DE69636721T2 - Screeningverfahren für enzyme und enzymkits - Google Patents

Screeningverfahren für enzyme und enzymkits Download PDF

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Publication number
DE69636721T2
DE69636721T2 DE69636721T DE69636721T DE69636721T2 DE 69636721 T2 DE69636721 T2 DE 69636721T2 DE 69636721 T DE69636721 T DE 69636721T DE 69636721 T DE69636721 T DE 69636721T DE 69636721 T2 DE69636721 T2 DE 69636721T2
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Germany
Prior art keywords
dna
screening
recombinant
enzymes
enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69636721T
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German (de)
English (en)
Other versions
DE69636721D1 (de
Inventor
M. Jay Encinitas SHORT
Barry Kennett Square MARRS
L. Jeffrey San Diego STEIN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF Enzymes LLC
Original Assignee
Verenium Corp
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Filing date
Publication date
Priority claimed from US08/503,606 external-priority patent/US6004788A/en
Application filed by Verenium Corp filed Critical Verenium Corp
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Publication of DE69636721D1 publication Critical patent/DE69636721D1/de
Publication of DE69636721T2 publication Critical patent/DE69636721T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE69636721T 1995-07-18 1996-07-17 Screeningverfahren für enzyme und enzymkits Expired - Lifetime DE69636721T2 (de)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US08/503,606 US6004788A (en) 1995-07-18 1995-07-18 Enzyme kits and libraries
US503606 1995-07-18
US56899495A 1995-12-07 1995-12-07
US568994 1995-12-07
US657409 1996-06-03
US08/657,409 US5958672A (en) 1995-07-18 1996-06-03 Protein activity screening of clones having DNA from uncultivated microorganisms
PCT/US1996/011854 WO1997004077A1 (en) 1995-07-18 1996-07-17 Screening methods for enzymes and enzyme kits

Publications (2)

Publication Number Publication Date
DE69636721D1 DE69636721D1 (de) 2007-01-04
DE69636721T2 true DE69636721T2 (de) 2008-01-24

Family

ID=27054566

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69636721T Expired - Lifetime DE69636721T2 (de) 1995-07-18 1996-07-17 Screeningverfahren für enzyme und enzymkits
DE69638168T Expired - Lifetime DE69638168D1 (de) 1995-07-18 1996-07-17 Screeningverfahren für Enzyme und Enzymkits

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE69638168T Expired - Lifetime DE69638168D1 (de) 1995-07-18 1996-07-17 Screeningverfahren für Enzyme und Enzymkits

Country Status (10)

Country Link
US (8) US5958672A (enExample)
EP (2) EP0839185B1 (enExample)
JP (1) JP2002514894A (enExample)
AT (2) ATE465246T1 (enExample)
AU (1) AU6547796A (enExample)
CA (1) CA2227342C (enExample)
DE (2) DE69636721T2 (enExample)
DK (2) DK1696025T3 (enExample)
ES (1) ES2277346T3 (enExample)
WO (1) WO1997004077A1 (enExample)

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US6677115B2 (en) 2004-01-13
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ATE465246T1 (de) 2010-05-15
EP1696025A3 (en) 2006-09-13
US20020172944A1 (en) 2002-11-21
US6849395B2 (en) 2005-02-01
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US6280926B1 (en) 2001-08-28
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US20040005587A1 (en) 2004-01-08
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US5958672A (en) 1999-09-28
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