DE69818458T3 - Ribosom-komplexe als selektionspartikel für die in vitro anzeige und evolution von proteinen - Google Patents
Ribosom-komplexe als selektionspartikel für die in vitro anzeige und evolution von proteinen Download PDFInfo
- Publication number
- DE69818458T3 DE69818458T3 DE69818458T DE69818458T DE69818458T3 DE 69818458 T3 DE69818458 T3 DE 69818458T3 DE 69818458 T DE69818458 T DE 69818458T DE 69818458 T DE69818458 T DE 69818458T DE 69818458 T3 DE69818458 T3 DE 69818458T3
- Authority
- DE
- Germany
- Prior art keywords
- mrna
- protein
- peptide
- dna
- pcr
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1041—Ribosome/Polysome display, e.g. SPERT, ARM
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB9710829.4A GB9710829D0 (en) | 1997-05-28 | 1997-05-28 | Selection particles for in vitro display and evolution of antibody combining sites |
| GB9710829 | 1997-05-28 | ||
| GBGB9724850.4A GB9724850D0 (en) | 1997-11-26 | 1997-11-26 | Selection particles for in vitro display and evolution of antibody combining sites |
| GB9724850 | 1997-11-26 | ||
| GBGB9804195.7A GB9804195D0 (en) | 1998-02-28 | 1998-02-28 | Selection particles for in vitro display and evolution of antibody combining sites |
| GB9804195 | 1998-02-28 | ||
| PCT/GB1998/001564 WO1998054312A1 (en) | 1997-05-28 | 1998-05-28 | Ribosome complexes as selection particles for in vitro display and evolution of proteins |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| DE69818458D1 DE69818458D1 (de) | 2003-10-30 |
| DE69818458T2 DE69818458T2 (de) | 2004-07-08 |
| DE69818458T3 true DE69818458T3 (de) | 2009-12-17 |
Family
ID=27268863
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69818458T Expired - Lifetime DE69818458T3 (de) | 1997-05-28 | 1998-05-28 | Ribosom-komplexe als selektionspartikel für die in vitro anzeige und evolution von proteinen |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US6620587B1 (https=) |
| EP (1) | EP0985032B2 (https=) |
| JP (1) | JP4060892B2 (https=) |
| AT (1) | ATE250668T1 (https=) |
| CA (1) | CA2291721C (https=) |
| DE (1) | DE69818458T3 (https=) |
| DK (1) | DK0985032T4 (https=) |
| ES (1) | ES2209145T5 (https=) |
| PT (1) | PT985032E (https=) |
| WO (1) | WO1998054312A1 (https=) |
Families Citing this family (75)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU4854999A (en) * | 1998-07-02 | 2000-01-24 | Invitro Diagnostics, Inc. | Methods, compositions and apparatus for making nucleic acid molecules having a selected affinity to a target molecule |
| DE19932688B4 (de) | 1999-07-13 | 2009-10-08 | Scil Proteins Gmbh | Design von Beta-Faltblatt-Proteinen des gamma-II-kristallins antikörperähnlichen |
| GB9917027D0 (en) * | 1999-07-20 | 1999-09-22 | Affibody Technology Sweeden Ab | In vitro selection and optional identification of polypeptides using solid support carriers |
| IL150291A0 (en) | 2000-01-11 | 2002-12-01 | Maxygen Inc | Integrated systems and methods for diversity generation and screening |
| EP1652919A1 (en) * | 2000-03-31 | 2006-05-03 | Cambridge Antibody Technology LTD | Improvements to ribosome display |
| ES2253358T3 (es) * | 2000-03-31 | 2006-06-01 | Cambridge Antibody Technology Limited | Mejoras en la visualizacion del ribosoma. |
| US7129326B2 (en) | 2000-04-14 | 2006-10-31 | Genencor International, Inc. | Methods for selective targeting |
| DK1360500T3 (da) | 2000-04-14 | 2010-05-25 | Genencor Int | Fremgangsmåde til selektiv målretning |
| CA2440649A1 (en) | 2001-03-07 | 2002-09-19 | Children's Medical Center Corporation | Method to screen peptide display libraries using minicell display |
