EP0654527B1 - Verfahren zum Entschleimen von Pflanzenöl mittels Enzymen - Google Patents

Verfahren zum Entschleimen von Pflanzenöl mittels Enzymen Download PDF

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Publication number
EP0654527B1
EP0654527B1 EP94203211A EP94203211A EP0654527B1 EP 0654527 B1 EP0654527 B1 EP 0654527B1 EP 94203211 A EP94203211 A EP 94203211A EP 94203211 A EP94203211 A EP 94203211A EP 0654527 B1 EP0654527 B1 EP 0654527B1
Authority
EP
European Patent Office
Prior art keywords
oil
enzymes
degumming
sludge
degummed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
EP94203211A
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German (de)
English (en)
French (fr)
Other versions
EP0654527A1 (de
Inventor
Henning Dr. Buchold
Rudolf Dr. Boensch
Joerg Dr. Schroeppel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GEA Group AG
Roehm GmbH Darmstadt
Original Assignee
Metallgesellschaft AG
Roehm GmbH Darmstadt
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Metallgesellschaft AG, Roehm GmbH Darmstadt filed Critical Metallgesellschaft AG
Publication of EP0654527A1 publication Critical patent/EP0654527A1/de
Application granted granted Critical
Publication of EP0654527B1 publication Critical patent/EP0654527B1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00—Refining fats or fatty oils
    • C11B3/003—Refining fats or fatty oils by enzymes or microorganisms, living or dead

