EA029777B1 - Ячмень и напитки на основе солода с низкими концентрациями dms - Google Patents
Ячмень и напитки на основе солода с низкими концентрациями dms Download PDFInfo
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- EA029777B1 EA029777B1 EA201170739A EA201170739A EA029777B1 EA 029777 B1 EA029777 B1 EA 029777B1 EA 201170739 A EA201170739 A EA 201170739A EA 201170739 A EA201170739 A EA 201170739A EA 029777 B1 EA029777 B1 EA 029777B1
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- barley
- mmt
- plant
- mutant
- malt
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
- C12N9/1007—Methyltransferases (general) (2.1.1.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
- C12C1/00—Preparation of malt
- C12C1/18—Preparation of malt extract or of special kinds of malt, e.g. caramel, black malt
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
- C12C12/00—Processes specially adapted for making special kinds of beer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
- C12C2200/00—Special features
- C12C2200/01—Use of specific genetic variants of barley or other sources of fermentable carbohydrates for beer brewing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y201/00—Transferases transferring one-carbon groups (2.1)
- C12Y201/01—Methyltransferases (2.1.1)
- C12Y201/01012—Methionine S-methyltransferase (2.1.1.12)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Nutrition Science (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Non-Alcoholic Beverages (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Noodles (AREA)
- Cereal-Derived Products (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DKPA200801708 | 2008-12-03 | ||
| PCT/DK2009/050315 WO2010063288A2 (en) | 2008-12-03 | 2009-12-01 | Barley and malt-derived beverages with low dms level |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| EA201170739A1 EA201170739A1 (ru) | 2012-01-30 |
| EA029777B1 true EA029777B1 (ru) | 2018-05-31 |
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Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9587210B2 (en) | 2008-12-03 | 2017-03-07 | Carlsberg Breweries A/S | Energy saving brewing method |
| BR112012030806B8 (pt) | 2010-06-03 | 2022-05-10 | Carlsberg Breweries As | Método para preparar uma bebida à base de cevada e composição de malte moído |
| KR102433243B1 (ko) | 2016-07-01 | 2022-08-17 | 칼스버그 브류어리스 에이/에스 | 정제 곡물계 음료 |
| RU2733294C1 (ru) | 2016-11-18 | 2020-10-01 | Балтика Брюэрис - Парт Оф Зе Карлсберг Груп | Способ получения зернового солода и солодовый продукт, полученный таким способом |
| JP7116536B2 (ja) * | 2017-10-17 | 2022-08-10 | サッポロビール株式会社 | ビールテイスト飲料、ビールテイスト飲料の製造方法及びビールテイスト飲料の高級感を改善する方法 |
| MA51410A (fr) | 2017-12-28 | 2021-04-07 | Carlsberg As | Plantes céréalières présentant des propriétés de paroi cellulaire améliorées |
| HUE066457T2 (hu) | 2017-12-28 | 2024-08-28 | Carlsberg As | Gyors módszerek gabona kivonatok készítéséhez |
| CA3086078A1 (en) | 2017-12-28 | 2019-07-04 | Carlsberg A/S | Method for producing an extract of cereal and method for processing this extract into beverage |
| CA3085899A1 (en) | 2017-12-28 | 2019-07-04 | Carlsberg A/S | Barley with increased hydrolytic enzyme activity |
| EP3784058A1 (en) | 2018-04-25 | 2021-03-03 | Carlsberg A/S | Barley based beverages |
| CN112345654A (zh) * | 2019-08-06 | 2021-02-09 | 红塔烟草(集团)有限责任公司 | 一种基于色谱指纹图谱识别油斑烟污染源的方法 |
| WO2021149040A1 (en) * | 2020-01-22 | 2021-07-29 | Ramot At Tel-Aviv University Ltd. | Anti-ide antibodies and uses of same |
| MX2022010777A (es) * | 2020-03-02 | 2022-11-30 | Carlsberg As | Plantas de cebada con elevada actividad de dextrinasa limite. |
| CN112795545B (zh) * | 2021-01-29 | 2022-04-12 | 浙江大学 | 大麦HvHMT3基因及其应用 |
