DE69736391T2 - Verfahren zur herstellung eines rekombinanten polypeptids unter hinzufügung eines hemmstoffes einer metallabhängigen protease oder chymotrypsins zum zellkulturmedium - Google Patents
Verfahren zur herstellung eines rekombinanten polypeptids unter hinzufügung eines hemmstoffes einer metallabhängigen protease oder chymotrypsins zum zellkulturmedium Download PDFInfo
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- DE69736391T2 DE69736391T2 DE69736391T DE69736391T DE69736391T2 DE 69736391 T2 DE69736391 T2 DE 69736391T2 DE 69736391 T DE69736391 T DE 69736391T DE 69736391 T DE69736391 T DE 69736391T DE 69736391 T2 DE69736391 T2 DE 69736391T2
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- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- 229960002743 glutamine Drugs 0.000 description 1
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- WQPDUTSPKFMPDP-OUMQNGNKSA-N hirudin Chemical compound C([C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1C=CC(OS(O)(=O)=O)=CC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CCCCN)NC(=O)[C@H]1N(CCC1)C(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H]1NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)CC)NC(=O)[C@@H]2CSSC[C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@H](C(=O)N[C@H](C(NCC(=O)N[C@@H](CCC(N)=O)C(=O)NCC(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCCN)C(=O)N2)=O)CSSC1)C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]1NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)CNC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=2C=CC(O)=CC=2)NC(=O)[C@@H](NC(=O)[C@@H](N)C(C)C)C(C)C)[C@@H](C)O)CSSC1)C(C)C)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 WQPDUTSPKFMPDP-OUMQNGNKSA-N 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/745—Blood coagulation or fibrinolysis factors
- C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Hematology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pharmacology & Pharmacy (AREA)
- Toxicology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Diabetes (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Plant Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- Physics & Mathematics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE9601855A SE9601855D0 (sv) | 1996-05-14 | 1996-05-14 | Process for producing a protein |
| SE9601855 | 1996-05-14 | ||
| US1887496P | 1996-05-29 | 1996-05-29 | |
| US18874P | 1996-05-29 | ||
| PCT/SE1997/000783 WO1997043436A1 (en) | 1996-05-14 | 1997-05-13 | A process for producing a recombinant polypeptide involving the addition of an inhibitor of metal-dependent proteases or chymotrypsins to the cell culture medium |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69736391D1 DE69736391D1 (de) | 2006-09-07 |
| DE69736391T2 true DE69736391T2 (de) | 2007-08-16 |
Family
ID=26662616
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69736391T Expired - Lifetime DE69736391T2 (de) | 1996-05-14 | 1997-05-13 | Verfahren zur herstellung eines rekombinanten polypeptids unter hinzufügung eines hemmstoffes einer metallabhängigen protease oder chymotrypsins zum zellkulturmedium |
| DE69735510T Expired - Lifetime DE69735510T2 (de) | 1996-05-14 | 1997-05-13 | Verfahren zur Herstellung eines rekombinanten Polypeptids unter Hinzufügung eines Chymotrypsinhemmstoffes zum Zellkulturmedium |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69735510T Expired - Lifetime DE69735510T2 (de) | 1996-05-14 | 1997-05-13 | Verfahren zur Herstellung eines rekombinanten Polypeptids unter Hinzufügung eines Chymotrypsinhemmstoffes zum Zellkulturmedium |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US5851800A (enExample) |
| EP (1) | EP0934424B1 (enExample) |
| JP (2) | JP2000509994A (enExample) |
| AT (2) | ATE321141T1 (enExample) |
| AU (1) | AU716245B2 (enExample) |
| CA (1) | CA2253287C (enExample) |
| DE (2) | DE69736391T2 (enExample) |
| DK (1) | DK0934424T3 (enExample) |
| ES (2) | ES2270460T3 (enExample) |
| NO (2) | NO325358B1 (enExample) |
| NZ (1) | NZ332751A (enExample) |
| PT (2) | PT1318198E (enExample) |
| WO (1) | WO1997043436A1 (enExample) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6475725B1 (en) | 1997-06-20 | 2002-11-05 | Baxter Aktiengesellschaft | Recombinant cell clones having increased stability and methods of making and using the same |
| CZ307715B6 (cs) | 1999-02-22 | 2019-03-06 | University Of Connecticut | Způsob lyofilizace vodné farmaceutické formulace faktoru VIII prosté albuminu |
| AT409379B (de) | 1999-06-02 | 2002-07-25 | Baxter Ag | Medium zur protein- und serumfreien kultivierung von zellen |
| WO2001011021A1 (de) * | 1999-08-05 | 2001-02-15 | Baxter Aktiengesellschaft | Rekombinanter stabiler zellklon, seine herstellung und verwendung |
| WO2001052891A1 (fr) * | 2000-01-20 | 2001-07-26 | Nippon Organon K. K. | Inhibiteurs d'invasion plasmodiale dans des erythrocytes |
| DK2287288T3 (da) * | 2002-07-09 | 2013-01-07 | Baxter Int | Medium frit for animalsk protein til dyrkning af celler |
| PL377731A1 (pl) * | 2002-12-23 | 2006-02-06 | Bristol-Myers Squibb Company | Sposoby hodowli komórek ssaczych do wytwarzania białka |
| WO2004058944A2 (en) * | 2002-12-23 | 2004-07-15 | Bristol-Myers Squibb Company | Product quality enhancement in mammalian cell culture processes for protein production |
| JP2007525176A (ja) * | 2003-04-25 | 2007-09-06 | イミュネックス・コーポレーション | 組換えタンパク質発現の誘導剤 |
| US7255288B2 (en) * | 2004-03-08 | 2007-08-14 | Wan Shan Chan | Aroma therapy for fountain |
| US20060094104A1 (en) | 2004-10-29 | 2006-05-04 | Leopold Grillberger | Animal protein-free media for cultivation of cells |
| EP1707634A1 (en) * | 2005-03-29 | 2006-10-04 | Octapharma AG | Method for isolation of recombinantly produced proteins |
| EP3653699A1 (en) | 2006-01-04 | 2020-05-20 | Baxalta Incorporated | Oligopeptide-free cell culture media |
| LT2126106T (lt) | 2007-02-23 | 2018-01-10 | Sk Chemicals Co., Ltd. | Viii faktoriaus ir jo darinių gavimo ir gryninimo būdas |
| WO2008135498A2 (en) * | 2007-05-04 | 2008-11-13 | Novo Nordisk A/S | Prevention of protein degradation in mammalian cell cultures |
| EP1988101A1 (en) | 2007-05-04 | 2008-11-05 | Novo Nordisk A/S | Improvement of factor VIII polypeptide titers in cell cultures |
| KR101752508B1 (ko) | 2008-11-07 | 2017-06-29 | 백스터 인터내셔널 인코포레이티드 | 제8 인자 제형 |
| US9540426B2 (en) | 2009-10-06 | 2017-01-10 | Bristol-Myers Squibb Company | Mammalian cell culture processes for protein production |
| EP2625262B1 (en) | 2010-10-05 | 2015-09-23 | Novo Nordisk Health Care AG | Process for protein production |
| WO2014062535A1 (en) | 2012-10-15 | 2014-04-24 | Bristol-Myers Squibb Company | Mammalian cell culture processes for protein production |
| KR102670157B1 (ko) | 2015-10-28 | 2024-05-29 | 주식회사유한양행 | 이중 작용 단백질 및 이를 포함하는 약학적 조성물 |
| KR102668200B1 (ko) | 2015-10-28 | 2024-05-23 | 주식회사유한양행 | 지속형 fgf21 융합 단백질 및 이를 포함하는 약학적 조성물 |
| EP3568149B1 (en) | 2016-11-10 | 2025-03-12 | Yuhan Corporation | Pharmaceutical composition for preventing or treating hepatitis, hepatic fibrosis, and hepatic cirrhosis comprising fusion proteins |
| BR112019021923A2 (pt) | 2017-04-21 | 2020-06-02 | Yuhan Corporation | Método para produzir proteínas de função dupla e suas derivadas |
Family Cites Families (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5149787A (en) * | 1984-05-22 | 1992-09-22 | The Blood Center Research Foundation | Method for maintaining intact, non-degraded factor VIII/von-Willebrand factor during blood processing |
| SE8501050D0 (sv) * | 1985-03-05 | 1985-03-05 | Kabivitrum Ab | Biologically active fragments of human antihemophilic factor and method for preparation thereof |
| US5279939A (en) * | 1986-08-18 | 1994-01-18 | Smithkline Beecham Corporation | Protein protease inhibitors from streptomyces |
| US4891356A (en) * | 1987-07-15 | 1990-01-02 | Brigham & Women's Hospital | Proteinase inhibitors for treatment of gastrointestinal ulcer disease |
| JPH0387173A (ja) * | 1987-09-10 | 1991-04-11 | Teijin Ltd | ヒト活性化天然型ファクター８ｃの製造方法及びそれに用いる形質転換体 |
| JP2769170B2 (ja) * | 1987-12-08 | 1998-06-25 | ザイモジェネティクス，インコーポレイティド | 真核細肪中での同時発現 |
| CA1340740C (en) * | 1987-12-08 | 1999-09-14 | Eileen R. Mulvihill | Co-expression in eukaryotic cells |
| DK457788D0 (da) * | 1988-08-16 | 1988-08-16 | Nordisk Gentofte | Fremgangsmaade til fremstilling af et oensket polypeptid |
| WO1990002175A1 (en) * | 1988-08-16 | 1990-03-08 | Novo Nordisk A/S | A method of producing polypeptides by culturing eukaryotic cells in the presence of protease inhibitors |
| GB8827305D0 (en) * | 1988-11-23 | 1988-12-29 | British Bio Technology | Compounds |
| DK105489D0 (da) * | 1989-03-03 | 1989-03-03 | Novo Nordisk As | Polypeptid |
| SE465222C5 (sv) * | 1989-12-15 | 1998-02-10 | Pharmacia & Upjohn Ab | Ett rekombinant, humant faktor VIII-derivat och förfarande för dess framställning |
| WO1991013167A1 (en) * | 1990-02-26 | 1991-09-05 | The Board Of Trustees Of The Leland Stanford Junior University | Identification and expression of insect steroid receptor dna sequences |
| US5189178A (en) * | 1990-11-21 | 1993-02-23 | Galardy Richard E | Matrix metalloprotease inhibitors |
| US5304603A (en) * | 1991-06-18 | 1994-04-19 | The Population Council | Leydig cell stimulator |
| US5661034A (en) * | 1991-07-18 | 1997-08-26 | Fuji Yakuhin Kogyo Kabushiki Kaisha | Serum-free medium for tissue culture containing tissue inhibitor of metalloproteinases and method for growing cells using the medium |
| US7018809B1 (en) * | 1991-09-19 | 2006-03-28 | Genentech, Inc. | Expression of functional antibody fragments |
| CA2078721A1 (en) * | 1991-09-24 | 1993-03-25 | Hiroshi Yonemura | Process for preparing human coagulation factor viii protein complex |
| US5278289A (en) * | 1991-11-12 | 1994-01-11 | Johnson Alan J | Antihemophilic factor stabilization |
| US5296471A (en) * | 1992-12-22 | 1994-03-22 | Glycomed Incorporated | Method for controlling o-desulfation of heparin and compositions produced thereby |
| AU7524994A (en) * | 1993-08-12 | 1995-03-14 | University Of Maryland | Thermostable alkaline metalloprotease produced by a hyphomonas, and preparation thereof |
| US5631159A (en) * | 1993-09-22 | 1997-05-20 | Creative Biomolecules, Inc. | Lipid-modified serum free media |
| US5543396A (en) * | 1994-04-28 | 1996-08-06 | Georgia Tech Research Corp. | Proline phosphonate derivatives |
-
1997
- 1997-05-07 US US08/852,783 patent/US5851800A/en not_active Expired - Lifetime
- 1997-05-13 AT AT03000391T patent/ATE321141T1/de active
- 1997-05-13 PT PT03000391T patent/PT1318198E/pt unknown
- 1997-05-13 DK DK97923382T patent/DK0934424T3/da active
- 1997-05-13 DE DE69736391T patent/DE69736391T2/de not_active Expired - Lifetime
- 1997-05-13 WO PCT/SE1997/000783 patent/WO1997043436A1/en not_active Ceased
- 1997-05-13 NZ NZ332751A patent/NZ332751A/en not_active IP Right Cessation
- 1997-05-13 DE DE69735510T patent/DE69735510T2/de not_active Expired - Lifetime
- 1997-05-13 ES ES97923382T patent/ES2270460T3/es not_active Expired - Lifetime
- 1997-05-13 CA CA002253287A patent/CA2253287C/en not_active Expired - Fee Related
- 1997-05-13 ES ES03000391T patent/ES2260524T3/es not_active Expired - Lifetime
- 1997-05-13 AU AU29197/97A patent/AU716245B2/en not_active Ceased
- 1997-05-13 PT PT97923382T patent/PT934424E/pt unknown
- 1997-05-13 AT AT97923382T patent/ATE334223T1/de active
- 1997-05-13 EP EP97923382A patent/EP0934424B1/en not_active Expired - Lifetime
- 1997-05-13 JP JP09540800A patent/JP2000509994A/ja active Pending
-
1998
- 1998-11-13 NO NO19985297A patent/NO325358B1/no not_active IP Right Cessation
-
2006
- 2006-11-02 NO NO20065027A patent/NO325633B1/no not_active IP Right Cessation
-
2009
- 2009-01-07 JP JP2009001597A patent/JP4896999B2/ja not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| NO20065027L (no) | 1999-01-14 |
| PT934424E (pt) | 2006-12-29 |
| NZ332751A (en) | 2000-06-23 |
| ATE321141T1 (de) | 2006-04-15 |
| NO985297L (no) | 1999-01-14 |
| DE69735510D1 (de) | 2006-05-11 |
| EP0934424A1 (en) | 1999-08-11 |
| WO1997043436A1 (en) | 1997-11-20 |
| AU716245B2 (en) | 2000-02-24 |
| JP2000509994A (ja) | 2000-08-08 |
| PT1318198E (pt) | 2006-08-31 |
| JP4896999B2 (ja) | 2012-03-14 |
| DE69735510T2 (de) | 2006-08-24 |
| NO325633B1 (no) | 2008-06-30 |
| ES2260524T3 (es) | 2006-11-01 |
| ES2270460T3 (es) | 2007-04-01 |
| DK0934424T3 (da) | 2006-11-20 |
| CA2253287A1 (en) | 1997-11-20 |
| EP0934424B1 (en) | 2006-07-26 |
| NO985297D0 (no) | 1998-11-13 |
| ATE334223T1 (de) | 2006-08-15 |
| AU2919797A (en) | 1997-12-05 |
| US5851800A (en) | 1998-12-22 |
| DE69736391D1 (de) | 2006-09-07 |
| JP2009148271A (ja) | 2009-07-09 |
| CA2253287C (en) | 2008-02-19 |
| NO325358B1 (no) | 2008-04-07 |
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| 8364 | No opposition during term of opposition |