DK1350852T3 - Fremgangsmåde til hurtig typning af mikroorganismer ved hjælp af pulsed-field gelelektroforese, hvorved pröverne fremstilles i en autoklaverbar og fleksibel form - Google Patents

Fremgangsmåde til hurtig typning af mikroorganismer ved hjælp af pulsed-field gelelektroforese, hvorved pröverne fremstilles i en autoklaverbar og fleksibel form

Info

Publication number
DK1350852T3
DK1350852T3 DK01983428T DK01983428T DK1350852T3 DK 1350852 T3 DK1350852 T3 DK 1350852T3 DK 01983428 T DK01983428 T DK 01983428T DK 01983428 T DK01983428 T DK 01983428T DK 1350852 T3 DK1350852 T3 DK 1350852T3
Authority
DK
Denmark
Prior art keywords
gel electrophoresis
electrophoresis
pulsed
aid
autoclavable
Prior art date
Application number
DK01983428T
Other languages
Danish (da)
English (en)
Inventor
Canovas Lilia Lopez
Rojas Ana Maria Riveron
Alonso Axel Sanchez
Donderiz Hilda Teresa Clark
Perez Regnar Gigato
Diaz Oscar Arencibia
Clarke David Higgison
Orozco Esther Orozco
Acu A Maria Concepcion Ariosa
Original Assignee
Cnic Ct Nac Investigaciones
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cnic Ct Nac Investigaciones filed Critical Cnic Ct Nac Investigaciones
Application granted granted Critical
Publication of DK1350852T3 publication Critical patent/DK1350852T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Electrochemistry (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
DK01983428T 2000-11-07 2001-11-02 Fremgangsmåde til hurtig typning af mikroorganismer ved hjælp af pulsed-field gelelektroforese, hvorved pröverne fremstilles i en autoklaverbar og fleksibel form DK1350852T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CU20000247A CU23095A1 (es) 2000-11-07 2000-11-07 Proceso para la tipificación rápida de microorganismos y juego de reactivos empleado
PCT/CU2001/000008 WO2002038802A1 (es) 2000-11-07 2001-11-02 Proceso para la tipificación rápida de microorganismos y juego de reactivos empleado

Publications (1)

Publication Number Publication Date
DK1350852T3 true DK1350852T3 (da) 2007-05-07

Family

ID=40134841

Family Applications (1)

Application Number Title Priority Date Filing Date
DK01983428T DK1350852T3 (da) 2000-11-07 2001-11-02 Fremgangsmåde til hurtig typning af mikroorganismer ved hjælp af pulsed-field gelelektroforese, hvorved pröverne fremstilles i en autoklaverbar og fleksibel form

Country Status (19)

