EP3680017B1 - Pipette destinée à une utilisation avec une pointe de pipette - Google Patents

Pipette destinée à une utilisation avec une pointe de pipette Download PDF

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Publication number
EP3680017B1
EP3680017B1 EP19150847.2A EP19150847A EP3680017B1 EP 3680017 B1 EP3680017 B1 EP 3680017B1 EP 19150847 A EP19150847 A EP 19150847A EP 3680017 B1 EP3680017 B1 EP 3680017B1
Authority
EP
European Patent Office
Prior art keywords
pipette
pin
pipette tip
locking sleeve
tip
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
EP19150847.2A
Other languages
German (de)
English (en)
Other versions
EP3680017A1 (fr
Inventor
Martin Seippel
Matthias KUNSCH
Burkhardt REISCHMUTH
Tobias David
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eppendorf SE
Original Assignee
Eppendorf SE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eppendorf SE filed Critical Eppendorf SE
Priority to PL19150847T priority Critical patent/PL3680017T3/pl
Priority to EP19150847.2A priority patent/EP3680017B1/fr
Priority to US16/735,066 priority patent/US11554367B2/en
Priority to JP2020001047A priority patent/JP7483379B2/ja
Priority to CN202010017287.4A priority patent/CN111408422B/zh
Publication of EP3680017A1 publication Critical patent/EP3680017A1/fr
Application granted granted Critical
Publication of EP3680017B1 publication Critical patent/EP3680017B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02Burettes; Pipettes
    • B01L3/0275Interchangeable or disposable dispensing tips
    • B01L3/0279Interchangeable or disposable dispensing tips co-operating with positive ejection means
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02Burettes; Pipettes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02Burettes; Pipettes
    • B01L3/021Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/0227Details of motor drive means
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00Supporting devices; Holding devices
    • B01L9/54Supports specially adapted for pipettes and burettes
    • B01L9/547Supports specially adapted for pipettes and burettes for dispensing pins
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/06Auxiliary integrated devices, integrated components
    • B01L2300/0609Holders integrated in container to position an object
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0832Geometry, shape and general structure cylindrical, tube shaped
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0475Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0478Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure pistons
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02Burettes; Pipettes
    • B01L3/021Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02Burettes; Pipettes
    • B01L3/0286Ergonomic aspects, e.g. form or arrangement of controls

Definitions

  • the invention relates to a pipette for use with a pipette tip.
  • the liquids can in particular be homogeneous (single-phase) liquids, consisting of a single liquid component or a homogeneous mixture of several liquid components (solutions). Furthermore, the liquids can be heterogeneous (multiphase) mixtures of a liquid with another liquid (emulsions) or a solid (suspensions).
  • Pipettes have a stem-shaped pipette housing with a pin (shoulder) at the lower end for clamping on a pipette tip.
  • the pin is often a conical, cylindrical or partially conical and cylindrical projection and is also referred to as a "working cone”.
  • a pipette tip is a hollow tube with a tip opening at the lower end and an attachment opening at the upper end, with which the pipette tip can be clamped onto the pin.
  • the liquid is taken up in the pipette tip and dispensed from it. The uptake and delivery of the liquid is controlled by means of the pipette.
  • Fixed volume pipettes are used to pipette constant volumes. With variable pipettes, the volume to be dispensed can be set.
  • a mechanical counter is used to display the set volume.
  • the stroke of a drive device can be adjusted by means of an adjusting device which is coupled to the counter.
  • the pipette tip is detached from the base after use and can be exchanged for a fresh pipette tip. In this way, cross-contamination can be avoided during subsequent pipetting operations.
  • Air-cushion pipettes have a piston-cylinder system in the pipette housing, which is connected to a through hole in the pin via a channel. Pipette tips for air-cushion pipettes (air-cushion pipette tips) do not have an integrated piston.
  • Positive displacement pipettes are used with pipette tips with an integrated piston (positive displacement pipette tips).
  • This type of pipette has a pin for fastening the pipette tip and a drive device, which can be coupled to the integrated piston (tip piston), for moving the piston.
  • the piston comes in direct contact with the liquid, eliminating the effects of an air cushion.
  • Positive displacement pipettes are particularly suitable for dosing liquids with high vapor pressure, high viscosity or high density and applications in molecular biology where freedom from aerosols is important in order to avoid contamination.
  • Air-cushion or positive displacement pipette tips for single use or for re-use are made of plastic or glass.
  • the drive device has a lifting rod for moving a piston in a pipette tip, which has a hollow lower lifting rod part and an upper lifting rod part inserted into the lower lifting rod part from above.
  • the upper lifting rod part is connected to an operating element which protrudes from the upper end of the pipette housing.
  • a Mastertip® pipette tip from Eppendorf AG is can be clamped onto a pin of the pipette. By pressing the operating element, the lifting rod can be moved downwards so that an upper end of the piston rod of a tip piston of the pipette tip is pressed into the lower lifting rod part.
  • a spring device is pretensioned.
  • the spring device moves the lifting rod up to an upper stop, the tip piston being taken along and liquid being sucked into the pipette tip.
  • the sucked-in liquid can be dispensed by pressing the control element again as far as the lower stop.
  • the user To release the pipette tip, the user must press the control element with increased force so that another spring device compresses, the upper lifting rod part is shifted downwards in the lower lifting rod part, the piston is pushed out of the lower lifting rod part and the pipette tip is pushed off the pin.
  • the spring action of the spring devices must be overcome. This can be tiring for the user, especially if the pipette tips have to be replaced frequently. Furthermore, when dispensing highly viscous liquids and when dispensing liquids quickly from the pipette tip, the pipette tip may become detached from the pin due to the increased flow resistance in the tip opening.
