DK2382310T3 - Polypeptider med xylanaseaktivitet - Google Patents
Polypeptider med xylanaseaktivitet Download PDFInfo
- Publication number
- DK2382310T3 DK2382310T3 DK09834136.5T DK09834136T DK2382310T3 DK 2382310 T3 DK2382310 T3 DK 2382310T3 DK 09834136 T DK09834136 T DK 09834136T DK 2382310 T3 DK2382310 T3 DK 2382310T3
- Authority
- DK
- Denmark
- Prior art keywords
- polypeptide
- amino acid
- xylanase
- seq
- acid sequence
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2477—Hemicellulases not provided in a preceding group
- C12N9/248—Xylanases
- C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Fodder In General (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Bakery Products And Manufacturing Methods Therefor (AREA)
Claims (10)
- POLYPEPTIDER MED XYLANASEAKTIVITET1. Polypeptid, der har xylanaseaktivitet og omfatter en aminosyresekvens, hvilken aminosyresekvens er mindst 75 % identisk med aminosyresekvensen ifølge SEQ ID NO: 1, og hvilket polypeptid har i) én eller to aminosyremodifikationer i en position, der er udvalgt fra: 12 og 13; og ii) én eller flere yderligere aminosyresubstitutioner i en position, der er udvalgt fra: 15, 34, 54, 77, 81, 82, 99, 104,110, 113, 114,118, 122, 141, 154, 159, 162,164, 166, 175 og 179, hvor positionerne er bestemt som positionen, der svarer til positionen af B. subtilis-xylanasesekvensen ifølge SEQ ID NO: 1 ved alignment; hvor aminosyremodifikationen i position 12 og 13 er en aminosyresubstitution med tyrosin.
- 2. Polypeptid ifølge krav 1, der omfatter én eller flere aminosyresubstitutioner, der er udvalgt fra gruppen bestående af: 13Y, 15Y, 34K, 77V, 77M, 77Y, 77L, 77S, 811, 821, 99Y, 104W, 110A, 113D, 113A, 114F, 114D, 114Y, 118V, 122F, 122D, 154R, 159D, 162E, 162D, 164F, 166F, 175L, 175K, 175E, 175Y og 179Y, hvilken eller hvilke positioner bestemmes som den tilsvarende position af B. subtilis -aminosyresekvensen ifølge SEQ ID NO: 1.
- 3. Polypeptid ifølge et hvilket som helst af kravene 1-2, hvor polypeptidet er mindst 80, 85, 90, 95 eller 98 % identisk med den sekvens, med hvilken den har den højeste procentvise identitet, der er udvalgt fra SEQ ID NO: 1.
- 4. Polypeptid ifølge et hvilket som helst af kravene 1-3, der har en β-jelly roll-fold.
- 5. Polypeptid ifølge et hvilket som helst af kravene 1-4, hvilket polypeptid har et samlet antal aminosyrer på mindre end 250, såsom mindre end 240, såsom mindre end 230, såsom mindre end 220, såsom mindre end 210, såsom mindre end 200 aminosyrer, såsom i intervallet på mellem 160 til 240, såsom i intervallet på mellem 160 til 220 aminosyrer.
- 6. Polypeptid ifølge et hvilket som helst af kravene 1-5, hvilket polypeptid omfatter én eller flere aminosyresubstitutioner, der er udvalgt fra gruppen bestående af: 13Y, 110A, 122D, 113A, 13Y, 113D, 175L, 122F, 34K, 99Y, 104W, 154R, 159D, 175K, 811, 166F, 162E, 162D, 164F, 114D, 114Y, 114F, 118V, 175K, 77L, 77M, 77S, 77V og 77Y, hvilken eller hvilke positioner bestemmes som den tilsvarende af B. -aminosyresekvensen ifølge SEQ ID NO: 1.
- 7. Fremgangsmåde til fremstilling af et polypeptid ifølge et hvilket som helst af kravene 1-6, hvilken fremgangsmåde omfatter ekspression af en nukleotidsekvens, der koder for polypeptidet, og eventuelt isolering af og/eller oprensning af polypeptidet efter ekspression.
- 8. Nukleotidsekvens, der koder for et polypeptid ifølge et hvilket som helst af kravene 1-6.
- 9. Sammensætning, der omfatter polypeptidet ifølge et hvilket som helst af kravene 1-6, eller polypeptid fremstillet ifølge krav 7 eller nukleotidsekvens ifølge krav 8 sammenblandet med en ikke-toksisk bestanddel.
