DE69418450D1 - Verwendung von kapillarer elektroforese zur quantitativen bestimmung von proteinkomponenten und des gesamten proteingehaltes in flussigkeiten - Google Patents

Verwendung von kapillarer elektroforese zur quantitativen bestimmung von proteinkomponenten und des gesamten proteingehaltes in flussigkeiten

Info

Publication number
DE69418450D1
DE69418450D1 DE69418450T DE69418450T DE69418450D1 DE 69418450 D1 DE69418450 D1 DE 69418450D1 DE 69418450 T DE69418450 T DE 69418450T DE 69418450 T DE69418450 T DE 69418450T DE 69418450 D1 DE69418450 D1 DE 69418450D1
Authority
DE
Germany
Prior art keywords
protein
internal standard
sample
concentration
standard compound
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69418450T
Other languages
English (en)
Other versions
DE69418450T2 (de
Inventor
Hann-Ping Wang
Cheng-Ming Liu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Beckman Coulter Inc
Original Assignee
Beckman Coulter Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beckman Coulter Inc filed Critical Beckman Coulter Inc
Publication of DE69418450D1 publication Critical patent/DE69418450D1/de
Application granted granted Critical
Publication of DE69418450T2 publication Critical patent/DE69418450T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44743—Introducing samples

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Peptides Or Proteins (AREA)
DE69418450T 1993-10-07 1994-09-15 Verwendung von kapillarer elektroforese zur quantitativen bestimmung von proteinkomponenten und des gesamten proteingehaltes in flussigkeiten Expired - Fee Related DE69418450T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13351493A 1993-10-07 1993-10-07
PCT/US1994/010402 WO1995010041A1 (en) 1993-10-07 1994-09-15 Use of capillary electrophoresis for quantitating the concentration of protein components and of the total protein in fluids

Publications (2)

Publication Number Publication Date
DE69418450D1 true DE69418450D1 (de) 1999-06-17
DE69418450T2 DE69418450T2 (de) 1999-09-16

Family

ID=22458964

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69418450T Expired - Fee Related DE69418450T2 (de) 1993-10-07 1994-09-15 Verwendung von kapillarer elektroforese zur quantitativen bestimmung von proteinkomponenten und des gesamten proteingehaltes in flussigkeiten

Country Status (9)

Country Link
US (1) US5490909A (de)
EP (1) EP0672249B1 (de)
JP (1) JP3484463B2 (de)
AT (1) ATE180056T1 (de)
AU (1) AU669308B2 (de)
CA (1) CA2149903C (de)
DE (1) DE69418450T2 (de)
ES (1) ES2131708T3 (de)
WO (1) WO1995010041A1 (de)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6045995A (en) * 1994-08-24 2000-04-04 Isis Pharmaceuticals, Inc. Capillary electrophoretic detection of nucleic acids
GB9509410D0 (en) * 1995-05-10 1995-07-05 Imperial College Molecular imaging
US5543730A (en) * 1995-05-17 1996-08-06 Altera Corporation Techniques for programming programmable logic array devices
CA2228473C (en) * 1995-08-01 2007-01-30 Signet Diagnostic Corporation Method for measuring the volume of liquid and/or solid in a suspension
US5922184A (en) * 1997-07-21 1999-07-13 Bio-Rad Laboratories, Inc. Computer-directed detection of paraproteins
JPH1073569A (ja) * 1996-08-30 1998-03-17 Oriental Yeast Co Ltd 蛋白の測定
US6113763A (en) * 1996-11-04 2000-09-05 Board Of Trustee Operating Michigan State University Method for measuring cellular chemical profiles
US5804384A (en) * 1996-12-06 1998-09-08 Vysis, Inc. Devices and methods for detecting multiple analytes in samples
US6156179A (en) * 1998-07-09 2000-12-05 Bio-Rad Laboratories Computer directed identification of paraproteins
ITMI20011850A1 (it) * 2001-09-03 2003-03-03 Univ Pavia Metodo per la separazione e la quantificazione di due conformazioni di beta2-microglobulina in equilibrio dinamico
US7381317B2 (en) 2002-08-12 2008-06-03 Beckman Coulter, Inc. Methods and compositions for capillary electrophoresis (CE)
US20070014699A1 (en) * 2005-06-23 2007-01-18 Beckman Coulter, Inc, Methods and apparatus for improving the sensitivity of capillary zone electrophoresis
ES2388005T3 (es) * 2006-12-28 2012-10-05 Wako Pure Chemical Industries, Ltd. Procedimiento de normalización interna de ensayos
GB2460981B (en) * 2007-04-13 2011-11-09 Technologies Inc C Interactive variable pathlength device
CA2646944A1 (en) * 2007-11-30 2009-05-30 Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of National Defence Serum components that bind to threat agents
DE102010047427A1 (de) * 2010-10-04 2012-04-05 Karlsruher Institut für Technologie Selektive Proteinquantifizierung mittels multivariater Auswertung von UV-Absorptionsspektren
WO2021012053A1 (en) * 2019-07-23 2021-01-28 The University Of British Columbia Apparatus and methods for detecting and quantifying analytes
CN110873683B (zh) * 2019-12-04 2021-08-03 中国计量科学研究院 基于es-dma-cpc的高准确度蛋白质标准物质定值方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5917142A (ja) * 1982-07-20 1984-01-28 Olympus Optical Co Ltd デンシトグラムの記録方法
US4927265A (en) * 1988-04-29 1990-05-22 501 Microphoretic Systems, Inc. Detector for fluorescence and absorption spectroscopy
US5139630A (en) * 1991-05-31 1992-08-18 Beckman Instruments, Inc. Identification of sample constituents utilizing capillary electrophoresis
US5310462A (en) * 1991-05-31 1994-05-10 Beckman Instruments, Inc. Quantitation of samples utilizing capillary electrophoresis
US5120413A (en) * 1991-05-31 1992-06-09 Beckman Instruments, Inc. Analysis of samples utilzing capillary electrophoresis
US5145567A (en) * 1991-11-14 1992-09-08 Beckman Instruments, Inc. Capillary zone electrophoretic analysis of isoenzymes
US5202006A (en) * 1992-04-17 1993-04-13 Beckman Instruments, Inc. Analysis of hemoglobin variants by capillary zone electrophoresis

Also Published As

Publication number Publication date
JPH08504277A (ja) 1996-05-07
AU669308B2 (en) 1996-05-30
JP3484463B2 (ja) 2004-01-06
CA2149903C (en) 2004-03-02
CA2149903A1 (en) 1995-04-13
AU7835394A (en) 1995-05-01
EP0672249B1 (de) 1999-05-12
WO1995010041A1 (en) 1995-04-13
DE69418450T2 (de) 1999-09-16
ES2131708T3 (es) 1999-08-01
US5490909A (en) 1996-02-13
ATE180056T1 (de) 1999-05-15
EP0672249A1 (de) 1995-09-20

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee