DE69635146D1 - Differenzgelelektrophorese mit mehreren Übereinstimmungsfarbstoffen - Google Patents

Differenzgelelektrophorese mit mehreren Übereinstimmungsfarbstoffen

Info

Publication number
DE69635146D1
DE69635146D1 DE69635146T DE69635146T DE69635146D1 DE 69635146 D1 DE69635146 D1 DE 69635146D1 DE 69635146 T DE69635146 T DE 69635146T DE 69635146 T DE69635146 T DE 69635146T DE 69635146 D1 DE69635146 D1 DE 69635146D1
Authority
DE
Germany
Prior art keywords
dyes
sample
different
protein
proteins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69635146T
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English (en)
Other versions
DE69635146T2 (de
Inventor
Jonathan Minden
Alan Waggoner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Carnegie Mellon University
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Carnegie Mellon University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Carnegie Mellon University filed Critical Carnegie Mellon University
Application granted granted Critical
Publication of DE69635146D1 publication Critical patent/DE69635146D1/de
Publication of DE69635146T2 publication Critical patent/DE69635146T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/13—Labelling of peptides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/52—Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/80—Fluorescent dyes, e.g. rhodamine

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Electrochemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Peptides Or Proteins (AREA)
  • Cosmetics (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
DE69635146T 1995-04-20 1996-04-19 Differenzgelelektrophorese mit mehreren Übereinstimmungsfarbstoffen Expired - Lifetime DE69635146T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/425,480 US6127134A (en) 1995-04-20 1995-04-20 Difference gel electrophoresis using matched multiple dyes
US425480 1995-04-20

Publications (2)

Publication Number Publication Date
DE69635146D1 true DE69635146D1 (de) 2005-10-06
DE69635146T2 DE69635146T2 (de) 2006-06-22

Family

ID=23686744

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69634600T Expired - Lifetime DE69634600T2 (de) 1995-04-20 1996-04-19 Differenzgelelektrophorese mit mehreren übereinstmmungsfarbstoffen
DE69635146T Expired - Lifetime DE69635146T2 (de) 1995-04-20 1996-04-19 Differenzgelelektrophorese mit mehreren Übereinstimmungsfarbstoffen

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE69634600T Expired - Lifetime DE69634600T2 (de) 1995-04-20 1996-04-19 Differenzgelelektrophorese mit mehreren übereinstmmungsfarbstoffen

Country Status (9)

Country Link
US (2) US6127134A (de)
EP (2) EP0821787B1 (de)
JP (2) JP3729859B2 (de)
AT (2) ATE293252T1 (de)
AU (1) AU709733B2 (de)
CA (1) CA2218528C (de)
DE (2) DE69634600T2 (de)
ES (2) ES2240993T3 (de)
WO (1) WO1996033406A1 (de)

