DK3272860T3 - Pyruvatdehydrogenase-mutant, mikroorganisme, der omfatter mutanten, og fremgangsmåde til fremstilling af L-aminosyre ved anvendelse af mikroorganismen - Google Patents

Pyruvatdehydrogenase-mutant, mikroorganisme, der omfatter mutanten, og fremgangsmåde til fremstilling af L-aminosyre ved anvendelse af mikroorganismen Download PDF

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DK3272860T3
DK3272860T3 DK16765246.0T DK16765246T DK3272860T3 DK 3272860 T3 DK3272860 T3 DK 3272860T3 DK 16765246 T DK16765246 T DK 16765246T DK 3272860 T3 DK3272860 T3 DK 3272860T3
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microorganism
mutant
producing
amino acid
pyruvate dehydrogenase
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DK16765246.0T
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Lan Huh
Jun Ok Moon
Hyun Won Bae
Hyung Joon Kim
Sung Ki Song
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Cj Cheiljedang Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01051—Pyruvate dehydrogenase (NADP+) (1.2.1.51)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/04—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with a disulfide as acceptor (1.2.4)
    • C12Y102/04001—Pyruvate dehydrogenase (acetyl-transferring) (1.2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/15—Corynebacterium

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
DK16765246.0T 2015-03-18 2016-03-15 Pyruvatdehydrogenase-mutant, mikroorganisme, der omfatter mutanten, og fremgangsmåde til fremstilling af L-aminosyre ved anvendelse af mikroorganismen DK3272860T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020150037654A KR101776375B1 (ko) 2015-03-18 2015-03-18 피루브산 디하이드로게나아제 변이체, 이를 포함하는 미생물 및 이를 이용한 l-아미노산 생산 방법
PCT/KR2016/002594 WO2016148490A1 (ko) 2015-03-18 2016-03-15 피루브산 디하이드로게나아제 변이체, 이를 포함하는 미생물 및 이를 이용한 l-아미노산 생산 방법

Publications (1)

Publication Number Publication Date
DK3272860T3 true DK3272860T3 (da) 2023-10-30

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ID=56919058

Family Applications (1)

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DK16765246.0T DK3272860T3 (da) 2015-03-18 2016-03-15 Pyruvatdehydrogenase-mutant, mikroorganisme, der omfatter mutanten, og fremgangsmåde til fremstilling af L-aminosyre ved anvendelse af mikroorganismen

Country Status (13)

Country Link
US (2) US10526586B2 (da)
EP (1) EP3272860B1 (da)
JP (2) JP6625133B2 (da)
KR (1) KR101776375B1 (da)
CN (1) CN106715687B (da)
BR (1) BR112017019057B1 (da)
DK (1) DK3272860T3 (da)
ES (1) ES2959213T3 (da)
HU (1) HUE064139T2 (da)
MY (1) MY187636A (da)
PL (1) PL3272860T3 (da)
RU (1) RU2683208C1 (da)
WO (1) WO2016148490A1 (da)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101947945B1 (ko) * 2018-01-25 2019-02-13 씨제이제일제당 (주) L-아미노산을 생산하는 코리네박테리움 속 미생물 및 이를 이용한 l-아미노산의 생산방법
KR101947959B1 (ko) * 2018-05-28 2019-02-13 씨제이제일제당 (주) 변이형 호모세린 디하이드로게나제 및 이를 이용한 호모세린 또는 호모세린 유래 l-아미노산의 생산 방법
KR102311391B1 (ko) 2020-05-21 2021-10-12 씨제이제일제당 주식회사 L- 분지쇄 아미노산 생산능이 강화된 미생물 및 이를 이용하여 l-분지쇄 아미노산을 생산하는 방법
KR102344689B1 (ko) * 2020-09-01 2021-12-29 씨제이제일제당 주식회사 L-발린 생산 미생물 및 이를 이용한 l-발린 생산 방법
US20220372451A1 (en) * 2021-04-28 2022-11-24 University Of Georgia Research Foundation, Inc. Production of pyruvate or products derived from pyruvate using modified escherichia coli
CN113249347B (zh) * 2021-07-14 2021-11-12 中国科学院天津工业生物技术研究所 丙酮酸脱氢酶的突变体及其用于生产l-氨基酸的方法
CN113430151B (zh) * 2021-08-13 2022-06-07 江苏澳创生物科技有限公司 一株谷氨酸棒杆菌及其在发酵生产l-缬氨酸中的应用
CN113683667A (zh) * 2021-08-25 2021-11-23 宁夏伊品生物科技股份有限公司 Yh66-rs10865基因改造得到的工程菌及其在制备缬氨酸中的应用
KR20230108790A (ko) * 2022-01-11 2023-07-19 대상 주식회사 L-히스티딘 생산능이 향상된 에스케리치아 속 변이주 및 이를 이용한 l-히스티딘의 생산 방법

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR940001307B1 (ko) 1991-12-27 1994-02-19 제일제당 주식회사 L-라이신을 생산하는 신규 미생물
ATE483792T1 (de) * 1998-12-18 2010-10-15 Ajinomoto Kk Verfahren zur fermentativen herstellung von l- glutaminsäure
TW200734459A (en) * 1999-10-04 2007-09-16 Ajinomoto Kk Genes for heat resistant enzymes of amino acid biosynthetic pathway derived from thermophilic coryneform bacteria
DE102005045301A1 (de) 2005-09-22 2007-04-05 Degussa Ag Verfahren zur Herstellung von organisch-chemischen Verbindungen unter Verwendung coryneformer Bakterien
DE102006032634A1 (de) 2006-07-13 2008-01-17 Evonik Degussa Gmbh Verfahren zur Herstellung von L-Aminosäuren
EP3170889A1 (en) 2006-09-15 2017-05-24 CJ Cheiljedang Corporation A corynebacteria having enhanced l-lysine productivity and a method of producing l-lysine using the same
RU2496867C2 (ru) * 2011-04-25 2013-10-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО "АГРИ") Способ получения l-аминокислоты семейства глутамата с использованием коринеформной бактерии
US9121038B2 (en) * 2011-04-29 2015-09-01 The Goodyear Tire & Rubber Company Recombinant microorganisms for enhanced production of mevalonate, isoprene, and isoprenoids
KR101117022B1 (ko) 2011-08-16 2012-03-16 씨제이제일제당 (주) Ｌ-발린 생산능이 향상된 미생물 및 이를 이용한 ｌ-발린 제조방법
KR101721722B1 (ko) 2013-03-11 2017-04-10 씨제이제일제당 (주) L-발린 생산능이 향상된 균주 및 이를 이용한 l-발린 생산방법
CN104004678A (zh) * 2014-05-05 2014-08-27 江南大学 一株高产l-缬氨酸的谷氨酸棒状杆菌工程菌的构建及其发酵产l-缬氨酸的方法

Also Published As

Publication number Publication date
EP3272860B1 (en) 2023-08-09
US10526586B2 (en) 2020-01-07
PL3272860T3 (pl) 2024-02-05
CN106715687A (zh) 2017-05-24
JP2018512132A (ja) 2018-05-17
ES2959213T3 (es) 2024-02-21
RU2683208C1 (ru) 2019-03-26
EP3272860A4 (en) 2018-08-08
MY187636A (en) 2021-10-05
KR101776375B1 (ko) 2017-09-08
US20190292526A1 (en) 2019-09-26
BR112017019057A2 (pt) 2018-04-17
HUE064139T2 (hu) 2024-03-28
CN106715687B (zh) 2021-04-27
JP2020000250A (ja) 2020-01-09
KR20160113377A (ko) 2016-09-29
US20180057798A1 (en) 2018-03-01
KR101776375B9 (ko) 2022-01-11
WO2016148490A1 (ko) 2016-09-22
JP6625133B2 (ja) 2019-12-25
EP3272860A1 (en) 2018-01-24
BR112017019057B1 (pt) 2024-03-05

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