PT3180424T - 3alfa-hidroxisteroide desidrogenase mutantes e processo para a produção de ácido ursodesoxicólico - Google Patents

3alfa-hidroxisteroide desidrogenase mutantes e processo para a produção de ácido ursodesoxicólico

Info

Publication number
PT3180424T
PT3180424T PT157507047T PT15750704T PT3180424T PT 3180424 T PT3180424 T PT 3180424T PT 157507047 T PT157507047 T PT 157507047T PT 15750704 T PT15750704 T PT 15750704T PT 3180424 T PT3180424 T PT 3180424T
Authority
PT
Portugal
Prior art keywords
alpha
preparation
hydroxysteroid dehydrogenase
ursodeoxycholic acid
dehydrogenase mutants
Prior art date
Application number
PT157507047T
Other languages
English (en)
Original Assignee
Pharmazell Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pharmazell Gmbh filed Critical Pharmazell Gmbh
Publication of PT3180424T publication Critical patent/PT3180424T/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/02—Dehydrogenating; Dehydroxylating
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/0105—3Alpha-hydroxysteroid 3-dehydrogenase (B-specific) (1.1.1.50)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01159—7-Alpha-hydroxysteroid dehydrogenase (1.1.1.159)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01225—Chlordecone reductase (1.1.1.225)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
PT157507047T 2014-08-12 2015-08-12 3alfa-hidroxisteroide desidrogenase mutantes e processo para a produção de ácido ursodesoxicólico PT3180424T (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP14180614 2014-08-12

Publications (1)

Publication Number Publication Date
PT3180424T true PT3180424T (pt) 2020-06-05

Family

ID=51300642

Family Applications (1)

Application Number Title Priority Date Filing Date
PT157507047T PT3180424T (pt) 2014-08-12 2015-08-12 3alfa-hidroxisteroide desidrogenase mutantes e processo para a produção de ácido ursodesoxicólico

Country Status (6)

Country Link
US (2) US10487348B2 (pt)
EP (1) EP3180424B1 (pt)
JP (1) JP6694871B2 (pt)
KR (1) KR102471639B1 (pt)
PT (1) PT3180424T (pt)
WO (1) WO2016023933A1 (pt)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018036982A1 (de) 2016-08-22 2018-03-01 Pharmazell Gmbh Chemisch-biokatalytisches verfahren zur herstellung von ursodesoxycholsäure
CN107841489B (zh) * 2017-11-14 2020-02-18 重庆大学 撒丁岛梭菌7α-羟基类固醇脱氢酶突变体K179M
CN111235122B (zh) * 2019-01-29 2020-10-30 武汉生之源生物科技股份有限公司 一种3α羟类固醇脱氢酶突变体及其在总胆汁酸检测中的应用
CN110387360B (zh) * 2019-06-18 2021-12-28 华东理工大学 羟基类固醇脱氢酶及其在合成熊去氧胆酸前体中的应用
CN111676202B (zh) * 2020-08-05 2023-04-25 重庆极泽生物科技有限公司 一种共表达羟基类固醇脱氢酶的发酵工艺
WO2022169909A2 (en) * 2021-02-02 2022-08-11 Novome Biotechnologies, Inc. Methods and compositions for treating disorders associated with bile acids
CN113151207B (zh) * 2021-04-21 2023-03-31 重庆第二师范学院 NAD(H)依赖型3α-羟基类固醇脱氢酶及其编码基因
CN114854707B (zh) * 2022-06-14 2023-09-12 苏州百福安酶技术有限公司 一种7β-羟基甾体脱氢酶突变体

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101387240B1 (ko) * 2005-06-20 2014-04-21 아크레이 가부시키가이샤 변이 글루코오스 탈수소효소
JP2013511973A (ja) * 2009-11-30 2013-04-11 ファルマツェル、ゲーエムベーハー 新規７β−ヒドロキシステロイドデヒドロゲナーゼおよびその使用
JP6023045B2 (ja) 2010-05-27 2016-11-09 ファルマツェル、ゲーエムベーハー 新規７α−ヒドロキシステロイドデヒドロゲナーゼノックアウト突然変異体およびその使用
US20140087421A1 (en) 2010-12-16 2014-03-27 Pharmazell Gmbh NOVEL 7Beta-HYDROXYSTEROID DEHYDROGENASE MUTANTS AND PROCESS FOR THE PREPARATION OF URSODEOXYCHOLIC ACID

Also Published As

Publication number Publication date
US20170306382A1 (en) 2017-10-26
US11306343B2 (en) 2022-04-19
EP3180424A1 (de) 2017-06-21
NZ728430A (en) 2022-03-25
WO2016023933A1 (de) 2016-02-18
JP6694871B2 (ja) 2020-05-20
KR102471639B1 (ko) 2022-11-25
US10487348B2 (en) 2019-11-26
JP2017523792A (ja) 2017-08-24
EP3180424B1 (de) 2020-03-18
US20190390242A1 (en) 2019-12-26
KR20170036797A (ko) 2017-04-03

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