ATE440285T1 - Protein-färbung mittels merocyanin farbstoffe - Google Patents
Protein-färbung mittels merocyanin farbstoffeInfo
- Publication number
- ATE440285T1 ATE440285T1 AT96920325T AT96920325T ATE440285T1 AT E440285 T1 ATE440285 T1 AT E440285T1 AT 96920325 T AT96920325 T AT 96920325T AT 96920325 T AT96920325 T AT 96920325T AT E440285 T1 ATE440285 T1 AT E440285T1
- Authority
- AT
- Austria
- Prior art keywords
- merocyanine dyes
- detected
- amino acids
- poly
- peptides
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
- G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6827—Total protein determination, e.g. albumin in urine
- G01N33/6839—Total protein determination, e.g. albumin in urine involving dyes, e.g. Coomassie blue, bromcresol green
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/25125—Digestion or removing interfering materials
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Immunology (AREA)
- Pathology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Hematology (AREA)
- General Physics & Mathematics (AREA)
- Biophysics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Computational Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Sampling And Sample Adjustment (AREA)
- Pyridine Compounds (AREA)
- Plural Heterocyclic Compounds (AREA)
- Indole Compounds (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Detergent Compositions (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/444,895 US5616502A (en) | 1995-05-19 | 1995-05-19 | Non-specific protein staining using merocyanine dyes |
| PCT/US1996/007297 WO1996036882A1 (en) | 1995-05-19 | 1996-05-20 | Merocyanine dye protein stains |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE440285T1 true ATE440285T1 (de) | 2009-09-15 |
Family
ID=23766788
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT96920325T ATE440285T1 (de) | 1995-05-19 | 1996-05-20 | Protein-färbung mittels merocyanin farbstoffe |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US5616502A (de) |
| EP (1) | EP0774122B1 (de) |
| JP (4) | JP4079381B2 (de) |
| AT (1) | ATE440285T1 (de) |
| AU (1) | AU699961B2 (de) |
| CA (1) | CA2193705C (de) |
| DE (1) | DE69638004D1 (de) |
| WO (1) | WO1996036882A1 (de) |
Families Citing this family (69)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5912257A (en) * | 1995-09-06 | 1999-06-15 | The Research Foundation Of State University Of New York | Two-photon upconverting dyes and applications |
| US20030070927A1 (en) * | 1996-07-24 | 2003-04-17 | Merchant Mark E. | Reagents and methods for fluorescent analysis of serum proteins |
| ES2308800T3 (es) * | 1997-01-08 | 2008-12-01 | Invitrogen Corporation | Metodos para la produccion de proteinas. |
| WO1999007876A2 (de) * | 1997-08-12 | 1999-02-18 | Byk Gulden Lomberg Chemische Fabrik Gmbh | Verfahren zur bestimmung von zellulärem protein |
| WO1999040536A1 (en) * | 1998-02-10 | 1999-08-12 | Ey Laboratories, Inc. | Reflectometry system with compensation for specimen holder topography and with lock-rejection of system noise |
| US6485981B1 (en) * | 1998-07-29 | 2002-11-26 | Ciencia, Inc. | Method and apparatus for imaging and documenting fingerprints |
