DE69033067D1 - 4,7-dichlorfluoreszein-farbstoffe als molekulare sonden - Google Patents

4,7-dichlorfluoreszein-farbstoffe als molekulare sonden

Info

Publication number
DE69033067D1
DE69033067D1 DE69033067T DE69033067T DE69033067D1 DE 69033067 D1 DE69033067 D1 DE 69033067D1 DE 69033067 T DE69033067 T DE 69033067T DE 69033067 T DE69033067 T DE 69033067T DE 69033067 D1 DE69033067 D1 DE 69033067D1
Authority
DE
Germany
Prior art keywords
dyes
dichlorfluoreszein
molecular probe
dichlorofluoresceins
fluorecein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69033067T
Other languages
English (en)
Other versions
DE69033067T2 (de
Inventor
Steven Menchen
Linda Lee
Charles Connell
N Hershey
Vergine Chakerian
Sam Woo
Steven Fung
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems LLC
Original Assignee
Perkin Elmer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Perkin Elmer Corp filed Critical Perkin Elmer Corp
Application granted granted Critical
Publication of DE69033067D1 publication Critical patent/DE69033067D1/de
Publication of DE69033067T2 publication Critical patent/DE69033067T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
    • C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
    • C07D311/78—Ring systems having three or more relevant rings
    • C07D311/80—Dibenzopyrans; Hydrogenated dibenzopyrans
    • C07D311/82—Xanthenes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B11/00—Diaryl- or thriarylmethane dyes
    • C09B11/04—Diaryl- or thriarylmethane dyes derived from triarylmethanes, i.e. central C-atom is substituted by amino, cyano, alkyl
    • C09B11/06—Hydroxy derivatives of triarylmethanes in which at least one OH group is bound to an aryl nucleus and their ethers or esters
    • C09B11/08—Phthaleins; Phenolphthaleins; Fluorescein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/968—High energy substrates, e.g. fluorescent, chemiluminescent, radioactive
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/80—Fluorescent dyes, e.g. rhodamine

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Photoreceptors In Electrophotography (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DE69033067T 1989-11-14 1990-11-13 4,7-dichlorfluoreszein-farbstoffe als molekulare sonden Expired - Lifetime DE69033067T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/436,455 US5188934A (en) 1989-11-14 1989-11-14 4,7-dichlorofluorescein dyes as molecular probes
PCT/US1990/006608 WO1991007507A1 (en) 1989-11-14 1990-11-13 4,7-dichlorofluorescein dyes as molecular probes

Publications (2)

Publication Number Publication Date
DE69033067D1 true DE69033067D1 (de) 1999-05-27
DE69033067T2 DE69033067T2 (de) 1999-10-21

Family

ID=23732469

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69033067T Expired - Lifetime DE69033067T2 (de) 1989-11-14 1990-11-13 4,7-dichlorfluoreszein-farbstoffe als molekulare sonden

Country Status (6)

Country Link
US (1) US5188934A (de)
EP (1) EP0500779B1 (de)
JP (1) JP2523423B2 (de)
AT (1) ATE179215T1 (de)
DE (1) DE69033067T2 (de)
WO (1) WO1991007507A1 (de)

Families Citing this family (266)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5821058A (en) * 1984-01-16 1998-10-13 California Institute Of Technology Automated DNA sequencing technique
USRE43096E1 (en) * 1984-01-16 2012-01-10 California Institute Of Technology Tagged extendable primers and extension products
US5360523A (en) * 1984-03-29 1994-11-01 Li-Cor, Inc. DNA sequencing
US5571388A (en) * 1984-03-29 1996-11-05 Li-Cor, Inc. Sequencing near infrared and infrared fluorescense labeled DNA for detecting using laser diodes and suitable labels thereof
US6004446A (en) * 1984-03-29 1999-12-21 Li-Cor, Inc. DNA Sequencing
US6207421B1 (en) 1984-03-29 2001-03-27 Li-Cor, Inc. DNA sequencing and DNA terminators
US5863403A (en) * 1984-03-29 1999-01-26 The Board Of Regents Of The University Of Nebraska Digital DNA typing
US6086737A (en) * 1984-03-29 2000-07-11 Li-Cor, Inc. Sequencing near infrared and infrared fluorescence labeled DNA for detecting using laser diodes and suitable labels therefor
US4729947A (en) * 1984-03-29 1988-03-08 The Board Of Regents Of The University Of Nebraska DNA sequencing
US5654442A (en) 1989-11-14 1997-08-05 The Perkin-Elmer Corporation 4,7-dichlorofluorescein dyes as molecular probes
DE69324494T2 (de) * 1992-09-03 1999-09-23 The Perkin-Elmer Corp., Foster City 4,7-dichlorofluoroszein-farbestoffe als molekulare sonden
US5869255A (en) * 1994-02-01 1999-02-09 The Regents Of The University Of California Probes labeled with energy transfer couples dyes exemplified with DNA fragment analysis