Country Link
US (1) US7731828B2 (de)
EP (1) EP1350852B1 (de)
JP (1) JP3955817B2 (de)
CN (1) CN100532569C (de)
AT (1) ATE350490T1 (de)
AU (1) AU2002214939A1 (de)
BR (1) BR0115172B1 (de)
CA (1) CA2428313C (de)
CU (1) CU23095A1 (de)
CY (1) CY1106369T1 (de)
DE (1) DE60125832T2 (de)
DK (1) DK1350852T3 (de)
EC (1) ECSP034577A (de)
ES (1) ES2276843T3 (de)
HK (1) HK1063822A1 (de)
MX (1) MXPA03004006A (de)
PT (1) PT1350852E (de)
WO (1) WO2002038802A1 (de)
ZA (1) ZA200303472B (de)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7482116B2 (en) 2002-06-07 2009-01-27 Dna Genotek Inc. Compositions and methods for obtaining nucleic acids from sputum
CA2418582A1 (en) * 2003-02-07 2004-08-07 Dupont Canada Inc. A method for simulating and modeling the presence and growth of microbes, including pathogens and spoilage organisms through a food supply chain
US20080079726A1 (en) * 2006-07-03 2008-04-03 Wolfgang Geiger Visual display of process sequences
US20080044822A1 (en) * 2006-08-21 2008-02-21 Gafur Zainiev Nucleic acid array with releaseable nucleic acid probes
US20080044821A1 (en) * 2006-08-21 2008-02-21 Gafur Zainiev Nucleic acid array having fixed nucleic acid anti-probes and complementary free nucleic acid probes
US20100056388A1 (en) * 2006-08-21 2010-03-04 Cnvgenes, Inc. Nucleic acid array having fixed nucleic acid anti-probes and complementary free nucleic acid probes
US20090286694A1 (en) * 2006-08-21 2009-11-19 Gafur Zainiev Nucleic acid array with releaseable nucleic acid probes
WO2012170560A2 (en) 2011-06-06 2012-12-13 Cornell University Microfluidic device for extracting, isolating, and analyzing dna from cells
EP3928715A1 (de) 2011-06-19 2021-12-29 DNA Genotek, Inc. Vorrichtungen, lösungen und verfahren zur probenentnahme
CN103196980B (zh) * 2013-03-27 2014-10-08 中国水产科学研究院淡水渔业研究中心 鱼源链球菌脉冲场凝胶电泳快速分型方法
US9999856B2 (en) 2013-07-26 2018-06-19 General Electric Company Methods for electroelution of biomolecules
US9333463B2 (en) 2013-07-26 2016-05-10 General Electric Company Devices and systems for elution of biomolecules
CA2941764C (en) * 2014-03-07 2023-10-24 Dna Genotek Inc. Composition and method for stabilizing nucleic acids in biological samples
CN105372318B (zh) * 2014-08-28 2018-01-05 山东省肿瘤医院 将细胞原位电泳与流式分析术结合的细胞固定方法
CN104561326B (zh) * 2015-01-16 2017-02-22 天津医科大学 一种基于菌毛多样性的尿路感染大肠杆菌的分型方法
US11383240B2 (en) 2016-05-22 2022-07-12 Cornell University Single cell whole genome amplification via micropillar arrays under flow conditions
WO2018051374A1 (en) 2016-09-15 2018-03-22 Dianax S.R.L. Microfluidic device, particularly of the lab-on-chip type, for the concentration, purification, and detection of biological and/or medical targets of interest from a biological sample
KR102388753B1 (ko) * 2020-07-20 2022-04-20 광주과학기술원 유전영동 힘을 이용한 미생물 검출 장치

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4707237A (en) * 1982-09-09 1987-11-17 Ciba Corning Diagnostics Corp. System for identification of cells by electrophoresis
US4861448A (en) * 1982-11-18 1989-08-29 The Trustees Of Columbia University In The City Of New York Electrophoretic methods employing gel inserts
US4473452A (en) * 1982-11-18 1984-09-25 The Trustees Of Columbia University In The City Of New York Electrophoresis using alternating transverse electric fields
CA2052037A1 (en) * 1990-10-02 1992-04-03 Eric Lai Automatic control system for improved electrophoresis
FR2693209B1 (fr) * 1992-07-03 1994-09-30 Inst Nat Sante Rech Med Procédé de détermination de la taille en nucléotides de fragments d'ADN.
KR100230909B1 (ko) 1993-11-29 1999-12-01 다니엘 엘. 캐시앙 광범위의 유기체로부터 핵산 추출 방법
DE69924900T2 (de) * 1998-02-16 2006-02-23 Mallinckrodt, Inc. Behälter für ein elektroforetisches Gel und zugehöriges Verwendungsverfahren

