PT3161144T - Processo para a redução biocatalítica de células inteiras de compostos de ácido desidrocólico, assim como de mutantes de 7beta-hidroxiesteroide desidrogenase - Google Patents
Processo para a redução biocatalítica de células inteiras de compostos de ácido desidrocólico, assim como de mutantes de 7beta-hidroxiesteroide desidrogenaseInfo
- Publication number
- PT3161144T PT3161144T PT157319021T PT15731902T PT3161144T PT 3161144 T PT3161144 T PT 3161144T PT 157319021 T PT157319021 T PT 157319021T PT 15731902 T PT15731902 T PT 15731902T PT 3161144 T PT3161144 T PT 3161144T
- Authority
- PT
- Portugal
- Prior art keywords
- 7beta
- biocatalytic
- acid compounds
- whole cell
- hydroxysteroid dehydrogenase
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P33/00—Preparation of steroids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
- C12Y101/0105—3Alpha-hydroxysteroid 3-dehydrogenase (B-specific) (1.1.1.50)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
- C12Y101/01145—3-Beta-hydroxy-DELTA5-steroid dehydrogenase (1.1.1.145)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
- C12Y101/01047—Glucose 1-dehydrogenase (1.1.1.47)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
- C12Y101/01201—7-Beta-hydroxysteroid dehydrogenase (NADP+) (1.1.1.201)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Steroid Compounds (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP14173747 | 2014-06-24 | ||
EP14182290 | 2014-08-26 |
Publications (1)
Publication Number | Publication Date |
---|---|
PT3161144T true PT3161144T (pt) | 2019-12-18 |
Family
ID=53489945
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PT157319021T PT3161144T (pt) | 2014-06-24 | 2015-06-24 | Processo para a redução biocatalítica de células inteiras de compostos de ácido desidrocólico, assim como de mutantes de 7beta-hidroxiesteroide desidrogenase |
Country Status (7)
Country | Link |
---|---|
US (1) | US10544441B2 (pt) |
EP (1) | EP3161144B1 (pt) |
JP (1) | JP6666855B2 (pt) |
KR (1) | KR102640531B1 (pt) |
NZ (1) | NZ725965A (pt) |
PT (1) | PT3161144T (pt) |
WO (1) | WO2015197698A2 (pt) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP7035034B2 (ja) | 2016-06-20 | 2022-03-14 | ファルマツェル、ゲーエムベーハー | ケノデオキシコール酸のウルソデオキシコール酸への共役自己完結型(self-sufficient)生体内変換および前記方法において適用可能な新規酵素突然変異体 |
WO2018036982A1 (de) | 2016-08-22 | 2018-03-01 | Pharmazell Gmbh | Chemisch-biokatalytisches verfahren zur herstellung von ursodesoxycholsäure |
CN107980060B (zh) * | 2017-01-09 | 2021-05-25 | 深圳市邦泰绿色生物合成研究院 | 一种3α-羟基-7氧代-5β-胆烷酸的制备方法及其制备用酶2 |
NL2020823B1 (en) * | 2018-04-25 | 2019-11-05 | Univ Delft Tech | NAD+ dependent 7ß-hydroxysteroid dehydrogenase |
CN110387360B (zh) * | 2019-06-18 | 2021-12-28 | 华东理工大学 | 羟基类固醇脱氢酶及其在合成熊去氧胆酸前体中的应用 |
CN113968891B (zh) * | 2021-11-15 | 2023-04-07 | 湖南科瑞生物制药股份有限公司 | 一种植物源7-酮基石胆酸的制备方法 |
CN115386557A (zh) * | 2022-10-14 | 2022-11-25 | 安徽大学 | 一种7α-羟基类固醇脱氢酶的制备方法及其催化转化应用 |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5651997A (en) * | 1979-10-05 | 1981-05-09 | Toyobo Co Ltd | Preparation of 12-keto cheno deoxycholic acid by microorganism |
US6675479B1 (en) | 2000-02-29 | 2004-01-13 | The Gillette Company | Shaving razor and blade unit with improved guard |
EP2105500A1 (de) | 2008-03-26 | 2009-09-30 | Pharmazell GmbH | Neue 12alpha-Hydroxysteroiddehydrogenasen, deren Herstellung und deren Verwendung |
WO2011064404A1 (de) * | 2009-11-30 | 2011-06-03 | Pharmazell Gmbh | NEUARTIGE 7β-HYDROXYSTEROID DEHYDROGENASEN UND DEREN VERWENDUNG |
KR102023208B1 (ko) * | 2010-12-16 | 2019-09-19 | 파마젤 게엠베하 | 신규한 7β-히드록시스테로이드 데히드로게나제 돌연변이체 및 우르소데옥시콜산의 제조 방법 |
-
2015
- 2015-06-24 KR KR1020177001891A patent/KR102640531B1/ko active IP Right Grant
- 2015-06-24 NZ NZ725965A patent/NZ725965A/en unknown
- 2015-06-24 JP JP2016569760A patent/JP6666855B2/ja active Active
- 2015-06-24 WO PCT/EP2015/064264 patent/WO2015197698A2/de active Application Filing
- 2015-06-24 EP EP15731902.1A patent/EP3161144B1/de active Active
- 2015-06-24 US US15/314,155 patent/US10544441B2/en active Active
- 2015-06-24 PT PT157319021T patent/PT3161144T/pt unknown
Also Published As
Publication number | Publication date |
---|---|
JP2017522006A (ja) | 2017-08-10 |
US10544441B2 (en) | 2020-01-28 |
WO2015197698A3 (de) | 2016-02-25 |
EP3161144B1 (de) | 2019-09-18 |
NZ725965A (en) | 2022-04-29 |
EP3161144A2 (de) | 2017-05-03 |
JP6666855B2 (ja) | 2020-03-18 |
KR102640531B1 (ko) | 2024-02-23 |
KR20170020511A (ko) | 2017-02-22 |
WO2015197698A2 (de) | 2015-12-30 |
US20170191104A1 (en) | 2017-07-06 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP3432320A4 (en) | SOLID ELECTROLYTE, AND METHOD OF MANUFACTURING THE SAME | |
EP3332583A4 (en) | Method of performing cell selection and re-selection using pmax parameters and system adapted thereto | |
EP3231024A4 (en) | Electrochemical cell and method of making the same | |
PT3444340T (pt) | Mutantes da 7β-hidroxiesteroide desidrogenase e processos para a produção do ácido ursodesoxicólico | |
EP3164899A4 (en) | Multi-electrode electrochemical cell and method of making the same | |
PT3161144T (pt) | Processo para a redução biocatalítica de células inteiras de compostos de ácido desidrocólico, assim como de mutantes de 7beta-hidroxiesteroide desidrogenase | |
SG11201701356WA (en) | Solar cell and method for producing thereof | |
EP3101723A4 (en) | Secondary battery and secondary battery production method | |
EP3124589A4 (en) | Cell culture method and cell culture system | |
EP3161646A4 (en) | System and method for indentification of non-human users acessing content | |
EP3223831A4 (en) | Methods for generation of podocytes from pluripotent stem cells and cells produced by the same | |
EP3232494A4 (en) | Separator for secondary cell, method for manufacturing separator for secondary cell, and secondary cell | |
EP3157062A4 (en) | Solar cell and method for manufacturing solar cell | |
EP3225685A4 (en) | Cell preservative solution, use of same, and method for producing cell preservative solution | |
EP3093904A4 (en) | Separator for batteries and method for producing same | |
ZA201601951B (en) | Method for obtaining low ethanol-producing yeast strains, yeast strains obtained therefrom and their use | |
EP3139432A4 (en) | Fuel cell system and method for controlling same | |
PT3180424T (pt) | 3alfa-hidroxisteroide desidrogenase mutantes e processo para a produção de ácido ursodesoxicólico | |
EP3220464A4 (en) | Cell structure, method for producing same, and fuel cell | |
EP3167491A4 (en) | Compound-semiconductor photovoltaic cell and manufacturing method of compound-semiconductor photovoltaic cell | |
EP3279990A4 (en) | Separator for fuel cells and method for producing same | |
EP3170893A4 (en) | Method for extracting differentiated cells | |
EP3496162A4 (en) | SOLAR CELL AND METHOD FOR MANUFACTURING SAME | |
EP3255147A4 (en) | Immobilized cell and preparation method thereof | |
EP3156080A4 (en) | Cell structure and method for producing cell structure |