ES2131708T3 - Utilizacion de la electroforesis capilar para determinar la cantidad de concentracion de componentes de proteina y la totalidad de proteina en los fluidos. - Google Patents

Utilizacion de la electroforesis capilar para determinar la cantidad de concentracion de componentes de proteina y la totalidad de proteina en los fluidos.

Info

Publication number
ES2131708T3
ES2131708T3 ES94929210T ES94929210T ES2131708T3 ES 2131708 T3 ES2131708 T3 ES 2131708T3 ES 94929210 T ES94929210 T ES 94929210T ES 94929210 T ES94929210 T ES 94929210T ES 2131708 T3 ES2131708 T3 ES 2131708T3
Authority
ES
Spain
Prior art keywords
protein
concentration
internal standard
sample
standard compound
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94929210T
Other languages
English (en)
Inventor
Hann-Ping Wang
Cheng-Ming Liu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Beckman Coulter Inc
Original Assignee
Beckman Coulter Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beckman Coulter Inc filed Critical Beckman Coulter Inc
Application granted granted Critical
Publication of ES2131708T3 publication Critical patent/ES2131708T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44743—Introducing samples

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Electrochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

UN METODO PARA CUANTIFICAR PROTEINAS EN MUESTRAS COMPLEJAS QUE UTILIZA ELECTROFORESIA CAPILAR PUEDE UTILIZARSE TANTO PARA DETERMINAR LA CONCENTRACION DE UNA PROTEINA EN UNA MUESTRA COMO PARA DETERMINAR LA CONCENTRACION TOTAL DE PROTEINA EN LA MUESTRA. EN GENERAL, EL METODO PARA DETERMINAR LA CONCENTRACION DE UNA PROTEINA MARCADORA INCLUYE: (1) ADICION DE UNA CANTIDAD CONOCIDA DE UN COMPUESTO INTERNO ESTANDAR A UNA MUESTRA QUE CONTIENE AL MENOS UNA PROTEINA, EL COMPUESTO INTERNO ESTANDAR SELECCIONADO DEL GRUPO QUE CONSTA DE ACIDO BENZOICO SUSTITUIDO CON AL MENOS UN HALOGENO, PRODUCIENDO UNA SEÑAL DETECTORA EN RELACION A SU CONCENTRACION, Y SIENDO CAPAZ DE LA SEPARACION ELECTROFORETICA DE LA PROTEINA; (2) SOMETIMIENTO DE LA MUESTRA Y DEL COMPUESTO INTERNO ESTANDAR A ELECTROFORESIA CAPILAR PARA SEPARAR ENTRE SI LA PROTEINA Y EL COMPUESTO INTERNO ESTANDAR Y DE OTROS COMPONENTES EN LA MUESTRA; (3) MEDICION DE LA SEÑAL DETECTORA PRODUCIDA POR EL COMPUESTO ESTANDAR INTERNO Y UNA SEÑAL DETECTORA PRODUCIDA POR LA PROTEINA PARA DETERMINAR UNA PROPORCION DE SEÑAL DE PROTEINA A LA SEÑAL INTERNA ESTANDAR; Y (4) DETERMINACION DE LA CONCENTRACION DE LA PROTEINA EN LA MUESTRA A PARTIR DE UNA CURVA ESTANDAR DE CONCENTRACION DE PROTEINA FRENTE A LA PROPORCION DE SEÑAL DE PROTEINA EN LA SEÑAL INTERNA ESTANDAR. UNA PROTEINA TIPICA QUE PUEDE MEDIRSE POR EL METODO ES ALBUMINA. NORMALMENTE, LAS MEDICIONES SE REALIZAN EN 214 NM Y EL COMPUESTO INTERNO ESTANDAR ES ACIDO 2,4-DICLOROBENZOICO.
ES94929210T 1993-10-07 1994-09-15 Utilizacion de la electroforesis capilar para determinar la cantidad de concentracion de componentes de proteina y la totalidad de proteina en los fluidos. Expired - Lifetime ES2131708T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US13351493A 1993-10-07 1993-10-07

Publications (1)

Publication Number Publication Date
ES2131708T3 true ES2131708T3 (es) 1999-08-01

Family

ID=22458964

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94929210T Expired - Lifetime ES2131708T3 (es) 1993-10-07 1994-09-15 Utilizacion de la electroforesis capilar para determinar la cantidad de concentracion de componentes de proteina y la totalidad de proteina en los fluidos.