Also Published As

Publication number Publication date
EP1350852A1 (de) 2003-10-08
PT1350852E (pt) 2007-03-30
CA2428313C (en) 2010-09-14
AU2002214939A1 (en) 2002-05-21
CU23095A1 (es) 2005-11-18
DE60125832T2 (de) 2007-10-11
CA2428313A1 (en) 2002-05-16
EP1350852B1 (de) 2007-01-03
CN100532569C (zh) 2009-08-26
JP3955817B2 (ja) 2007-08-08
US20040050700A1 (en) 2004-03-18
JP2004513375A (ja) 2004-04-30
ECSP034577A (es) 2003-08-29
BR0115172A (pt) 2004-02-17
CY1106369T1 (el) 2011-10-12
ATE350490T1 (de) 2007-01-15
WO2002038802A1 (es) 2002-05-16
BR0115172B1 (pt) 2014-10-21
CN1479790A (zh) 2004-03-03
MXPA03004006A (es) 2004-04-02
ZA200303472B (en) 2003-11-11
ES2276843T3 (es) 2007-07-01
US7731828B2 (en) 2010-06-08
DE60125832D1 (de) 2007-02-15
HK1063822A1 (en) 2005-01-14

Similar Documents

Publication Publication Date Title
DK1350852T3 (da) Fremgangsmåde til hurtig typning af mikroorganismer ved hjælp af pulsed-field gelelektroforese, hvorved pröverne fremstilles i en autoklaverbar og fleksibel form
DE69818650D1 (de) Gerät und methoden zum einfnag und zur analyse von partikel-einheiten
DE69939945D1 (de) Verfahren zur isolierung von stammzellen
DK1157264T3 (da) Biokemiske oprensningsindretninger med immobiliserede opfangningsprober og anvendelser deraf
DE19882221T1 (de) Verfahren und Vorrichtung zum automatischen Impfen von Kulturmedien mit bakteriellen Proben aus klinischen Probenbehältern
DE60233142D1 (de) Verfahren und reagentien zum nachweis von endotoxin
ATE178098T1 (de) Vorrichtung zur dürchführung von magnetischen zyklusreactionen
ATE337855T1 (de) Verfahren und vorrichtung zur vorbehandlung von proben durch zentrifugieren
ATE258683T1 (de) Verfahren zur durchführung einer speichelanalyse
ATE161041T1 (de) Verfahren zur hcv-züchtung in zell-linien aus b- oder t-lymphozyten
DE59000762D1 (de) Verfahren zur schnellen detektion von mikroorganismen in proben und vorrichtung zur durchfuehrung des verfahrens.
DE69434117D1 (de) Verfahren zur typisierung von hepatitis c viren und dafür zu verwendende reagenzien
WO2002042500A3 (en) Apparatus and method for electrophoretic microspot concentration
HK1085010A1 (en) Latex reagent for adiponectin analysis and method of adiponectin analysis
ATE445835T1 (de) Elektrophorese-kammer in pulsierendem feld, zubehör und verfahren zur verwendung für die trennung von dna-molekülen
ATE408705T1 (de) Verfahren und vorrichtung zum aufkonzentrieren und auffinden von mikrobiologischen arten
DK0750637T3 (da) CAI resistens, som koder for et protein, samt anvendelse deraf
ATE452984T1 (de) Verfahren und zusammensetzungen zur extraktion von proteinen aus zellen
DE69928028D1 (de) Verfahren zum Züchten von Silurus Glanis in einer wässrigen Umgebung und Vorrichtung zur Durchführung dieses Verfahrens
DE50110172D1 (de) Verfahren zur altersbestimmung von individuen
EP1048949A3 (de) Schablone und Verfahren zur deren Verwendung
Danielsen et al. Microscale purification of proteins by line immunoelectrophoresis: application of the technique in protein biogenesis studies
ATE397658T1 (de) Verfahren zur zweidimensionalen konformationsabhängigen trennung nichtzirkulärer nukleinsäuren
ATE132896T1 (de) Methoden zur verwendung von an humanen klasse-ii- histokompatibilitätsantigenen mangelnden zellinien
ATE454470T1 (de) Verfahren zum nachweis und zur quantifizierung von hepatitis-c-virus