DK204684A - Humant vaeksthormon frit for andre proteiner forbundet med dets native miljoe, udtrykkelsesmedium, som kan frembringe produktion af prae-hgh i en prokaryotisk vaertscelle, prokaryotisk vaert transformeret dermed og fremgangsmaade til fremstilling af modent hgh - Google Patents

Humant vaeksthormon frit for andre proteiner forbundet med dets native miljoe, udtrykkelsesmedium, som kan frembringe produktion af prae-hgh i en prokaryotisk vaertscelle, prokaryotisk vaert transformeret dermed og fremgangsmaade til fremstilling af modent hgh Download PDF

Info

Publication number
DK204684A
DK204684A DK204684A DK204684A DK204684A DK 204684 A DK204684 A DK 204684A DK 204684 A DK204684 A DK 204684A DK 204684 A DK204684 A DK 204684A DK 204684 A DK204684 A DK 204684A
Authority
DK
Denmark
Prior art keywords
thermalged
prokaryotically
prae
procaryotic
hgh
Prior art date
Application number
DK204684A
Other languages
Danish (da)
English (en)
Other versions
DK204684D0 (da
Inventor
Gregory Lawrence Gray
Herbert Louis Heyneker
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of DK204684D0 publication Critical patent/DK204684D0/da
Publication of DK204684A publication Critical patent/DK204684A/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/78—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S930/00—Peptide or protein sequence
    • Y10S930/01—Peptide or protein sequence
    • Y10S930/12—Growth hormone, growth factor other than t-cell or b-cell growth factor, and growth hormone releasing factor; related peptides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S930/00—Peptide or protein sequence
    • Y10S930/01—Peptide or protein sequence
    • Y10S930/30—Signal or leader sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
DK204684A 1983-04-25 1984-04-24 Humant vaeksthormon frit for andre proteiner forbundet med dets native miljoe, udtrykkelsesmedium, som kan frembringe produktion af prae-hgh i en prokaryotisk vaertscelle, prokaryotisk vaert transformeret dermed og fremgangsmaade til fremstilling af modent hgh DK204684A (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/488,232 US4755465A (en) 1983-04-25 1983-04-25 Secretion of correctly processed human growth hormone in E. coli and Pseudomonas

Publications (2)

Publication Number Publication Date
DK204684D0 DK204684D0 (da) 1984-04-24
DK204684A true DK204684A (da) 1984-10-26

Family

ID=23938879

Family Applications (1)

Application Number Title Priority Date Filing Date
DK204684A DK204684A (da) 1983-04-25 1984-04-24 Humant vaeksthormon frit for andre proteiner forbundet med dets native miljoe, udtrykkelsesmedium, som kan frembringe produktion af prae-hgh i en prokaryotisk vaertscelle, prokaryotisk vaert transformeret dermed og fremgangsmaade til fremstilling af modent hgh

Country Status (9)

Country Link
US (1) US4755465A (ja)
EP (1) EP0127305A1 (ja)
JP (2) JPS6045595A (ja)
CA (1) CA1267099A (ja)
DK (1) DK204684A (ja)
ES (1) ES531896A0 (ja)
IL (1) IL71622A (ja)
NZ (1) NZ207924A (ja)
ZA (1) ZA843062B (ja)