| US20060234231A1 (en) * | 2001-06-20 | 2006-10-19 | Nuevolution A/S | Microarrays displaying encoded molecules |
| EP1401850A1 (en) | 2001-06-20 | 2004-03-31 | Nuevolution A/S | Nucleoside derivatives for library preparation |
| DE10145226A1 (de) * | 2001-09-13 | 2003-04-10 | Lifebits Ag | Herstellung von trägergebundenen Molekülen |
| DE10147074A1 (de) * | 2001-09-25 | 2003-05-08 | Beru Ag | Verfahren zum Betreiben einer aus mehreren Heizelementen bestehenden mehrstufigen elektrischen Heizung |
| DE20200926U1 (de) * | 2002-01-23 | 2002-04-18 | Hegenscheidt-MFD GmbH & Co. KG, 41812 Erkelenz | Festwalzgerät einer Festwalzmaschine für Kurbelwellen |
| EP1487978B1 (en) * | 2002-03-15 | 2008-11-19 | Nuevolution A/S | An improved method for synthesising templated molecules |
| US20050221318A1 (en) * | 2002-03-15 | 2005-10-06 | Gouliaev Alex H | Building block forming a c-c bond upon reaction |
| AU2003214033A1 (en) * | 2002-03-15 | 2003-09-29 | Nuevolution A/S | A building block capable of transferring a functional entity |
| AU2003218629A1 (en) * | 2002-03-15 | 2003-09-29 | Nuevolution A/S | A building block forming a c-c or a c-hetero atom bond uponreaction |
| WO2004013070A2 (en) * | 2002-08-01 | 2004-02-12 | Nuevolution A/S | Multi-step synthesis of templated molecules |
| JP4664677B2 (ja) * | 2002-09-06 | 2011-04-06 | イソゲニカ リミテッド | イン・ビトロでのペプチド発現ライブラリー |
| PT2348125T (pt) | 2002-10-30 | 2017-08-29 | Nuevolution As | Método para a síntese de um complexo bifuncional |
| EP2581383A1 (en) | 2002-11-25 | 2013-04-17 | Genencor International, Inc. | Skin or hair binding peptides |
| AU2003291964A1 (en) * | 2002-12-19 | 2004-07-14 | Nuevolution A/S | Quasirandom structure and function guided synthesis methods |
| EP1597394A2 (en) * | 2003-02-21 | 2005-11-23 | Nuevolution A/S | A method for obtaining structural information about an encoded molecule |
| US20070026397A1 (en) * | 2003-02-21 | 2007-02-01 | Nuevolution A/S | Method for producing second-generation library |
| ES2326649T3 (es) * | 2003-03-19 | 2009-10-16 | Crescendo Biologics Ltd. | Recuperacion de dna. |
| DE602004019764D1 (de) * | 2003-03-20 | 2009-04-16 | Nuevolution As | Ligationsvermittelnde codierung von kleinen molekülen |
| DE10324447A1 (de) | 2003-05-28 | 2004-12-30 | Scil Proteins Gmbh | Generierung künstlicher Bindungsproteine auf der Grundlage von Ubiquitin |
| GB0316314D0 (en) * | 2003-07-12 | 2003-08-13 | Univ Belfast | Method |
| EP1670939B1 (en) * | 2003-09-18 | 2009-11-04 | Nuevolution A/S | A method for obtaining structural information concerning an encoded molecule and method for selecting compounds |
| EP1723255B1 (en) * | 2004-02-17 | 2010-12-29 | Nuevolution A/S | Method for enrichment involving elimination by mismatch hybridisation |
| DE602005017370D1 (de) * | 2004-03-22 | 2009-12-10 | Nuevolution As | Ligationscodierung unter verwendung von oligonukleotidbausteinen |
| US20050250112A1 (en) * | 2004-05-07 | 2005-11-10 | Padmabandu Gothami A | Nucleic acids, compositions, methods, and kits for detecting mycoplasma and acholeplasma species |
| US9006393B1 (en) | 2004-08-26 | 2015-04-14 | Patricia L. Clark | Molecular constructs and uses thereof in ribosomal translational events |
| DE102004049479A1 (de) | 2004-10-11 | 2006-04-13 | Scil Proteins Gmbh | Proteinkonjugate zur Verwendung in Therapie, Diagnose und Chromatographie |
| HUE056836T2 (hu) | 2005-12-01 | 2022-03-28 | Nuevolution As | Enzimatikus kódolási eljárások nagy könyvtárak hatékony szintéziséhez |
| GB0605584D0 (en) | 2006-03-20 | 2006-04-26 | Olink Ab | Method for analyte detection using proximity probes |
