DE69637001T2 - Elektrochemilumineszenz-enzymimmunoassay - Google Patents

Elektrochemilumineszenz-enzymimmunoassay Download PDF

Info

Publication number
DE69637001T2
DE69637001T2 DE69637001T DE69637001T DE69637001T2 DE 69637001 T2 DE69637001 T2 DE 69637001T2 DE 69637001 T DE69637001 T DE 69637001T DE 69637001 T DE69637001 T DE 69637001T DE 69637001 T2 DE69637001 T2 DE 69637001T2
Authority
DE
Germany
Prior art keywords
electrochemiluminescent
ecl
electrochemiluminescence
substrate
enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69637001T
Other languages
German (de)
English (en)
Other versions
DE69637001D1 (de
Inventor
Mark T. North Bethesda MARTIN
Richard Gaithersburg SAUL
Pam Arlington LIANG
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bioveris Corp
Original Assignee
Bioveris Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bioveris Corp filed Critical Bioveris Corp
Application granted granted Critical
Publication of DE69637001D1 publication Critical patent/DE69637001D1/de
Publication of DE69637001T2 publication Critical patent/DE69637001T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531Production of immunochemical test materials
    • G01N33/532Production of labelled immunochemicals
    • G01N33/535Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07FACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F15/00Compounds containing elements of Groups 8, 9, 10 or 18 of the Periodic Table
    • C07F15/0006Compounds containing elements of Groups 8, 9, 10 or 18 of the Periodic Table compounds of the platinum group
    • C07F15/0046Ruthenium compounds
    • C07F15/0053Ruthenium compounds without a metal-carbon linkage
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/44Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere, e.g. haptens, metals, DNA, RNA, amino acids
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531Production of immunochemical test materials
    • G01N33/532Production of labelled immunochemicals
    • G01N33/533Production of labelled immunochemicals with fluorescent label

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE69637001T 1995-06-07 1996-06-06 Elektrochemilumineszenz-enzymimmunoassay Expired - Fee Related DE69637001T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US48476695A 1995-06-07 1995-06-07
US484766 1995-06-07
PCT/US1996/010119 WO1996041175A1 (en) 1995-06-07 1996-06-06 Electrochemiluminescent enzyme immunoassay

Publications (2)

Publication Number Publication Date
DE69637001D1 DE69637001D1 (de) 2007-05-10
DE69637001T2 true DE69637001T2 (de) 2007-12-06

Family

ID=23925520

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69637001T Expired - Fee Related DE69637001T2 (de) 1995-06-07 1996-06-06 Elektrochemilumineszenz-enzymimmunoassay

Country Status (9)

Country Link
US (2) US6524865B1 (https=)
EP (1) EP0871887B1 (https=)
JP (1) JP3784072B2 (https=)
AT (1) ATE358276T1 (https=)
AU (1) AU718767B2 (https=)
CA (1) CA2223027A1 (https=)
DE (1) DE69637001T2 (https=)
ES (1) ES2285713T3 (https=)
WO (1) WO1996041175A1 (https=)

Families Citing this family (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5866434A (en) * 1994-12-08 1999-02-02 Meso Scale Technology Graphitic nanotubes in luminescence assays
US5641623A (en) * 1995-01-04 1997-06-24 Martin; Mark T. Electrochemiluminescence assay
US6319670B1 (en) * 1995-05-09 2001-11-20 Meso Scale Technology Llp Methods and apparatus for improved luminescence assays using microparticles
AU729118B2 (en) * 1997-02-06 2001-01-25 University Of North Carolina At Chapel Hill, The Electrochemical probes for detection of molecular interactions and drug discovery
AU6115300A (en) * 1999-07-19 2001-02-05 California Institute Of Technology Detection of biomolecules by sensitizer-linked substrates
CN101057145B (zh) * 2004-06-23 2014-10-22 得克萨斯系统大学评议会 使用双粒子复合体检测生物分子的方法和组合物
US7906276B2 (en) 2004-06-30 2011-03-15 Kimberly-Clark Worldwide, Inc. Enzymatic detection techniques
US7521226B2 (en) 2004-06-30 2009-04-21 Kimberly-Clark Worldwide, Inc. One-step enzymatic and amine detection technique
US7094528B2 (en) * 2004-06-30 2006-08-22 Kimberly-Clark Worldwide, Inc. Magnetic enzyme detection techniques
US20080261243A1 (en) * 2004-10-06 2008-10-23 Wellstat Biologics Corporation Detection of Elevated Levels of Her-2/Neu Protein on Circulating Cancer Cells and Treatment
US20090145485A1 (en) * 2005-08-11 2009-06-11 Eksigent Technologies, Llc Microfluidic methods and apparatuses for fluid mixing and valving
KR20080072748A (ko) 2005-11-29 2008-08-06 가미다-셀 리미티드 줄기세포 귀소 및 생착을 향상시키는 방법
US7820394B2 (en) * 2005-12-02 2010-10-26 Catassays Ultrasensitive bioanalytical assays based on the use of high-gain catalytic chemical amplification
JP5025724B2 (ja) * 2006-04-18 2012-09-12 ウェルスタット バイオロジクス コーポレイション 循環腫瘍性細胞からのタンパク質の検出
CA2684265A1 (en) * 2007-04-19 2008-10-30 Wellstat Biologics Corporation Detection of elevated levels of her-2/neu protein from non-isolated circulating cancer cells and treatment
JP4887319B2 (ja) 2008-03-17 2012-02-29 株式会社日立ハイテクノロジーズ 自動分析装置、及び光電子増倍管を用いた分析システム
PT2271938E (pt) 2008-04-11 2014-05-09 Univ Texas Método e aparelho de amplificação de quimiluminescênci electrogerada por nanopartículas
AU2009244904B2 (en) 2008-05-08 2014-04-17 Board Of Regents Of The University Of Texas System Luminescent nanostructured materials for use in electrogenerated chemiluminescence
WO2009136613A1 (ja) 2008-05-08 2009-11-12 株式会社 日立ハイテクノロジーズ 自動分析装置
JP5124498B2 (ja) 2009-01-30 2013-01-23 株式会社日立ハイテクノロジーズ 自動分析装置
WO2012093704A1 (ja) * 2011-01-07 2012-07-12 中外製薬株式会社 抗体の物性を改善させる方法
WO2013053953A1 (en) * 2011-10-14 2013-04-18 Université de Liège Method for measuring beta-lactam antibiotics
US9625465B2 (en) 2012-05-15 2017-04-18 Defined Diagnostics, Llc Clinical diagnostic systems
US9213043B2 (en) 2012-05-15 2015-12-15 Wellstat Diagnostics, Llc Clinical diagnostic system including instrument and cartridge
US9075042B2 (en) 2012-05-15 2015-07-07 Wellstat Diagnostics, Llc Diagnostic systems and cartridges
AU2013353573B2 (en) 2012-12-06 2019-01-17 Enlivex Therapeutics R&D Ltd Therapeutic apoptotic cell preparations, method for producing same and uses thereof
EP3258269B1 (en) * 2015-02-10 2019-06-05 Shenzhen New Industries Biomedical Engineering Co. Ltd. Reagent kit used for detecting lipoprotein-associated phospholipase a2, and preparation method and application for reagent kit
JP6979941B2 (ja) 2015-08-20 2021-12-15 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft Peg化分析物特異的結合剤を用いた粒子に基づくイムノアッセイ
BR112018017336A2 (pt) 2016-03-07 2018-12-26 Hoffmann La Roche método in vitro para detectar um anticorpo para p53 (anticorpo anti-p53) em uma amostra e polipeptídeo de fusão
JP7068323B2 (ja) 2017-02-02 2022-05-16 エフ.ホフマン-ラ ロシュ アーゲー 少なくとも2種のペグ化された分析物特異的結合剤を使用する免疫アッセイ
CN106645111A (zh) * 2017-03-01 2017-05-10 桂林理工大学 一种电化学发光快速检测抗生素ZwittermicinA浓度的方法
EP3781600A1 (en) 2018-04-18 2021-02-24 F. Hoffmann-La Roche AG Novel anti-thymidine kinase antibodies
JP7577647B2 (ja) 2018-08-31 2024-11-05 エフ. ホフマン-ラ ロシュ アーゲー Dlbclについての予後指標におけるチミジンキナーゼ(tk-1)
WO2020146497A1 (en) * 2019-01-09 2020-07-16 The Regents Of The University Of California An electrochemiluminescence elisa for ps129-a-synuclein
CN109839501B (zh) * 2019-01-30 2022-04-08 东南大学 一种测定循环肿瘤细胞的电致化学发光免疫传感器及其制备方法与应用
EP4004551A1 (en) 2019-07-22 2022-06-01 F. Hoffmann-La Roche AG Substance p as blood biomarker for the non-invasive diagnosis of endometriosis
EP4004553A1 (en) 2019-07-22 2022-06-01 F. Hoffmann-La Roche AG S100a8 as blood biomarker for the non-invasive diagnosis of endometriosis
EP4004555A1 (en) 2019-07-22 2022-06-01 F. Hoffmann-La Roche AG S100a6 as blood biomarker for the non-invasive diagnosis of endometriosis
WO2021013785A1 (en) 2019-07-22 2021-01-28 F. Hoffmann-La Roche Ag S100a9 as blood biomarker for the non-invasive diagnosis of endometriosis
WO2021013783A1 (en) 2019-07-22 2021-01-28 F. Hoffmann-La Roche Ag S100a12 as blood biomarker for the non-invasive diagnosis of endometriosis
KR20220100883A (ko) 2019-11-15 2022-07-18 에프. 호프만-라 로슈 아게 환자 샘플의 질량분석법 측정을 위한 베타-락탐 항생제의 유도체화
JP7851928B2 (ja) 2020-11-02 2026-04-27 エフ. ホフマン-ラ ロシュ アーゲー SARS-CoV-2ヌクレオカプシド抗体
CN112964768A (zh) * 2021-03-23 2021-06-15 海南微氪生物科技股份有限公司 一种Bst DNA聚合酶电致化学发光测定方法
WO2022207628A1 (en) 2021-03-30 2022-10-06 F. Hoffmann-La Roche Ag Scf as blood biomarker for the non-invasive diagnosis of endometriosis
WO2022207685A1 (en) 2021-04-01 2022-10-06 F. Hoffmann-La Roche Ag Psp94 as blood biomarker for the non-invasive diagnosis of endometriosis
CN117337394A (zh) 2021-05-17 2024-01-02 豪夫迈·罗氏有限公司 sFRP4作为血液生物标志物用于子宫腺肌病的非侵入性诊断
WO2023072904A1 (en) 2021-10-26 2023-05-04 F. Hoffmann-La Roche Ag Monoclonal antibodies specific for sars-cov-2 rbd
WO2023131594A1 (en) 2022-01-05 2023-07-13 F. Hoffmann-La Roche Ag Derivatization of compounds in patient samples for therapeutic drug monitoring (tdm)
JP2025520600A (ja) 2022-06-23 2025-07-03 エフ. ホフマン-ラ ロシュ アーゲー 子宮内膜症を診断するためおよび子宮内膜症の病期を分類するための方法
JP2025524027A (ja) 2022-07-22 2025-07-25 エフ. ホフマン-ラ ロシュ アーゲー 多嚢胞性卵巣症候群の診断のための(血液)バイオマーカーとしてのメテオリン様タンパク質(metrnl)
EP4558830A1 (en) 2022-07-22 2025-05-28 F. Hoffmann-La Roche AG Leukotriene a4 hydrolase (lta4h) as (blood) biomarker for the diagnosis of polycystic ovarian syndrome
EP4558826A1 (en) 2022-07-22 2025-05-28 F. Hoffmann-La Roche AG Fibroblast growth factor binding protein 1 (fgfbp1) as (blood) biomarker for the diagnosis of polycystic ovarian syndrome
CN121532654A (zh) 2023-07-20 2026-02-13 豪夫迈·罗氏有限公司 针对抗体组合物的筛选方法
CN121752901A (zh) 2023-07-28 2026-03-27 豪夫迈·罗氏有限公司 作为子宫内膜异位症的生物标志物的血清EphA1

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4293310A (en) * 1980-03-14 1981-10-06 University Of Pittsburgh Photoelectrochemical immunoassay
US4818709A (en) * 1983-01-21 1989-04-04 Primus Frederick J CEA-family antigens, Anti-CEA antibodies and CEA immunoassay
GB8305324D0 (en) 1983-02-25 1983-03-30 Unilever Plc Microbiological test processes
US4470459A (en) 1983-05-09 1984-09-11 Halliburton Company Apparatus and method for controlled temperature heating of volumes of hydrocarbonaceous materials in earth formations
US5310687A (en) * 1984-10-31 1994-05-10 Igen, Inc. Luminescent metal chelate labels and means for detection
US5238808A (en) 1984-10-31 1993-08-24 Igen, Inc. Luminescent metal chelate labels and means for detection
US5221605A (en) * 1984-10-31 1993-06-22 Igen, Inc. Luminescent metal chelate labels and means for detection
US4877725A (en) * 1985-04-01 1989-10-31 New York Blood Center, Inc. Immunoassays for antibodies which bind to the acquired immunodeficiency virus
JPH0737464B2 (ja) * 1986-04-30 1995-04-26 イゲン,インコーポレーテッド エレクトロ化学ルミネセンスアツセイ
US5147806A (en) 1988-04-29 1992-09-15 Igen, Inc. Method and apparatus for conducting electrochemiluminescence measurements
US4931223A (en) * 1986-07-24 1990-06-05 Tropix, Inc. Methods of using chemiluminescent 1,2-dioxetanes
WO1989004302A1 (en) * 1987-11-04 1989-05-18 Igen, Inc. Electrochemiluminescent rhenium moieties and methods
US5296191A (en) 1988-11-03 1994-03-22 Igen, Inc. Method and apparatus for conducting electrochemiluminescent measurements
US5061445A (en) 1988-11-03 1991-10-29 Igen, Inc. Apparatus for conducting measurements of electrochemiluminescent phenomena
US5247243A (en) 1988-11-03 1993-09-21 Igen, Inc. Method and apparatus for conducting electrochemiluminescent measurements
US5068808A (en) 1988-11-22 1991-11-26 Reality Imaging Corporation Imager and process
US4978613A (en) * 1989-01-17 1990-12-18 Abbott Laboratories Beta-lactamase assay employing chromogenic precipitating substrates
US5187068A (en) * 1989-06-09 1993-02-16 Nicolae Luca Method for determination of lipid moiety and apolipoprotein expressed epitope immunoreactivity on intact lipoprotein
US5264346A (en) 1991-06-03 1993-11-23 Chen Kirk C S Colorimetric method for beta-lactamase assay and its applications
US6030773A (en) * 1992-07-08 2000-02-29 Agnello; Vincent Chemiluminescent assay for DSDNA antibodies
US5527710A (en) * 1994-12-02 1996-06-18 Igen, Inc. Rate measurements of biomolecular reactions using electrochemiluminescence
US5641623A (en) * 1995-01-04 1997-06-24 Martin; Mark T. Electrochemiluminescence assay
FR2729759A1 (fr) * 1995-01-19 1996-07-26 Pasteur Sanofi Diagnostics Procede de dosage ultrasensible de la troponine i cardiaque

