PT3161144T - Processo para a redução biocatalítica de células inteiras de compostos de ácido desidrocólico, assim como de mutantes de 7beta-hidroxiesteroide desidrogenase - Google Patents

Processo para a redução biocatalítica de células inteiras de compostos de ácido desidrocólico, assim como de mutantes de 7beta-hidroxiesteroide desidrogenase

Info

Publication number
PT3161144T
PT3161144T PT157319021T PT15731902T PT3161144T PT 3161144 T PT3161144 T PT 3161144T PT 157319021 T PT157319021 T PT 157319021T PT 15731902 T PT15731902 T PT 15731902T PT 3161144 T PT3161144 T PT 3161144T
Authority
PT
Portugal
Prior art keywords
7beta
biocatalytic
acid compounds
whole cell
hydroxysteroid dehydrogenase
Prior art date
Application number
PT157319021T
Other languages
English (en)
Inventor
Weuster-Botz Dirk
Sun Boqiao
Quirin Christian
Original Assignee
Pharmazell Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pharmazell Gmbh filed Critical Pharmazell Gmbh
Publication of PT3161144T publication Critical patent/PT3161144T/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/0105—3Alpha-hydroxysteroid 3-dehydrogenase (B-specific) (1.1.1.50)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01145—3-Beta-hydroxy-DELTA5-steroid dehydrogenase (1.1.1.145)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01047—Glucose 1-dehydrogenase (1.1.1.47)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01201—7-Beta-hydroxysteroid dehydrogenase (NADP+) (1.1.1.201)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Steroid Compounds (AREA)
PT157319021T 2014-06-24 2015-06-24 Processo para a redução biocatalítica de células inteiras de compostos de ácido desidrocólico, assim como de mutantes de 7beta-hidroxiesteroide desidrogenase PT3161144T (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP14173747 2014-06-24
EP14182290 2014-08-26

Publications (1)

Publication Number Publication Date
PT3161144T true PT3161144T (pt) 2019-12-18

Family

ID=53489945

Family Applications (1)

Application Number Title Priority Date Filing Date
PT157319021T PT3161144T (pt) 2014-06-24 2015-06-24 Processo para a redução biocatalítica de células inteiras de compostos de ácido desidrocólico, assim como de mutantes de 7beta-hidroxiesteroide desidrogenase

Country Status (7)

Country Link
US (1) US10544441B2 (pt)
EP (1) EP3161144B1 (pt)
JP (1) JP6666855B2 (pt)
KR (1) KR102640531B1 (pt)
NZ (1) NZ725965A (pt)
PT (1) PT3161144T (pt)
WO (1) WO2015197698A2 (pt)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7035034B2 (ja) 2016-06-20 2022-03-14 ファルマツェル、ゲーエムベーハー ケノデオキシコール酸のウルソデオキシコール酸への共役自己完結型（ｓｅｌｆ－ｓｕｆｆｉｃｉｅｎｔ）生体内変換および前記方法において適用可能な新規酵素突然変異体
WO2018036982A1 (de) 2016-08-22 2018-03-01 Pharmazell Gmbh Chemisch-biokatalytisches verfahren zur herstellung von ursodesoxycholsäure
CN107980060B (zh) * 2017-01-09 2021-05-25 深圳市邦泰绿色生物合成研究院 一种3α-羟基-7氧代-5β-胆烷酸的制备方法及其制备用酶2
NL2020823B1 (en) * 2018-04-25 2019-11-05 Univ Delft Tech NAD+ dependent 7ß-hydroxysteroid dehydrogenase
CN110387360B (zh) * 2019-06-18 2021-12-28 华东理工大学 羟基类固醇脱氢酶及其在合成熊去氧胆酸前体中的应用
CN113968891B (zh) * 2021-11-15 2023-04-07 湖南科瑞生物制药股份有限公司 一种植物源7-酮基石胆酸的制备方法
CN115386557A (zh) * 2022-10-14 2022-11-25 安徽大学 一种7α-羟基类固醇脱氢酶的制备方法及其催化转化应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5651997A (en) * 1979-10-05 1981-05-09 Toyobo Co Ltd Preparation of 12-keto cheno deoxycholic acid by microorganism
US6675479B1 (en) 2000-02-29 2004-01-13 The Gillette Company Shaving razor and blade unit with improved guard
EP2105500A1 (de) 2008-03-26 2009-09-30 Pharmazell GmbH Neue 12alpha-Hydroxysteroiddehydrogenasen, deren Herstellung und deren Verwendung
WO2011064404A1 (de) * 2009-11-30 2011-06-03 Pharmazell Gmbh NEUARTIGE 7β-HYDROXYSTEROID DEHYDROGENASEN UND DEREN VERWENDUNG
KR102023208B1 (ko) * 2010-12-16 2019-09-19 파마젤 게엠베하 신규한 7β-히드록시스테로이드 데히드로게나제 돌연변이체 및 우르소데옥시콜산의 제조 방법

Also Published As

Publication number Publication date
JP2017522006A (ja) 2017-08-10
US10544441B2 (en) 2020-01-28
WO2015197698A3 (de) 2016-02-25
EP3161144B1 (de) 2019-09-18
NZ725965A (en) 2022-04-29
EP3161144A2 (de) 2017-05-03
JP6666855B2 (ja) 2020-03-18
KR102640531B1 (ko) 2024-02-23
KR20170020511A (ko) 2017-02-22
WO2015197698A2 (de) 2015-12-30
US20170191104A1 (en) 2017-07-06

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