| US7879974B2 (en) * | 2006-06-29 | 2011-02-01 | The Invention Science Fund I, Llc | Methods for arbitrary peptide synthesis |
| US7879975B2 (en) * | 2006-06-29 | 2011-02-01 | The Invention Science Fund I, Llc | Methods for arbitrary peptide synthesis |
| US7923533B2 (en) * | 2006-06-29 | 2011-04-12 | The Invention Science Fund I, Llc | Methods for arbitrary peptide synthesis |
| US7910695B2 (en) | 2006-06-29 | 2011-03-22 | The Invention Science Fund I, Llc | Methods for arbitrary peptide synthesis |
| US7777002B2 (en) * | 2006-06-29 | 2010-08-17 | The Invention Science Fund I, Llc | Methods for arbitrary peptide synthesis |
| EP1925664A1 (en) | 2006-11-15 | 2008-05-28 | Scil proteins GmbH | Artificial binding proteins based on a modified alpha helical region of ubiquitin |
| US8865179B2 (en) * | 2008-01-26 | 2014-10-21 | Swey-Shen Alexchen | Aptameric IgE peptides in a protein scaffold as an allergy vaccine |
| EP3629022A1 (en) | 2008-07-25 | 2020-04-01 | Richard W. Wagner | Protein screening methods |
| MX2011003381A (es) * | 2008-09-30 | 2011-04-21 | Abbott Lab | Metodo mejorado para despliegue de arn. |
| AU2010332932B2 (en) | 2009-12-14 | 2013-01-17 | Navigo Proteins Gmbh | Modified ubiquitin proteins having a specific binding activity for the extradomain B of fibronectin |
| WO2011111832A1 (ja) | 2010-03-11 | 2011-09-15 | 独立行政法人理化学研究所 | ポリペプチド配列の選択方法、並びに金属酸化物または含珪素化合物結合ペプチドおよびその利用 |
| GB201004292D0 (en) | 2010-03-15 | 2010-04-28 | Olink Ab | Assay for localised detection of analytes |
| WO2011127933A1 (en) | 2010-04-16 | 2011-10-20 | Nuevolution A/S | Bi-functional complexes and methods for making and using such complexes |
| GB201011971D0 (en) | 2010-07-15 | 2010-09-01 | Olink Ab | Methods and product |
| EP2627781B1 (en) | 2010-10-15 | 2017-02-22 | Olink Bioscience AB | Dynamic range methods |
| EP2466310A1 (en) | 2010-12-17 | 2012-06-20 | Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt GmbH | Means and methods for detecting double-strand breaks |
| DE102010056289A1 (de) | 2010-12-24 | 2012-06-28 | Geneart Ag | Verfahren zur Herstellung von Leseraster-korrekten Fragment-Bibliotheken |
| GB201101621D0 (en) | 2011-01-31 | 2011-03-16 | Olink Ab | Method and product |
| JP5948673B2 (ja) | 2011-03-10 | 2016-07-06 | ジーンフロンティア株式会社 | リポ多糖混入量を低減した蛋白質合成用組成物、該組成物を用いた蛋白質製造方法 |
| GB201107863D0 (en) | 2011-05-11 | 2011-06-22 | Olink Ab | Method and product |
| WO2012161227A1 (ja) | 2011-05-23 | 2012-11-29 | 独立行政法人理化学研究所 | 核酸構築物、核酸-蛋白質複合体、及びその利用 |
| JP6146821B2 (ja) | 2011-06-15 | 2017-06-14 | ナフィゴ プロテインズ ゲゼルシャフト ミット ベシュレンクテル ハフツングNavigo Proteins GmbH | 修飾ユビキチンに基づく二量体型結合タンパク質 |
| GB201201547D0 (en) | 2012-01-30 | 2012-03-14 | Olink Ab | Method and product |
| US10421958B2 (en) | 2014-02-05 | 2019-09-24 | Molecular Templates, Inc. | Methods of screening, selecting, and identifying cytotoxic recombinant polypeptides based on an interim diminution of ribotoxicity |
| US20170306039A1 (en) * | 2014-10-22 | 2017-10-26 | Crescendo Biologics Limited | Human vh domain scaffolds |
| JP6738340B2 (ja) | 2015-02-06 | 2020-08-12 | ナフィゴ プロテインズ ゲゼルシャフト ミット ベシュレンクテル ハフツングNavigo Proteins GmbH | 新規なegfr結合タンパク質 |
| AU2016293063B2 (en) | 2015-07-16 | 2018-11-08 | Navigo Proteins Gmbh | Novel immunoglobulin-binding proteins and their use in affinity purification |
| JP2018520675A (ja) | 2015-07-20 | 2018-08-02 | ナフィゴ プロテインズ ゲゼルシャフト ミット ベシュレンクテル ハフツングNavigo Proteins GmbH | ジユビキチン変異タンパク質をベースとした新規結合タンパク質及びその生成方法 |
| KR20180085717A (ko) | 2015-09-24 | 2018-07-27 | 에이비비트로, 엘엘씨 | 친화성-올리고뉴클레오타이드 콘쥬게이트 및 그것의 사용 |
