EA201792213A1 - Энхансеры продуцирующих клеточных линий - Google Patents

Энхансеры продуцирующих клеточных линий

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Publication number
EA201792213A1
EA201792213A1 EA201792213A EA201792213A EA201792213A1 EA 201792213 A1 EA201792213 A1 EA 201792213A1 EA 201792213 A EA201792213 A EA 201792213A EA 201792213 A EA201792213 A EA 201792213A EA 201792213 A1 EA201792213 A1 EA 201792213A1
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Eurasian Patent Office
Prior art keywords
cell
producing cell
cell lines
edem2
enhancers
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EA201792213A
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English (en)
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Ган ЧЭНЬ
Дарья Бураков
Дипали Дешпанде
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Ридженерон Фармасьютикалз, Инк.
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Application filed by Ридженерон Фармасьютикалз, Инк. filed Critical Ридженерон Фармасьютикалз, Инк.
Publication of EA201792213A1 publication Critical patent/EA201792213A1/ru

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/22—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against growth factors ; against growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C07K14/4705—Regulators; Modulating activity stimulating, promoting or activating activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
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    • C12N15/09—Recombinant DNA-technology
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
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    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/2488—Mannanases
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    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
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    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
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    • C07—ORGANIC CHEMISTRY
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    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
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    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C12N2015/8518—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic expressing industrially exogenous proteins, e.g. for pharmaceutical use, human insulin, blood factors, immunoglobulins, pseudoparticles
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  • Health & Medical Sciences (AREA)
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Abstract

Настоящее изобретение относится к открытию влияния эктопической экспрессии EDEM2 в продуцирующей клетке на улучшение выхода применяемого мультисубъединичного белка. Таким образом, в настоящем изобретении предлагаются продуцирующие клеточные линии, такие как каноническая биофармацевтическая продуцирующая клетка млекопитающего - клетка СНО, содержащая рекомбинантные полинуклеотиды, кодирующие EDEM2. Также раскрыта продуцирующая клетка, содержащая как EDEM2-кодирующий полинуклеотид, так и XBP1-кодирующий полинуклеотид. Раскрыты улучшенные титры антител, продуцированных этими клеточными линиями, а также улучшенные клеточные плотности, приобретенные этими клетками в культуре.
EA201792213A 2012-05-29 2013-05-29 Энхансеры продуцирующих клеточных линий EA201792213A1 (ru)

Applications Claiming Priority (2)

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US201261652549P 2012-05-29 2012-05-29
US13/904,587 US9079954B2 (en) 2012-05-29 2013-05-29 Production cell line enhancers

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EA201792213A1 true EA201792213A1 (ru) 2018-01-31

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EA201792213A EA201792213A1 (ru) 2012-05-29 2013-05-29 Энхансеры продуцирующих клеточных линий
EA201492242A EA028790B1 (ru) 2012-05-29 2013-05-29 Энхансеры продуцирующих клеточных линий

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CA (1) CA2873131A1 (ru)
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FI (1) FI2875047T3 (ru)
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CA2873131A1 (en) 2013-12-05
US10227401B2 (en) 2019-03-12
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US9228012B2 (en) 2016-01-05
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US20150299310A1 (en) 2015-10-22
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US9079954B2 (en) 2015-07-14
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MX2014014630A (es) 2015-02-12
IL235573B (en) 2019-09-26
JP6622334B2 (ja) 2019-12-18
EA201492242A1 (ru) 2015-05-29
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KR20150015492A (ko) 2015-02-10
AU2013267525B2 (en) 2017-11-16
SG10201606654XA (en) 2016-10-28
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CN110835624A (zh) 2020-02-25
JP7037535B2 (ja) 2022-03-16

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