EP1082749A2 - Gaseinlass für eine ionenquelle - Google Patents
Gaseinlass für eine ionenquelleInfo
- Publication number
- EP1082749A2 EP1082749A2 EP99925006A EP99925006A EP1082749A2 EP 1082749 A2 EP1082749 A2 EP 1082749A2 EP 99925006 A EP99925006 A EP 99925006A EP 99925006 A EP99925006 A EP 99925006A EP 1082749 A2 EP1082749 A2 EP 1082749A2
- Authority
- EP
- European Patent Office
- Prior art keywords
- gas
- ion source
- guide tube
- capillary
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/02—Details
- H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
- H01J49/0422—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components for gaseous samples
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/02—Details
- H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
- H01J49/0404—Capillaries used for transferring samples or ions
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/02—Details
- H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
- H01J49/0468—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components with means for heating or cooling the sample
Definitions
- the invention relates to a gas inlet for an ion source.
- the gas supply should introduce the molecules (or atoms) to be ionized into the ion source in such a way that the best possible ionization efficiency can be achieved (i.e. that a high sensitivity can be achieved in the ionization step).
- a closed one e.g. many CI or ⁇ ion sources for quadrupole or sector field mass spectrometers
- an open construction e.g. many ion sources for time of flight spectrometers [ TOF mass spectrometer]
- ion sources with a closed design an area of the ion source is "flooded" with the let-in gas, ie the let-in atoms or molecules partially collide with the ion source wall before they are ionized and detected in the mass spectrometer.
- the open design of many ion sources for TOF mass spectrometers favors the use of atomic or molecular beam technologies.
- a relatively directed gas jet is opened by the ion source, ideally it has only very little interaction with its components.
- Effective molecular beams [2], as well as skimmed [1] and unkimmed [3, 4] supersonic molecular beams (pulsed or continuous (cw)) are used for the time-of-flight mass spectrometry.
- Supersonic molecular beam emission systems allow the analysis gas to be cooled in a vacuum by adiabatic expansion.
- the expansion must take place relatively far away from the site of the ionization. Since the service of the expansion gas jet (un ⁇ ⁇ amit ⁇ ie yield for a given ionization volume) with the square of the As the expansion nozzle decreases, the sensitivity that can be achieved is limited.
- gas inlet systems for effective molecular beams can be constructed in such a way that the gas outlet is led directly to the ionization site via a metallic needle that leads m to the center of the ion source [2].
- a certain electrical potential is applied to this needle in order not to disturb the extraction fields m of the ion source.
- the needle must be heated to relatively high temperatures to prevent condensate analyte molecules from condensing out in the needle. It should be noted that the coldest point should not be at the tip of the needle. The need to heat the needle is problematic, since the needle must be electrically insulated from the rest of the structure (for example by a ceramic adapter).
- Elekt ⁇ scne insulators are generally also thermal insulators and allow only a very low heat flow of z. B. the heated lead to the needle. Heating via electrical heating elements or IR radiators is also difficult because the needle protrudes between the extraction plates of the ion source.
- the selectivity of resonance ionization with lasers depends on the inlet system used (due to the different cooling properties).
- EMB effusive molecular beam emission system
- the use of a supersonic molecular beam emission system (jet) enables ionization to be highly selective and in some cases even isomer-selective.
- the utilization of the sample quantity ie the achievable measuring sensitivity
- the existing systems are not designed to avoid memory effects.
- valves are made of inert materials are constructed to avoid memory effects or chemical decomposition (catalysis) of the sample molecules.
- the inlet valves should not have any dead volumes. It is also necessary to be able to heat the valve to temperatures of more than 200 ° C so that even non-volatile compounds from the mass range> 250 amu are accessible.
- the jet arrangement is said to lose as little sensitivity as possible to the effusive intake technology. Above all, this can be achieved by using the embedded sample more effectively compared to previous jet arrangements.
- [5] provided, for example, a heatable jet valve for analytical applications such as B. for gas chromatography-jet REMPI coupling with minimized dead volume.
- analytical applications such as B. for gas chromatography-jet REMPI coupling with minimized dead volume.
- sample utilization sensitivity
- inertness e.g. avoidance of metal-sample contact
- heatability avoidance of memory effects
- Pepich et al. presented a GC supersonic molecular beam coupling for laser-induced fluorescence spectroscopy, in which, among other things, an increase in the duty cycle compared to the effusive inlet was achieved through the pulsed inlet [6].
- the pulsed carrier gas shoots into this antechamber.
- This carrier gas compresses the analyte gas in the prechamber and, like a piston, pushes it down through a small opening into the optical chamber, where the fluorescence excitation takes place.
