DK2295558T3 - Xylanasevarianter - Google Patents

Xylanasevarianter Download PDF

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Publication number
DK2295558T3
DK2295558T3 DK10178666.3T DK10178666T DK2295558T3 DK 2295558 T3 DK2295558 T3 DK 2295558T3 DK 10178666 T DK10178666 T DK 10178666T DK 2295558 T3 DK2295558 T3 DK 2295558T3
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Denmark
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DK10178666.3T
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Inventor
Ole Sibbesen
Jens Frisbaek Soerensen
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Dupont Nutrition Biosci Aps
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Priority claimed from GB0005585A external-priority patent/GB0005585D0/en
Priority claimed from GB0015751A external-priority patent/GB0015751D0/en
Application filed by Dupont Nutrition Biosci Aps filed Critical Dupont Nutrition Biosci Aps
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Publication of DK2295558T3 publication Critical patent/DK2295558T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Detergent Compositions (AREA)
  • Paper (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)

Claims (11)

  1. XYLAN ASE V ARI ANTER
    1. Fremgangsmåde til ændring af sensitiviteten for et Familie-ll-xylanasepolypeptid over for en hæmmer, hvilken fremgangsmåde omfatter: (a) modificering af én eller flere af polypeptidets aminosyrerester; hvor modifikationerne er ved en hvilken som helst af positionerne 11, 12, 13, 15, 17, 29, 31, 32, 34, 113, 114, 119, 120, 121, 122, 123, 124 eller 175 af B. sntiif/fs-aminosyresekvensen ifølge SEQ. ID. NO. 1. eller ved én eller flere ækvivalente positioner i andre homologe xylanaser, hvor mindst én af aminosyremodifikationerne er mindst ved position 11 af B. sntiif/fs-aminosyresekvensen ifølge SEQ. ID. NO. 1 eller ved én eller flere ækvivalente positioner i andre homologe xylanaser; (b) måling af xylanasepolypeptidets sensitivitet over for en xylanasehæmmer og (c) måling af xylanasepolypeptidets effekt på en melopslæmnings viskositet; hvor peptidet eller fragmentet deraf har reduceret sensitivitet over for en xylanasehæmmer sammenlignet med moderxylanaseenzymet; hvor peptidet eller fragmentet deraf endvidere sænker en melopslæmnings viskositet mere end moderxylanaseenzymet.
  2. 2. Fremgangsmåde til ændring af sensitiviteten for et Familie-ll-xylanasepolypeptid over for en hæmmer, hvilken fremgangsmåde omfatter: (a) modificering af to eller flere polypeptidets aminosyrerester; hvor modifikationerne er af to eller flere af positionerne 11, 12, 13, 15, 17, 29, 31, 32, 34, 113, 114, 119, 120, 121, 122, 123, 124 og 175 af B. subtilis-aminosyresekvensen vist som SEQ. ID. NO. 1, eller ved én eller flere ækvivalente positioner i andre homologe xylanaser; hvor én af aminosyremodifikationerne er ved position 11; (b) måling af xylanasepolypeptidets sensitivitet over for en xylanasehæmmer og (c) måling af xylanasepolypeptidets effekt på en melopslæmnings viskositet; hvor polypeptidet eller fragmentet deraf har reduceret sensitivitet over for en xylanasehæmmer sammenlignet med moderxylanaseenzymet; endvidere hvor peptidet eller fragmentet deraf sænker en melopslæmnings viskositet mere end moderxylanaseenzymet.
  3. 3. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor aminosyremodifikationen sker ved målrettet mutagenese.
  4. 4. Fremgangsmåde ifølge et hvilket som helst foregående krav, hvor én af aminosyremodifikationerne er en hvilken som helst af: D11Y, DUN, D11F, D11K, DIIS, D11W, G12F, G13F, I15K, N17K, N17Y, N17D, N29K, N29Y, N29D, S31K, S31Y, S31D, N32K, G34D, G34F, G34T, Y113A, Y113D, Y113K, N114A, N114D, N114F, N114K, D119K, D119Y, D119N, G120K, G120D, G120F, G120Y, G120N, D121N, D121K, D121F, D121A, R122D, R122F, R122A, T123K, T123Y, T123D, T124K, T124Y, T124D, Q175E, Q175S og Q175L.
  5. 5. Fremgangsmåde ifølge et hvilket som helst foregående krav, hvor én af aminosyremodifikationerne er en hvilken som helst af: D11Y, DUN, D11F, D11K, DIIS eller D11W.
  6. 6. Fremgangsmåde ifølge et hvilket som helst foregående krav, hvor én af aminosyremodifikationeme erDllF.
  7. 7. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor to af aminosyremodifikationeme er D11F og R122D.
  8. 8. Anvendelse af en xylanasepolypeptidvariant tilvejebragt ved hjælp af fremgangsmåden ifølge et hvilket som helst af kravene 1 til 7 i en fremgangsmåde til modificering af plantematerialer.
  9. 9. Anvendelse af en xylanasepolypeptidvariant tilvejebragt ved hjælp af fremgangsmåden ifølge et hvilket som helst af kravene 1 til 7 i én eller flere af: bageri, dyrefoder, stivelsesproduktion, melseparation (vådformaling) og papir- og pulpproduktion.
  10. 10. Anvendelse af en xylanasepolypeptidvariant tilvejebragt ved hjælp af fremgangsmåden ifølge et hvilket som helst af kravene 1 til 7 i én eller flere af: forarbejdning af korn, stivelsesproduktion, dyrefoder, i forarbejdning af træ, forbedring af processen til blegning af træpulp.
  11. 11. Anvendelse af en xylanasepolypeptidvariant tilvejebragt ved hjælp af fremgangsmåden ifølge et hvilket som helst af kravene 1 til 7 til at ændre viskositeten, der er afledt af tilstedeværelsen af hemicellulose eller arabinoxylan i en opløsning eller et system, der omfatter plantecellevægmateriale.
DK10178666.3T 2000-03-08 2001-03-08 Xylanasevarianter DK2295558T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0005585A GB0005585D0 (en) 2000-03-08 2000-03-08 Enzyme
GB0015751A GB0015751D0 (en) 2000-06-27 2000-06-27 Enzyme
EP01912068A EP1263941B1 (en) 2000-03-08 2001-03-08 Xylanase variants having altered sensitivity to xylanase inhibitors

