DE10134347B4 - Verfahren zur Aufreinigung eines Enzyms - Google Patents

Verfahren zur Aufreinigung eines Enzyms Download PDF

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Publication number
DE10134347B4
DE10134347B4 DE10134347A DE10134347A DE10134347B4 DE 10134347 B4 DE10134347 B4 DE 10134347B4 DE 10134347 A DE10134347 A DE 10134347A DE 10134347 A DE10134347 A DE 10134347A DE 10134347 B4 DE10134347 B4 DE 10134347B4
Authority
DE
Germany
Prior art keywords
type
collagenase
chromatographic
elution
chromatography
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE10134347A
Other languages
German (de)
English (en)
Other versions
DE10134347A1 (de
Inventor
Manfred Dr. Kurfürst
Stefan Dr. Schmidbauer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nordmark Arzneimittel GmbH and Co KG
Original Assignee
Nordmark Arzneimittel GmbH and Co KG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to DE10134347A priority Critical patent/DE10134347B4/de
Application filed by Nordmark Arzneimittel GmbH and Co KG filed Critical Nordmark Arzneimittel GmbH and Co KG
Priority to DE50204038T priority patent/DE50204038D1/de
Priority to EP02782420A priority patent/EP1402008B1/de
Priority to AT02782420T priority patent/ATE302844T1/de
Priority to BRPI0210106-8 priority patent/BRPI0210106B8/pt
Priority to PCT/EP2002/006021 priority patent/WO2003004628A2/de
Priority to AU2002354696A priority patent/AU2002354696A1/en
Priority to JP2003510787A priority patent/JP4266817B2/ja
Priority to CA2447818A priority patent/CA2447818C/en
Priority to CNB028131347A priority patent/CN1308442C/zh
Priority to US10/478,545 priority patent/US7083964B2/en
Publication of DE10134347A1 publication Critical patent/DE10134347A1/de
Application granted granted Critical
Publication of DE10134347B4 publication Critical patent/DE10134347B4/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/22—Cysteine endopeptidases (3.4.22)
    • C12Y304/22008—Clostripain (3.4.22.8)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Enzymes And Modification Thereof (AREA)
DE10134347A 2001-07-02 2001-07-14 Verfahren zur Aufreinigung eines Enzyms Expired - Fee Related DE10134347B4 (de)

Priority Applications (11)

Application Number Priority Date Filing Date Title
DE10134347A DE10134347B4 (de) 2001-07-02 2001-07-14 Verfahren zur Aufreinigung eines Enzyms
CA2447818A CA2447818C (en) 2001-07-02 2002-06-01 Method for purifying an enzyme and purified enzyme produced according to this method, as well as use of the enzyme
AT02782420T ATE302844T1 (de) 2001-07-02 2002-06-01 Verfahren zur aufreinigung eines enzyms und hiernach hergestelltes, aufgereinigtes enzym sowie verwendung des enzyms
BRPI0210106-8 BRPI0210106B8 (pt) 2001-07-02 2002-06-01 processo para a purificação de caseinase, colagenases dos tipos i e ii e clostripaína produzidas em um meio pela fermentação de clostridium histolyticum
PCT/EP2002/006021 WO2003004628A2 (de) 2001-07-02 2002-06-01 Verfahren zur aufreinigung eines enzyms und hiernach hergestelltes, aufgereinigtes enzym sowie verwendung des enzyms
AU2002354696A AU2002354696A1 (en) 2001-07-02 2002-06-01 Method for purifying an enzyme, a purified enzyme produced thereby, and use of this enzyme
DE50204038T DE50204038D1 (de) 2001-07-02 2002-06-01 Verfahren zur aufreinigung eines enzyms und hiernach hergestelltes, aufgereinigtes enzym sowie verwendung des enzyms
EP02782420A EP1402008B1 (de) 2001-07-02 2002-06-01 Verfahren zur aufreinigung eines enzyms und hiernach hergestelltes, aufgereinigtes enzym sowie verwendung des enzyms
CNB028131347A CN1308442C (zh) 2001-07-02 2002-06-01 纯化酶的方法和按此产生出的经纯化的酶以及该酶的用途
US10/478,545 US7083964B2 (en) 2001-07-02 2002-06-01 Method for purifying enzymes from clostridium histolyticum using multi-stage chromatography
JP2003510787A JP4266817B2 (ja) 2001-07-02 2002-06-01 酵素の精製方法およびその方法で製造された精製済み酵素並びに該酵素の用途

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10131994 2001-07-02
DE10131994.0 2001-07-02
DE10134347A DE10134347B4 (de) 2001-07-02 2001-07-14 Verfahren zur Aufreinigung eines Enzyms

Publications (2)

Publication Number Publication Date
DE10134347A1 DE10134347A1 (de) 2003-01-23
DE10134347B4 true DE10134347B4 (de) 2006-09-28

Family

ID=7690313

Family Applications (2)

Application Number Title Priority Date Filing Date
DE10134347A Expired - Fee Related DE10134347B4 (de) 2001-07-02 2001-07-14 Verfahren zur Aufreinigung eines Enzyms
DE50204038T Expired - Lifetime DE50204038D1 (de) 2001-07-02 2002-06-01 Verfahren zur aufreinigung eines enzyms und hiernach hergestelltes, aufgereinigtes enzym sowie verwendung des enzyms

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE50204038T Expired - Lifetime DE50204038D1 (de) 2001-07-02 2002-06-01 Verfahren zur aufreinigung eines enzyms und hiernach hergestelltes, aufgereinigtes enzym sowie verwendung des enzyms

Country Status (2)

Country Link
BR (1) BRPI0210106B8 (pt)
DE (2) DE10134347B4 (pt)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20220135617A1 (en) 2019-02-14 2022-05-05 Nordmark Pharma Gmbh Chromatographic purification of at least one enzyme selected from a group including collagenase type i, collagenase type ii, neutral protease and clostripain

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4560661A (en) * 1982-04-27 1985-12-24 Kyowa Hakko Kogyo Co., Ltd. Process for purifying enzymes
US4798886A (en) * 1986-03-24 1989-01-17 Takeda Chemical Industries, Ltd. Mutual separation of proteins

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4560661A (en) * 1982-04-27 1985-12-24 Kyowa Hakko Kogyo Co., Ltd. Process for purifying enzymes
US4798886A (en) * 1986-03-24 1989-01-17 Takeda Chemical Industries, Ltd. Mutual separation of proteins

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
KLOCK G. u.a. Zusammensetzung aud Entrez Pubmed: Fractions from commercial collagenase prepara- tions: use in encymic isolation of the islets of Langerhans from porcine pancreas (1996) Cell Transplant. Vol. 5 (5) Seite 543-551 *

Also Published As

Publication number Publication date
BRPI0210106B8 (pt) 2021-05-25
BR0210106A (pt) 2004-07-27
DE10134347A1 (de) 2003-01-23
DE50204038D1 (de) 2005-09-29
BRPI0210106B1 (pt) 2018-04-03

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee