EP3680016B1 - Pipette destinée à une utilisation avec une pointe de pipette - Google Patents

Pipette destinée à une utilisation avec une pointe de pipette Download PDF

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Publication number
EP3680016B1
EP3680016B1 EP19150808.4A EP19150808A EP3680016B1 EP 3680016 B1 EP3680016 B1 EP 3680016B1 EP 19150808 A EP19150808 A EP 19150808A EP 3680016 B1 EP3680016 B1 EP 3680016B1
Authority
EP
European Patent Office
Prior art keywords
pipette
nipple
pipette tip
housing
pin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
EP19150808.4A
Other languages
German (de)
English (en)
Other versions
EP3680016A1 (fr
Inventor
Florian Tesch
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eppendorf SE
Original Assignee
Eppendorf SE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eppendorf SE filed Critical Eppendorf SE
Priority to PL19150808T priority Critical patent/PL3680016T3/pl
Priority to EP19150808.4A priority patent/EP3680016B1/fr
Priority to JP2020001046A priority patent/JP7458791B2/ja
Priority to CN202010017457.9A priority patent/CN111408423B/zh
Priority to US16/737,483 priority patent/US11426720B2/en
Publication of EP3680016A1 publication Critical patent/EP3680016A1/fr
Application granted granted Critical
Publication of EP3680016B1 publication Critical patent/EP3680016B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/0227—Details of motor drive means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • B01L3/0279—Interchangeable or disposable dispensing tips co-operating with positive ejection means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0286—Ergonomic aspects, e.g. form or arrangement of controls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/023—Adapting objects or devices to another adapted for different sizes of tubes, tips or container
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/08—Ergonomic or safety aspects of handling devices
    • B01L2200/087—Ergonomic aspects
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics

Definitions

  • the invention relates to a pipette for use with a pipette tip.
  • the liquids can in particular be homogeneous (single-phase) liquids, consisting of a single liquid component or a homogeneous mixture of several liquid components (solutions). Furthermore, the liquids can be heterogeneous (multiphase) mixtures of a liquid with another liquid (emulsions) or a solid (suspensions).
  • Pipettes have a stem-shaped pipette housing with a pin (shoulder) at the lower end for clamping on a pipette tip.
  • the pin is often a conical, cylindrical or partially conical and cylindrical projection and is also referred to as a "working cone”.
  • a pipette tip is a hollow tube with a tip opening at the lower end and an attachment opening at the upper end, with which the pipette tip can be clamped onto the pin.
  • the liquid is taken up in the pipette tip and dispensed from it. The uptake and delivery of the liquid is controlled by means of the pipette.
  • Fixed volume pipettes are used to pipette constant volumes. With variable pipettes, the volume to be dispensed can be set.
  • a mechanical counter is used to display the set volume.
  • the stroke of a drive device can be adjusted by means of an adjusting device which is coupled to the counter.
  • the pipette tip is detached from the base after use and can be exchanged for a fresh pipette tip. In this way, cross-contamination can be avoided during subsequent pipetting operations.
  • Air-cushion pipettes have a piston-cylinder system in the pipette housing, which is connected to a through hole in the pin via a channel. Pipette tips for air-cushion pipettes (air-cushion pipette tips) do not have an integrated piston.
  • Positive displacement pipettes are used with pipette tips with an integrated piston (positive displacement pipette tips).
  • This type of pipette has a pin for fastening the pipette tip and a drive device, which can be coupled to the integrated piston (tip piston), for moving the piston.
  • the piston comes in direct contact with the liquid, eliminating the effects of an air cushion.
  • Positive displacement pipettes are particularly suitable for dosing liquids with high vapor pressure, high viscosity or high density and applications in molecular biology where freedom from aerosols is important in order to avoid contamination.
  • Air-cushion or positive displacement pipette tips for single use or for re-use are made of plastic or glass.
  • the drive device has a lifting rod for moving a piston in a pipette tip, which has a hollow lower lifting rod part and an upper lifting rod part inserted into the lower lifting rod part from above.
  • the upper lifting rod part is connected to an operating element which protrudes from the upper end of the pipette housing.
  • a Mastertip® pipette tip from Eppendorf AG is can be clamped onto a pin of the pipette. By pressing the operating element, the lifting rod can be moved downwards so that an upper end of the piston rod of a tip piston of the pipette tip is pressed into the lower lifting rod part.
  • a spring device is pretensioned.
  • the spring device moves the lifting rod up to an upper stop, the tip piston being carried along and liquid being sucked into the pipette tip.
  • the sucked-in liquid can be dispensed by pressing the control element again as far as the lower stop.
  • the user To release the pipette tip, the user must press the control element with increased force so that another spring device compresses, the upper lifting rod part is shifted downwards in the lower lifting rod part, the piston is pushed out of the lower lifting rod part and the pipette tip is pushed off the pin.
  • the spring action of the spring devices must be overcome. This can be tiring for the user, especially if the pipette tips have to be replaced frequently. Furthermore, when dispensing highly viscous liquids and when dispensing liquids rapidly from the pipette tip, the pipette tip may become detached from the pin due to the increased flow resistance in the tip opening.
  • the EP 0 992 288 B1 describes a pipette with an ejection device for a pipette tip into which a traction mechanism, pressure mechanism or articulated mechanism is integrated.
  • the ejection rod acting on the pipette tip covers a shorter distance than the drive rod connected to an ejection button, so that the ejection force is greater than the force for actuating the ejection device.
  • the ejection device is in the pipette housing on the side next to the drive device for the displacement device arranged, whereby the pipette has a large volume.
  • the pipette has the eject button in addition to the control button, so that the user has to change his grip to eject the pipette tip after pipetting.
  • the DE 103 55 914 B3 describes a pipette with an ejection device which converts an axial movement of the actuating button into an axial and rotary movement of the ejector with respect to the attachment. This is intended to reduce the effort required to operate the ejector.
  • the EP 1 689 528 B1 describes a pipette with a tip removal mechanism that includes a ramp member that is rotatable, has a ramp surface forming a circle, and includes one or more segments, the ramp surface having a high point and a low point in each segment.
  • a release element of the release device is to be forced to first move towards the tip and then to return.
  • the known ejection device is also arranged laterally next to the drive device, whereby the structural volume of the pipette is large.
  • the ejection device has a separate operating button. This is designed as an electrical button that controls a motor that rotates the ramp element via a gear. The structural effort of the discharge device is great.
  • the DE 27 11 124 C2 describes a pipette that can be connected to a pipette tip via a bayonet lock.
  • the pipette tip must be removed from the pipette by hand. This can lead to contamination.
  • the DE 10 2006 036 764 B4 describes a pipette tip which has a latching element for latching with a slip-on shaft and a sealing area for sealing on the slip-on shaft.
  • the pipette tip has a slot running through at least one slot in the axial direction of the pipette tip weakened wall. In this way, the sealing fixing of the pipette tip on the slip-on shaft and the detachment of the pipette tip from the slip-on shaft can be facilitated.
  • the pipette tip can be pushed off the shaft.
  • the US 2003/0074988 A1 describes a pipette with an ejector and an actuating device for ejecting pipette tips.
  • the actuating device has at least one cam element which is pivotably connected to the pipette housing about a horizontal hinge axis for cooperation with the ejector. This mechanism enables the cam element to have a variable torque conversion ratio when the actuating element is actuated.
  • the WO2011 / 025399 A2 describes a mechanical pipette with an ejection mechanism for pipette tips, which has a hand-operated lever that can be pivoted about a pivot axis perpendicular to the pipette axis on one side.
  • the actuating force is transmitted to the ejector by means of straight contact surfaces on the underside of the lever via an intermediate transmission means.
  • the invention is based on the object of providing a pipette which manages with a small structural volume and in which the ejection of a pipette tip requires little effort.
  • the pipette should be suitable for securely attaching a pipette tip to the peg with little effort, so that a pipette tip is prevented from being pushed off the attachment when pipetting highly viscous liquids and when pipetting quickly.
  • the cam carrier can be rotated in the pipette housing and is mounted in the pipette housing so that it cannot be displaced in the longitudinal direction of the pin.
  • the first sensing element is guided on a first curve on the circumference of the curve carrier.
  • the first scanning element is arranged on a release rod which is guided in the housing such that it can be displaced in the longitudinal direction of the pin.
  • a rotary movement of the cam carrier is converted into a linear movement of the ejector rod.
  • the rotary movement is generated by an operating element which protrudes from the pipette housing and is rotatable relative to the pipette housing and which is connected to the cam carrier.
  • the operating element protruding from the pipette housing can be operated manually by the user.
  • the ejection rod in the pipette housing can be displaced in the longitudinal direction of the pin.
  • the ejector rod pushes a pipette tip held on the pin from the pin.
  • the lower end of the ejection rod can preferably be pressed against the upper side of the pipette tip, preferably against the upper side of a piston rod of a syringe piston or against the upper side of a collar of the pipette tip.
  • the pipette tip can be ejected from the pipette by manually turning the operating element.
  • the course of the curve on the curve carrier can ensure that the force for actuating the operating element is less than the force required to eject the pipette tip.
  • a rotatably mounted curve support can be accommodated in the housing to save space.
  • the cam carrier can be designed and arranged in such a way that at least part of the drive device is arranged within the cam carrier in a space-saving manner. This allows the overall volume of the pipette to be reduced.
  • the ejection device is designed so that the cam carrier is only partially rotatable around its axis of rotation (hereinafter also referred to as "pivotable"), ie by an angle of less than 360 °, preferably less than 180 ° preferably less than 90 ° in order to push the pipette tip off the spigot.
  • the cam carrier can be rotated completely simply, several times or as often as desired about its axis of rotation in order to push the pipette tip off the spigot.
  • the pin has means for positive connection with a pipette tip, so that a pipette tip can be pushed onto the pin with elastic narrowing of the pin and / or with elastic expansion of the pipette tip before its positive connection with the pin, a locking device comprising one concentric to the pin arranged locking sleeve and a control rod protruding upward from the locking sleeve in the direction of the pin in the pipette housing, a second scanning element protrudes from the control rod and the cam support has a second curve on the circumference, on which the second scanning element is guided, which is designed in this way is that when the control element is arranged in the starting position, the locking sleeve in a locking position limits the inside of the pin and / or limits the pipette tip on the outside, whereby the locking sleeve prevents a pipette tip connected to the pin from being detached from the pin, and by rotating the operating element, the locking sleeve can be displaced upwards
  • the forces for connecting the pipette tip to the spigot and for releasing the pipette tip from the spigot can be reduced and the pipette tip can still be held sufficiently firmly on the spigot so that it is not detached from the spigot when pipetting highly viscous liquids and when pipetting quickly.
  • the connection of the pipette tip to the peg is facilitated by the fact that the peg is elastically narrowed by pushing on the pipette tip and / or the pipette tip can be elastically widened by the penetration of the peg.
  • the elastic narrowing and / or widening is brought about by the force acting between the means for positively connecting the pipette tip and the pin when it is pushed open.
  • the elastic narrowing and / or elastic widening is completely or partially reversed when the means for positively connecting the pipette tip reach the means for positively connecting the peg when it is pushed on and the pipette tip enters into the interlocking connection with the peg.
  • the at least one locking sleeve is then moved into the locking position. This secures the pipette tip on the peg, since the locking sleeve in the locked position prevents the peg from being elastically narrowed and / or the pipette tip being elastically widened, which is necessary to break the positive connection between the peg and the pipette tip.
  • the locking sleeve is removed from the locking position, so that it at least partially releases the peg and / or the pipette tip and the peg is elastically narrowed and / or the pipette tip can be elastically expanded.
  • the displacement of the locking sleeve is also controlled by the cam carrier, which has a second cam for this purpose. The shape of the second curve and its position relative to the first curve on the curve support can ensure that the locking sleeve is removed from the locking position in good time before the ejector rod presses against the pipette tip.
  • the pin can have at least one slot running in the longitudinal direction.
  • the pin can consist of a hard-elastic or soft-elastic material, for example a metal or a plastic.
  • a pin made from a soft elastic material for example from silicone rubber, a thermoplastic elastomer or rubber, can have sufficient elasticity for the elastic constriction even without a slot.
  • the pipette tip can have at least one slot running in the longitudinal direction at the upper end.
  • the pipette tip can consist of a hard-elastic plastic, for example polypropylene or polyethylene, or a soft-elastic plastic.
  • a pipette tip at least at the upper end from a soft elastic material, for example from silicone rubber, a thermoplastic elastomer or from rubber, so that it has sufficient elasticity for elastic expansion even without a slot.
  • the pipette tip can be manufactured from several materials by multi-component injection molding. The different materials can be physically and / or chemically bonded to one another. In particular, they can be positively and / or cohesively connected to one another (e.g. by gluing or welding).
  • the locking sleeve is designed in such a way that it does not deform when loaded with a radial force which is suitable for elastically narrowing the pin and / or elastically expanding the pipette tip.
  • the locking sleeve consists e.g. of a metal or a hard-elastic or rigid plastic.
  • the cam carrier is a rotary sleeve, i.e. a rotatably mounted sleeve.
  • the design as a rotating sleeve enables a particularly space-saving accommodation of at least part of the drive device within the curve support.
  • the cam carrier has an axis of rotation extending in the longitudinal direction of the pin. This is beneficial for a space-saving Accommodation of the curve support. This can for example be arranged in a space-saving manner above the drive device in the rod-shaped pipette housing.
  • the cam carrier is a rotary sleeve with an axis of rotation extending in the direction of the pin and at least part of the drive device is arranged within the rotary sleeve.
  • the cam carrier has an axis of rotation extending perpendicular to the longitudinal direction of the pin.
  • the longitudinal direction of the pin is at the same time the longitudinal direction of the rod-shaped pipette housing or essentially corresponds to it.
  • the longitudinal direction of the peg is the direction in which a pipette tip can be pushed onto the peg and detached from the peg.
  • the longitudinal direction of the pipette housing is its main direction of expansion.
  • the first curve and / or the second curve is arranged on the outer circumference of the curve carrier. This is the case, for example, when the cam carrier is designed as a solid cylinder or as a rotating sleeve. According to another embodiment, the cam carrier is a rotary sleeve and the first curve and / or the second curve is arranged on the inner circumference of the cam carrier.
  • the first curve has symmetrically arranged sections on both sides of a high point and / or the second curve on both sides of a low point in which the first and / or the second scanning element is positioned in the starting position, so that by rotating the operating element from the starting position to the pipette tip can be pushed off the spigot in different directions. This enables the user to select the direction of rotation of the operating element for ejecting the pipette tip.
  • the first curve is a first groove and / or the second curve is a second groove.
  • the first curve and / or the second curve is formed on an end face of the curve carrier, it being possible for the respective scanning elements to be pressed against the curve by a spring device.
  • the first curve has the shape of an inverted, ie upside-down, V or U or Y and / or the second curve has the shape of an upright V or U.
  • the first scanning element is a first pin, a rotatably mounted first ball, roller or sleeve or a first roller bearing mounted on a first pin and / or the second scanning element is a second pin, a rotatably mounted second ball, roller or sleeve or a second roller bearing mounted on a second journal.
  • the design as a pin is structurally particularly simple. Versions with a rotatably mounted element or a roller bearing are particularly low-friction and can reduce the effort required for actuation and wear.
  • the operating element for actuating the ejection device is at the same time an operating element for driving the drive device. This enables the pipette to be operated with one hand without changing the grip.
  • the pipette has an operating element for actuating the ejection device and an additional operating element, which is different from this operating element, for driving the drive device.
  • the operating element for driving the drive device can be displaced in the longitudinal direction of the pin.
  • the pipetting can be controlled by shifting the operating element in the longitudinal direction of the peg and the ejection of the pipette tip can be controlled by rotating the operating element about the axis of rotation.
  • control element is non-rotatably connected, i.e. fixed in rotation, to the cam carrier, so that the cam carrier exactly follows the rotation of the control element.
  • operating element is connected to the cam carrier via a gear mechanism (e.g. a gear drive or a traction mechanism).
  • the drive device has a transmission mechanism which is designed to shift a drive element of the drive device alternately downwards and upwards during successive downward displacements of the operating element, between which the operating element is displaced upward.
  • This type of embodiment is advantageous in the case of an operating element which can be displaced in the longitudinal direction of the pin in order to drive the drive device.
  • the transmission mechanism is at least partially arranged within a rotating sleeve. This enables space-saving accommodation.
  • the rotary sleeve has recesses parallel to the axis of rotation on two opposite sides
  • the operating element comprises a laterally protruding operating lever on a carrier plate
  • the carrier plate has a curved slot which is designed to receive a sector of the sleeve and along this sector to be displaced in the longitudinal direction of the pin and the carrier plate is connected to a drive of the transmission mechanism via a transmission element.
  • the operating element can be displaced within a first housing slot extending in the transverse direction of the pipette housing and within a second housing slot extending downwards from the center of the first housing slot in the longitudinal direction of the pipette housing, so that in the starting position it is at the upper end of the second housing slot in the first housing slot is pivotable in opposite directions and is linearly displaceable in the second housing slot.
  • the operating element is both non-rotatably connected to the rotary sleeve and also displaceable in the longitudinal direction of the pin. This is achieved by guiding the operating element on the curved slot on a sector of the rotating sleeve. In addition, the movements of the operating element around the axis of rotation of the rotary sleeve can be guided through the first housing slot and in the longitudinal direction of the pin through the second housing slot.
  • the rotary sleeve has a support ring at the upper end, which connects the two sectors of the sleeve to one another.
  • the sleeve is stabilized by the support ring.
  • the support ring can prevent the operating element from being pulled off the sector of the sleeve.
  • the first curve and / or the second sleeve is arranged below the cutouts on the circumference of the rotating sleeve.
  • the pipette housing and the cam carrier have a magnet arrangement and / or a spring device which is designed to automatically move the operating element into the starting position.
  • the magnet arrangement comprises, for example, two permanent magnets or a permanent magnet and a ferromagnetic component.
  • the permanent magnets or the ferromagnetic component are held on the pipette housing and on the cam carrier in such a way that they automatically move the operating element into the starting position when they come closer to one another.
  • This can also be achieved by a spring device which is pretensioned when the cam carrier is rotated out of the starting position and strives to rotate the cam carrier back into the starting position.
  • a third scanning element is provided concentrically to the pin, which is supported by a spring on the pipette housing and can be preloaded by sliding a pipette tip onto the pin, so that when a pipette tip is detached from the pin, the spring relaxes and the third scanning element releases the Pipette tip supported by the peg.
  • the third scanning element can in particular be a scanning element of a scanning device for scanning the collar of a pipette tip, as in FIG EP 18 168 763.3 described. In this respect, reference is made to the above patent application, the content of which is hereby incorporated into the above application.
  • the pipette is a positive displacement pipette or an air-cushion pipette.
  • the ejection rod When designed as a positive displacement pipette, the ejection rod is arranged within an axial bore in a lifting rod for displacing a tip piston in the pipette tip and with the lower end above a receptacle of the lifting rod for the lower end of the tip piston on the pin held and with the tip piston in a through hole of the pin engaging pipette tip and (ii.) when designed as an air-cushion pipette in the pipette housing a displacement device with a displacement element displaceable by a drive element of the drive device, which is connected to a through hole of the pin, and the lower end of the ejection rod is laterally next to the Cones arranged.
  • the ejection of a pipette tip including a tip piston is achieved by a positive displacement pipette and in the second variant (ii.) The ejection of a pipette tip is achieved by an air-cushion pipette.
  • the pipette is a single-channel pipette or a multi-channel pipette.
  • the cam carrier can control the ejector rod of a multi-channel ejector.
  • the terms “above” and “below” as well as “vertical” and “horizontal” and terms derived therefrom, such as “above” and “below”, “upright” and “upside down” and “ one above the other” refer to an arrangement of the pipette in which the pin is oriented vertically and is located at the downward-facing end of the pipette housing.
  • the pipette tip these details relate to a vertical alignment of the central axis of the pipette tip, the tip opening being arranged at the bottom and the plug-on opening being arranged at the top.
  • a pipette 1 designed as a positive displacement pipette has a rod-shaped (for example cylindrical) pipette housing 2.
  • a hollow cylindrical shaft 3 projects downward from the lower end of the pipette housing 2.
  • a pin 4 projects downward from the lower end of the shaft 3, which according to FIG Fig. 1 and 4th has a through hole 5 with a through hole at the lower end.
  • the inside diameter of the through hole 5 is smaller than the inside diameter of the shaft 3.
  • the pin 4 has an upper pin section 6 with the shape of a hollow cylinder and below it a lower pin section 7 with the shape of a hollow cone on.
  • An annular groove 8 runs around on the outer circumference of the pin 4 between the upper pin section 6 and the lower pin section 7.
  • the upper pin section 6, the annular groove 8 and the lower pin section 7 form the first means for the positive connection 9 of the pipette with a pipette tip.
  • the pin 4 has two slots 10, 11 running in its longitudinal direction, which are diametrically opposite one another.
  • the slots 10, 11 extend from the lower end over the entire length of the pin 4.
  • a drive device 12 is present in the pipette housing 2, which comprises a transmission element 13 in the form of a transmission rod 14, a transmission mechanism 15 and a drive element 16 in the form of a lifting rod 17. Furthermore, the drive device 12 comprises an operating element 18 in the form of an operating lever 19, which is firmly connected to a carrier plate 21 via a web 20.
  • the carrier plate 21 has an oval shape with a wide rounded end and a narrow rounded end, the operating lever 19 protruding from the edge of the narrow rounded end.
  • the carrier plate 21 has a first curved slot 22 which runs approximately parallel to the contour of the narrow rounded end.
  • the carrier plate 21 has a rectangular first edge recess 23 in the middle of the first curved slot 22 on the side of the narrow rounded end.
  • the lifting rod 17 is guided into the shaft 3 and the pin 4 from above.
  • it is hollow and provided with a longitudinal slot 24 starting from the lower end. Due to the longitudinal slot 24, the lifting rod 17 has a C-shaped cross section. Its lower end forms a receptacle 25 for the upper end of a piston rod.
  • the transmission mechanism 15 is designed such that when the operating lever 19 is displaced downward one after the other, between which the operating lever 19 is displaced upward, the lifting rod 17 is alternately displaced downward and upward. As a result, the lifting rod 17 can be shifted from an upper position to a lower position by pressing the operating lever 19 downward, the lifting rod 17 maintains the lower position during the subsequent upward displacement of the operating lever 19 and the lifting rod 17 is activated by a subsequent pressing of the Operating lever 19 shifted downwards again upwards. This can be repeated as often as you like.
  • the pipette 1 is provided with an ejection device 26.
  • This comprises a cam carrier 27 which is rotatably mounted in the pipette housing 2 and is designed as a hollow cylindrical rotating sleeve 28.
  • the rotating sleeve 28 is rotatably mounted, for example, with its outer circumference on the inner circumference of the pipette housing 2 and supported at the upper and lower end between shoulders of steps on the inner circumference of the pipette housing 2, so that it cannot be displaced in the axial direction in the pipette housing 2.
  • the axis of rotation of the rotary sleeve 28 coincides with the longitudinal axis of the pipette housing 2 and the longitudinal axis of the pin 4.
  • the rotating sleeve 28 has on two diametrically opposite sides to its axis of rotation parallel recesses 29, 30 which extend from the upper edge of the rotating sleeve 28 and end at a distance from its lower edge.
  • the rotary sleeve 28 thus consists of an annular base 31 and above it consists of two sectors 32, 33 of a circular ring that are diametrically opposite one another and which laterally delimit the two recesses 29, 30.
  • a first curve 34 and a second curve 35 are arranged on the outer circumference of the circular base 31 of the rotary sleeve 28.
  • the first curve 34 is designed as a first groove 36 in the form of an inverted (upside-down) Y.
  • the vertical part 37 of the Y extends far up on a sector 32 to just short of the upper edge of the sector 32.
  • the second curve 35 is a second groove 38 on the outer circumference of the base 31 of the rotating sleeve 28 with the shape of an upright standing V.
  • the first curve 34 and the second curve 35 are arranged offset from one another by 90 ° on the circumference of the rotary sleeve.
  • the first curve 34 and the second curve 35 each extend over an angular range of less than 90 ° over the circumference of the rotary sleeve 28.
  • the ejection device 26 comprises a ejection rod 39 which comprises a strip-shaped upper ejection rod part 40 and a cylindrical lower ejection rod part 41.
  • the upper and lower ejection rod parts 40, 41 are arranged parallel to one another and laterally offset from one another.
  • the lower end of the upper ejecting rod part 40 is connected to the upper end of the lower ejecting rod part 41 via a strip-shaped connecting rod part 42 inclined at an angle to the two ejecting rod parts.
  • a first scanning element 43 in the form of a first guide pin 44 projects perpendicularly from the inside of the upper ejection rod part 40.
  • the ejector rod 39 is preferably made in one piece, for example from a rigid plastic.
  • the ejection rod 39 is guided with the guide pin 44 in the first groove 36, the connecting rod part 42 extends through the longitudinal slot 24 of the lifting rod 17 and extends with the lower ejection rod part 41 within the lifting rod 17 to just before the lower end of the same.
  • the pipette 1 comprises a locking device 45 which comprises a locking sleeve 46 and a strip-shaped control rod 47 parallel thereto.
  • the upper end of the locking sleeve 46 and the lower end of the control rod 47 are via a second connecting rod part 48 connected to one another, which is inclined at an angle to the locking sleeve 46 and to the control rod 47.
  • a second scanning element 49 in the form of a second guide pin 49.1 protrudes from the inside of the control rod 47.
  • the second guide pin 49.1 is guided in the second groove 38.
  • the locking sleeve 46 is inserted into the shaft 3 from above and rests against the inside of the pin 4.
  • the lifting rod 17 and the ejection rod 39 are inserted into the locking sleeve 46 from above.
  • the operating element 19 is pushed with the first curved slot 22 onto the sector 32 of the rotary sleeve 28 on which the first groove 36 extends.
  • the rotary sleeve 28 is connected at the top to a support ring 50 which bridges the two sectors 32, 33 and stabilizes the rotary sleeve 28.
  • the support ring 50 has on the outer edge a downwardly protruding jacket 51 which laterally surrounds the two sectors 32, 33 at the outer edges. It also has a second curved slot 52 which receives the upper edge of the sector 33 which is not provided with a groove 36,38.
  • On the diametrically opposite side of the jacket 51 there is a downwardly opened rectangular second edge recess 52.1 which is designed to accommodate the web 20 between the operating lever 19 and the carrier plate 21.
  • the support ring 50 is connected to the rotating sleeve 28, for example by gluing.
  • the rotating sleeve 28 and the locking sleeve 46 as well as the operating element 18 are made, for example, of one or more rigid plastics and / or of metal.
  • the rotary sleeve 28, the support ring 50, the operating element 18 and / or the locking sleeve 46 are preferably each formed in one piece.
  • An operating button of the operating element 18 can also be made of an elastic or soft elastic plastic or rubber.
  • the operating lever 19 protrudes from the pipette housing 2 through a first housing slot running transversely to the longitudinal axis of the pipette housing 2 and extending over part of the circumference of the pipette housing 2, so that it can be operated from the outside.
  • the first housing slot is connected in the middle to a second housing slot running in the longitudinal direction of the pipette housing 2.
  • the operating lever 19 can be displaced downward from the support ring 50 along the second housing slot against the action of a spring device, sliding with the first curved slot 22 on the sector 32 of the rotating sleeve 28. After relief, the spring device automatically moves the operating lever 19 back upwards.
  • a sleeve-shaped third scanning element 53 is guided on the outside of the shaft 3.
  • a spring device in the form of a helical spring 54 guided on the shaft is supported on the underside of the pipette housing 2 and on the upper side of the third scanning element 53.
  • the sensing element 53 is pressed from above against a stop element on the shaft 3 or pin 4 by the helical spring 54.
  • the pipette housing 2 On the top of the pipette housing 2 there is an adjusting knob 55 for adjusting a dosing volume.
  • the dosing volume can be set by turning the adjusting knob 55.
  • a counter 56 arranged underneath in the pipette housing 2 shows the respectively set dosing volume.
  • the setting knob 55 and / or the counter 56 is coupled to the transmission mechanism 15.
  • the transmission mechanism 15 is designed to change the stroke of the lifting rod 17, which is carried out by shifting the operating element 18 downwards, in accordance with the metering volume set in each case.
  • a pipette tip 57 is attached to the pin 4.
  • the pipette tip 57 comprises a tubular body 58 which has a tip opening 59 at the lower end and a collar 61 having an attachment opening 60 at the upper end At the end and on the inner circumference of the collar 61 has a seat area 62 for clamping onto the pin 4.
  • the seat area 62 has a complementary contour to the pin 4, the bottom a conical lower seat section 63 for receiving the conical lower pin section 7, above a circumferential bead 64 for engaging in the annular groove 8 of the pin 4 and above a cylindrical upper seat section 65 for receiving the having cylindrical upper pin portion 6.
  • the lower seat section 63, the bead 64 and the upper seat section 65 form second means for the form-fitting connection 66 of the pipette tip 57 with the pipette 1.
  • the tubular body 58 has a cylindrical piston running area 67. Below that, the tubular body 58 has a downwardly tapering tip section 68 with the shape of a hollow truncated cone. The tip portion 68 is in Fig. 4 shown and omitted in the remaining drawings for the sake of simplicity.
  • a tip piston 69 is inserted in the tubular body 58. This comprises a piston 70 which is guided in the piston barrel region 67.
  • a piston rod 71 which has a smaller diameter than the piston 70, projects upward from the piston 70.
  • the piston rod 71 has a piston head 72 at the upper end. The piston head 72 is according to FIG Fig. 4 pressed down into the receptacle 25 of the reciprocating piston 17.
  • the pipette 1 can be used as follows: According to Fig. 1 and 8th a pipette tip 57 is held on the pipette 1 in an initial state.
  • the seat area 62 is connected to the pin 4 in a form-fitting manner, in particular by the bead 64 engaging in the annular groove 8.
  • the actuating element 18 is in the starting position at the upper end of the second housing slot, in which the first housing slot can be rotated in both directions.
  • the maximum angle of rotation is limited by the extent of the first and second grooves 36, 38 in the circumferential direction or the first housing slot, depending on which extension is smaller.
  • the locking sleeve 46 is according to Fig. 4 arranged in the lowest position, so that it prevents unintentional detachment of the pipette tip 57 from the pin 4.
  • a radial narrowing of the pin 4, which the locking sleeve 46 does not allow in this position, would namely be necessary for the release of the form-fitting connection.
  • the tip piston 69 is not yet pressed into the receptacle 25 of the lifting rod 17 with the piston head 72.
  • the pipette 1 with the lower end of the pipette tip 57 held on it is immersed in a liquid. Thereafter, the operating element 18 is pressed down again. This movement is converted by the transmission device 15 into a lifting movement of the lifting rod 17. As a result, the tip piston 69 is displaced upward.
  • the piston head 72 takes the ejector rod 39 with it, so that the first guide pin 44 slides upward in the vertical part 37 of the Y-shaped first groove 36. Meanwhile, the locking sleeve 46 maintains its position. This is in Fig. 9 shown.
  • the pipette tip 57 When the operating element 18 has carried out the set stroke, the pipette tip 57 is filled with a certain amount of liquid. Then the control element 18 relieved and shifted back upwards by the spring device until it rests on the support ring 50. In order to dispense this amount of liquid, the pipette 1 with the pipette tip 57 can be aligned with another vessel. By pressing the operating element 18 downwards again, the lifting rod 17 is shifted downwards and the amount of liquid is dispensed. Here, the first guide pin 44 slides down to the junction of the first groove 36.
  • the stroke performed by the operating element 18 when the liquid is taken up and dispensed depends on the set amount of liquid.
  • the uptake and release of liquid can be carried out several times.
  • the operating lever 18 is pivoted to the right or to the left in the starting position. This rotates the rotary sleeve 28 so that the second groove 38 displaces the second guide pin 49.1 and thus the locking sleeve 46 upwards until the locking sleeve 46 has released the pin 4 to such an extent that it can be deformed radially inward.
  • the locking sleeve 46 is preferably pulled out of the through hole 5.
  • the first guide pin 44 is displaced downward in one of the two lateral sections of the lower part of the first groove 36, so that the ejector rod 39 presses against the tip piston 69, which is supported at the bottom on the tip section 68.
  • the bead 64 exerts a radial force on the pin, so that it narrows and the positive connection between the pipette tip 57 and the pin 4 is broken.
  • the pipette tip 57 is released from the pin 4. This is in Fig. 10 shown.
  • the stripping of the pipette tip 57 from the pin 4 can also be assisted by the scanning element 53, which is pressed by the pretensioned helical spring 54 against the upper edge of the pipette tip 57.
  • a new pipette tip 57 can be connected to the pin 4.
  • the pipette 1 with the pin 4 can be inserted into the receiving opening 68 of a pipette tip 57 held ready in a carrier.
  • the scanning element 53 is displaced upwards and the helical spring 54 is pretensioned.
  • the piston head 72 presses against the underside of the ejection rod 39, so that the first guide pin 44 slides as far as the first branch point of the first groove 36.
  • the rotary sleeve 28 is rotated in the pipette housing 2 until the operating element 18 is in the starting position.
  • connection of the tip piston 69 to the lifting rod 17 and the pipetting can take place in the manner described above.
  • Fig. 1 differs from the one described above in that the locking sleeve 46 is pushed over the outer circumference of the collar 61 of the pipette tip 57 in the locking position.
  • pipette tips 57 are used which have at least one longitudinal slot extending from the upper end. The longitudinal slot enables the pipette tip 57 to be radially expanded in order to produce the form-fitting connection between the pipette tip 57 and the pin 4. The release of the positive connection is prevented when the locking sleeve 57 is in the locking position, as in FIG Fig. 11 shown.
  • the locking sleeve 46 is displaced upward by means of the rotating sleeve 28, as in the previously described embodiment, and then the pipette tip 57 is detached from the spigot 4 by pressing against the top of the tip piston 69.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Sampling And Sample Adjustment (AREA)

Claims (14)

  1. Pipette destinée à l'utilisation avec une pointe de pipette comprenant
    • un corps de pipette (2) en forme de tige,
    • un tenon (4) à l'extrémité inférieure du corps de pipette (2) servant à maintenir une pointe de pipette (57), qui présente des moyens servant à la liaison (9) par complémentarité de forme avec une pointe de pipette (57), de sorte qu'une pointe de pipette (57) peut coulisser sur le tenon (4) moyennant le rétrécissement élastique du tenon (4) et/ou moyennant l'élargissement élastique de la pointe de pipette (57) avant sa liaison par complémentarité de forme avec le tenon (4),
    • un dispositif d'entraînement (12) servant à déplacer un élément de poussée (70) pour aspirer un échantillon liquide dans une pointe de pipette (57) maintenue sur le tenon (4) et pour expulser l'échantillon hors de la pointe de pipette (57),
    • un dispositif d'éjection (18, 27, 39) comprenant un support de cames (27) monté dans le corps de pipette (2) de façon pivotante, un premier élément de palpage (43) guidé au contact d'une première came (36) sur le pourtour du support de cames (27) et adapté à une tige d'éjection (39), qui est guidée dans le sens longitudinal du tenon (4) de manière déplaçable dans le corps de pipette (2) et un élément de commande (18) relié au support de cames (27), dépassant du corps de pipette (2) et pouvant pivoter par rapport au corps de pipette (2), sachant que le dispositif d'éjection est conçu pour faite tourner le support de cames (27) par rotation de l'élément de commande (18) à partir d'une position initiale, sachant que la première came (36) déplace le premier élément de palpage (43) à tel point vers le bas que la tige d'éjection (39) chasse une pointe de pipette (57) maintenue sur le tenon (4) dudit tenon (4),
    • un dispositif de blocage (45) comprenant une douille de blocage (46) disposée concentriquement par rapport au tenon et une tige de commande (47) dépassant de la douille de blocage (46) par le haut est guidé dans le sens du tenon (4) à l'intérieur du corps de pipette (2) de façon déplaçable,
    • sachant qu'un deuxième élément de palpage (49) dépasse de la tige de commande (47) et que le support de cames (27) présente une seconde came (38) sur son pourtour, qui est conçue de manière à ce que la douille de blocage (46) limite intérieurement le tenon (4) dans une position de blocage lors de la mise en place de l'élément de commande (18) dans la position initiale et/ou limite extérieurement la pointe de pipette (57), moyennant quoi la douille de blocage (46) empêche qu'une pointe de pipette reliée au tenon (4) par complémentarité de forme ne se détache dudit tenon (4), la douille de blocage (46) peut être déplacée vers le haut par rotation de l'élément de commande (18), de sorte que le tenon (4) et/ou la pointe de pipette (57) sont au moins en partie débloqués et la tige d'éjection (39) chasse la pointe de pipette du tenon (4).
  2. Pipette selon la revendication 1, dans laquelle le support de cames (27) est une douille pivotante (28) avec un axe de rotation s'étendant dans le sens longitudinal du tenon (4) et au moins une partie du dispositif d'entrainement (12) est disposée à l'intérieur de la douille pivotante (28).
  3. Pipette selon la revendication 1 ou 2, dans laquelle la première came (36) de part et d'autre d'un point haut et/ou la seconde came de part et d'autre d'un point bas, dans lequel le premier et/ou le deuxième élément de palpage (43, 49) sont placés dans la position initiale, ont des segments disposés symétriquement, de sorte que la pointe de pipette (57) peut être chassée du tenon (4) par rotation de l'élément de commande (18) hors de la position initiale dans diverses directions.
  4. Pipette selon l'une des revendications 1 à 3, dans laquelle la première came (34) possède une première rainure (36) et/ou la seconde came (35) présente une seconde rainure (38).
  5. Pipette selon les revendications 3 et 4, dans laquelle la première came (34) présente la forme d'un V ou d'un U ou d'un Y en position retournée et/ou la seconde came (35) présente la forme d'un V ou d'un U en position droite.
  6. Pipette selon l'une des revendications 1 à 5, dans laquelle le premier élément de palpage (43) et/ou le deuxième élément de palpage (49) est un téton de guidage (44, 49.1), une bille montée de façon pivotante, un galet ou une douille ou bien un palier à roulement monté sur un tourillon.
  7. Pipette selon l'une des revendications 1 à 6, dans laquelle l'élément de commande (18) servant à actionner le dispositif d'éjection (18, 27, 39) est en même temps un élément de commande (18) servant à activer le dispositif d'entraînement (12).
  8. Pipette selon l'une des revendications 1 à 7, dans laquelle l'élément de commande (18) servant à activer le dispositif d'entraînement (12) peut être déplacé dans le sens longitudinal du tenon (4).
  9. Pipette selon l'une des revendications 1 à 8, dans laquelle le dispositif d'entraînement (12) comprend un mécanisme de transmission (15), qui est conçu pour déplacer alternativement un élément d'entraînement (13) du dispositif d'entraînement vers le bas et vers le haut lors de déplacements consécutifs de l'élément de commande (18) vers le bas, entre lesquels l'élément de commande (18) est déplacé vers le haut.
  10. Pipette selon l'une des revendications 2 à 9, dans laquelle la douille pivotante (28) présente un évidement (29, 30) parallèle à l'axe de rotation sur deux faces diamétralement opposées l'une à l'autre, l'élément de commande (18) comprend un levier de commande (19) sur une plaque de support (21), la plaque de support (21) présente une encoche incurvée (22), qui est conçue pour recevoir un secteur (32) de la douille pivotante (28) et pour être déplacée le long de ce secteur (32) dans le sens longitudinal du tenon (4), et la plaque de support (21) est reliée au mécanisme de transmission (15) par le biais d'un élément de transmission (13).
  11. Pipette selon la revendication 10, dans laquelle l'élément de commande (18) peut être déplacé à l'intérieur d'une première encoche de corps s'étendant transversalement et d'une seconde encoche de corps partant du milieu de la première encoche du corps et s'étendant dans le sens longitudinal du corps de pipette (2), de sorte qu'il est orientable dans la première encoche du corps et déplaçable dans la seconde encoche du corps de manière rectiligne.
  12. Pipette selon la revendication 10 ou 11, dans laquelle la douille pivotante (28) présente une bague d'appui (50) à son extrémité supérieure, qui relie les deux secteurs (32, 33) de la douille pivotante (28) l'un à l'autre.
  13. Pipette selon l'une des revendications 1 à 12, dans laquelle le corps de pipette (2) et le support de cames (27) comprennent un ensemble magnétique et/ou un dispositif à ressort, qui sont conçus pour déplacer automatiquement l'élément de commande (18) dans la position initiale.
  14. Pipette selon l'une des revendications 1 à 13, dans laquelle un troisième élément de palpage (53) est disponible concentriquement par rapport au tenon (4), qui prend appui sur le corps de pipette (2) par le biais d'un ressort (54) et peut être sollicité par le coulissement d'une pointe de pipette (57) sur le tenon (4), de sorte que le ressort (54) se détend lors du détachement d'une pointe de pipette (57) du tenon (4) et le troisième élément de palpage (53) favorise l'éjection de la pointe de pipette (57) du tenon (4).
EP19150808.4A 2019-01-08 2019-01-08 Pipette destinée à une utilisation avec une pointe de pipette Active EP3680016B1 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
PL19150808T PL3680016T3 (pl) 2019-01-08 2019-01-08 Pipeta do używania z końcówką
EP19150808.4A EP3680016B1 (fr) 2019-01-08 2019-01-08 Pipette destinée à une utilisation avec une pointe de pipette
JP2020001046A JP7458791B2 (ja) 2019-01-08 2020-01-07 ピペット先端部と一緒に使用するためのピペット
CN202010017457.9A CN111408423B (zh) 2019-01-08 2020-01-08 用于与移液器尖端一起使用的移液器
US16/737,483 US11426720B2 (en) 2019-01-08 2020-01-08 Pipette for use with a pipette tip

