DE69728141T2 - Verfahren zur herstellung eines porösen vernetzten polysaccharidgels und seine verwendung als filtermedien und in der chromatographie - Google Patents
Verfahren zur herstellung eines porösen vernetzten polysaccharidgels und seine verwendung als filtermedien und in der chromatographie Download PDFInfo
- Publication number
- DE69728141T2 DE69728141T2 DE69728141T DE69728141T DE69728141T2 DE 69728141 T2 DE69728141 T2 DE 69728141T2 DE 69728141 T DE69728141 T DE 69728141T DE 69728141 T DE69728141 T DE 69728141T DE 69728141 T2 DE69728141 T2 DE 69728141T2
- Authority
- DE
- Germany
- Prior art keywords
- gel
- polysaccharide
- steps
- solution
- crosslinking agent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 79
- 238000004587 chromatography analysis Methods 0.000 title claims description 4
- 238000004519 manufacturing process Methods 0.000 title abstract description 6
- 150000004676 glycans Chemical class 0.000 claims abstract description 78
- 239000005017 polysaccharide Substances 0.000 claims abstract description 78
- 238000000034 method Methods 0.000 claims abstract description 53
- 239000003431 cross linking reagent Substances 0.000 claims abstract description 35
- 238000004132 cross linking Methods 0.000 claims abstract description 24
- 230000008569 process Effects 0.000 claims abstract description 22
- 125000002887 hydroxy group Chemical group [H]O* 0.000 claims abstract description 20
- 239000006185 dispersion Substances 0.000 claims abstract description 17
- 238000007796 conventional method Methods 0.000 claims abstract 8
- 239000000499 gel Substances 0.000 claims description 118
- 239000000243 solution Substances 0.000 claims description 66
- 229920000936 Agarose Polymers 0.000 claims description 41
- 239000002245 particle Substances 0.000 claims description 40
- STMDPCBYJCIZOD-UHFFFAOYSA-N 2-(2,4-dinitroanilino)-4-methylpentanoic acid Chemical compound CC(C)CC(C(O)=O)NC1=CC=C([N+]([O-])=O)C=C1[N+]([O-])=O STMDPCBYJCIZOD-UHFFFAOYSA-N 0.000 claims description 15
- 230000006855 networking Effects 0.000 claims description 15
- -1 allyl halide Chemical class 0.000 claims description 6
- 239000007864 aqueous solution Substances 0.000 claims description 6
- 150000001875 compounds Chemical class 0.000 claims description 6
- 239000003960 organic solvent Substances 0.000 claims description 5
- AFOSIXZFDONLBT-UHFFFAOYSA-N divinyl sulfone Chemical compound C=CS(=O)(=O)C=C AFOSIXZFDONLBT-UHFFFAOYSA-N 0.000 claims description 4
- LIHMQSPBDBGMJW-UHFFFAOYSA-N 1,1-dibromopropan-1-ol Chemical compound CCC(O)(Br)Br LIHMQSPBDBGMJW-UHFFFAOYSA-N 0.000 claims description 3
- FGUUSXIOTUKUDN-IBGZPJMESA-N C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 Chemical compound C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 FGUUSXIOTUKUDN-IBGZPJMESA-N 0.000 claims description 3
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- 238000004366 reverse phase liquid chromatography Methods 0.000 claims description 2
- BHELZAPQIKSEDF-UHFFFAOYSA-N allyl bromide Chemical compound BrCC=C BHELZAPQIKSEDF-UHFFFAOYSA-N 0.000 claims 1
- 239000013522 chelant Substances 0.000 claims 1
- 230000003213 activating effect Effects 0.000 abstract description 3
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- 125000003903 2-propenyl group Chemical group [H]C([*])([H])C([H])=C([H])[H] 0.000 description 4
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- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 2
- 108010034949 Thyroglobulin Proteins 0.000 description 2
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- SLBOQBILGNEPEB-UHFFFAOYSA-N 1-chloroprop-2-enylbenzene Chemical compound C=CC(Cl)C1=CC=CC=C1 SLBOQBILGNEPEB-UHFFFAOYSA-N 0.000 description 1
- 101710134784 Agnoprotein Proteins 0.000 description 1
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- ZZSNKZQZMQGXPY-UHFFFAOYSA-N Ethyl cellulose Chemical compound CCOCC1OC(OC)C(OCC)C(OCC)C1OC1C(O)C(O)C(OC)C(CO)O1 ZZSNKZQZMQGXPY-UHFFFAOYSA-N 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 1
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- 229920000881 Modified starch Polymers 0.000 description 1
- CNCOEDDPFOAUMB-UHFFFAOYSA-N N-Methylolacrylamide Chemical compound OCNC(=O)C=C CNCOEDDPFOAUMB-UHFFFAOYSA-N 0.000 description 1
- ABLZXFCXXLZCGV-UHFFFAOYSA-N Phosphorous acid Chemical compound OP(O)=O ABLZXFCXXLZCGV-UHFFFAOYSA-N 0.000 description 1
- 229920002684 Sepharose Polymers 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
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- 125000001165 hydrophobic group Chemical group 0.000 description 1
- JGJLWPGRMCADHB-UHFFFAOYSA-N hypobromite Inorganic materials Br[O-] JGJLWPGRMCADHB-UHFFFAOYSA-N 0.000 description 1
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- 238000007639 printing Methods 0.000 description 1
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- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
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- 238000004062 sedimentation Methods 0.000 description 1
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- 229910052708 sodium Inorganic materials 0.000 description 1
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
- B01J20/291—Gel sorbents
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
- B01J20/26—Synthetic macromolecular compounds
- B01J20/262—Synthetic macromolecular compounds obtained otherwise than by reactions only involving carbon to carbon unsaturated bonds, e.g. obtained by polycondensation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
- B01J20/26—Synthetic macromolecular compounds
- B01J20/265—Synthetic macromolecular compounds modified or post-treated polymers
- B01J20/267—Cross-linked polymers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
- B01J20/282—Porous sorbents
- B01J20/285—Porous sorbents based on polymers
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0036—Galactans; Derivatives thereof
- C08B37/0039—Agar; Agarose, i.e. D-galactose, 3,6-anhydro-D-galactose, methylated, sulfated, e.g. from the red algae Gelidium and Gracilaria; Agaropectin; Derivatives thereof, e.g. Sepharose, i.e. crosslinked agarose
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/32—Bonded phase chromatography
- B01D15/325—Reversed phase
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/32—Bonded phase chromatography
- B01D15/325—Reversed phase
- B01D15/327—Reversed phase with hydrophobic interaction
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/34—Size-selective separation, e.g. size-exclusion chromatography; Gel filtration; Permeation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
- B01D15/3804—Affinity chromatography
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
- B01D15/3804—Affinity chromatography
- B01D15/3828—Ligand exchange chromatography, e.g. complexation, chelation or metal interaction chromatography
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2220/00—Aspects relating to sorbent materials
- B01J2220/50—Aspects relating to the use of sorbent or filter aid materials
- B01J2220/54—Sorbents specially adapted for analytical or investigative chromatography
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Dispersion Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE9601368 | 1996-04-11 | ||
| SE9601368A SE9601368D0 (sv) | 1996-04-11 | 1996-04-11 | Process for the production of a porous cross-linked polysaccharide gel |
| PCT/SE1997/000578 WO1997038018A1 (en) | 1996-04-11 | 1997-04-07 | Process for the production of a porous cross-linked polysaccharide gel and its use as a gel filtration media and in chromatography |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69728141D1 DE69728141D1 (de) | 2004-04-22 |
| DE69728141T2 true DE69728141T2 (de) | 2005-02-17 |
Family
ID=20402148
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69728141T Expired - Lifetime DE69728141T2 (de) | 1996-04-11 | 1997-04-07 | Verfahren zur herstellung eines porösen vernetzten polysaccharidgels und seine verwendung als filtermedien und in der chromatographie |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US6602990B1 (Direct) |
| EP (1) | EP0892815B1 (Direct) |
| JP (1) | JP4081143B2 (Direct) |
| AT (1) | ATE261992T1 (Direct) |
| AU (1) | AU726086B2 (Direct) |
| DE (1) | DE69728141T2 (Direct) |
| DK (1) | DK0892815T3 (Direct) |
| ES (1) | ES2217413T3 (Direct) |
| SE (1) | SE9601368D0 (Direct) |
| WO (1) | WO1997038018A1 (Direct) |
Families Citing this family (88)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5998606A (en) * | 1997-11-10 | 1999-12-07 | Grandics; Peter | Mn(IV)-mediated crosslinking and functionalization of chromatography media |
| DE19803415A1 (de) * | 1998-01-29 | 1999-08-05 | Aventis Res & Tech Gmbh & Co | Trennung von Stoffgemischen unter Einsatz von Polysacchariden |
| SE9901825D0 (sv) | 1999-05-20 | 1999-05-20 | Amersham Pharm Biotech Ab | Foamed material filled with inner material |
| SE0004928D0 (sv) | 2000-12-29 | 2000-12-29 | Apbiotech Ab | A method for the manufacturing of porous material |
| SE0004929D0 (sv) * | 2000-12-29 | 2000-12-29 | Apbiotech Ab | A method for producing liquid chromatography matrices |
| KR101013252B1 (ko) * | 2003-03-26 | 2011-02-09 | 나고야 인더스트리얼 사이언스 리서치 인스티튜트 | 크로마토그래피용 분리제 및 그의 제조 방법 |
| SE0400490D0 (sv) | 2004-02-26 | 2004-02-26 | Amersham Biosciences Ab | Plasmid purification |
| SE0402322D0 (sv) * | 2004-09-22 | 2004-09-22 | Amersham Biosciences Ab | Method of preparing a chromatography matrix |
| WO2006033634A1 (en) * | 2004-09-22 | 2006-03-30 | Ge Healthcare Bio-Sciences Ab | Method of preparing a chromatography matrix |
| KR101243425B1 (ko) | 2004-10-21 | 2013-03-13 | 지이 헬스케어 바이오-사이언시스 에이비 | 항체의 정제 방법 |
| CA2586803C (en) * | 2004-12-14 | 2012-12-11 | Ge Healthcare Bio-Sciences Ab | Purification of immunoglobulins |
| US8728828B2 (en) * | 2004-12-22 | 2014-05-20 | Ge Healthcare Bio-Sciences Ab | Purification of immunoglobulins |
| WO2006098671A1 (en) * | 2005-02-14 | 2006-09-21 | Ge Healthcare Bio-Sciences Ab | Liquid chromatography method |
| AU2006266529B2 (en) * | 2005-07-06 | 2010-12-16 | Cytiva Bioprocess R&D Ab | Method of preparing a separation matrix |
| SE529259C2 (sv) * | 2005-08-31 | 2007-06-12 | Ge Healthcare Bio Sciences Ab | Tillverkning av kromarografimatriser, en kromarografimatris, en vätskekromatografikolonn, prcess för att isolera målföreningar och användning av en kromarografimatris för vätskekromatografi |
| SG131016A1 (en) * | 2005-09-19 | 2007-04-26 | Millipore Corp | Asymmetric porous adsorptive bead |
| WO2007058599A1 (en) * | 2005-11-21 | 2007-05-24 | Ge Healthcare Bio-Sciences Ab | Manufacture of a chromatography matrix |
| US20070161785A1 (en) * | 2005-12-05 | 2007-07-12 | Clontech Laboratories, Inc. | High density metal ion affinity compositions and methods for making and using the same |
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| WO2025083267A1 (en) | 2023-10-20 | 2025-04-24 | Cytiva Bioprocess R&D Ab | Aav9 binding polypeptides |
| EP4541814A1 (en) | 2023-10-20 | 2025-04-23 | Cytiva BioProcess R&D AB | Stabilized polypeptides |
| EP4541444A1 (en) | 2023-10-20 | 2025-04-23 | Cytiva BioProcess R&D AB | Fusion protein for affinity capture |
| WO2025186218A1 (en) | 2024-03-04 | 2025-09-12 | Cytiva Bioprocess R&D Ab | Purification and analysis of rna |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3860573A (en) * | 1971-10-01 | 1975-01-14 | Orion Yhtymae Oy | Method for cross-linking agarose or agar |
| US3873514A (en) | 1974-05-17 | 1975-03-25 | Bio Rad Laboratories | Preparation of gel for affinity chromatography |
| SE449225B (sv) * | 1980-12-18 | 1987-04-13 | Gelinnovation Hb | Gelprodukt baserad pa partiklar innehallande galaktan for separationsendamal, molekyl- och partikelimmobilisering |
| SE8205908D0 (sv) * | 1982-10-18 | 1982-10-18 | Pharmacia Diagnostics Ab | Sett att bilda polymerskikt |
| SE437270B (sv) * | 1983-07-19 | 1985-02-18 | Pharmacia Ab | Separationsmaterial av tverbunden agaros och dess framstellning separationsmaterial av tverbunden agaros och dess framstellning |
| US4582865A (en) * | 1984-12-06 | 1986-04-15 | Biomatrix, Inc. | Cross-linked gels of hyaluronic acid and products containing such gels |
| SE458525B (sv) * | 1985-05-23 | 1989-04-10 | Pharmacia Ab | Foerfarande foer tvaerbindning av en poroes agar- eller agarosgel |
| CA1329800C (en) * | 1987-12-29 | 1994-05-24 | Hiroaki Takayanagi | Composite separating agent |
| JPH07503943A (ja) * | 1991-10-29 | 1995-04-27 | クローバー コンソリデイテッド，リミテッド | 封入及び薬剤放出に有用な架橋性の多糖類、ポリカチオン及び脂質 |
| SE9200827D0 (sv) * | 1992-03-18 | 1992-03-18 | Pharmacia Lkb Biotech | Supet porous polysaccharide gels |
| US5371208A (en) * | 1992-12-30 | 1994-12-06 | Guest Elchrom Scientific Ltd. | Preparation of cross-linked linear polysaccharide polymers as gels for electrophoresis |
| US5785832A (en) * | 1995-07-26 | 1998-07-28 | Chiari; Marcella | Covalently cross-linked, mixed-bed agarose-polyacrylamide matrices for electrophoresis and chromatography |
| US5840877A (en) * | 1996-09-05 | 1998-11-24 | Guest Elchrom Scientific | Electrophoresis gels of enhanced selectivity |
| SE9700769D0 (sv) * | 1997-03-04 | 1997-03-04 | Pharmacia Biotech Ab | Matriser för separation och separation som utnyttjar matriserna |
-
1996
- 1996-04-11 SE SE9601368A patent/SE9601368D0/xx unknown
-
1997
- 1997-04-07 EP EP97919836A patent/EP0892815B1/en not_active Expired - Lifetime
- 1997-04-07 ES ES97919836T patent/ES2217413T3/es not_active Expired - Lifetime
- 1997-04-07 AT AT97919836T patent/ATE261992T1/de active
- 1997-04-07 WO PCT/SE1997/000578 patent/WO1997038018A1/en not_active Ceased
- 1997-04-07 US US09/155,995 patent/US6602990B1/en not_active Expired - Lifetime
- 1997-04-07 DK DK97919836T patent/DK0892815T3/da active
- 1997-04-07 JP JP53612197A patent/JP4081143B2/ja not_active Expired - Fee Related
- 1997-04-07 DE DE69728141T patent/DE69728141T2/de not_active Expired - Lifetime
- 1997-04-07 AU AU24178/97A patent/AU726086B2/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| EP0892815A1 (en) | 1999-01-27 |
| JP4081143B2 (ja) | 2008-04-23 |
| AU726086B2 (en) | 2000-11-02 |
| AU2417897A (en) | 1997-10-29 |
| WO1997038018A1 (en) | 1997-10-16 |
| DK0892815T3 (da) | 2004-06-21 |
| SE9601368D0 (sv) | 1996-04-11 |
| ES2217413T3 (es) | 2004-11-01 |
| US6602990B1 (en) | 2003-08-05 |
| EP0892815B1 (en) | 2004-03-17 |
| DE69728141D1 (de) | 2004-04-22 |
| ATE261992T1 (de) | 2004-04-15 |
| JP2000508361A (ja) | 2000-07-04 |
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