PT3174977T - Mutantes da 7 -hidroxiesteroide desidrogenase e processos para a produção do ácido ursodesoxicólico - Google Patents

Mutantes da 7 -hidroxiesteroide desidrogenase e processos para a produção do ácido ursodesoxicólico

Info

Publication number
PT3174977T
PT3174977T PT15741571T PT15741571T PT3174977T PT 3174977 T PT3174977 T PT 3174977T PT 15741571 T PT15741571 T PT 15741571T PT 15741571 T PT15741571 T PT 15741571T PT 3174977 T PT3174977 T PT 3174977T
Authority
PT
Portugal
Prior art keywords
7beta
hydroxysteroid dehydrogenase
ursodeoxycholic acid
dehydrogenase mutants
producing ursodeoxycholic
Prior art date
Application number
PT15741571T
Other languages
English (en)
Inventor
Hummel Werner
Bakonyi Daniel
Original Assignee
Pharmazell Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pharmazell Gmbh filed Critical Pharmazell Gmbh
Publication of PT3174977T publication Critical patent/PT3174977T/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/02—Dehydrogenating; Dehydroxylating
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01201—7-Beta-hydroxysteroid dehydrogenase (NADP+) (1.1.1.201)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
PT15741571T 2014-07-29 2015-07-28 Mutantes da 7 -hidroxiesteroide desidrogenase e processos para a produção do ácido ursodesoxicólico PT3174977T (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP14178912 2014-07-29

Publications (1)

Publication Number Publication Date
PT3174977T true PT3174977T (pt) 2019-07-16

Family

ID=51225414

Family Applications (2)

Application Number Title Priority Date Filing Date
PT15741571T PT3174977T (pt) 2014-07-29 2015-07-28 Mutantes da 7 -hidroxiesteroide desidrogenase e processos para a produção do ácido ursodesoxicólico
PT181966169T PT3444340T (pt) 2014-07-29 2015-07-28 Mutantes da 7β-hidroxiesteroide desidrogenase e processos para a produção do ácido ursodesoxicólico

Family Applications After (1)

Application Number Title Priority Date Filing Date
PT181966169T PT3444340T (pt) 2014-07-29 2015-07-28 Mutantes da 7β-hidroxiesteroide desidrogenase e processos para a produção do ácido ursodesoxicólico

Country Status (7)

Country Link
US (3) US10358672B2 (pt)
EP (2) EP3174977B1 (pt)
JP (1) JP6582040B2 (pt)
KR (1) KR102394701B1 (pt)
NZ (2) NZ765044A (pt)
PT (2) PT3174977T (pt)
WO (1) WO2016016213A1 (pt)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3472339B1 (en) 2016-06-20 2020-09-02 Pharmazell GmbH Coupled biotransformation of chenodeoxycholic acid to ursodeoxycholic acid and enzyme mutants applicable in said process
WO2018036982A1 (de) 2016-08-22 2018-03-01 Pharmazell Gmbh Chemisch-biokatalytisches verfahren zur herstellung von ursodesoxycholsäure
CN107841489B (zh) * 2017-11-14 2020-02-18 重庆大学 撒丁岛梭菌7α-羟基类固醇脱氢酶突变体K179M
NL2020823B1 (en) * 2018-04-25 2019-11-05 Univ Delft Tech NAD+ dependent 7ß-hydroxysteroid dehydrogenase
KR20200069649A (ko) 2018-12-07 2020-06-17 박찬우 상변화 물질을 이용한 휴대폰 발열 줄이기
CN109929896B (zh) * 2019-04-23 2022-06-14 南京久安源环保科技有限公司 一种熊去氧胆酸的生产工艺
IT201900017411A1 (it) * 2019-09-27 2021-03-27 Ice S P A Processo di preparazione di acido ursodesossicolico
EP3933036A1 (en) * 2020-07-02 2022-01-05 Herbrand PharmaChemicals GmbH Process for 7-beta-hydroxylation of bile acid derivatives
CN114540338B (zh) * 2020-11-25 2023-09-05 湖南引航生物科技有限公司 固定化经修饰的7β-羟基甾体脱氢酶及其应用
JP2023552528A (ja) * 2020-11-30 2023-12-18 サンドヒル ワン，エルエルシー Ｌｃａおよび３－ｋｃａをｕｄｃａおよび３－ｋｕｄｃａに変換するための酵素的方法
CN113388592B (zh) * 2021-06-30 2022-06-24 中山百灵生物技术股份有限公司 一种7β-HSDH酶突变体及其编码基因和应用
CN113462665B (zh) * 2021-06-30 2023-03-10 中山百灵生物技术股份有限公司 一种7α-HSDH酶突变体及其编码基因和应用
KR20230058876A (ko) 2021-10-25 2023-05-03 에이스바이오팜 주식회사 7-Keto-LCA에서 우르소데옥시콜산으로의 전환율을 증진시키는 루미노코쿠스 나버스 균주 유래 7-beta-HSDH의 변이체 및 이를 이용한 우르소데옥시콜산 생산방법

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19931847A1 (de) 1999-07-09 2001-01-11 Basf Ag Immobilisierte Lipase
DE10019380A1 (de) 2000-04-19 2001-10-25 Basf Ag Verfahren zur Herstellung von kovalent gebundenen biologisch aktiven Stoffen an Polyurethanschaumstoffen sowie Verwendung der geträgerten Polyurethanschaumstoffe für chirale Synthesen
DE10019377A1 (de) 2000-04-19 2001-10-25 Basf Ag Verfahren zur Immobilisierung von biologisch aktiven Stoffen auf Trägermaterialien und Verwendung der mit biologisch aktiven Stoffen geträgerten Materialien für chirale Synthesen
JP2013511973A (ja) * 2009-11-30 2013-04-11 ファルマツェル、ゲーエムベーハー 新規７β−ヒドロキシステロイドデヒドロゲナーゼおよびその使用
AU2011257168B2 (en) 2010-05-27 2015-03-19 Pharmazell Gmbh Novel 7alpha-hydroxysteroid dehydrogenase knockout mutants and use thereof
CN105441399A (zh) 2010-12-16 2016-03-30 细胞制药有限公司 新的7β-羟基类固醇脱氢酶突变体和制备熊去氧胆酸的方法

Also Published As

Publication number Publication date
JP2017522034A (ja) 2017-08-10
EP3444340B1 (de) 2020-04-15
WO2016016213A1 (de) 2016-02-04
US11198896B2 (en) 2021-12-14
NZ727413A (en) 2022-04-29
US10724064B2 (en) 2020-07-28
EP3174977A1 (de) 2017-06-07
US20200407767A1 (en) 2020-12-31
US20170226556A1 (en) 2017-08-10
KR20170036693A (ko) 2017-04-03
US10358672B2 (en) 2019-07-23
EP3174977B1 (de) 2019-04-10
NZ765044A (en) 2022-04-29
JP6582040B2 (ja) 2019-09-25
KR102394701B1 (ko) 2022-05-04
PT3444340T (pt) 2020-06-16
US20190338333A1 (en) 2019-11-07
EP3444340A1 (de) 2019-02-20

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