EP3778028A1 - Pipette à volume de dosage réglable - Google Patents

Pipette à volume de dosage réglable Download PDF

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Publication number
EP3778028A1
EP3778028A1 EP19191903.4A EP19191903A EP3778028A1 EP 3778028 A1 EP3778028 A1 EP 3778028A1 EP 19191903 A EP19191903 A EP 19191903A EP 3778028 A1 EP3778028 A1 EP 3778028A1
Authority
EP
European Patent Office
Prior art keywords
toothing
sleeve
housing
adjusting sleeve
driver
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP19191903.4A
Other languages
German (de)
English (en)
Other versions
EP3778028B1 (fr
Inventor
Florian Tesch
Peter Molitor
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eppendorf SE
Original Assignee
Eppendorf SE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eppendorf SE filed Critical Eppendorf SE
Priority to EP19191903.4A priority Critical patent/EP3778028B1/fr
Priority to PL19191903T priority patent/PL3778028T3/pl
Priority to JP2020136605A priority patent/JP7075452B2/ja
Priority to US16/993,484 priority patent/US11602744B2/en
Priority to CN202010825171.3A priority patent/CN112387321B/zh
Publication of EP3778028A1 publication Critical patent/EP3778028A1/fr
Application granted granted Critical
Publication of EP3778028B1 publication Critical patent/EP3778028B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/0224—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type having mechanical means to set stroke length, e.g. movable stops
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0605—Metering of fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/12—Specific details about manufacturing devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/02—Identification, exchange or storage of information
    • B01L2300/025—Displaying results or values with integrated means
    • B01L2300/026—Drum counters

