ES8700868A1 - Metodo de aislamiento de un fragmento de gen que codifica por lo menos una porcion de una proteina. - Google Patents

Metodo de aislamiento de un fragmento de gen que codifica por lo menos una porcion de una proteina.

Info

Publication number
ES8700868A1
ES8700868A1 ES537146A ES537146A ES8700868A1 ES 8700868 A1 ES8700868 A1 ES 8700868A1 ES 537146 A ES537146 A ES 537146A ES 537146 A ES537146 A ES 537146A ES 8700868 A1 ES8700868 A1 ES 8700868A1
Authority
ES
Spain
Prior art keywords
factor viii
production
related products
obtaining
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES537146A
Other languages
English (en)
Other versions
ES537146A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genetics Institute LLC
Original Assignee
Genetics Institute LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27068312&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES8700868(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from US06/546,650 external-priority patent/US4757006A/en
Application filed by Genetics Institute LLC filed Critical Genetics Institute LLC
Publication of ES537146A0 publication Critical patent/ES537146A0/es
Publication of ES8700868A1 publication Critical patent/ES8700868A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Diabetes (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Toxicology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

METODO DE AISLAMIENTO D EUN FRAGMENTO DE GEN QUE CODIFICA POR LO MENOS UNA POSCION DE UNA PROTEINA. USANDO TECNICAS DE MANIPULACION DE GENES SE HA DESARROLLADO UN METODO QUE PERMITE LA PRODUCCION DE AHF MEDIANTE IDENTIFICACION Y CLONACION DEL GEN QUE COFDIFICA LA PROTEINA AHF HUMANA, CLONACION DE DICHO GEN, INCORPORACION DE ESTE EN UN VECTOR DE ADN RECOMBINANTE, TRANSFORMACION DE UN HUESPED ADECUADO CON EL VECTOR QUE INCLUYE EL CITADO GEN EXPRESION DEL GEN AHF HUMANO PRODUCIDO. ANALOGAMENTE SE PUEDE PRODUCIR AHF PORCINO. EL METODO PERMITE PRODUCIR <AHF> HUMANO MEDIANTE LAS TECNICAS DE ADN RECOMBINANTE QUE CODIFICA LA PRODUCCION CELULAR DE FACTOR VIII:C HUMANO DEFICITARIA O AUSENTE EN PACIENTES CON HEMOFILIA A.
ES537146A 1983-10-28 1984-10-26 Metodo de aislamiento de un fragmento de gen que codifica por lo menos una porcion de una proteina. Expired ES8700868A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US06/546,650 US4757006A (en) 1983-10-28 1983-10-28 Human factor VIII:C gene and recombinant methods for production
US64403684A 1984-08-24 1984-08-24

Publications (2)

Publication Number Publication Date
ES537146A0 ES537146A0 (es) 1986-10-16
ES8700868A1 true ES8700868A1 (es) 1986-10-16

Family

ID=27068312

Family Applications (4)

Application Number Title Priority Date Filing Date
ES537146A Expired ES8700868A1 (es) 1983-10-28 1984-10-26 Metodo de aislamiento de un fragmento de gen que codifica por lo menos una porcion de una proteina.
ES546653A Expired ES8800987A1 (es) 1983-10-28 1985-09-02 Metodo de produccion del factor viii:c humano
ES546655A Expired ES8704546A1 (es) 1983-10-28 1985-09-02 Metodo de produccion de una proteina con actividad procoagu-lante del tipo de factor viii:c humano
ES546654A Expired ES8800359A1 (es) 1983-10-28 1985-09-02 Metodo de produccion de factor viii:c humano

Family Applications After (3)

Application Number Title Priority Date Filing Date
ES546653A Expired ES8800987A1 (es) 1983-10-28 1985-09-02 Metodo de produccion del factor viii:c humano
ES546655A Expired ES8704546A1 (es) 1983-10-28 1985-09-02 Metodo de produccion de una proteina con actividad procoagu-lante del tipo de factor viii:c humano
ES546654A Expired ES8800359A1 (es) 1983-10-28 1985-09-02 Metodo de produccion de factor viii:c humano

Country Status (9)

