KR101776375B9 - 피루브산 디하이드로게나아제 변이체, 이를 포함하는 미생물 및 이를 이용한 l-아미노산 생산 방법 - Google Patents
피루브산 디하이드로게나아제 변이체, 이를 포함하는 미생물 및 이를 이용한 l-아미노산 생산 방법Info
- Publication number
- KR101776375B9 KR101776375B9 KR1020150037654A KR20150037654A KR101776375B9 KR 101776375 B9 KR101776375 B9 KR 101776375B9 KR 1020150037654 A KR1020150037654 A KR 1020150037654A KR 20150037654 A KR20150037654 A KR 20150037654A KR 101776375 B9 KR101776375 B9 KR 101776375B9
- Authority
- KR
- South Korea
- Prior art keywords
- same
- microorganism
- producing
- amino acid
- pyruvate dehydrogenase
- Prior art date
Links
- 101710088194 Dehydrogenase Proteins 0.000 title 1
- 150000008575 L-amino acids Chemical class 0.000 title 1
- 238000004519 manufacturing process Methods 0.000 title 1
- 244000005700 microbiome Species 0.000 title 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
- C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
- C12Y102/01051—Pyruvate dehydrogenase (NADP+) (1.2.1.51)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
- C12Y102/04—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with a disulfide as acceptor (1.2.4)
- C12Y102/04001—Pyruvate dehydrogenase (acetyl-transferring) (1.2.4.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/15—Corynebacterium
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Priority Applications (15)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020150037654A KR101776375B1 (ko) | 2015-03-18 | 2015-03-18 | 피루브산 디하이드로게나아제 변이체, 이를 포함하는 미생물 및 이를 이용한 l-아미노산 생산 방법 |
| PCT/KR2016/002594 WO2016148490A1 (ko) | 2015-03-18 | 2016-03-15 | 피루브산 디하이드로게나아제 변이체, 이를 포함하는 미생물 및 이를 이용한 l-아미노산 생산 방법 |
| MYPI2017703025A MY187636A (en) | 2015-03-18 | 2016-03-15 | Pyruvate dehydrogenase variants, a microorganism comprising the same and a method for producing l-amino acid using the same |
| BR112017019057-5A BR112017019057B1 (pt) | 2015-03-18 | 2016-03-15 | Variantes de piruvato desidrogenase, um micro-organismo que compreende as mesmas e um método para produzir l-aminoácido com o uso das mesmas |
| PL16765246.0T PL3272860T3 (pl) | 2015-03-18 | 2016-03-15 | Mutant dehydrogenazy pirogronianowej, mikroorganizm zawierający mutanta oraz sposób wytwarzania l-aminokwasu przy zastosowaniu mikroorganizmu |
| ES16765246T ES2959213T3 (es) | 2015-03-18 | 2016-03-15 | Mutante de piruvato deshidrogenasa, microorganismo que comprende el mutante y método para producir l-aminoácido usando el microorganismo |
| DK16765246.0T DK3272860T5 (da) | 2015-03-18 | 2016-03-15 | Pyruvatdehydrogenase-mutant, mikroorganisme, der omfatter mutanten, og fremgangsmåde til fremstilling af L-aminosyre ved anvendelse af mikroorganismen |
| HUE16765246A HUE064139T2 (hu) | 2015-03-18 | 2016-03-15 | Piruvát-dehidrogenáz-mutáns, mutánst tartalmazó mikroorganizmus, és eljárás L-aminósav elõállítására mikroorganizmus használatával |
| JP2017548259A JP6625133B2 (ja) | 2015-03-18 | 2016-03-15 | ピルビン酸デヒドロゲナーゼ変異体、それを含む微生物及びそれを用いるｌ−アミノ酸生産方法 |
| EP16765246.0A EP3272860B1 (en) | 2015-03-18 | 2016-03-15 | Pyruvate dehydrogenase mutant, microorganism comprising mutant, and method for producing l-amino acid by using microorganism |
| RU2017134331A RU2683208C1 (ru) | 2015-03-18 | 2016-03-15 | Мутант пируватдегидрогеназы, микроорганизм, содержащий мутант, и способ получения L-аминокислоты с использованием микроорганизма |
| CN201680002649.4A CN106715687B (zh) | 2015-03-18 | 2016-03-15 | 丙酮酸脱氢酶突变体、包含突变体的微生物和通过使用微生物生产l-氨基酸的方法 |
| US15/557,013 US10526586B2 (en) | 2015-03-18 | 2016-03-15 | Pyruvate dehydrogenase variants, a microorganism comprising the same and a method for producing L-amino acid using the same |
| US16/426,453 US20190292526A1 (en) | 2015-03-18 | 2019-05-30 | Pyruvate dehydrogenase variants, a microorganism comprising the same and a method for producing l-amino acid using the same |
| JP2019163451A JP2020000250A (ja) | 2015-03-18 | 2019-09-06 | ピルビン酸デヒドロゲナーゼ変異体、それを含む微生物及びそれを用いるｌ−アミノ酸生産方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020150037654A KR101776375B1 (ko) | 2015-03-18 | 2015-03-18 | 피루브산 디하이드로게나아제 변이체, 이를 포함하는 미생물 및 이를 이용한 l-아미노산 생산 방법 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| KR20160113377A KR20160113377A (ko) | 2016-09-29 |
| KR101776375B1 KR101776375B1 (ko) | 2017-09-08 |
| KR101776375B9 true KR101776375B9 (ko) | 2022-01-11 |
Family
ID=56919058
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020150037654A KR101776375B1 (ko) | 2015-03-18 | 2015-03-18 | 피루브산 디하이드로게나아제 변이체, 이를 포함하는 미생물 및 이를 이용한 l-아미노산 생산 방법 |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US10526586B2 (ko) |
| EP (1) | EP3272860B1 (ko) |
| JP (2) | JP6625133B2 (ko) |
| KR (1) | KR101776375B1 (ko) |
| CN (1) | CN106715687B (ko) |
| BR (1) | BR112017019057B1 (ko) |
| DK (1) | DK3272860T5 (ko) |
| ES (1) | ES2959213T3 (ko) |
| HU (1) | HUE064139T2 (ko) |
| MY (1) | MY187636A (ko) |
| PL (1) | PL3272860T3 (ko) |
| RU (1) | RU2683208C1 (ko) |
| WO (1) | WO2016148490A1 (ko) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101947945B1 (ko) * | 2018-01-25 | 2019-02-13 | 씨제이제일제당 (주) | L-아미노산을 생산하는 코리네박테리움 속 미생물 및 이를 이용한 l-아미노산의 생산방법 |
| KR101947959B1 (ko) * | 2018-05-28 | 2019-02-13 | 씨제이제일제당 (주) | 변이형 호모세린 디하이드로게나제 및 이를 이용한 호모세린 또는 호모세린 유래 l-아미노산의 생산 방법 |
| KR102311391B1 (ko) | 2020-05-21 | 2021-10-12 | 씨제이제일제당 주식회사 | L- 분지쇄 아미노산 생산능이 강화된 미생물 및 이를 이용하여 l-분지쇄 아미노산을 생산하는 방법 |
| KR102344689B1 (ko) * | 2020-09-01 | 2021-12-29 | 씨제이제일제당 주식회사 | L-발린 생산 미생물 및 이를 이용한 l-발린 생산 방법 |
| US20220372451A1 (en) * | 2021-04-28 | 2022-11-24 | University Of Georgia Research Foundation, Inc. | Production of pyruvate or products derived from pyruvate using modified escherichia coli |
| CN113249347B (zh) * | 2021-07-14 | 2021-11-12 | 中国科学院天津工业生物技术研究所 | 丙酮酸脱氢酶的突变体及其用于生产l-氨基酸的方法 |
| CN113430151B (zh) * | 2021-08-13 | 2022-06-07 | 江苏澳创生物科技有限公司 | 一株谷氨酸棒杆菌及其在发酵生产l-缬氨酸中的应用 |
| CN113683667B (zh) * | 2021-08-25 | 2024-06-25 | 宁夏伊品生物科技股份有限公司 | Yh66-rs10865基因改造得到的工程菌及其在制备缬氨酸中的应用 |
| KR102703218B1 (ko) * | 2022-01-11 | 2024-09-06 | 대상 주식회사 | L-히스티딘 생산능이 향상된 에스케리치아 속 변이주 및 이를 이용한 l-히스티딘의 생산 방법 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR940001307B1 (ko) | 1991-12-27 | 1994-02-19 | 제일제당 주식회사 | L-라이신을 생산하는 신규 미생물 |
| PE20001375A1 (es) * | 1998-12-18 | 2000-12-14 | Ajinomoto Kk | Metodo para producir acido l-glutamico por fermentacion |
| TW200734459A (en) * | 1999-10-04 | 2007-09-16 | Ajinomoto Kk | Genes for heat resistant enzymes of amino acid biosynthetic pathway derived from thermophilic coryneform bacteria |
| DE102005045301A1 (de) * | 2005-09-22 | 2007-04-05 | Degussa Ag | Verfahren zur Herstellung von organisch-chemischen Verbindungen unter Verwendung coryneformer Bakterien |
| DE102006032634A1 (de) | 2006-07-13 | 2008-01-17 | Evonik Degussa Gmbh | Verfahren zur Herstellung von L-Aminosäuren |
| EP2066782A4 (en) | 2006-09-15 | 2010-02-03 | Cj Cheiljedang Corp | CORYNEBACTERIA WITH IMPROVED L-LYSINE PRODUCTIVITY AND METHOD FOR THE PREPARATION OF L-LYSINE THEREWITH |
| RU2496867C2 (ru) * | 2011-04-25 | 2013-10-27 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО "АГРИ") | Способ получения l-аминокислоты семейства глутамата с использованием коринеформной бактерии |
| ES2699747T3 (es) * | 2011-04-29 | 2019-02-12 | Danisco Us Inc | Microorganismos recombinantes para la producción potenciada de mevalonato, isopreno e isoprenoides |
| KR101117022B1 (ko) | 2011-08-16 | 2012-03-16 | 씨제이제일제당 (주) | Ｌ-발린 생산능이 향상된 미생물 및 이를 이용한 ｌ-발린 제조방법 |
| KR101721722B1 (ko) | 2013-03-11 | 2017-04-10 | 씨제이제일제당 (주) | L-발린 생산능이 향상된 균주 및 이를 이용한 l-발린 생산방법 |
| CN104004678A (zh) * | 2014-05-05 | 2014-08-27 | 江南大学 | 一株高产l-缬氨酸的谷氨酸棒状杆菌工程菌的构建及其发酵产l-缬氨酸的方法 |
-
2015
- 2015-03-18 KR KR1020150037654A patent/KR101776375B1/ko active IP Right Review Request
-
2016
- 2016-03-15 JP JP2017548259A patent/JP6625133B2/ja active Active
- 2016-03-15 CN CN201680002649.4A patent/CN106715687B/zh active Active
- 2016-03-15 PL PL16765246.0T patent/PL3272860T3/pl unknown
- 2016-03-15 MY MYPI2017703025A patent/MY187636A/en unknown
- 2016-03-15 DK DK16765246.0T patent/DK3272860T5/da active
- 2016-03-15 RU RU2017134331A patent/RU2683208C1/ru active
- 2016-03-15 EP EP16765246.0A patent/EP3272860B1/en active Active
- 2016-03-15 HU HUE16765246A patent/HUE064139T2/hu unknown
- 2016-03-15 US US15/557,013 patent/US10526586B2/en active Active
- 2016-03-15 BR BR112017019057-5A patent/BR112017019057B1/pt active IP Right Grant
- 2016-03-15 WO PCT/KR2016/002594 patent/WO2016148490A1/ko active Application Filing
- 2016-03-15 ES ES16765246T patent/ES2959213T3/es active Active
-
2019
- 2019-05-30 US US16/426,453 patent/US20190292526A1/en not_active Abandoned
- 2019-09-06 JP JP2019163451A patent/JP2020000250A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| JP2018512132A (ja) | 2018-05-17 |
| KR101776375B1 (ko) | 2017-09-08 |
| HUE064139T2 (hu) | 2024-03-28 |
| JP6625133B2 (ja) | 2019-12-25 |
| RU2683208C1 (ru) | 2019-03-26 |
| ES2959213T3 (es) | 2024-02-21 |
| US10526586B2 (en) | 2020-01-07 |
| US20190292526A1 (en) | 2019-09-26 |
| EP3272860A4 (en) | 2018-08-08 |
| JP2020000250A (ja) | 2020-01-09 |
| KR20160113377A (ko) | 2016-09-29 |
| BR112017019057B1 (pt) | 2024-03-05 |
| BR112017019057A2 (pt) | 2018-04-17 |
| US20180057798A1 (en) | 2018-03-01 |
| CN106715687A (zh) | 2017-05-24 |
| PL3272860T3 (pl) | 2024-02-05 |
| EP3272860B1 (en) | 2023-08-09 |
| CN106715687B (zh) | 2021-04-27 |
| MY187636A (en) | 2021-10-05 |
| WO2016148490A1 (ko) | 2016-09-22 |
| DK3272860T5 (da) | 2024-06-10 |
| DK3272860T3 (da) | 2023-10-30 |
| EP3272860A1 (en) | 2018-01-24 |
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| AMND | Amendment | ||
| X701 | Decision to grant (after re-examination) | ||
| GRNT | Written decision to grant | ||
| J204 | Request for invalidation trial [patent] | ||
| J301 | Trial decision |
Free format text: TRIAL NUMBER: 2021100000223; TRIAL DECISION FOR INVALIDATION REQUESTED 20210125 Effective date: 20211019 |
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| J121 | Written withdrawal of request for trial |