Publications (1)

Publication Number Publication Date
DK2295558T3 true DK2295558T3 (da) 2018-01-22

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DK10178666.3T DK2295558T3 (da) 2000-03-08 2001-03-08 Xylanasevarianter
DK01912068.2T DK1263941T3 (da) 2000-03-08 2001-03-08 Xylanasevarianter med ændret sensitivitet overfor xylanaseinhibitorer

Family Applications After (1)

Application Number Title Priority Date Filing Date
DK01912068.2T DK1263941T3 (da) 2000-03-08 2001-03-08 Xylanasevarianter med ændret sensitivitet overfor xylanaseinhibitorer

Country Status (15)

Country Link
US (3) US7611881B2 (da)
EP (3) EP2295558B1 (da)
JP (1) JP4795604B2 (da)
AT (1) ATE491024T1 (da)
AU (1) AU785107C (da)
BR (1) BR0108750A (da)
CA (1) CA2399700C (da)
DE (1) DE60143606D1 (da)
DK (2) DK2295558T3 (da)
ES (1) ES2656440T3 (da)
MX (1) MXPA02008817A (da)
NZ (1) NZ520659A (da)
PL (1) PL212113B1 (da)
PT (1) PT1263941E (da)
WO (1) WO2001066711A1 (da)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6936289B2 (en) 1995-06-07 2005-08-30 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
US6764699B2 (en) * 2000-12-05 2004-07-20 Roberto Gonzalez Barrera Corn tortillas with improved texture retention using an enzyme blend in nixtamalized corn flour
DK1387616T3 (da) 2001-05-18 2007-09-24 Danisco Fremgangsmåde til fremstilling af en dej med et enzym
GB0121387D0 (en) * 2001-09-04 2001-10-24 Danisco Modified hydrolases
US7510860B1 (en) * 2001-11-21 2009-03-31 National Research Council Of Canada Xylanases with enhanced thermophilicity and alkalophilicity
CN1675365A (zh) 2002-06-14 2005-09-28 戴弗萨公司 木聚糖酶、编码木聚糖酶的核酸以及制备和应用它们的方法
EP1542538A2 (en) 2002-09-27 2005-06-22 Danisco A/S Lipase inhibitors and uses thereof
EP1668125B1 (en) * 2003-09-15 2014-06-18 Danisco US Inc. Modified enzymes, methods to produce modified enzymes and uses thereof
JP5604032B2 (ja) 2004-07-16 2014-10-08 デュポン ニュートリション バイオサイエンシーズ エーピーエス 食用油の酵素的脱ガム方法
DK1926753T3 (da) 2004-12-22 2011-03-14 Novozymes As Hybridenzymer, der består af en endoamylase som første aminosyresekvens og et kulhydratbindingsmodul som anden aminosyresekvens
EP1752533A1 (en) * 2005-08-12 2007-02-14 Institut National de la Recherche Agronomique Fusion proteins between plant cell-wall degrading enzymes, and their uses
USRE45660E1 (en) 2006-02-14 2015-09-01 Bp Corporation North America Inc. Xylanases, nucleic acids encoding them and methods for making and using them
WO2007115391A1 (en) * 2006-04-12 2007-10-18 National Research Council Of Cananda Modification of xylanases to increase thermophilicity, thermostability and alkalophilicity
CA2655478A1 (en) * 2006-06-16 2007-12-21 Syngenta Participations Ag Catalytically inactive proteins and method for recovery of enzymes from plant-derived materials
CN101522045A (zh) * 2006-09-29 2009-09-02 诺维信公司 用于动物饲料的木聚糖酶
NZ584967A (en) 2007-10-03 2012-08-31 Verenium Corp Xylanases, nucleic acids encoding them and methods for making and using them
AU2009329608B2 (en) 2008-12-23 2013-12-05 Dupont Nutrition Biosciences Aps Polypeptides with xylanase activity
WO2010072226A1 (en) * 2008-12-23 2010-07-01 Danisco A/S Polypeptides with xylanase activity
US8951751B2 (en) * 2008-12-23 2015-02-10 Dupont Nutrition Biosciences Aps Polypeptides with xylanase activity
US20130071884A1 (en) 2009-11-06 2013-03-21 Agrivida, Inc. Plants expressing cell wall degrading enzymes and expression vectors
CA2791353A1 (en) * 2010-03-03 2011-09-09 Novozymes, Inc. Xylanase variants and polynucleotides encoding same
CN106244622B (zh) 2011-03-07 2020-06-12 谷万达公司 在工程植物中表达多种蛋白的表达盒和表达载体及制备工程植物和生产可溶性糖的方法
WO2016073610A1 (en) * 2014-11-07 2016-05-12 Novozymes A/S Xylanase based bleach boosting
CN104774819A (zh) 2015-02-24 2015-07-15 诺维信公司 木聚糖酶颗粒
EP3277828A1 (en) 2015-03-04 2018-02-07 DuPont Nutrition Biosciences ApS Cereal grain processing
KR20180117166A (ko) 2016-03-04 2018-10-26 다니스코 유에스 인크. 미생물에서의 단백질 생산을 위한 유전자 조작 리보솜 프로모터
EP3703661A1 (en) 2017-11-02 2020-09-09 Danisco US Inc. Freezing point depressed solid matrix compositions for melt granulation of enzymes
US20210076704A1 (en) 2017-12-20 2021-03-18 Dsm Ip Assets B.V. Animal feed compositions and uses thereof
BR112021004833A2 (pt) 2018-09-17 2021-06-08 Dsm Ip Assets B.V. composições de ração animal e usos das mesmas
CN110079512A (zh) * 2019-03-19 2019-08-02 天津科技大学 一种高温耐盐耐酸碱木聚糖酶Xyn22、基因、重组载体和菌株、制备方法和应用
CA3234382A1 (en) 2021-11-09 2023-05-19 Tina Ploss Improved xylanases
CN114350642B (zh) * 2022-03-18 2022-05-31 中国农业科学院北京畜牧兽医研究所 一种提高木聚糖酶的木二糖产量的方法及突变体和应用
CN116396953B (zh) * 2022-11-22 2023-12-19 天典(广东)生物科技有限公司 木聚糖酶突变体及其应用和重组枯草芽孢杆菌

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US575984A (en) * 1897-01-26 Robert pierpont
US5200215A (en) 1988-04-20 1993-04-06 Nabisco, Inc. Enzyme treated low moisture content comestible products
US5550318A (en) 1990-04-17 1996-08-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
NL9001388A (nl) 1990-06-19 1992-01-16 Unilever Nv Recombinant dna, cel die daarvan afgeleid dna bevat, enzym waarvoor het recombinant dna codeert en toepassingen daarvan.
WO1993025693A1 (en) 1992-06-17 1993-12-23 The Agricultural And Food Research Council Recombinant xylanases
DE4226528A1 (de) * 1992-08-11 1994-02-17 Roehm Gmbh Batterien-Xylanase, Verfahren zu ihrer Herstellung sowie dafür geeigneter Bakterienstamm, Plasmid mit zugehörigem Strukturgen, sowie Backmittel und Backverfahren zur Herstellung von Brot und Backwaren unter Verwendung der Xylanase
EP0746206B1 (en) 1994-03-02 2004-09-08 Novozymes A/S Processing plant material with xylanase
JP3435946B2 (ja) 1994-12-21 2003-08-11 王子製紙株式会社 耐熱性キシラナーゼ
US5759840A (en) * 1996-09-09 1998-06-02 National Research Council Of Canada Modification of xylanase to improve thermophilicity, alkalophilicity and thermostability
JP5044067B2 (ja) 1997-04-30 2012-10-10 ケー．ユー． ルヴァン リサーチ アンド ディヴェロプメント キシラン分解酵素の阻害剤
US6253168B1 (en) * 1998-05-12 2001-06-26 Isis Pharmaceuticals, Inc. Generation of virtual combinatorial libraries of compounds
EP0979830A1 (en) * 1998-08-12 2000-02-16 Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Tno A novel class of xylanase inhibitors
EP1141254B1 (en) * 1998-12-23 2006-03-08 Danisco A/S Use of a xylanase for preparing a foodstuff
JP2002543791A (ja) 1999-05-12 2002-12-24 ゼンコー 新規な熱安定性の親アルカリ性キシラナーゼ
US6682923B1 (en) * 1999-05-12 2004-01-27 Xencor Thermostable alkaliphilic xylanase

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Publication number Publication date
US7611881B2 (en) 2009-11-03
WO2001066711A1 (en) 2001-09-13
US20030180895A1 (en) 2003-09-25
NZ520659A (en) 2005-06-24
US20090155413A1 (en) 2009-06-18
BR0108750A (pt) 2002-12-24
EP1263941B1 (en) 2010-12-08
DK1263941T3 (da) 2011-03-14
AU4098401A (en) 2001-09-17
JP4795604B2 (ja) 2011-10-19
ATE491024T1 (de) 2010-12-15
EP1263941A1 (en) 2002-12-11
CA2399700C (en) 2013-10-01
DE60143606D1 (de) 2011-01-20
PT1263941E (pt) 2011-01-20
PL212113B1 (pl) 2012-08-31
US7527957B2 (en) 2009-05-05
US20050271769A1 (en) 2005-12-08
ES2656440T3 (es) 2018-02-27
AU785107B2 (en) 2006-09-14
AU785107C (en) 2007-05-03
JP2003525629A (ja) 2003-09-02
EP2295558A1 (en) 2011-03-16
EP3339434A1 (en) 2018-06-27
PL362558A1 (en) 2004-11-02
CA2399700A1 (en) 2001-09-13
MXPA02008817A (es) 2003-02-12
WO2001066711A8 (en) 2001-11-01
EP2295558B1 (en) 2017-11-01

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