  • the EP 0 992 288 B1 describes a pipette with an ejection device for a pipette tip into which a traction mechanism, pressure mechanism or articulated mechanism is integrated.
  • the ejection rod acting on the pipette tip covers a shorter distance than the drive rod connected to an ejection button, so that the ejection force is greater than the force for actuating the ejection device.
  • the ejection device is in the pipette housing on the side next to the drive device for the displacement device arranged, whereby the pipette has a large volume.
  • the pipette has the eject button in addition to the control button, so that the user has to change his grip to eject the pipette tip after pipetting.
  • the DE 103 55 914 B3 describes a pipette with an ejection device which converts an axial movement of the actuating button into an axial and rotary movement of the ejector with respect to the attachment. This is intended to reduce the effort required to operate the ejector.
  • the EP 1 689 528 B1 describes a pipette with a tip removal mechanism that includes a ramp member that is rotatable, has a ramp surface forming a circle, and includes one or more segments, the ramp surface having a high point and a low point in each segment.
  • a release element of the release device is to be forced to first move towards the tip and then to return.
  • the known ejection device is also arranged laterally next to the drive device, whereby the structural volume of the pipette is large.
  • the ejection device has a separate operating button. This is designed as an electrical button that controls a motor that rotates the ramp element via a gear. The structural effort of the discharge device is great.
  • the DE 27 11 124 C2 describes a pipette that can be connected to a pipette tip via a bayonet lock.
  • the pipette tip must be removed from the pipette by hand. This can lead to contamination.
  • the DE 10 2006 036 764 B4 describes a pipette tip which has a latching element for latching with a slip-on shaft and a sealing area for sealing on the slip-on shaft.
  • the pipette tip has a slot running through at least one slot in the axial direction of the pipette tip weakened wall. In this way, the sealing fixing of the pipette tip on the slip-on shaft and the detachment of the pipette tip from the slip-on shaft can be facilitated.
  • the pipette tip can be pushed off the shaft.
  • the invention is based on the object of providing a pipette in which the forces for connecting a pipette tip to the peg and for releasing the pipette tip from the peg are reduced and the pipette tip is still held sufficiently firmly on the peg so that it is is not detached from the spigot when pipetting highly viscous liquids and when pipetting quickly.
  • the first means for positive connection and the second means for positive connection are designed to be complementary to one another, so that they enter into a positive connection with one another when the pipette tip assumes a certain position on the pin.
  • the connection of the pipette tip to the peg is facilitated by the fact that the peg is elastically narrowed by pushing on the pipette tip and / or the pipette tip can be elastically widened by the penetration of the peg.
  • the elastic narrowing and / or widening is brought about by the forces acting between the first and second means for form-fitting connection when the pipette tip is pushed onto the pin.
  • the elastic narrowing and / or elastic widening is completely or partially reversed if, when sliding on, the second means for form-fitting connection of the pipette tip enter into the form-fitting connection with the first means for form-fitting connection of the pin.
  • the at least one locking sleeve is then moved into the locking position. This secures the pipette tip on the peg, since the locking sleeve in the locked position prevents the peg from being elastically narrowed and / or the pipette tip being elastically widened, which is necessary to break the positive connection between the peg and the pipette tip.
  • the locking sleeve is removed from the locking position, so that it at least partially releases the peg and / or the pipette tip and the peg is elastically narrowed and / or the pipette tip can be elastically expanded.
  • the positive connection can be broken with little effort and the pipette tip can be detached from the pin.
  • the pin has at least one longitudinally extending slot and in the blocking position the locking sleeve delimits the pin on the inside and / or the locking sleeve is formed with at least one longitudinally extending slot at the upper end and positively connected to the pin To limit the pipette tip in the locked position outside.
  • the pin for the elastic constriction has at least one slot running in the longitudinal direction.
  • the pin can consist of a hard-elastic or soft-elastic material, for example a metal or a plastic.
  • a pin made from a soft elastic material, for example from silicone rubber, a thermoplastic elastomer or rubber, can have sufficient elasticity for the elastic constriction even without a slot.
  • the pipette tip has at least one slot running in the longitudinal direction for the elastic expansion at the upper end.
  • the pipette tip can consist of a hard-elastic plastic, for example polypropylene or polyethylene, or a soft-elastic plastic. It is also possible to make a pipette tip at least at the upper end from a soft elastic material, for example from silicone rubber, a thermoplastic elastomer or from rubber, so that it has sufficient elasticity for elastic expansion even without a slot.
  • the pipette tip can be manufactured from several materials by multi-component injection molding. The different materials can be connected to one another in a form-fitting and / or chemical manner.
  • the locking sleeve is designed in such a way that it does not deform when subjected to a radial force which is suitable for elastically narrowing the pin and / or elastically expanding the pipette tip.
  • the locking sleeve consists e.g. of a metal or a hard-elastic or rigid plastic.
  • the locking sleeve is connected to a first operating element protruding from the pipette housing and displaceable relative to the pipette housing, in order to activate the first operating element to move the locking sleeve to shift down into the locked position and up from the locked position.
  • the first operating element simplifies the shifting of the locking element into the locking position and upwards out of the locking position for the user.
  • the locking sleeve is not connected to a first operating element, but can be moved by hand.
  • the pipette has an ejection device which comprises a ejection rod which is displaceably guided in the housing in the longitudinal direction of the pin and comprises a second operating element connected to the ejection rod in order to displace the ejection rod, the ejection device being designed by displacement of the ejector rod to push a pipette tip held on the pin down from the pin when the locking sleeve at least partially releases the pin and / or the pipette tip.
  • This makes it easier for the user to throw off the pipette tip and avoids contamination, in particular because the user does not have to touch the pipette tip in order to detach it from the plug.
  • the user can detach the pipette tip from the pin by hand.
  • the pipette is a positive displacement pipette or an air-cushion pipette.
  • the ejection rod When designed as a positive displacement pipette, the ejection rod is arranged within an axial bore in a lifting rod for displacing a tip piston in the pipette tip and with the lower end above a receptacle of the lifting rod for the lower end of the tip piston on the pin and (ii.) when designed as an air-cushion pipette in the pipette housing, a displacement device with a displacement device which can be displaced by a drive element of the drive device Displacement element is present, which is connected to a through hole in the pin, and the lower end of the ejection rod is arranged laterally next to the pin.
  • a pipette tip including a syringe plunger is ejected from a positive displacement pipette
  • a pipette tip is ejected from an air-cushion pipette
  • the locking sleeve and the ejector rod are connected to a transmission device which comprises the first operating element and the second operating element and is designed to control the displacement of the locking sleeve and the ejector rod in the opposite direction when at least one operating element is actuated, so that one on the Pin held pipette tip is prevented from detaching from the pin by moving the ejector rod upwards and the locking sleeve downwards and the pipette tip can be detached from the pin by moving the locking sleeve upwards and the ejector rod downwards.
  • the gear mechanism enables synchronized displacement of the locking sleeve and the ejection rod in opposite directions in order to either hold the pipette tip securely on the pipette or to release the secure hold of the pipette tip and detach the pipette tip from the pipette. This makes it easier for the user to use the pipette tip.
  • the gear device is designed to first move the locking sleeve upwards when a pipette tip is pressed and then to push the pipette tip off the attachment by moving the ejector rod downwards. In this way it can be achieved that the pipette tip is pressed off the pin only after the secure hold of the pipette tip on the pin has been released.
  • the ejector rod is already pressing against the pipette tip when the locking sleeve is still in the locking position.
  • the same operating element controls the displacement of the locking sleeve and the displacement of the ejector rod.
  • the first control element is also the second control element.
  • the same operating element controls the drive device.
  • the operating element drives the drive device.
  • the pipette has an operating element for actuating the locking sleeve and the ejection device and an additional operating element, which is different from this operating element, for controlling the drive device.
  • the transmission device is a gear transmission which has two outputs with a first and a second output-side rack, which are connected to the locking sleeve and the ejector rod and which has a first drive with a first drive-side rack, which is connected to a first actuating element
  • the first drive-side rack is connected to the second output-side rack via a drive pinion and a first output pinion rigidly connected to the drive pinion and connected to the first output-side rack via the drive pinion, an intermediate gear and a second output pinion rigidly connected to the intermediate gear.
  • the transmission device has a second drive with a second drive-side rack, which has a second control element at the end remote from the first control element, the two drive-side racks meshing with the same drive pinion on different sides in order to selectively operate one of the both controls to control the movements of the locking sleeve and the ejector rod on different sides of the pipette housing.
  • the transmission device comprises a cam support rotatably mounted in the pipette housing, a first scanning element guided on a first curve on the circumference of the cam support, which protrudes from a release rod which is displaceably guided in the pipette housing in the longitudinal direction of the spigot, and a second curve on the circumference second scanning element guided by the cam support, which protrudes from a control rod which protrudes upward from the locking sleeve and together with the locking sleeve forms a locking device displaceably guided in the direction of the pin pipette housing, and an operating element protruding from the pipette housing and rotatable relative to the pipette housing.
  • the cam support is designed so that when the operating element is arranged in an initial position, the locking sleeve in a locking position limits the inside of the pin and / or limits the pipette tip on the outside, whereby the locking sleeve prevents a pipette tip connected to the pin from being detached from the pin, and by turning of the operating element, the locking sleeve can be displaced upwards, so that the pin and / or the pipette tip is at least partially released and the ejector rod moves the pipette tip away from the pin.
  • the gear mechanism with a cam carrier can be designed as in the parallel European patent application from the present day by the same applicant with the title "Pipette for use with a pipette tip" with the application number EP 19 150 808.4 described.
  • the drive device has a transmission mechanism which is designed for successive displacements of the Operating element downward, between which the operating element is shifted upward, to shift a drive element of the drive device alternately downward and upward.
  • This type of embodiment is advantageous in the case of an operating element which can be displaced in the longitudinal direction of the pin in order to drive the drive device.
  • the transmission mechanism is at least partially arranged within a cam carrier designed as a rotary sleeve. This enables space-saving accommodation.
  • the pipette housing and the transmission device have a magnet arrangement and / or a spring device which is designed to automatically move the operating element into the starting position.
  • the magnet arrangement comprises, for example, two permanent magnets or a permanent magnet and a ferromagnetic component.
  • the permanent magnets or the ferromagnetic component are held on the pipette housing and on the gear unit in such a way that they automatically move the operating element into a starting position when they come closer to one another. This can also be achieved by a spring device which is pretensioned when the gear device is displaced out of the starting position and strives to move the gear device back into the starting position.
  • a scanning element is provided concentrically to the pin, which is supported by a spring on the pipette housing and can be pretensioned by pushing a pipette tip onto the pin, so that when Detaching a pipette tip from the pin relaxes the spring and the sensing element assists in pushing the pipette tip off the pin.
  • the scanning element can in particular be a scanning element of a scanning device for scanning the collar of a pipette tip, as in FIG EP 18 168 763.3 described.
  • the pipette is a single-channel pipette or a multi-channel pipette.
  • the cam carrier can control the ejector rod of a multi-channel ejector.
  • the terms “above” and “below” as well as “vertical” and “horizontal” and terms derived therefrom, such as “above” and “below”, “upright” and “upside down” and “ one above the other” refer to an arrangement of the pipette in which the pin is oriented vertically and is located at the end of the pipette housing pointing downwards.
  • the pipette tip these details relate to a vertical alignment of the central axis of the pipette tip, the tip opening being arranged at the bottom and the plug-on opening being arranged at the top.
  • a pipette 1 designed as a positive displacement pipette has a rod-shaped (for example cylindrical) pipette housing 2.
  • a hollow cylindrical shaft 3 projects downward from the lower end of the pipette housing 2.
  • a pin 4 projects downward from the lower end of the shaft 3, which according to FIG Fig. 1 and 4th has a through hole 5 with a through hole at the lower end.
  • the inside diameter of the through hole 5 is smaller than the inside diameter of the shaft 3.
  • the pin 4 has an upper pin section 6 with the shape of a hollow cylinder and below it a lower pin section 7 with the shape of a hollow cone.
  • An annular groove 8 runs around on the outer circumference of the pin 4 between the upper pin section 6 and the lower pin section 7.
  • the upper pin section 6, the annular groove 8 and the lower pin section 7 form the first means for the positive connection 9 of the pipette with a pipette tip.
  • the pin 4 has two slots 10, 11 running in its longitudinal direction, which are diametrically opposite one another.
  • the slots 10, 11 extend from the lower end over the entire length of the pin 4.
  • a drive device 12 is present in the pipette housing 2, which comprises a transmission element 13 in the form of a transmission rod 14, a transmission mechanism 15 and a drive element 16 in the form of a lifting rod 17. Furthermore, the drive device 12 comprises an operating element 18 in the form of an operating lever 19, which is firmly connected to a carrier plate 21 via a web 20.
  • the carrier plate 21 has an oval shape with a wide rounded end and a narrow rounded end, the operating lever 19 protruding from the edge of the narrow rounded end.
  • the carrier plate 21 has a first curved slot 22 which runs approximately parallel to the contour of the narrow rounded end.
  • the carrier plate 21 has a rectangular first edge recess 23 in the middle of the first curved slot 22 on the side of the narrow rounded end.
  • the lifting rod 17 is guided into the shaft 3 and the pin 4 from above.
  • it is hollow and provided with a longitudinal slot 24 starting from the lower end.
  • the lifting rod 17 has a C-shaped cross section. Its lower end forms a receptacle 25 for the upper end of a piston rod.
  • the transmission mechanism 15 is designed such that when the operating lever 19 is displaced downward one after the other, between which the operating lever 19 is displaced upward, the lifting rod 17 is alternately displaced downward and upward. As a result, the lifting rod 17 can be shifted from an upper position to a lower position by pressing the operating lever 19 downward, the lifting rod 17 maintains the lower position during the subsequent upward displacement of the operating lever 19 and the lifting rod 17 is activated by a subsequent pressing of the Operating lever 19 shifted downwards again upwards. This can be repeated as often as you like.
  • the pipette 1 is provided with an ejection device 26.
  • This comprises a cam carrier 27 which is rotatably mounted in the pipette housing 2 and is designed as a hollow cylindrical rotating sleeve 28.
  • the rotating sleeve 28 is rotatably mounted, for example, with its outer circumference on the inner circumference of the pipette housing 2 and supported at the upper and lower end between shoulders of steps on the inner circumference of the pipette housing 2, so that it cannot be displaced in the axial direction in the pipette housing 2.
  • the axis of rotation of the rotary sleeve 28 coincides with the longitudinal axis of the pipette housing 2 and the longitudinal axis of the pin 4.
  • the rotating sleeve 28 has on two diametrically opposite sides to its axis of rotation parallel recesses 29, 30 which extend from the upper edge of the rotating sleeve 28 and end at a distance from its lower edge.
  • the rotary sleeve 28 thus consists of an annular base 31 and above it consists of two sectors 32, 33 of a circular ring that are diametrically opposite one another and which laterally delimit the two recesses 29, 30.
  • a first curve 34 and a second curve 35 are arranged on the outer circumference of the circular base 31 of the rotary sleeve 28.
  • the first curve 34 is designed as a first groove 36 in the form of an inverted (upside-down) Y.
  • the vertical part 37 of the Y extends far up on a sector 32 to just before the upper edge of the sector 32.
  • the second curve 35 is a second groove 38 on the outer circumference of the base 31 of the rotating sleeve 28 with the shape of an upright standing V.
  • the first curve 34 and the second curve 35 are arranged offset from one another by 90 ° on the circumference of the rotary sleeve.
  • the first curve 34 and the second curve 35 each extend over an angular range of less than 90 ° over the circumference of the rotary sleeve 28.
  • the ejection device 26 comprises a ejection rod 39 which comprises a strip-shaped upper ejection rod part 40 and a cylindrical lower ejection rod part 41.
  • the upper and lower ejection rod parts 40, 41 are arranged parallel to one another and laterally offset from one another.
  • the lower end of the upper ejecting rod part 40 is connected to the upper end of the lower ejecting rod part 41 via a strip-shaped connecting rod part 42 inclined at an angle to the two ejecting rod parts.
  • a first scanning element 43 in the form of a first guide pin 44 projects perpendicularly from the inside of the upper ejection rod part 40.
  • the ejector rod 39 is preferably made in one piece, for example from a rigid plastic.
  • the ejection rod 39 is guided with the guide pin 44 in the first groove 36, the connecting rod part 42 extends through the longitudinal slot 24 of the lifting rod 17 and extends with the lower ejection rod part 41 within the lifting rod 17 to just before the lower end of the same.
  • the pipette 1 comprises a locking device 45 which comprises a locking sleeve 46 and a strip-shaped control rod 47 parallel thereto.
  • the upper end of the locking sleeve 46 and the lower end of the control rod 47 are connected to one another via a second connecting rod part 48 which is inclined at an angle to the locking sleeve 46 and to the control rod 47.
  • a second scanning element 49 in the form of a second guide pin 49.1 protrudes from the inside of the control rod 47.
  • the second guide pin 49.1 is guided in the second groove 38.
  • the locking sleeve 46 is inserted into the shaft 3 from above and rests against the inside of the pin 4.
  • the lifting rod 17 and the ejection rod 39 are inserted into the locking sleeve 46 from above.
  • the operating element 19 is pushed with the first curved slot 22 onto the sector 32 of the rotary sleeve 28 on which the first groove 36 extends.
  • the rotary sleeve 28 is connected at the top to a support ring 50 which bridges the two sectors 32, 33 and stabilizes the rotary sleeve 28.
  • the support ring 50 has on the outer edge a downwardly protruding jacket 51 which laterally surrounds the two sectors 32, 33 at the outer edges. It also has a second curved slot 52 which receives the upper edge of the sector 33 which is not provided with a groove 36,38.
  • On the diametrically opposite side of the jacket 51 there is a downwardly opened rectangular second edge recess 52.1 which is designed to accommodate the web 20 between the operating lever 19 and the carrier plate 21.
  • the support ring 50 is connected to the rotating sleeve 28, for example by gluing.
  • the rotating sleeve 28 and the locking sleeve 46 as well as the operating element 18 are made, for example, of one or more rigid plastics and / or of metal.
  • the rotary sleeve 28, the support ring 50, the operating element 18 and / or the locking sleeve 46 are preferably each formed in one piece.
  • An operating button of the operating element 18 can also be made of an elastic or soft elastic plastic or rubber.
  • the operating lever 19 protrudes from the pipette housing 2 through a first housing slot running transversely to the longitudinal axis of the pipette housing 2 and extending over part of the circumference of the pipette housing 2, so that it can be operated from the outside.
  • the first housing slot is connected in the middle to a second housing slot running in the longitudinal direction of the pipette housing 2.
  • the operating lever 19 can be displaced downward from the support ring 50 along the second housing slot against the action of a spring device, sliding with the first curved slot 22 on the sector 32 of the rotating sleeve 28. After relief, the spring device automatically moves the operating lever 19 back upwards.
  • a sleeve-shaped third scanning element 53 is guided on the outside of the shaft 3.
  • a spring device in the form of a helical spring 54 guided on the shaft is supported on the underside of the pipette housing 2 and on the upper side of the third scanning element 53.
  • the third scanning element 53 is pressed from above against a stop element on the shaft 3 or pin 4 by the helical spring 54.
  • the pipette housing 2 On the top of the pipette housing 2 there is an adjusting knob 55 for adjusting a dosing volume.
  • the dosing volume can be set by turning the adjusting knob 55.
  • a counter 56 arranged underneath in the pipette housing 2 shows the respectively set dosing volume.
  • the setting knob 55 and / or the counter 56 is coupled to the transmission mechanism 15.
  • the transmission mechanism 15 is designed to change the stroke of the lifting rod 17, which is carried out by shifting the operating element 18 downwards, in accordance with the metering volume set in each case.
  • a pipette tip 57 is attached to the pin 4.
  • the pipette tip 57 comprises a tubular body 58 which has a tip opening 59 at the lower end, a collar 61 having a plug-on opening 60 at the upper end and at the inner circumference of the collar 61 a seat area 62 for clamping onto the pin 4.
  • the seat area 62 has a complementary contour to the pin 4, the bottom a conical lower seat section 63 for receiving the conical lower pin section 7, above a circumferential bead 64 for engaging in the annular groove 8 of the pin 4 and above a cylindrical upper seat section 65 for receiving the having cylindrical upper pin portion 6.
  • the lower seat section 63, the bead 64 and the upper seat section 65 form second means for the form-fitting connection 66 of the pipette tip 57 with the pipette 1.
  • the tubular body 58 has a cylindrical piston running area 67. Below that, the tubular body 58 has a downwardly tapering tip section 68 with the shape of a hollow truncated cone. The tip portion 68 is in Fig. 4 shown and omitted in the remaining drawings for the sake of simplicity.
  • a tip piston 69 is inserted in the tubular body 58. This comprises a piston 70 which is guided in the piston barrel region 67.
  • a piston rod 71 which has a smaller diameter than the piston 70, projects upward from the piston 70.
  • the piston rod 71 has a piston head 72 at the upper end. The piston head 72 is according to FIG Fig. 4 pressed down into the receptacle 25 of the reciprocating piston 17.
  • the pipette 1 can be used as follows: According to Fig. 1 and 8th a pipette tip 57 is held on the pipette 1 in an initial state.
  • the seat area 62 is connected to the pin 4 in a form-fitting manner, in particular by the bead 64 engaging in the annular groove 8.
  • the actuating element 18 is in the starting position at the upper end of the second housing slot, in which the first housing slot can be rotated in both directions.
  • the maximum angle of rotation is limited by the extent of the first and second grooves 36, 38 in the circumferential direction or the first housing slot, depending on which extension is smaller.
  • the locking sleeve 46 is according to Fig. 4 arranged in the lowest position, so that it prevents unintentional detachment of the pipette tip 57 from the pin 4.
  • a radial narrowing of the pin 4, which the locking sleeve 46 does not allow in this position, would namely be necessary for the release of the form-fitting connection.
  • the tip piston 69 is not yet pressed into the receptacle 25 of the lifting rod 17 with the piston head 72.
  • the pipette 1 with the lower end of the pipette tip 57 held on it is immersed in a liquid. Thereafter, the operating element 18 is pressed down again. This movement is converted by the transmission device 15 into a lifting movement of the lifting rod 17. As a result, the tip piston 69 is displaced upward.
  • the piston head 72 takes the ejector rod 39 with it, so that the first guide pin 44 slides upward in the vertical part 37 of the Y-shaped first groove 36. Meanwhile, the locking sleeve 46 maintains its position. This is in Fig. 9 shown.
  • the pipette tip 57 When the operating element 18 has carried out the set stroke, the pipette tip 57 is filled with a certain amount of liquid. Then the control element 18 relieved and shifted back upwards by the spring device until it rests on the support ring 50. In order to dispense this amount of liquid, the pipette 1 with the pipette tip 57 can be aligned with another vessel. By pressing the operating element 18 downwards again, the lifting rod 17 is shifted downwards and the amount of liquid is dispensed. Here, the first guide pin 44 slides down to the junction of the first groove 36.
  • the stroke performed by the operating element 18 when the liquid is taken up and dispensed depends on the set amount of liquid.
  • the uptake and release of liquid can be carried out several times.
  • the operating lever 18 is pivoted to the right or to the left in the starting position. This rotates the rotary sleeve 28 so that the second groove 38 displaces the second guide pin 49.1 and thus the locking sleeve 46 upwards until the locking sleeve 46 has released the pin 4 to such an extent that it can be deformed radially inward.
  • the locking sleeve 46 is preferably pulled out of the through hole 5.
  • the first guide pin 44 is displaced downward in one of the two lateral sections of the lower part of the first groove 36, so that the ejector rod 39 presses against the tip piston 69, which is supported at the bottom on the tip section 68.
  • the bead 64 exerts a radial force on the pin, so that it narrows and the positive connection between the pipette tip 57 and the pin 4 is broken.
  • the pipette tip 57 is released from the pin 4. This is in Fig. 10 shown.
  • the stripping of the pipette tip 57 from the pin 4 can also be assisted by the scanning element 53, which is pressed by the pretensioned helical spring 54 against the upper edge of the pipette tip 57.
  • a new pipette tip 57 can be connected to the pin 4.
  • the pipette 1 with the pin 4 can be inserted into the receiving opening 68 of a pipette tip 57 held ready in a carrier.
  • the scanning element 53 is displaced upwards and the helical spring 54 is pretensioned.
  • the piston head 72 presses against the underside of the ejection rod 39, so that the first guide pin 44 slides as far as the first branch point of the first groove 36.
  • the rotary sleeve 28 is rotated in the pipette housing 2 until the operating element 18 is in the starting position.
  • connection of the tip piston 69 to the lifting rod 17 and the pipetting can take place in the manner described above.
  • Fig. 1 differs from the one described above in that the locking sleeve 46 is pushed over the outer circumference of the collar 61 of the pipette tip 57 in the locking position.
  • pipette tips 57 are used which have at least one longitudinal slot extending from the upper end. The longitudinal slot enables the pipette tip 57 to be radially expanded in order to produce the form-fitting connection between the pipette tip 57 and the pin 4. The release of the positive connection is prevented when the locking sleeve 57 is in the locking position, as in FIG Fig. 11 shown.
  • the locking sleeve 46 is displaced upward by means of the rotating sleeve 28, as in the previously described embodiment, and then the pipette tip 57 is detached from the spigot 4 by pressing against the top of the tip piston 69.
  • a gear device 73 for displacing the locking sleeve 46 and the ejection rod 39 is designed as a gear mechanism 74.
  • the gear drive 74 has two output drives 75, 76 in the form of a first and a second output-side toothed rack 77, 78.
  • the first gear rack 77 on the output side is connected to the locking sleeve (46) via the control rod 47 and the second gear rack 78 on the output side is connected to the ejector rod 39.
  • the transmission device 73 has two drives 79, 80 in the form of a first and a second drive-side rack 81, 82.
  • the first drive-side rack 81 is connected at one end to a first control element 83 and the second drive-side rack 82 is connected to a second control element 84 at the end remote from the first control element 83.
  • the two drive-side racks 81, 82 mesh with the same drive pinion 85 on different sides.
  • the first and second drive-side racks 81, 82 are connected to the second output-side rack 78 and via the drive pinion 85 and a first output pinion 86 rigidly connected to the drive pinion 85 the drive pinion 85, an intermediate gear 88 and a second output pinion 87 rigidly connected to the intermediate gear are connected to the first output-side rack 77.
  • the first and the second operating element 83, 84 are arranged on different sides of the pipette housing 2.
  • the movement of the locking sleeve 46 and the ejection rod 39 can be controlled in different directions.
  • the gear mechanism 73 with the gear mechanism 74 can be arranged in the pipette housing 2 instead of the gear mechanism with the rotary sleeve 28.
  • the drive device 12 can be separated from the transmission device 73, it being possible for a separate operating element to be present for driving the drive device 12, that of the two operating elements 83, 84 of the transmission device 73 is separated.
  • This can be an operating element 18 which protrudes from the pipette housing 2 through a longitudinal slot running in the longitudinal direction of the pipette housing 2 and can be displaced in the direction of the pin 4.
  • the operating element 18 can shift a drive element 16 of the drive device 12 alternately upwards and downwards via a transmission mechanism 15 which is designed to shift a drive element 16 of the drive device 12 alternately downwards and upwards when the operating element 18 is displaced downwards one after the other.

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  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Claims (11)

  1. Pipette destinée à une utilisation avec une pointe de pipette, comportant
    • un boîtier de pipette (2) en forme de tige,
    • un téton (4) à l'extrémité inférieure du boîtier de pipette (2) pour le maintien d'une pointe de pipette (57), lequel présente des premiers moyens pour l'assemblage par complémentarité de forme (9) avec une pointe de pipette (57), de sorte qu'une pointe de pipette (57) présentant des deuxièmes moyens pour l'assemblage par complémentarité de forme (66) avec les premiers moyens d'assemblage par complémentarité de forme (9) peut être emmanchée sur le téton (4) par rétrécissement élastique du téton (4) et/ou par élargissement élastique de la pointe de pipette (57) avant son assemblage par complémentarité de forme avec le téton (4),
    • un dispositif d'entraînement (12) pour le déplacement d'un élément de refoulement (69) destiné à l'aspiration d'un échantillon liquide dans une pointe de pipette (57) maintenue sur le téton (4) et à l'éjection de l'échantillon hors de la pointe de pipette (57),
    • au moins un manchon d'arrêt (46) disposé de façon concentrique par rapport au téton (4), lequel est guidé dans le boîtier de pipette (2) de façon à pouvoir être déplacé vers le téton (4),
    • dans laquelle l'au moins un manchon d'arrêt (46) peut être déplacé vers une position d'arrêt dans laquelle il limite intérieurement un téton (4) apte à être rétréci par enfoncement d'une pointe de pipette (57) et/ou dans laquelle il limite extérieurement une pointe de pipette (57) apte à être élargie par emmanchement sur le téton (4), moyennant quoi le manchon d'arrêt (46) empêche un détachement, par rapport au téton (4), d'une pointe de pipette (57) reliée par complémentarité de forme au téton (4), et le manchon d'arrêt (46) peut être déplacé vers le haut pour sortir de la position d'arrêt, de sorte que le téton (4) et/ou la pointe de pipette (57) est au moins partiellement libéré(e) et la pointe de pipette (57) peut être détachée du téton (4).
  2. Pipette selon la revendication 1, dans laquelle le téton (4) présente au moins une fente (10, 11) s'étendant dans la direction longitudinale et le manchon d'arrêt (46) limite intérieurement le téton (4) dans la position d'arrêt et/ou dans laquelle, dans la position d'arrêt, le manchon d'arrêt (46) est conçu pour limiter extérieurement une pointe de pipette (57) reliée par complémentarité de forme au téton (4) et présentant, à l'extrémité supérieure, une fente s'étendant dans la direction longitudinale.
  3. Pipette selon la revendication 1 ou 2, dans lequel le manchon d'arrêt (46) est relié à un premier élément de commande (83) faisant saillie sur le boîtier de pipette (2) et déplaçable par rapport au boîtier de pipette (2) pour déplacer le manchon d'arrêt (46) vers le bas dans la position d'arrêt et vers le haut hors de la position d'arrêt, par actionnement du premier élément de commande (83).
  4. Pipette selon l'une des revendications 1 à 3, présentant un dispositif d'éjection (26) comportant une tige d'éjection (39), laquelle est guidée dans le boîtier de pipette (2) de façon à pouvoir se déplacer dans la direction longitudinale du téton (4) et comporte un deuxième élément de commande (84) relié à la tige d'éjection (39), pour déplacer la tige d'éjection (39), dans laquelle le dispositif d'éjection (26) est conçu pour retirer, par déplacement de la tige d'éjection (39) vers le bas, une pointe de pipette (57) maintenue sur le téton (4) par rapport au téton (4), lorsque le manchon d'arrêt (46) libère au moins partiellement le téton (4) et/ou la pointe de pipette (57).
  5. Pipette selon la revendication 4, dans laquelle (i) avec une réalisation sous la forme d'une pipette de refoulement direct, la tige d'éjection (39) est disposée à l'intérieur d'un alésage axial dans une tige de levage (17) pour le déplacement d'un piston de pointe (69) dans la pointe de pipette (57) et disposée avec l'extrémité inférieure au-dessus d'un logement (25) de la tige de levage (17) pour l'extrémité supérieure du piston de pointe (69) d'une pointe de pipette (57) maintenue sur le téton (4) et insérée avec le piston de pointe (69) dans un alésage traversant (5) du téton (4), et (ii) avec une réalisation sous la forme d'une pipette à coussin d'air, le boîtier de pipette (2) contient un dispositif de refoulement avec un élément de refoulement apte à être déplacé par un élément d'entraînement (16) du dispositif d'entraînement (12), lequel est relié à un alésage traversant (5) du téton (4), et l'extrémité inférieure de la tige d'éjection (39) est disposée latéralement à côté du téton (4).
  6. Pipette selon la revendication 4 ou 5, dans laquelle le manchon d'arrêt (46) et la tige d'éjection (39) sont reliés à un dispositif d'engrenage (73) comportant le premier élément de commande (83) et le deuxième élément de commande (84) et conçu pour piloter le déplacement du manchon d'arrêt (46) et de la tige d'éjection (39) dans des directions opposées lors de l'actionnement d'au moins un élément de commande (83, 84), de manière à empêcher une pointe de pipette (57) maintenue sur le téton (4) de se détacher du téton (4) par déplacement de la tige d'éjection (39) vers le haut et du manchon d'arrêt (46) vers le bas et à pouvoir détacher la pointe de pipette (57) du téton (4) par déplacement du manchon d'arrêt (46) vers le haut et de la tige d'éjection (39) vers le bas.
  7. Pipette selon la revendication 6, dans laquelle, lors du retrait d'une pointe de pipette (57), le dispositif d'engrenage (73) est conçu pour déplacer tout d'abord le manchon d'arrêt (46) vers le haut, puis pour retirer la pointe de pipette (57) de l'embout (4) par déplacement de la tige d'éjection (39) vers le bas.
  8. Pipette selon la revendication 6 ou 7, dans laquelle le même élément de commande (83, 84) pilote le déplacement du manchon d'arrêt et le déplacement de la tige d'éjection (39).
  9. Pipette selon l'une des revendications 1 à 8, dans laquelle le même élément de commande pilote le dispositif d'entraînement (12).
  10. Pipette selon l'une des revendications 6 à 9, dans laquelle le dispositif d'engrenage (73) est un engrenage à roues dentées (74), lequel présente deux sorties (75, 76) avec une première et une deuxième crémaillère coté sortie (77, 78) reliées à la tige d'éjection et au manchon d'arrêt et lequel présente une première entrée (79) avec une première crémaillère côté entrée (81) reliée à un premier élément de commande (83), dans laquelle la première crémaillère côté entrée (81) est reliée à la deuxième crémaillère côté sortie (78) par le biais d'un pignon menant (85) et d'un premier pignon mené (86) relié fixement au pignon menant (85), et reliée à la première crémaillère côté sortie (77) par le biais du pignon menant (85), d'une roue dentée intermédiaire (88) et d'un deuxième pignon mené (87) relié fixement à la roue dentée intermédiaire (88).
  11. Pipette selon la revendication 10, dans laquelle le dispositif d'engrenage (73) présente une deuxième entrée (80) avec une deuxième crémaillère côté entrée (82), laquelle présente un deuxième élément de commande (84) à l'extrémité éloignée du premier élément de commande (83), dans laquelle les deux crémaillères côté entrée (81, 82) engrènent avec le même pignon menant (85) sur des côtés différents, afin de piloter les mouvements du manchon d'arrêt (46) et de la tige d'éjection (39) par actionnement sélectif des différents éléments de commande (83, 84) sur des côtés différents du boîtier de pipette (2).
EP19150847.2A 2019-01-08 2019-01-08 Pipette destinée à une utilisation avec une pointe de pipette Active EP3680017B1 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
PL19150847T PL3680017T3 (pl) 2019-01-08 2019-01-08 Pipeta do używania z końcówką
EP19150847.2A EP3680017B1 (fr) 2019-01-08 2019-01-08 Pipette destinée à une utilisation avec une pointe de pipette
US16/735,066 US11554367B2 (en) 2019-01-08 2020-01-06 Pipette for use with a pipette tip
JP2020001047A JP7483379B2 (ja) 2019-01-08 2020-01-07 ピペット先端部と一緒に使用するためのピペット
CN202010017287.4A CN111408422B (zh) 2019-01-08 2020-01-08 用于与移液器末端件一起使用的移液器

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP19150847.2A EP3680017B1 (fr) 2019-01-08 2019-01-08 Pipette destinée à une utilisation avec une pointe de pipette

Publications (2)

Publication Number Publication Date
EP3680017A1 EP3680017A1 (fr) 2020-07-15
EP3680017B1 true EP3680017B1 (fr) 2021-07-21

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EP19150847.2A Active EP3680017B1 (fr) 2019-01-08 2019-01-08 Pipette destinée à une utilisation avec une pointe de pipette

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Country Link
US (1) US11554367B2 (fr)
EP (1) EP3680017B1 (fr)
JP (1) JP7483379B2 (fr)
CN (1) CN111408422B (fr)
PL (1) PL3680017T3 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3831486A1 (fr) 2019-12-06 2021-06-09 Eppendorf AG Famille de pointes de pipette comprenant les pointes de pipette destinées à l'utilisation avec des pipettes d'une famille de pipettes et famille de pipettes comprenant les pipettes destinées à l'utilisation avec des pointes de pipette d'une famille de pointes de pipette
JP7315625B2 (ja) * 2020-07-14 2023-07-26 エッペンドルフ エスイー 一体型プランジャを備えたピペット・チップとともに使用するためのピペット
GB2607893A (en) * 2021-06-11 2022-12-21 Spt Labtech Ltd Pipette tip for a liquid dispensing apparatus
CN117085756B (zh) * 2023-09-28 2024-04-02 康容生物科技(太仓)有限公司 一种外置活塞移液器定量吸头及管体

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US4616514A (en) * 1983-06-06 1986-10-14 Rainin Instrument Co., Inc. Replaceable tip assembly for pipette
CH671526A5 (fr) * 1985-12-17 1989-09-15 Hamilton Bonaduz Ag
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JP7315625B2 (ja) * 2020-07-14 2023-07-26 エッペンドルフ エスイー 一体型プランジャを備えたピペット・チップとともに使用するためのピペット

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JP2020110794A (ja) 2020-07-27
US20200215528A1 (en) 2020-07-09
JP7483379B2 (ja) 2024-05-15
PL3680017T3 (pl) 2022-02-07
EP3680017A1 (fr) 2020-07-15
US11554367B2 (en) 2023-01-17
CN111408422B (zh) 2023-02-07
CN111408422A (zh) 2020-07-14

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