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP19150808.4A EP3680016B1 (fr) 2019-01-08 2019-01-08 Pipette destinée à une utilisation avec une pointe de pipette

Publications (2)

Publication Number Publication Date
EP3680016A1 EP3680016A1 (fr) 2020-07-15
EP3680016B1 true EP3680016B1 (fr) 2021-08-11

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EP19150808.4A Active EP3680016B1 (fr) 2019-01-08 2019-01-08 Pipette destinée à une utilisation avec une pointe de pipette

Country Status (5)

Country Link
US (1) US11426720B2 (fr)
EP (1) EP3680016B1 (fr)
JP (1) JP7458791B2 (fr)
CN (1) CN111408423B (fr)
PL (1) PL3680016T3 (fr)

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Publication number Priority date Publication date Assignee Title
EP4279182A1 (fr) 2022-05-19 2023-11-22 Eppendorf SE Pipette pour l'utilisation avec une pointe de pipette

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PL3680017T3 (pl) * 2019-01-08 2022-02-07 Eppendorf Ag Pipeta do używania z końcówką
JP7315625B2 (ja) * 2020-07-14 2023-07-26 エッペンドルフ エスイー 一体型プランジャを備えたピペット・チップとともに使用するためのピペット
USD978374S1 (en) * 2020-11-20 2023-02-14 Eppendorf Ag Pipette
GB2607893A (en) * 2021-06-11 2022-12-21 Spt Labtech Ltd Pipette tip for a liquid dispensing apparatus
FR3126891B1 (fr) * 2021-09-14 2023-09-01 Gilson Sas Dispositif de prelevement a deplacement positif, concu pour faciliter l’ejection du capillaire
CN114618602B (zh) * 2022-03-14 2024-07-26 深圳市鹰眼在线电子科技有限公司 吸头机构、移液装置及卸载和装配吸头的方法
US20230415144A1 (en) * 2022-06-28 2023-12-28 Mettler-Toledo Rainin, LLC Positive displacement pipette syringe with reduced fluid drag

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EP4279182A1 (fr) 2022-05-19 2023-11-22 Eppendorf SE Pipette pour l'utilisation avec une pointe de pipette

Also Published As

Publication number Publication date
EP3680016A1 (fr) 2020-07-15
US20200215529A1 (en) 2020-07-09
JP2020110793A (ja) 2020-07-27
JP7458791B2 (ja) 2024-04-01
PL3680016T3 (pl) 2022-04-04
CN111408423A (zh) 2020-07-14
US11426720B2 (en) 2022-08-30
CN111408423B (zh) 2022-12-27

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