| US6372512B1 (en) | 1998-09-16 | 2002-04-16 | Resolution Sciences Corporation | Combined en bloc staining and embedding process |
| US6335446B1 (en) * | 1998-10-05 | 2002-01-01 | Oxford Glycosciences (Uk) Ltd. | Quinolinium- and pyridinium-based fluorescent dye compounds |
| WO2000025139A1 (en) | 1998-10-27 | 2000-05-04 | Molecular Probes, Inc. | Luminescent protein stains containing transition metal complexes |
| US20050027111A1 (en) * | 1999-02-02 | 2005-02-03 | Caliper Life Sciences, Inc. | Methods, devices and systems for characterizing proteins |
| AU758309B2 (en) * | 1999-02-02 | 2003-03-20 | Caliper Life Sciences, Inc. | Methods, devices and systems for characterizing proteins |
| US6438398B1 (en) * | 1999-03-05 | 2002-08-20 | The University Of Miami | Process and device for measuring tear fluorescein clearance |
| US6664047B1 (en) * | 1999-04-30 | 2003-12-16 | Molecular Probes, Inc. | Aza-benzazolium containing cyanine dyes |
| US6329205B1 (en) | 1999-08-31 | 2001-12-11 | Molecular Probes, Inc. | Detection method using luminescent europium-based protein stains |
| US6287758B1 (en) * | 2000-03-23 | 2001-09-11 | Axiom Biotechnologies, Inc. | Methods of registering trans-membrane electric potentials |
| US6579718B1 (en) | 2000-08-04 | 2003-06-17 | Molecular Probes, Inc. | Carbazolylvinyl dye protein stains |
| AU2001296502B2 (en) | 2000-10-02 | 2005-06-09 | Molecular Probes, Inc. | Reagents for labeling biomolecules having aldehyde or ketone moieties |
| US7723123B1 (en) | 2001-06-05 | 2010-05-25 | Caliper Life Sciences, Inc. | Western blot by incorporating an affinity purification zone |
| ATE259801T1 (de) * | 2001-07-03 | 2004-03-15 | Dyomics Gmbh | Verbindungen auf der basis von 2-bzw. 4- chromenyliden-merocyaninen und ihre verwendung |
| AU2003222668A1 (en) * | 2002-04-25 | 2003-11-10 | Rapidheal, Inc. | Growth factor delivery system for the healing of wounds and the prevention of inflammation and disease |
| US20030203032A1 (en) * | 2002-04-25 | 2003-10-30 | Schultz Clyde L. | Growth factor delivery system for the healing of wounds and the prevention of inflammation and disease |
| US20040171034A1 (en) * | 2002-05-03 | 2004-09-02 | Brian Agnew | Compositions and methods for detection and isolation of phosphorylated molecules |
| CA2483868A1 (en) * | 2002-05-03 | 2004-05-21 | Molecular Probes, Inc. | Compositions and methods for detection and isolation of phosphorylated molecules |
| US7445894B2 (en) * | 2002-05-03 | 2008-11-04 | Molecular Probes, Inc. | Compositions and methods for detection and isolation of phosphorylated molecules |
| JP2005532434A (ja) * | 2002-07-01 | 2005-10-27 | グアヴァ テクノロジーズ,インコーポレーテッド | 蛍光色素、エネルギー転移ペア及び方法 |
| EP1544618B1 (de) * | 2002-07-25 | 2009-03-18 | Ajinomoto Co., Inc. | Proteinanalyseverfahren |
| AU2003270619A1 (en) * | 2002-09-12 | 2004-04-30 | Molecular Probes, Inc. | Site-specific labeling of affinity tags in fusion proteins |
| WO2004025268A2 (en) * | 2002-09-13 | 2004-03-25 | Carnegie Mellon University | Optical biosensors and methods of use thereof |
| US20050287661A1 (en) * | 2002-10-08 | 2005-12-29 | James Landers | Methods and systems for multiplexing ir-mediated heating on a microchip |