Also Published As

Publication number Publication date
WO1996041175A1 (en) 1996-12-19
US20040096918A1 (en) 2004-05-20
US6524865B1 (en) 2003-02-25
JPH11507726A (ja) 1999-07-06
EP0871887A1 (en) 1998-10-21
AU6384496A (en) 1996-12-30
ES2285713T3 (es) 2007-11-16
EP0871887B1 (en) 2007-03-28
JP3784072B2 (ja) 2006-06-07
US7018802B2 (en) 2006-03-28
AU718767B2 (en) 2000-04-20
DE69637001D1 (de) 2007-05-10
CA2223027A1 (en) 1996-12-19
ATE358276T1 (de) 2007-04-15
EP0871887A4 (en) 2000-04-26

Similar Documents

Publication Publication Date Title
DE69637001T2 (de) Elektrochemilumineszenz-enzymimmunoassay
EP0809111B1 (de) Bestimmungsverfahren mit oligomerisierten Rezeptoren
DE69214942T2 (de) Verfahren für spezifische bindungsassays unter verwendung eines freisetzbaren liganden
DE69526747T2 (de) Elektrobiochemisches Verfahren, System und Elektroden zur Bestimmung eines Mitgliedes eines Bindungspaares
EP1239049B1 (de) Wasserstoffperoxid-Bestimmung mittels Oxidasen und Lanthanoid-Liganden-Komplexen
US5731147A (en) Luminescent metal chelate labels and means for detection
DE69635822T2 (de) Beobachtung von verbindungen mittels elektrochemilumineszenz
DE3588243T2 (de) Nachweisverfahren durch Induktion von Elektrochemilumineszenz
DE69630688T2 (de) Elektrochemilumineszente enzymbiosensoren
EP2390664B1 (de) Verfahren zur elektrochemischen Detektion von Bindungsreaktionen
Park et al. Development of a chemiluminescent immunosensor for chloramphenicol
JPH0137693B2 (https=)
CA2248893A1 (en) Graphitic nanotubes in luminescence assays
DE2808515A1 (de) Verfahren und reagens zur durchfuehrung von bestimmungsmethoden mit biospezifischen affinitaetsreaktionen
EP0697021B1 (de) Photoaktivierbare biotinderivate und deren einsatz zum entstören von immunoassays
DE69718684T2 (de) Tests und sonden mit enzym-markierungen
DE60222091T2 (de) Dendritische chemilumineszierende Substrate
US20120070908A1 (en) Immuno conjugate and process for preparation thereof
EP0617107B1 (en) Method of making acridinium derivative luminesce and method of detecting test material therewith
DE69528311T2 (de) Enzym-verknüpfte Chemilumineszenzbestimmung
JPH04507194A (ja) 化学発光組成物、化学発光法、及び分析的検定におけるその使用
DE60027002T2 (de) Chemilumineszenz redox-assay zur qualifizierung von analyten in biologischen proben
DE60116124T2 (de) Elektrochemilumineszenz von acridanzusammensetzungen
DE4344646A1 (de) Vorrichtung für einen kompetitiven Immuno-Assay zur Bestimmung von Haptenen
DE69518995T2 (de) Ein Verfahren zum Nachweis von Biomolekülen mit Hilfe eines enzymatischen Amplifizierungssystems

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee