DK496385A - Reguleringsregion for heterolog genekspression i gaer - Google Patents

Reguleringsregion for heterolog genekspression i gaer Download PDF

Info

Publication number
DK496385A
DK496385A DK496385A DK496385A DK496385A DK 496385 A DK496385 A DK 496385A DK 496385 A DK496385 A DK 496385A DK 496385 A DK496385 A DK 496385A DK 496385 A DK496385 A DK 496385A
Authority
DK
Denmark
Prior art keywords
yeast
gene expression
heterologous gene
regulation region
regulation
Prior art date
Application number
DK496385A
Other languages
Danish (da)
English (en)
Other versions
DK496385D0 (da
Inventor
David Womack Stroman
Paul Frederick Brust
Steven Bradley Ellis
Thomas Raymond Gingeras
Michael Miller Harpold
Juerg Friedrich Tschopp
Original Assignee
Phillips Petroleum Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US06/666,391 external-priority patent/US4808537A/en
Application filed by Phillips Petroleum Co filed Critical Phillips Petroleum Co
Publication of DK496385D0 publication Critical patent/DK496385D0/da
Publication of DK496385A publication Critical patent/DK496385A/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/68—Stabilisation of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
DK496385A 1984-10-30 1985-10-29 Reguleringsregion for heterolog genekspression i gaer DK496385A (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US06/666,391 US4808537A (en) 1984-10-30 1984-10-30 Methanol inducible genes obtained from pichia and methods of use
US06/780,102 US4855231A (en) 1984-10-30 1985-09-25 Regulatory region for heterologous gene expression in yeast

Publications (2)

Publication Number Publication Date
DK496385D0 DK496385D0 (da) 1985-10-29
DK496385A true DK496385A (da) 1986-05-01

Family

ID=27099454

Family Applications (1)

Application Number Title Priority Date Filing Date
DK496385A DK496385A (da) 1984-10-30 1985-10-29 Reguleringsregion for heterolog genekspression i gaer

Country Status (23)

Country Link
US (1) US4855231A (zh)
EP (2) EP0183071B1 (zh)
JP (5) JP2502508B2 (zh)
KR (1) KR930001117B1 (zh)
CN (1) CN1011243B (zh)
AR (1) AR242988A1 (zh)
AT (1) ATE83263T1 (zh)
AU (1) AU572002B2 (zh)
CA (1) CA1340733C (zh)
DD (1) DD254211A5 (zh)
DE (1) DE3586889T2 (zh)
DK (1) DK496385A (zh)
ES (1) ES8609458A1 (zh)
FI (1) FI94427C (zh)
GR (1) GR852611B (zh)
HU (1) HU205627B (zh)
IE (1) IE930804L (zh)
IL (1) IL76765A (zh)
NO (1) NO178076C (zh)
NZ (1) NZ213842A (zh)
PL (1) PL158965B1 (zh)
PT (1) PT81399B (zh)
YU (1) YU46695B (zh)

