CY1105084T1 - Διepγασια για καθαρισμο σωματιδιων που ομοιαζουν με τα σωματιδια ιου θηλωματων - Google Patents

Διepγασια για καθαρισμο σωματιδιων που ομοιαζουν με τα σωματιδια ιου θηλωματων

Info

Publication number
CY1105084T1
CY1105084T1 CY20061100963T CY061100963T CY1105084T1 CY 1105084 T1 CY1105084 T1 CY 1105084T1 CY 20061100963 T CY20061100963 T CY 20061100963T CY 061100963 T CY061100963 T CY 061100963T CY 1105084 T1 CY1105084 T1 CY 1105084T1
Authority
CY
Cyprus
Prior art keywords
particles
vlps
pammila
diagrams
purification
Prior art date
Application number
CY20061100963T
Other languages
English (en)
Inventor
James C. Cook, Iii
Original Assignee
Merck & Co., Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck & Co., Inc. filed Critical Merck & Co., Inc.
Publication of CY1105084T1 publication Critical patent/CY1105084T1/el

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C12N7/02—Recovery or purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • A61K2039/5258—Virus-like particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/20011—Papillomaviridae
    • C12N2710/20023—Virus like particles [VLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/20011—Papillomaviridae
    • C12N2710/20034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/20011—Papillomaviridae
    • C12N2710/20051—Methods of production or purification of viral material

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Engineering & Computer Science (AREA)
  • Veterinary Medicine (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Biochemistry (AREA)
  • Epidemiology (AREA)
  • Mycology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

Μία διεργασία για καθαρισμό σωματιδίων (VLPs) που ομοιάζουν με τα σωματίδια ιού θηλωμάτων περιλαμβάνει το στάδιο περάσματος μερικώς καθαρισμένου διαλύματος που περιέχει VLP, διαμέσου χρωματογραφικής στήλης υδροξυαπατίτη. Τα VLPs στην συνέχεια εκλούονται με ένα ρυθμιστικό διάλυμα που περιέχει φωσφορικά ανιόντα. Τα πλεονεκτήματα αυτής της μεθόδου περιλαμβάνουν την ανάκτηση υψηλής απόδοσης ακέραιων VLPs.
CY20061100963T 1998-08-14 2006-07-11 Διepγασια για καθαρισμο σωματιδιων που ομοιαζουν με τα σωματιδια ιου θηλωματων CY1105084T1 (el)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9656898P 1998-08-14 1998-08-14
PCT/US1999/017930 WO2000009671A1 (en) 1998-08-14 1999-08-10 Process for purifying human papillomavirus virus-like particles

Publications (1)

Publication Number Publication Date
CY1105084T1 true CY1105084T1 (el) 2009-11-04

Family

ID=22257989

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20061100963T CY1105084T1 (el) 1998-08-14 2006-07-11 Διepγασια για καθαρισμο σωματιδιων που ομοιαζουν με τα σωματιδια ιου θηλωματων

Country Status (24)

Country Link
US (1) US6602697B1 (el)
EP (1) EP1105466B1 (el)
JP (1) JP4440471B2 (el)
KR (1) KR20010072470A (el)
CN (1) CN1354787A (el)
AT (1) ATE324436T1 (el)
AU (1) AU760633B2 (el)
BR (1) BR9913026A (el)
CA (1) CA2339034C (el)
CY (1) CY1105084T1 (el)
CZ (1) CZ2001570A3 (el)
DE (1) DE69931053T2 (el)
DK (1) DK1105466T3 (el)
EA (1) EA200100236A1 (el)
ES (1) ES2262333T3 (el)
HR (1) HRP20010113A2 (el)
HU (1) HUP0103091A3 (el)
IL (1) IL141130A0 (el)
NZ (1) NZ509610A (el)
PL (1) PL347472A1 (el)
PT (1) PT1105466E (el)
SI (1) SI1105466T1 (el)
SK (1) SK2232001A3 (el)
WO (1) WO2000009671A1 (el)

