EP1361283A1 - Nouveau procede de dosage - Google Patents

Nouveau procede de dosage Download PDF

Info

Publication number
EP1361283A1
EP1361283A1 EP02712305A EP02712305A EP1361283A1 EP 1361283 A1 EP1361283 A1 EP 1361283A1 EP 02712305 A EP02712305 A EP 02712305A EP 02712305 A EP02712305 A EP 02712305A EP 1361283 A1 EP1361283 A1 EP 1361283A1
Authority
EP
European Patent Office
Prior art keywords
reaction system
subject matter
measuring method
phosphogluconate dehydrogenase
phosphogluconate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP02712305A
Other languages
German (de)
English (en)
Other versions
EP1361283A4 (fr
Inventor
Koji c/o Research & Development Center KISHI
Kazuaki c/o Res. & Development Center YAMASHITA
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sysmex Corp
Original Assignee
International Reagents Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by International Reagents Corp filed Critical International Reagents Corp
Publication of EP1361283A1 publication Critical patent/EP1361283A1/fr
Publication of EP1361283A4 publication Critical patent/EP1361283A4/fr
Withdrawn legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/66—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood sugars, e.g. galactose

Definitions

  • the present invention relates to a measuring method avoiding measurement errors caused by a hemolytic constituent contained in the specimen when a subject matter is measured using a measurement reaction system which utilizes an enzyme reaction for a biological sample.
  • a method in which a subject constituent is quantitatively measured by receiving variation of amount of coenzymes (NAD, NADP, NADH, NADPH, Thio-NAD, Thio-NAD, Thio-HADH, Thio-NADP, Thio-NADPH) as variation of absorbance is often used.
  • Serum is generally used as a specimen but, since there are cases where physical impacts are given to blood cells when blood is taken to obtain serum or blood cells burst because of a human mis-operation, hemolysis may be induced.
  • hemolysis When blood is taken from a patient having a hemolytic disease, hemolysis may be recognized because of the disease.
  • measurements in which the entire blood is used to reduce the time necessary for an examination are attempted and hemolysis may also be recognized for such specimens.
  • MK myokinase
  • G6PDH glucose-6-phosphate dehydrogenase
  • a glycolysis preventive comprising a mixture of NaF, MgCl 2 , NaH 2 PO 4 and an anticoaglant in order to avoid measurement errors caused by hemolysis when a glucose constituent is measured (JP Publication of Unexamined Patent No. H05-126834) and AP 5 A is reported to be effective for MK activity.
  • the object of the invention is to provide a measuring method to obtain correct measured values when the subject matter is measured using a reaction system capable of producing 6-phosphogluconate for a biological sample.
  • 6-phosphogluconate hereinafter abbreviated to "6PG”
  • 6-PG-dehydrogenase hereinafter abbreviated to "6PGDH”
  • the present invention provides:
  • a reaction system for measuring hexose or phosphoric-acidificated hexose using coenzymes is exemplified.
  • Hexose refers to 6-carbon sugars and its representative one is glucose.
  • glucose (GLU) glucose
  • TG neutral fat
  • IP inorganicphosphorus
  • CPK creatine kinase
  • sucrose inulin
  • BUN ureanitrogen
  • creatinine are exemplified.
  • the end product in the case where a reaction system for producing glucose is used is 6PG when glucose or neutral fat is measured.
  • hemolysis interference For a reaction system for measuring hexose or phosphoric-acidificated hexose using coenzymes, there is a phenomenon that difference is produced between the correct and erred values for measured values when hemolysis occurs and this phenomenon is called hemolysis interference.
  • NAD and NADH are influenced by a dehydrogenase, especially lactate dehydrogenase (hereinafter referred to as "LDH"; NAD-depedent) in the specimen but the influence of LDH can be avoided completely by presence of oxalic acid, oxamic acid or their salts in the reaction system. Therefore, this phenomenon can be combined with the measuring method.
  • LDH lactate dehydrogenase
  • one or more enzyme ( s ) or sugar(s) selected from HK, G6PDH, hexose, G6P can be used being combined with the coenzyme.
  • Methods for measuring glucose with these enzyme approaches are widely known.
  • human red blood cells were obtained by centrifuging the blood at 3,000rpm.
  • the blood cells were frozen at -20°C and melted at a room temperature. These operations caused the blood cells to burst and hemolysis constituents were obtained.
  • the constituents were diluted with physiological salt solution to a hemoglobin concentration of 500mg/dL. Then, the reagents described as follows were prepared.
  • the following reagents were prepared and used for measurement of glucose (GLU).
  • Tris 100mM HK: 3.5U/mL
  • G6PDH 5.0U/mL
  • ⁇ -NAD 3mM magnesium acetate: 10mM pH 6.0
  • Tris 200mM ATP: 6mM pH 9.0
  • Bicine 50mM Potassium-chloride: 100mM Magnesium chloride: 10mM Nonion A-10R: 0.5% Triton X-100: 0.2% Sodium azide: 0.1% PEP: 4.0mM ATP: 3.0mM G6PDH: 4.5U/mL PK: 3.0U/mL GK: 3.0U/mL pH: 8.5
  • MES 50mM Oxalic acid: 100mM Glucose: 80mM Triton X-100: 0.25% ⁇ -NAD: 7.0mM Sodium azide: 0.1% ADP-HK: 10.0U/mL Lipase: 1500U/mL pH: 6.5
  • the operation for measurement was carried out in the same way as the measurement of glucose and neutral fat was measured.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Zoology (AREA)
  • General Physics & Mathematics (AREA)
  • Diabetes (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Genetics & Genomics (AREA)
  • Emergency Medicine (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP02712305A 2001-02-14 2002-02-13 Nouveau procede de dosage Withdrawn EP1361283A4 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2001036863 2001-02-14
JP2001036863 2001-02-14
PCT/JP2002/001173 WO2002064819A1 (fr) 2001-02-14 2002-02-13 Nouveau procede de dosage

