DE69931053D1 - Verfahren zur reinigung von menschlichen, dem papillomavirus ähnlichen partikeln. - Google Patents

Verfahren zur reinigung von menschlichen, dem papillomavirus ähnlichen partikeln.

Info

Publication number
DE69931053D1
DE69931053D1 DE69931053T DE69931053T DE69931053D1 DE 69931053 D1 DE69931053 D1 DE 69931053D1 DE 69931053 T DE69931053 T DE 69931053T DE 69931053 T DE69931053 T DE 69931053T DE 69931053 D1 DE69931053 D1 DE 69931053D1
Authority
DE
Germany
Prior art keywords
papilloma virus
particles similar
vlps
cleaning human
human particles
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69931053T
Other languages
English (en)
Other versions
DE69931053T2 (de
Inventor
C Cook
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck Sharp and Dohme LLC
Original Assignee
Merck and Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck and Co Inc filed Critical Merck and Co Inc
Application granted granted Critical
Publication of DE69931053D1 publication Critical patent/DE69931053D1/de
Publication of DE69931053T2 publication Critical patent/DE69931053T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C12N7/02—Recovery or purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • A61K2039/5258—Virus-like particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/20011—Papillomaviridae
    • C12N2710/20023—Virus like particles [VLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/20011—Papillomaviridae
    • C12N2710/20034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/20011—Papillomaviridae
    • C12N2710/20051—Methods of production or purification of viral material

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biochemistry (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Veterinary Medicine (AREA)
  • Mycology (AREA)
  • Epidemiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
DE1999631053 1998-08-14 1999-08-10 Verfahren zur reinigung von menschlichen, dem papillomavirus ähnlichen partikeln. Expired - Lifetime DE69931053T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US9656898P 1998-08-14 1998-08-14
US96568P 1998-08-14
PCT/US1999/017930 WO2000009671A1 (en) 1998-08-14 1999-08-10 Process for purifying human papillomavirus virus-like particles

Publications (2)

Publication Number Publication Date
DE69931053D1 true DE69931053D1 (de) 2006-06-01
DE69931053T2 DE69931053T2 (de) 2006-11-23

Family

ID=22257989

Family Applications (1)

Application Number Title Priority Date Filing Date
DE1999631053 Expired - Lifetime DE69931053T2 (de) 1998-08-14 1999-08-10 Verfahren zur reinigung von menschlichen, dem papillomavirus ähnlichen partikeln.

Country Status (24)

Country Link
US (1) US6602697B1 (de)
EP (1) EP1105466B1 (de)
JP (1) JP4440471B2 (de)
KR (1) KR20010072470A (de)
CN (1) CN1354787A (de)
AT (1) ATE324436T1 (de)
AU (1) AU760633B2 (de)
BR (1) BR9913026A (de)
CA (1) CA2339034C (de)
CY (1) CY1105084T1 (de)
CZ (1) CZ2001570A3 (de)
DE (1) DE69931053T2 (de)
DK (1) DK1105466T3 (de)
EA (1) EA200100236A1 (de)
ES (1) ES2262333T3 (de)
HR (1) HRP20010113A2 (de)
HU (1) HUP0103091A3 (de)
IL (1) IL141130A0 (de)
NZ (1) NZ509610A (de)
PL (1) PL347472A1 (de)
PT (1) PT1105466E (de)
SI (1) SI1105466T1 (de)
SK (1) SK2232001A3 (de)
WO (1) WO2000009671A1 (de)

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AU750587B2 (en) * 1998-10-16 2002-07-25 Smithkline Beecham Biologicals (Sa) Adjuvant systems and vaccines
JP2000262280A (ja) * 1999-03-18 2000-09-26 Asahi Optical Co Ltd ウィルスまたはウィルス性抗原の精製方法およびワクチンの製造方法
US6436402B1 (en) * 1999-10-15 2002-08-20 Merck & Co., Inc. Process for making human papillomavirus virus-like particles with improved properties
GB0206360D0 (en) 2002-03-18 2002-05-01 Glaxosmithkline Biolog Sa Viral antigens
MY139500A (en) * 2003-11-12 2009-10-30 Merck Sharp & Dohme Optimized expression of hpv 58 l1 in yeast
WO2005077528A1 (en) * 2004-02-05 2005-08-25 Millipore Corporation Porous adsorptive or chromatographic media
ATE466022T1 (de) * 2004-03-24 2010-05-15 Merck Sharp & Dohme Optimierte expression von hpv-52-l1 in hefe
ATE474596T1 (de) 2004-09-10 2010-08-15 Asahi Glass Co Ltd Impfstoff gegen humanes papillomavirus zur oralen verabreichung
EP1736538A1 (de) * 2005-06-21 2006-12-27 Cytos Biotechnology AG Prozess für die preparative Reinigung von Virus-ähnlichen Partikeln (VLPs)
JP2010502425A (ja) * 2006-08-30 2010-01-28 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション 膜を評価するシステム及び方法並びに膜濾過装置
DK2066354T3 (da) 2006-09-29 2013-05-27 Ligocyte Pharmaceuticals Inc Norovirus vaccine formuleringer
ATE496935T1 (de) * 2007-02-22 2011-02-15 Baxter Int Verfahren zur aufreinigung hydrophober proteine
SG179488A1 (en) 2007-03-14 2012-04-27 Ligocyte Pharmaceuticals Inc Virus like particle purification
BRPI0811016B1 (pt) 2007-04-29 2021-09-21 Xiamen Innovax Biotech Co., Ltd. Proteína li truncada do papiloma vírus humano tipo 16
DK2147926T3 (en) 2007-04-29 2016-11-28 Beijing Wantai Biological Pharmacy Entpr Co Ltd Truncated human papillomavirus type 18 L1 protein
CN101343315B (zh) * 2007-05-29 2012-06-13 厦门大学 截短的人乳头瘤病毒6型l1蛋白
US9433922B2 (en) * 2007-08-14 2016-09-06 Emd Millipore Corporation Media for membrane ion exchange chromatography based on polymeric primary amines, sorption device containing that media, and chromatography scheme and purification method using the same
JP2010539192A (ja) 2007-09-18 2010-12-16 リゴサイト ファーマスーティカルズ，インコーポレイテッド ノロウイルスに対して防御免疫応答を付与する方法
US20100266636A1 (en) * 2007-09-18 2010-10-21 Ligocyte Pharmaceuticals, Inc. Method of conferring a protective immune response to norovirus
EP2011573B1 (de) * 2007-11-05 2010-07-21 Agilent Technologies, Inc. Einfrierung eines mikrofluidischen Chips
US20090130738A1 (en) * 2007-11-19 2009-05-21 Mikhail Kozlov Media for membrane ion exchange chromatography
CN104911154A (zh) 2008-08-08 2015-09-16 武田疫苗股份有限公司 交叉反应性增强的包含复合衣壳氨基酸序列的病毒样颗粒
CA2787009C (en) 2010-01-15 2018-04-03 Bio-Rad Laboratories, Inc. Surface neutralization of apatite
CN102985536B (zh) 2010-04-14 2017-12-05 Emd密理博公司 生产高效价、高纯度的病毒母液的方法及使用其的方法
US8895707B2 (en) 2010-08-18 2014-11-25 Bio-Rad Laboratories, Inc. Elution of proteins from hydroxyapatite resins without resin deterioration
US9592540B2 (en) 2011-02-02 2017-03-14 Bio-Rad Laboratories, Inc. Apatite surface neutralization with alkali solutions
JP6208659B2 (ja) 2011-07-11 2017-10-04 タケダ ワクチン，インコーポレイテッド 非経口ノロウイルスワクチン製剤
WO2013080187A1 (en) 2011-12-01 2013-06-06 University Of Cape Town Hpv chimaeric particle
US9815695B2 (en) 2012-05-30 2017-11-14 Bio-Rad Laboratories, Inc. In situ restoration of apatite-based chromatography resins
JOP20130186B1 (ar) 2012-06-22 2021-08-17 Takeda Vaccines Montana Inc تنقية الجزيئات الشبيهة بالفيروسات
KR101559622B1 (ko) * 2012-07-30 2015-10-13 중앙대학교 산학협력단 인유두종바이러스 바이러스 유사입자의 고효율 정제방법
JP6479305B2 (ja) * 2013-07-12 2019-03-06 株式会社Ｕｍｎファーマ ウイルス様粒子の精製方法
CN105392569B (zh) 2014-06-23 2019-08-20 生物辐射实验室股份有限公司 磷灰石预处理
EP3157551B1 (de) 2014-06-23 2023-02-15 Bio-Rad Laboratories, Inc. In-situ-restaurierung von apatit
CN105907729B (zh) * 2015-05-05 2019-08-16 广东东阳光药业有限公司 一种制备人乳头瘤病毒l1蛋白类病毒样颗粒的方法
CN108524929B (zh) * 2017-03-06 2019-05-07 广州瑞贝斯药业有限公司 一种狂犬病疫苗的生产方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6078999A (ja) 1983-10-05 1985-05-04 Chemo Sero Therapeut Res Inst ＨＢｓ抗原の精製方法
SE9000650L (sv) * 1989-02-28 1990-08-29 Asahi Optical Co Ltd Separation av celler eller virus
PL183781B1 (pl) 1994-05-16 2002-07-31 Merck & Co Inc Sposób wytwarzania szczepionki przeciwko ludzkiemu wirusowi brodawczaków
EP0782615A4 (de) * 1994-09-22 2001-08-22 Merck & Co Inc Kodierende dns von humanen papillomavirus typ 6a
AR004464A1 (es) * 1994-11-14 1998-12-16 Merck Sharp & Dohme Un metodo para producir una proteina de capside de papilomavirus
IL117459A (en) * 1995-03-22 2005-11-20 Merck & Co Inc Dna encoding human papillomavirus type 18
IL117591A0 (en) * 1995-03-30 1996-07-23 Merck & Co Inc Synthetic DNA encoding human papillomavirus type 11 L1 protein
US6143548A (en) 1995-08-30 2000-11-07 Genzyme Corporation Chromatographic purification of adeno-associated virus (AAV)

Also Published As

Publication number Publication date
DE69931053T2 (de) 2006-11-23
WO2000009671A1 (en) 2000-02-24
NZ509610A (en) 2002-10-25
JP4440471B2 (ja) 2010-03-24
EP1105466A1 (de) 2001-06-13
ES2262333T3 (es) 2006-11-16
EP1105466B1 (de) 2006-04-26
AU5469899A (en) 2000-03-06
SK2232001A3 (en) 2001-08-06
US6602697B1 (en) 2003-08-05
BR9913026A (pt) 2001-09-25
HUP0103091A2 (hu) 2001-12-28
PT1105466E (pt) 2006-07-31
CN1354787A (zh) 2002-06-19
DK1105466T3 (da) 2006-08-14
SI1105466T1 (sl) 2006-08-31
IL141130A0 (en) 2002-02-10
JP2003520188A (ja) 2003-07-02
CA2339034A1 (en) 2000-02-24
AU760633B2 (en) 2003-05-22
CA2339034C (en) 2010-10-12
HRP20010113A2 (en) 2002-02-28
ATE324436T1 (de) 2006-05-15
CY1105084T1 (el) 2009-11-04
PL347472A1 (en) 2002-04-08
KR20010072470A (ko) 2001-07-31
HUP0103091A3 (en) 2002-03-28
CZ2001570A3 (cs) 2001-09-12
EA200100236A1 (ru) 2001-08-27
EP1105466A4 (de) 2002-05-15

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