US5654419A (en) * 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
US6028190A (en) * 1994-02-01 2000-02-22 The Regents Of The University Of California Probes labeled with energy transfer coupled dyes
US5604097A (en) 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
US5582705A (en) * 1995-05-19 1996-12-10 Iowa State University Research Foundation, Inc. Multiplexed capillary electrophoresis system
US5861287A (en) * 1995-06-23 1999-01-19 Baylor College Of Medicine Alternative dye-labeled primers for automated DNA sequencing
US5728529A (en) * 1995-06-23 1998-03-17 Baylor College Of Medicine Alternative dye-labeled ribonucleotides, deoxyribonucleotides, and dideoxyribonucleotides for automated DNA analysis
US5614386A (en) * 1995-06-23 1997-03-25 Baylor College Of Medicine Alternative dye-labeled primers for automated DNA sequencing
US5641633A (en) * 1995-11-15 1997-06-24 Becton, Dickinson And Company Fluorescence polarization detection of nucleic acids
US6020481A (en) 1996-04-01 2000-02-01 The Perkin-Elmer Corporation Asymmetric benzoxanthene dyes
US6162931A (en) * 1996-04-12 2000-12-19 Molecular Probes, Inc. Fluorinated xanthene derivatives
US7825237B2 (en) * 1996-05-03 2010-11-02 Applied Biosystems, Llc Oligonucleotides and analogs labeled with energy transfer dyes
US5863727A (en) * 1996-05-03 1999-01-26 The Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US5847162A (en) * 1996-06-27 1998-12-08 The Perkin Elmer Corporation 4, 7-Dichlororhodamine dyes
US5800996A (en) * 1996-05-03 1998-09-01 The Perkin Elmer Corporation Energy transfer dyes with enchanced fluorescence
US7388092B2 (en) * 1996-05-03 2008-06-17 Applera Corporation Oligonucleotides and analogs labeled with energy transfer dyes
US5945526A (en) 1996-05-03 1999-08-31 Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US20040266706A1 (en) * 2002-11-05 2004-12-30 Muthiah Manoharan Cross-linked oligomeric compounds and their use in gene modulation
US7550570B2 (en) * 2000-05-25 2009-06-23 Applied Biosystems, Llc. 4,7-dichlororhodamine dyes labeled polynucleotides
US6017712A (en) * 1996-06-27 2000-01-25 Lee; Linda 4,7-dichlororhodamine dyes
US6080852A (en) * 1996-06-27 2000-06-27 The Perkin-Elmer Corporation 4,7-dichlororhodamine dyes
US5821356A (en) * 1996-08-12 1998-10-13 The Perkin Elmer Corporation Propargylethoxyamino nucleotides
US5853992A (en) * 1996-10-04 1998-12-29 The Regents Of The University Of California Cyanine dyes with high-absorbance cross section as donor chromophores in energy transfer labels
AU762888B2 (en) * 1997-02-12 2003-07-10 Us Genomics Methods and products for analyzing polymers
US6130101A (en) * 1997-09-23 2000-10-10 Molecular Probes, Inc. Sulfonated xanthene derivatives
US6008379A (en) * 1997-10-01 1999-12-28 The Perkin-Elmer Corporation Aromatic-substituted xanthene dyes
US5958694A (en) * 1997-10-16 1999-09-28 Caliper Technologies Corp. Apparatus and methods for sequencing nucleic acids in microfluidic systems
US6289229B1 (en) 1998-01-20 2001-09-11 Scimed Life Systems, Inc. Readable probe array for in vivo use
US6080868A (en) 1998-01-23 2000-06-27 The Perkin-Elmer Corporation Nitro-substituted non-fluorescent asymmetric cyanine dye compounds
US6653462B2 (en) 1998-05-29 2003-11-25 Applera Corporation Nucleotide compounds including a rigid linker
US5948648A (en) * 1998-05-29 1999-09-07 Khan; Shaheer H. Nucleotide compounds including a rigid linker
US6096875A (en) 1998-05-29 2000-08-01 The Perlein-Elmer Corporation Nucleotide compounds including a rigid linker
US6908770B1 (en) 1998-07-16 2005-06-21 Board Of Regents, The University Of Texas System Fluid based analysis of multiple analytes by a sensor array
US6153411A (en) * 1998-10-30 2000-11-28 American Water Works Company, Inc. Methods and kits for detection of Cryptosporidium parvum using immunomagnetic separation and amplification
US6432642B1 (en) * 1999-01-15 2002-08-13 Pe Corporation (Ny) Binary probe and clamp composition and methods for a target hybridization detection
US7022517B1 (en) 1999-07-16 2006-04-04 Board Of Regents, The University Of Texas System Method and apparatus for the delivery of samples to a chemical sensor array
DE60031988T2 (de) 1999-07-16 2007-06-14 The Board Of Regents Of The University Of Texas System, Austin Verfahren und vorrichtung zur zuführung von proben zu einer chemischen sensormatrix
US6358684B1 (en) * 1999-08-27 2002-03-19 Pe Corporation UV excitable fluorescent energy transfer dyes
US6218124B1 (en) 1999-08-27 2001-04-17 Pe Corporation Method for detecting oligonucleotides using UV light source
US6569685B1 (en) 1999-10-05 2003-05-27 The Molecular Sciences Institute, Inc. Protein fingerprint system and related methods
AU2001247195A1 (en) * 2000-01-31 2001-08-07 Board Of Regents, The University Of Texas System Method and system for collecting and transmitting chemical information
US6221604B1 (en) 2000-02-07 2001-04-24 Pe Corporation Electron-deficient nitrogen heterocycle-substituted fluorescein dyes
US6887664B2 (en) 2000-06-06 2005-05-03 Applera Corporation Asynchronous primed PCR
ATE431355T1 (de) * 2000-10-11 2009-05-15 Applera Corp Fluoreszierende nukleobasekonjugate mit anionische linker
US7211414B2 (en) 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
EP1373874A4 (de) * 2001-01-31 2004-03-31 Univ Texas Verfahren und vorrichtung zum eingrenzen von materialien in einem mikrobearbeiteten chemischen sensorarray
US20030003436A1 (en) * 2001-02-05 2003-01-02 Willson C. Grant Use of mesoscale self-assembly and recognition to effect delivery of sensing reagent for arrayed sensors
EP1748078B1 (de) * 2001-03-09 2011-01-19 Boston Probes, Inc. Kombinationsoligomere betreffende Verfahren, Kits und Zusammensetzungen
US6887690B2 (en) 2001-06-22 2005-05-03 Pe Corporation Dye-labeled ribonucleotide triphosphates
US7668697B2 (en) * 2006-02-06 2010-02-23 Andrei Volkov Method for analyzing dynamic detectable events at the single molecule level
US7052512B2 (en) * 2001-07-18 2006-05-30 Boston Scientific Scimed, Inc. Fluorescent dyed lubricant for medical devices
US6764710B2 (en) 2001-07-18 2004-07-20 Scimed Life Systems, Inc. Light emitting markers for use with substrates
JP2004537053A (ja) * 2001-07-25 2004-12-09 アプレラ コーポレイション 電気泳動検出システムにおける時間遅延積分
US20050032060A1 (en) * 2001-08-31 2005-02-10 Shishir Shah Arrays comprising pre-labeled biological molecules and methods for making and using these arrays
US8569516B2 (en) * 2001-09-07 2013-10-29 Elitech Holding B.V. Compounds and methods for fluorescent labeling
US6972339B2 (en) 2001-09-07 2005-12-06 Epoch Biosciences, Inc. Compounds and methods for fluorescent labeling
US7439346B2 (en) * 2001-10-12 2008-10-21 Perkinelmer Las Inc. Nucleic acids arrays and methods of use therefor
JP2005519634A (ja) 2001-10-12 2005-07-07 スペクトラル ジェノミクス、インク． 核酸組成物及びアレイ及びそれらを用いる方法
EP1448800A4 (de) 2001-11-21 2007-05-16 Applera Corp Digitaler assay
WO2003066812A2 (en) * 2002-02-05 2003-08-14 Baylor College Of Medecine Substituted 4,4-difluoro-4-bora-3a, 4a-diaza-s-indacene compounds for 8-color dna sequencing
US6916621B2 (en) * 2002-03-27 2005-07-12 Spectral Genomics, Inc. Methods for array-based comparitive binding assays
AU2003228395B2 (en) 2002-04-02 2006-12-21 Caliper Life Sciences, Inc. Methods and apparatus for separation and isolation of components from a biological sample
US7150967B2 (en) * 2002-04-08 2006-12-19 University Of Medicine & Dentistry Of New Jersey Fluorescent tags for amino acid and nucleic acid analysis
CA2523626A1 (en) 2002-04-26 2003-11-06 Board Of Regents, The University Of Texas System Method and system for the detection of cardiac risk factors
US20040137469A1 (en) 2002-09-08 2004-07-15 Casale Ralph A Methods, compositions and libraries pertaining PNA dimer and PNA oligomer synthesis
WO2004026804A1 (en) * 2002-09-20 2004-04-01 Integrated Dna Technologies, Inc. Anthraquinone quencher dyes, their methods of preparation and use
WO2004097371A2 (en) * 2003-04-25 2004-11-11 Board Of Regents, The University Of Texas System System and method for the detection of analytes
EP1627025B1 (de) 2003-05-09 2016-10-12 Applied Biosystems, LLC Fluoreszierende kunststoffe mit gehalt an lipidlöslichen rhodaminfarbstoffen
WO2004101709A1 (en) 2003-05-09 2004-11-25 Applera Corporation Phenyl xanthene dyes
US9317922B2 (en) 2003-05-16 2016-04-19 Board Of Regents The University Of Texas System Image and part recognition technology
US7651850B2 (en) * 2003-05-16 2010-01-26 Board Of Regents, The University Of Texas System Image and part recognition technology
US20060029948A1 (en) * 2003-09-19 2006-02-09 Gary Lim Sealing cover and dye compatibility selection
US20050112634A1 (en) * 2003-09-19 2005-05-26 Woudenberg Timothy M. High density sequence detection methods and apparatus
US7417726B2 (en) * 2003-09-19 2008-08-26 Applied Biosystems Inc. Normalization of data using controls
US7570443B2 (en) 2003-09-19 2009-08-04 Applied Biosystems, Llc Optical camera alignment
US7541454B2 (en) * 2003-10-24 2009-06-02 Epoch Biosciences, Inc. Compounds and methods for fluorescent labeling