Families Citing this family (116)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5741672A (en) * 1984-07-27 1998-04-21 Unilever Patent Holdings B.V. Expression and production of polypeptides using the promoters of the hansenula polymorpha MOX and DAS genes
US4879231A (en) * 1984-10-30 1989-11-07 Phillips Petroleum Company Transformation of yeasts of the genus pichia
US4818700A (en) * 1985-10-25 1989-04-04 Phillips Petroleum Company Pichia pastoris argininosuccinate lyase gene and uses thereof
US4882279A (en) * 1985-10-25 1989-11-21 Phillips Petroleum Company Site selective genomic modification of yeast of the genus pichia
US5032516A (en) * 1985-10-25 1991-07-16 Phillips Petroleum Company Pichia pastoris alcohol oxidase II regulatory region
US4895800A (en) * 1985-11-26 1990-01-23 Phillips Petroleum Company Yeast production of hepatitis B surface antigen
IL81817A0 (en) * 1986-03-14 1987-10-20 Phillips Petroleum Co Methanol and glucose responsive yeast regulatory regions
ZA872534B (en) * 1986-05-08 1987-11-25 Phillips Petroleum Co Yeast production of streptokinase
IL82935A0 (en) * 1986-08-12 1987-12-20 Phillips Petroleum Co Secretion of heterologous proteins from yeast
US5002876A (en) * 1986-09-22 1991-03-26 Phillips Petroleum Company Yeast production of human tumor necrosis factor
ATE105858T1 (de) * 1987-07-17 1994-06-15 Rhein Biotech Ges Fuer Biotech Dna-moleküle, die für fmdh-kontrollabschnitte und strukturgene für ein protein mit fmdh-aktivität kodieren, sowie deren anwendung.
US5179003A (en) * 1988-01-05 1993-01-12 Boehringer Mannheim Gmbh Process for the production of proteins or protein-containing gene products
FI891695A (fi) * 1988-04-11 1989-10-12 Phillips Petroleum Co Expression av laxtillvaexthormon i methyltrofisk jaest av slaektet pichia.
IL89989A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Expression of human interleukin-2 in methylotrophic yeasts
IL89993A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Expression of the hiv 24kda gag protein in methylotrophic yeasts
IL90021A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Process for the production of interferon
IL89991A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Expression of hepatitis b pres2 protein in methylotrophic yeasts
IL89992A0 (en) 1988-04-25 1989-12-15 Phillips Petroleum Co Expression of human serum albumin in methylotrophic yeasts
ATE111524T1 (de) * 1988-12-20 1994-09-15 Rhein Biotech Ges Fuer Biotech Mutanter stamm von einem methylotrophen organismus und verfahren zur herstellung eines proteins in methylotrophen organismus.
AU5196290A (en) * 1989-02-13 1990-09-05 Salk Institute Biotechnology/Industrial Associates, Inc., The Production of superoxide dismutase in pichia pastoris yeast cells
GB8924883D0 (en) * 1989-11-03 1989-12-20 Shell Int Research Expression vector and polypeptide synthesis
US5264554A (en) * 1990-01-19 1993-11-23 The Blood Center Of Southeastern Wisconsin, Inc. Platelet cell adhesion molecule and variants thereof
US5369028A (en) * 1990-04-03 1994-11-29 The Salk Institute Biotechnology/Industrial Associates, Inc. DNA and mRNA encoding human neuronal nicotinic acetylcholine receptor compositions and cells transformed with same
US6440681B1 (en) 1990-04-03 2002-08-27 Merck & Co., Inc. Methods for identifying agonists and antagonists for human neuronal nicotinic acetylcholine receptors
US5258302A (en) * 1990-07-03 1993-11-02 The Salk Institute Biotechnology/Industrial Associates, Inc. DNA for expression of aprotinin in methylotrophic yeast cells
JPH06500470A (ja) * 1990-09-04 1994-01-20 メルク　エンド　カンパニー　インコーポレーテッド メチロトローフ酵母細胞におけるインスリン様成長因子の産生
US5612198A (en) * 1990-09-04 1997-03-18 The Salk Institute Production of insulin-like growth factor-1 in methylotrophic yeast cells
US5268273A (en) * 1990-12-14 1993-12-07 Phillips Petroleum Company Pichia pastoris acid phosphatase gene, gene regions, signal sequence and expression vectors comprising same
WO1992013951A1 (en) * 1991-02-04 1992-08-20 The Salk Institute Biotechnology/Industrial Associates, Inc. Production of human serum albumin in methylotrophic yeast cells
EP0578746B1 (en) * 1991-04-01 2002-07-03 Merck & Co., Inc. GENES WHICH INFLUENCE $i(PICHIA) PROTEOLYTIC ACTIVITY, AND USES THEREFOR
CA2058820C (en) * 1991-04-25 2003-07-15 Kotikanyad Sreekrishna Expression cassettes and vectors for the secretion of human serum albumin in pichia pastoris cells
US5330901A (en) * 1991-04-26 1994-07-19 Research Corporation Technologies, Inc. Expression of human serum albumin in Pichia pastoris
US5834262A (en) * 1992-01-06 1998-11-10 E. I. Du Pont De Nemours And Company Oxidation of glycolic acid to glyoxylic acid using a microbial cell transformant as catalyst
EP0558024B1 (en) * 1992-02-28 1996-08-14 Suntory Limited Novel vector having promoter that is inducible by methanol and/or glycerol
US5708141A (en) * 1992-05-11 1998-01-13 Corvas International, Inc. Neutrophil inhibitors
CA2158017C (en) * 1993-03-03 2005-05-17 David Leroy Anton Production of glycolate oxidase in methylotrophic yeast
WO1994020617A2 (en) 1993-03-08 1994-09-15 The Salk Institute Biotechnology/Industrial Associates, Incorporated Human neuronal nicotinic acetylcholine receptor compositions and methods employing same
AU6415594A (en) * 1993-03-25 1994-10-11 Biosource Genetics Corporation (pichia pastoris) alcohol oxidase (zza1) and (zza2)
JPH08509607A (ja) * 1993-04-20 1996-10-15 ザ ソールク インスチチュート バイオテクノロジー／インダストリアル アソシエイツ，インコーポレイテッド ヒトｎ−メチル−ｄ−アスパラギン酸受容体サブユニット、これをコードする核酸およびその用途
US6001581A (en) * 1993-06-04 1999-12-14 Sibia Neurosciences, Inc. Cation-based bioassay using human metabotropic glutamate receptors
US5521297A (en) 1993-06-04 1996-05-28 Salk Institute Biotechnology/Industrial Associates Nucleic acids encoding human metabotropic glutamate receptors
US5912122A (en) * 1993-06-04 1999-06-15 Sibia Neurosciences, Inc. Nucleic acids encoding and method for detecting nucleic acid encoding human metabotropic glutamate receptor subtype mGluR6
US6638905B2 (en) 1993-06-18 2003-10-28 The Salk Institute For Biological Studies Cloning and recombinant production of CFR receptor(s)
US6495343B1 (en) 1993-06-18 2002-12-17 The Salk Institute For Biological Studies Cloning and recombinant production of CRF receptor(s)
WO1995013299A1 (en) * 1993-11-08 1995-05-18 The Salk Institute Biotechnology/Industrial Associates, Inc. Human neuronal nicotinic acetylcholine receptor compositions and methods employing same
US5453161A (en) * 1993-11-12 1995-09-26 Enichem S.P.A. Polyamic acid to polyimide conversion by microwave heating
US5863894A (en) * 1995-06-05 1999-01-26 Corvas International, Inc. Nematode-extracted anticoagulant protein
US5866543A (en) * 1995-06-05 1999-02-02 Corvas International, Inc. Nematode-extracted anticoagulant protein
US5872098A (en) * 1995-06-05 1999-02-16 Corvas International, Inc. Nematode-extracted anticoagulant protein
US5945275A (en) * 1994-10-18 1999-08-31 Corvas International, Inc. Nematode-extracted anticoagulant protein
CA2202351A1 (en) * 1994-10-18 1996-04-25 George Phillip Vlasuk Nematode-extracted serine protease inhibitors and anticoagulant proteins
PL183855B1 (pl) * 1994-10-18 2002-07-31 Corvas International Białko, cząsteczka cDNA, oligonukleotyd, kompozycja farmaceutyczna i środek farmaceutyczny do hamowania krzepnięcia krwi
US5866542A (en) * 1994-10-18 1999-02-02 Corvas International, Inc. Nematode-extracted anticoagulant protein
US6485967B1 (en) 1995-06-07 2002-11-26 Merck & Co., Inc. Human neuronal nicotinic acetylcholine receptor α6 and β3 nucleic acid
US6143557A (en) * 1995-06-07 2000-11-07 Life Technologies, Inc. Recombination cloning using engineered recombination sites
US6720140B1 (en) * 1995-06-07 2004-04-13 Invitrogen Corporation Recombinational cloning using engineered recombination sites
EP0889966A1 (en) * 1995-11-09 1999-01-13 ZymoGenetics, Inc. Compositions and methods for producing heterologous polypeptides in pichia methanolica
MX9605082A (es) 1996-10-24 1998-04-30 Univ Autonoma De Nuevo Leon Levaduras metilotroficas modificadas geneticamente para la produccion y secrecion de hormona de crecimiento humano.
EP1659173A1 (en) 1997-10-01 2006-05-24 DSMIP Assets B.V. Protein production process
NZ533288A (en) * 1997-10-24 2005-10-28 Invitrogen Corp Recombinational cloning using nucleic acids having recombination sites
CN101125873A (zh) * 1997-10-24 2008-02-20 茵维特罗根公司 利用具重组位点的核酸进行重组克隆
US7351578B2 (en) * 1999-12-10 2008-04-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
WO2000001731A1 (fr) * 1998-07-06 2000-01-13 Tosoh Corporation Proteine fusionnee avec liaison directe du recepteur il-6 a il-6
WO2000012687A1 (en) * 1998-08-28 2000-03-09 Invitrogen Corporation System for the rapid manipulation of nucleic acid sequences
US6780615B1 (en) 1998-12-31 2004-08-24 Genway Biotech Inc. Production of recombinant monellin using methylotrophic yeast expression system
US6720174B1 (en) 1999-01-28 2004-04-13 Novozymes A/S Phytases
AU774643B2 (en) 1999-03-02 2004-07-01 Invitrogen Corporation Compositions and methods for use in recombinational cloning of nucleic acids
US7504253B2 (en) * 1999-06-11 2009-03-17 The Burnham Institute For Medical Research Nucleic acid encoding proteins involved in protein degradation, products and methods related thereof
US6261820B1 (en) * 1999-10-01 2001-07-17 Amgen Inc. Fibronolytically active polypeptide
US6440414B1 (en) * 1999-10-01 2002-08-27 Amgen Inc. Pharmaceutical compositions of fibrinolytic agent
AU1591701A (en) 1999-11-12 2001-06-06 Fibrogen, Inc. Recombinant gelatins
WO2001042509A1 (en) 1999-12-10 2001-06-14 Invitrogen Corporation Use of multiple recombination sites with unique specificity in recombinational cloning
US7033776B2 (en) * 1999-12-17 2006-04-25 Amgen Inc. Method for treatment of indwelling catheter occlusion using fibrinolytic metalloproteinases
WO2001077351A1 (en) * 2000-04-07 2001-10-18 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Vectors and methods for dual protein expression in pichia pastoris and escherichia coli
US7244560B2 (en) * 2000-05-21 2007-07-17 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US7863020B2 (en) 2000-06-28 2011-01-04 Glycofi, Inc. Production of sialylated N-glycans in lower eukaryotes
EP1522590B1 (en) 2000-06-28 2009-08-26 Glycofi, Inc. Methods for producing modified glycoproteins
US7598055B2 (en) * 2000-06-28 2009-10-06 Glycofi, Inc. N-acetylglucosaminyltransferase III expression in lower eukaryotes
US8697394B2 (en) * 2000-06-28 2014-04-15 Glycofi, Inc. Production of modified glycoproteins having multiple antennary structures
US7449308B2 (en) 2000-06-28 2008-11-11 Glycofi, Inc. Combinatorial DNA library for producing modified N-glycans in lower eukaryotes
US7625756B2 (en) 2000-06-28 2009-12-01 GycoFi, Inc. Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells
US6803225B2 (en) 2000-06-30 2004-10-12 Flanders Interuniversity Institute For Biotechnology Protein glycosylation modification in Pichia pastoris
US7009045B2 (en) * 2000-07-14 2006-03-07 Archer-Daniels-Midland Company Transformation systems for flavinogenic yeast
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US6699691B2 (en) 2001-04-05 2004-03-02 The Board Of Regents Of The University Of Nebraska Alcohol oxidase 1 regulatory nucleotide sequences for heterologous gene expression in yeast
CA2448505A1 (en) * 2001-05-21 2002-11-28 Invitrogen Corporation Compositions and methods for use in isolation of nucleic acid molecules
US6645739B2 (en) 2001-07-26 2003-11-11 Phoenix Pharmacologies, Inc. Yeast expression systems, methods of producing polypeptides in yeast, and compositions relating to same
WO2003089589A2 (en) * 2002-04-15 2003-10-30 Merck & Co., Inc. Matrix analysis of gene expression in cells (magec)
US7252933B2 (en) 2002-06-26 2007-08-07 Flanders Interuniversity Institute For Biotechnology Protein glycosylation modification in methylotrophic yeast
US20040204953A1 (en) * 2002-07-17 2004-10-14 Lincoln Muir Subscription based systems, methods and components for providing genomic and proteomic products and services
DE60335602D1 (de) * 2002-12-18 2011-02-17 Roche Diagnostics Gmbh Rekombinante Desoxyribonuclease I aus Rinder-Pankreas mit hoher spezifischer Aktivität
DE60319333D1 (de) * 2002-12-20 2008-04-10 Roche Diagnostics Gmbh Hitzelabile Desoxyribonuklease I-Varianten
US7332299B2 (en) 2003-02-20 2008-02-19 Glycofi, Inc. Endomannosidases in the modification of glycoproteins in eukaryotes
EP1460425A1 (en) * 2003-03-17 2004-09-22 Boehringer Mannheim Gmbh Deglycosylated enzymes for conjugates
ES2366830T3 (es) * 2003-08-25 2011-10-25 Funzyme Biotechnologies Sa Nuevas proteínas fúngicas y ácidos nucelicos que codifican las mismas.
US8293503B2 (en) 2003-10-03 2012-10-23 Promega Corporation Vectors for directional cloning
US8304189B2 (en) 2003-12-01 2012-11-06 Life Technologies Corporation Nucleic acid molecules containing recombination sites and methods of using the same
CA2486195C (en) * 2003-12-05 2012-03-06 Stephan Glaser Recombinantly expressed carboxypeptidase b and purification thereof
PT1926749E (pt) * 2005-09-14 2011-09-29 Sanofi Aventis Deutschland Clivagem de precursores de insulinas por uma variante de tripsina
AT504588B1 (de) * 2006-11-16 2008-08-15 Univ Graz Tech Nukleinsäure-promotor
EP2257638B1 (en) 2008-03-03 2014-12-31 AbbVie Inc. Methods for transforming yeast
JP5726088B2 (ja) 2008-12-12 2015-05-27 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ 再生可能資源から直鎖ジカルボン酸を製造する方法
EP2258855A1 (en) 2009-05-28 2010-12-08 Universität für Bodenkultur Wien Expression sequences
EP2515932A2 (en) 2009-12-21 2012-10-31 Centre Hospitalier Universitaire Vaudois (CHUV) Synergic action of a prolyl protease and tripeptidyl proteases
SI2710114T1 (sl) 2011-05-20 2022-01-31 H. Lundbeck A/S Proizvodnja visoke čistote proteinov z več podenotami, kot so protitelesa v transformiranih mikrobih, kot je Pichia pastoris
SG10201609752YA (en) 2011-08-19 2017-01-27 Alderbio Holdings Llc Multi-copy strategy for high-titer and high-purity production of multi-subunit proteins such as antibodies in transformed microbes such as pichia pastoris
ES2913825T3 (es) 2011-10-07 2022-06-06 Lonza Ag Promotor regulable
CA2903568C (en) 2013-03-15 2022-07-12 Lonza Ltd Constitutive promoter
EP3597664A3 (en) 2013-03-15 2020-03-11 Alder Biopharmaceuticals, Inc. Temperature shift for high yield expression of polypeptides in yeast and other transformed cells
US9150870B2 (en) 2013-03-15 2015-10-06 Lonza Ltd. Constitutive promoter
AR095615A1 (es) 2013-03-15 2015-10-28 Alder Biopharmaceuticals Inc Purificación de anticuerpos y monitoreo de pureza
CN105143243B (zh) 2013-03-21 2020-03-13 国家科学和工业研究组织 三螺旋蛋白的纯化
CA2910745A1 (en) 2013-04-30 2014-11-06 Donald Danforth Plant Science Center Antifungal plant proteins, peptides, and methods of use
TWI738631B (zh) 2013-09-09 2021-09-11 德商贏創運營有限公司 經修飾之細菌類膠原蛋白蛋白質
WO2016120764A1 (en) 2015-01-26 2016-08-04 Centre Hospitalier Universitaire Vaudois (Chuv) Aspergillus oryzae prolyl endopeptidases and use thereof in degradation of polypeptides
WO2021126129A1 (en) * 2019-12-19 2021-06-24 Atatürk Üni̇versi̇tesi̇ Bi̇li̇msel Araştirma Projeleri̇ Bi̇ri̇mi̇ A method for recombinant protein production