Definitions

  • the invention relates to a method for degumming Vegetable oil, whereby the vegetable oil is adjusted to a pH of 3 to 6, an aqueous enzyme solution dispersed in the oil, which contains one of the enzymes phospholipase A1, A2 or B, which Enzymes in the oil with stirring at temperatures from 20 to 90 ° C in a degumming reactor can act and from the Degumming reactor withdrawn liquid degummed oil separates.
  • the invention is based, at the beginning mentioned processes the used enzymes in Degumming process at least partially to reuse. According to the invention, this is done in that the liquid withdrawn from the degumming reactor at temperatures of 20 to 90 ° C before or after the separation of the degummed oil a release agent or a Solubilizer admits and an aqueous, used enzyme containing, largely sludge-free solution that one at least partially leads back to the degumming reactor and dispersed in the oil to be degummed, the proportion used, recycled enzymes in total the enzyme dispersed in the oil is at least 10%. For cost reasons, it is advantageous if the share used, recycled enzymes in total of the enzymes dispersed in the oil at least 20% or more better, at least 50%.
  • a variant of the 2nd way is that of the Degumming reactor coming oily liquid admit a broker and then that separate degummed oil from the liquid, e.g. in a centrifuge. You get next to that degummed oil an aqueous, largely sludge-free Phase that contains the used enzymes. This The aqueous phase can be wholly or partly without further Treatment, before returning the degumming reactor, to mix them with the oil to be degummed.
  • solubilizer to be used or Release aids can vary widely, they is usually 0.1 to 100 g / l liquid. A Excess solubilizer or separation aid is not disturbing.
  • the vegetable oil to be completely degummed comes in accordance with Fig. 1 from the line (1), it is dosed from the Storage container (2) first an aqueous acidic solution, e.g. Citric acid, and one from the storage container (3) aqueous alkaline solution, e.g. Caustic soda, in such Amounts that the oil has a pH of 3 to 6 preferably about 5, through line (4) into one first disperser (5) occurs. In the disperser (5) an oil-water emulsion is created, which can be Line (6) in a retention tank (7) passes.
  • aqueous acidic solution e.g. Citric acid
  • aqueous alkaline solution e.g. Caustic soda
  • the reactor (13) shown has three levels (13a), (13b) and (13c), through which the oil to be treated with a certain dwell time on each floor from top to bottom flows.
  • Each floor can have different Treatment temperatures can be set, it is recommends on the top floor (13a) with the lowest temperature and on the lowest floor (13c) to work with the highest temperature.
  • the Treatment temperatures in the reactor (13) are in the range from 20 to 90 ° C with a total residence time in Reactor (13) of usually 3 to 5 hours.
  • the treated oil leaves the degumming reactor (13) through line (17) and becomes a centrifuge (18) given up.
  • the centrifuge is used as a product degummed oil obtained in the line (19) dissipates.
  • the enzyme-containing aqueous sludge phase in the Line (20) is given from the storage container (21) Separation aid and stir the whole thing in Disperser (22) intensive. Then you give the stirred phase in line (23) one Indwelling container (24), preferably with a Stirring device (25) is provided.
  • the temperatures are in the range from 30 to 85 ° C and preferably at about 60 ° C and man ensures dwell times in the range of 3 to 60 minutes.
  • 2 is correct between the Lines (1) and (17) with the previously together with Fig. 1st described method. 2 is also that coming from the degumming reactor (13) Water-phosphatide sludge-oil mixture through the Line (17) fed to the centrifuge (18), from which one withdraws degummed oil in line (19). Of the enzyme-containing water-sludge phase of the line (30) there one from the reservoir (31) solubilizer to and stir the whole thing in the retention tank (32). Here creates an aqueous, enzyme-containing solution, which one by the line (28) in the reservoir (10) to Reuse returns; an excess of the solution can be removed in line (29). In place the retention tank (32) can also be a Disperser (22), cf. Fig. 1.
  • rapeseed oil which with Water has been degummed in various ways Enzymes degummed.
  • Examples 1, 5 and 6 are Comparative examples in which the invention Procedure is not applied.
  • the degummed rapeseed oil in line (19) has one remaining phosphorus content of 6 ppm, the volume of Water-sludge phase is 370 ml.
  • the phosphorus content is a measure of the level of degumming, good degummed oil has a residual P content of less than 10 ppm.
  • Example 1 For the procedure according to the invention according to FIG. 2 Example 1 is repeated, with the Water-sludge phase of line (30) 3 g / l TWEEN 80 adds and the liquid 10 min in a disperser (Ultra-Turrax) stirs intensely. You get one sludge-free, enzyme-containing solution with no suspended Particles that one in the line (28) in the Returns storage container (10). On adding fresher Enzymes are avoided. The degummed oil of the Line (19) has a residual phosphorus content of 5 ppm per run.
  • a disperser Ultra-Turrax
  • Example 1 For the procedure according to the invention according to FIG. 2 Example 1 is repeated, the one from the Enzyme reactor exiting via line 17 Liquid mixture via line (33) 2.5 g Tween 80 per liter of mixture and the mixture in the Centrifuge 18 separates. You largely get one sludge-free, enzyme-containing solution that can be piped (34) and line (28) to the reservoir (10). Fresh enzymes are not added.
  • the degummed oil in line (19) has one Residual phosphorus content of 5 ppm.
  • Example 1 For the procedure according to the invention according to FIG. 1 Example 1 is repeated, with the Water-sludge phase of the line (20) per liter 2 g TWEEN 80 (manufacturer DuPont) adds and the Liquids in a disperser (22) (Ultra-Turrax) homogenized. After a dwell time of 10 minutes the phosphatide sludge by centrifuging the aqueous enzyme solution separated. The enzyme solution will via the line (28) into the storage container (10) traced back, adding fresher addition No enzyme solution whatsoever. The residual phosphorus content in the degummed oil of line (19) is 5 ppm.
  • Example 4 is modified so that instead of aqueous enzyme solution after centrifugation in the Line (27) occurring, pretreated with TWEEN 80 Phosphatide sludge via the dosing point (9) with the oil the line (8) is mixed. It turns out that this Phosphatide sludge to improve degumming is ineffective because it has no enzyme activity.
  • Example 1 For the procedure according to the invention Example 1 is repeated, taking the water-sludge phase the line (20) 2 liters of alginate protan per liter (Manufacturer: Pronova-Biopolymer, Norway) adds and the liquids in a disperser (22) (Ultra-Turrax) homogenized, cf. Fig. 1. After a The phosphatide sludge becomes a residence time of 10 minutes by centrifuging the aqueous enzyme solution severed. The enzyme solution is in the Storage container (10) returned. Without adding fresh enzymes the residual phosphorus content in degummed oil of line (19) 8 ppm.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Microbiology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Fats And Perfumes (AREA)
  • Edible Oils And Fats (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Treatment Of Sludge (AREA)
EP94203211A 1993-11-19 1994-11-04 Verfahren zum Entschleimen von Pflanzenöl mittels Enzymen Expired - Lifetime EP0654527B1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE4339556 1993-11-19
DE4339556A DE4339556C1 (de) 1993-11-19 1993-11-19 Verfahren zum Entschleimen von Pflanzenöl mittels Enzymen

