EA200870329A1 - Альдолазы, кодирующие их нуклеиновые кислоты и способы их получения и применения - Google Patents

Альдолазы, кодирующие их нуклеиновые кислоты и способы их получения и применения

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Publication number
EA200870329A1
EA200870329A1 EA200870329A EA200870329A EA200870329A1 EA 200870329 A1 EA200870329 A1 EA 200870329A1 EA 200870329 A EA200870329 A EA 200870329A EA 200870329 A EA200870329 A EA 200870329A EA 200870329 A1 EA200870329 A1 EA 200870329A1
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Eurasian Patent Office
Prior art keywords
polypeptides
activity
methods
monatin
polynucleotides
Prior art date
Application number
EA200870329A
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English (en)
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EA019971B1 (ru
Inventor
Эллен Берк
Паула М. Хикс
Питер Лугинбул
Тоби Ричардсон
Дэвид П. Уэйнер
Лишань Чжао
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Верениум Корпорейшн
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Publication of EA200870329A1 publication Critical patent/EA200870329A1/ru
Publication of EA019971B1 publication Critical patent/EA019971B1/ru

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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1022—Transferases (2.) transferring aldehyde or ketonic groups (2.2)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
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    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/10—Nitrogen as only ring hetero atom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
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    • A—HUMAN NECESSITIES
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    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
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    • C12Y401/03—Oxo-acid-lyases (4.1.3)
    • C12Y401/03016—4-Hydroxy-2-oxoglutarate aldolase (4.1.3.16)
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    • C12Y401/03—Oxo-acid-lyases (4.1.3)
    • C12Y401/03017—4-Hydroxy-4-methyl-2-oxoglutarate aldolase (4.1.3.17)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)
  • Indole Compounds (AREA)

Abstract

Это изобретение относится к полипептидам, обладающим активностью альдолазы, включая активность в отношении пирувата, такую как, но не ограничиваясь этим, активность альдолазы HMG и/или KHG, к полинуклеотидам, кодирующим эти полипептиды, и к способам получения и применения этих полинуклеотидов и полипептидов. В некоторых вариантах осуществления это изобретение относится к полипептидам, обладающим активностью альдолазы, включая активность в отношении пирувата, такую как, но не ограничиваясь этим, активность альдолазы HMG и/или KHG, включая термостабильную или термоустойчивую активность, и к полинуклеотидам, кодирующим эти ферменты, и к получению и применению этих полинуклеотидов и полипептидов. Полипептиды по этому изобретению можно применять во множестве фармацевтических, сельскохозяйственных и промышленных направлений. В некоторых вариантах осуществления это изобретение относится к полипептидам и к биосинтетическим каскадам, которые пригодны для получения R-2-гидрокси-2-(индол-3-илметил)-4-кетоглутаровой кислоты (R-MP) и определенных стереоизомеров монатина, таких как R,R- и S,R-монатин, и их солей, а также определенных стереоизомеров производных монатина, таких как R,R- и S,R-конфигурации, и их солей.
EA200870329A 2006-03-07 2007-03-07 Альдолазы, кодирующие их нуклеиновые кислоты и способы их получения и применения EA019971B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US78051506P 2006-03-07 2006-03-07
PCT/US2007/063515 WO2007103989A2 (en) 2006-03-07 2007-03-07 Aldolases, nucleic acids encoding them and methods for making and using them

Publications (2)

Publication Number Publication Date
EA200870329A1 true EA200870329A1 (ru) 2009-06-30
EA019971B1 EA019971B1 (ru) 2014-07-30

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ID=38475839

Family Applications (2)

Application Number Title Priority Date Filing Date
EA201301267A EA029538B1 (ru) 2006-03-07 2007-03-07 Полипептид, обладающий активностью альдолазы, полинуклеотид, кодирующий этот полипептид, и способы получения и применения полинуклеотида и полипептида
EA200870329A EA019971B1 (ru) 2006-03-07 2007-03-07 Альдолазы, кодирующие их нуклеиновые кислоты и способы их получения и применения

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EA201301267A EA029538B1 (ru) 2006-03-07 2007-03-07 Полипептид, обладающий активностью альдолазы, полинуклеотид, кодирующий этот полипептид, и способы получения и применения полинуклеотида и полипептида

Country Status (13)

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US (3) US8546118B2 (ru)
EP (4) EP3153580A3 (ru)
JP (3) JP5733777B2 (ru)
KR (4) KR101588539B1 (ru)
CN (3) CN102690833B (ru)
AT (1) ATE548450T1 (ru)
AU (1) AU2007223086B2 (ru)
BR (2) BRPI0708614A2 (ru)
CA (1) CA2645225A1 (ru)
EA (2) EA029538B1 (ru)
ES (1) ES2383767T3 (ru)
MX (1) MX2008011442A (ru)
WO (1) WO2007103989A2 (ru)

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