Country Status (9)

Country Link
US (1) US5490909A (es)
EP (1) EP0672249B1 (es)
JP (1) JP3484463B2 (es)
AT (1) ATE180056T1 (es)
AU (1) AU669308B2 (es)
CA (1) CA2149903C (es)
DE (1) DE69418450T2 (es)
ES (1) ES2131708T3 (es)
WO (1) WO1995010041A1 (es)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6045995A (en) * 1994-08-24 2000-04-04 Isis Pharmaceuticals, Inc. Capillary electrophoretic detection of nucleic acids
GB9509410D0 (en) * 1995-05-10 1995-07-05 Imperial College Molecular imaging
US5543730A (en) * 1995-05-17 1996-08-06 Altera Corporation Techniques for programming programmable logic array devices
EP0852720A4 (en) * 1995-08-01 2000-02-23 Signet Diagnostic Corp METHOD FOR MEASURING THE LIQUID VOLUME AND / OR SOLIDS IN A SUSPENSION
US5922184A (en) * 1997-07-21 1999-07-13 Bio-Rad Laboratories, Inc. Computer-directed detection of paraproteins
JPH1073569A (ja) * 1996-08-30 1998-03-17 Oriental Yeast Co Ltd 蛋白の測定
US6113763A (en) 1996-11-04 2000-09-05 Board Of Trustee Operating Michigan State University Method for measuring cellular chemical profiles
US5804384A (en) * 1996-12-06 1998-09-08 Vysis, Inc. Devices and methods for detecting multiple analytes in samples
US6156179A (en) * 1998-07-09 2000-12-05 Bio-Rad Laboratories Computer directed identification of paraproteins
ITMI20011850A1 (it) * 2001-09-03 2003-03-03 Univ Pavia Metodo per la separazione e la quantificazione di due conformazioni di beta2-microglobulina in equilibrio dinamico
US7381317B2 (en) * 2002-08-12 2008-06-03 Beckman Coulter, Inc. Methods and compositions for capillary electrophoresis (CE)
US20070014699A1 (en) * 2005-06-23 2007-01-18 Beckman Coulter, Inc, Methods and apparatus for improving the sensitivity of capillary zone electrophoresis
US20100117666A1 (en) * 2006-12-28 2010-05-13 Wada Henry G Method and System for Internal Standardization of Assays
GB2460981B (en) * 2007-04-13 2011-11-09 Technologies Inc C Interactive variable pathlength device
US8337685B2 (en) * 2007-11-30 2012-12-25 Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of National Defence Serum components that bind to threat agents
DE102010047427A1 (de) * 2010-10-04 2012-04-05 Karlsruher Institut für Technologie Selektive Proteinquantifizierung mittels multivariater Auswertung von UV-Absorptionsspektren
US20220291177A1 (en) * 2019-07-23 2022-09-15 The University Of British Columbia Apparatus and methods for detecting and quantifying analytes
CN110873683B (zh) * 2019-12-04 2021-08-03 中国计量科学研究院 基于es-dma-cpc的高准确度蛋白质标准物质定值方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5917142A (ja) * 1982-07-20 1984-01-28 Olympus Optical Co Ltd デンシトグラムの記録方法
US4927265A (en) * 1988-04-29 1990-05-22 501 Microphoretic Systems, Inc. Detector for fluorescence and absorption spectroscopy
US5139630A (en) * 1991-05-31 1992-08-18 Beckman Instruments, Inc. Identification of sample constituents utilizing capillary electrophoresis
US5120413A (en) * 1991-05-31 1992-06-09 Beckman Instruments, Inc. Analysis of samples utilzing capillary electrophoresis
US5310462A (en) * 1991-05-31 1994-05-10 Beckman Instruments, Inc. Quantitation of samples utilizing capillary electrophoresis
US5145567A (en) * 1991-11-14 1992-09-08 Beckman Instruments, Inc. Capillary zone electrophoretic analysis of isoenzymes
US5202006A (en) * 1992-04-17 1993-04-13 Beckman Instruments, Inc. Analysis of hemoglobin variants by capillary zone electrophoresis

Also Published As

Publication number Publication date
EP0672249B1 (en) 1999-05-12
AU7835394A (en) 1995-05-01
EP0672249A1 (en) 1995-09-20
US5490909A (en) 1996-02-13
CA2149903C (en) 2004-03-02
WO1995010041A1 (en) 1995-04-13
JP3484463B2 (ja) 2004-01-06
AU669308B2 (en) 1996-05-30
ATE180056T1 (de) 1999-05-15
JPH08504277A (ja) 1996-05-07
DE69418450D1 (de) 1999-06-17
DE69418450T2 (de) 1999-09-16
CA2149903A1 (en) 1995-04-13

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