Country Link
EP (1) EP0162067B1 (es)
JP (4) JPH07106156B2 (es)
DK (1) DK174927B1 (es)
ES (4) ES8700868A1 (es)
FI (1) FI86436C (es)
IE (1) IE57884B1 (es)
IT (1) IT1177066B (es)
MX (1) MX9203437A (es)
WO (1) WO1985001961A1 (es)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4703008A (en) * 1983-12-13 1987-10-27 Kiren-Amgen, Inc. DNA sequences encoding erythropoietin
NZ210501A (en) * 1983-12-13 1991-08-27 Kirin Amgen Inc Erythropoietin produced by procaryotic or eucaryotic expression of an exogenous dna sequence
KR850004274A (ko) * 1983-12-13 1985-07-11 원본미기재 에리트로포이에틴의 제조방법
DE122004000028I1 (de) * 1984-01-12 2004-09-30 Chiron Corp F}r Faktor-VIIIc kodierende DNA-Sequenzen und verwandte DNA-Konstruktionen.
US5045455A (en) * 1984-01-12 1991-09-03 Chiron Corporation Factor VIII:C cDNA cloning and expression
US5004804A (en) * 1984-01-12 1991-04-02 Nordisk Gentofte Method and composition for preparation of factor VIIIC
US4716117A (en) * 1984-10-26 1987-12-29 Chiron Corporation Monoclonal antibodies to factor VIIIC
ZA852099B (en) * 1984-03-26 1985-12-24 Meloy Lab Recombinant factor viii-c.
US4965199A (en) * 1984-04-20 1990-10-23 Genentech, Inc. Preparation of functional human factor VIII in mammalian cells using methotrexate based selection
FI86885C (fi) * 1984-04-20 1992-10-26 Genentech Inc Foerfarande foer framstaellning av human rekombinantfaktor viii och nukleinsyrasekvenser och vektorer anvaend daertill
EP0182448A3 (en) * 1984-08-24 1987-10-28 Genetics Institute, Inc. Production of factor viii and related products
EP0218692A4 (en) * 1985-04-11 1988-03-22 Childrens Medical Center VON WILLEBRAND FACTOR.
US8597910B1 (en) 1985-04-11 2013-12-03 Children's Medical Center Corporation DNA encoding Von Willebrand Factor (VWF) and methods and cells for producing VFW, and VFW produced by the DNA, methods and cells
DE3531360A1 (de) * 1985-09-03 1987-05-07 Merck Patent Gmbh Dna-sequenzen, rekombinante dna-molekuele und verfahren zur herstellung des enzyms mutarotase aus acinetobacter calcoaceticus
WO1987004187A1 (en) * 1986-01-03 1987-07-16 Genetics Institute, Inc. METHOD FOR PRODUCING FACTOR VIII:c-TYPE PROTEINS
US5250421A (en) * 1986-01-03 1993-10-05 Genetics Institute, Inc. Method for producing factor VIII:C-type proteins
US5198349A (en) * 1986-01-03 1993-03-30 Genetics Institute, Inc. Method for producing factor VIII:C and analogs
US5595886A (en) * 1986-01-27 1997-01-21 Chiron Corporation Protein complexes having Factor VIII:C activity and production thereof
FR2599754B1 (fr) * 1986-06-06 1989-12-29 Transgene Sa Procede de preparation de facteur viii a partir de cellules de mammiferes
DE3769872D1 (de) 1986-07-11 1991-06-13 Miles Inc Herstellung von rekombinantem protein.
EP0302925A4 (en) * 1987-01-30 1989-04-24 Biogen Nv METHOD FOR PRODUCING FACTOR VIII IN LARGE QUANTITIES.
CA1331157C (en) * 1987-04-06 1994-08-02 Randal J. Kaufman Method for producing factor viii:c-type proteins
US6060447A (en) * 1987-05-19 2000-05-09 Chiron Corporation Protein complexes having Factor VIII:C activity and production thereof
EP0294910B1 (en) 1987-06-12 1996-09-11 Immuno Ag Novel proteins with factor VIII activity, process for their preparation using genetically engineered cells and pharmaceutical compositions containing them
DK0500734T3 (da) * 1989-11-17 1998-03-30 Chiron Corp Proteinkomplekser med faktor VIII:C-aktivitet samt frembringelse deraf
US5155218A (en) 1990-05-08 1992-10-13 Neurogenetic Corporation Dna encoding human 5-ht1d receptors
DK53792D0 (da) * 1992-04-24 1992-04-24 Novo Nordisk As Fremgangsmaade til fremstilling af proteiner
US5563045A (en) * 1992-11-13 1996-10-08 Genetics Institute, Inc. Chimeric procoagulant proteins
US5910481A (en) * 1995-11-13 1999-06-08 Immuno Ag Hybrid proteins with modified activity
US6200560B1 (en) 1998-10-20 2001-03-13 Avigen, Inc. Adeno-associated virus vectors for expression of factor VIII by target cells
US6221349B1 (en) 1998-10-20 2001-04-24 Avigen, Inc. Adeno-associated vectors for expression of factor VIII by target cells
AU2004278684B2 (en) 2003-09-30 2011-05-12 The Trustees Of The University Of Pennsylvania Adeno-associated virus (AAV) clades, sequences, vectors containing same, and uses therefor
WO2005123928A1 (en) * 2004-06-08 2005-12-29 Battelle Memorial Institute Production of human coagulation factor viii from plant cells and whole plants
CN112029737A (zh) 2005-04-07 2020-12-04 宾夕法尼亚大学托管会 增强腺相关病毒载体功能的方法
US9315825B2 (en) 2010-03-29 2016-04-19 The Trustees Of The University Of Pennsylvania Pharmacologically induced transgene ablation system
CN102869779A (zh) 2010-03-29 2013-01-09 宾夕法尼亚大学托管会 药理学诱导的转基因消融系统
WO2012112832A1 (en) 2011-02-17 2012-08-23 The Trustees Of The University Of Pennsylvania Compositions and methods for altering tissue specificity and improving aav9-mediated gene transfer
WO2015012924A2 (en) 2013-04-29 2015-01-29 The Trustees Of The University Of Pennsylvania Tissue preferential codon modified expression cassettes, vectors containing same, and use thereof
US10058624B2 (en) 2015-04-16 2018-08-28 Emory University Recombinant promoters and vectors for protein expression in liver and use thereof
WO2022032140A2 (en) 2020-08-07 2022-02-10 Amicus Therapeutics, Inc. Vesicle targeting proteins and uses of same

