DK1507002T3 - Termostabilt Taq-polymerasefragment - Google Patents

Termostabilt Taq-polymerasefragment

Info

Publication number
DK1507002T3
DK1507002T3 DK04018913T DK04018913T DK1507002T3 DK 1507002 T3 DK1507002 T3 DK 1507002T3 DK 04018913 T DK04018913 T DK 04018913T DK 04018913 T DK04018913 T DK 04018913T DK 1507002 T3 DK1507002 T3 DK 1507002T3
Authority
DK
Denmark
Prior art keywords
delta
taq
taqwt
nucleic acid
well
Prior art date
Application number
DK04018913T
Other languages
Danish (da)
English (en)
Inventor
Waltraud Dr Ankenbauer
Thomas Dr Meier
Annette Dr Deufel
Dieter Dr Heindl
Gisela Dr Betzl
Rainer Dr Schmuck
Bernd Dr Schneidinger
Jessica Strey
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of DK1507002T3 publication Critical patent/DK1507002T3/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • C12N9/1252DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
DK04018913T 2003-08-12 2004-08-10 Termostabilt Taq-polymerasefragment DK1507002T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP03017636 2003-08-12

Publications (1)

Publication Number Publication Date
DK1507002T3 true DK1507002T3 (da) 2007-04-10

Family

ID=34130067

Family Applications (1)

Application Number Title Priority Date Filing Date
DK04018913T DK1507002T3 (da) 2003-08-12 2004-08-10 Termostabilt Taq-polymerasefragment

Country Status (15)

Country Link
US (1) US7972830B2 (zh)
EP (1) EP1507002B1 (zh)
JP (1) JP3929061B2 (zh)
KR (1) KR100626578B1 (zh)
CN (1) CN1590543B (zh)
AT (1) ATE348881T1 (zh)
AU (1) AU2004203649B2 (zh)
BR (1) BRPI0403221A (zh)
CA (1) CA2474734C (zh)
DE (1) DE602004003755T2 (zh)
DK (1) DK1507002T3 (zh)
ES (1) ES2278249T3 (zh)
HK (1) HK1074458A1 (zh)
MX (1) MXPA04007721A (zh)
SG (1) SG109552A1 (zh)

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WO2006074217A2 (en) * 2005-01-04 2006-07-13 Stratagene California Hot start polymerase reaction using a thermolabile blocker
DE102006025154A1 (de) * 2006-05-30 2007-12-06 Universität Konstanz Mutierte DNA-Polymerase mit erhöhter Reverse Transkriptase Aktivität
EP2113574A1 (en) 2008-04-28 2009-11-04 Biotype AG Substances and methods for a DNA based profiling assay
US20140255925A9 (en) * 2008-04-30 2014-09-11 Integrated Dna Technologies Modified rnase h enzymes and their uses
US20090325169A1 (en) 2008-04-30 2009-12-31 Integrated Dna Technologies, Inc. Rnase h-based assays utilizing modified rna monomers
WO2010062777A2 (en) * 2008-11-03 2010-06-03 Kapabiosystems Modified type a dna polymerases
DK2501716T3 (en) * 2009-11-19 2015-04-07 Solis Biodyne Oü Formations to increase polypeptidstabilitet and activity and related practices
US20110250598A1 (en) 2010-04-12 2011-10-13 Ulrike Fischer Detergent free polymerases
US9315787B2 (en) 2011-01-14 2016-04-19 Kapa Biosystems, Inc. Modified DNA polymerases for improved amplification
WO2012135053A2 (en) 2011-03-25 2012-10-04 Integrated Dna Technologies, Inc. Rnase h-based assays utilizing modified rna monomers
WO2012146260A1 (de) 2011-04-23 2012-11-01 Biolytix Ag Herstellung und verwendung von proteinen in der molekularbiologie
US8980333B2 (en) 2011-06-08 2015-03-17 Life Technologies Corporation Development of novel detergents for use in PCR systems
WO2012170907A2 (en) 2011-06-08 2012-12-13 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
JP6742238B2 (ja) 2013-10-25 2020-08-19 ライフ テクノロジーズ コーポレーション Pcrシステムにおいて使用するための新規化合物及びその用途
EP3234115B1 (en) * 2014-12-16 2020-07-22 Life Technologies Corporation Polymerase compositions and methods of making and using same
JP6822974B2 (ja) 2015-05-29 2021-01-27 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft シトラコン酸無水物によって微生物を不活性化する方法
CN108473970B (zh) * 2015-11-27 2022-09-13 国立大学法人九州大学 Dna聚合酶变体
GB201604876D0 (en) * 2016-03-22 2016-05-04 Uni I Tromsø Norges Arktiske Uni Marine DNA polymerases
CN108265039B (zh) * 2016-12-30 2020-07-14 天津强微特生物科技有限公司 一种突变TaqDNA聚合酶及其纯化方法
US20200181666A1 (en) * 2017-03-29 2020-06-11 Editas Medicine, Inc. Nucleic acid mutagenesis methods
WO2018209092A1 (en) * 2017-05-10 2018-11-15 Board Of Regents, The University Of Texas System Methods and devices related to amplifying nucleic acid at a variety of temperatures
WO2019143622A1 (en) * 2018-01-19 2019-07-25 Bio-Rad Laboratories, Inc. Mutant dna polymerases
GB201812428D0 (en) * 2018-07-31 2018-09-12 Bg Res Ltd Improved method for pathogen detection
CN115836133A (zh) 2020-04-30 2023-03-21 萨达维达生物科学研究与服务有限公司 用于检测疑似冠状病毒样品中的核酸序列的方法和便携式装置
US11649441B2 (en) * 2020-10-07 2023-05-16 Abclonal Science, Inc. Taq DNA polymerase mutants for probe qPCR
WO2023122291A2 (en) * 2021-12-23 2023-06-29 Sherlock Biosciences, Inc. Methods for polypeptide purification
CN116024320A (zh) * 2022-07-13 2023-04-28 上海翔琼生物技术有限公司 一种用于检测核酸的荧光定量pcr方法

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GB9920194D0 (en) 1999-08-27 1999-10-27 Advanced Biotech Ltd A heat-stable thermostable DNA polymerase for use in nucleic acid amplification
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CA2409775C (en) 2001-12-03 2010-07-13 F. Hoffmann-La Roche Ag Reversibly modified thermostable enzymes for dna synthesis and amplification in vitro

Also Published As

Publication number Publication date
US7972830B2 (en) 2011-07-05
SG109552A1 (en) 2005-03-30
DE602004003755D1 (de) 2007-02-01
CA2474734C (en) 2010-07-13
ES2278249T3 (es) 2007-08-01
US20050037412A1 (en) 2005-02-17
DE602004003755T2 (de) 2007-10-11
BRPI0403221A (pt) 2005-07-12
AU2004203649A1 (en) 2005-03-03
KR20050025266A (ko) 2005-03-14
CN1590543A (zh) 2005-03-09
EP1507002B1 (en) 2006-12-20
EP1507002A3 (en) 2005-07-27
KR100626578B1 (ko) 2006-09-25
JP3929061B2 (ja) 2007-06-13
CN1590543B (zh) 2010-05-12
EP1507002A2 (en) 2005-02-16
CA2474734A1 (en) 2005-02-12
HK1074458A1 (en) 2005-11-11
JP2005058236A (ja) 2005-03-10
MXPA04007721A (es) 2005-07-12
ATE348881T1 (de) 2007-01-15
AU2004203649B2 (en) 2006-01-12

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