BRPI0210106B8 - processo para a purificação de caseinase, colagenases dos tipos i e ii e clostripaína produzidas em um meio pela fermentação de clostridium histolyticum - Google Patents

processo para a purificação de caseinase, colagenases dos tipos i e ii e clostripaína produzidas em um meio pela fermentação de clostridium histolyticum

Info

Publication number
BRPI0210106B8
BRPI0210106B8 BRPI0210106A BRPI0210106B8 BR PI0210106 B8 BRPI0210106 B8 BR PI0210106B8 BR PI0210106 A BRPI0210106 A BR PI0210106A BR PI0210106 B8 BRPI0210106 B8 BR PI0210106B8
Authority
BR
Brazil
Prior art keywords
purification
fermentation
clostripaine
caseinase
collagenases
Prior art date
Application number
Other languages
English (en)
Inventor
Kurfuerst Manfred
Schmidbauer Stefan
Original Assignee
Nordmark Arzneimittel Gmbh & Co Kg
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nordmark Arzneimittel Gmbh & Co Kg filed Critical Nordmark Arzneimittel Gmbh & Co Kg
Priority claimed from PCT/EP2002/006021 external-priority patent/WO2003004628A2/de
Publication of BR0210106A publication Critical patent/BR0210106A/pt
Publication of BRPI0210106B1 publication Critical patent/BRPI0210106B1/pt
Publication of BRPI0210106B8 publication Critical patent/BRPI0210106B8/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/22—Cysteine endopeptidases (3.4.22)
    • C12Y304/22008—Clostripain (3.4.22.8)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Public Health (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Animal Behavior & Ethology (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

"processo para a purificação de uma enzima e enzima purificada, preparada de acordo com este bem como aplicação da enzima". a invenção refere-se a um processo para a separação de pelo menos uma enzima contida em um sobrenadante de fermentação de clostridium histolyticum. é previsto, que as enzimas do sobrenadante de fermentação são separadas por um processo de cromatografia de várias etapas sob emprego exclusivo de materiais de cromatografia à base de estireno/divinilbenzeno e/ou à base de hidroxilapatita, especialmente cerâmico.
BR0210106-8 2001-07-02 2002-06-01 processo para a purificação de caseinase, colagenases dos tipos i e ii e clostripaína produzidas em um meio pela fermentação de clostridium histolyticum BRPI0210106B8 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DE10131994.0 2001-07-02
DE10131994 2001-07-02
DE10134347A DE10134347B4 (de) 2001-07-02 2001-07-14 Verfahren zur Aufreinigung eines Enzyms
DE10134347.7 2001-07-14
PCT/EP2002/006021 WO2003004628A2 (de) 2001-07-02 2002-06-01 Verfahren zur aufreinigung eines enzyms und hiernach hergestelltes, aufgereinigtes enzym sowie verwendung des enzyms

Publications (3)

Publication Number Publication Date
BR0210106A BR0210106A (pt) 2004-07-27
BRPI0210106B1 BRPI0210106B1 (pt) 2018-04-03
BRPI0210106B8 true BRPI0210106B8 (pt) 2021-05-25

Family

ID=7690313

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0210106-8 BRPI0210106B8 (pt) 2001-07-02 2002-06-01 processo para a purificação de caseinase, colagenases dos tipos i e ii e clostripaína produzidas em um meio pela fermentação de clostridium histolyticum

Country Status (2)

Country Link
BR (1) BRPI0210106B8 (pt)
DE (2) DE10134347B4 (pt)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3924363A1 (de) 2019-02-14 2021-12-22 Nordmark Pharma GmbH Chromatographische aufreinigung von wenigstens einem enzym, ausgesucht aus der gruppe bestehend aus kollagenase typ i, kollagenase typ ii, neutrale protease und clostripain

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58209980A (ja) * 1982-04-27 1983-12-07 Kyowa Hakko Kogyo Co Ltd 酵素の精製法
US4798886A (en) * 1986-03-24 1989-01-17 Takeda Chemical Industries, Ltd. Mutual separation of proteins

Also Published As

Publication number Publication date
BRPI0210106B1 (pt) 2018-04-03
BR0210106A (pt) 2004-07-27
DE10134347B4 (de) 2006-09-28
DE10134347A1 (de) 2003-01-23
DE50204038D1 (de) 2005-09-29

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B15K Others concerning applications: alteration of classification

Free format text: A CLASSIFICACAO ANTERIOR ERA: C12N 9/00

Ipc: C12P 1/04 (2006.01), C12P 21/00 (2006.01), C12N 9/

B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]
B07E Notification of approval relating to section 229 industrial property law [chapter 7.5 patent gazette]

Free format text: NOTIFICACAO DE ANUENCIA RELACIONADA COM O ART 229 DA LPI

B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]
B12B Appeal against refusal [chapter 12.2 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]
B16C Correction of notification of the grant [chapter 16.3 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 01/06/2002 OBSERVADAS AS CONDICOES LEGAIS. PATENTE CONCEDIDA CONFORME ADI 5.529/DF

B25D Requested change of name of applicant approved
B25A Requested transfer of rights approved
B21F Lapse acc. art. 78, item iv - on non-payment of the annual fees in time

Free format text: REFERENTE A 21A ANUIDADE.

B24J Lapse because of non-payment of annual fees (definitively: art 78 iv lpi, resolution 113/2013 art. 12)

Free format text: EM VIRTUDE DA EXTINCAO PUBLICADA NA RPI 2725 DE 28-03-2023 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDA A EXTINCAO DA PATENTE E SEUS CERTIFICADOS, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.