Publications (2)

Publication Number Publication Date
EP0654527A1 EP0654527A1 (de) 1995-05-24
EP0654527B1 true EP0654527B1 (de) 1998-01-14

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EP94203211A Expired - Lifetime EP0654527B1 (de) 1993-11-19 1994-11-04 Verfahren zum Entschleimen von Pflanzenöl mittels Enzymen

Country Status (12)

Country Link
US (1) US5558781A (zh)
EP (1) EP0654527B1 (zh)
JP (1) JPH07188691A (zh)
CN (1) CN1046760C (zh)
AT (1) ATE162210T1 (zh)
BR (1) BR9404496A (zh)
CA (1) CA2136050A1 (zh)
DE (2) DE4339556C1 (zh)
DK (1) DK0654527T3 (zh)
ES (1) ES2111841T3 (zh)
GR (1) GR3026501T3 (zh)
TW (1) TW279900B (zh)

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US7666618B2 (en) 2004-07-16 2010-02-23 Danisco A/S Lipolytic enzyme: uses thereof in the food industry
US7718408B2 (en) 2003-12-24 2010-05-18 Danisco A/S Method
US7718204B2 (en) 1998-07-21 2010-05-18 Danisco A/S Foodstuff
US7807398B2 (en) 2003-01-17 2010-10-05 Danisco A/S Method of using lipid acyltransferase
US7906307B2 (en) 2003-12-24 2011-03-15 Danisco A/S Variant lipid acyltransferases and methods of making
US7955814B2 (en) 2003-01-17 2011-06-07 Danisco A/S Method
US7960150B2 (en) 2007-01-25 2011-06-14 Danisco A/S Production of a lipid acyltransferase from transformed Bacillus licheniformis cells
US8012732B2 (en) 2004-03-12 2011-09-06 Danisco A/S Fungal lypolytic and amylase enzyme composition and methods using the same
US8030044B2 (en) 2003-12-24 2011-10-04 Danisco A/S Lipid acyltransferases
USRE43135E1 (en) 2001-05-18 2012-01-24 Danisco A/S Method of improving dough and bread quality
USRE43341E1 (en) 1995-06-07 2012-05-01 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
US8652809B2 (en) 2007-08-17 2014-02-18 Dupont Nutrition Biosciences Aps Method for producing ultra-heat treatment milk