| WO2004038423A2 (en) * | 2002-10-23 | 2004-05-06 | National Diagnostics, Inc. | In-gel fluorescent protein staining technique |
| US9216106B2 (en) | 2003-04-09 | 2015-12-22 | Directcontact Llc | Device and method for the delivery of drugs for the treatment of posterior segment disease |
| US20050255144A1 (en) * | 2003-04-09 | 2005-11-17 | Directcontact Llc | Methods and articles for the delivery of medicaments to the eye for the treatment of posterior segment diseases |
| US20050074497A1 (en) * | 2003-04-09 | 2005-04-07 | Schultz Clyde L. | Hydrogels used to deliver medicaments to the eye for the treatment of posterior segment diseases |
| EP1720944B1 (de) * | 2003-12-05 | 2013-07-17 | Life Technologies Corporation | Cyanin farbstoffverbindungen |
| US7776529B2 (en) | 2003-12-05 | 2010-08-17 | Life Technologies Corporation | Methine-substituted cyanine dye compounds |
| US7625758B2 (en) * | 2005-01-26 | 2009-12-01 | Berkelman Thomas R | Coumarin-based cyanine dyes for non-specific protein binding |
| DE102005004745A1 (de) | 2005-01-31 | 2006-08-10 | Dyomics Gmbh | Verfahren zu Nachweis und Quantifizierung von Proteinen |
| WO2006083846A2 (en) * | 2005-02-01 | 2006-08-10 | Sigma-Aldrich Co. | Detection of polyamino acids using trimethincyanine dyes |
| US7598390B2 (en) * | 2005-05-11 | 2009-10-06 | Life Technologies Corporation | Fluorescent chemical compounds having high selectivity for double stranded DNA, and methods for their use |
| JP4822409B2 (ja) * | 2005-10-19 | 2011-11-24 | 独立行政法人産業技術総合研究所 | タンパク質の分析方法 |
| JP2009524832A (ja) | 2006-01-24 | 2009-07-02 | ライフ テクノロジーズ コーポレーション | 検体を定量するためのデバイスおよび方法 |
| US9297807B2 (en) | 2006-02-10 | 2016-03-29 | Agilent Technologies, Inc. | Protein analysis using a polymethine marker dye |
| US8562802B1 (en) | 2006-02-13 | 2013-10-22 | Life Technologies Corporation | Transilluminator base and scanner for imaging fluorescent gels, charging devices and portable electrophoresis systems |
| GB0611116D0 (en) | 2006-06-06 | 2006-07-19 | Oxford Genome Sciences Uk Ltd | Proteins |
| EP2069386A4 (de) | 2006-07-21 | 2009-10-28 | Life Technologies Corp | Proteinmolekülgewichtsstandards mit hoher trennschärfe |
| DE112006003967B4 (de) | 2006-07-28 | 2017-09-21 | Pierce Biotechnology, Inc. | Farbstoffe, Farbstoffzusammensetzungen und Verfahren zum Anfärben von Proteinen |
| US8155730B2 (en) | 2006-10-24 | 2012-04-10 | The Research Foundation Of State University Of New York | Composition, method, system, and kit for optical electrophysiology |
| EP2629094A1 (de) | 2007-01-24 | 2013-08-21 | Carnegie Mellon University | Optische Biosensoren |
| WO2008104803A2 (en) | 2007-02-26 | 2008-09-04 | Oxford Genome Sciences (Uk) Limited | Proteins |
| EP2121745A2 (de) | 2007-02-26 | 2009-11-25 | Oxford Genome Sciences (UK) Limited | Proteine |
| WO2009067603A1 (en) | 2007-11-21 | 2009-05-28 | Bio-Rad Laboratories, Inc. | Photoluminescent metal complexes for protein staining |
| WO2009079212A2 (en) | 2007-12-03 | 2009-06-25 | Carnegie Mellon University | Linked peptide fluorogenic biosensors |
| EP2496605A1 (de) | 2009-11-02 | 2012-09-12 | Oxford Biotherapeutics Ltd. | Ror1 als therapeutisches und diagnostisches ziel |
| KR101111351B1 (ko) * | 2009-12-01 | 2012-06-12 | 주식회사 인트론바이오테크놀로지 | 합성 비이온성의 친수성 고분자에 기반한 미리 염색된 단백질 크기 마커 |
