CA2291721C - Ribosome complexes as selection particles for in vitro display and evolution of proteins - Google Patents

Ribosome complexes as selection particles for in vitro display and evolution of proteins Download PDF

Info

Publication number
CA2291721C
CA2291721C CA2291721A CA2291721A CA2291721C CA 2291721 C CA2291721 C CA 2291721C CA 2291721 A CA2291721 A CA 2291721A CA 2291721 A CA2291721 A CA 2291721A CA 2291721 C CA2291721 C CA 2291721C
Authority
CA
Canada
Prior art keywords
mrna
protein
peptide
complexed
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2291721A
Other languages
English (en)
French (fr)
Other versions
CA2291721A1 (en
Inventor
Michael John Taussig
Mingyue He
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Crescendo Biologics Ltd
Original Assignee
Crescendo Biologics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27268863&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2291721(C) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from GBGB9710829.4A external-priority patent/GB9710829D0/en
Priority claimed from GBGB9724850.4A external-priority patent/GB9724850D0/en
Priority claimed from GBGB9804195.7A external-priority patent/GB9804195D0/en
Application filed by Crescendo Biologics Ltd filed Critical Crescendo Biologics Ltd
Publication of CA2291721A1 publication Critical patent/CA2291721A1/en
Application granted granted Critical
Publication of CA2291721C publication Critical patent/CA2291721C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1041—Ribosome/Polysome display, e.g. SPERT, ARM
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
CA2291721A 1997-05-28 1998-05-28 Ribosome complexes as selection particles for in vitro display and evolution of proteins Expired - Fee Related CA2291721C (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
GB9710829.4 1997-05-28
GBGB9710829.4A GB9710829D0 (en) 1997-05-28 1997-05-28 Selection particles for in vitro display and evolution of antibody combining sites
GB9724850.4 1997-11-26
GBGB9724850.4A GB9724850D0 (en) 1997-11-26 1997-11-26 Selection particles for in vitro display and evolution of antibody combining sites
GBGB9804195.7A GB9804195D0 (en) 1998-02-28 1998-02-28 Selection particles for in vitro display and evolution of antibody combining sites
GB9804195.7 1998-02-28
PCT/GB1998/001564 WO1998054312A1 (en) 1997-05-28 1998-05-28 Ribosome complexes as selection particles for in vitro display and evolution of proteins

Publications (2)

Publication Number Publication Date
CA2291721A1 CA2291721A1 (en) 1998-12-03
CA2291721C true CA2291721C (en) 2011-08-09

Family

ID=27268863

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2291721A Expired - Fee Related CA2291721C (en) 1997-05-28 1998-05-28 Ribosome complexes as selection particles for in vitro display and evolution of proteins

Country Status (10)

Country Link
US (1) US6620587B1 (https=)
EP (1) EP0985032B2 (https=)
JP (1) JP4060892B2 (https=)
AT (1) ATE250668T1 (https=)
CA (1) CA2291721C (https=)
DE (1) DE69818458T3 (https=)
DK (1) DK0985032T4 (https=)
ES (1) ES2209145T5 (https=)
PT (1) PT985032E (https=)
WO (1) WO1998054312A1 (https=)

Families Citing this family (76)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU4854999A (en) * 1998-07-02 2000-01-24 Invitro Diagnostics, Inc. Methods, compositions and apparatus for making nucleic acid molecules having a selected affinity to a target molecule
DE19932688B4 (de) 1999-07-13 2009-10-08 Scil Proteins Gmbh Design von Beta-Faltblatt-Proteinen des gamma-II-kristallins antikörperähnlichen
GB9917027D0 (en) 1999-07-20 1999-09-22 Affibody Technology Sweeden Ab In vitro selection and optional identification of polypeptides using solid support carriers
IL150291A0 (en) * 2000-01-11 2002-12-01 Maxygen Inc Integrated systems and methods for diversity generation and screening
EP1652919A1 (en) * 2000-03-31 2006-05-03 Cambridge Antibody Technology LTD Improvements to ribosome display
CA2402452A1 (en) * 2000-03-31 2001-10-11 Cambridge Antibody Technology Limited Improvements to ribosome display
US20020098524A1 (en) 2000-04-14 2002-07-25 Murray Christopher J. Methods for selective targeting
US7129326B2 (en) 2000-04-14 2006-10-31 Genencor International, Inc. Methods for selective targeting
EP1372721A4 (en) 2001-03-07 2008-04-02 Childrens Medical Center METHOD FOR SCREENING PEPTIDE DISPLAY LIBRARIES WITH A MINIMUM DISPLAY
US20060234231A1 (en) * 2001-06-20 2006-10-19 Nuevolution A/S Microarrays displaying encoded molecules
EP1401850A1 (en) 2001-06-20 2004-03-31 Nuevolution A/S Nucleoside derivatives for library preparation
DE10145226A1 (de) * 2001-09-13 2003-04-10 Lifebits Ag Herstellung von trägergebundenen Molekülen
DE10147074A1 (de) * 2001-09-25 2003-05-08 Beru Ag Verfahren zum Betreiben einer aus mehreren Heizelementen bestehenden mehrstufigen elektrischen Heizung
DE20200926U1 (de) * 2002-01-23 2002-04-18 Hegenscheidt-MFD GmbH & Co. KG, 41812 Erkelenz Festwalzgerät einer Festwalzmaschine für Kurbelwellen
US10731151B2 (en) 2002-03-15 2020-08-04 Nuevolution A/S Method for synthesising templated molecules
US20050247001A1 (en) * 2002-03-15 2005-11-10 Nuevolution A/S Building block forming a c-c or a c-hetero atom bond uponreaction
AU2003214031A1 (en) 2002-03-15 2003-09-29 Nuevolution A/S An improved method for synthesising templated molecules
WO2003078050A2 (en) * 2002-03-15 2003-09-25 Nuevolution A/S A building block forming a c-c bond upon reaction
WO2003078627A2 (en) * 2002-03-15 2003-09-25 Nuevolution A/S A building block capable of functional entity transfer to nucleophil
EP1539980B1 (en) * 2002-08-01 2016-02-17 Nuevolution A/S Library of complexes comprising small non-peptide molecules and double-stranded oligonucleotides identifying the molecules
DK1534830T3 (da) * 2002-09-06 2011-02-14 Isogenica Ltd In vito-peptid-ekspressionsbibliotek
EP2348124B1 (en) * 2002-10-30 2013-12-11 Nuevolution A/S Synthesis of a bifunctional complex
EP2581383A1 (en) 2002-11-25 2013-04-17 Genencor International, Inc. Skin or hair binding peptides
WO2004056994A2 (en) * 2002-12-19 2004-07-08 Nuevolution A/S Quasirandom structure and function guided synthesis methods
US20070026397A1 (en) 2003-02-21 2007-02-01 Nuevolution A/S Method for producing second-generation library
WO2004074501A2 (en) * 2003-02-21 2004-09-02 Nuevolution A/S A method for obtaining structural information about an encoded molecule
ES2326649T3 (es) * 2003-03-19 2009-10-16 Crescendo Biologics Ltd. Recuperacion de dna.
US7915201B2 (en) * 2003-03-20 2011-03-29 Nuevolution A/S Ligational encoding of small molecules
DE10324447A1 (de) 2003-05-28 2004-12-30 Scil Proteins Gmbh Generierung künstlicher Bindungsproteine auf der Grundlage von Ubiquitin
GB0316314D0 (en) * 2003-07-12 2003-08-13 Univ Belfast Method
US11118215B2 (en) * 2003-09-18 2021-09-14 Nuevolution A/S Method for obtaining structural information concerning an encoded molecule and method for selecting compounds
WO2005078122A2 (en) * 2004-02-17 2005-08-25 Nuevolution A/S Method for enrichment involving elimination by mismatch hybridisation
DE602005017370D1 (de) * 2004-03-22 2009-12-10 Nuevolution As Ligationscodierung unter verwendung von oligonukleotidbausteinen
US20050250112A1 (en) * 2004-05-07 2005-11-10 Padmabandu Gothami A Nucleic acids, compositions, methods, and kits for detecting mycoplasma and acholeplasma species
US9006393B1 (en) 2004-08-26 2015-04-14 Patricia L. Clark Molecular constructs and uses thereof in ribosomal translational events
DE102004049479A1 (de) 2004-10-11 2006-04-13 Scil Proteins Gmbh Proteinkonjugate zur Verwendung in Therapie, Diagnose und Chromatographie