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US6406723B1 (en) * 1997-04-09 2002-06-18 Danisco A/S Method for preparing flour doughs and products made from such doughs using glycerol oxidase and lipase
US6103505A (en) * 1996-12-09 2000-08-15 Novo Nordisk A/S Method for reducing phosphorus content of edible oils
WO1998026057A1 (en) * 1996-12-09 1998-06-18 Novo Nordisk A/S Reduction of phosphorus containing components in edible oils comprising a high amount of non-hydratable phosphorus by use of a phospholipase, a phospholipase from a filamentous fungus having phospholipase a and/or b activity
EP1071734A1 (en) * 1998-04-08 2001-01-31 Novozymes A/S An enzymatic oil-degumming process
TR200101497T2 (tr) 1998-11-27 2001-11-21 Novozymes A/S Lipolitik enzim varyantları
US6166231A (en) * 1998-12-15 2000-12-26 Martek Biosciences Corporation Two phase extraction of oil from biomass
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US7226771B2 (en) 2002-04-19 2007-06-05 Diversa Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
ES2395898T3 (es) 2002-04-19 2013-02-15 Dsm Ip Assets B.V. Fosfolipasas, ácidos nucleicos que las codifican, y métodos para obtenerlas y usarlas
CN100545261C (zh) 2002-05-21 2009-09-30 Dsmip资产有限公司 新的磷脂酶及其用途
CA3007908A1 (en) 2003-03-07 2005-04-14 Dsm Ip Assets B.V. Hydrolases, nucleic acids encoding them and methods for making and using them
EP2468853B1 (en) 2004-06-16 2015-04-01 DSM IP Assets B.V. Method for enzymatic decolorization of pheophytin
US20080070291A1 (en) 2004-06-16 2008-03-20 David Lam Compositions and Methods for Enzymatic Decolorization of Chlorophyll
WO2007092314A2 (en) 2006-02-02 2007-08-16 Verenium Corporation Esterases and related nucleic acids and methods
CN100441668C (zh) * 2006-06-09 2008-12-10 中国农业科学院油料作物研究所 超声波强化生物酶油脂脱胶的方法
PT2057274E (pt) 2006-09-21 2014-03-13 Dsm Ip Assets Bv Fosfolípases, ácidos nucleicos que as codificam e métodos de preparação e utilização das mesmas
DE102006046719A1 (de) 2006-10-02 2008-04-03 Ab Enzymes Gmbh Klonierung, Expression und Verwendung saurer Phospholipasen
EP2106280A2 (en) * 2006-12-22 2009-10-07 Danisco US, INC., Genencor Division Enzyme-assisted de-emulsification of aqueous lipid extracts
US8956853B2 (en) 2007-01-30 2015-02-17 Bunge Oils, Inc. Enzymatic degumming utilizing a mixture of PLA and PLC phospholipases
MX2009007919A (es) * 2007-01-30 2009-08-27 Bunge Oils Inc Desgomado enzimatico que utiliza una mezcla de fosfolipasas pla y plc.
US8460905B2 (en) 2007-09-11 2013-06-11 Bunge Oils, Inc. Enzymatic degumming utilizing a mixture of PLA and PLC phospholipases with reduced reaction time
WO2009046988A2 (en) * 2007-10-10 2009-04-16 Chr. Hansen A/S Process for producing a water-in-oil emulsion
US8076123B2 (en) * 2007-10-26 2011-12-13 Oilseeds Biorefinery Corporation Emulsification-free degumming of oil
US8241876B2 (en) 2008-01-07 2012-08-14 Bunge Oils, Inc. Generation of triacylglycerols from gums
GB0904787D0 (en) * 2009-03-20 2009-05-06 Desmet Ballestra Engineering Sa Improved enzymatic oil recuperation process
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UA109884C2 (uk) 2009-10-16 2015-10-26 Поліпептид, що має активність ферменту фосфатидилінозитол-специфічної фосфоліпази с, нуклеїнова кислота, що його кодує, та спосіб його виробництва і застосування
UA111708C2 (uk) 2009-10-16 2016-06-10 Бандж Ойлз, Інк. Спосіб рафінування олії
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US9776105B2 (en) 2010-11-19 2017-10-03 Nalco Company Method for conditioning and processing whole or thin stillage to aid in the separation and recovery of protein and oil fractions
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US9090851B2 (en) 2013-03-13 2015-07-28 Hydrite Chemical Co. Oil extraction method and composition for use in the method
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US9816050B2 (en) 2014-10-27 2017-11-14 Dean Blankenburg Oil extraction method and composition for use in the method
US10377652B1 (en) 2014-12-05 2019-08-13 Fremont Industries, Inc. Process for enhanced fractionation of recovered waste streams
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CN114657017A (zh) * 2022-02-28 2022-06-24 安徽省农业科学院农业工程研究所 一种香榧果实的加工工艺