Definitions

  • the invention relates to a pipette with an adjustable metering volume.
  • Pipettes are particularly used in laboratories for dispensing liquids.
  • a pipette tip is clamped with its upper end on a seat of the pipette.
  • the seat is usually a conical or cylindrical projection with respect to a housing of the pipette, onto which a pipette tip with an upper opening of its tubular body can be clamped.
  • the pipette tip can absorb and dispense liquid through a lower opening in its tubular body.
  • Air-cushion pipettes comprise a displacement device for air which is connected to the pipette tip in a communicating manner through an opening in the seat. An air cushion is displaced by means of the displacement device so that liquid is sucked into the pipette tip and expelled from it.
  • the displacement device has a displacement chamber with a displaceable displacement element.
  • the displacement device is usually a cylinder with a piston that can be displaced therein.
  • Pipette tip is removed from the seat after use and exchanged for a fresh pipette tip. In this way, contamination through the transfer of liquid can be avoided during subsequent dosing.
  • Most pipettes have an ejection device which enables pipette tips to be ejected by pressing a button without touching the pipette tips.
  • Pipette tips for single use are mostly made of plastic.
  • the piston is coupled to a drive device which is used to move the piston in the cylinder.
  • the drive device has a lifting rod which can be displaced with a stop element between an upper and a lower stop. At the beginning of the suction of air into the cylinder is located the stop element at the lower stop. At the beginning of the displacement of air from the cylinder, the stop element rests against the upper stop. The amount of liquid absorbed or discharged depends on the stroke of the lifting rod between the lower and upper stop.
  • the DE 43 35 863 C1 and U.S. 5,531,131 describe a pipette in which an actuating button protrudes from the top of the housing and is connected to the upper end of a lifting rod which is connected to the piston at the lower end.
  • the lifting rod is passed through channels of a threaded spindle and a lower stop. It has a stop element in the form of an outwardly protruding bead which limits the movement of the lifting rod between the upper stop at the bottom of the threaded spindle and the lower stop.
  • Adjusting devices for adjusting the threaded spindle have an adjusting sleeve rotatably mounted in the housing, which protrudes from the top of the housing and in which the actuating button can be axially displaced.
  • the adjusting sleeve is non-rotatably connected to the threaded spindle via axial grooves on its inner circumference and drivers projecting radially from the upper end of the threaded spindle. Go berserk the adjusting sleeve, the threaded spindle with the upper stop and thus the dosing volume can be adjusted.
  • the adjusting sleeve has a spur gear at the lower end which is positively coupled via coupling devices with two coupling spur gears on a common axis with a spur gear of a counter.
  • the axis on which the two coupling spur gears are mounted can be shifted by means of switching devices in order to release the coupling devices. This enables the pipette to be calibrated at the factory.
  • the EP 1 743 701 B1 and US 8,133,453 B2 describes a pipette of the type described above, which additionally has an adjusting device for adjusting the position of a holder holding the lower stop relative to the cylinder and a display device for displaying the position of the holder. This makes it easier for the user to change the calibration and to find the factory calibration again.
  • the WO 01/61308 A1 describes a manually adjustable pipette which, for fine adjustment of the dosing volume, has a threaded rod which is coupled to a piston rod in a rotationally fixed manner and which can be displaced in a nut which is arranged fixedly in the housing.
  • a flange element rests on the piston rod on the underside of the threaded spindle.
  • the piston rod is passed through an axially displaceable sleeve that carries the spindle nut at the bottom.
  • a locking mechanism prevents axial displacement of the sleeve in the locked position and releases it for quick adjustment when unlocked.
  • the set dosage amount is determined by means of position sensors that detect the position of the piston rod.
  • the piston unit has an operating button at the upper end, with which both the fine adjustment of the dosing volume and the uptake and delivery of liquid by means of a pipette tip are controlled. As a result, the dispensing volume can be adjusted when pipetting come.
  • the pipette is complex due to the mechanical and electronic components.
  • the US 2019/0083969 A1 and US 2019/0083970 A1 describe a quick adjustment mechanism for the dispensing volume of a pipette.
  • One embodiment has a frame for insertion into a housing of a pipette, in which a planetary gear with an input shaft and an output shaft is arranged.
  • An operating mode selector is coupled to the input of the planetary gear.
  • the mode selector is between a direct drive position in which the planetary gear is disengaged, so that the rotation of the input shaft causes a 1: 1 rotation of the output shaft of the planetary gear, and a speed-multiplying position in which the planetary gear is engaged so that a rotation of the Input shaft produces a rotation of the output shaft at a multiple speed, displaceable.
  • Another embodiment has a carrier for insertion into a housing of a pipette, on which a gear transmission with an input shaft, an output shaft and a shaft parallel to it, a spur gear on the input shaft and a spur gear meshing therewith on the parallel shaft, a spur gear on the Output shaft and a spur gear meshing with it on the parallel shaft as well as claw clutches between the two spur gears on the input shaft and the output shaft and the two spur gears on the parallel shaft.
  • the invention is based on the object of creating a pipette which enables fine adjustment of the dosing volume and quick adjustment of the dosing volume with less structural complexity and less space requirement.