Families Citing this family (96)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6593148B1 (en) 1994-03-01 2003-07-15 Li-Cor, Inc. Cyanine dye compounds and labeling methods
US6426190B1 (en) * 1995-04-20 2002-07-30 Carnegie Mellon University Difference detection methods using matched multiple dyes
GB9624927D0 (en) 1996-11-29 1997-01-15 Oxford Glycosciences Uk Ltd Gels and their use
US5993627A (en) * 1997-06-24 1999-11-30 Large Scale Biology Corporation Automated system for two-dimensional electrophoresis
WO1999005221A1 (en) * 1997-07-28 1999-02-04 Nycomed Amersham Plc Cyanine dyes
US6379971B1 (en) 1998-02-24 2002-04-30 Target Discovery, Inc. Methods for sequencing proteins
GB9811656D0 (en) 1998-05-29 1998-07-29 Oxford Glycosciences Uk Ltd Gels, methods and apparatus for identification and characterization of biomolecules
US6821402B1 (en) * 1998-09-16 2004-11-23 Applera Corporation Spectral calibration of fluorescent polynucleotide separation apparatus
AU763102B2 (en) * 1998-09-16 2003-07-10 Applera Corporation Spectral calibration of fluorescent polynucleotide separation apparatus
US6716636B1 (en) 1999-04-20 2004-04-06 Target Discovery, Inc. Methods for sequencing proteins
US6764817B1 (en) 1999-04-20 2004-07-20 Target Discovery, Inc. Methods for conducting metabolic analyses
SE9903989D0 (sv) * 1999-11-03 1999-11-03 Amersham Pharm Biotech Ab Method of accurate analysis
SE9903987D0 (sv) * 1999-11-03 1999-11-03 Amersham Pharm Biotech Ab Method of comparison analysis
SE9903988D0 (sv) * 1999-11-03 1999-11-03 Amersham Pharm Biotech Ab Method of analysis
US20080096284A1 (en) * 2000-02-08 2008-04-24 Regents Of The University Of Michigan Protein separation and analysis
US20030064527A1 (en) * 2001-02-07 2003-04-03 The Regents Of The University Of Michigan Proteomic differential display
AU2001284853A1 (en) * 2000-08-11 2002-02-25 Invitrogen Corporation Highly homogeneous molecular markers for electrophoresis
EP1801165B1 (de) 2000-09-29 2012-08-01 Life Technologies Corporation Modifizierte Carbocyanin-Farbstoffe und ihre Konjugate
EP1322710B2 (de) * 2000-09-29 2015-02-18 Life Technologies Corporation Modifizierte carbocyaninfarbstoffe und deren konjugate
WO2002066952A2 (en) 2000-10-19 2002-08-29 Target Discovery, Inc Mass defect labeling for the determination of oligomer sequences
WO2002040983A1 (de) * 2000-11-16 2002-05-23 Basf Aktiengesellschaft Verfahren zur trennung und detektion von proteinen mittels elektrophorese
US7083987B2 (en) * 2001-02-05 2006-08-01 Activx Biosciences, Inc. Activity based probe analysis
WO2002071066A1 (en) * 2001-03-02 2002-09-12 Activx Biosciences, Inc. Protein profiling platform
US7045296B2 (en) 2001-05-08 2006-05-16 Applera Corporation Process for analyzing protein samples
US8323903B2 (en) 2001-10-12 2012-12-04 Life Technologies Corporation Antibody complexes and methods for immunolabeling
US20050069962A1 (en) 2001-10-12 2005-03-31 Archer Robert M Antibody complexes and methods for immunolabeling
US6972326B2 (en) 2001-12-03 2005-12-06 Molecular Probes, Inc. Labeling of immobilized proteins using dipyrrometheneboron difluoride dyes
US20030134303A1 (en) * 2001-12-11 2003-07-17 Activx Biosciences, Inc, Adenine nucleotide-binding protein-directed probes, and methods of their synthesis and use
ATE536993T1 (de) * 2002-01-02 2011-12-15 Visen Medical Inc Aminfunktionalisierte superparamagnetisierte nanoteilchen für die synthese von biokonjugaten
AU2003232089A1 (en) * 2002-05-06 2003-11-17 Perkinelmer Life Sciences, Inc. Separation process and dyes for use therewith
US7091348B2 (en) * 2002-07-01 2006-08-15 Guava Technologies, Inc. Fluorescent dyes, energy transfer couples and methods
DE60235652D1 (de) * 2002-07-08 2010-04-22 Ge Healthcare Uk Ltd Reagenzien sowie ein verfahren zur sättigungsmarkierung von proteinen