PT3305900T (pt) 2005-12-01 2021-10-27 Nuevolution As Métodos de codificação enzimática para síntese eficaz de bibliotecas grandes
GB0605584D0 (en) 2006-03-20 2006-04-26 Olink Ab Method for analyte detection using proximity probes
US7923533B2 (en) * 2006-06-29 2011-04-12 The Invention Science Fund I, Llc Methods for arbitrary peptide synthesis
US7910695B2 (en) 2006-06-29 2011-03-22 The Invention Science Fund I, Llc Methods for arbitrary peptide synthesis
US7879975B2 (en) * 2006-06-29 2011-02-01 The Invention Science Fund I, Llc Methods for arbitrary peptide synthesis
US7993873B2 (en) * 2006-06-29 2011-08-09 The Invention Science Fund I, Llc Apparatus for arbitrary peptide synthesis
US7777002B2 (en) * 2006-06-29 2010-08-17 The Invention Science Fund I, Llc Methods for arbitrary peptide synthesis
EP1925664A1 (en) 2006-11-15 2008-05-28 Scil proteins GmbH Artificial binding proteins based on a modified alpha helical region of ubiquitin
US8865179B2 (en) * 2008-01-26 2014-10-21 Swey-Shen Alexchen Aptameric IgE peptides in a protein scaffold as an allergy vaccine
EP2316030B1 (en) 2008-07-25 2019-08-21 Wagner, Richard W. Protein screeing methods
KR20110076906A (ko) * 2008-09-30 2011-07-06 아보트 러보러터리즈 개선된 ｒｎａ 디스플레이 방법
RU2553333C2 (ru) 2009-12-14 2015-06-10 Сцил Протеинс Гмбх Способ идентификации гетеромультимерных модифицированных убиквитиновых белков со способностью связываться с лигандами
WO2011111832A1 (ja) 2010-03-11 2011-09-15 独立行政法人理化学研究所 ポリペプチド配列の選択方法、並びに金属酸化物または含珪素化合物結合ペプチドおよびその利用
GB201004292D0 (en) 2010-03-15 2010-04-28 Olink Ab Assay for localised detection of analytes
EP3540059A1 (en) 2010-04-16 2019-09-18 Nuevolution A/S Bi-functional complexes and methods for making and using such complexes
GB201011971D0 (en) 2010-07-15 2010-09-01 Olink Ab Methods and product
US10669569B2 (en) 2010-10-15 2020-06-02 Navinci Diagnostics Ab Dynamic range methods
EP2466310A1 (en) 2010-12-17 2012-06-20 Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt GmbH Means and methods for detecting double-strand breaks
DE102010056289A1 (de) 2010-12-24 2012-06-28 Geneart Ag Verfahren zur Herstellung von Leseraster-korrekten Fragment-Bibliotheken
GB201101621D0 (en) 2011-01-31 2011-03-16 Olink Ab Method and product
JP5948673B2 (ja) 2011-03-10 2016-07-06 ジーンフロンティア株式会社 リポ多糖混入量を低減した蛋白質合成用組成物、該組成物を用いた蛋白質製造方法
GB201107863D0 (en) 2011-05-11 2011-06-22 Olink Ab Method and product
JP6057297B2 (ja) 2011-05-23 2017-01-11 国立研究開発法人理化学研究所 核酸構築物、核酸−蛋白質複合体、及びその利用
CA2837804C (en) 2011-06-15 2018-03-20 Scil Proteins Gmbh Dimeric binding proteins based on modified ubiquitins
GB201201547D0 (en) 2012-01-30 2012-03-14 Olink Ab Method and product
WO2015120058A2 (en) 2014-02-05 2015-08-13 Molecular Templates, Inc. Methods of screening, selecting, and identifying cytotoxic recombinant polypeptides based on an interim diminution of ribotoxicity
EP3209776A1 (en) * 2014-10-22 2017-08-30 Crescendo Biologics Limited Human vh domain scaffolds
JP6738340B2 (ja) 2015-02-06 2020-08-12 ナフィゴ プロテインズ ゲゼルシャフト ミット ベシュレンクテル ハフツングＮａｖｉｇｏ Ｐｒｏｔｅｉｎｓ ＧｍｂＨ 新規なｅｇｆｒ結合タンパク質
JP6856938B2 (ja) 2015-07-16 2021-04-14 ナフィゴ プロテインズ ゲゼルシャフト ミット ベシュレンクテル ハフツングＮａｖｉｇｏ Ｐｒｏｔｅｉｎｓ ＧｍｂＨ 新規な免疫グロブリン結合タンパク質およびアフィニティ精製におけるそれらの使用
WO2017013136A1 (en) 2015-07-20 2017-01-26 Scil Proteins Gmbh Novel binding proteins based on di-ubiquitin muteins and methods for generation
MX2018003532A (es) 2015-09-24 2019-04-25 Abvitro Llc Conjugados de afinidad-oligonucleotido y usos de los mismos.
US11813336B2 (en) 2016-05-04 2023-11-14 Navigo Proteins Gmbh Targeted compounds for the site-specific coupling of chemical moieties comprising a peptide linker
CN109963864B (zh) 2016-08-11 2024-01-02 瑞普利金公司 用于亲和色谱的碱性稳定性fc结合蛋白
JP6929354B2 (ja) 2016-09-24 2021-09-01 アブビトロ， エルエルシー 親和性−オリゴヌクレオチドコンジュゲートおよびその使用
US11414466B2 (en) 2017-11-07 2022-08-16 Navigo Proteins Gmbh Fusion proteins with specificity for ED-B and long serum half-life for diagnosis or treatment of cancer
MX2021007283A (es) 2018-12-18 2021-07-15 Navigo Proteins Gmbh Proteinas de union especificas del folr1 novedosas para diagnostico y tratamiento del cancer.
MX2022001664A (es) 2019-08-09 2022-07-19 Nutcracker Therapeutics Inc Aparato microfluídico y metodos de uso del mismo.
EP4382534A1 (en) 2022-12-06 2024-06-12 Biomérieux Synthetic peptide for use in an immunomolecular assay
EP4655590A1 (en) 2023-01-27 2025-12-03 Biomerieux Modified immuno-molecular assay for faster biomarker detection
WO2025111396A1 (en) 2023-11-20 2025-05-30 Alamar Biosciences, Inc. Molecular barcode set and use thereof in multiplex proximity detection assays

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH06178688A (ja) 1992-12-11 1994-06-28 Mitsui Toatsu Chem Inc Ｅ型緑膿菌を抗原とするヒト抗体をコードする遺伝子
WO1995011922A1 (en) 1993-10-29 1995-05-04 Affymax Technologies N.V. In vitro peptide and antibody display libraries
CA2402452A1 (en) 2000-03-31 2001-10-11 Cambridge Antibody Technology Limited Improvements to ribosome display

Also Published As

Publication number Publication date
ES2209145T5 (es) 2009-10-30
AU725957B2 (en) 2000-10-26
DK0985032T4 (da) 2009-08-31
JP4060892B2 (ja) 2008-03-12
CA2291721A1 (en) 1998-12-03
US6620587B1 (en) 2003-09-16
WO1998054312A1 (en) 1998-12-03
DE69818458D1 (de) 2003-10-30
DK0985032T3 (da) 2004-02-02
DE69818458T2 (de) 2004-07-08
DE69818458T3 (de) 2009-12-17
EP0985032B2 (en) 2009-06-03
JP2002500514A (ja) 2002-01-08
ATE250668T1 (de) 2003-10-15
EP0985032B1 (en) 2003-09-24
AU7666698A (en) 1998-12-30
EP0985032A1 (en) 2000-03-15
PT985032E (pt) 2004-02-27
ES2209145T3 (es) 2004-06-16

Similar Documents

Publication Publication Date Title
CA2291721C (en) Ribosome complexes as selection particles for in vitro display and evolution of proteins
Irving et al. Ribosome display and affinity maturation: from antibodies to single V-domains and steps towards cancer therapeutics
Schaffitzel et al. Ribosome display: an in vitro method for selection and evolution of antibodies from libraries
JP5968624B2 (ja) 改良ｒｎａディスプレイ法
US5922545A (en) In vitro peptide and antibody display libraries
He et al. Ribosome display: cell-free protein display technology
AU2001239444B2 (en) Improvements to ribosome display
EP0975748A1 (en) Methods dor identifying nucleic acid molecules encoding (poly)peptides that interact with target molecules
He et al. Ribosome display of antibodies: expression, specificity and recovery in a eukaryotic system
AU2001239444A1 (en) Improvements to ribosome display
JP2023518900A (ja) 抗原特異的結合ポリペプチド遺伝子ディスプレイベクターの構築方法および用途
AU2003253193B2 (en) Novel tricistronic vectors and uses therefor
EP2356231A2 (en) Compatible display vector systems
WO2010036856A2 (en) Compatible display vector systems
AU725957C (en) Ribosome complexes as selection particles for in vitro display and evolution of proteins
WO2004083428A1 (en) In vitro cloning methods
EP1652919A1 (en) Improvements to ribosome display
HK1159250B (en) Improved method of rna display

Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed

Effective date: 20170529