EP1687609B1 (de) * 2003-10-28 2014-12-10 Epoch Biosciences, Inc. Fluoreszenzsonden zum nachweis von dna durch hybridisierung mit verbesserter empfindlichkeit und geringem hintergrund
US7439341B2 (en) 2003-11-14 2008-10-21 Integrated Dna Technologies, Inc. Fluorescence quenching azo dyes, their methods of preparation and use
PL1538154T3 (pl) * 2003-11-20 2009-06-30 Exiqon As Kompozycja wygaszacza zawierająca ugrupowania atrachinonu
JP4127204B2 (ja) * 2003-12-17 2008-07-30 セイコーエプソン株式会社 液晶表示装置の製造方法
EP1704160B1 (de) 2003-12-22 2010-11-10 The Trustees of The University of Pennsylvania Verfahren und zusammensetzungen zur identifikation von rna-bindenden proteinen
WO2005077125A2 (en) * 2004-02-11 2005-08-25 Applera Corporation Methods and compositions for detecting nucleic acids
US8101431B2 (en) * 2004-02-27 2012-01-24 Board Of Regents, The University Of Texas System Integration of fluids and reagents into self-contained cartridges containing sensor elements and reagent delivery systems
US8105849B2 (en) * 2004-02-27 2012-01-31 Board Of Regents, The University Of Texas System Integration of fluids and reagents into self-contained cartridges containing sensor elements
US20050255485A1 (en) * 2004-05-14 2005-11-17 Livak Kenneth J Detection of gene duplications
JP5435864B2 (ja) 2004-05-28 2014-03-05 アシュラジェン インコーポレイテッド マイクロｒｎａに関与する方法および組成物
WO2006016978A1 (en) * 2004-06-30 2006-02-16 Applera Corporation Analog probe complexes
EP1781675B1 (de) 2004-08-13 2014-03-26 Epoch Biosciences, Inc. Fluoreszierende phosphonatfarbstoffe und konjugate
EP2284265B1 (de) 2004-11-12 2015-01-07 Asuragen, Inc. Verfahren und Zusammensetzungen, die miRNAs und miRNA-inhibitorischen Molekülen verbunden sind
US20060292586A1 (en) * 2004-12-17 2006-12-28 Schroth Gary P ID-tag complexes, arrays, and methods of use thereof
US20060166238A1 (en) * 2004-12-22 2006-07-27 Ramsing Niels B Probes, libraries and kits for analysis of mixtures of nucleic acids and methods for constructing the same
US20060228726A1 (en) * 2005-01-06 2006-10-12 Martin Patrick K Polypeptides having nucleic acid binding activity and compositions and methods for nucleic acid amplification
EP2230315A1 (de) 2005-02-01 2010-09-22 AB Advanced Genetic Analysis Corporation Nukleinsäuresequenzierung durch schrittweise Duplexverlängerung
EP2241637A1 (de) 2005-02-01 2010-10-20 AB Advanced Genetic Analysis Corporation Nukleinsäuresequenzierung durch schrittweise Duplexverlängerung
WO2006099537A2 (en) 2005-03-15 2006-09-21 Applera Corporation The use of antibody-surrogate antigen systems for detection of analytes
WO2006119368A2 (en) 2005-05-03 2006-11-09 Applera Corporation Fluorescent detection system and dye set for use therewith
US7476735B2 (en) 2005-05-20 2009-01-13 Integrated Dna Technologies, Inc. Compounds and methods for labeling oligonucleotides
US8377398B2 (en) 2005-05-31 2013-02-19 The Board Of Regents Of The University Of Texas System Methods and compositions related to determination and use of white blood cell counts
US20090264299A1 (en) * 2006-02-24 2009-10-22 Complete Genomics, Inc. High throughput genome sequencing on DNA arrays
US8445194B2 (en) 2005-06-15 2013-05-21 Callida Genomics, Inc. Single molecule arrays for genetic and chemical analysis
US20070087360A1 (en) * 2005-06-20 2007-04-19 Boyd Victoria L Methods and compositions for detecting nucleotides
EP1899450A4 (de) * 2005-06-24 2010-03-24 Univ Texas Systeme und verfahren einschliesslich in sich geschlossener kassetten mit nachweissystemen und flüssigkeitszufuhrsystemen
WO2007005666A2 (en) * 2005-07-01 2007-01-11 Board Of Regents, The University Of Texas System System and method of analyte detection using differential receptors
US20070059713A1 (en) 2005-09-09 2007-03-15 Lee Jun E SSB-DNA polymerase fusion proteins
US7741467B2 (en) * 2005-10-05 2010-06-22 Roche Molecular Systems, Inc. Non-fluorescent energy transfer
WO2007044245A2 (en) 2005-10-07 2007-04-19 Callida Genomics, Inc. Self-assembled single molecule arrays and uses thereof
EP1954838B1 (de) 2005-11-14 2014-02-26 Life Technologies Corporation Kodierte moleküle zur erkennung von zielanalyten
EP2495337A1 (de) * 2006-02-24 2012-09-05 Callida Genomics, Inc. Genomsequenzierung auf DNA-Arrays mit hohem Durchsatz
WO2007106907A2 (en) * 2006-03-15 2007-09-20 Integrated Dna Technologies, Inc. Nucleic acid monomers with 2'-chemical moieties
US9677123B2 (en) 2006-03-15 2017-06-13 Siemens Healthcare Diagnostics Inc. Degenerate nucleobase analogs
CA2649725A1 (en) * 2006-04-19 2007-10-25 Applera Corporation Reagents, methods, and libraries for gel-free bead-based sequencing
WO2007140015A2 (en) 2006-05-26 2007-12-06 Althea Technologies, Inc Biochemical analysis of partitioned cells
AU2007299748A1 (en) * 2006-09-19 2008-03-27 Asuragen, Inc. miR-15, miR-26, miR -31,miR -145, miR-147, miR-188, miR-215, miR-216 miR-331, mmu-miR-292-3p regulated genes and pathways as targets for therapeutic intervention
US7910354B2 (en) * 2006-10-27 2011-03-22 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US20090111706A1 (en) 2006-11-09 2009-04-30 Complete Genomics, Inc. Selection of dna adaptor orientation by amplification
US7897737B2 (en) * 2006-12-05 2011-03-01 Lasergen, Inc. 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
US7893227B2 (en) * 2006-12-05 2011-02-22 Lasergen, Inc. 3′-OH unblocked nucleotides and nucleosides base modified with non-cleavable, terminating groups and methods for their use in DNA sequencing
US20080131878A1 (en) * 2006-12-05 2008-06-05 Asuragen, Inc. Compositions and Methods for the Detection of Small RNA
CN101622350A (zh) * 2006-12-08 2010-01-06 奥斯瑞根公司 作为干预治疗靶标的miR-126调控基因和通路
CA2671294A1 (en) * 2006-12-08 2008-06-19 Asuragen, Inc. Mir-21 regulated genes and pathways as targets for therapeutic intervention
CN101675165A (zh) * 2006-12-08 2010-03-17 奥斯瑞根公司 Let-7微小rna的功能和靶标
AU2007333106A1 (en) * 2006-12-08 2008-06-19 Asuragen, Inc. miR-20 regulated genes and pathways as targets for therapeutic intervention
KR101571409B1 (ko) * 2006-12-11 2015-11-24 알콘 리서치, 리미티드 실질적으로 순수한 플루오레세인
US20090175827A1 (en) * 2006-12-29 2009-07-09 Byrom Mike W miR-16 REGULATED GENES AND PATHWAYS AS TARGETS FOR THERAPEUTIC INTERVENTION
CA2697357A1 (en) * 2007-04-16 2008-10-30 John T. Mcdevitt Cardibioindex/cardibioscore and utility of salivary proteome in cardiovascular diagnostics
US20080274458A1 (en) * 2007-05-01 2008-11-06 Latham Gary J Nucleic acid quantitation methods
US20090232893A1 (en) * 2007-05-22 2009-09-17 Bader Andreas G miR-143 REGULATED GENES AND PATHWAYS AS TARGETS FOR THERAPEUTIC INTERVENTION
EP2167138A2 (de) * 2007-06-08 2010-03-31 Asuragen, INC. Mir-34-regulierte gene und pfade als targets für therapeutische intervention
US7816135B2 (en) 2007-07-05 2010-10-19 Becton, Dickinson And Company Method of analyzing lymphocytes
EP2482076A1 (de) 2007-07-31 2012-08-01 Osaka University Zusammensetzung zum Messen der Bindeaffinität zwischen Nukleinsäure und Testsubstanz und deren Verwendung
WO2009036332A1 (en) * 2007-09-14 2009-03-19 Asuragen, Inc. Micrornas differentially expressed in cervical cancer and uses thereof
JP2010539982A (ja) * 2007-10-01 2010-12-24 アプライド バイオシステムズ， エルエルシー チェイスライゲーション配列決定法
US20090186015A1 (en) * 2007-10-18 2009-07-23 Latham Gary J Micrornas differentially expressed in lung diseases and uses thereof
US8017338B2 (en) 2007-11-20 2011-09-13 Life Technologies Corporation Reversible di-nucleotide terminator sequencing
WO2009067632A1 (en) * 2007-11-20 2009-05-28 Applied Biosystems Inc. Method of sequencing nucleic acids using elaborated nucleotide phosphorothiolate compounds
WO2009070805A2 (en) * 2007-12-01 2009-06-04 Asuragen, Inc. Mir-124 regulated genes and pathways as targets for therapeutic intervention
WO2009086156A2 (en) * 2007-12-21 2009-07-09 Asuragen, Inc. Mir-10 regulated genes and pathways as targets for therapeutic intervention
US20090263803A1 (en) * 2008-02-08 2009-10-22 Sylvie Beaudenon Mirnas differentially expressed in lymph nodes from cancer patients
AU2008353360A1 (en) * 2008-03-24 2009-10-01 The Board Of Regents Of The University Of Texas System Image-guided therapy of myocardial disease: composition, manufacturing and applications
US20090253780A1 (en) * 2008-03-26 2009-10-08 Fumitaka Takeshita COMPOSITIONS AND METHODS RELATED TO miR-16 AND THERAPY OF PROSTATE CANCER
US20090258928A1 (en) * 2008-04-08 2009-10-15 Asuragen, Inc. Methods and compositions for diagnosing and modulating human papillomavirus (hpv)
US8258111B2 (en) 2008-05-08 2012-09-04 The Johns Hopkins University Compositions and methods related to miRNA modulation of neovascularization or angiogenesis
EP2307565B1 (de) 2008-06-11 2017-11-29 Lasergen, Inc. Spaltbare markierte nukleoside und nukleotide und verfahren zu ihrer verwendung bei der dna-sequenzierung
WO2010056737A2 (en) * 2008-11-11 2010-05-20 Mirna Therapeutics, Inc. Methods and compositions involving mirnas in cancer stem cells
US20100159452A1 (en) * 2008-12-22 2010-06-24 Roche Molecular Systems, Inc. Method For Detecting a Target Nucleic Acid in a Sample
US20100294952A1 (en) * 2009-01-15 2010-11-25 Northwestern University Controlled agent release and sequestration
US20110014612A1 (en) 2009-03-27 2011-01-20 Life Technologies Corporation Polymerase compositions & methods
WO2010135384A2 (en) 2009-05-21 2010-11-25 Siemens Healthcare Diagnostics Inc. Universal tags with non-natural nucleobases
US8597892B2 (en) 2009-05-22 2013-12-03 Asuragen, Inc. MiRNA biomarkers of prostate disease
EP2470897A4 (de) 2009-08-28 2013-05-29 Asuragen Inc Mirna-biomarker für lungenerkrankungen
WO2011038004A1 (en) 2009-09-22 2011-03-31 The Trustees Of The University Of Pennsylvania Methods and compositions for in-vivo enzyme capture
GB0922377D0 (en) 2009-12-22 2010-02-03 Arab Gulf University The Mutant LDL receptor
US9506057B2 (en) 2010-03-26 2016-11-29 Integrated Dna Technologies, Inc. Modifications for antisense compounds
JP5886828B2 (ja) 2010-03-26 2016-03-16 インテグレイテッド ディーエヌエイ テクノロジーズ インコーポレイテッド 核酸のハイブリダイゼーションを強化する方法
US7956169B1 (en) 2010-06-16 2011-06-07 Chemgenes Corporation Synthesis of novel azo-dyes and their use in oligonucleotide synthesis
US9410194B2 (en) 2010-06-21 2016-08-09 Life Technologies Corporation Compositions, kits and methods for synthesis and/or detection of nucleic acids
US20130261003A1 (en) 2010-08-06 2013-10-03 Ariosa Diagnostics, In. Ligation-based detection of genetic variants
US20120034603A1 (en) 2010-08-06 2012-02-09 Tandem Diagnostics, Inc. Ligation-based detection of genetic variants
EP2614149B1 (de) 2010-09-07 2015-04-08 Integrated Dna Technologies, Inc. Modifizierungen für antisense-verbindungen
US9777330B2 (en) 2010-10-29 2017-10-03 Asuragen, Inc. mPCR methods for analyzing repeat sequences
EP2658998B1 (de) 2010-12-29 2015-04-22 Life Technologies Corporation Ddao-verbindungen als fluorezenzreferenzstandards
US20120190020A1 (en) 2011-01-25 2012-07-26 Aria Diagnostics, Inc. Detection of genetic abnormalities
US10131947B2 (en) 2011-01-25 2018-11-20 Ariosa Diagnostics, Inc. Noninvasive detection of fetal aneuploidy in egg donor pregnancies
WO2012118745A1 (en) 2011-02-28 2012-09-07 Arnold Oliphant Assay systems for detection of aneuploidy and sex determination
JP5893442B2 (ja) * 2011-03-04 2016-03-23 国立大学法人宇都宮大学 チロシナーゼ活性阻害剤
CN103635594B (zh) 2011-05-09 2018-02-13 富鲁达公司 基于探针的核酸检测
ES2570591T3 (es) 2011-05-24 2016-05-19 Elitechgroup B V Detección de Staphylococcus aureus resistente a meticilina
WO2013039883A1 (en) 2011-09-12 2013-03-21 Abbvie Biotherapeutics Inc. Artificial nk cells and uses thereof
US9644241B2 (en) 2011-09-13 2017-05-09 Interpace Diagnostics, Llc Methods and compositions involving miR-135B for distinguishing pancreatic cancer from benign pancreatic disease
US8889860B2 (en) 2011-09-13 2014-11-18 Lasergen, Inc. 3′-OH unblocked, fast photocleavable terminating nucleotides and methods for nucleic acid sequencing
US10501791B2 (en) 2011-10-14 2019-12-10 President And Fellows Of Harvard College Sequencing by structure assembly
US20150031577A1 (en) 2012-03-20 2015-01-29 UNIVERSITé LAVAL Nucleic acid detection method comprising target specific indexing probes (tsip) comprising a releasable segment to be detected via fluorescence when bound to a capture probe
CN104736722B (zh) 2012-05-21 2018-08-07 斯克利普斯研究所 样品制备方法
WO2013184754A2 (en) 2012-06-05 2013-12-12 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using dna origami probes
IN2014DN07866A (de) 2012-07-20 2015-04-24 Harvard College
US20160046997A1 (en) 2012-10-18 2016-02-18 Oslo Universitetssykehus Hf Biomarkers for cervical cancer
US9476089B2 (en) 2012-10-18 2016-10-25 President And Fellows Of Harvard College Methods of making oligonucleotide probes
WO2014093291A1 (en) 2012-12-10 2014-06-19 Advandx, Inc. Use of probes for mass spectrometric identification of microorganisms or cells and associated conditions of interest
US9289502B2 (en) 2013-03-08 2016-03-22 Emerald Therapeutics, Inc. Preparation of oligo conjugates
WO2014164479A1 (en) 2013-03-11 2014-10-09 Elitech Holding B.V. Methods for true isothermal strand displacement amplification
US9540685B2 (en) 2013-03-15 2017-01-10 President And Fellows Of Harvard College Methods of identifying homologous genes using FISH
WO2014152054A1 (en) 2013-03-15 2014-09-25 Bio-Rad Laboratories, Inc. Digital assays for mutation detection
EP2997161B1 (de) 2013-05-13 2017-09-27 Elitechgroup B.V. Digitale tröpfchen -pcr mit kurzen sonden mit kleineren rillen
EP3090059B1 (de) 2014-01-05 2018-06-06 Biomirna Holdings Ltd. Micro-rna quotienten zur bestimmung von lungenkrebs
WO2015120382A1 (en) 2014-02-07 2015-08-13 The Johns Hopkins University Predicting response to epigenetic drug therapy
CN107109474B (zh) 2014-08-19 2021-03-19 豪夫迈·罗氏有限公司 用于核酸检测的方法和组合物
WO2016028843A2 (en) 2014-08-19 2016-02-25 President And Fellows Of Harvard College Rna-guided systems for probing and mapping of nucleic acids
US10450562B2 (en) 2014-09-09 2019-10-22 Igenomx International Genomics Corporation Methods and compositions for rapid nucleic acid library preparation
KR20220100098A (ko) 2014-11-21 2022-07-14 나노스트링 테크놀로지스, 인크. 무효소 및 무증폭 시퀀싱
WO2016094330A2 (en) 2014-12-08 2016-06-16 20/20 Genesystems, Inc Methods and machine learning systems for predicting the liklihood or risk of having cancer
JP2017538419A (ja) 2014-12-12 2017-12-28 エリテックグループ・ベスローテン・フェンノートシャップＥｌｉｔｅｃｈｇｒｏｕｐ Ｂ．Ｖ． 抗生物質耐性菌を検出するための方法および組成物
JP2017538418A (ja) 2014-12-12 2017-12-28 エリテックグループ・ベスローテン・フェンノートシャップＥｌｉｔｅｃｈｇｒｏｕｐ Ｂ．Ｖ． 抗生物質耐性細菌を検出する方法および組成物
CN107636165A (zh) 2015-01-12 2018-01-26 生命科技公司 用于核酸文库质量控制和定量的方法、系统和其组合物
WO2016164407A2 (en) 2015-04-07 2016-10-13 Polyskope Labs Detection of one or more pathogens
EP3288563B1 (de) 2015-05-01 2019-11-13 Vanderbilt University Überwachung und analyse von nukleinsäurehybridisierungsstatus und amplifikation mit l-dna
EP3292214A1 (de) 2015-05-04 2018-03-14 Academisch Medisch Centrum Verfahren zur quantifizierung von mirnas mittels normalisierung
WO2017015543A1 (en) 2015-07-23 2017-01-26 Asuragen, Inc. Methods, compositions, kits, and uses for analysis of nucleic acids comprising repeating a/t-rich segments
WO2017044651A2 (en) 2015-09-10 2017-03-16 Beckman Coulter, Inc. Short oligonucleotide quenchers for reduction of taqman probe baseline fluorescence
JP2019515667A (ja) 2016-04-06 2019-06-13 ライフ テクノロジーズ コーポレーション 核酸の合成及び検出のための組成物、方法及びキット
JP7457457B2 (ja) 2016-05-16 2024-03-28 ナノストリング テクノロジーズ，インコーポレイティド サンプル中の標的核酸を検出する方法
PT3478832T (pt) 2016-07-01 2022-05-19 Carlsberg As Método para selecionar um mutante numa população de organismos por aplicação de uma abordagem de agrupamento e divisão
WO2018045162A1 (en) 2016-09-01 2018-03-08 Biogen Ma Inc. Biomarkers predictive of primary progressive multiple sclerosis and uses thereof
KR102695749B1 (ko) 2016-11-21 2024-08-14 나노스트링 테크놀로지스, 인크. 화학적 조성물 및 이것을 사용하는 방법
US20190032131A1 (en) 2016-12-12 2019-01-31 Integrated Dna Technologies, Inc. Genome editing detection
WO2018129214A1 (en) 2017-01-04 2018-07-12 Complete Genomics, Inc. Stepwise sequencing by non-labeled reversible terminators or natural nucleotides
WO2018223053A1 (en) 2017-06-02 2018-12-06 Affymetrix, Inc. Array-based methods for analysing mixed samples using differently labelled allele-specific probes
WO2018223055A1 (en) 2017-06-02 2018-12-06 Affymetrix, Inc. Array-based methods for analysing mixed samples using differently labelled allele-specific probes
WO2019104070A1 (en) 2017-11-21 2019-05-31 Nanostring Technologies, Inc. O-nitrobenzyl photocleavable bifunctional linker
US11820791B2 (en) 2018-03-14 2023-11-21 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
JP7525402B2 (ja) 2018-05-14 2024-07-30 ナノストリング テクノロジーズ，インコーポレイティド 化学的組成物とそれを利用する方法
JP2022515153A (ja) 2018-12-20 2022-02-17 ライフ テクノロジーズ コーポレーション 非対称ローダミン染料及びバイオアッセイにおけるそれらの使用
KR20210107737A (ko) 2018-12-20 2021-09-01 라이프 테크놀로지스 코포레이션 개질된 로다민 염료 및 생물학적 분석에서의 그 용도
EP3719144A1 (de) 2019-04-05 2020-10-07 Fundación para la Investigación Biomédica del Hospital Universitario de la Paz (FIBHULP) Mir-151a-3p als universelle endogene steuerung zur exosomenladungsnormalisierung
EP3999664A1 (de) 2019-07-19 2022-05-25 Fundación para la Investigación Biomédica del Hospital Universitario de la Paz (FIBHULP) Verfahren zur bestimmung des ansprechens auf die behandlung eines patienten mit nicht-kleinzelligem lungenkarzinom (nsclc)
IL292003A (en) 2019-10-10 2022-06-01 Carlsberg As A method for preparing mutant plants
JP2022552013A (ja) 2019-10-18 2022-12-14 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー 臨床規模および工業規模の無傷の組織の配列決定
WO2021080629A1 (en) 2019-10-23 2021-04-29 Elitechgroup, Inc. Methods for true isothermal strand displacement amplification
WO2021178893A2 (en) 2020-03-06 2021-09-10 Singular Genomics Systems, Inc. Linked paired strand sequencing
EP4153606A4 (de) 2020-07-13 2024-10-02 Singular Genomics Systems Inc Verfahren zur sequenzierung komplementärer polynukleotide
CN116323965A (zh) 2020-07-23 2023-06-23 生命技术公司 使用染料的用于生物分析的组合物、系统和方法
US20240060117A1 (en) 2020-07-23 2024-02-22 Life Technologies Corporation Energy transfer dye conjugates for use in biological assays
AU2021343471A1 (en) 2020-09-16 2023-05-04 Bruker Spatial Biology, Inc. Chemical compositions and methods of using the same
WO2022087485A1 (en) 2020-10-22 2022-04-28 Singular Genomics Systems, Inc. Nucleic acid circularization and amplification on a surface
US12071667B2 (en) 2020-11-04 2024-08-27 10X Genomics, Inc. Sequence analysis using meta-stable nucleic acid molecules
US12060603B2 (en) 2021-01-19 2024-08-13 10X Genomics, Inc. Methods for internally controlled in situ assays using padlock probes
US11486001B2 (en) 2021-02-08 2022-11-01 Singular Genomics Systems, Inc. Methods and compositions for sequencing complementary polynucleotides
US11884977B2 (en) 2021-03-12 2024-01-30 Singular Genomics Systems, Inc. Nanoarrays and methods of use thereof
WO2022192671A1 (en) 2021-03-12 2022-09-15 Singular Genomics Systems, Inc. Nanoarrays and methods of use thereof
US11578320B2 (en) 2021-04-27 2023-02-14 Singular Genomics Systems, Inc. High density sequencing and multiplexed priming
CN117396613A (zh) 2021-06-01 2024-01-12 10X基因组学有限公司 用于分析物检测和探针解析的方法和组合物
US20230084407A1 (en) 2021-06-02 2023-03-16 10X Genomics, Inc. Sample analysis using asymmetric circularizable probes
WO2023288225A1 (en) 2021-07-13 2023-01-19 10X Genomics, Inc. Methods for preparing polymerized matrix with controllable thickness
CA3224093A1 (en) 2021-07-30 2023-02-02 Jorge Ivan Hernandez Neuta Methods and compositions for synchronizing reactions in situ
CN118043476A (zh) 2021-08-03 2024-05-14 10X基因组学有限公司 核酸多联体及其稳定和/或压缩方法
CN117858958A (zh) 2021-08-16 2024-04-09 10X基因组学有限公司 包含分割条形码区的探针和使用方法
MX2024005030A (es) 2021-10-26 2024-05-13 Caribou Biosciences Inc Ensayo de actividad de endonucleasa en tiempo real acoplado a exonucleasa.
US20230242974A1 (en) 2021-12-27 2023-08-03 10X Genomics, Inc. Methods and compositions for rolling circle amplification
WO2023141588A1 (en) 2022-01-21 2023-07-27 10X Genomics, Inc. Multiple readout signals for analyzing a sample
WO2023164570A1 (en) 2022-02-23 2023-08-31 Insitro, Inc. Pooled optical screening and transcriptional measurements of cells comprising barcoded genetic perturbations
US11795505B2 (en) 2022-03-10 2023-10-24 Singular Genomics Systems, Inc. Nucleic acid delivery scaffolds
WO2023192616A1 (en) 2022-04-01 2023-10-05 10X Genomics, Inc. Compositions and methods for targeted masking of autofluorescence
US20240002902A1 (en) 2022-05-06 2024-01-04 10X Genomics, Inc. Analysis of antigen and antigen receptor interactions
US20240026427A1 (en) 2022-05-06 2024-01-25 10X Genomics, Inc. Methods and compositions for in situ analysis of v(d)j sequences
WO2023220300A1 (en) 2022-05-11 2023-11-16 10X Genomics, Inc. Compositions and methods for in situ sequencing
US20240035071A1 (en) 2022-06-17 2024-02-01 10X Genomics, Inc. Catalytic de-crosslinking of samples for in situ analysis
US20240101978A1 (en) 2022-08-12 2024-03-28 10X Genomics, Inc. Puma1 polymerases and uses thereof
WO2024040060A1 (en) 2022-08-16 2024-02-22 10X Genomics, Inc. Ap50 polymerases and uses thereof
WO2024081869A1 (en) 2022-10-14 2024-04-18 10X Genomics, Inc. Methods for analysis of biological samples
WO2024102736A1 (en) 2022-11-08 2024-05-16 10X Genomics, Inc. Immobilization methods and compositions for in situ detection
US20240158852A1 (en) 2022-11-16 2024-05-16 10X Genomics, Inc. Methods and compositions for assessing performance of in situ assays
US20240218437A1 (en) 2022-12-16 2024-07-04 10X Genomics, Inc. Methods and compositions for assessing performance
US20240263219A1 (en) 2023-01-06 2024-08-08 10X Genomics, Inc. Methods and compositions for in situ analysis of variant sequences
US20240263220A1 (en) 2023-02-03 2024-08-08 10X Genomics, Inc. In situ analysis of variant sequences in biological samples
WO2024196764A2 (en) 2023-03-17 2024-09-26 Nanostring Technologies, Inc. Assay for recombinase accessible chromatin and related compositions and methods

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4329461A (en) * 1980-01-11 1982-05-11 Syva Company Fluorescent thyroid hormone conjugates and their uses
DE3071373D1 (en) * 1980-10-27 1986-03-06 Syva Co Novel ether substituted fluorescein compounds as fluorescers and quenchers
GB8500360D0 (en) 1985-01-07 1985-02-13 Bristol University Of Polymers
US4855225A (en) * 1986-02-07 1989-08-08 Applied Biosystems, Inc. Method of detecting electrophoretically separated oligonucleotides
US4811218A (en) * 1986-06-02 1989-03-07 Applied Biosystems, Inc. Real time scanning electrophoresis apparatus for DNA sequencing
US4997928A (en) * 1988-09-15 1991-03-05 E. I. Du Pont De Nemours And Company Fluorescent reagents for the preparation of 5'-tagged oligonucleotides

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