Pipeta do używania z końcówką
DE102020002780B3 (de) 2020-05-11 2021-10-14 Brand Gmbh + Co Kg Pipette und Verfahren zum Betreiben einer Pipette
FI3932557T3 (fi) * 2020-07-02 2023-12-28 Eppendorf Se Monikanavainen pipettipää
JP7315625B2 (ja) * 2020-07-14 2023-07-26 エッペンドルフ エスイー 一体型プランジャを備えたピペット・チップとともに使用するためのピペット
DE102021117128B4 (de) 2021-07-02 2023-03-30 Brand Gmbh + Co Kg Pipettiervorrichtung
CN115215077B (zh) * 2022-07-22 2023-12-22 上海鼎珂科技有限公司 一种竖向移动装置

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3915682A1 (fr) 2020-05-25 2021-12-01 Eppendorf AG Pipette à volume de dosage réglable
EP4134166A1 (fr) 2021-08-09 2023-02-15 Eppendorf SE Pipette à utiliser avec une pointe de pipette
EP4331725A1 (fr) 2022-08-30 2024-03-06 Eppendorf SE Tige de piston et procédé de fabrication d'une telle tige de piston
DE102022128810B3 (de) 2022-10-31 2024-02-01 Eppendorf Se Kolbenstange, Kolben-Zylinder-System sowie Flüssigkeitsdosiervorrichtung mit einem solchen Kolben-Zylinder-System

Also Published As

Publication number Publication date
EP2633915A3 (fr) 2014-07-30
DE102012003846A1 (de) 2013-08-29
PL2633915T3 (pl) 2017-06-30
CN103285952A (zh) 2013-09-11
EP2633915A2 (fr) 2013-09-04
CN103285952B (zh) 2016-01-20
DE102012003846B4 (de) 2014-12-11

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