| US8974651B2 (en) | 2010-04-17 | 2015-03-10 | C.C. Imex | Illuminator for visualization of fluorophores |
| JP2012031082A (ja) * | 2010-07-29 | 2012-02-16 | National Institute Of Advanced Industrial Science & Technology | タンパク質を検出するための分析試薬 |
| AU2012309824A1 (en) | 2011-09-12 | 2013-04-18 | Abbvie Biotherapeutics Inc. | Artificial NK cells and uses thereof |
| US10024863B2 (en) * | 2013-03-15 | 2018-07-17 | Life Technologies Corporation | Methods for dye selection for protein melt temperature determinations |
| US9835587B2 (en) | 2014-04-01 | 2017-12-05 | C.C. Imex | Electrophoresis running tank assembly |
| CN105062467B (zh) * | 2015-08-07 | 2017-04-26 | 山东大学 | 一种转子型的双红线粒体荧光探针及其应用 |
| WO2019051252A1 (en) * | 2017-09-07 | 2019-03-14 | Bristol-Myers Squibb Company | METHOD FOR DETERMINING PURITY BY CAPILLARY ELECTROPHORESIS |
| WO2019213543A1 (en) | 2018-05-04 | 2019-11-07 | Li-Cor, Inc. | 2-[2-[4-[bis(2-sulfoethyl)amino]phenyl]ethenyl]-1-butyl-3,3-dimethyl-3h-indolium hemicyanine dyes for the detection of antibodies and other biomolecules |
| US11186719B2 (en) | 2018-05-04 | 2021-11-30 | Li-Cor, Inc. | Hemicyanine dyes |
| WO2020028639A1 (en) | 2018-08-01 | 2020-02-06 | Essen Instruments, Inc. D/B/A Essen Bioscience, Inc. | Methods, kits and stain compositions for flow cytometry evaluation of unassociated virus-size particles using multiple fluorogenic dyes |
| WO2020197644A1 (en) | 2019-03-26 | 2020-10-01 | Essen Instruments, Inc. D/B/A Essen Bioscience, Inc. | Flow cytometry evaluation for unassociated non-enveloped viral particles |
| US11709116B2 (en) | 2020-02-04 | 2023-07-25 | Sartorius Bioanalytical Instruments, Inc. | Liquid flourescent dye concentrate for flow cytometry evaluation of virus-size particles and related products and methods |
| EP4427033A1 (de) | 2021-11-07 | 2024-09-11 | PAIA Biotech GmbH | Verfahren zur qualitativen und quantitativen bestimmung von proteinaggregaten |
| FR3130978B1 (fr) * | 2021-12-16 | 2024-08-23 | Commissariat Energie Atomique | Utilisation d’un compose specifique pour la detection de composes chimiques toxiques |
| FR3154185A1 (fr) * | 2023-10-16 | 2025-04-18 | Commissariat A L'energie Atomique Et Aux Energies Alternatives | Système colorimétrique et fluorogénique pour la détection d’une substance chimique et procédé de détection d’une substance chimique le mettant en œuvre |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4424201A (en) * | 1978-11-28 | 1984-01-03 | Rockefeller University | Employment of a mereyanine dye for the detection of malignant leukocytic cells |
| JPS55116259A (en) * | 1979-03-01 | 1980-09-06 | Fuji Photo Film Co Ltd | Microimmunoassay method |
| US4886744A (en) * | 1985-04-25 | 1989-12-12 | Polaroid Corporation | Fluorescent conjugates and biological diagnostic assay system |
| US5268486A (en) * | 1986-04-18 | 1993-12-07 | Carnegie-Mellon Unversity | Method for labeling and detecting materials employing arylsulfonate cyanine dyes |
| DE3912046B4 (de) * | 1988-09-02 | 2004-03-25 | Carnegie Mellon University | Verfahren zum Markieren einer Komponente einer wäßrigen Flüssigkeit und lumineszenzphotostabiles Reaktionsprodukt |