- the valve opening and the triggering of the laser must be synchronized in such a way that the laser beam also hits the area of the compressed analyte in the gas pulse.
- the setup thus enables a repetitive, time-limited ( ⁇ 10 ⁇ s) compression of the sample without impairing the GC flow.
- the structure of Pepich et al. no cooling of the sample (this is only achieved by inserting mixed bodies such as glass wool, which deteriorates or destroys the compression characteristics).
- the object of the invention is to design the gas inlet for an ion source so that the expansion site of the gas jet can be guided directly into the ion source of a mass spectrometer in order to achieve high sensitivity and, with the lowest possible gas load in the vacuum, the highest possible sample concentrations at the ionization site of the ion source of the mass spectrometer.
- the supersonic molecular beam expansion can be placed directly on the ion source.
- Next ⁇ back allows de device is a compression of the analyte gas in Jetgaspuls and thus a further increased sensitivity.
- Particular advantages of the gas supply are that the sample is cooled adiabatically, the capillary can be heated well up to its lower end, and the sample can be admitted in a pulsed manner.
- the device can be designed in such a way that the sample molecules only come into contact with inert materials.
- the inlet of the gas should be either pulsed or continuous. Should be further compressed by ei ⁇ NEN collision gas pressure pulse the Analytgaspulse to the Nachweisempfmd- friendliness to further increase.
- the vacuum of the mass spectrometer is created (supersonic molecular beam or jet).
- a cooling of the inlet gas is ge dieen for many mass spectrometric Fra ⁇ beneficial.
- the lower internal energy of cooled molecules often has an impact due to a reduced degree of fragmentation in the mass spectrum. Cooling is particularly advantageous for the use of resonance ionization with lasers (REMPI).
- REMPI When using a so-called supersonic molecular jet emitting system (jet) for cooling a gas jet, REMPI can be used to select highly (sometimes even isomer-selectively). Since the cooling takes place through the expansion, the sample gas supply line, the valve and the expansion nozzle can be heated without the cooling properties deteriorating significantly. This is important for analytical applications. Without sufficient heating, sample components could condense in the supply line or in the gas inlet. Important applications for the invention are the coupling of a chromatographic eluent or a continuous flow of proo gas from an on-line sampling (probe) in a supersonic molecular beam.
- the inlet system described here makes it possible to determine the expansion site m the ion source of the mass spectrometer. The ions can thus be generated directly under the expansion nozzle, which is very advantageous for the detection sensitivity that can be achieved. - -
- FIG. 1 shows a schematic gas inlet
- FIG. 2 shows the gas inlet for the ion source of a mass spectrometer
- FIG. 3 shows the compression effect
- FIG. 1 shows the diagram of an advantageous embodiment of the gas inlet, the ion source not being shown.
- the supply of the sample gas stream 13 takes place via a capillary 1 from z. B. quartz glass.
- the capillary 1 leads through a holder 7, which is made, for example, of stainless steel (mertised, Silicosteel®) or of machinable ceramic, and projects into a tube 2.
- the holder 7 is in the vacuum of the mass spectrometer. It can either be freely suspended (for example via the valve 8 and its gas supply pipe or via the heatable transfer lamp in which the capillary 1 is guided).
- the tube 2 is made chemically inert on the inside and can be made, for example, of glass, quartz or internally mertized stainless steel (silanized, Silicosteel®).
- the capillary 1 is sealed against the vacuum of the mass spectrometer by a seal 9.
- the tube 2 is attached to the bracket 7.
- the holder 7 can be heated by heating elements (not shown).
- the sample feed line (capillary 1) is guided up to the holder 7 m in a heated jacket (not shown).
- the tube 2 is also heatable.
- the tip of the tube 2 has a conductive coating, to which a defined electrical potential can be applied via a contact 14. The heating and the simultaneous application of this defined potential can, for. B.
- the heating can be carried out by fused-in micro heating wires 4.
- a metallic coating 3 is applied (for example vapor-deposited or sputtered gold layer or a very thin metal tube), to which a specific electrical potential can be applied by means of contact 14.
- the insulation of the conductive coating 3 against the housing 7 takes place, for. B. by an uncoated piece 6 of the glass tube 2nd
- a resistance heater attached to the outside of the tube 2 can be used. Many designs are conceivable. A possible embodiment of the resistance heater is set out below as an example.
- the tube 2 is provided on the outside with a metallic coating (or it is itself made of metal). In the area to be heated over this conductive coating ne e further coating is laid, the relatively high electrical resistor has Wi ⁇ (resistive coating) and with a third
- Contact coating is covered. This contact coating has no direct electrical contact with the lowest, conductive coating. If a voltage is applied between the bottom and top coating, the resistance coating acts as a resistance heater.