US7582260B2 (en) * 2002-07-18 2009-09-01 Montana State University Zwitterionic dyes for labeling in proteomic and other biological analyses
WO2004033397A2 (en) * 2002-10-09 2004-04-22 Activx Biosciences, Inc. Activity-based probes, and methods of their preparation and use
DE50300386D1 (de) * 2003-01-23 2005-04-28 Biovendor Lab Medicine Inc Verfahren zur Erstellung von Profilen der differentialen Proteinexpression
JP2006526137A (ja) * 2003-05-28 2006-11-16 ジーイー・ヘルスケア・ユーケイ・リミテッド 内部標準の存在下で色素を標識することによる閉じた膜構造上の細胞表面タンパク質の示差分析
US7781173B2 (en) * 2003-09-25 2010-08-24 Life Technologies Corporation Homogeneous populations of molecules
EP1934202B1 (de) 2005-09-02 2019-01-09 Visen Medical, Inc. Nah-infrarot-fluorophore, die sich von nicotinsäure und picolinsäure ableiten
WO2007028163A1 (en) * 2005-09-02 2007-03-08 Visen Medical, Inc. Biocompatible fluorescent imaging agents
AU2006284565B2 (en) 2005-09-02 2013-05-30 Visen Medical, Inc. Biocompatible N, N-disubstituted sulfonamide-containing fluorescent dye labels
WO2007034907A1 (ja) * 2005-09-22 2007-03-29 Chemicals Evaluation And Research Institute 被検化学物質の生物に与える影響の予測方法
ES2434491T3 (es) * 2005-10-13 2013-12-16 Funda��O D. Anna Sommer Champalimaud E Dr. Carlos Montez Champalimaud An�lisis inmunohistoqu�mico multiplex in situ
US9913917B2 (en) 2005-12-22 2018-03-13 Visen Medical, Inc. Biocompatible fluorescent metal oxide nanoparticles
EP1968431B2 (de) 2005-12-22 2022-06-01 Visen Medical, Inc. Kombiniertes röntgen- und optisches tomographie-bildgebungssystem
JP2009524832A (ja) 2006-01-24 2009-07-02 ライフ テクノロジーズ コーポレーション 検体を定量するためのデバイスおよび方法
US20080038761A1 (en) * 2006-01-30 2008-02-14 Invitrogen Corporation Compositions and methods for detecting and quantifying toxic substances in disease states
US8114636B2 (en) 2006-02-10 2012-02-14 Life Technologies Corporation Labeling and detection of nucleic acids
DK3438131T3 (da) 2006-02-10 2022-04-11 Life Technologies Corp Oligosaccharidmodifikation og mærkning af proteiner
US8562802B1 (en) 2006-02-13 2013-10-22 Life Technologies Corporation Transilluminator base and scanner for imaging fluorescent gels, charging devices and portable electrophoresis systems
SG171599A1 (en) 2006-03-23 2011-06-29 Absorber Ab Blood group antigens of different types for diagnostic and therapeutic applications
DK2029799T3 (da) 2006-04-20 2012-12-10 Becton Dickinson Co Termostabile proteiner og fremgangsmåder til fremstilling og anvendelse heraf
WO2008002236A1 (en) * 2006-06-28 2008-01-03 Ge Healthcare Bio-Sciences Ab Method of detecting interactions between protein components
US20080220442A1 (en) * 2006-12-06 2008-09-11 Proteinics Difference detection methods using isoelectric focusing chips
US8017104B2 (en) * 2007-02-28 2011-09-13 Carestream Health, Inc. Large stoke shift dye used for optical imaging
US8906354B2 (en) 2007-02-28 2014-12-09 Bruker Biospin Corporation Loaded latex optical molecular imaging probes containing lipophilic large stokes shift dyes
WO2008109832A2 (en) * 2007-03-08 2008-09-12 Visen Medical, Inc. Viable near-infrared fluorochrome labeled cells and methods of making and using same
GB0714202D0 (en) * 2007-07-20 2007-08-29 Ge Healthcare Bio Sciences Ab Isoelectric point markers
US8492734B2 (en) 2007-10-19 2013-07-23 Visen Medical, Inc. Imaging systems featuring waveguiding compensation
US20090142775A1 (en) * 2007-11-30 2009-06-04 Agilent Technologies, Inc. Methods For Separating Organelles
DK2244741T3 (en) 2008-01-18 2015-05-26 Visen Medical Inc Fluorescent imaging agents
CN104225613A (zh) 2008-03-14 2014-12-24 Visen医药公司 整联蛋白靶向试剂及使用其的体内和体外成像方法
EP2297344B1 (de) 2008-05-16 2018-03-14 Life Technologies Corporation Doppelmarkierungsverfahren zur messung von zellproliferation
US20110186434A1 (en) * 2008-07-23 2011-08-04 Leibniz Institute of Plant Biochemistry Qualitative and/or quantitative determination of a proteinaceous molecule in a plurality of samples
US8864821B2 (en) 2008-11-26 2014-10-21 Visen Medical, Inc. Methods and compositions for identifying subjects at risk of developing stent thrombosis
KR101149535B1 (ko) 2009-04-17 2012-05-25 한국과학기술연구원 생체 분자 표지를 위한 신규 시아닌 화합물 및 그 제조방법
DK2470887T3 (da) * 2009-08-28 2020-04-06 Visen Medical Inc Systemer til tomografisk billeddannelse i diffuse medier ved at anvende en hybrid inversionsteknik
WO2011026085A2 (en) 2009-08-31 2011-03-03 Promega Corporation Reactive cyanine compounds
WO2011038006A1 (en) 2009-09-22 2011-03-31 Visen Medical, Inc. Systems and methods for virtual index-matching of diffusive media
KR101695617B1 (ko) * 2009-09-25 2017-01-16 (주)바이오액츠 물질 표지용 벤즈인도시아닌 화합물, 그 제조 중간체 및 그 제조방법
US8974651B2 (en) 2010-04-17 2015-03-10 C.C. Imex Illuminator for visualization of fluorophores
US8623324B2 (en) 2010-07-21 2014-01-07 Aat Bioquest Inc. Luminescent dyes with a water-soluble intramolecular bridge and their biological conjugates
WO2012022734A2 (en) 2010-08-16 2012-02-23 Medimmune Limited Anti-icam-1 antibodies and methods of use
US20130208445A1 (en) * 2010-10-06 2013-08-15 Zhang-Lin Zhou Modified bodipy dye matrix
CN102095778B (zh) * 2011-01-06 2013-07-31 浙江大学 脐血来源的间充质干细胞表面差异膜蛋白的检测方法
CN102095776B (zh) * 2011-01-06 2013-07-31 浙江大学 脐带来源间充质干细胞表面差异膜蛋白的检测方法
EP2707102B1 (de) 2011-05-09 2019-11-13 Visen Medical, Inc. Auf carboanhydrase zielende wirkstoffe sowie verfahren zu ihrer verwendung
EP2831264B1 (de) 2012-03-30 2019-07-10 VisEn Medical, Inc. Bakterielle bildgebungsmittel und verfahren zur verwendung davon
JP6370785B2 (ja) 2012-08-15 2018-08-08 ビセン メディカル， インコーポレイテッド 前立腺がんイメージングのための前立腺特異的抗原薬剤およびその使用方法
CN104853669B (zh) 2012-10-15 2017-07-21 文森医学公司 具有荧光及生物发光源的交叉形态加权特征的漫射介质成像的系统、方法及设备
US20150246146A1 (en) 2012-10-25 2015-09-03 Life Technologies Corporation Methods and compositions for enzyme-mediated site-specific radiolabeling of glycoproteins
EP3000088A4 (de) 2013-05-23 2018-06-27 Iphenotype LLC Verfahren und system zur bewahrung oder verbesserung des wohlbefindens
WO2015050959A1 (en) 2013-10-01 2015-04-09 Yale University Anti-kit antibodies and methods of use thereof
AU2014329609B2 (en) 2013-10-02 2019-09-12 Humabs Biomed Sa Neutralizing anti-influenza A antibodies and uses thereof
US9636034B2 (en) 2013-10-23 2017-05-02 Verily Life Sciences Llc Non-invasive analyte detection system with modulation source
US10542918B2 (en) 2013-10-23 2020-01-28 Verily Life Sciences Llc Modulation of a response signal to distinguish between analyte and background signals
CN113349707A (zh) 2013-12-31 2021-09-07 纪念斯隆-凯特琳癌症中心 用于荧光源实时多通道成像的系统、方法和设备
US9835587B2 (en) 2014-04-01 2017-12-05 C.C. Imex Electrophoresis running tank assembly
CN107249647A (zh) 2014-12-15 2017-10-13 纪念斯隆-凯特琳癌症中心 具有增强的神经结合选择性的环状肽、与所述环状肽结合的纳米颗粒和此二者用于实时体内神经组织成像的用途
US9861710B1 (en) 2015-01-16 2018-01-09 Verily Life Sciences Llc Composite particles, methods, and in vivo diagnostic system
US10028659B2 (en) 2015-03-26 2018-07-24 Verily Life Sciences Llc Aptamer-based sensors, implantable devices and detection system
KR20180093961A (ko) 2015-12-15 2018-08-22 메모리얼 슬로안-케터링 캔서 센터 조직 구별, 예를 들어, 수술 중 시각화를 위한 영상화 시스템 및 방법
EP3443126B1 (de) 2016-04-15 2023-11-15 Icahn School of Medicine at Mount Sinai Gewebeprofilierung unter verwendung von multiplexierten immunhistochemischen aufeinanderfolgenden färbungen
EP3548096A1 (de) 2016-11-30 2019-10-09 Memorial Sloan Kettering Cancer Center Inhibitorfunktionalisierte ultrakleine nanopartikel und verfahren dafür
US20210108253A1 (en) 2017-03-30 2021-04-15 Life Technologies Corporation Quantification of ngs dna by adapter sequence
KR20230034333A (ko) 2020-07-02 2023-03-09 라이프 테크놀로지스 코포레이션 트리뉴클레오티드 캡 유사체, 제조 및 이의 용도
EP4015004A1 (de) 2020-12-18 2022-06-22 Phi Pharma SA Proteoglykanspezifische verzweigte peptide