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE54046B1 (en) * 1981-08-25 1989-05-24 Celltech Ltd Expression vectors
IE54592B1 (en) * 1982-03-08 1989-12-06 Genentech Inc Anumal interferons, processes involved in their production, compositions containing them, dna sequences coding therefor and espression vehicles containing such sequences and cells transformed thereby
AR241796A1 (es) * 1982-05-19 1992-12-30 Gist Brocades Nv Procedimiento para preparar levadura klyveromyces que contienen vectores que regulan la expresion de proteina y/o capaces de la expresion de proteinas homologas o heterologas.
EP0118551A1 (en) * 1982-09-15 1984-09-19 Collaborative Research Inc. Production of interferon in yeast by use of invertase promoter
CA1341130C (en) * 1984-07-27 2000-10-31 Adrianus Marinus Ledeboer Use of oxidoreductases in bleaching and/or detergent compositions and their preparation by microorganisms engineered by recombinant dna technology
US4885242A (en) * 1984-10-30 1989-12-05 Phillips Petroleum Company Genes from pichia histidine pathway and uses thereof
US4879231A (en) * 1984-10-30 1989-11-07 Phillips Petroleum Company Transformation of yeasts of the genus pichia
US4837148A (en) * 1984-10-30 1989-06-06 Phillips Petroleum Company Autonomous replication sequences for yeast strains of the genus pichia

Also Published As

Publication number Publication date
JPS61173781A (ja) 1986-08-05
AR242988A1 (es) 1993-06-30
ATE83263T1 (de) 1992-12-15
JPH0698789A (ja) 1994-04-12
JP2502508B2 (ja) 1996-05-29
GR852611B (zh) 1986-03-04
FI94427C (fi) 1995-09-11
JPH06233686A (ja) 1994-08-23
DE3586889D1 (de) 1993-01-21
YU46695B (sh) 1994-04-05
JP2552807B2 (ja) 1996-11-13
US4855231A (en) 1989-08-08
DD254211A5 (de) 1988-02-17
AU572002B2 (en) 1988-04-28
ES548307A0 (es) 1986-09-01
HU205627B (en) 1992-05-28
DE3586889T2 (de) 1993-04-15
EP0483115A2 (en) 1992-04-29
PT81399A (en) 1985-11-01
NZ213842A (en) 1991-11-26
YU169585A (en) 1991-04-30
EP0483115A3 (en) 1992-05-06
ES8609458A1 (es) 1986-09-01
NO178076C (no) 1996-01-17
JPH08173161A (ja) 1996-07-09
FI94427B (fi) 1995-05-31
EP0183071A2 (en) 1986-06-04
PL158965B1 (pl) 1992-10-30
IE930804L (en) 1986-04-30
FI854142L (fi) 1986-05-01
EP0183071B1 (en) 1992-12-09
NO854312L (no) 1986-05-02
AU4875285A (en) 1986-06-12
FI854142A0 (fi) 1985-10-23
EP0183071A3 (en) 1987-09-23
KR930001117B1 (ko) 1993-02-18
NO178076B (no) 1995-10-09
CA1340733C (en) 1999-09-14
JPH08196275A (ja) 1996-08-06
CN1011243B (zh) 1991-01-16
IL76765A (en) 1991-06-30
PT81399B (pt) 1987-11-11
PL256012A1 (en) 1986-09-23
JP2552808B2 (ja) 1996-11-13
HUT38678A (en) 1986-06-30
CN85109718A (zh) 1986-08-20
KR870003197A (ko) 1987-04-15
DK496385D0 (da) 1985-10-29

Similar Documents

Publication Publication Date Title
DK496385D0 (da) Reguleringsregion for heterolog genekspression i gaer
ZA858181B (en) Regulatory region for heterologous gene expression in yeast
AU3824285A (en) Exogenous gene expression insect cells
NZ221790A (en) Method for the continuous production of a heterologous protein in a eukaryotic host cell
IT8547761A0 (it) Perfezionamento nelle lampade di il luminazione
EP0190254A4 (en) GENE CLONE AND PROCESS FOR THE PREPARATION AND USE THEREOF.
DK479685D0 (da) Stol
DK90985D0 (da) Fermenteringsstyreanlaeg
EP0129268A3 (en) Improvements in the expression of newly introduced genes in yeast cells
DK179785A (da) Fermenteringsproces
IT1185032B (it) Riscaldatore di combustibile
DK535485D0 (da) Indstillelig stol
EP0252737A3 (en) Improved strains of yeast for the expression of heterologous genes
DK246585D0 (da) Flercellet kraftforsyning
DE3584424D1 (de) Geregelte hochspannungsversorgung.
EP0163472A3 (en) Medium for electrophoresis
PT79046B (en) Yeast copper-metallothionein gene
IL83435A0 (en) Gene expression in yeast
ZA846096B (en) Yeast copper-metallothionein gene
KR860002144U (ko) 배양액 순환 재배조
AT385573B (de) Gesteuerter durchflussregler
GB8418511D0 (en) Regulation of gene expression
GB8423301D0 (en) Regulation of gene expression
IT1177911B (it) Perfezionamenti nelle serre
FI844657A0 (fi) Elektrisk bastuugn med i sten- eller annat vaermelagrande material slutna motstaond eller motstaondselement.

Legal Events

Date Code Title Description
AHB Application shelved due to non-payment