DE69728377T2 - Verbesserte mutanten von (2,5 dkg) reduktase a - Google Patents

Verbesserte mutanten von (2,5 dkg) reduktase a Download PDF

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Publication number
DE69728377T2
DE69728377T2 DE69728377T DE69728377T DE69728377T2 DE 69728377 T2 DE69728377 T2 DE 69728377T2 DE 69728377 T DE69728377 T DE 69728377T DE 69728377 T DE69728377 T DE 69728377T DE 69728377 T2 DE69728377 T2 DE 69728377T2
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DE
Germany
Prior art keywords
dkg
reductase
mutant
dkg reductase
seq
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69728377T
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German (de)
English (en)
Other versions
DE69728377D1 (de
Inventor
B. David POWERS
Stephen Anderson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rutgers State University of New Jersey
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Rutgers State University of New Jersey
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rutgers State University of New Jersey filed Critical Rutgers State University of New Jersey
Application granted granted Critical
Publication of DE69728377D1 publication Critical patent/DE69728377D1/de
Publication of DE69728377T2 publication Critical patent/DE69728377T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/843—Corynebacterium

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
DE69728377T 1996-01-11 1997-01-09 Verbesserte mutanten von (2,5 dkg) reduktase a Expired - Fee Related DE69728377T2 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US58401996A 1996-01-11 1996-01-11
US584019 1996-01-11
US08/585,595 US5795761A (en) 1996-01-11 1996-01-16 Mutants of 2,5-diketo-D-gluconic acid (2,5-DKG) reductase A
US585595 1996-01-16
PCT/US1997/000097 WO1997025432A2 (en) 1996-01-11 1997-01-09 Improved mutants of (2,5 dkg) reductase

Publications (2)

Publication Number Publication Date
DE69728377D1 DE69728377D1 (de) 2004-05-06
DE69728377T2 true DE69728377T2 (de) 2005-01-20

Family

ID=27078962

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69728377T Expired - Fee Related DE69728377T2 (de) 1996-01-11 1997-01-09 Verbesserte mutanten von (2,5 dkg) reduktase a

Country Status (12)

Country Link
US (1) US5795761A (es)
EP (1) EP0873411B1 (es)
JP (1) JP3880624B2 (es)
AT (1) ATE263245T1 (es)
AU (1) AU719516B2 (es)
CA (1) CA2242965A1 (es)
DE (1) DE69728377T2 (es)
DK (1) DK0873411T3 (es)
ES (1) ES2218659T3 (es)
NZ (1) NZ331000A (es)
PT (1) PT873411E (es)
WO (1) WO1997025432A2 (es)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6946280B1 (en) * 1996-03-29 2005-09-20 Genencor International, Inc. Enzyme multimer and process of producing same
US5919685A (en) * 1997-08-08 1999-07-06 Incyte Pharmaceuticals, Inc. Human aflatoxin B1 aldehyde reductase
CN1119414C (zh) * 1997-12-30 2003-08-27 中国科学院上海生物工程研究中心 突变的2,5二酮基－d－葡萄糖酸还原酶及其基因工程表达
US6599722B2 (en) * 1998-12-22 2003-07-29 Genencor International, Inc. Method for producing ascorbic acid intermediates
US7256027B1 (en) * 1999-06-15 2007-08-14 Rutgers, The State University Of New Jersey Enzymes for the production of 2-keto-L-gulonic acid
WO2001059163A2 (fr) * 2000-02-08 2001-08-16 Aventis Pharma S.A. Procede de detection et de quantification d'adenovirus
US6720168B2 (en) 2000-08-04 2004-04-13 Genencor International, Inc. 2,5-DKG permeases
CA2417969A1 (en) * 2000-08-04 2002-02-14 Genecor International, Inc. Enhancement of industrial production by increasing substrate transport
US6423518B1 (en) 2000-09-11 2002-07-23 Rutgers, The State University Design and production of mutant 2,5-diketo-D-gluconic acid reductase enzymes with altered cofactor dependency
US6576452B1 (en) * 2000-10-04 2003-06-10 Genencor International, Inc. 2,5-diketo-L-gluconic acid reductases and methods of use
AU2002314738A1 (en) 2001-04-04 2002-10-21 Genencor International, Inc. Methods for the production of products in host cells
CA2443145C (en) * 2001-04-04 2013-01-22 Genencor International, Inc. Uncoupled productive and catabolic host cell pathways
US7229811B2 (en) 2001-08-03 2007-06-12 Genencor International, Inc. 2,5-diketo-D-gluconic acid (2,5-DKG) permeases
MXPA05012413A (es) * 2003-05-22 2006-02-13 Genencor Int Cepas bacterianas disenadas metabolicamente que tienen transportadores de gluconato endogenos no funcionales.
US20050080248A1 (en) 2003-07-30 2005-04-14 Caldwell Robert M. Multimeric oxidoreductases
EP1649030B1 (en) * 2003-07-30 2014-06-25 Danisco US Inc. Metabolically engineered bacterial strains having enhanced 2-keto-d-gluconate accumulation
EP2459720B1 (en) * 2009-07-30 2016-04-27 Metabolic Explorer Mutant glycerol dehydrogenase (glydh) for the production of a biochemical by fermentation
CN113801872A (zh) * 2021-10-11 2021-12-17 杭州师范大学 一种精准调控组装制备稳定酶蛋白环的方法

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5021559B2 (es) * 1973-03-22 1975-07-23
JPS5135487A (en) * 1974-09-20 1976-03-25 Shionogi Seiyaku Kk 22 keto ll guronsan no seizohoho
JPS5135485A (en) * 1974-09-20 1976-03-25 Shionogi Seiyaku Kk 22 keto ll guronsan no seizohoho
JPS6041596B2 (ja) * 1980-08-14 1985-09-18 塩野義製薬株式会社 ２，５−ジケト−ｄ−グルコン酸の製造方法
JPS58162298A (ja) * 1982-03-05 1983-09-26 Shionogi & Co Ltd ２−ケト−ｌ−グロン酸の製造方法
US4543331A (en) * 1982-03-05 1985-09-24 Shionogi & Co., Ltd. Fermentative or enzymatic production of 2-keto-L-gulonic acid
US4760025A (en) * 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
US4758514A (en) * 1983-06-28 1988-07-19 Genentech, Inc. Ascorbic acid intermediates and process enzymes
US5004690A (en) * 1983-06-28 1991-04-02 Genetech, Inc. Ascorbic acid intermediates and process enzymes
US4757012A (en) * 1983-06-28 1988-07-12 Genentech, Inc. Ascorbic acid intermediates and process enzymes
JPS60118186A (ja) * 1983-11-17 1985-06-25 Shionogi & Co Ltd ２・５−ジケト−ｄ−グルコン酸リダクタ−ゼ
US5008193A (en) * 1984-06-14 1991-04-16 Genentech, Inc. Ascorbic acid intermediates and process enzymes
WO1987000863A1 (en) * 1985-08-02 1987-02-12 Biogen N.V. Production of a vitamin c precursor using genetically modified organisms
GB8519536D0 (en) * 1985-08-02 1985-09-11 Biogen Nv Vitamin c precursor
US5017691A (en) * 1986-07-03 1991-05-21 Schering Corporation Mammalian interleukin-4
US4908773A (en) * 1987-04-06 1990-03-13 Genex Corporation Computer designed stabilized proteins and method for producing same
US4704692A (en) * 1986-09-02 1987-11-03 Ladner Robert C Computer based system and method for determining and displaying possible chemical structures for converting double- or multiple-chain polypeptides to single-chain polypeptides
US4853871A (en) * 1987-04-06 1989-08-01 Genex Corporation Computer-based method for designing stablized proteins
US5032514A (en) * 1988-08-08 1991-07-16 Genentech, Inc. Metabolic pathway engineering to increase production of ascorbic acid intermediates
US5376544A (en) * 1992-09-08 1994-12-27 Rutgers The State University Of New Jersey Enzymes for the production of 2-keto-L-gulonic acid

Also Published As

Publication number Publication date
ATE263245T1 (de) 2004-04-15
PT873411E (pt) 2004-08-31
JPH11514530A (ja) 1999-12-14
EP0873411B1 (en) 2004-03-31
JP3880624B2 (ja) 2007-02-14
US5795761A (en) 1998-08-18
AU719516B2 (en) 2000-05-11
NZ331000A (en) 1999-11-29
ES2218659T3 (es) 2004-11-16
WO1997025432A3 (en) 1997-11-06
DK0873411T3 (da) 2004-07-19
DE69728377D1 (de) 2004-05-06
WO1997025432A2 (en) 1997-07-17
CA2242965A1 (en) 1997-07-17
EP0873411A1 (en) 1998-10-28
AU1691197A (en) 1997-08-01

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8339 Ceased/non-payment of the annual fee