EP3831487B1 - Pipette à utiliser avec une pointe de pipette et pointe de pipette à utiliser avec une pipette - Google Patents

Pipette à utiliser avec une pointe de pipette et pointe de pipette à utiliser avec une pipette Download PDF

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Publication number
EP3831487B1
EP3831487B1 EP19214100.0A EP19214100A EP3831487B1 EP 3831487 B1 EP3831487 B1 EP 3831487B1 EP 19214100 A EP19214100 A EP 19214100A EP 3831487 B1 EP3831487 B1 EP 3831487B1
Authority
EP
European Patent Office
Prior art keywords
pipette
tip
rod
clamping
plunger
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Application number
EP19214100.0A
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German (de)
English (en)
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EP3831487A1 (fr
Inventor
Matthias KUNSCH
Jens Wilmer
Sönke BECKER
Burkhardt Reichmuth
Holger Link
Peter Molitor
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eppendorf SE
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Eppendorf SE
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Priority to EP19214100.0A priority Critical patent/EP3831487B1/fr
Publication of EP3831487A1 publication Critical patent/EP3831487A1/fr
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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0478—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure pistons

Definitions

  • the invention relates to a pipette for use with a pipette tip and a pipette tip for use with a pipette.
  • the liquids can in particular be homogeneous (single-phase) liquids consisting of a single liquid component or a homogeneous mixture of several liquid components (solutions). Furthermore, the liquids can be heterogeneous (multi-phase) mixtures of a liquid with another liquid (emulsions) or a solid (suspensions).
  • Pipettes have a stem-shaped pipette housing with a pin (approach) at the bottom for clamping a pipette tip.
  • the pin is often a conical, cylindrical or partially conical and cylindrical projection and is also referred to as a “working cone”.
  • a pipette tip is a hollow tube with a tip opening at the bottom and an attachment opening at the top, with which the pipette tip can be clamped onto the cone. The liquid is absorbed into the pipette tip and released from it. The intake and delivery of the liquid is controlled using the pipette.
  • Fixed volume pipettes are used for pipetting constant volumes. With variable pipettes, the volume to be dosed can be adjusted.
  • a mechanical counter is used to display the set volume.
  • the stroke of a drive device can be adjusted using an adjusting device that is coupled to the counter.
  • the pipette tip is removed from the base after use and can be replaced with a fresh pipette tip be replaced. This allows cross-contamination to be avoided during subsequent pipetting.
  • Air cushion pipettes have a piston-cylinder system in the pipette housing, which is connected via a channel to a through hole in the spigot.
  • Pipette tips for air cushion pipettes do not have an integrated piston.
  • By displacing the piston in the cylinder by means of the drive device an air cushion is moved in order to suck liquid into and eject liquid from a pipette tip clamped onto the pin.
  • the disadvantage of air cushion pipettes are dosing errors due to changes in the length of the air cushion due to the weight of the liquid sucked in and changes in temperature, air pressure and humidity. Contamination of the pipette by aerosols can also be problematic.
  • Air cushion pipettes are offered in different sizes, each with just one matching air cushion pipette tip with a certain nominal volume or several matching air cushion pipette tips with different nominal volumes that are close together. It is well known that air-cushion pipettes and air-cushion pipette tips are marked with a color code to make correct assignment easier. There is a risk of misinterpretation by the user, so that he or she uses the wrong combination of a pipette and a pipette tip.
  • Direct positive displacement pipettes are used with pipette tips with an integrated piston (direct positive displacement pipette tips).
  • This type of pipette has a pin for attaching the pipette tip and a drive device that can be coupled to the integrated piston (tip piston) for moving the piston.
  • the piston comes into direct contact with the liquid, eliminating the adverse effects of an air cushion.
  • Positive displacement pipettes are particularly suitable for dosing liquids with high vapor pressure Viscosity or high density and applications in molecular biology where freedom from aerosols is important to avoid contamination.
  • Air cushion and positive displacement pipette tips for single use or reuse are made of plastic or glass.
  • the drive device has a lifting rod for displacing a piston in a pipette tip, which has a hollow lower lifting rod part and an upper lifting rod part inserted from above into the lower lifting rod part.
  • the upper lifting rod part is connected to a control element which protrudes from an upper end of the pipette housing.
  • a Mastertip ® pipette tip from Eppendorf AG with a nominal volume of 20 ⁇ l can be clamped onto a pipette spigot.
  • the control element By pressing the control element, the lifting rod can be moved downwards so that an upper end of the piston rod of a tip piston of the pipette tip is pressed into the lower lifting rod part.
  • a spring device When the lifting rod is moved downwards to a lower stop, a spring device is preloaded. After the control element is relieved, the spring device moves the lifting rod up to an upper stop, whereby the tip piston is taken along and liquid can be sucked into the pipette tip. The sucked-in liquid can be released by pressing the control element again until it reaches the bottom stop.
  • the user To release the pipette tip, the user must press the control element with increased force so that another spring device compresses, the upper lifting rod part is displaced downwards in the lower lifting rod part, the piston is pushed out of the lower lifting rod part and the pipette tip is pushed off the pin.
  • the Biomaster ® pipette and the Mastertip ® pipette tips are designed for pipetting in the volume range from 1 ⁇ l to 20 ⁇ l.
  • the pipette tip When pipetting highly viscous liquids, the pipette tip can become detached from the pipette. In particular, when sucking in If highly viscous liquids enter the pipette tip, the lifting rod can be pulled off the piston rod.
  • the US 9,931,626 B2 and the FR 3 012 883 A1 describe a pipette system that includes a pipette and a pipette tip with an integrated tip plunger.
  • the pipette has a pin onto which a tip cylinder of the pipette tip can be clamped.
  • the pipette has a gripping device with gripping pliers for clamping the upper end of the tip piston.
  • the upper end of the tip piston includes an insertion bevel at the upper end of a cylindrical rod section.
  • the gripping device can be moved downwards with the gripping tongs open into a position in which the gripping tongs are automatically closed so that they hold the upper end of the tip piston.
  • the gripping device has a locking of the gripping tongs in the open state, which includes a control element which is displaceably arranged within the gripping tongs.
  • the automatic switching from the open to the closed state is triggered by a displacement of the control element, which is brought about by the control element resting on the upper end of the tip piston when the gripping device is moved downwards.
  • the gripper is pressed into the open state by a spring.
  • the gripping device is structurally complex.
  • the tip of the pipette must first be pressed into the tip cylinder and then the tip piston must be connected to the gripping device by pressing a control button downwards.
  • the control element To suck liquid into the pipette tip, the control element must be kept pressed from the time the pipette tip is clamped until the tip opening is immersed in the liquid. If the control element is released after clamping the pipette tip without absorbing liquid, it must be pressed again before sucking in liquid.
  • the EP 0 078 724 A1 describes a positive displacement pipette with a lifting rod with elastic arms at the lower end for clamping the upper end of a piston of a pipette tip.
  • the pipette tip has a tip piston that has an insertion bevel at the upper end and a cylindrical rod section directly adjacent to it.
  • the EP 2 574 402 B1 and the DE 199 48 818 A1 describe syringes with a coding and dosing devices with sensors that scan the coding of the inserted syringe and output a display that depends on the type of syringe.
  • the syringes are held by gripping levers or pawls on a syringe flange of the syringe barrel and grooves in the syringe plunger and are not clamped onto a pin and clamped into a hollow lifting rod like the direct displacement pipette tips.
  • the EP 691 158 B1 describes a pipetting system with a pipetting device and various interchangeable syringes which are coded by radial projections on the piston head of the piston.
  • the pipetting device scans the coding of the syringe attached to the pipetting device using light barriers and automatically identifies the coding.
  • the DE 199 15 066 C2 describes a pipetting system in which the pipetting device detects a reference point and a coding on the piston head during a relative movement between the piston head and a detection device with at least one light barrier.
  • the US 4,563,907 describes an automatic machine for dispensing and diluting fluids using interchangeable syringe systems, which recognizes the syringe type via the maximum extension length of the syringe plunger.
  • the DE 199 15 771 C1 describes a system for receiving and dispensing fluid volumes with a syringe holder and a syringe.
  • the syringe plunger includes an information carrier about the type, condition and/or purpose of the syringe, and a syringe plunger receptacle of the syringe holder has an information reader adapted to the information carrier.
  • the information carrier is formed by radial projections on the piston head, which press on microswitches of the information reader.
  • the DE 199 48 818 C2 describes another system for receiving and dispensing fluid volumes, in which the information is transmitted through an axially aligned recess is formed, which is arranged centrally in the front of the syringe plunger and is detected by the information reader.
  • the known systems use sensors or buttons to scan codes on the piston of the syringes. This is structurally complex and can lead to errors in scanning.
  • the invention is based on the object of creating a direct displacement pipette that enables a secure and easy-to-produce connection to a direct displacement pipette tip. Furthermore, the invention is based on the object of creating a direct displacement pipette tip which enables a secure and easy-to-produce connection to a direct displacement pipette.
  • the tip piston is firmly fixed in the lifting rod by the clamping spring.
  • at least one leg of the clamping spring projects into the axial bore of the lifting rod.
  • the compressive force of the clamping spring acts on the piston rod.
  • a circumferential insertion bevel is provided on it. Due to the insertion bevel, the clamping spring is spread when the piston rod is inserted into the axial bore. The clamping spring then acts on the piston rod with a correspondingly high pressure force. This greatly increases the static friction on the piston rod and securely fixes it in the lifting rod.
  • the tip piston can have a clamping groove in which the clamping spring engages. This creates a positive fit and a high holding force.
  • the elastically deformable clamping spring in conjunction with the insertion bevel and the clamping groove ensure that the piston is clamped to the lifting rod as simply as possible.
  • the insertion bevel and clamping groove are combined, the insertion bevel allows the clamping spring to be easily inserted into the clamping groove.
  • the clamping of the clamping device on the piston rod is clearly evident in a change in the clamping force acting on the piston rod when the lifting rod is pushed on.
  • the significant change in the clamping force is due to the fact that, according to the spring curve of the clamping spring, the clamping force clearly depends on the deformation of the clamping spring Clamping spring depends and strong changes in the clamping force are possible with a clamping spring. This indicates that the clamping device securely clamps the piston rod in the lifting rod.
  • the user receives tactile feedback and with electrically driven pipettes, clamping can be monitored by checking the course of the clamping force when clamping the pipette tip.
  • the pipette and the pipette tip enable the piston rod to be securely fastened in the lifting rod with little construction effort.
  • the force required to clamp the pipette tip to the pipette can be kept low by the clamping spring and the insertion bevel and the clamping groove. Clamping the piston rod in the lifting rod can be done at the same time as clamping the tip cylinder onto the pipette spigot. For this it is not necessary to additionally move the lifting rod downwards.
  • the pipette can be ready for pipetting immediately after the pipette tip has been clamped on. To absorb liquid into the clamped pipette tip, you only need to move the lifting rod upwards.
  • the clamping slot is aligned perpendicular to the axial bore of the lifting rod, i.e. its longitudinal axis extends perpendicular to the axial bore of the lifting rod. In this way, in particular, a precise alignment of the clamping spring with a circular clamping groove of the piston rod is achieved.
  • the lifting rod has two diametrically opposed clamping slots on the outer circumference and a clamping spring which engages with two legs in the clamping slots, projects into the axial bore and sits between the two legs on the outer circumference of the lifting rod.
  • a particularly secure connection to the pipette with the pipette tip can be achieved, since the Clamping spring clamps the piston rod between both legs.
  • the two legs preferably engage from different sides in a clamping groove in the piston rod.
  • the lifting rod has a circumferential clamping spring groove on the outer circumference, the clamping slot is located at the base of the clamping spring groove and the clamping spring sits in the clamping spring groove on the outer circumference of the lifting rod.
  • the clamping spring is additionally secured to the lifting rod by the clamping spring groove.
  • the lifting rod has a clamping spring groove with two diametrically opposed clamping slots at the base, the clamping spring has two legs and both legs engage in the clamping slots.
  • the clamping spring has a U-shape.
  • the curved bridging section of the U-shape sits on the outer circumference of the lifting rod and connects the two legs together. At least one of the legs engages in a clamping slot. Both legs preferably engage in clamping slots.
  • the clamping spring has an inclining U-shape, so that the distance between the two legs decreases towards the free ends of the legs. Thanks to the falling U-shape, the clamping spring can be secured against slipping sideways from the lifting rod.
  • the clamping spring is a snap ring or another locking ring.
  • the clamping spring is bent from a spring steel wire.
  • the clamping spring is punched out of a sheet of spring steel.
  • the drive device of the pipette is a manually drivable, mechanical drive device. This drive device is driven manually by the user of the pipette during pipetting.
  • the mechanical drive device has a Pipette housing protruding control element which can be displaced relative to the pipette housing in the axial direction of the lifting rod and is coupled to the lifting rod. The pipette is powered by manually operating the control element.
  • the drive device has a transmission mechanism coupled to the lifting rod and the operating element, which is designed to shift the lifting rod alternately upwards and downwards during successive downward displacements of the operating element, between which the operating element is displaced upwards.
  • This embodiment is advantageous for an operating element that can be displaced in the longitudinal direction of the pin to drive the drive device.
  • the lifting rod is in a lower position.
  • the control element is moved downwards for the first time, the lifting rod is moved up from the lower position to the upper position and liquid can be sucked into the pipette tip.
  • the control element is subsequently moved upwards, the lifting rod maintains its upper position.
  • the control element is subsequently moved downwards, the lifting rod is moved back to the lower position and the liquid absorbed can be released. This sequence can be repeated as often as desired.
  • the lifting rod can be moved to the lower position and the piston rod clamped when the control element is first moved downwards.
  • the control element is subsequently moved upwards, the lifting rod maintains its lower position. Only when the control element is subsequently moved downwards is the lifting rod moved upwards and liquid can be sucked into the pipette tip. The further process until the liquids are dispensed is as in the example above.
  • the drive device is an electrically driven drive device.
  • This drive device includes an electric motor that drives the lifting rod.
  • the electric motor is coupled to the lifting rod via a transmission mechanism.
  • the transmission mechanism is a mechanism that converts the rotary movements generated by the electric motor into a linear movement of the lifting rod.
  • the electrically driven drive device comprises an electronic control device coupled to the electric motor, wherein the electronic control device is designed to measure the force for the displacement of the lifting rod and, based on changes in the force, to determine which pipette tip is from a pipette tip family comprising different types of pipette tips , which are characterized by piston rods of different heights, are clamped onto the piston rod by pushing the lifting rod with the axial bore and the clamping device.
  • This embodiment takes advantage of the fact that when the lifting rod is pushed onto the piston rod, variable frictional forces are effective between the lifting rod and the piston rod. As already mentioned, the changes in friction forces are particularly pronounced due to the clamping spring.
  • the course of the force to be applied to displace the lifting rod depends on the length of the rod section of the piston rod protruding upwards from the clamping groove.
  • the electronic control device determines the tip type of the pipette tip clamped onto the pipette by evaluating the course of the force for displacing the lifting rod.
  • the electronic control device is designed such that the lifting rod is arranged in an upper position before a pipette tip is clamped on.
  • the tip cylinder is first clamped onto the pin.
  • the piston rod can then be clamped in the lifting rod by pushing the lifting rod onto the piston rod.
  • the lifting rod can be automatically moved to the upper position after switching on the pipette and/or after ejecting a pipette tip.
  • the force for displacing the lifting rod is measured starting from a defined starting point from which the lifting rod moves towards the piston rod for coupling.
  • the impact of the lifting rod on the upper end of the piston rod can be detected by the first increase in the force for displacing the lifting rod and further information can be obtained for determining the tip type.
  • the current consumption of the electric motor when the lifting rod is displaced is measured as a measure of the force for displacing the lifting rod.
  • An increase in force is associated with a corresponding increase in power consumption.
  • the electronic control device is designed to measure the force for displacing the lifting rod when the pipette tip is thrown off the pipette. By evaluating the force curve when the pipette tip is ejected, it can be determined that the pipette tip was ejected correctly from the pipette. This is particularly advantageous for monitoring the operation of remote-controlled or automatic devices Pipettes and pipettes in remote-controlled or automatic systems.
  • the pipette is a single-channel pipette.
  • the single-channel pipette has only a single pin and a single lifting rod for use with a single pipette tip.
  • the pipette is a multi-channel pipette.
  • a multichannel pipette has multiple lugs and cones for simultaneous use with multiple pipette tips.
  • the pipette is a hand pipette. This is a pipette that can be held and operated by the user with one hand when pipetting.
  • the pipette has a stem-shaped pipette housing.
  • a stem-shaped pipette housing is particularly advantageous if the pipette is designed as a hand pipette, since the stem-shaped pipette housing is convenient for handling by the user.
  • the pin is arranged at the lower end of the pipette housing.
  • the pipette is part of an automatic dosing machine or a laboratory machine.
  • the pin has a specific shape.
  • the pin is a conical, cylindrical, spherical (e.g. barrel-shaped) or sectionally conical and/or cylindrical and/or spherical projection.
  • the pin has several conical sections with different cone angles.
  • the pin can have at least one cylindrical section.
  • the second rod section has a clamping groove for engaging the at least one leg the clamping spring and the positive locking of the tip piston on the lifting rod.
  • the piston rod is cylindrical, preferably circular cylindrical.
  • the first rod section and the second rod section are circular cylindrical.
  • the insertion bevel is conical.
  • the tip piston has a disk arranged between the piston and the clamping groove and / or the second rod section and the tip cylinder has a circumferential shoulder or ribs below the seating area on the inner circumference, on which the disk rests in the tip cylinder in the lowest position of the tip piston rests on its underside.
  • the shoulder is the transition between a cylinder section with a larger inner diameter to a cylinder section arranged underneath with a smaller inner diameter of the tip cylinder.
  • the ribs protrude radially from the inner circumference into the interior of the tip cylinder, extend in the axial direction of the tip cylinder and the disk sits with its underside on the upper end of the ribs in the lowest position of the tip piston.
  • the tip piston By placing the disk on the shoulder or the ribs, the tip piston is supported in a defined position and prevents the tip piston from getting stuck in the tip cylinder when the lifting rod is clamped onto the piston rod. Furthermore, the disk can serve to center the tip piston so that the piston rod does not rest on the wall of the cylinder and can be gripped by clamping the lifting rod.
  • the tip piston is needle-shaped.
  • This type of execution is particularly suitable for pipette tips Smaller nominal volume is advantageous, in which the tip piston comprises a needle-shaped piston, which is guided in a sealing manner in the piston running area. Without the support of the tip piston via the disk on the shoulder or the ribs, the needle-shaped piston could be clamped in the piston running area due to its small conicity when the lifting rod is clamped onto the piston rod.
  • the tip piston has a conical section below the disk, which is supported with its outer circumference on the inner diameter of the circumferential shoulder or the ribs when the disk sits on the shoulder or the ribs. This additionally centers the tip piston in the tip cylinder. This makes it easier to push the lifting rod onto the piston rod.
  • the tip piston has a conical, downwardly tapering piston at the lower end, which, in the lowest position of the tip piston in the tip cylinder, is supported on a conical tip section at the lower end of the tip cylinder. This supports the tip piston in a defined position in the tip cylinder and prevents the tip piston from getting stuck in the tip cylinder when clamping the lifting rod.
  • This type of design is particularly advantageous for pipette tips with larger nominal volumes.
  • the tip piston has a disk below the clamping groove and/or the second rod section and above the conical piston, the outer circumference of which is supported on the inner circumference of the tip cylinder.
  • the disc also centers the tip piston in the tip cylinder. This makes it easier to push the lifting rod onto the piston rod.
  • the tip piston has a disk and a conical piston, with the bottom of the disk resting on the shoulder or on the ribs in the lowest piston position and the conical piston sitting on the conical tip section of the tip cylinder.
  • the tip piston has a conical section below the disk, which additionally centers the tip piston on the inner diameter of the circumferential shoulder or the ribs.
  • the inside diameter of the circumferential shoulder is the inside diameter of the cylinder portion of the tip cylinder that extends downward from the shoulder.
  • the inside diameter of the ribs is the inside diameter of a circle centered on the center axis of the tip cylinder that contacts the ribs on the inside edge on the inside.
  • the tip piston has a stop below the clamping groove and/or the second rod section for support on the edge of the hole at the lower end of the lifting rod.
  • the stop prevents the piston rod from being pressed so deeply into the receptacle of the lifting rod that liquid absorbed by the pipette tip cannot be completely released. Without the stop, this would occur, for example, when highly viscous liquid is pipetted.
  • a high force acts on the piston due to the high flow resistance of the liquid, which could push it deeper into the receptacle. This is prevented by placing the stop on the edge of the hole at the lower end of the lifting rod. This avoids pipetting errors.
  • the piston has a tip below the sealing area which has a steeper angle than the tip of the cylinder.
  • the seating area of the pipette tips has a bead running around the inner circumference of the tip cylinder for snapping with an annular groove of the pin running around the outer circumference of the pin of a pipette and/or the seating area of the pipette tips has an annular groove running around the inner circumference for snapping a bead running around the outer circumference of the cone of a pipette.
  • the pipette tip consists of one or more plastics. According to a further embodiment, the pipette tip consists of polypropylene and/or polyethylene, optionally with additives. According to a further embodiment, the tip cylinders are made of polypropylene and the tip pistons are made of polyethylene or PET.
  • the invention relates to a family of pipette tips comprising pipette tips of different tip types according to one of claims 9 to 15 or according to one of the embodiments of the pipette tips additionally described above, the piston rod protruding upwards with respect to the clamping groove or the insertion bevel by a height that is characteristic of the respective tip type.
  • the pipette tips of different tip types differ in the height of the piston rod with respect to the clamping groove or the insertion bevel.
  • the tip type of the pipette tip can therefore be determined based on the height of the piston rod with respect to the clamping groove or the insertion bevel.
  • pipette tips of different tip types differ from one another by one or more of the following features: nominal volume, shape, dimensions, material, coating Surface, with or without treatment of the surface, electrically insulating or electrically conductive, degree of purity. According to a further embodiment, the pipette tips of different tip types have different nominal volumes.
  • the invention relates to a pipette system comprising a pipette according to one of claims 1 to 8 or according to one of the previously described additional embodiments of the pipette and a pipette tip according to one of claims 9 to 15 or according to one of the previously described additional embodiments of the pipette tip or a family of pipette tips Claim 16 or according to one of the previously described additional embodiments of the pipette tip family.
  • the information refers to “top” and “bottom” as well as “vertical” and “horizontal” and terms derived therefrom such as: “above” and “below”, “upright” and “upside down” and “on top of each other” refer to an arrangement of the pipette in which the spigot is vertically aligned and is located at the downward-pointing end of the pipette housing.
  • this information refers to a vertical alignment of the central axis of the pipette tips, with the tip opening at the bottom and the attachment opening at the top.
  • a pipette 1 designed as a direct displacement pipette has a rod-shaped (eg cylindrical) pipette housing 2.
  • a hollow cylindrical shaft 3 projects downwards from the lower end of the pipette housing 2.
  • a pin 4 projects downwards from the lower end of the shaft 3, according to Fig. 1 and 4 has a through hole 5 with a through hole at the lower end. The inside diameter of the through hole 5 is smaller than the inside diameter of the shaft 3.
  • the pin 4 has an upper pin section 6 with the shape of a hollow cone and underneath a lower pin section 7 with the shape of a hollow cone.
  • the upper pin section 6 has a smaller cone angle than the lower pin section 7.
  • An annular groove 8 runs around the outer circumference of the pin 4 between the upper pin section 6 and the lower pin section 7.
  • the upper pin section 6, the annular groove 8 and the lower pin section 7 form first means for positively connecting the pipette to a pipette tip.
  • the annular groove 8 forms a clamping area 9 for a corresponding bead of a pipette tip.
  • the pin 4 has slots 10, 11 running in its longitudinal direction, preferably three, which are diametrically opposed to one another.
  • the slots 10, 11 extend from the lower end over the entire length of the pin 4.
  • a drive device 12 in the pipette housing 2 which includes a transmission element 13 in the form of a transmission rod 14, a transmission mechanism 15 and a drive element 16 in the form of a lifting rod 17. Furthermore, the drive device 12 includes an operating element 18 in the form of an operating lever 19, which is firmly connected to a support plate 21 via a web 20.
  • the support plate 21 has an oval shape with a wide rounded end and a narrow rounded end, with the operating lever 19 protruding from the edge of the narrow rounded end.
  • the carrier plate 21 has a first curved slot 22, which runs approximately parallel to the contour of the narrow, rounded end.
  • the carrier plate 21 has a rectangular first edge recess 23 in the middle of the first curved slot 22 on the side of the narrow rounded end.
  • the lifting rod 17 is guided from above into the shaft 3 and the pin 4.
  • Fig. 4 it is hollow and provided with an axial bore 24 which extends to a hole 25 in the lower end of the lifting rod 17.
  • Fig. 4 has two opposite longitudinal slots 26, which extend from its upper end in the longitudinal direction and end in front of its lower end.
  • the lower end of the lifting rod 17 forms a receptacle 27 for the upper end of a piston rod.
  • the lifting rod can also be provided with only a single longitudinal slot 26, so that it has a C-shaped cross section.
  • the lifting rod 17 has a circumferential clamping spring groove 28 in the unslotted area next to its lower end on the outer circumference.
  • clamping slots 29.1, 29.2 are clamping slots 29.1, 29.2 on two diametrically opposite sides in the lifting rod 17, in which the clamping spring groove 28 is located axial bore 24 of the lifting rod 17 is open.
  • Each clamping slot 29.1, 29.2 extends from the bottom of the clamping spring groove 28 to the axial bore 24.
  • the longitudinal axes of the clamping slots are aligned perpendicular to the axial bore 24 and parallel to one another.
  • the clamping spring groove 28 and the clamping slots 29.1, 29.2 have matching heights, that is, their extension in the longitudinal direction of the lifting rod 17 is the same.
  • a clamping spring 30 is inserted into the clamping spring groove 28 and engages through the clamping slots 29.1, 29.2 into the inner cross section of the lifting rod 17.
  • the clamping spring 30 has a U-shape.
  • the bridging section 30.1 of the U is bent according to the clamping spring groove 28.
  • the clamping spring 30 is bent from a spring steel wire.
  • the diameter of the wire corresponds to the height of the clamping spring groove 28 and the clamping slots 29.1, 29.2 or is slightly less than this.
  • the two legs 30.2, 30.3 extend in the clamping slots 29.1, 29.2 and protrude into the axial bore 24. According to Fig. 8 the two legs 30.2, 30.3 run like chords of a circle through opposite edge regions of the axial bore 24. The incident U-geometry prevents the clamping spring 30 from emerging laterally from the clamping spring groove 28.
  • the transmission mechanism 15 is designed in such a way that during successive displacements of the operating lever 19 downwards, between which the operating lever 19 is displaced upwards, the lifting rod 17 is alternately displaced downwards and upwards. As a result, by pressing the operating lever 19 downwards the lifting rod 17 is moved from an upper position to a lower position, the lifting rod 17 maintains the lower position during the subsequent upward displacement of the operating lever 19 and the lifting rod 17 is displaced upward again by subsequently pressing the operating lever 19 downwards. This can be repeated as often as desired.
  • the pipette 1 is provided with a discharge device 31.
  • This includes a cam carrier 32 which is rotatably mounted in the pipette housing 2 and is designed as a hollow cylindrical rotating sleeve 33.
  • the rotating sleeve 33 is, for example, rotatably mounted with its outer circumference on the inner circumference of the pipette housing 2 and is supported at the upper and lower ends between shoulders of steps on the inner circumference of the pipette housing 2, so that it cannot be displaced in the axial direction in the pipette housing 2.
  • the axis of rotation of the rotating sleeve 33 coincides with the longitudinal axis of the pipette housing 2 and the longitudinal axis of the pin 4.
  • the rotating sleeve 33 has recesses 34, 35 parallel to its axis of rotation on two diametrically opposite sides, which extend from the upper edge of the rotating sleeve 33 and end at a distance from its lower edge. Below the recesses, the rotating sleeve 33 therefore consists of an annular base 36 and above it consists of two diametrically opposed sectors 37, 38 of a circular ring, which laterally delimit the two recesses 34, 35.