Families Citing this family (85)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5618697A (en) * 1982-12-10 1997-04-08 Novo Nordisk A/S Process for preparing a desired protein
DK55685A (da) * 1985-02-07 1986-08-08 Nordisk Gentofte Enzym eller enzymkompleks med proteolytisk aktivitet
US4859600A (en) * 1983-04-25 1989-08-22 Genentech, Inc. Recombinant procaryotic cell containing correctly processed human growth hormone
IL71991A (en) 1983-06-06 1994-05-30 Genentech Inc Preparation of human FGI and FGE in their processed form through recombinant AND tranology in prokaryotes
BG49718A3 (en) * 1983-07-15 1992-01-15 Bio Technology General Corp Method for preparing of polypeptid with superoxiddismutasne activitty
DE122004000009I1 (de) * 1984-08-16 2004-11-18 Savient Pharmaceuticals Inc Methode zur Herstellung von menschlichen Wachstumshormonen.
IL76360A0 (en) * 1984-09-26 1986-01-31 Takeda Chemical Industries Ltd Mutual separation of proteins
US6534285B1 (en) 1984-12-24 2003-03-18 Genentech, Inc. Molecularly cloned acquired immunodeficiency syndrome polypeptides and their methods of use
US5853978A (en) * 1985-12-04 1998-12-29 Genentech, Inc. Molecularly cloned acquired immunodeficiency syndrome polypeptides and methods of use
US4861868A (en) * 1985-02-22 1989-08-29 Monsanto Company Production of proteins in procaryotes
DK151585D0 (da) * 1985-04-03 1985-04-03 Nordisk Gentofte Dna-sekvens
US4786501A (en) * 1985-07-15 1988-11-22 International Minerals & Chemical Corp. Cylindrical implants for the controlled release of growth hormones
US4865974A (en) * 1985-09-20 1989-09-12 Cetus Corporation Bacterial methionine N-terminal peptidase
US4870017A (en) * 1985-09-20 1989-09-26 Cetus Corporation Bacterial methionine N-terminal peptidase
US5013662A (en) * 1985-09-20 1991-05-07 Cetus Corporation Bacterial methionine n-terminal peptidase
FR2597114B1 (fr) * 1986-04-10 1994-02-25 Sanofi Procede microbiologique pour l'obtention d'une proteine par culture d'une souche bacterienne mutante
FR2599753B1 (fr) * 1986-06-05 1989-12-08 Sanofi Sa Sequence d'adn composite et son application pour la production de l'hormone de croissance
IT1228925B (it) * 1987-08-07 1991-07-10 Eniricerche Spa Procedimento per la preparazione dell'ormone della crescita umano
US5202119A (en) * 1991-06-28 1993-04-13 Genentech, Inc. Method of stimulating immune response
US5744139A (en) * 1991-06-28 1998-04-28 University Of Tennessee Research Corporation Insulin-like growth factor I (IGF-1) induced improvement of depressed T4/T8 ratios
US5317012A (en) * 1991-10-04 1994-05-31 The University Of Tennessee Research Corporation Human growth hormone induced improvement in depressed T4/T8 ratio
WO1993008292A1 (en) * 1991-10-16 1993-04-29 Agracetus, Inc. Particle-mediated transformation of animal somatic cells
JPH07118577B2 (ja) * 1992-12-02 1995-12-18 株式会社リコー 電子装置用基板の検査方法
GB9526733D0 (en) 1995-12-30 1996-02-28 Delta Biotechnology Ltd Fusion proteins
MX9605082A (es) 1996-10-24 1998-04-30 Univ Autonoma De Nuevo Leon Levaduras metilotroficas modificadas geneticamente para la produccion y secrecion de hormona de crecimiento humano.
JP4212470B2 (ja) * 2001-06-26 2009-01-21 アムジェン フレモント インク． Ｏｐｇｌへの抗体
ES2295370T3 (es) * 2001-07-06 2008-04-16 Lg Life Sciences Ltd. Nueva aminopeptidasa derivada de bacillus licheniformis, gen que codifica la aminopeptidasa, vector de expresion que contiene el gen, transformante y metodo para su preparacion.
WO2003068948A1 (en) 2002-02-13 2003-08-21 Dow Global Technologies Inc. Over-expression of extremozyme genes in pseudomonads and closely related bacteria
DE60332358D1 (de) * 2002-09-09 2010-06-10 Hanall Pharmaceutical Co Ltd Protease-resistente modifizierte interferon alpha polypeptide
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
EP1625157A2 (en) * 2003-05-09 2006-02-15 Pharmexa A/S Immunogenic human tnf alpha analogues with reduced cytotoxicity and methods of their preparation
KR20060069825A (ko) * 2003-08-01 2006-06-22 제넨테크, 인크. 제한된 다양성을 갖는 항체 ｃｄｒ 폴리펩티드 서열
KR101237651B1 (ko) * 2003-11-19 2013-02-27 다우 글로벌 테크놀로지스 엘엘씨 개선된 단백질 발현 시스템
EP2314602A1 (en) 2003-11-21 2011-04-27 Pfenex, Inc. Improved expression systems with SEC-system secretion
CN101151272A (zh) * 2003-12-01 2008-03-26 陶氏环球技术公司 在假单胞菌中生产重组二十面体病毒样颗粒
AU2005206951B2 (en) 2004-01-16 2010-08-19 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
JP4889505B2 (ja) 2004-02-02 2012-03-07 アンブレツクス・インコーポレイテツド 被修飾されたヒト成長ホルモンポリペプチドおよびこれらの使用
JP2008503217A (ja) * 2004-06-18 2008-02-07 アンブレツクス・インコーポレイテツド 新規抗原結合ポリペプチド及びそれらの使用
ATE502957T1 (de) * 2004-06-23 2011-04-15 Usv Ltd Chimeres, von den placenta- und hypophysenisoformen abgeleitetes humanes wachstumshormon und verfahren zur gewinnung der chimere
CA2569381A1 (en) * 2004-07-21 2006-08-31 Ambrx, Inc. Biosynthetic polypeptides utilizing non-naturally encoded amino acids
WO2009100255A2 (en) 2008-02-08 2009-08-13 Ambrx, Inc. Modified leptin polypeptides and their uses