| EP3452097A1 (en) | 2016-05-04 | 2019-03-13 | Navigo Proteins GmbH | Targeted compounds for the site-specific coupling of chemical moieties comprising a peptide linker |
| SG11201901066UA (en) | 2016-08-11 | 2019-03-28 | Navigo Proteins Gmbh | Novel alkaline stable immunoglobulin-binding proteins |
| BR112019005748A2 (pt) | 2016-09-24 | 2019-06-18 | Abvitro Llc | afinidade-conjugados de oligonucleotídeo e usos destes |
| WO2019091918A1 (en) | 2017-11-07 | 2019-05-16 | Navigo Proteins Gmbh | Fusion proteins with specificity for ed-b and long serum half-life for diagnosis or treatment of cancer |
| MA54501A (fr) | 2018-12-18 | 2021-10-27 | Navigo Proteins Gmbh | Nouvelles protéines de liaison spécifiques de folr1 pour le diagnostic et le traitement du cancer |
| BR112022002365A2 (pt) | 2019-08-09 | 2022-04-26 | Nutcracker Therapeutics Inc | Métodos e aparelhos para fabricar e para remover material de uma composição terapêutica |
| EP4382534A1 (en) | 2022-12-06 | 2024-06-12 | Biomérieux | Synthetic peptide for use in an immunomolecular assay |
| EP4655590A1 (en) | 2023-01-27 | 2025-12-03 | Biomerieux | Modified immuno-molecular assay for faster biomarker detection |
| WO2025111396A1 (en) | 2023-11-20 | 2025-05-30 | Alamar Biosciences, Inc. | Molecular barcode set and use thereof in multiplex proximity detection assays |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06178688A (ja) * | 1992-12-11 | 1994-06-28 | Mitsui Toatsu Chem Inc | E型緑膿菌を抗原とするヒト抗体をコードする遺伝子 |
| AU8124694A (en) | 1993-10-29 | 1995-05-22 | Affymax Technologies N.V. | In vitro peptide and antibody display libraries |
| ES2253358T3 (es) | 2000-03-31 | 2006-06-01 | Cambridge Antibody Technology Limited | Mejoras en la visualizacion del ribosoma. |
-
1998
- 1998-05-28 JP JP50038699A patent/JP4060892B2/ja not_active Expired - Fee Related
- 1998-05-28 WO PCT/GB1998/001564 patent/WO1998054312A1/en not_active Ceased
- 1998-05-28 DE DE69818458T patent/DE69818458T3/de not_active Expired - Lifetime
- 1998-05-28 US US09/424,712 patent/US6620587B1/en not_active Expired - Lifetime
- 1998-05-28 EP EP98924468A patent/EP0985032B2/en not_active Expired - Lifetime
- 1998-05-28 DK DK98924468T patent/DK0985032T4/da active
- 1998-05-28 ES ES98924468T patent/ES2209145T5/es not_active Expired - Lifetime
- 1998-05-28 PT PT98924468T patent/PT985032E/pt unknown
- 1998-05-28 AT AT98924468T patent/ATE250668T1/de active
- 1998-05-28 CA CA2291721A patent/CA2291721C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CA2291721C (en) | 2011-08-09 |
| DE69818458D1 (de) | 2003-10-30 |
| PT985032E (pt) | 2004-02-27 |
| AU725957B2 (en) | 2000-10-26 |
| EP0985032A1 (en) | 2000-03-15 |
| DK0985032T4 (da) | 2009-08-31 |
| ES2209145T5 (es) | 2009-10-30 |
| US6620587B1 (en) | 2003-09-16 |
| CA2291721A1 (en) | 1998-12-03 |
| EP0985032B2 (en) | 2009-06-03 |
| AU7666698A (en) | 1998-12-30 |
| DK0985032T3 (da) | 2004-02-02 |
| WO1998054312A1 (en) | 1998-12-03 |
| DE69818458T2 (de) | 2004-07-08 |
| EP0985032B1 (en) | 2003-09-24 |
| ES2209145T3 (es) | 2004-06-16 |
| ATE250668T1 (de) | 2003-10-15 |
| JP2002500514A (ja) | 2002-01-08 |
| JP4060892B2 (ja) | 2008-03-12 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8363 | Opposition against the patent | ||
| 8366 | Restricted maintained after opposition proceedings | ||
| 8327 | Change in the person/name/address of the patent owner |
Owner name: CRESCENDO BIOLOGICS LTD., BABRAHAM, CAMBRIDGE, GB |
|
| 8328 | Change in the person/name/address of the agent |
Representative=s name: MARKS & CLERK (LUXEMBOURG) LLP, LUXEMBOURG, LU |