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JP2000262280A (ja) * 1999-03-18 2000-09-26 Asahi Optical Co Ltd ウィルスまたはウィルス性抗原の精製方法およびワクチンの製造方法
US6436402B1 (en) * 1999-10-15 2002-08-20 Merck & Co., Inc. Process for making human papillomavirus virus-like particles with improved properties
GB0206360D0 (en) 2002-03-18 2002-05-01 Glaxosmithkline Biolog Sa Viral antigens
MY139500A (en) * 2003-11-12 2009-10-30 Merck Sharp & Dohme Optimized expression of hpv 58 l1 in yeast
SG150501A1 (en) * 2004-02-05 2009-03-30 Millipore Corp Porous adsorptive or chromatographic media
CA2560487C (en) * 2004-03-24 2013-01-29 Merck & Co., Inc. Optimized expression of hpv 52 l1 in yeast
CA2579882C (en) 2004-09-10 2012-07-17 Asahi Glass Company, Limited Edible vaccine
EP1736538A1 (en) * 2005-06-21 2006-12-27 Cytos Biotechnology AG Process for the preparative purification of virus-like-particles (VLPs)
US20100288029A1 (en) * 2006-08-30 2010-11-18 Ge Healthcar Bio-Sciences Corp. System and method for characterizing membranes and membrane filtration devices
AU2008302276B2 (en) 2006-09-29 2013-10-10 Takeda Vaccines, Inc. Method of conferring a protective immune response to norovirus
KR101515489B1 (ko) 2006-09-29 2015-04-30 다케다 백신즈 인코포레이티드 노로바이러스 백신 제제
AU2008217189B2 (en) * 2007-02-22 2013-06-13 Baxalta GmbH Method of purification of hydrophobic proteins
JP5284290B2 (ja) 2007-03-14 2013-09-11 リゴサイト ファーマスーティカルズ，インコーポレイテッド ウイルス様粒子の精製
WO2008134935A1 (fr) 2007-04-29 2008-11-13 Beijing Wantai Biological Pharmacy Enterprise Co., Ltd. Protéines 18 l1 de type papillomavirus humain tronqué
CN101293918B (zh) 2007-04-29 2013-03-27 北京万泰生物药业股份有限公司 截短的人乳头瘤病毒16型l1蛋白
WO2008145021A1 (en) * 2007-05-29 2008-12-04 Xiamen University A truncated l1 protein of human papillomavirus 6
US9433922B2 (en) * 2007-08-14 2016-09-06 Emd Millipore Corporation Media for membrane ion exchange chromatography based on polymeric primary amines, sorption device containing that media, and chromatography scheme and purification method using the same
US20100266636A1 (en) * 2007-09-18 2010-10-21 Ligocyte Pharmaceuticals, Inc. Method of conferring a protective immune response to norovirus
DE602007007927D1 (de) * 2007-11-05 2010-09-02 Agilent Technologies Inc Einfrierung eines mikrofluidischen Chips
US20090130738A1 (en) * 2007-11-19 2009-05-21 Mikhail Kozlov Media for membrane ion exchange chromatography
SG2013057732A (en) * 2008-08-08 2015-02-27 Ligocyte Pharmaceuticals Inc Virus-like particles comprising composite capsid amino acid sequences for enhanced cross reactivity
US8951807B2 (en) * 2010-01-15 2015-02-10 Bio-Rad Laboratories, Inc. Surface neutralization of apatite
CN102985536B (zh) 2010-04-14 2017-12-05 Emd密理博公司 生产高效价、高纯度的病毒母液的方法及使用其的方法
US8895707B2 (en) 2010-08-18 2014-11-25 Bio-Rad Laboratories, Inc. Elution of proteins from hydroxyapatite resins without resin deterioration
JP5881744B2 (ja) 2011-02-02 2016-03-09 バイオ−ラッド ラボラトリーズ インコーポレーティッド アルカリ溶液でのアパタイトの表面中和方法
EA029470B1 (ru) 2011-07-11 2018-03-30 Такеда Вэксинс, Инк. Способ стимулирования формирования защитного иммунитета против норовируса
CA2850293C (en) * 2011-12-01 2019-10-08 University Of Cape Town Hpv chimaeric particle
WO2013180933A1 (en) 2012-05-30 2013-12-05 Bio-Rad Laboratories, Inc. In situ restoration of apatite-based chromatography resins
JOP20130186B1 (ar) 2012-06-22 2021-08-17 Takeda Vaccines Montana Inc تنقية الجزيئات الشبيهة بالفيروسات
KR101559622B1 (ko) * 2012-07-30 2015-10-13 중앙대학교 산학협력단 인유두종바이러스 바이러스 유사입자의 고효율 정제방법
JP6479305B2 (ja) * 2013-07-12 2019-03-06 株式会社Ｕｍｎファーマ ウイルス様粒子の精製方法
US10099157B2 (en) 2014-06-23 2018-10-16 Bio-Rad Laboratories, Inc. Apatite in-situ restoration
WO2015200282A1 (en) 2014-06-23 2015-12-30 Bio-Rad Laboratories, Inc. Apatite pretreatment
CN105907729B (zh) * 2015-05-05 2019-08-16 广东东阳光药业有限公司 一种制备人乳头瘤病毒l1蛋白类病毒样颗粒的方法
CN108524929B (zh) * 2017-03-06 2019-05-07 广州瑞贝斯药业有限公司 一种狂犬病疫苗的生产方法

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Also Published As

Publication number Publication date
WO2000009671A1 (en) 2000-02-24
US6602697B1 (en) 2003-08-05
AU760633B2 (en) 2003-05-22
HUP0103091A2 (hu) 2001-12-28
JP4440471B2 (ja) 2010-03-24
CA2339034C (en) 2010-10-12
SK2232001A3 (en) 2001-08-06
SI1105466T1 (sl) 2006-08-31
EP1105466B1 (en) 2006-04-26
HRP20010113A2 (en) 2002-02-28
IL141130A0 (en) 2002-02-10
HUP0103091A3 (en) 2002-03-28
CZ2001570A3 (cs) 2001-09-12
ES2262333T3 (es) 2006-11-16
EP1105466A4 (en) 2002-05-15
PT1105466E (pt) 2006-07-31
EP1105466A1 (en) 2001-06-13
EA200100236A1 (ru) 2001-08-27
AU5469899A (en) 2000-03-06
DE69931053T2 (de) 2006-11-23
PL347472A1 (en) 2002-04-08
BR9913026A (pt) 2001-09-25
ATE324436T1 (de) 2006-05-15
NZ509610A (en) 2002-10-25
DE69931053D1 (de) 2006-06-01
KR20010072470A (ko) 2001-07-31
DK1105466T3 (da) 2006-08-14
CN1354787A (zh) 2002-06-19
JP2003520188A (ja) 2003-07-02
CA2339034A1 (en) 2000-02-24

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