EA201890565A1 - Композиции и способы улучшения специфичности в геномной инженерии с применением рнк-направляемых эндонуклеаз - Google Patents
Композиции и способы улучшения специфичности в геномной инженерии с применением рнк-направляемых эндонуклеазInfo
- Publication number
- EA201890565A1 EA201890565A1 EA201890565A EA201890565A EA201890565A1 EA 201890565 A1 EA201890565 A1 EA 201890565A1 EA 201890565 A EA201890565 A EA 201890565A EA 201890565 A EA201890565 A EA 201890565A EA 201890565 A1 EA201890565 A1 EA 201890565A1
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- methods
- endonucleas
- rna
- compositions
- directed
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 2
- 239000000203 mixture Substances 0.000 title 1
- 108020005004 Guide RNA Proteins 0.000 abstract 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 abstract 1
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- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/111—General methods applicable to biologically active non-coding nucleic acids
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- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
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- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B15/00—ICT specially adapted for analysing two-dimensional or three-dimensional molecular structures, e.g. structural or functional relations or structure alignment
- G16B15/10—Nucleic acid folding
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- G16B15/00—ICT specially adapted for analysing two-dimensional or three-dimensional molecular structures, e.g. structural or functional relations or structure alignment
- G16B15/30—Drug targeting using structural data; Docking or binding prediction
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- G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
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- G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
- G16B30/10—Sequence alignment; Homology search
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
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- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
- C12N2740/15041—Use of virus, viral particle or viral elements as a vector
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- Health & Medical Sciences (AREA)
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- Chemical & Material Sciences (AREA)
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- Theoretical Computer Science (AREA)
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- Medicinal Chemistry (AREA)
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- Pharmacology & Pharmacy (AREA)
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- Mycology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Virology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
В данном документе раскрываются оптимизированные направляющие РНК (gRNA) и способы конструирования и применения указанных оптимизированных gRNA, которые характеризуются повышенной специфичностью связывания с мишенью и уменьшенным нецелевым связыванием.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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US201562209466P | 2015-08-25 | 2015-08-25 | |
PCT/US2016/048798 WO2017035416A2 (en) | 2015-08-25 | 2016-08-25 | Compositions and methods of improving specificity in genomic engineering using rna-guided endonucleases |
Publications (1)
Publication Number | Publication Date |
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EA201890565A1 true EA201890565A1 (ru) | 2019-04-30 |
Family
ID=58101224
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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EA201890565A EA201890565A1 (ru) | 2015-08-25 | 2016-08-25 | Композиции и способы улучшения специфичности в геномной инженерии с применением рнк-направляемых эндонуклеаз |
Country Status (13)
Country | Link |
---|---|
US (3) | US11427817B2 (ru) |
EP (4) | EP4177346A3 (ru) |
JP (3) | JP6905755B2 (ru) |
KR (2) | KR20240132120A (ru) |
CN (2) | CN114634930A (ru) |
AU (2) | AU2016311454C1 (ru) |
BR (1) | BR112018003625A2 (ru) |
CA (1) | CA2996001A1 (ru) |
EA (1) | EA201890565A1 (ru) |
ES (1) | ES2929110T3 (ru) |
IL (2) | IL288662B2 (ru) |
MX (1) | MX2018002339A (ru) |
WO (1) | WO2017035416A2 (ru) |
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US9340800B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | Extended DNA-sensing GRNAS |
US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
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US11898179B2 (en) | 2017-03-09 | 2024-02-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
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