EA004317B1 - Получение рекомбинантных факторов свертывания крови в линиях клеток человека - Google Patents

Получение рекомбинантных факторов свертывания крови в линиях клеток человека Download PDF

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Publication number
EA004317B1
EA004317B1 EA200201008A EA200201008A EA004317B1 EA 004317 B1 EA004317 B1 EA 004317B1 EA 200201008 A EA200201008 A EA 200201008A EA 200201008 A EA200201008 A EA 200201008A EA 004317 B1 EA004317 B1 EA 004317B1
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Prior art keywords
factor viii
vector
mutein
zeg
sss
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EA200201008A
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English (en)
Russian (ru)
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EA200201008A1 (ru
Inventor
Шарлотта Хаузер
Андреа Херстер
Карола Шредер
Михель Ленерер
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Октагене Гмбх
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Priority claimed from EP00106225A external-priority patent/EP1136553A1/en
Application filed by Октагене Гмбх filed Critical Октагене Гмбх
Publication of EA200201008A1 publication Critical patent/EA200201008A1/ru
Publication of EA004317B1 publication Critical patent/EA004317B1/ru

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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424Serine endopeptidases (3.4.21)
    • C12N9/6437Coagulation factor VIIa (3.4.21.21)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00Drugs for disorders of the blood or the extracellular fluid
    • A61P7/04Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
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    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745Blood coagulation or fibrinolysis factors
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745Blood coagulation or fibrinolysis factors
    • C07K14/755Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424Serine endopeptidases (3.4.21)
    • C12N9/644Coagulation factor IXa (3.4.21.22)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424Serine endopeptidases (3.4.21)
    • C12N9/647Blood coagulation factors not provided for in a preceding group or according to more than one of the proceeding groups
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y304/00Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21Serine endopeptidases (3.4.21)
    • C12Y304/21021Coagulation factor VIIa (3.4.21.21)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y304/00Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21Serine endopeptidases (3.4.21)
    • C12Y304/21022Coagulation factor IXa (3.4.21.22)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy

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  • Health & Medical Sciences (AREA)
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  • Organic Chemistry (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
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  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
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  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Diabetes (AREA)
  • Public Health (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
EA200201008A 2000-03-22 2001-03-21 Получение рекомбинантных факторов свертывания крови в линиях клеток человека EA004317B1 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP00106225A EP1136553A1 (en) 2000-03-22 2000-03-22 Production of recombinant blood clotting factors in human cell lines
US20324900P 2000-05-08 2000-05-08
PCT/EP2001/003220 WO2001070968A2 (en) 2000-03-22 2001-03-21 Production of recombinant blood clotting factors in human cell lines

Publications (2)

Publication Number Publication Date
EA200201008A1 EA200201008A1 (ru) 2003-02-27
EA004317B1 true EA004317B1 (ru) 2004-02-26

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EA200201008A EA004317B1 (ru) 2000-03-22 2001-03-21 Получение рекомбинантных факторов свертывания крови в линиях клеток человека

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US (1) US7572619B2 (enExample)
EP (2) EP1460131A3 (enExample)
JP (1) JP3894795B2 (enExample)
KR (1) KR100581574B1 (enExample)
CN (1) CN1454257B (enExample)
AT (1) ATE312176T1 (enExample)
AU (3) AU2001254715B2 (enExample)
BE (1) BE2014C077I2 (enExample)
BG (1) BG65930B1 (enExample)
BR (1) BRPI0109494B8 (enExample)
CA (1) CA2404163C (enExample)
CZ (1) CZ303929B6 (enExample)
DE (1) DE60115613T2 (enExample)
DK (1) DK1266006T3 (enExample)
EA (1) EA004317B1 (enExample)
EE (1) EE200200538A (enExample)
ES (1) ES2254403T3 (enExample)
FR (1) FR15C0003I2 (enExample)
HR (1) HRP20020767B1 (enExample)
HU (1) HU228091B1 (enExample)
IL (2) IL151857A0 (enExample)
MX (1) MXPA02009221A (enExample)
NO (1) NO330910B1 (enExample)
NZ (1) NZ521732A (enExample)
RS (1) RS50743B (enExample)
SI (1) SI1266006T1 (enExample)
SK (1) SK287706B6 (enExample)
WO (1) WO2001070968A2 (enExample)

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US11267868B2 (en) 2013-04-02 2022-03-08 Chugai Seiyaku Kabushiki Kaisha Fc region variant
US11454633B2 (en) 2014-12-19 2022-09-27 Chugai Seiyaku Kabushiki Kaisha Anti-myostatin antibodies, polypeptides containing variant Fc regions, and methods of use
US11718678B2 (en) 2011-02-25 2023-08-08 Chugai Seiyaku Kabushiki Kaisha Method for altering plasma retention and immunogenicity of antigen-binding molecule
US11780912B2 (en) 2016-08-05 2023-10-10 Chugai Seiyaku Kabushiki Kaisha Composition for prophylaxis or treatment of IL-8 related diseases
US11820793B2 (en) 2011-11-30 2023-11-21 Chugai Seiyaku Kabushiki Kaisha Drug containing carrier into cell for forming immune complex
US11827699B2 (en) 2011-09-30 2023-11-28 Chugai Seiyaku Kabushiki Kaisha Methods for producing antibodies promoting disappearance of antigens having plurality of biological activities
US12084513B2 (en) 2017-11-14 2024-09-10 Chugai Seiyaku Kabushiki Kaisha Anti-C1S antibodies and methods of use
US12252532B2 (en) 2015-12-25 2025-03-18 Chugai Seiyaku Kabushiki Kaisha Anti-myostatin antibodies and methods of use
US12304960B2 (en) 2018-08-10 2025-05-20 Chugai Seiyaku Kabushiki Kaisha Anti-CD137 antigen-binding molecule and utilization thereof
US12371511B2 (en) 2011-06-30 2025-07-29 Chugai Seiyaku Kabushiki Kaisha Heterodimerized polypeptide
US12473353B2 (en) 2019-05-15 2025-11-18 Chugai Seiyaku Kabushiki Kaisha Antigen-binding molecule, a pharmaceutical composition, and a method