  • the pipette according to the invention has, instead of an adjusting sleeve from the prior art mentioned at the beginning, which is non-rotatably connected to the threaded spindle and protrudes from the housing with the upper end for adjusting the dosing volume, a driving sleeve and a non-rotatably connected to the threaded spindle a separate adjustment sleeve, rotatably mounted in the housing, for adjusting the dosing volume.
  • the adjustment sleeve forms a drive shaft and the driver sleeve forms an output shaft of a spur gear designed as a gearbox.
  • the gearbox comprises a switching device with which different gear stages can be switched which have different gear ratios (gear ratios) between the rotational speed of the adjusting sleeve and the rotational speed of the driver sleeve.
  • the switching device has a switching element accessible from the outside of the housing.
  • the driving sleeve and with this the threaded spindle are rotated faster or slower by turning the adjusting sleeve at the same speed.
  • the rotation of the driver sleeve is recorded and displayed by the counter. This makes it possible to set a dosing volume in two different speed levels.
  • a fast speed level enables a quick, coarse adjustment of the dispensing volume and a slow speed level enables simple and exact setting of the dispensing volume at the end of the setting process.
  • the low structural complexity of the adjustment mechanism is advantageous, since the drive shaft and output shaft of the spur gear replace the adjustment sleeve previously used and are not added as additional components.
  • Another advantage is the small footprint, since the driver sleeve and the adjustment sleeve can be integrated into the adjustment mechanism. The driver sleeve takes on the threaded spindle and the adjustment sleeve can be pushed onto the driver sleeve. As a result, no additional length section has to be made available in the housing of the pipette for the adjustment mechanism.
  • the adjusting sleeve or the driver sleeve has a plurality of toothings with different ones on the outer circumference Diameters
  • the transmission shaft has a plurality of toothings with different diameters
  • the switching device is designed to connect the transmission shaft to the adjustment sleeve or the driver sleeve in different switching stages via different pairs of teeth.
  • the rotation is transmitted in different switching stages via the transmission shaft. This type of design enables gear ratios that are smaller than 1: 1.
  • the gearbox can be designed with a greater ratio between the largest and smallest gear ratio (spread) than in a gearbox in which the drive shaft and the output shaft are connected directly to one another in direct gear and no rotation is transmitted via the countershaft.
  • the gearbox is designed so that it cannot be shifted into a direct gear with a ratio of 1: 1. This saves construction effort.
  • the diameter of the toothing is understood to mean the diameter of the rolling cylinders or rolling circles, the center of which falls in the center of two intermeshing spur gears and which touch each other at the rolling point of the two spur gears.
  • the switching devices are designed to displace the adjusting sleeve and the transmission shaft or the transmission shaft and the driver sleeve relative to one another in the axial direction.
  • at least one of the components adjusting sleeve, transmission shaft and driver sleeve can be displaced in the axial direction in the housing.
  • the switching device is an additional device which is coupled to at least one of the adjusting sleeve, transmission shaft and driver sleeve components in order to move two of these components relative to one another in the axial direction relocate.
  • the adjustment sleeve or the transmission shaft or the driver sleeve is at the same time the switching device or a component of the switching device. In this embodiment, the adjustment sleeve or the transmission shaft or the driver sleeve is used at the same time as a switching device or as a component of a switching device in order to switch different switching stages of the gearbox.
  • the gearbox has a direct gear.
  • the gearbox has a gear stage in which the drive shaft is connected directly to the output shaft. In another switching stage, the rotary movement is transmitted from the drive shaft to the output shaft via the transmission shaft.
  • the gearbox is designed with more than two gear stages.
  • additional teeth can be arranged on the adjustment sleeve and on the transmission shaft.
  • the manual transmission with more than two gear stages can be a manual transmission without a direct gear or a manual transmission with a direct gear.
  • the driver sleeve has a first toothing on the outer circumference
  • the adjusting sleeve has a second toothing on the outer circumference and a third toothing above the second toothing, the second toothing having a different diameter than the third toothing
  • the transmission shaft one with the first toothing meshing fourth toothing, above a fifth toothing and above a sixth toothing, wherein the fifth toothing and the sixth toothing have different diameters
  • the switching device is designed to move the adjusting sleeve and the transmission shaft in the axial direction relative to each other, so that optionally the second toothing in engagement with the fifth toothing and at the same time the third toothing out of engagement with the sixth toothing can be brought or the third toothing can be brought into engagement with the sixth toothing and at the same time the second toothing can be brought out of engagement with the fifth toothing, whereby the translation between the speed of rotation of the adjusting sleeve and the speed of rotation of the driving sleeve can be changed.
  • the first toothing is non-rotatably connected to the driver sleeve
  • the second and third toothing are non-rotatably connected to the adjustment sleeve
  • the fourth, fifth and sixth teeth are non-rotatably connected to the transmission shaft.