- an external resistor can in this case the potential of the outermost coating are selected so as You have to have a smallest possible influence on the fields in the ion source (for a given heat output).
- a resistance heater applied to the outside of the tube 2 can thus be used simultaneously for heating and applying the defined voltage.
- Another way to heat simultaneously and to place (during the Laserpulsesl the optimal potential at the outside of the coating, the On ⁇ application of pulsed heating. Just before each laser pulse, the voltage is adapted to the outer coating to the ideal value. - o -
- the end of the Rohrenen 2 has a nozzle opening 5, which can be designed in different ways.
- the nozzle 5 can be designed as a Laval nozzle.
- the tube 2 tapers towards the nozzle opening 5.
- This z. B. conical taper allows to minimize the influence of the tube 2 protruding into the ion source on the electrical extraction fields m of the ion source.
- the advantages of the gas inlet system come especially together with an advantageous embodiment of the fume hoods of the ion source z. B. a time-of-flight mass spectrometer to wear.
- the outlet characteristic from the nozzle 5 in the supersonic molecular jet mode is approximately proportional to cos 2 mich where ⁇ corresponds to the angular deviation from the straight-line gas jet [7].
- ⁇ corresponds to the angular deviation from the straight-line gas jet [7].
- the directional characteristic is less pronounced.
- the ion source should be constructed as openly as possible.
- the repeller 20 and pull-out screens 21 as the ion source as a network 17 made of thin conductive wires.
- the network 17 can, for. B. m a wire ring, a U-shaped or a rectangular holder 18 made of thicker wire.
- the upper part of the repeller plates 20 and trigger plates 21 can be solid. The ions can be withdrawn either through the network or through a circular or slot-shaped opening 22.
- the ion-optical act can be done (eg important for the achievable mass resolution).
- the design of the repeller 20 as a wire mesh 17 allows the simple use of an electron gun 23 behind the repeller 20 or in front of the trigger screen 21 to generate an electron beam for electron impact ionization (El ionization).
- the electron gun 23 can be installed at any position behind the screens (when installing behind the repeller 20 m axis with the trigger direction or different from the axis, when installing in front of the screen 21 only different from the axis).
- the electron beam 24 passes through the network 17 of the respective aperture 20 or 21 and hits the sample in the effusive molecular beam under the nozzle 5. It is advantageous that with this arrangement a time-of-flight mass spectrometer, the electron impact ionization can alternate with REMPI with a laser beam 25, ie per second Depending on the maximum repetition rate of data acquisition and processing, several hundred to thousand EI ionization mass spectra could be recorded and, in parallel, according to the maximum repetition rate of the ionization laser and the maximum repetition rate of data acquisition, a few to several ten REMPI mass spectra could be recorded.
- the device described can be operated, for example, as follows:
- valve 12 If the valve 12 is not operated, an effective molecular beam is formed under the nozzle 5 from the analyte gas stream 13 fed continuously through the capillary 1.
- the capillary 1 can be withdrawn to such an extent that it just flows into the channel 10 m of the holder 7.
- the molecules to be analyzed can be directly under the nozzle 5 z.
- REMPI laser
- EI electron beam
- the advantage of effusive operation over conventional effusive gas discharge techniques is e.g. B. the direct heatability of the part of the inlet system projecting into the ion source and the use of inert materials. If this is injected via aas valve 8 e pulse pulse gas 12 (e.g.
- argon or air with a pulse length of e.g. 750 ⁇ s forms under the nozzle 5 e supersonic molecular beam.
- the gas pulse compresses the analyte gas that has accumulated in tube 2 into a spatially restricted band.
- the analyte molecules are concentrated in the band (ie the number of analyte molecules per unit volume is increased).
- the analyte gas band represents an area with an increased analyte concentration in the jet gas pulse. This “dynamic and transient” concentration allows an improvement in the detection sensitivity.
- Figure 3 shows the compression effect recorded with a prototype of the intake system described.
- the distorted, was delay time between the laser pulse and the trigger pulse of the Ven ⁇ TILs by down in small increments 8 and registers the REMPI-Si- gnal of benzene (benzene was added to the sample gas 13).
- the length of the pulse from collision gas 12 is greater than 750 ⁇ s, the observed width of the analyte gas pulse is only 170 ⁇ s (FWHM).
- the sensitivity to the effusive inlet is significantly increased.
- the spectroscopically determined jet cooling is 15 K. This shows that very good supersonic molecular beam conditions are achieved.
- the analyte gas does not come with inner parts of e.g. B. gas valves touch, but is only performed in deactivated and inert tubes.
- the compression is done by a gas pulse.
- good jet cooling can be achieved.