- 10. Anvendelse af polypeptidet ifølge et hvilket som helst af kravene 1-6 eller et polypeptid, der er fremstillet ifølge krav 7, eller nukleotidsekvensen ifølge krav 8 sammenblandet med en ikke-toksisk bestanddel eller sammensætning ifølge krav 9 i en fremgangsmåde til modificering af plantematerialer.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP08172755 | 2008-12-23 | ||
| US14617009P | 2009-01-21 | 2009-01-21 | |
| PCT/DK2009/050352 WO2010072225A1 (en) | 2008-12-23 | 2009-12-23 | Polypeptides with xylanase activity |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2382310T3 true DK2382310T3 (da) | 2016-12-12 |
Family
ID=40494933
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK09834136.5T DK2382310T3 (da) | 2008-12-23 | 2009-12-23 | Polypeptider med xylanaseaktivitet |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US8765438B2 (da) |
| EP (1) | EP2382310B1 (da) |
| JP (2) | JP2012513207A (da) |
| KR (1) | KR20110117663A (da) |
| CN (1) | CN102300985A (da) |
| AU (1) | AU2009329608B2 (da) |
| CA (1) | CA2747224C (da) |
| DK (1) | DK2382310T3 (da) |
| EA (1) | EA201170879A1 (da) |
| ES (1) | ES2602792T3 (da) |
| MX (2) | MX347545B (da) |
| UA (1) | UA103215C2 (da) |
| WO (1) | WO2010072225A1 (da) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102292436A (zh) | 2008-12-23 | 2011-12-21 | 丹尼斯科公司 | 具有木聚糖酶活性的多肽 |
| AU2012307299C1 (en) | 2011-09-14 | 2018-10-25 | International N&H Denmark Aps | Compositions comprising enzymes with endo - 1, 4 - beta - xylanase activity and enzymes with endo - 1, 3 (4) - beta glucanase activity |
| MX2015001203A (es) | 2012-08-03 | 2015-04-14 | Dupont Nutrition Biosci Aps | Enzimas. |
| WO2014061763A1 (ja) * | 2012-10-19 | 2014-04-24 | 独立行政法人産業技術総合研究所 | 新規キシラナーゼ |
| WO2014140167A1 (en) | 2013-03-14 | 2014-09-18 | Dsm Ip Assets B.V. | Cell wall deconstruction enzymes of malbranchea cinnamomea and uses thereof |
| WO2014140165A1 (en) | 2013-03-14 | 2014-09-18 | Dsm Ip Assets B.V. | Cell wall deconstruction enzymes of paecilomyces byssochlamydoides and uses thereof |
| US9850512B2 (en) | 2013-03-15 | 2017-12-26 | The Research Foundation For The State University Of New York | Hydrolysis of cellulosic fines in primary clarified sludge of paper mills and the addition of a surfactant to increase the yield |
| GB201401699D0 (en) * | 2014-01-31 | 2014-03-19 | Dupont Nutrition Biosci Aps | Protein |
| US9951363B2 (en) | 2014-03-14 | 2018-04-24 | The Research Foundation for the State University of New York College of Environmental Science and Forestry | Enzymatic hydrolysis of old corrugated cardboard (OCC) fines from recycled linerboard mill waste rejects |
| EP3017706A1 (en) | 2014-11-05 | 2016-05-11 | Dupont Nutrition Biosciences ApS | Enzymes for malting |
| CN107404915A (zh) | 2015-03-04 | 2017-11-28 | 杜邦营养生物科学有限公司 | 谷粒加工 |
| CN109071615A (zh) | 2016-03-04 | 2018-12-21 | 丹尼斯科美国公司 | 用于在微生物中产生蛋白质的工程化核糖体启动子 |
| WO2019089898A1 (en) | 2017-11-02 | 2019-05-09 | Danisco Us Inc | Freezing point depressed solid matrix compositions for melt granulation of enzymes |
| CN109971737B (zh) * | 2017-12-28 | 2020-11-06 | 中粮集团有限公司 | 木聚糖酶HoXyn11A的突变体及其制备方法和用途 |
| WO2023225459A2 (en) | 2022-05-14 | 2023-11-23 | Novozymes A/S | Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections |
| KR102700160B1 (ko) * | 2021-10-29 | 2024-08-28 | 씨제이제일제당 주식회사 | 자일라나제 활성을 갖는 변이형 폴리펩티드 |
| WO2023083806A1 (en) | 2021-11-09 | 2023-05-19 | Ab Enzymes Gmbh | Improved xylanases |
| KR20230167903A (ko) * | 2022-06-03 | 2023-12-12 | 씨제이제일제당 (주) | 자일라나제 활성을 갖는 변이형 폴리펩티드 |
| WO2024163288A1 (en) | 2023-02-01 | 2024-08-08 | International N&H Denmark Aps | Novel methods and compositions for improving mash filterabililty and yield in brewing |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4226528A1 (de) | 1992-08-11 | 1994-02-17 | Roehm Gmbh | Batterien-Xylanase, Verfahren zu ihrer Herstellung sowie dafür geeigneter Bakterienstamm, Plasmid mit zugehörigem Strukturgen, sowie Backmittel und Backverfahren zur Herstellung von Brot und Backwaren unter Verwendung der Xylanase |
| ATE210180T1 (de) | 1995-01-26 | 2001-12-15 | Novozymes As | Xylanase beinhaltende futterzusätze für tiere |
| US6682923B1 (en) * | 1999-05-12 | 2004-01-27 | Xencor | Thermostable alkaliphilic xylanase |
| NZ520659A (en) * | 2000-03-08 | 2005-06-24 | Danisco | Xylanase variants having altered sensitivity to xylanase inhibitors caused by mutations in the lid region |
| EP1184460A1 (en) * | 2000-08-29 | 2002-03-06 | Dsm N.V. | Modified fungal xylanases |
| GB0121387D0 (en) * | 2001-09-04 | 2001-10-24 | Danisco | Modified hydrolases |
| NZ537597A (en) * | 2002-06-14 | 2008-07-31 | Diversa Corp | Xylanases, nucleic acids encoding them and methods for making and using them |
| CN101415823B (zh) | 2003-09-15 | 2012-10-17 | 金克克国际有限公司 | 修饰酶、生产修饰酶的方法及其用途 |
| MXPA06006670A (es) * | 2003-12-19 | 2007-03-21 | Syngenta Participations Ag | Xilanasas expresadas microbialmente y su uso como aditivos de forraje y otros usos. |
| JP2007527726A (ja) * | 2004-03-08 | 2007-10-04 | シンジェンタ パーティシペーションズ アクチェンゲゼルシャフト | 自己プロセシング植物および植物部分 |
| EP1727903B1 (en) * | 2004-03-25 | 2011-10-05 | Iogen Bio-Products Corporation | Modified xylanases exhibiting improved expression |
| CA2638801C (en) | 2006-02-14 | 2016-12-13 | Verenium Corporation | Xylanases, nucleic acids encoding them and methods for making and using them |
| CN102292436A (zh) | 2008-12-23 | 2011-12-21 | 丹尼斯科公司 | 具有木聚糖酶活性的多肽 |
| CN102317451A (zh) | 2008-12-23 | 2012-01-11 | 丹尼斯科公司 | 具有木聚糖酶活性的多肽 |
-
2009
- 2009-12-23 MX MX2015005025A patent/MX347545B/es unknown
- 2009-12-23 JP JP2011542679A patent/JP2012513207A/ja active Pending
- 2009-12-23 MX MX2011006640A patent/MX2011006640A/es active IP Right Grant
- 2009-12-23 UA UAA201109171A patent/UA103215C2/uk unknown
- 2009-12-23 AU AU2009329608A patent/AU2009329608B2/en not_active Ceased
- 2009-12-23 CN CN2009801556534A patent/CN102300985A/zh active Pending
- 2009-12-23 ES ES09834136.5T patent/ES2602792T3/es active Active
- 2009-12-23 KR KR1020117017490A patent/KR20110117663A/ko not_active Application Discontinuation
- 2009-12-23 DK DK09834136.5T patent/DK2382310T3/da active
- 2009-12-23 US US13/141,468 patent/US8765438B2/en active Active
- 2009-12-23 EA EA201170879A patent/EA201170879A1/ru unknown
- 2009-12-23 WO PCT/DK2009/050352 patent/WO2010072225A1/en active Application Filing
- 2009-12-23 CA CA2747224A patent/CA2747224C/en active Active
- 2009-12-23 EP EP09834136.5A patent/EP2382310B1/en active Active
-
2014
- 2014-05-09 US US14/274,268 patent/US9297002B2/en active Active
-
2015
- 2015-03-11 JP JP2015048334A patent/JP2015163070A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US9297002B2 (en) | 2016-03-29 |
| AU2009329608A1 (en) | 2011-07-07 |
| US20120028306A1 (en) | 2012-02-02 |
| ES2602792T3 (es) | 2017-02-22 |
| EA201170879A1 (ru) | 2012-02-28 |
| WO2010072225A1 (en) | 2010-07-01 |
| KR20110117663A (ko) | 2011-10-27 |
| CN102300985A (zh) | 2011-12-28 |
| CA2747224C (en) | 2019-09-03 |
| US8765438B2 (en) | 2014-07-01 |
| EP2382310B1 (en) | 2016-08-24 |
| JP2015163070A (ja) | 2015-09-10 |
| CA2747224A1 (en) | 2010-07-01 |
| EP2382310A1 (en) | 2011-11-02 |
| JP2012513207A (ja) | 2012-06-14 |
| US20150024466A1 (en) | 2015-01-22 |
| MX347545B (es) | 2017-05-02 |
| UA103215C2 (uk) | 2013-09-25 |
| EP2382310A4 (en) | 2012-08-22 |
| MX2011006640A (es) | 2011-07-29 |
| AU2009329608B2 (en) | 2013-12-05 |
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