  • a toothing of the adjusting sleeve is in engagement with a toothing of the transmission shaft in different switching stages. Depending on the switching stage, different pairs of teeth are brought into engagement with one another. The rotation of the transmission shaft is transmitted to the driver sleeve via additional teeth. As a result, different translations are generated between the adjustment sleeve and the driver sleeve and thus the threaded spindle and the counter.
  • the speed levels are determined by the gears with different diameters on the adjustment sleeve and the gears with different diameters on the transmission shaft.
  • the threaded spindle is rotated faster or slower depending on which pair of teeth of the adjustment sleeve and transmission shaft is in engagement.
  • the gearbox is designed so that it cannot be shifted into a direct gear with a ratio of 1: 1.
  • the fourth toothing has the same diameter and the same number of teeth as the fifth toothing.
  • the ratio between the speed of rotation of the adjusting sleeve and the speed of rotation of the driving sleeve is 1: 1 if the second toothing has the same number of teeth and the same diameter as the first toothing and the second toothing meshes with the fifth toothing.
  • the fourth toothing is also the fifth toothing.
  • the fourth toothing and the fifth toothing are combined into a single toothing.
  • the fourth toothing and the fifth toothing have the same diameter and the same number of teeth.
  • the fourth toothing meshes permanently with the first toothing and the second toothing can also be brought into engagement with the fourth toothing, which also forms the fifth toothing. So that the fourth toothing can mesh with the first toothing and the second toothing at the same time, it is formed over a sufficient length of the transmission shaft.
  • the fourth toothing and the fifth toothing are different toothings.
  • the fourth toothing and the fifth toothing can be arranged at a distance from one another or directly adjoin one another and be offset from one another in the circumferential direction.
  • the fourth toothing and the fifth toothing have different diameters.
  • the fourth toothing and the fifth toothing can be arranged at a distance from one another or directly adjacent to one another, a step being formed between the fourth toothing and the fifth toothing.
  • the ratio between the rotational speed of the adjusting sleeve and the rotational speed of the driver sleeve deviates from 1: 1 when the second toothing meshes with the fifth toothing. This makes it possible to achieve ratios below 1: 1.
  • the fourth toothing and the fifth toothing have the same diameter and the same number of teeth and the first toothing and the second toothing have different numbers of teeth and the same diameter. This results in a profile shift in the circumferential direction between the profiles of the first toothing and the second Interlocking achieved. As a result, the adjusting sleeve and the driver sleeve have different rotational speeds when the second toothing meshes with the fifth toothing.
  • the number of teeth of the first toothing and the second toothing differ by one tooth.
  • the second toothing has a larger number of teeth than the first toothing.
  • This type of embodiment is advantageous in terms of production technology compared to an embodiment type in which the fourth toothing and the fifth toothing have different diameters.
  • the counter has a drive gear which engages in a first toothing on the outer circumference of the driver sleeve.
  • the counter is driven mechanically.
  • the counter is preferably a mechanical counter, in particular a roller counter.
  • the counter is an electromechanical counter or an electronic counter.
  • the electromechanical counter or the electronic counter can also be controlled by electrical pulses that are generated by a sensor that detects the rotation of the driver sleeve.
  • the rotation of the driver sleeve can also be detected indirectly by detecting the rotation of the threaded spindle.
  • the rotation of the driver sleeve denotes its position in relation to an initial position, which can be specified in the number of full or partial rotations or in degrees.
  • the adjustment sleeve is arranged concentrically to the driver sleeve. According to a further embodiment, the adjusting sleeve is pushed onto the driver sleeve. This results in a particularly compact design.
  • the switching device is designed to move the adjusting sleeve in the axial direction relative to the housing in order to selectively engage the second toothing in engagement with the fifth toothing and at the same time the third toothing out of engagement with the sixth toothing or the third toothing in engagement with the to bring the sixth toothing and at the same time the second toothing out of engagement with the fifth toothing.
  • the switching device is a switching device which is coupled to the adjusting sleeve and constructed separately therefrom and which is designed to displace the adjusting sleeve in the axial direction.
  • the adjusting sleeve is at the same time the switching device or a component thereof.
  • a section of the adjusting sleeve protruding from the housing can be used as a switching element. By gripping the section and moving the adjusting sleeve deeper into the housing or further out of the housing, the switching element can be actuated in order to shift the gearbox into different gear stages.
  • the setting sleeve is used both for switching different switching stages and for setting the dosing volume.
  • the switching device is designed to move the transmission shaft in the axial direction relative to the housing in order to selectively engage the second toothing in engagement with the fifth toothing and at the same time the third toothing out of engagement with the sixth toothing or the third toothing in engagement with the to bring the sixth toothing and at the same time the second toothing out of engagement with the fifth toothing. In this way it can be avoided that the metering volume is unintentionally adjusted when the gearbox is switched or the gearbox is unintentionally switched when the metering volume is set.