- the structure described allows a sample management, as applications for trace analysis to ⁇ is required (minimized memory effects, the exclusion of catalytic reactions).
- the expansion takes place directly in the ion source of the mass spectrometer.
- the ionization site can thus be placed as close as desired to the nozzle 5 without having to use special ion-optical concepts [3] or having to drift the ions into the source.
- to avoid z. B. ion-molecular reactions and to achieve complete jet cooling e distance of 3 - 5 mm makes sense [4].
- sample gas or calibration gas can be added directly to the collision gas.
- a structure with two valves can also be implemented.
- the capillary 1 is replaced by a capillary tube 15 (not shown in the figures), into which a capillary for sample supply flows at the side and into which a compressed gas pulse can be given from above via a further valve 16 (not shown in the figures).
- the valve 8 generates a supersonic molecular jet from the nozzle opening 5 of the tube 2.
- the sample gas located in the capillary tube 15 is compressed by a further gas pulse from the valve 16, pushed out of the capillary tube 15 and injected with the already formed supersonic molecular jet of the valve 8.
- This supersonic molecular beam from valve 8 represents a so-called sheath gas flow for the sample gas pulse coming from capillary tube 15.
- the sample gas is embedded in this sheath gas and expanded through nozzle 5.
- the "jacket gas principle" permits a further increase in detection sensitivity by locally focusing the sample molecules on the central axis of the supersonic molecular beam
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Electron Tubes For Measurement (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Description
Claims
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19822674 | 1998-05-20 | ||
DE19822674A DE19822674A1 (de) | 1998-05-20 | 1998-05-20 | Gaseinlaß für eine Ionenquelle |
PCT/EP1999/003420 WO1999060603A2 (de) | 1998-05-20 | 1999-05-18 | Gaseinlass für eine ionenquelle |
Publications (2)
Publication Number | Publication Date |
---|---|
EP1082749A2 true EP1082749A2 (de) | 2001-03-14 |
EP1082749B1 EP1082749B1 (de) | 2002-04-10 |
Family
ID=7868435
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP99925006A Expired - Lifetime EP1082749B1 (de) | 1998-05-20 | 1999-05-18 | Gaseinlass für eine ionenquelle |
Country Status (7)
Country | Link |
---|---|
US (1) | US6646253B1 (de) |
EP (1) | EP1082749B1 (de) |
JP (1) | JP2002516460A (de) |
AT (1) | ATE216130T1 (de) |
DE (2) | DE19822674A1 (de) |
DK (1) | DK1082749T3 (de) |
WO (1) | WO1999060603A2 (de) |
Families Citing this family (54)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20050156991A1 (en) * | 1998-09-30 | 2005-07-21 | Optomec Design Company | Maskless direct write of copper using an annular aerosol jet |
US7938079B2 (en) * | 1998-09-30 | 2011-05-10 | Optomec Design Company | Annular aerosol jet deposition using an extended nozzle |
US7045015B2 (en) | 1998-09-30 | 2006-05-16 | Optomec Design Company | Apparatuses and method for maskless mesoscale material deposition |
US7108894B2 (en) * | 1998-09-30 | 2006-09-19 | Optomec Design Company | Direct Write™ System |
US8110247B2 (en) | 1998-09-30 | 2012-02-07 | Optomec Design Company | Laser processing for heat-sensitive mesoscale deposition of oxygen-sensitive materials |
DE19913451C2 (de) * | 1999-03-25 | 2001-11-22 | Gsf Forschungszentrum Umwelt | Gaseinlaß zur Erzeugung eines gerichteten und gekühlten Gasstrahls |
US7077960B2 (en) * | 2000-03-07 | 2006-07-18 | Solvay (Societe Anonyme) | Method for obtaining a purified hydrofluoroalkane, purified hydrofluoroalkane, use of same and method for analysing same |
FR2806077B1 (fr) | 2000-03-07 | 2004-01-30 | Solvay | Procede pour l'obtention d'un hydrofluoroalcane epure, hydrofluoroalcane epure, utilisation de l'hydrofluoroalcane et methode d'analyse d'un hydrofluoroalcane |
DE10248055B4 (de) * | 2002-10-11 | 2012-02-23 | Spectro Analytical Instruments Gmbh & Co. Kg | Methode zur Anregung optischer Atom-Emission und apparative Vorrichtung für die spektrochemische Analyse |
US10533998B2 (en) | 2008-07-18 | 2020-01-14 | Bio-Rad Laboratories, Inc. | Enzyme quantification |
GB0307403D0 (en) | 2003-03-31 | 2003-05-07 | Medical Res Council | Selection by compartmentalised screening |
GB0307428D0 (en) | 2003-03-31 | 2003-05-07 | Medical Res Council | Compartmentalised combinatorial chemistry |
US20060078893A1 (en) | 2004-10-12 | 2006-04-13 | Medical Research Council | Compartmentalised combinatorial chemistry by microfluidic control |
US20050221339A1 (en) | 2004-03-31 | 2005-10-06 | Medical Research Council Harvard University | Compartmentalised screening by microfluidic control |
US7968287B2 (en) | 2004-10-08 | 2011-06-28 | Medical Research Council Harvard University | In vitro evolution in microfluidic systems |
US20060280866A1 (en) * | 2004-10-13 | 2006-12-14 | Optomec Design Company | Method and apparatus for mesoscale deposition of biological materials and biomaterials |
US7674671B2 (en) | 2004-12-13 | 2010-03-09 | Optomec Design Company | Aerodynamic jetting of aerosolized fluids for fabrication of passive structures |
US7938341B2 (en) | 2004-12-13 | 2011-05-10 | Optomec Design Company | Miniature aerosol jet and aerosol jet array |
DE102005005333B4 (de) * | 2005-01-28 | 2008-07-31 | Deutsches Zentrum für Luft- und Raumfahrt e.V. | Verfahren zur Probennahme und Aerosol-Analyse |
DE102005032983B4 (de) * | 2005-07-14 | 2007-05-31 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Düsenanordnung |
EP1984738A2 (de) | 2006-01-11 | 2008-10-29 | Raindance Technologies, Inc. | Mikrofluidische vorrichtungen und verfahren zur verwendung bei der bildung und kontrolle von nanoreaktoren |
US7855357B2 (en) * | 2006-01-17 | 2010-12-21 | Agilent Technologies, Inc. | Apparatus and method for ion calibrant introduction |
EP2530167A1 (de) | 2006-05-11 | 2012-12-05 | Raindance Technologies, Inc. | Mikrofluidische Vorrichtungen |
US9562837B2 (en) | 2006-05-11 | 2017-02-07 | Raindance Technologies, Inc. | Systems for handling microfludic droplets |
WO2008021123A1 (en) | 2006-08-07 | 2008-02-21 | President And Fellows Of Harvard College | Fluorocarbon emulsion stabilizing surfactants |
WO2008097559A2 (en) | 2007-02-06 | 2008-08-14 | Brandeis University | Manipulation of fluids and reactions in microfluidic systems |
US8592221B2 (en) | 2007-04-19 | 2013-11-26 | Brandeis University | Manipulation of fluids, fluid components and reactions in microfluidic systems |
TWI482662B (zh) | 2007-08-30 | 2015-05-01 | Optomec Inc | 機械上一體式及緊密式耦合之列印頭以及噴霧源 |
TWI538737B (zh) | 2007-08-31 | 2016-06-21 | 阿普托麥克股份有限公司 | 材料沉積總成 |
US8887658B2 (en) | 2007-10-09 | 2014-11-18 | Optomec, Inc. | Multiple sheath multiple capillary aerosol jet |
EP2315629B1 (de) | 2008-07-18 | 2021-12-15 | Bio-Rad Laboratories, Inc. | Tröpfchenbibliotheken |
US8528589B2 (en) | 2009-03-23 | 2013-09-10 | Raindance Technologies, Inc. | Manipulation of microfluidic droplets |
EP2438605A4 (de) * | 2009-06-03 | 2016-09-28 | Univ Wayne State | Massenspektrometrie mithilfe von laserspray-ionisierung |
US10520500B2 (en) | 2009-10-09 | 2019-12-31 | Abdeslam El Harrak | Labelled silica-based nanomaterial with enhanced properties and uses thereof |
WO2011079176A2 (en) | 2009-12-23 | 2011-06-30 | Raindance Technologies, Inc. | Microfluidic systems and methods for reducing the exchange of molecules between droplets |
US9399797B2 (en) | 2010-02-12 | 2016-07-26 | Raindance Technologies, Inc. | Digital analyte analysis |
EP2534267B1 (de) | 2010-02-12 | 2018-04-11 | Raindance Technologies, Inc. | Digitale analyse von analyten |
US10351905B2 (en) | 2010-02-12 | 2019-07-16 | Bio-Rad Laboratories, Inc. | Digital analyte analysis |
US9366632B2 (en) | 2010-02-12 | 2016-06-14 | Raindance Technologies, Inc. | Digital analyte analysis |
EP3447155A1 (de) | 2010-09-30 | 2019-02-27 | Raindance Technologies, Inc. | Sandwichassays in tröpfchen |
EP3859011A1 (de) | 2011-02-11 | 2021-08-04 | Bio-Rad Laboratories, Inc. | Verfahren zur bildung gemischter tröpfchen |
US9150852B2 (en) | 2011-02-18 | 2015-10-06 | Raindance Technologies, Inc. | Compositions and methods for molecular labeling |
US8841071B2 (en) | 2011-06-02 | 2014-09-23 | Raindance Technologies, Inc. | Sample multiplexing |
US9945812B2 (en) * | 2011-07-15 | 2018-04-17 | Wayne State University | Simultaneous ion sensing and gas sampling in combustion engine cylinders and other combustion systems |
US8658430B2 (en) | 2011-07-20 | 2014-02-25 | Raindance Technologies, Inc. | Manipulating droplet size |
EP2669929A1 (de) * | 2012-05-29 | 2013-12-04 | Technische Universität München | Hochleistungs-Ionenquelle und Verfahren zum Erzeugen eines Ionenstrahls |
US11901041B2 (en) | 2013-10-04 | 2024-02-13 | Bio-Rad Laboratories, Inc. | Digital analysis of nucleic acid modification |
US9944977B2 (en) | 2013-12-12 | 2018-04-17 | Raindance Technologies, Inc. | Distinguishing rare variations in a nucleic acid sequence from a sample |
EP3090063B1 (de) | 2013-12-31 | 2019-11-06 | Bio-Rad Laboratories, Inc. | Verfahren zur detektion von latentem retrovirus |
CN107548346B (zh) | 2015-02-10 | 2021-01-05 | 奥普托美克公司 | 通过气溶胶的飞行中固化制造三维结构 |
US10647981B1 (en) | 2015-09-08 | 2020-05-12 | Bio-Rad Laboratories, Inc. | Nucleic acid library generation methods and compositions |
CN111655382B (zh) | 2017-11-13 | 2022-05-31 | 奥普托美克公司 | 气溶胶流的阻挡 |
DE102018216623A1 (de) * | 2018-09-27 | 2020-04-02 | Carl Zeiss Smt Gmbh | Massenspektrometer und Verfahren zur massenspektrometrischen Analyse eines Gases |
CN113484401B (zh) * | 2021-07-07 | 2022-01-11 | 中国科学院地质与地球物理研究所 | 一种在轨标校质谱仪基础参数的方法 |
Family Cites Families (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE2942386C2 (de) * | 1979-10-19 | 1984-01-12 | Ulrich Dr. 8000 München Boesl | Ionenquelle |
DE3424696A1 (de) * | 1984-07-05 | 1986-02-06 | Ringsdorff-Werke GmbH, 5300 Bonn | Vorrichtung zur zufuehrung von analysensubstanz in ein plasma |
JP2765890B2 (ja) * | 1988-12-09 | 1998-06-18 | 株式会社日立製作所 | プラズマイオン源微量元素質量分析装置 |
US5070240B1 (en) * | 1990-08-29 | 1996-09-10 | Univ Brigham Young | Apparatus and methods for trace component analysis |
DE4108462C2 (de) * | 1991-03-13 | 1994-10-13 | Bruker Franzen Analytik Gmbh | Verfahren und Vorrichtung zum Erzeugen von Ionen aus thermisch instabilen, nichtflüchtigen großen Molekülen |
US6011259A (en) * | 1995-08-10 | 2000-01-04 | Analytica Of Branford, Inc. | Multipole ion guide ion trap mass spectrometry with MS/MSN analysis |
DE4441972C2 (de) * | 1994-11-25 | 1996-12-05 | Deutsche Forsch Luft Raumfahrt | Verfahren und Vorrichtung zum Nachweis von Probenmolekülen in einem Trägergas |
DE19539589C2 (de) * | 1995-10-25 | 1999-01-28 | Boesl Ulrich Priv Doz Dr | Gepulstes Ventil für die Kopplung einer Gaschromatographie-Kapillare an ein sekundäres Spurenanalysegerät mittels eines Überschallstrahles |
US5868322A (en) * | 1996-01-31 | 1999-02-09 | Hewlett-Packard Company | Apparatus for forming liquid droplets having a mechanically fixed inner microtube |
US5788166A (en) * | 1996-08-27 | 1998-08-04 | Cornell Research Foundation, Inc. | Electrospray ionization source and method of using the same |
WO1998009316A1 (fr) * | 1996-08-29 | 1998-03-05 | Nkk Corporation | Spectroscope de masse a ionisation par laser et procede d'analyse par spectroscopie de masse |
US5742050A (en) * | 1996-09-30 | 1998-04-21 | Aviv Amirav | Method and apparatus for sample introduction into a mass spectrometer for improving a sample analysis |
EP1021819B1 (de) * | 1997-09-12 | 2005-03-16 | Analytica Of Branford, Inc. | Mehrprobeneinführungs-massenspektrometrie |
WO1999041585A2 (en) * | 1998-02-13 | 1999-08-19 | Tsi Incorporated | Instrument for measuring and classifying nanometer aerosols |
US6348687B1 (en) * | 1999-09-10 | 2002-02-19 | Sandia Corporation | Aerodynamic beam generator for large particles |
-
1998
- 1998-05-20 DE DE19822674A patent/DE19822674A1/de not_active Ceased
-
1999
- 1999-05-18 JP JP2000550131A patent/JP2002516460A/ja active Pending
- 1999-05-18 DK DK99925006T patent/DK1082749T3/da active
- 1999-05-18 AT AT99925006T patent/ATE216130T1/de not_active IP Right Cessation
- 1999-05-18 DE DE59901196T patent/DE59901196D1/de not_active Expired - Fee Related
- 1999-05-18 EP EP99925006A patent/EP1082749B1/de not_active Expired - Lifetime
- 1999-05-18 WO PCT/EP1999/003420 patent/WO1999060603A2/de active IP Right Grant
-
2000
- 2000-11-17 US US09/718,472 patent/US6646253B1/en not_active Expired - Fee Related
Non-Patent Citations (1)
Title |
---|
See references of WO9960603A2 * |
Also Published As
Publication number | Publication date |
---|---|
JP2002516460A (ja) | 2002-06-04 |
DK1082749T3 (da) | 2002-07-22 |
WO1999060603A2 (de) | 1999-11-25 |
US6646253B1 (en) | 2003-11-11 |
DE59901196D1 (de) | 2002-05-16 |
EP1082749B1 (de) | 2002-04-10 |
DE19822674A1 (de) | 1999-12-09 |
WO1999060603A3 (de) | 2000-01-27 |
ATE216130T1 (de) | 2002-04-15 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP1082749B1 (de) | Gaseinlass für eine ionenquelle | |
EP1166328B1 (de) | Gaseinlass zur erzeugung eines gerichteten und gekühlten gasstrahls | |
US6649907B2 (en) | Charge reduction electrospray ionization ion source | |
Olivares et al. | Ion sampling for inductively coupled plasma mass spectrometry | |
US6974957B2 (en) | Ionization device for aerosol mass spectrometer and method of ionization | |
DE69936168T2 (de) | Mehrfachprobeninlassmassenspektrometer | |
US4999493A (en) | Electrospray ionization interface and method for mass spectrometry | |
US8003934B2 (en) | Methods and apparatus for ion sources, ion control and ion measurement for macromolecules | |
DE3913763C2 (de) | Massenspektrometer | |
AU777133B2 (en) | Parallel sample introduction electrospray mass spectrometer with electronic indexing through multiple ion entrance orifices | |
AT405472B (de) | Verfahren und vorrichtung zum erzeugen eines plasmas | |
DE4303027A1 (de) | ||
EP3491659B1 (de) | Niedertemperatur-plasmasonde mit zusätzlichem erhitztem gasstrahl | |
DE3938314C2 (de) | Massenspektrometer | |
WO2018229724A2 (de) | Vorrichtung und verfahren zur ionisation eines analyten sowie vorrichtung und verfahren zur analyse eines ionisierten analyten | |
DE112019002405T5 (de) | Zweistufige Ionenquelle, geschlossene und offene Ionenvolumen aufweisend | |
DE102005004804B4 (de) | Ionenquelle und Verfahren zur Ionisierung einer Probe | |
EP1269051A1 (de) | Pulsbares kapillarventil | |
US8835838B2 (en) | Method and apparatus for analysis and ion source | |
DE102004033993B4 (de) | Ionenquelle für ein Massenspektrometer | |
JP2021514109A (ja) | 統合型エレクトロスプレーイオン源 | |
DE102016113771B4 (de) | Analysevorrichtung für gasförmige Proben und Verfahren zum Nachweis von Analyten in einem Gas | |
Hattendorf | Ion molecule reactions for the suppression of spectral interferences in elemental analysis by inductively coupled plasma mass spectrometry | |
DE10259831B4 (de) | Plasmagenerator | |
KR20230014074A (ko) | 액체 크로마토그래피, 이온화 장치 그리고 질량 분석기의 인터페이스 및 이를 이용한 시료 분석 방법 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
17P | Request for examination filed |
Effective date: 20000816 |
|
AK | Designated contracting states |
Kind code of ref document: A2 Designated state(s): AT BE CH DE DK FI FR GB IE IT LI NL SE |
|
GRAG | Despatch of communication of intention to grant |
Free format text: ORIGINAL CODE: EPIDOS AGRA |
|
17Q | First examination report despatched |
Effective date: 20010629 |
|
GRAG | Despatch of communication of intention to grant |
Free format text: ORIGINAL CODE: EPIDOS AGRA |
|
GRAH | Despatch of communication of intention to grant a patent |
Free format text: ORIGINAL CODE: EPIDOS IGRA |
|
GRAH | Despatch of communication of intention to grant a patent |
Free format text: ORIGINAL CODE: EPIDOS IGRA |
|
REG | Reference to a national code |
Ref country code: GB Ref legal event code: IF02 |
|
GRAA | (expected) grant |
Free format text: ORIGINAL CODE: 0009210 |
|
AK | Designated contracting states |
Kind code of ref document: B1 Designated state(s): AT BE CH DE DK FI FR GB IE IT LI NL SE |
|
REF | Corresponds to: |
Ref document number: 216130 Country of ref document: AT Date of ref document: 20020415 Kind code of ref document: T |
|
REG | Reference to a national code |
Ref country code: CH Ref legal event code: EP |
|
REG | Reference to a national code |
Ref country code: CH Ref legal event code: NV Representative=s name: ROTTMANN, ZIMMERMANN + PARTNER AG Ref country code: IE Ref legal event code: FG4D Free format text: GERMAN |
|
REF | Corresponds to: |
Ref document number: 59901196 Country of ref document: DE Date of ref document: 20020516 |
|
REG | Reference to a national code |
Ref country code: DK Ref legal event code: T3 |
|