  • the first curve 39 is designed as a first groove 41 in the form of an inverted (upside down) Y.
  • the vertical part 42 of the Y extends far upward on a sector 37 to just before the upper edge of the sector 37.
  • the second curve 40 is a second groove 43 on the outer circumference of the base 36 of the rotating sleeve 33 with the shape of an upright standing V.
  • the first curve 39 and the second curve 40 are arranged offset by 90 ° to one another on the circumference of the rotating sleeve.
  • the first curve 39 and the second curve 40 each extend over an angular range of less than 90° over the circumference of the rotating sleeve 33.
  • the discharge device 31 includes a discharge rod which includes a strip-shaped upper discharge rod section 45 and a cylindrical lower discharge rod section 46.
  • a discharge rod which includes a strip-shaped upper discharge rod section 45 and a cylindrical lower discharge rod section 46.
  • the upper and lower discharge rod sections 45, 46 are arranged parallel to one another and laterally offset from one another.
  • the lower end of the upper discharge rod section 45 is connected to the upper end of the lower discharge rod section 46 via a strip-shaped connecting rod section 47 which is inclined obliquely to the two discharge rod sections.
  • a first scanning element 48 in the form of a first guide pin 49 projects vertically from the inside of the upper discharge rod section 45.
  • the discharge rod 44 is preferably formed in one piece, for example made of a rigid plastic or metal.
  • the discharge rod 44 is guided with the first guide pin 49 in the first groove 41, passes through the longitudinal slot 26 of the lifting rod 17 with the connecting rod section 47 and extends with the lower discharge rod section 46 within the lifting rod 17 to just before the lower end of the same.
  • the pipette 1 includes a locking device 50, which includes a locking sleeve 51 and a strip-shaped control rod 52 parallel to it.
  • the upper end of the locking sleeve 51 and the lower end of the control rod 52 are connected to one another via a second connecting rod section 53, which is inclined obliquely to the locking sleeve 51 and to the control rod 52.
  • From the inside of the Control rod 52 projects a second scanning element 54 in the form of a second guide pin 55.
  • the second guide pin 55 is guided in the second groove 43.
  • the locking sleeve 51 is inserted into the shaft 3 from above and rests on the inside of the pin 4.
  • the lifting rod 17 and the discharge rod 44 are inserted into the locking sleeve 51 from above.
  • the operating element 18 is pushed onto the sector 37 of the rotary sleeve 33 with the first curved slot 22, on which the first groove 41 extends.
  • the rotating sleeve 33 is connected at the top to a support ring 56, which bridges the two sectors 37, 38 and stabilizes the rotating sleeve 33.
  • the support ring 56 has a downwardly projecting jacket 57 on the outer edge, which laterally surrounds the two sectors 37, 38 on the outer edges. It also has a second curved slot 58 which receives the upper edge of the sector 38, which is not provided with a groove 41, 43.
  • the support ring 56 is connected to the rotary sleeve 33, for example by gluing and/or in a form-fitting manner.
  • the rotating sleeve 33 and the locking sleeve 51 as well as the operating element 18 are made, for example, from one or more rigid plastics and/or metal.
  • the rotating sleeve 33, the support ring 56, the operating element 18 and/or the locking sleeve 51 are preferably each formed in one piece.
  • a control button of the control element 18 can also be made of an elastic or soft-elastic plastic or rubber.
  • the operating lever 19 stands out of the pipette housing 2 through a first housing slot 60 which runs transversely to the longitudinal axis of the pipette housing 2 and extends over part of the circumference of the pipette housing 2, so that it can be operated from the outside.
  • the first housing slot 60 is connected in the middle to a second housing slot 61 which runs downward in the longitudinal direction of the pipette housing 2.
  • the operating lever 19 can be moved downwards from the support ring 56 along the second housing slot 61 against the action of a spring device, whereby it slides with the first curved slot 22 on the sector 37 of the rotating sleeve 33. After the load has been relieved, the spring device automatically shifts the operating lever 19 back upwards.
  • a sleeve-shaped third scanning element 62 is guided on the outside of the shaft 3.
  • a spring device in the form of a coil spring 63 guided on the shaft is supported on the underside of the pipette housing 2 and on the top of the third scanning element 62.
  • the third scanning element 62 is pressed from above against a stop element on the shaft 3 or pin 4 by the coil spring 63.
  • An adjustment button 64 for setting a dosing volume is arranged on the top of the pipette housing 2.
  • the dosing volume can be adjusted by turning the adjustment knob 64.
  • a counter 65 arranged underneath in the pipette housing 2 shows the dosing volume set.
  • the adjustment button 64 and/or the counter 65 is coupled to the transmission mechanism 15.
  • the transmission mechanism 15 is designed to change the stroke of the lifting rod 17 in accordance with the set dosing volume, which is carried out by moving the operating element 18 downwards.
  • a pipette tip 66 is placed on the pin 4 and includes a tip cylinder 67 and a tip piston 68.
  • the top cylinder has 67 a tubular body 69 which has a tip opening 70 at the lower end, a collar 72 having an insertion opening 71 at the upper end and, on the inner circumference of the collar 72, a connecting region 73 for clamping onto the pin 4.
  • the connecting area 73 has a contour complementary to the pin 4, which has a conical lower connecting section 74 at the bottom for receiving the conical lower pin section 7, above it a circumferential bead 75 for engaging in the annular groove 8 of the pin 4 and above it a conical upper connecting section 76 for receiving the cylindrical upper pin section 6.
  • the lower connecting section 74, the bead 75 and the upper connecting section 76 form second means for positively connecting the pipette tip 66 to the pipette 1.
  • the tubular body 69 has a cylindrical piston running area 77. Below this, the tubular body 69 has a downwardly tapering tip section 78 with the shape of a hollow truncated cone.
  • the tip section 78 is in Fig. 4 shown and omitted from the remaining drawings for reasons of simplicity.
  • the tip piston 68 is inserted into the tubular body 69.
  • This includes a piston 79, which is guided in the piston running area 77.
  • a piston rod 80 which has a smaller diameter than the piston 79, projects upward from the piston 79.
  • the piston rod 80 has a cylindrical first rod section 81 at the top and a cylindrical second rod section 82 underneath with a larger diameter than the first rod section 81.
  • the piston gear 80 has a cylindrical third rod section 83 with the same diameter as the first rod section 81.
  • the piston rod 80 has a circumferential insertion bevel 84.
  • the diameter gradually expands from the diameter of the first rod section 81 to the diameter of the second rod section 82.
  • the insertion bevel 84 is conical.
  • the piston rod 80 has an externally circumferential clamping groove 85.
  • a pipette tip family includes five different pipette tip types. These are pipette tips 66.1 to 66.5 with different nominal volumes of 10 ⁇ l ( Fig. 15a ), 25 ⁇ l ( Fig. 15b ), 100 ⁇ l ( Fig. 15c ), 250 ⁇ l ( Fig. 15d ) and 1000 ⁇ l ( Fig. 15e ). For all pipette tips 66.1 to 66.5 from Fig. 15 the tip piston 68.1 to 68.5 is in a position in which it is pushed as deeply as possible into the tip cylinder 67.1 to 67.5.
  • the tip piston 68.1 to 68.3 can be inserted into the tip cylinder 67.1 to 67.3 for the smaller pipette tips 66.1 to 66.3 with a nominal volume of 10, 25 and 100 ⁇ l by seating a tip piston 68.1 to 68.3 between the clamping groove 85.1 to 85.3 and the piston 79.1 to 79.3 arranged disk 86.1 to 86.3 on a shoulder 87.1 to 87.3 in the tip cylinder 67.1 to 67.3.
  • the support of the tip pistons 68.1 to 68.3 via the disks 86.1 to 86.3 on the shoulders 68.1 to 68.3 prevents the slim or needle-shaped pistons 79.1 to 79.3 from sliding into the piston running areas 77.1 to 77.3 when the lifting rod 17 is pushed onto the piston rods 80.1 to 80.3 be clamped.
  • the tip pistons 68.1 to 68.3 have a first cone section 88.1 to 88.3, which rests on the inner circumference of the shoulder 87.1 to 87.3 and centers the tip piston 68.1 to 68.3 in the tip cylinder 67.1 to 67.3.
  • the two larger pipette tips 66.4, 66.5 with a nominal volume of 250 ⁇ l and 1000 ⁇ l also have a disc 86.4, 86.5.
  • the tip pistons 68.4, 68.5 are centered in the tip cylinder 67.4, 67.5.
  • the lowest position of the tip piston 68.3 to 68.5 is defined by placing a conical piston 79.3 to 79.5 on a conical tip section 78.3 to 78.5 at the lower end of the tip cylinder 67.3 to 67.5.
  • the different types of pipette tips are characterized by piston rods of different heights 80.1 to 80.5.
  • the height of the piston rods is the length by which the piston rods 80.1 to 80.5 protrude upwards in the vertical direction with respect to the clamping groove 85.1 to 85.5.
  • the pipette tip 66 from Fig. 4 Essentially corresponds to the pipette tip 66.4 from Fig. 15d .
  • the pipette tip 66.4 from Fig. 15d is a further development of the pipette tip 66 from Fig. 4 with additional details.
  • the pipette tips from Fig. 15 are like the pipette tips 66 from Fig. 4 clamped onto the pipette 1.
  • Fig. 4 is the bead 75 is snapped to the annular groove 8.
  • the bead 75 forms a seating area 89 which is clamped onto the clamping area 9 of the pipette 1.
  • the clamping spring 30 is snapped into the clamping groove 85.
  • the pipette tip 66 is securely fixed to the pipette 1.
  • the pipette tip 66 can be separated from the pipette 1 by pushing the piston rod 80 out of the lifting rod 17 by moving the discharge rod 44 downwards and pressing the tip cylinder 67 off the pin 4.
  • Fig. 16 The respective tip type can be detected due to the different heights of the piston rods 80.
  • the principle is carried out Fig. 16 illustrated. According to Fig. 16a the pipette 1 is first brought into a starting position in which the lifting rod 17 is aligned with the piston rod 80.
  • Fig. 16c In a third phase, the clamping spring 30 slides over the upper end of the piston rod 80. Because of the friction between the clamping spring 30 and the piston rod 80, an increased amount of force is required for this.
  • Fig. 17 The pushing-on forces are illustrated by the measured motor current when pushing on the lifting rod 17.
  • This diagram shows the motor current versus time. This is the curve with the irregular shape. Furthermore, the displacement of the lifting rod 17 over time is shown by a straight line.
  • an area 1 from the left edge to the first vertical line of the idle current of the electric motor is measured.
  • the motor current in the phase when the lifting rod is initially pushed onto the piston rod is indicated in the area 2 between the first and second vertical lines.
  • the motor current increases until the clamping spring 30 hits the upper end of the piston rod 80.
  • the motor current drops again.
  • the motor current is approximately constant at an increased level. This is shown in area 3 between the second and third vertical lines.
  • the respective tip type attached can be determined automatically.
  • the tip type determined can be displayed and/or taken into account when operating the pipette, for example to control the pipette so that a specific volume of liquid is pipetted.
  • Pipette 1 can be used as follows: According to Fig. 1 and 11 A pipette tip 66 is held on the pipette 1 in a pipetting position.
  • the seating area 88 is positively connected to the pin 4 by the engagement of the bead 75 in the annular groove 8.
  • the control element 18 is located in the pipetting position at the upper end of the second housing slot, in which it can be rotated into the first housing slot in both directions. The maximum angle of rotation is limited by the extent of the first and second grooves 41, 43 in the circumferential direction or the first housing slot, whichever is smaller.
  • the locking sleeve 51 is according to Fig. 4 arranged in the lowest position, so that it prevents the pipette tip 66 from being accidentally detached from the pin 4. In order to break the positive connection, a radial narrowing of the pin 4 would be required, which the locking sleeve 51 does not allow in this position.
  • the tip piston 68 is clamped with the upper rod section 82 in the receptacle 27 of the lifting rod 17.
  • the third scanning element 62 is pressed against the upper edge of the pipette tip 66 by the preloaded coil spring 63.
  • the position of the third scanning element 62 depends on the height of the collar 72 of the attached pipette tip 66 or on the length by which the collar 72 protrudes with respect to the bead 75.
  • the height of the collar 72 is characteristic of this Nominal volume of the pipette tip 66 used.
  • the third scanning element 62 is coupled to a diaphragm or another range setting device on the counter 65 via a slide (not shown) or another transmission device. As a result, the counter 65 displays the volume that can be pipetted with the attached pipette tip 66 at the stroke set using the control element 18.
  • the pipette 1 is immersed in a liquid with the lower end of the pipette tip 66 held on it.
  • the control element 18 is then pressed down. This movement is converted by the transmission device 15 into a lifting movement of the lifting rod 17.
  • the tip piston 68 is displaced upward.
  • the first rod section 81 takes the first scanning element 44 with it, so that the first guide pin 49 slides upward in the vertical part 42 of the Y-shaped first groove 41. Meanwhile, the locking sleeve 45 maintains its position. This is in Fig. 12 shown.
  • the pipette tip 66 When the control element 18 has completed the set stroke, the pipette tip 66 is filled with a certain amount of liquid. The control element 18 is then relieved and shifted back upwards by the spring device until it rests on the support ring 56. To dispense this amount of liquid, the pipette 1 can be aligned with the pipette tip 66 towards another vessel. By pressing the control element 18 downwards again, the lifting rod 17 is shifted downwards and the amount of liquid is dispensed. The first guide pin 49 slides down to the junction of the first groove 41.
  • control element 18 When taking in and dispensing liquid, the control element 18 carries out a full operating stroke. The stroke carried out depends on the set amount of liquid.
  • the intake and release of fluid can be carried out several times.
  • the control element 18 is pivoted from the pipetting position to the right or to the left into a ejection position.
  • the rotary sleeve 33 is rotated so that the second groove 43 shifts the second guide pin 55 and thus the locking sleeve 51 upwards until the locking sleeve 51 has released the pin 4 to such an extent that it can be deformed radially inwards.
  • the locking sleeve 51 is preferably pulled out of the through hole 5.
  • the first guide pin 49 is displaced downwards in one of the two lateral sections of the lower part of the first groove 41, so that the first scanning element 44 presses against the tip piston 68, which is supported at the bottom on the tip section 78.
  • the bead 75 exerts a radial force on the pin 4, so that it narrows and the positive connection between the pipette tip 66 and the pin 4 is broken. This causes the pipette tip 66 to be released from the pin 4.
  • the stripping of the pipette tip 66 from the pin 4 can be supported by the fourth scanning element 62, which is pressed against the upper edge of the pipette tip 66 by the prestressed coil spring 63.
  • a new pipette tip 66 can be connected to the pin 4.
  • the pipette 1 with the pin 4 can be inserted into the insertion opening 71 of a pipette tip 66 held ready in a carrier.
  • the third scanning element 62 is shifted upwards and the coil spring 63 is pretensioned.
  • the counter 65 shows the nominal volume of the attached pipette tip 66.
  • the first rod section 81 presses against the underside of the first scanning element 44, so that the first guide pin 49 slides to the first branching point of the first groove 41.
  • the rotating sleeve 33 in the pipette housing 2 is rotated until the control element 18 is in the pipetting position.
  • the second guide pin 55 slides to the low point in the second groove 43. This moves the locking sleeve 51 into the locking position Fig. 4 shifted, in which it prevents the pipette tip 66 from detaching from the pin 4.
  • Connecting the tip piston 68 to the lifting rod 17 and pipetting can be done in the manner described above.
  • a pipette tip 66.6 is shown, which differs from the pipette tip 66.5 of FIG. 17e in that the piston rod 80.6 has a stop 90 below the clamping groove 85.6 at the lower end of the second rod section 82.6.
  • the stop 90 is formed by the top of a second cone section 91 which widens upwards.
  • the stop 90 is formed by the top of a disk or by several outwardly projecting projections distributed at the same height over the circumference of the piston rod 80.
  • the stop 90 is positioned so that the piston rod 80 can be pushed into the receptacle 27 until the clamping spring 30 engages in the clamping groove 85.
  • the piston rod 80 is prevented from being pushed further into the receptacle 27.
  • Pulling the tip piston 68.6 out of the receptacle 27 is only possible by overcoming the clamping force of the clamping spring 30.
  • the tip piston 68 is held immovably by the lifting rod 17 both upwards and downwards. This avoids pipetting errors when collecting and dispensing liquid.

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Claims (16)

  1. Pipette à utiliser avec une pointe de pipette comprenant
    • un boîtier de pipette (2)
    • un tourillon (4) faisant saillie vers l'extérieur à partir du boîtier de pipette (62) pour le serrage d'une zone de siège (89) sur la circonférence intérieure d'un cylindre de pointe (67) d'une pointe de pipette (66) présentant une ouverture de montage (71) sur l'extrémité supérieure, le tourillon (4) présentant un alésage traversant (5) pour l'introduction d'une tige de piston (80) d'un piston de pointe (68) de la pointe de pipette (66),
    • un dispositif d'entraînement (12) comprenant une tige de levage (17) guidée dans le boîtier de pipette (2) alignée sur l'alésage traversant (5) et déplaçable dans la direction longitudinale du tourillon (4) avec un alésage axial (24) et un orifice (25) sur l'extrémité inférieure pour l'introduction de l'extrémité supérieure de la tige de piston (80) et pour le déplacement d'un piston (79) du piston de pointe (68) guidé de façon étanche dans une zone de course de piston (77) entre une ouverture de pointe (70) sur l'extrémité inférieure et la zone de siège (89) du cylindre de pointe (67) et
    • un dispositif de serrage comprenant au moins une encoche de serrage (29.1, 29.2), ouverte vers l'alésage axial, sur la circonférence extérieure de la tige de levage (17) et un ressort de serrage (30) reposant sur la circonférence extérieure de la tige de levage (17), engagé avec un bras (30.2, 30.3) dans l'encoche de serrage (29.1, 29.2) et pénétrant dans l'alésage axial pour le serrage de la tige de piston (80) présentant un biseau d'introduction (84) et/ou une rainure de serrage (85) dans l'alésage axial (24) de la tige de levage (17).
  2. Pipette selon la revendication 1, la tige de levage (17) présentant deux encoches de serrage (29.1, 29.2) diamétralement opposées l'une à l'autre sur la circonférence extérieure et un ressort de serrage (30) engagé dans les encoches de serrage (29.1, 29.2) avec deux bras (30.2, 30.3), pénétrant dans l'alésage axial (24) et reposant sur la circonférence extérieure de la tige de levage (17) entre les deux bras (30.2, 30.3).
  3. Pipette selon la revendication 1 ou 2, la tige de levage (17) présentant, sur la circonférence extérieure, une rainure de ressort de serrage (28) circonférentielle, l'au moins une encoche de serrage (29.1, 29.2) étant disposée sur le fond de la rainure de ressort de serrage (28) et le ressort de serrage (30) reposant dans la rainure de ressort de serrage (28) sur la circonférence extérieure de la tige de levage (17).
  4. Pipette selon l'une des revendications 1 à 3, le ressort de serrage (30) présentant une forme en U inclinée vers l'intérieur.
  5. Pipette selon l'une des revendications 1 à 4, le dispositif d'entraînement (12) étant un dispositif d'entraînement (18) mécanique entraînable manuellement avec un élément de commande (18) faisant saillie à partir du boîtier de pipette (2), déplaçable dans la direction axiale de la tige de levage (17) relativement au boîtier de pipette (2) et accouplé avec la tige de levage (17) ou le dispositif d'entraînement (12) étant un dispositif d'entraînement entraînable électriquement avec un moteur électrique accouplé avec la tige de levage (17) et un dispositif de commande électronique avec une interface utilisateur pour l'utilisation de la pipette.
  6. Pipette selon l'une des revendications 1 à 5, le dispositif d'entraînement (12) présentant une mécanique de transfert accouplée avec la tige de levage (17) et l'élément de commande (18), laquelle est conçue pour déplacer la tige de levage (17) alternativement vers le haut et vers le bas, lors de déplacements successifs de l'élément de commande (18) vers le bas, entre lesquels l'élément de commande (18) est déplacé vers le haut.
  7. Pipette selon la revendication 5, le dispositif de commande électronique étant conçu pour mesurer la force pour le déplacement de la tige de levage (17) et pour déterminer, à l'aide des modifications de la force, quelle pointe de pipette (66) parmi une famille de pointes de pipettes comprenant différents types de pointes de pipettes, laquelle est caractérisée par des tiges de piston de hauteurs différentes, est reliée par enfoncement de la tige de levage (17) avec l'alésage axial (24) et le dispositif de serrage (28, 29, 30) sur la tige de piston (80).
  8. Pipette selon la revendication 7, le courant absorbé par le moteur électrique lors du déplacement de la tige de levage étant mesuré comme valeur pour la force en vue du déplacement de la tige de levage (17) et le type de pointe de pipette étant déterminé à l'aide de l'évolution du courant absorbé du moteur électrique mesurée.
  9. Pointe de pipette à utiliser avec une pipette comprenant
    • un cylindre de pointe (67) et
    • un piston de pointe (68),
    • le cylindre de pointe (67) présentant un corps tubulaire (69) avec une ouverture de pointe (70) sur l'extrémité inférieure, une ouverture de montage (71) sur l'extrémité supérieure, une zone de siège (89) sur la circonférence intérieure pour le serrage sur un tourillon (4) avec un alésage traversant (5) d'une pipette (1) et une zone de course de piston (77) cylindrique entre l'ouverture de pointe (70) et la zone de siège (89),
    • le piston de pointe (68) présentant un piston (79) guidé de façon étanche dans la zone de course de piston (77) et une tige de piston (80) faisant saillie vers le haut pour l'introduction dans l'alésage traversant (5) du tourillon (4) et le serrage dans un alésage axial (24) avec un orifice (25) sur l'extrémité inférieure d'une tige de levage (17) de la pipette (1) alignée sur l'alésage traversant (5) et
    • la tige de piston (80) présentant une première section de tige (81) et une deuxième section de tige (82) sous la première section de tige (81) et un biseau d'introduction circonférentiel (84) entre la première section de tige (81) et la deuxième section de tige (82) et une rainure de serrage (85) circonférentielle sur la circonférence extérieure pour le serrage d'un dispositif de serrage (28, 29, 30) de la pipette (1) avec un ressort de serrage (30) pénétrant dans l'alésage axial (24) de la tige de levage (17) avec au moins un bras (30.1, 30.2) par une encoche de serrage (29.1, 29.2) de la tige de levage (17) et
    • la deuxième section de tige (82) présentant la rainure de serrage (85) pour l'engagement de l'au moins un bras (30.1, 30.2) du ressort de serrage (30) et la fixation par liaison de forme du piston de pointe (68) sur la tige de levage (17).
  10. Pointe de pipette selon la revendication 9, le piston de pointe (68) présentant un disque (86) disposé entre le piston (79) et la rainure de serrage (85) et/ou la deuxième section de tige (82) et le cylindre de pointe (67) présentant un épaulement circonférentiel (87) ou des nervures sous la zone de siège (89) sur la circonférence intérieure, sur lequel ou lesquelles le disque (86) repose avec son côté inférieur dans la position la plus basse du piston de pointe (68) dans le cylindre de pointe (67).
  11. Pointe de pipette selon la revendication 10, le piston de pointe (68) présentant une section conique (88) sous le disque (86), laquelle s'appuie avec sa circonférence extérieure sur le diamètre intérieur de l'épaulement circonférentiel (87) ou des nervures, lorsque le disque (86) repose sur l'épaulement (87) ou les nervures.
  12. Pointe de pipette selon l'une des revendications 9 à 11, le piston de pointe (68) présentant sur l'extrémité inférieure un piston (79) conique se rétrécissant vers le bas, lequel s'appuie sur une section de pointe (78) conique sur l'extrémité inférieure du cylindre de pointe (67) dans la position la plus basse du piston de pointe (68) dans le cylindre de pointe (67).
  13. Pointe de pipette selon la revendication 12, le piston de pointe (68) présentant un disque (86) sous la rainure de serrage (85) et/ou la deuxième section de tige (82) et au-dessus du piston (79), lequel disque s'appuie avec sa circonférence extérieure sur la circonférence intérieure du cylindre de pointe (67).
  14. Pointe de pipette selon l'une des revendications 9 à 13, le piston de seringue (68) présentant, sous la rainure de serrage (85) et/ou la deuxième section de tige (82), une butée pour l'appui sur le bord de l'orifice (25) sur l'extrémité inférieure de la tige de levage (17).
  15. Famille de pointes de pipettes comprenant des pointes de pipettes (1) de différents types de pointes selon l'une des revendications 9 à 14, la tige de piston (80) dépassant vers le haut d'une hauteur caractéristique pour le type de pointe respectif, relativement à la rainure de serrage (85) ou au biseau d'introduction (84).
  16. Système de pipettes comprenant une pipette (1) selon l'une des revendications 1 à 8 et une pointe de pipette (66) selon l'une des revendications 9 à 14 ou une famille de pointes de pipettes selon la revendication 15.
EP19214100.0A 2019-12-06 2019-12-06 Pipette à utiliser avec une pointe de pipette et pointe de pipette à utiliser avec une pipette Active EP3831487B1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP19214100.0A EP3831487B1 (fr) 2019-12-06 2019-12-06 Pipette à utiliser avec une pointe de pipette et pointe de pipette à utiliser avec une pipette

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP19214100.0A EP3831487B1 (fr) 2019-12-06 2019-12-06 Pipette à utiliser avec une pointe de pipette et pointe de pipette à utiliser avec une pipette

Publications (2)

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EP3831487A1 EP3831487A1 (fr) 2021-06-09
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Publication number Priority date Publication date Assignee Title
EP4279182A1 (fr) 2022-05-19 2023-11-22 Eppendorf SE Pipette pour l'utilisation avec une pointe de pipette
CN116764712B (zh) * 2023-08-22 2023-10-20 福建鸿燕化工有限公司 一种醋酸取液器

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Publication number Priority date Publication date Assignee Title
FR2515339A1 (fr) * 1981-10-28 1983-04-29 Marteau D Autry Eric Pipette de precision a deplacement positif
US4563907A (en) 1983-10-31 1986-01-14 Micromedic Systems Inc. Direct reading automatic pipette
ES2163459T3 (es) 1994-07-07 2002-02-01 Brand Gmbh & Co Kg Sistema de pipeteado.
DE19915066C2 (de) 1999-04-01 2001-09-13 Brand Gmbh & Co Kg Verfahren zur Erkennung des Typs von austauschbaren gerätespezifischen Kolben-Zylinder-Einheiten für Pipettier- oder Dosiergeräte sowie Pipettier- und Dosiersystem
DE19915771C1 (de) 1999-04-08 2001-01-04 Retec Elektronische Regeltechn System zum Aufnehmen und Abgeben von Fluidvolumina
DE19948818C2 (de) 1999-10-09 2002-09-05 Retec Elektronische Regeltechn System zum Aufnehmen und Abgeben von Fluidvolumina
DE102011114591B3 (de) 2011-09-30 2012-12-27 Eppendorf Ag Spritze für den Gebrauch mit einer Dosiervorrichtung, Spritzensystem umfassend mehrere Spritzen für den Gebrauch mit einer Dosiervorrichtung, Dosiervorrichtung für den Gebrauch mit einer Spritze, Dosiersystem umfassend eine Spritze und eine Dosiervorrichtung und Verfahren zum Betreiben eines Dosiersystems
FR3012883B1 (fr) 2013-11-07 2015-12-25 Gilson Sas Systeme de pipetage a deplacement positif, presentant une conception facilitant la prehension du piston de l'ensemble capillaire-piston
JP6042562B2 (ja) * 2013-11-29 2016-12-14 ヤマハ発動機株式会社 吸引チップ
EP3560596A1 (fr) * 2018-04-23 2019-10-30 Eppendorf AG Pipette à utiliser avec une pointe de pipette et une famille de pipettes comprenant plusieurs pipettes avec des volumes nominaux différents

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