  • the switching device is one with the transmission shaft coupled and separately formed from this switching device, which is designed to move the transmission shaft in the axial direction.
  • the transmission shaft is the switching device or a component thereof.
  • a section of the transmission shaft or a switching lever coupled to the transmission shaft protrudes outward from the housing.
  • a section of the transmission shaft or shift lever that protrudes from the housing is a shift element that can be actuated from the outside in order to shift the gearbox into different shift stages.
  • Another embodiment has a first spring device which is supported in the housing and on the lifting rod or the displacement element and which holds the lifting rod with the stop element in contact with the first stop when the operating button is relieved. This ensures that the lifting rod is automatically shifted upwards after the control button is relieved of pressure until the stop element rests against the upper stop.
  • the lifting rod is displaced by means of the control button or another control element after reaching the lower stop and possibly executing an overstroke until the stop element rests on the upper stop.
  • the pipette has a device for setting a preferred position which sets the switching device to a specific switching stage. This allows the user to assume that the pipette is set to a certain switching stage before using it.
  • the device for setting a preferred position is a second spring device.
  • the transmission shaft is arranged in a free space between the adjustment sleeve and the counter. According to a further embodiment, the transmission shaft is in a wedge-shaped space between the adjustment sleeve and the counter. This reduces the space required by the adjustment mechanism.
  • the counter has counter rollers with an axis of rotation parallel to the adjusting sleeve.
  • the counter is a roller counter.
  • the transmission shaft is arranged in a wedge-shaped free space between the counter rollers and the adjustment sleeve.
  • the adjusting element is an adjusting ring at the upper end of the adjusting sleeve.
  • the dosing volume can be adjusted by turning the setting ring, which is accessible from the outside of the housing.
  • the adjusting ring at the upper end of the adjusting sleeve can also be the switching element of the switching device.
  • control knob and the setting sleeve are connected to one another in a rotationally fixed manner and axially displaceable relative to one another via a device for rotationally fixed connection, so that the control button is at the same time the setting element for setting the setting sleeve.
  • the switching element is a slide or switch that can be actuated from the outside of the housing.
  • the displacement device comprises a displacement chamber embodied as a cylinder and a displacement element embodied as a piston which can be displaced in the cylinder.
  • top and bottom relate to terms derived therefrom such as “bottom” and “top” and “horizontal” and “vertical” "to an orientation of the pipette in which the housing with the seat is oriented vertically downwards. In this orientation, a pipette tip attached to the seat can be aimed at a vessel located below in order to suck up or dispense liquid.
  • a pipette 1 has a rod-shaped housing 2 with a lower housing part 3 and an upper housing part 4.
  • the lower housing part 3 has a tubular base body 5 with a conical bottom, from which a slender tubular, slightly conical extension 6 protrudes at the bottom has a seat 7 for attaching a pipette tip 8.
  • a displacement chamber 9 is formed in the form of a cylinder, which is connected via a connecting channel 10 to an opening 11 in the underside of the seat 7.
  • the lower housing part 3 also comprises a displacement element 12 in the form of a piston of the displacement device, which is guided into the cylinder 11 via a sealing system 13 on the top of the base.
  • the displacement element 12 has at the upper end a plate 14 which has a central dome-shaped depression on the upper side.
  • a first spring device 15 in the form of a helical spring is arranged between the plate 14 and the top of the base. The first spring device 15 presses the plate 14 from below against a closure cap 16 which is connected to the base body 5 and has a passage in the center through which the plate 14 is accessible from above.
  • the upper housing part 4 contains a lifting rod 17 which rests on the upper side of the plate 14.
  • the lower end of the lifting rod 17 engages in the recess of the plate 14.
  • an operating button 18 is fixed, which protrudes outward from the upper end of the housing 2.
  • the lifting rod 17 is passed through a central spindle bore 19 of a threaded spindle 20 which is arranged in the upper housing part 4.
  • the outside of the threaded spindle 20 has an external thread 21 which can be screwed into an internal thread 22 of a lifting body 23 which is held at the bottom on a first carrier 24 in the upper housing part 4.
  • the lifting body 23 forms a spindle nut.
  • the lower end face of the threaded spindle 20 is an upper stop 25 for a stop element 26 in the form of an annular bead on the outer circumference of the lifting rod 17.
  • the threaded spindle 20 is non-rotatably connected at the upper end to a driver 27, which by means of radially outwardly projecting ribs 28 (cf. Fig. 5 ) engages in axial grooves 29 of a driver sleeve 30.
  • the driver sleeve 30 is arranged concentrically to the threaded spindle 20 and rotatably mounted on the outer circumference of the lifting body 23.
  • the driver sleeve 30 has a circumferential first toothing 31 at the lower edge on the outer circumference. This is particularly evident in FIG Figs. 3 to 5 shown.
  • An adjusting sleeve 32 is pushed onto the driver sleeve 30.
  • the adjustment sleeve 32 is rotatably mounted on the outer circumference of the driver sleeve 30 and is guided on the driver sleeve 30 so that it can be displaced in the axial direction between two limits.
  • the upper end of the adjustment sleeve 32 projects outward from the upper end of the housing 2.
  • the adjusting sleeve 32 has an adjusting ring 33 on the outer circumference, which has a corrugation on the outer circumference.
  • the adjusting sleeve 32 has on the lower edge a circumferential second toothing 34 and a little further up a circumferential third toothing 35.
  • the first toothing 31 and the second toothing 34 have the same diameter and the same number of teeth.
  • the third toothing 35 has a larger diameter and a larger number of teeth than the second toothing 34.
  • the second toothing 34 is on the upper side and the third toothing 35 is closed on the underside by an intermediate disk 36.
  • the underside of the disk 36 forms a lower limit 37 and the upper side of the disk 36 forms an upper limit 38 for the displacement of the adjusting sleeve 32.
  • a transmission shaft 39 is rotatably mounted on the first carrier 24.
  • the transmission shaft 39 is provided at the bottom with a fourth toothing 40, above it with a fifth toothing 41 and above it with a sixth toothing 42.
  • the fourth toothing 40 and the fifth toothing 41 have the same diameter and the same number of teeth and are combined into a single toothing 43.
  • the sixth toothing 42 is arranged at a distance from the fifth toothing 41. It has a smaller diameter and a smaller number of teeth than the fifth toothing 41.
  • the transmission shaft 39 is rotatably mounted at the top in a second carrier 44 which is fixed in the upper housing part 4.
  • a counter 45 in the form of a roller counter is held between the first carrier 24 and the second carrier 44.
  • a counting roller axle 46 of the roller counter is mounted at the bottom in the first carrier 24 and at the top in the second carrier 44.
  • the second carrier 44 is supported at the top on a projection in the housing.
  • a drive gear 47 is rotatably mounted on the first carrier 24, which has two spur gears 48, 49 with different diameters on one common shaft includes.
  • the spur gear 48 with the smaller diameter meshes with the first toothing 31 of the driver sleeve 30 and the spur gear 49 with the larger diameter meshes with a drive pinion 50 on an initial roller of the roller counter.
  • the number rollers 51 of the counter 45 are visible from the outside of the housing 2 through a window 52 in the upper housing part 4, which has a transparent cover 53 (cf. Fig. 2 ).
  • a cup-shaped holder 54 is arranged below the lifting body 23.
  • the holder 54 has an external thread 55 which is screwed into an internal thread 56 of a third carrier 57 fastened in the housing 2.
  • the holder 54 contains a cap-shaped lower stop 58 which is held under an upper edge 59 of the holder 54 which is bent downwards.
  • An overtravel spring 60 in the form of a helical spring, which is supported on the bottom 61 of the holder 54, presses the lower stop 58 against the upper edge 59.
  • the lifting rod 17 is through central passages of the lower stop 58, through the overtravel spring 60 and a central passage in the Bottom 61 of the holder 54 passed through.
  • the adjustment sleeve 32 is a drive shaft
  • the driver sleeve 30 is an output shaft
  • the transmission shaft 39 is a countershaft of a gearbox 63 designed as a spur gear 62. Switching between the various gear stages is achieved by axially moving the adjustment sleeve 32 into an in Fig. 3 and 4th shown lower switching position (fine adjustment position) and into an in Fig. 4 the upper switching position shown (quick adjustment position). In the fine adjustment position of Fig.
  • the adjusting sleeve 32 is shifted downwards at most up to the contact of the lower limit 37 on the upper side of the fifth toothing 41 and in the quick adjustment position the adjusting sleeve 32 is up to the contact the upper limit 38 on the underside of the sixth toothing 42 shifted upwards.
  • the adjusting sleeve 32 is at the same time a switching device 64 of the gearbox, the adjusting ring 33 being a switching element 65 of the switching device 64.
  • the gearbox 63 does not have a preferred position, so that the gearbox always maintains the gear stage that was last set.
  • the invention encompasses other types of embodiment with a preferred position of the gearbox, which are implemented, for example, by means of a second spring device.
  • the driving sleeve 30 is rotated with it in accordance with the switching stage set.
  • the threaded spindle 20 is screwed into the internal thread 22 fixed to the housing and the upper stop 25 moves up or down depending on the direction of rotation. This adjusts the distance between the upper stop 25 and the lower stop 58, which determines the metering volume.
  • the metering volume set in each case can be read on the counter 45, which is driven by the drive sleeve 30 via the drive gear 47.
  • an ejector button 66 on an ejector rod 67 is connected to a lateral attachment attachment 68 of an ejector sleeve 69, which is arranged displaceably on attachment 6.
  • An ejector spring 70 designed as a helical spring, is arranged in the upper housing part 4, which is supported at one end in the housing 2 and at the other end engages the ejector rod 67.
  • the ejector spring 70 presses the ejector rod 67 upwards so that the ejector sleeve 67 rests against the extension 6.
  • the lower housing part 3 and the upper housing part 4 are connected to one another by a snap connection 71.
  • the user can set the desired dispensing volume before pipetting. To do this, he turns the setting ring 33 until the desired dosing volume is indicated by the counter 45.
  • the user can choose between two speed levels for setting the dosing volume. In particular, if the last set dosing volume deviates significantly from the dosing volume to be set, the user can first select a fast switching level. If the gearbox is not already set to the fast gear stage, the user grips the setting ring 33 and pulls the setting sleeve 32 out of the fine adjustment position Fig. 3 a little further out of the housing 2 into the in Fig. 4 quick adjustment position shown.
  • the first toothing 31 of the driver sleeve 30 meshes with the fourth toothing 40 of the transmission shaft 39 and meshes the third toothing 35 of the adjusting sleeve 32 with the sixth toothing 42 of the transmission shaft 39.