GBT | Gb: translation of ep patent filed (gb section 77(6)(a)/1977) |
Effective date: 20020710 |
|
ET | Fr: translation filed | ||
PLBE | No opposition filed within time limit |
Free format text: ORIGINAL CODE: 0009261 |
|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: NO OPPOSITION FILED WITHIN TIME LIMIT |
|
26N | No opposition filed |
Effective date: 20030113 |
|
PGFP | Annual fee paid to national office [announced via postgrant information from national office to epo] |
Ref country code: BE Payment date: 20030428 Year of fee payment: 5 |
|
PGFP | Annual fee paid to national office [announced via postgrant information from national office to epo] |
Ref country code: CH Payment date: 20030429 Year of fee payment: 5 |
|
PGFP | Annual fee paid to national office [announced via postgrant information from national office to epo] |
Ref country code: DK Payment date: 20030514 Year of fee payment: 5 |
|
PGFP | Annual fee paid to national office [announced via postgrant information from national office to epo] |
Ref country code: SE Payment date: 20030519 Year of fee payment: 5 |
|
PGFP | Annual fee paid to national office [announced via postgrant information from national office to epo] |
Ref country code: AT Payment date: 20030526 Year of fee payment: 5 |
|
PGFP | Annual fee paid to national office [announced via postgrant information from national office to epo] |
Ref country code: NL Payment date: 20030528 Year of fee payment: 5 Ref country code: FR Payment date: 20030528 Year of fee payment: 5 |
|
PGFP | Annual fee paid to national office [announced via postgrant information from national office to epo] |
Ref country code: GB Payment date: 20040308 Year of fee payment: 6 |
|
PGFP | Annual fee paid to national office [announced via postgrant information from national office to epo] |
Ref country code: IE Payment date: 20040504 Year of fee payment: 6 Ref country code: FI Payment date: 20040504 Year of fee payment: 6 |
|
PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: AT Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 20040518 |
|
PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: SE Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 20040519 |
|
PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: LI Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 20040531 Ref country code: CH Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 20040531 Ref country code: BE Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 20040531 |
|
PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: DK Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 20040601 |
|
BERE | Be: lapsed |
Owner name: *GSF-FORSCHUNGSZENTRUM FUR UMWELT UND GESUNDHEIT G Effective date: 20040531 |
|
PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: NL Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 20041201 |
|
EUG | Se: european patent has lapsed | ||
REG | Reference to a national code |
Ref country code: DK Ref legal event code: EBP |
|
REG | Reference to a national code |
Ref country code: CH Ref legal event code: PL |
|
PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: FR Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 20050131 |
|
NLV4 | Nl: lapsed or anulled due to non-payment of the annual fee |
Effective date: 20041201 |
|
REG | Reference to a national code |
Ref country code: FR Ref legal event code: ST |
|
PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: FI Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 20050506 |
|
PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: IT Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 20050518 Ref country code: GB Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 20050518 |
|
GBPC | Gb: european patent ceased through non-payment of renewal fee |
Effective date: 20050518 |
|
REG | Reference to a national code |
Ref country code: IE Ref legal event code: MM4A |
|
PGFP | Annual fee paid to national office [announced via postgrant information from national office to epo] |
Ref country code: DE Payment date: 20060309 Year of fee payment: 8 |
|
PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: DE Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 20071201 |