AU2005269527B2 (en) 2004-07-26 2011-12-01 Pfenex Inc. Process for improved protein expression by strain engineering
US7998930B2 (en) 2004-11-04 2011-08-16 Hanall Biopharma Co., Ltd. Modified growth hormones
JP2008525032A (ja) 2004-12-22 2008-07-17 アンブレツクス・インコーポレイテツド 組換えヒト成長ホルモンを発現及び精製するための方法
KR101224781B1 (ko) 2004-12-22 2013-01-21 암브룩스, 인코포레이티드 비천연적으로 코딩된 아미노산을 포함하는 인간 성장호르몬의 조제물
WO2006068802A2 (en) * 2004-12-22 2006-06-29 Ambrx, Inc. COMPOSITIONS OF AMINOACYL-tRNA SYNTHETASE AND USES THEREOF
KR20070090023A (ko) 2004-12-22 2007-09-04 암브룩스, 인코포레이티드 변형 인간 성장 호르몬
CA2609205A1 (en) * 2005-06-03 2006-12-14 Ambrx, Inc. Improved human interferon molecules and their uses
AU2005335491B2 (en) * 2005-08-18 2010-11-25 Ambrx, Inc. Compositions of tRNA and uses thereof
WO2007056441A2 (en) 2005-11-07 2007-05-18 Genentech, Inc. Binding polypeptides with diversified and consensus vh/vl hypervariable sequences
ATE500215T1 (de) * 2005-11-08 2011-03-15 Ambrx Inc Beschleuniger für die modifikation nichtnatürlicher aminosäuren und nichtnatürlicher aminosäurepolypeptide
ES2547554T3 (es) * 2005-11-16 2015-10-07 Ambrx, Inc. Métodos y composiciones que comprenden aminoácidos no naturales
EP1973951A2 (en) * 2005-12-02 2008-10-01 Genentech, Inc. Binding polypeptides with restricted diversity sequences
EP1957540B1 (en) * 2005-12-02 2012-06-13 Genentech, Inc. Binding polypeptides and uses thereof
KR101423898B1 (ko) * 2005-12-14 2014-07-28 암브룩스, 인코포레이티드 비-천연 아미노산 및 폴리펩티드를 함유하는 조성물, 비-천연 아미노산 및 폴리펩티드를 포함하는 방법, 및 비-천연 아미노산 및 폴리펩티드의 용도
EP2069396B1 (en) * 2006-09-08 2015-10-21 Ambrx, Inc. Modified human plasma polypeptide or fc scaffolds and their uses
PT2064333E (pt) * 2006-09-08 2014-06-09 Ambrx Inc Supressor híbrido arnt para células de vertebrados
CN101541955B (zh) * 2006-09-08 2016-09-28 Ambrx公司 用于脊椎动物细胞的杂合抑制tRNA
AU2008210538B2 (en) 2007-01-31 2013-06-06 Pelican Technology Holdings, Inc. Bacterial leader sequences for increased expression
PL2068909T3 (pl) 2007-03-30 2012-09-28 Ambrx Inc Modyfikowane polipeptydy fgf-21 i ich zastosowanie
AU2008279584A1 (en) * 2007-04-27 2009-01-29 Dow Global Technologies Inc. Improved production and in vivo assembly of soluble recombinant icosahedral virus-like particles
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
JP5444553B2 (ja) 2007-04-27 2014-03-19 フェネックス インコーポレイテッド 微生物宿主を迅速にスクリーニングして、異種タンパク質発現の収率および／または質が改善されている特定の株を同定する方法
EP2076533B1 (en) * 2007-05-02 2014-10-08 Ambrx, Inc. Modified interferon beta polypeptides and their uses
ES2632504T3 (es) * 2007-11-20 2017-09-13 Ambrx, Inc. Polipéptidos de insulina modificados y sus usos
WO2009124012A1 (en) * 2008-03-31 2009-10-08 Dow Global Technologies Inc. A design for rapidly cloning one or more polypeptide chains into an expression system
JP5680534B2 (ja) * 2008-07-23 2015-03-04 イーライ リリー アンド カンパニー 修飾されているウシｇ−ｃｓｆポリペプチドおよびそれらの使用
PE20110832A1 (es) 2008-09-26 2011-11-24 Ambrx Inc Polipeptidos de eritropoyetina felina modificados
MX348657B (es) 2008-09-26 2017-06-21 Ambrx Inc Microorganismos y vacunas dependientes de replicacion de aminoacidos no naturales.
EP2430164B1 (en) 2009-05-11 2017-07-19 Pfenex, Inc. Production of recombinant proteins utilizing non-antibiotic selection methods and the incorporation of non-natural amino acids therein
WO2011109556A2 (en) * 2010-03-04 2011-09-09 Pfenex Inc. Method for producing soluble recombinant interferon protein without denaturing
CA2793978C (en) 2010-03-30 2021-08-03 Pfenex Inc. High level expression of recombinant toxin proteins
WO2011123570A2 (en) 2010-04-01 2011-10-06 Pfenex Inc. Methods for g-csf production in a pseudomonas host cell
US9567386B2 (en) 2010-08-17 2017-02-14 Ambrx, Inc. Therapeutic uses of modified relaxin polypeptides
CA2808596C (en) 2010-08-17 2020-12-15 Ambrx, Inc. Modified relaxin polypeptides and their uses
AR083006A1 (es) 2010-09-23 2013-01-23 Lilly Co Eli Formulaciones para el factor estimulante de colonias de granulocitos (g-csf) bovino y variantes de las mismas
US9169304B2 (en) 2012-05-01 2015-10-27 Pfenex Inc. Process for purifying recombinant Plasmodium falciparum circumsporozoite protein
JP6702962B2 (ja) 2014-10-24 2020-06-03 ブリストル−マイヤーズ スクイブ カンパニーＢｒｉｓｔｏｌ−Ｍｙｅｒｓ Ｓｑｕｉｂｂ Ｃｏｍｐａｎｙ 改変ｆｇｆ−２１ポリペプチドおよびその使用
EA035117B1 (ru) 2014-11-20 2020-04-29 Байолоджикал И Лимитед Кодон-оптимизированный полинуклеотид для экспрессии crmна высоком уровне
DK3227455T3 (da) 2014-12-01 2023-08-21 Pfenex Inc Fusionspartnere til peptidfremstilling
CN110637027A (zh) 2017-02-08 2019-12-31 百时美施贵宝公司 包含药代动力学增强子的修饰的松弛素多肽及其用途
AU2018354067B2 (en) 2017-10-27 2023-03-30 Pfenex Inc. Method for production of recombinant Erwinia asparaginase
AU2018354069A1 (en) 2017-10-27 2020-06-11 Pelican Technology Holdings, Inc. Bacterial leader sequences for periplasmic protein expression
EP4267592A1 (en) 2020-12-23 2023-11-01 Jazz Pharmaceuticals Ireland Ltd. Methods of purifying charge-shielded fusion proteins
WO2022211829A1 (en) 2021-03-30 2022-10-06 Jazz Pharmaceuticals Ireland Ltd. Dosing of recombinant l-asparaginase