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US12371511B2 (en) 2011-06-30 2025-07-29 Chugai Seiyaku Kabushiki Kaisha Heterodimerized polypeptide
US11827699B2 (en) 2011-09-30 2023-11-28 Chugai Seiyaku Kabushiki Kaisha Methods for producing antibodies promoting disappearance of antigens having plurality of biological activities
US11820793B2 (en) 2011-11-30 2023-11-21 Chugai Seiyaku Kabushiki Kaisha Drug containing carrier into cell for forming immune complex
US11267868B2 (en) 2013-04-02 2022-03-08 Chugai Seiyaku Kabushiki Kaisha Fc region variant
US11454633B2 (en) 2014-12-19 2022-09-27 Chugai Seiyaku Kabushiki Kaisha Anti-myostatin antibodies, polypeptides containing variant Fc regions, and methods of use
US12169205B2 (en) 2014-12-19 2024-12-17 Chugai Seiyaku Kabushiki Kaisha Anti-myostatin antibodies, polypeptides containing variant fc regions, and methods of use
US12252532B2 (en) 2015-12-25 2025-03-18 Chugai Seiyaku Kabushiki Kaisha Anti-myostatin antibodies and methods of use
US11780912B2 (en) 2016-08-05 2023-10-10 Chugai Seiyaku Kabushiki Kaisha Composition for prophylaxis or treatment of IL-8 related diseases
US12084513B2 (en) 2017-11-14 2024-09-10 Chugai Seiyaku Kabushiki Kaisha Anti-C1S antibodies and methods of use
US12304960B2 (en) 2018-08-10 2025-05-20 Chugai Seiyaku Kabushiki Kaisha Anti-CD137 antigen-binding molecule and utilization thereof
US12473353B2 (en) 2019-05-15 2025-11-18 Chugai Seiyaku Kabushiki Kaisha Antigen-binding molecule, a pharmaceutical composition, and a method

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FR15C0003I1 (fr) 2015-02-27
BG107152A (bg) 2003-06-30
SK287706B6 (en) 2011-07-06
JP3894795B2 (ja) 2007-03-22
WO2001070968A3 (en) 2001-12-13
EA200201008A1 (ru) 2003-02-27
IL151857A (en) 2010-05-31
WO2001070968A2 (en) 2001-09-27
BRPI0109494B8 (pt) 2021-05-25
DE60115613D1 (de) 2006-01-12
KR20030011079A (ko) 2003-02-06
EE200200538A (et) 2004-04-15
DE60115613T2 (de) 2006-08-24
HUP0300588A2 (hu) 2003-06-28
HRP20020767A2 (en) 2005-02-28
EP1460131A3 (en) 2005-06-01
DK1266006T3 (da) 2006-01-16
US20040023333A1 (en) 2004-02-05
CA2404163C (en) 2009-09-29
AU5471501A (en) 2001-10-03
BE2014C077I2 (enExample) 2020-01-30
ES2254403T3 (es) 2006-06-16
HUP0300588A3 (en) 2005-09-28
AU2006201848A1 (en) 2006-05-25
YU71002A (sh) 2005-11-28
AU2001254715B2 (en) 2006-02-02
SK15042002A3 (sk) 2003-09-11
FR15C0003I2 (fr) 2015-11-20
JP2003530093A (ja) 2003-10-14
BRPI0109494B1 (pt) 2021-05-11
NO20024475L (no) 2002-11-20
NO330910B1 (no) 2011-08-15
IL151857A0 (en) 2003-04-10
BR0109494A (pt) 2002-12-10
EP1266006B1 (en) 2005-12-07
SI1266006T1 (sl) 2006-06-30
NZ521732A (en) 2004-05-28
RS50743B (sr) 2010-08-31
CN1454257B (zh) 2013-01-16
MXPA02009221A (es) 2005-07-25
EP1266006A2 (en) 2002-12-18
CN1454257A (zh) 2003-11-05
BG65930B1 (bg) 2010-05-31
HU228091B1 (en) 2012-10-29
CZ20023166A3 (cs) 2003-02-12
NO20024475D0 (no) 2002-09-19
ATE312176T1 (de) 2005-12-15
KR100581574B1 (ko) 2006-05-22
US7572619B2 (en) 2009-08-11
CA2404163A1 (en) 2001-09-27
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HRP20020767B1 (hr) 2011-09-30
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