Publications (2)

Publication Number Publication Date
EP1361283A1 true EP1361283A1 (fr) 2003-11-12
EP1361283A4 EP1361283A4 (fr) 2004-03-17

Family

ID=18900077

Family Applications (1)

Application Number Title Priority Date Filing Date
EP02712305A Withdrawn EP1361283A4 (fr) 2001-02-14 2002-02-13 Nouveau procede de dosage

Country Status (6)

Country Link
EP (1) EP1361283A4 (fr)
JP (1) JP4106270B2 (fr)
KR (1) KR100836179B1 (fr)
CN (1) CN1228636C (fr)
TW (1) TWI275795B (fr)
WO (1) WO2002064819A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1388735A1 (fr) * 2002-08-09 2004-02-11 Sysmex Corporation Reactif pour la détermination de lipides
CN105699640A (zh) * 2014-05-07 2016-06-22 北京中生金域诊断技术有限公司 一种检测肠道屏障功能的试剂盒

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100430487C (zh) * 2002-11-15 2008-11-05 江西特康科技有限公司 单一稳定烟酰胺辅酶液体试剂的制备方法
CN101324613A (zh) * 2007-06-13 2008-12-17 苏州艾杰生物科技有限公司 无机磷诊断/测定试剂盒及无机磷的浓度测定方法
WO2011136063A1 (fr) * 2010-04-30 2011-11-03 日東紡績株式会社 Procédé de mesure d'une substance spécifique, et kit de mesure d'une substance spécifique
US20160312208A1 (en) 2013-12-17 2016-10-27 Siemens Healthcare Diagnostics Inc. Preparation of multi-hapten mutant g6pdh conjugates and their use for detection of multiple analytes
CN115078341B (zh) * 2022-08-22 2022-11-29 上海执诚生物科技有限公司 一种用于检测葡萄糖-6-磷酸脱氢酶的试剂及其应用

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3732147A (en) * 1970-09-17 1973-05-08 Miles Lab Colorimetric determination of dehydrogenases
US3956069A (en) * 1974-04-29 1976-05-11 Abbott Laboratories Enzymatic assays for glucose, creatine phosphokinase or plasma ammonia
US4551427A (en) * 1983-01-31 1985-11-05 Boehringer Mannheim Gmbh Process and reagent for the determination of blood glucose in haemolysed whole blood
JPH08242892A (ja) * 1995-03-10 1996-09-24 Toyobo Co Ltd α−アミラーゼ活性測定法および測定試薬組成物
DE19756238A1 (de) * 1996-12-26 1998-07-02 Nitto Boseki Co Ltd Unterbrecher für die Konjugationsdehydrogenasereaktion, Unterbrechungsverfahren und Verfahren zur Messung einer spezifischen Substanz
US5801006A (en) * 1997-02-04 1998-09-01 Specialty Assays, Inc. Use of NADPH and NADH analogs in the measurement of enzyme activities and metabolites
US5810985A (en) * 1992-09-14 1998-09-22 Purdue Research Foundation Electrophoretically mediated chemical analysis
US6162618A (en) * 1998-04-10 2000-12-19 Smithkline Beecham Corporation 6-phosphogluconate dehydrogenase of Streptococcus pneumoniae

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1598067A1 (de) * 1951-01-28 1970-03-26 Boehringer Mannheim Gmbh Verfahren und diagnostische Mittel zur enzymatischen Bestimmung von Glucose
JPH0244520B2 (ja) * 1988-06-07 1990-10-04 Mitsubishi Petrochemical Co Gurukoosusokuteiyososeibutsu
AU7238291A (en) * 1990-02-20 1991-09-18 Iatron Laboratories, Inc. Method of determining glucose-6-phosphate and composition therefor
JPH07250698A (ja) * 1995-03-15 1995-10-03 Unitika Ltd 分析用試薬と分析方法
JP3674018B2 (ja) * 1996-12-26 2005-07-20 日東紡績株式会社 共役脱水素酵素反応の停止剤、停止方法および特定物質の測定方法

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3732147A (en) * 1970-09-17 1973-05-08 Miles Lab Colorimetric determination of dehydrogenases
US3956069A (en) * 1974-04-29 1976-05-11 Abbott Laboratories Enzymatic assays for glucose, creatine phosphokinase or plasma ammonia
US4551427A (en) * 1983-01-31 1985-11-05 Boehringer Mannheim Gmbh Process and reagent for the determination of blood glucose in haemolysed whole blood
US5810985A (en) * 1992-09-14 1998-09-22 Purdue Research Foundation Electrophoretically mediated chemical analysis
JPH08242892A (ja) * 1995-03-10 1996-09-24 Toyobo Co Ltd α−アミラーゼ活性測定法および測定試薬組成物
DE19756238A1 (de) * 1996-12-26 1998-07-02 Nitto Boseki Co Ltd Unterbrecher für die Konjugationsdehydrogenasereaktion, Unterbrechungsverfahren und Verfahren zur Messung einer spezifischen Substanz
US5801006A (en) * 1997-02-04 1998-09-01 Specialty Assays, Inc. Use of NADPH and NADH analogs in the measurement of enzyme activities and metabolites
US6162618A (en) * 1998-04-10 2000-12-19 Smithkline Beecham Corporation 6-phosphogluconate dehydrogenase of Streptococcus pneumoniae

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
PATENT ABSTRACTS OF JAPAN vol. 1997, no. 01, 31 January 1997 (1997-01-31) & JP 08 242892 A (TOYOBO CO LTD), 24 September 1996 (1996-09-24) *
RIPPA M ET AL: "6-Phosphogluconate dehydrogenase: the mechanism of action investigated by a comparison of the enzyme from different species" BIOCHIMICA ET BIOPHYSICA ACTA. PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, ELSEVIER, AMSTERDAM,, NL, vol. 1429, no. 1, 8 December 1998 (1998-12-08), pages 83-92, XP004278569 ISSN: 0167-4838 *
See also references of WO02064819A1 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1388735A1 (fr) * 2002-08-09 2004-02-11 Sysmex Corporation Reactif pour la détermination de lipides
CN105699640A (zh) * 2014-05-07 2016-06-22 北京中生金域诊断技术有限公司 一种检测肠道屏障功能的试剂盒
CN105699640B (zh) * 2014-05-07 2017-06-06 北京中生金域诊断技术股份有限公司 一种检测肠道屏障功能的试剂盒

Also Published As

Publication number Publication date
CN1228636C (zh) 2005-11-23
TWI275795B (en) 2007-03-11
JPWO2002064819A1 (ja) 2004-06-17
EP1361283A4 (fr) 2004-03-17
CN1370994A (zh) 2002-09-25
JP4106270B2 (ja) 2008-06-25
KR100836179B1 (ko) 2008-06-09
KR20020066953A (ko) 2002-08-21
WO2002064819A1 (fr) 2002-08-22

Similar Documents

Publication Publication Date Title
Zhu et al. An enzymatic colorimetric assay for glucose-6-phosphate
US4425427A (en) Simultaneous, kinetic, spectrophotometric analysis of blood serum for multiple components
CS228127B2 (en) Reagencni cinidlo
JP5906604B2 (ja) 全血検体の成分測定方法
WO1987005113A1 (fr) Regulation de reference du glucose pour bandes d'analyse du glucose
CN106868096B (zh) 一种稳定性强且成本低的己糖激酶法葡萄糖检测试剂
US6309852B1 (en) Method and reagent for quantitative determination of 1,5-anhydroglucitol
EP1361283A1 (fr) Nouveau procede de dosage
EP0639646B1 (fr) Determination tres precise d'une quantite de d-glucose-6-phosphate, et composition a cet effet
CN112159833A (zh) 一种消除内源性葡萄糖干扰的试剂及其应用和方法
US20010036636A1 (en) Biochemical method to measure niacin status in a biological sample
US7198905B2 (en) Method of screening methods prediabetic state and screening reagent
US5587296A (en) Reagent for assaying glucose
US5834227A (en) Kit for assaying creatine kinase
US7407774B2 (en) Method for reducing influence of hemoglobin with albumin in an assay
JP4544598B2 (ja) 液状試薬および保存方法
JP2000262299A (ja) グルコースデヒドロゲナーゼによるグルコースの定量方法およびグルコース定量用試薬
JP5633669B2 (ja) Ａｄｐの測定方法およびａｄｐ測定用キット
JP3078881B2 (ja) 生体試料中の成分測定法
JP4067147B2 (ja) クレアチンホスホキナーゼ活性の測定方法及び試薬
JP3034984B2 (ja) Ｄ−ガラクトースの高感度定量法および定量用組成物
EP1105524A1 (fr) Diagnostic de lesions hepatocellulaires, au moyen de fructose-1,6-biphosphatase serique
Caines et al. A fluorometric coupled enzymatic method for the determination of adenosine triphosphate in platelets
JP2001231596A (ja) 検体の溶血による影響を回避する測定方法およびその方法に使用される測定試薬
JPS60214900A (ja) 血清中総胆汁酸濃度の自動分析測定方法および測定試薬

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20030820

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR

AX Request for extension of the european patent

Extension state: AL LT LV MK RO SI

A4 Supplementary search report drawn up and despatched

Effective date: 20040203

RIC1 Information provided on ipc code assigned before grant

Ipc: 7C 12Q 1/32 A

17Q First examination report despatched

Effective date: 20050614

RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: SYSMEX CORPORATION

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20071121