  • This increases the rotational speed of the adjusting sleeve 32 to a higher rotational speed of the driving sleeve 30 translated so that the user can quickly set the dosing volume close to the dosing volume to be set.
  • the user can select a slow switching level for the precise setting of the desired dispensing volume. To do this, he presses the setting sleeve 32 deeper into the housing 2 on the setting ring 33, up to the in Fig. 3 Fine adjustment position shown. In this position, the first toothing 31 meshes with the fourth toothing 40 and the second toothing 34 with the fifth toothing 41. As a result, a rotation of the adjusting sleeve 32 at a certain rotational speed results in a rotation of the driver sleeve 30 at a lower rate Speed than in the fast switching stage.
  • the speed of rotation of the adjusting sleeve 32 is equal to the speed of rotation of the driving sleeve 30, since the first toothing 31 and the second toothing 34 and the fourth toothing 40 and the fifth toothing 41 each have the same number of teeth and diameter.
  • the user can clamp a pipette tip 8 onto the pipette 1 by pressing the pipette 1 with the seat 7 into the upper opening 72 of the pipette tip 8.
  • he first presses the control button 18 downwards so that the stop element 26 is displaced from the upper stop 25 against the lower stop 58.
  • the lifting rod 17 presses the displacement element 12 downwards and the first spring device 15 is pretensioned.
  • the user dips the pipette tip 8 with its lower opening 73 into the sample liquid and relieves the control button 18.
  • the first spring device 15 pushes the displacement element 12 and the lifting rod 17 upwards until the stop element 26 rests against the upper stop 25.
  • a quantity of liquid corresponding to the set metering volume is sucked into the pipette tip 8.
  • the user holds the pipette tip 8 with the lower opening 73 over another vessel and presses the control button 18 downwards again. After reaching the lower stop 58, he can press the operating button 18 even deeper, overcoming the resistance of the overtravel spring 60, in order to eject a residual amount of liquid from the pipette tip 8.
  • a further quantity of liquid can then be pipetted in the same way or, to change the sample liquid, the pipette tip 8 can be ejected by pressing the ejector button 66 downwards.
  • the ejector sleeve 69 strips the pipette tip 8 from the seat 7.
  • the Ejector button 66 moves the ejector spring 70 the ejector rod 67 back into the starting position shown.
  • the Fig. 6 shows the first and second toothing 31 ', 34' of a further embodiment. This differs from the previously described ones in that the first toothing 31 'and the second toothing 34' have the same diameter, although the first toothing 31 'has one tooth less than the second toothing 34'. As a result, the first toothing 31 'has a larger pitch than the second toothing 34'.
  • the top view shows that, as a result, there is a profile shift of the teeth between the first toothing 31 'and the second toothing 34'.
  • the fourth toothing 40 and the fifth toothing 41 each have the same diameter and the same number of teeth.
  • the driver sleeve 30 performs a little less than one revolution.
  • the speed of rotation of the adjusting sleeve 32 is greater than the speed of rotation of the driving sleeve 30.
  • the driving sleeve 30 is rotated as in the above embodiment. Overall, a somewhat larger spread of the ratios is achieved.
  • the adjustment mechanism of Fig. 7 and 8th differs from the adjustment mechanism of Fig. 3 and 4th in that it is not the adjusting sleeve 32 but rather the transmission shaft 39 which is axially displaceable in the housing 2 and which is a component of the switching device 64. Accordingly, two prevent Limits the displacement of the adjusting sleeve 32 in the axial direction.
  • the displacement of the transmission shaft 39 in the axial direction is made possible by the fact that it engages with its two ends in bearings in the first carrier 24 and in the second carrier 44, which allow a corresponding displacement.
  • a shift lever 74 is seated on the transmission shaft 39 and protrudes perpendicularly from the transmission shaft 39 to the outside.
  • the switching lever 74 is the switching element 65 of the switching device 64.
  • the switching lever 74 is held in a groove on the circumference of the transmission shaft 39.
  • the switching lever 74 is snapped onto the transmission shaft 39 with a fork-shaped end, for example.
  • the shift lever 74 engages in the annular groove in such a way that, by moving the shift lever 74 in the axial direction, the transmission shaft can be shifted up and down and the transmission shaft 39 can be rotated with respect to the shift lever 74.
  • the switching lever 74 With one outer end, the switching lever 74 consists of a vertical slot in the housing 2, so that it can be displaced from the outside.
  • the transfer shaft 39 is shifted downwards (cf. Fig. 8 ) and when the shift lever 74 rests on the second carrier 44, the upward displacement of the transmission shaft 39 is limited (cf. Fig. 7 ).
  • the switching device 64 is switched to the fine adjustment position in which a rotation of the adjusting sleeve 32 causes a slow adjustment of the threaded spindle 20.
  • the switching device 64 is switched to the quick adjustment position, in which a turning of the adjusting sleeve 32 effects a quick adjustment of the threaded spindle 20.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Transmission Devices (AREA)
  • Structure Of Transmissions (AREA)
EP19191903.4A 2019-08-15 2019-08-15 Pipette à volume de dosage réglable Active EP3778028B1 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP19191903.4A EP3778028B1 (fr) 2019-08-15 2019-08-15 Pipette à volume de dosage réglable
PL19191903T PL3778028T3 (pl) 2019-08-15 2019-08-15 Pipeta o nastawnej pojemności dozowania
JP2020136605A JP7075452B2 (ja) 2019-08-15 2020-08-13 容量が調節可能なピペット
US16/993,484 US11602744B2 (en) 2019-08-15 2020-08-14 Pipette with adjustable dosing volume
CN202010825171.3A CN112387321B (zh) 2019-08-15 2020-08-17 具有可调的计量体积的移液器