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US602312A (en) * 1898-04-12 eades
US4361509A (en) * 1981-12-14 1982-11-30 Scripps Clinic And Research Foundation Ultrapurification of factor VIII using monoclonal antibodies
US4663280A (en) * 1983-05-19 1987-05-05 Public Health Research Institute Of The City Of New York Expression and secretion vectors and method of constructing vectors
NZ208612A (en) * 1983-06-24 1991-09-25 Genentech Inc Method of producing "procaryotic carbonyl hydrolases" containing predetermined, site specific mutations
DE122004000028I1 (de) * 1984-01-12 2004-09-30 Chiron Corp F}r Faktor-VIIIc kodierende DNA-Sequenzen und verwandte DNA-Konstruktionen.
ZA852099B (en) * 1984-03-26 1985-12-24 Meloy Lab Recombinant factor viii-c.
FI86885C (fi) * 1984-04-20 1992-10-26 Genentech Inc Foerfarande foer framstaellning av human rekombinantfaktor viii och nukleinsyrasekvenser och vektorer anvaend daertill

Also Published As

Publication number Publication date
JPH02182188A (ja) 1990-07-16
DK174927B1 (da) 2004-03-01
ES537146A0 (es) 1986-10-16
JP2549049B2 (ja) 1996-10-30
EP0162067A1 (en) 1985-11-27
FI852547L (fi) 1985-06-27
DK292385D0 (da) 1985-06-27
FI86436B (fi) 1992-05-15
JPS61500646A (ja) 1986-04-10
ES546654A0 (es) 1987-10-16
ES8704546A1 (es) 1987-04-01
JPH0690763A (ja) 1994-04-05
IT1177066B (it) 1987-08-26
FI86436C (fi) 1992-08-25
IE57884B1 (en) 1993-05-05
EP0162067A4 (en) 1988-03-22
JPH07106156B2 (ja) 1995-11-15
ES8800987A1 (es) 1987-12-16
DK292385A (da) 1985-06-27
ES546653A0 (es) 1987-12-16
ES8800359A1 (es) 1987-10-16
ES546655A0 (es) 1987-04-01
JPH02227078A (ja) 1990-09-10
MX9203437A (es) 1992-07-01
FI852547A0 (fi) 1985-06-27
EP0162067B1 (en) 1992-07-15
WO1985001961A1 (en) 1985-05-09
IT8423351A0 (it) 1984-10-26

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