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3522145A (en) * 1966-07-20 1970-07-28 Colgate Palmolive Co Deodorization of fats
JPS5218797B2 (zh) * 1973-03-10 1977-05-24
JPS5296790A (en) * 1976-02-07 1977-08-13 Mamoru Sugiura Purifying method of enzyme
US4399224A (en) * 1981-07-13 1983-08-16 A. E. Staley Manufacturing Company Enzymatically treated phosphatides
GB8307594D0 (en) * 1983-03-18 1983-04-27 Unilever Plc Triglyceride oils
JP2709736B2 (ja) * 1988-08-11 1998-02-04 昭和産業株式会社 油脂の精製方法
DE4115938A1 (de) * 1991-05-16 1992-11-19 Metallgesellschaft Ag Enzymatisches verfahren zur verminderung des gehaltes an phosphorhaltigen bestandteilen in pflanzlichen und tierischen oelen
JP2937746B2 (ja) * 1993-04-25 1999-08-23 昭和産業株式会社 油脂の精製方法

Cited By (22)

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Publication number Priority date Publication date Assignee Title
USRE43341E1 (en) 1995-06-07 2012-05-01 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
US7972638B2 (en) 1998-07-21 2011-07-05 Danisco A/S Foodstuff
US7718204B2 (en) 1998-07-21 2010-05-18 Danisco A/S Foodstuff
US7781001B2 (en) 1998-07-21 2010-08-24 Danisco A/S Foodstuff
US8163315B2 (en) 1998-07-21 2012-04-24 Danisco A/S Foodstuff
USRE43135E1 (en) 2001-05-18 2012-01-24 Danisco A/S Method of improving dough and bread quality
US8278062B2 (en) 2003-01-14 2012-10-02 Dupont Nutrition Biosciences Aps Method of using lipid acyltransferase
US8003095B2 (en) 2003-01-17 2011-08-23 Danisco A/S Method of using lipid acyltransferase
US7955814B2 (en) 2003-01-17 2011-06-07 Danisco A/S Method
US7955813B2 (en) 2003-01-17 2011-06-07 Danisco, A/S Method of using lipid acyltransferase
US7807398B2 (en) 2003-01-17 2010-10-05 Danisco A/S Method of using lipid acyltransferase
US7718408B2 (en) 2003-12-24 2010-05-18 Danisco A/S Method
US8030044B2 (en) 2003-12-24 2011-10-04 Danisco A/S Lipid acyltransferases
US7906307B2 (en) 2003-12-24 2011-03-15 Danisco A/S Variant lipid acyltransferases and methods of making
US8440435B2 (en) 2003-12-24 2013-05-14 Dupont Nutrition Biosciences Aps Method for reducing 1,2-diglyceride content of an edible oil
US8012732B2 (en) 2004-03-12 2011-09-06 Danisco A/S Fungal lypolytic and amylase enzyme composition and methods using the same
US7666618B2 (en) 2004-07-16 2010-02-23 Danisco A/S Lipolytic enzyme: uses thereof in the food industry
US8192782B2 (en) 2004-07-16 2012-06-05 Danisco A/S Enzymatic oil-degumming method
US8535900B2 (en) 2004-07-16 2013-09-17 Dupont Nutrition Biosciences Aps Lipolytic enzyme uses thereof in the food industry
US8889371B2 (en) 2004-07-16 2014-11-18 Dupont Nutrition Biosciences Aps Lipolytic enzyme: uses thereof in the food industry
US7960150B2 (en) 2007-01-25 2011-06-14 Danisco A/S Production of a lipid acyltransferase from transformed Bacillus licheniformis cells
US8652809B2 (en) 2007-08-17 2014-02-18 Dupont Nutrition Biosciences Aps Method for producing ultra-heat treatment milk

Also Published As

Publication number Publication date
DE4339556C1 (de) 1995-02-02
ES2111841T3 (es) 1998-03-16
DE59405028D1 (de) 1998-02-19
EP0654527A1 (de) 1995-05-24
BR9404496A (pt) 1995-07-11
CN1112156A (zh) 1995-11-22
DK0654527T3 (da) 1998-03-16
GR3026501T3 (en) 1998-07-31
CN1046760C (zh) 1999-11-24
TW279900B (zh) 1996-07-01
ATE162210T1 (de) 1998-01-15
JPH07188691A (ja) 1995-07-25
US5558781A (en) 1996-09-24
CA2136050A1 (en) 1995-05-20

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