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP19191903.4A EP3778028B1 (fr) 2019-08-15 2019-08-15 Pipette à volume de dosage réglable

Publications (2)

Publication Number Publication Date
EP3778028A1 true EP3778028A1 (fr) 2021-02-17
EP3778028B1 EP3778028B1 (fr) 2021-10-06

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
EP19191903.4A Active EP3778028B1 (fr) 2019-08-15 2019-08-15 Pipette à volume de dosage réglable

Country Status (5)

Country Link
US (1) US11602744B2 (fr)
EP (1) EP3778028B1 (fr)
JP (1) JP7075452B2 (fr)
CN (1) CN112387321B (fr)
PL (1) PL3778028T3 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11458466B2 (en) * 2019-05-14 2022-10-04 Gilson S.A.S. Pipette system with interchangeable volume counter
CN114505108B (zh) * 2022-03-01 2023-05-12 上海科进生物技术有限公司 一种移液装置及调节方法

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4335863C1 (de) 1993-10-21 1995-02-02 Eppendorf Geraetebau Netheler Kolbenhubpipette
WO2001061308A1 (fr) 2000-02-17 2001-08-23 Rainin Instrument Llc. Pipette manuelle a volume ajustable a reglage automatique du volume
EP1743701A1 (fr) 2005-07-16 2007-01-17 Eppendorf Ag Pipette à piston
EP2165765A1 (fr) * 2008-09-12 2010-03-24 Eppendorf Ag Dispositif de pipetage avec filetage double
EP2329885A1 (fr) 2009-12-07 2011-06-08 Fukae Kasei Co., Ltd. Dispositif de pipette
US20190083969A1 (en) 2017-09-19 2019-03-21 Mettler-Toledo Rainin, LLC Pipette quickset volume adjustment mechanism
US20190083970A1 (en) 2017-09-19 2019-03-21 Mettler-Toledo Rainin, LLC Pipette quickset volume adjustment mechanism

Family Cites Families (8)

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Publication number Priority date Publication date Assignee Title
GB1597336A (en) 1977-06-15 1981-09-03 Sherwood Medical Ind Inc Micropipettors
US4096751A (en) * 1977-06-15 1978-06-27 Oxford Laboratories Inc. Hand-held micropipettor with fluid transfer volume adjustment mechanism
EP0181957A1 (fr) 1984-11-20 1986-05-28 HTL High-Tech Lab Herstellung und Vertrieb medizinisch technischer Erzeugnisse GmbH Pipette à dose réglable
US7175813B2 (en) 2003-12-31 2007-02-13 Rainin Instrument, Llc Volume adjustable manual pipette with quick set volume adjustment
PL220934B1 (pl) * 2009-08-28 2016-01-29 PZ HTL Spółka Akcyjna Pipeta mechaniczna z nastawianą wartością objętości pobieranej cieczy
FR3010518A1 (fr) 2013-09-12 2015-03-13 Gilson Sas Pipette de prelevement presentant un dispositif ameliore de reglage et d'affichage d'un volume a prelever
JP7228576B2 (ja) * 2017-09-19 2023-02-24 メトラー－トレド・ライニン，エルエルシー ピペットのクイックセットボリューム調整機構
FR3080050B1 (fr) * 2018-04-17 2020-03-27 Gilson Sas Dispositif de verrouillage d'une vis de reglage de volume pour un systeme de pipetage

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4335863C1 (de) 1993-10-21 1995-02-02 Eppendorf Geraetebau Netheler Kolbenhubpipette
US5531131A (en) 1993-10-21 1996-07-02 Eppendorf-Netheler-Hinz Gmbh Device for adjusting correction factor of a plunger lift pipet
WO2001061308A1 (fr) 2000-02-17 2001-08-23 Rainin Instrument Llc. Pipette manuelle a volume ajustable a reglage automatique du volume
EP1743701A1 (fr) 2005-07-16 2007-01-17 Eppendorf Ag Pipette à piston
US8133453B2 (en) 2005-07-16 2012-03-13 Eppendorf Ag Plunger stroke pipette
EP2165765A1 (fr) * 2008-09-12 2010-03-24 Eppendorf Ag Dispositif de pipetage avec filetage double
EP2329885A1 (fr) 2009-12-07 2011-06-08 Fukae Kasei Co., Ltd. Dispositif de pipette
US20190083969A1 (en) 2017-09-19 2019-03-21 Mettler-Toledo Rainin, LLC Pipette quickset volume adjustment mechanism
US20190083970A1 (en) 2017-09-19 2019-03-21 Mettler-Toledo Rainin, LLC Pipette quickset volume adjustment mechanism

Also Published As

Publication number Publication date
PL3778028T3 (pl) 2022-03-07
EP3778028B1 (fr) 2021-10-06
US11602744B2 (en) 2023-03-14
CN112387321A (zh) 2021-02-23
CN112387321B (zh) 2022-05-03
JP7075452B2 (ja) 2022-05-25
US20210046471A1 (en) 2021-02-18
JP2021030227A (ja) 2021-03-01

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