| US5122602A (en) * | 1989-02-13 | 1992-06-16 | Miles Inc. | Chromogenic merocyanine enzyme substrates |
| ATA194989A (de) * | 1989-08-17 | 1990-04-15 | Koller Ernst | Verfahren zum nachweis und zur bestimmung von humanserumalbumin in biologischen fluessigkeiten mit hilfe fluorogener cyanin-farbstoffe |
| JPH05281191A (ja) * | 1991-04-25 | 1993-10-29 | Toyobo Co Ltd | 多標識系2次元電気泳動法 |
| US5264589A (en) * | 1991-06-06 | 1993-11-23 | Miles Inc. | Merocyanine protein error indicators |
| DE69213826T2 (de) * | 1991-06-06 | 1997-01-30 | Bayer Ag | Teststreifen mit Merocyanin und Nitro- oder Nitroso-substituierten polyhalogenierten Phenolsulfonphthaleinen als Protein-Indikatoren |
| DE69219610T2 (de) * | 1991-09-16 | 1997-10-02 | Molecular Probes Inc | Dimere unsymmetrische Cyaninfarbstoffe |
| US5321130A (en) * | 1992-02-10 | 1994-06-14 | Molecular Probes, Inc. | Unsymmetrical cyanine dyes with a cationic side chain |
| JPH05322770A (ja) * | 1992-05-15 | 1993-12-07 | Toyobo Co Ltd | 多標識系電気泳動法 |
| JPH05322771A (ja) * | 1992-05-15 | 1993-12-07 | Toyobo Co Ltd | 多標識系電気泳動システム |
| JP2698730B2 (ja) * | 1992-08-20 | 1998-01-19 | 松下電器産業株式会社 | 標識色素とその前駆体、その合成方法および標識色素を用いたメタンフェタミンの検出方法 |
| JP2653615B2 (ja) * | 1992-11-16 | 1997-09-17 | 松下電器産業株式会社 | 標識色素とその前駆体化合物及び標識色素の製造方法並びに標識色素の使用方法 |
| JP3354975B2 (ja) * | 1992-10-06 | 2002-12-09 | イビデン株式会社 | 蛍光標識試薬および蛍光免疫測定法 |
| JPH06123740A (ja) * | 1992-10-12 | 1994-05-06 | Canon Inc | 蛋白質標識用(メロ)シアニン色素、及び色素標識蛋白質 |
| JP3260884B2 (ja) * | 1993-02-05 | 2002-02-25 | キヤノン株式会社 | 標識複合体、それを用いる分析法、生体関連物質の検出および定量の方法 |
| US5410030A (en) * | 1993-04-05 | 1995-04-25 | Molecular Probes, Inc. | Dimers of unsymmetrical cyanine dyes containing pyridinium moieties |
| JPH06313118A (ja) * | 1993-04-30 | 1994-11-08 | Matsushita Electric Ind Co Ltd | 標識色素及び標識色素の中間物 |
-
1995
- 1995-05-19 US US08/444,895 patent/US5616502A/en not_active Expired - Lifetime
-
1996
- 1996-05-20 DE DE69638004T patent/DE69638004D1/de not_active Expired - Lifetime
- 1996-05-20 EP EP96920325A patent/EP0774122B1/de not_active Expired - Lifetime
- 1996-05-20 JP JP53513296A patent/JP4079381B2/ja not_active Expired - Fee Related
- 1996-05-20 WO PCT/US1996/007297 patent/WO1996036882A1/en not_active Ceased
- 1996-05-20 CA CA002193705A patent/CA2193705C/en not_active Expired - Lifetime
- 1996-05-20 AU AU58669/96A patent/AU699961B2/en not_active Expired
- 1996-05-20 AT AT96920325T patent/ATE440285T1/de not_active IP Right Cessation
-
2004
- 2004-04-21 JP JP2004126171A patent/JP2004271536A/ja not_active Withdrawn
- 2004-10-27 JP JP2004313169A patent/JP2005099031A/ja not_active Withdrawn
-
2007
- 2007-10-10 JP JP2007264888A patent/JP2008096437A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| DE69638004D1 (de) | 2009-10-01 |
| CA2193705C (en) | 2003-12-02 |
| JP4079381B2 (ja) | 2008-04-23 |
| JPH10504108A (ja) | 1998-04-14 |
| CA2193705A1 (en) | 1996-11-21 |
| AU5866996A (en) | 1996-11-29 |
| WO1996036882A1 (en) | 1996-11-21 |
| JP2008096437A (ja) | 2008-04-24 |
| US5616502A (en) | 1997-04-01 |
| AU699961B2 (en) | 1998-12-17 |
| JP2004271536A (ja) | 2004-09-30 |
| EP0774122A1 (de) | 1997-05-21 |
| EP0774122B1 (de) | 2009-08-19 |
| JP2005099031A (ja) | 2005-04-14 |
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