| AR132565A1 (es) | 2023-05-01 | 2025-07-16 | Carlsberg As | Cebada con propiedades mejoradas |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2220955A (en) * | 1988-06-04 | 1990-01-24 | Carlsberg As | A method of brewing beer using a mutant barley |
| US5242694A (en) * | 1992-02-18 | 1993-09-07 | G. Heileman Brewing Company, Inc. | Process for brewing low carbohydrate near beer |
| AU3857893A (en) * | 1992-05-14 | 1993-11-18 | Joe White Maltings Limited | Malt production |
| WO2000015757A2 (en) * | 1998-09-15 | 2000-03-23 | Carlsberg A/S | Method of producing a composite fermented beverage using genetically modified yeast strains |
| WO2002053721A1 (en) * | 2000-12-29 | 2002-07-11 | Carlsberg Research Laboratory | Low-lipoxygenase 1 barley |
| US20050204437A1 (en) * | 2004-03-11 | 2005-09-15 | Carlsberg A/S | Barley for production of flavor-stable beverage |
| US20060057684A1 (en) * | 2002-07-25 | 2006-03-16 | Novozymes A/S | Mashing process |
| DE202007005281U1 (de) * | 2007-04-11 | 2007-07-05 | Hertel, Marcus, Dipl.-Ing. | Vorrichtung zur Niedertemperaturwürzekochung |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9722027D0 (en) | 1997-10-17 | 1997-12-17 | Melica Hb | Methods |
| AU3005300A (en) | 1999-02-22 | 2000-09-04 | Michigan State University | Compositions and methods for altering sulfur content in plants |
| US6660915B2 (en) * | 2000-12-29 | 2003-12-09 | Anna Christina Douma | Low lipoxygenase 1 barley |
| US7323338B2 (en) | 2001-05-02 | 2008-01-29 | Gavish-Galilee Bio Applications Ltd. | Plants characterized by an increased content of methionine and related metabolites, methods of generating same and uses thereof |
| JP4113795B2 (ja) | 2003-03-25 | 2008-07-09 | サッポロビール株式会社 | 大麦リポキシゲナーゼ−1遺伝子、大麦の選抜方法、麦芽アルコール飲料用原料及び麦芽アルコール飲料の製造方法 |
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2009
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- 2009-12-01 EP EP09771493.5A patent/EP2373154B1/en active Active
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Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2220955A (en) * | 1988-06-04 | 1990-01-24 | Carlsberg As | A method of brewing beer using a mutant barley |
| US5242694A (en) * | 1992-02-18 | 1993-09-07 | G. Heileman Brewing Company, Inc. | Process for brewing low carbohydrate near beer |
| AU3857893A (en) * | 1992-05-14 | 1993-11-18 | Joe White Maltings Limited | Malt production |
| WO2000015757A2 (en) * | 1998-09-15 | 2000-03-23 | Carlsberg A/S | Method of producing a composite fermented beverage using genetically modified yeast strains |
| WO2002053721A1 (en) * | 2000-12-29 | 2002-07-11 | Carlsberg Research Laboratory | Low-lipoxygenase 1 barley |
| US20060057684A1 (en) * | 2002-07-25 | 2006-03-16 | Novozymes A/S | Mashing process |
| US20050204437A1 (en) * | 2004-03-11 | 2005-09-15 | Carlsberg A/S | Barley for production of flavor-stable beverage |
| DE202007005281U1 (de) * | 2007-04-11 | 2007-07-05 | Hertel, Marcus, Dipl.-Ing. | Vorrichtung zur Niedertemperaturwürzekochung |
Non-Patent Citations (2)
| Title |
|---|
| HIROTA N; KURODA H; TAKOI K; KANEKO T; KANEDA H; YOSHIDA I; TAKASHIO M; ITO K; TAKEDA K: "Brewing performance of malted lipoxygenase-1 null barley and effect on the flavor stability of beer", CEREAL CHEMISTRY, AACC INTERNATIONAL INC., US, vol. 83, no. 3, 1 May 2006 (2006-05-01), US, pages 250 - 254, XP009124704, ISSN: 0009-0352, DOI: 10.1094/CC-83-0250 * |
| HOTZEL H, M�LLER W, SACHSE K: "Recovery and characterization of residual DNA from beer as a prerequisite for the detection of genetically modified ingredients", EUROPEAN FOOD RESEARCH AND TECHNOLOGY, SPRINGER BERLIN HEIDELBERG, BERLIN/HEIDELBERG, vol. 209, no. 3-4, 1 January 1999 (1999-01-01), Berlin/Heidelberg, pages 192 - 196, XP002635202, ISSN: 1438-2377, DOI: 10.1007/s002170050478 * |
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