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8513538D0 (en) * 1985-05-29 1985-07-03 Mackay C D Electrophoresis
US4855225A (en) * 1986-02-07 1989-08-08 Applied Biosystems, Inc. Method of detecting electrophoretically separated oligonucleotides
US5268486A (en) * 1986-04-18 1993-12-07 Carnegie-Mellon Unversity Method for labeling and detecting materials employing arylsulfonate cyanine dyes
US5242796A (en) * 1986-07-02 1993-09-07 E. I. Du Pont De Nemours And Company Method, system and reagents for DNA sequencing
JP2527340B2 (ja) * 1986-12-15 1996-08-21 アプライド バイオシステムズ インコーポレイテッド ロ―ダミン染料の５−及び６−スクシニミジルカルボキシレ―ト異性体
US4774339A (en) * 1987-08-10 1988-09-27 Molecular Probes, Inc. Chemically reactive dipyrrometheneboron difluoride dyes
US5262545A (en) * 1989-03-09 1993-11-16 Molecular Probes, Inc. Fluorescent chloramphenicol derivatives for determination of chloramphenicol acetyltransferase activity
US5401847A (en) * 1990-03-14 1995-03-28 Regents Of The University Of California DNA complexes with dyes designed for energy transfer as fluorescent markers
US5312921A (en) * 1990-03-14 1994-05-17 Regents Of The University Of California Dyes designed for high sensitivity detection of double-stranded DNA
US5307148A (en) * 1990-04-05 1994-04-26 Hitachi, Ltd. Fluorescence detection type electrophoresis apparatus
US5274113A (en) * 1991-11-01 1993-12-28 Molecular Probes, Inc. Long wavelength chemically reactive dipyrrometheneboron difluoride dyes and conjugates
US5248782A (en) * 1990-12-18 1993-09-28 Molecular Probes, Inc. Long wavelength heteroaryl-substituted dipyrrometheneboron difluoride dyes
GB9103073D0 (en) * 1991-02-13 1991-03-27 Astromed Ltd Improvements in or relating to electrophoresis
US5338854A (en) * 1991-02-13 1994-08-16 Molecular Probes, Inc. Fluorescent fatty acids derived from dipyrrometheneboron difluoride dyes
JP2873884B2 (ja) * 1991-03-22 1999-03-24 日立ソフトウェアエンジニアリング 株式会社 多色泳動パターン読み取り装置
US5296599A (en) * 1991-09-19 1994-03-22 Millipore Corporation Activated carbamates compounds
JPH05322771A (ja) * 1992-05-15 1993-12-07 Toyobo Co Ltd 多標識系電気泳動システム
JPH05322770A (ja) * 1992-05-15 1993-12-07 Toyobo Co Ltd 多標識系電気泳動法
US5654419A (en) * 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations

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ATE303593T1 (de) 2005-09-15
EP0821787B1 (de) 2005-04-13
CA2218528C (en) 2003-06-24
DE69635146T2 (de) 2006-06-22
ES2240993T3 (es) 2005-10-16
JP3729859B2 (ja) 2005-12-21
CA2218528A1 (en) 1996-10-24
WO1996033406A1 (en) 1996-10-24
EP1494026A1 (de) 2005-01-05
DE69634600D1 (de) 2005-05-19
ATE293252T1 (de) 2005-04-15
US6127134A (en) 2000-10-03
JP2006023314A (ja) 2006-01-26
DE69634600T2 (de) 2006-01-19
US6043025A (en) 2000-03-28
EP0821787A1 (de) 1998-02-04
JP3890071B2 (ja) 2007-03-07
AU5557396A (en) 1996-11-07
EP1494026B1 (de) 2005-08-31
ES2248781T3 (es) 2006-03-16
AU709733B2 (en) 1999-09-02
JPH11505324A (ja) 1999-05-18

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