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE52036B1 (en) * 1979-05-24 1987-05-27 Univ California Non-passageable viruses
GR68404B (ja) * 1979-06-01 1981-12-29 Univ California
US4342832A (en) * 1979-07-05 1982-08-03 Genentech, Inc. Method of constructing a replicable cloning vehicle having quasi-synthetic genes
US4338397A (en) * 1980-04-11 1982-07-06 President And Fellows Of Harvard College Mature protein synthesis
IE52755B1 (en) * 1980-08-26 1988-02-17 Univ California Bovine pre-growth and growth hormone
FI64813C (fi) * 1980-12-31 1984-01-10 Ilkka Antero Palva Foerfarande foer producering av ett utvalt aeggviteaemne och vid foerfarandet anvaenda rekombinantplasmidvektorer
DK582381A (da) * 1981-01-02 1982-07-03 Univ New York Remkombinat plasmid til anvendelse ved bakteriel kloning
US4775622A (en) * 1982-03-08 1988-10-04 Genentech, Inc. Expression, processing and secretion of heterologous protein by yeast
EP0095361B1 (en) * 1982-05-25 1989-07-26 Eli Lilly And Company Cloning vectors for expression of exogenous protein
JPS59135892A (ja) * 1983-01-25 1984-08-04 Kunio Yamane シグナルアミノ酸配列をコードするｄｎａ

Also Published As

Publication number Publication date
DK204684D0 (da) 1984-04-24
US4755465A (en) 1988-07-05
AU2721984A (en) 1984-11-01
NZ207924A (en) 1988-03-30
CA1267099A (en) 1990-03-27
EP0127305A1 (en) 1984-12-05
JPS6045595A (ja) 1985-03-12
IL71622A0 (en) 1984-07-31
AU586609B2 (en) 1989-07-20
ES8602136A1 (es) 1985-12-01
ES531896A0 (es) 1985-12-01
ZA843062B (en) 1984-11-28
JPH05252936A (ja) 1993-10-05
IL71622A (en) 1990-03-19

Similar Documents

Publication Publication Date Title
DK204684A (da) Humant vaeksthormon frit for andre proteiner forbundet med dets native miljoe, udtrykkelsesmedium, som kan frembringe produktion af prae-hgh i en prokaryotisk vaertscelle, prokaryotisk vaert transformeret dermed og fremgangsmaade til fremstilling af modent hgh
DK0574402T3 (da) Ekspression af PACE i værtsceller og fremgangsmåder til anvendelse deraf
PT87394A (pt) Process for plant tissue transformation
DK198883A (da) Fremgangsmaade til fremstilling af menneskevaevs-plasminogen-aktivator, dna-sekvens der koder for denne aktivator, replicerbar udtrykkelsesvektor for dna-sekvensen og mikroorganisme eller cellekultur transformeret med udtrykkelsesvektoren
ES8700868A1 (es) Metodo de aislamiento de un fragmento de gen que codifica por lo menos una porcion de una proteina.
IT1095541B (it) Protesi di valvola cardiaca e metodo per la sua produzione
IT1125368B (it) Metodo per la produzione di corpi di vetro,in particolare filamenti ottici
IL83192A (en) Method for the preparation of proteins with factor viii activity by microbial host cells;expression vectors,host cells,antibodies
DK473987D0 (da) Fremgangsmaade til kontinuert produktion af et oensket heterologt protein i en eukaryotisk vaertcelle, vektor, som tilfoerer denne evne, og celler transformeret med vektoren
DE69130981D1 (de) Expression von makrophagen-induzierbaren proteinen (mips) in hefezellen
EP0182448A3 (en) Production of factor viii and related products
NO863535L (no) Linseholder, saerlig holder for brilleglass som skal rengjoeres og deretter paafoeres belegg.
DK133780A (da) Fremgangsmaade til fremstilling af interferon
DK262784D0 (da) Mikrobiologisk fremstillet d-2-hydroxy-4-methylpentansyredehydrogenase, fremgangsmaade til dets udvinding og dets anvendelse
IL89242A (en) Process for producing a recombinant microorganism capable of converting glucose into 2-keto-l-gulonic acid,the microorganism obtained by said process and its use in converting glucose into 2-keto-l-gulonic acid
GB2151610B (en) Hollow glass shell microcarrier for growth of cell cultures, and method of shell manufacture
RO91666A (ro) Procedeu pentru prepararea unor derivati de acid fenoxiprostatrienoic
DE300666T1 (de) Gefaess fuer zellkultur im batch-verfahren.
DK428184D0 (da) 2-oxocarbonsyrereduktase, fremgangsmaade til fremstilling heraf samt anvendelse heraf til reduktion af 2-oxocarbonsyrer og disses estere
DK163195C (da) Fremgangsmaade til radioaktiv maerkning af blodceller, oploesning til brug ved fremgangsmaaden, fremgangsmaade til fremstilling af oploesningen, og saet til radioaktiv maerkning af blodceller
JPS5712996A (en) Glucose isomerase , production and utilization thereof
IT8119533A0 (it) Metodo per la produzione di isomerasi del glucosio immobilizzata.
DK285587D0 (da) Ekspressionsvektor med evne til inducibar ekspression af et fremmed strukturgen, der baeres af ekspressionsvektoren, i vaertsceller og fremgangsmaade tilfremstilling af oenskede genprodukter
DK282387D0 (da) Sammensat dna-sekvens og dennes anvendelse til fremstilling af vaeksthormon
JPS553796A (en) Plant supported by aqueous gel and plant growing method

Legal Events

Date Code Title Description
AHS Application shelved for other reasons than non-payment