DE874984T1 - Teststreifen für einen elektochemischen biosensor - Google Patents

Teststreifen für einen elektochemischen biosensor

Info

Publication number
DE874984T1
DE874984T1 DE0874984T DE96922651T DE874984T1 DE 874984 T1 DE874984 T1 DE 874984T1 DE 0874984 T DE0874984 T DE 0874984T DE 96922651 T DE96922651 T DE 96922651T DE 874984 T1 DE874984 T1 DE 874984T1
Authority
DE
Germany
Prior art keywords
distribution network
electrical insulator
reagent
electrodes
opening
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
DE0874984T
Other languages
English (en)
Inventor
Joseph Bateson
Brian Heald
Brian S. Hill
Scott Hubbard
G. Pritchard
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics Corp
Original Assignee
Roche Diagnostics Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics Corp filed Critical Roche Diagnostics Corp
Publication of DE874984T1 publication Critical patent/DE874984T1/de
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
    • C12Q1/006—Enzyme electrodes involving specific analytes or enzymes for glucose

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Emergency Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Secondary Cells (AREA)
  • Hybrid Cells (AREA)

Claims (10)

·* ♦ 3 «ft *· · · 9« 1 DE/EP 0874984T1 Ansprüche
1. Vorrichtung zum Nachweis oder zur Messung der Konzentration eines Analyten, umfassend:
einen ersten elektrischen Isolator;
ein Elektrodenpaar, bestehend aus Arbeits- und Gegenelektrode von im Wesentlichen der gleichen Größe,
wobei die Elektroden aus den gleichen elektrisch leitfähigen Materialien hergestellt sind und auf dem ersten elektrischen Isolator getragen werden;
einen zweiten elektrischen Isolator, der den ersten
elektrischen Isolator und die Elektroden überlagert und einen Ausschnittsbereich umfasst, der einen kleineren Oberflächenbereich von der Gegenelektrode als von der Arbeitselektrode freilässt;
ein Reagenz zum Nachweis oder zur Messung der Konzentration des Analyten, wobei das Reagenz im Wesentlichen die freigelegten Elektrodenoberflächen in dem Ausschnittsbereich bedeckt; und
ein Verteilernetz, imprägniert mit einem oberflächenaktiven Mittel, welches den Ausschnittsbereich überlagert und an dem zweiten elektrischen Isolator befestigt ist,
worin der Ausschnittsbereich und das Verteilernetz eine
ausreichende Größe aufweisen und das Reagenz in einer ausreichenden Menge vorliegt, um eine minimale
2 OE/EP Ö87498U1
Vollblutprobe von etwa 9 Mikroliter zur Analyse des Analyten aufzunehmen.
2. Vorrichtung nach Anspruch 1, worin das Verteilernetz an das
zweite Substrat durch ein Band, welches einen Klebstoff auf einer Seite und eine Öffnung aufweist, die mindestens einen Teil des Verteilernetzes und des Ausschnittsbereichs freilässt, befestigt ist und worin das Band auch mindestens einen Schlitz nahe der Öffnung umfasst, wodurch mindestens ein Luftloch bereitgestellt wird.
3. Vorrichtung nach Anspruch 1, weiterhin umfassend ein Strommessgerät in elektrischer Verbindung mit der Arbeits- und Gegenelektrode.
4. Vorrichtung nach Anspruch 2, worin das Band Schlitze an gegenüberliegenden Seiten der Öffnung umfasst, wodurch zwei Luftlöcher bereitgestellt werden.
5. Vorrichtung nach Anspruch 2, worin der Ausschnittsbereich 4 mm &khgr; 4,2 mm ist.
6. Vorrichtung nach Anspruch 5, worin das Verteilernetz 6 mm &khgr; 5,8 mm ist.
7. Vorrichtung nach Anspruch 6, worin die Reagenzmenge vor dem Trocknen 4&mgr;&Igr; ist.
8. Vorrichtung nach Anspruch 7, worin das Verteilernetz mit Dioctylnatriumsulfosuccinat imprägniert ist.
3- DE/EP Q 874 98&Lgr; Tl
9. Vorrichtung nach Anspruch 8, worin die Öffnung in dem Band im Wesentlichen den gesamten Ausschnittsbereich freilässt.
10. Vorrichtung nach Anspruch 9, weiterhin umfassend ein
Strommessgerät in elektrischer Verbindung mit der Arbeits- und Gegenelektrode.
DE0874984T 1995-06-30 1996-06-28 Teststreifen für einen elektochemischen biosensor Pending DE874984T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/496,939 US5762770A (en) 1994-02-21 1995-06-30 Electrochemical biosensor test strip
PCT/US1996/011240 WO1997002487A1 (en) 1995-06-30 1996-06-28 Electrochemical biosensor test strip

Publications (1)

Publication Number Publication Date
DE874984T1 true DE874984T1 (de) 2000-10-05

Family

ID=23974813

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69617464T Expired - Lifetime DE69617464T2 (de) 1995-06-30 1996-06-28 Teststreifen für einen elektochemischen biosensor
DE0874984T Pending DE874984T1 (de) 1995-06-30 1996-06-28 Teststreifen für einen elektochemischen biosensor

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE69617464T Expired - Lifetime DE69617464T2 (de) 1995-06-30 1996-06-28 Teststreifen für einen elektochemischen biosensor

Country Status (9)

Country Link
US (1) US5762770A (de)
EP (1) EP0874984B1 (de)
JP (1) JP3819936B2 (de)
KR (1) KR100344740B1 (de)
AU (1) AU712527B2 (de)
CA (1) CA2224308C (de)
DE (2) DE69617464T2 (de)
ES (1) ES2154250T3 (de)
WO (1) WO1997002487A1 (de)

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